Additional file 5: of Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

Figure S4. Invasion of diverse prostate recipient cells upon LO exposure (A) Invasion was performed on DU145 cells treated, as indicated in figure, with LO145 untreated or pre-exposed to blocking anti- ÎąV-integrin antibody or to CTR Ab (anti-pAKT antibody ineffective on non-permeabilized intact cells). Results, shown as % of invading cells compared to the vehicle (reported as bar at 100%), are representative of a single experiment performed in triplicates. Several experiments yielded similar results. (B) Invasion was performed at 16â h on both epithelial normal prostate cells (EPN) and PCa LNCaP cells, untreated/treated with LO from both DU145 and DU145R80 cells. Results, reported as fold change of treated cells compared to the vehicle, are representative of a single experiment performed in triplicate and several experiments yielded similar results. (PDF 42 kb)