1. Comparative Study Of Genetic Diversity, Virulence Genotype, Biofilm Formation And Antimicrobial Resistance Of Uropathogenic Escherichia coli (UPEC) Isolated From Nosocomial And Community Acquired Urinary Tract Infections
- Author
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Souza GM, Neto ERDS, da Silva AM, Iacia MVMS, Rodrigues MVP, Pereira VC, and Winkelstroter LK
- Subjects
adhesion ,control ,esbl ,uti. ,Infectious and parasitic diseases ,RC109-216 - Abstract
Gabrielle Messias De Souza, Estevan Rodrigues Dos Santos Neto, Alaor Martins da Silva, Maria Vitoria Minzoni de Souza Iacia, Marcus Vinícius Pimenta Rodrigues, Valéria Cataneli Pereira, Lizziane Kretli Winkelstroter Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, BrazilCorrespondence: Lizziane Kretli WinkelstroterHealth Sciences Faculty, University of Western Sao Paulo, 700, Jose Bongiovani St, Presidente Prudente, Sao Paulo 19050-920, BrazilTel +55 18 3229-1289Email lizzianekretli@gmail.comIntroduction: Escherichia coli is a Gram-negative opportunistic human pathogen, which has aroused considerable medical interest for being involved in cases of urinary tract infection.Aim: Characterize the E. coli isolated both in the hospital and in the community.Methodology: A total of 200 E. coli isolated in urine samples from hospital and community were evaluated in biofilm formation assay and hydrophobicity MATS method. Antimicrobial susceptibility was performed through agar-diffusion technique. Virulence and ESBL production genes were observed through the polymerase chain reaction amplification of papC, fimH, fliC, kpsMTII, blaTEM, blaCTX-M, blaSHV, and blaOXA.The phylogenetic classification was based on the pattern chuA and yjaA and the region TspE4.C2 by PCR Multiplex.Results: A higher frequency of non-adherent or poorly adherent isolates was observed in the community group. Approximately 85% of the community isolates were distributed in the highest hydrophilicity group (p0.05). About 14% of the hospital isolates were positive in the ESBL phenotypic detection test (p>0.05). Among the samples, 95% presented ESBL-encoding genes. The predominant phylogenetic group was B2 (78%). Community isolates showed a higher prevalence of virulence genes fimH, papC, and kpsMTII when compared to hospital samples.Conclusion: These data confirm the worldwide trend that isolates in the community present sometimes higher levels of virulence and antimicrobial resistance.Keywords: adhesion, control, ESBL, UTI
- Published
- 2019