29 results on '"Oades PJ"'
Search Results
2. Relation between insulin-like growth factor-I, body mass index, and clinical status in cystic fibrosis.
- Author
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Taylor AM, Bush A, Thomson A, Oades PJ, Marchant JL, Bruce-Morgan C, Holly J, Ahmed L, Dunger DB, Taylor, A M, Bush, A, Thomson, A, Oades, P J, Marchant, J L, Bruce-Morgan, C, Holly, J, Ahmed, L, and Dunger, D B
- Abstract
Objectives: Despite improved nutrition and intensive treatment, subjects with cystic fibrosis have difficulty in maintaining anabolism during intercurrent infections, which can result in reduced body mass index and impaired skeletal growth. Insulin-like growth factor-I (IGF-I) and its binding protein IGFBP3 are sensitive to changes in nutritional status. The aim of this study was to determine the relation between circulating concentrations of these peptides, body mass index, and clinical status in cystic fibrosis.Methods: Serum concentrations of IGF-I and IGFBP3 were measured in 197 subjects (108 males, 89 females; mean age 9.69 years, range 0.41-17.9 years) and these data were analysed with respect to body mass index, pubertal stage, and clinical status as assessed by Shwachman score and forced expiratory volume in one second (FEV1).Results: The mean height SD score of the children studied was -0.2 (SD 1.14) and the body mass index SD score -0.26 (1.4). The body mass index SD score declined with increasing age (r = -0.18) and paralleled changes in IGF-I concentrations, which also declined. The IGF-I SD score (calculated from control data) correlated with age (r = -0.53). The abnormalities were most obvious during late puberty, when IGF-I and IGFBP3 concentrations were significantly reduced compared with those in control subjects matched for pubertal stage. The IGF-I SD score correlated with height SD score (r = 0.14) and the decline in IGF-I concentrations with the fall in body mass index SD score (r = 0.42). IGF-I SD scores also correlated with the Shwachman score (r = 0.33) and FEV1 (r = 0.17).Conclusions: The close relation between declining IGF-I and IGFBP3 concentrations and body mass index in patients with cystic fibrosis may simply reflect poor nutritional status and insulin hyposecretion. Nevertheless, IGF-I deficiency could also contribute towards the catabolism observed in these patients, and IGF-I SD scores correlated with other measures of clinical status such as the Shwachman score and FEV1. [ABSTRACT FROM AUTHOR]- Published
- 1997
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3. Skeletal muscle contributions to reduced fitness in cystic fibrosis youth.
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Tomlinson OW, Barker AR, Fulford J, Wilson P, Shelley J, Oades PJ, and Williams CA
- Abstract
Background: Increased maximal oxygen uptake (V̇O
2max ) is beneficial in children with cystic fibrosis (CF) but remains lower compared to healthy peers. Intrinsic metabolic deficiencies within skeletal muscle (muscle "quality") and skeletal muscle size (muscle "quantity") are both proposed as potential causes for the lower V̇O2max , although exact mechanisms remain unknown. This study utilises gold-standard methodologies to control for the residual effects of muscle size from V̇O2max to address this "quality" vs. "quantity" debate., Methods: Fourteen children (7 CF vs. 7 age- and sex-matched controls) were recruited. Parameters of muscle size - muscle cross-sectional area (mCSA) and thigh muscle volume (TMV) were derived from magnetic resonance imaging, and V̇O2max obtained via cardiopulmonary exercise testing. Allometric scaling removed residual effects of muscle size, and independent samples t -tests and effect sizes (ES) identified differences between groups in V̇O2max , once mCSA and TMV were controlled for., Results: V̇O2max was shown to be lower in the CF group, relative to controls, with large ES being identified when allometrically scaled to mCSA (ES = 1.76) and TMV (ES = 0.92). Reduced peak work rate was also identified in the CF group when allometrically controlled for mCSA (ES = 1.18) and TMV (ES = 0.45)., Conclusions: A lower V̇O2max was still observed in children with CF after allometrically scaling for muscle size, suggesting reduced muscle "quality" in CF (as muscle "quantity" is fully controlled for). This observation likely reflects intrinsic metabolic defects within CF skeletal muscle., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Tomlinson, Barker, Fulford, Wilson, Shelley, Oades and Williams.)- Published
- 2023
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4. Quantification of thigh muscle volume in children and adolescents using magnetic resonance imaging.
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Tomlinson OW, Barker AR, Fulford J, Wilson P, Oades PJ, and Williams CA
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- Adolescent, Bias, Child, Cystic Fibrosis genetics, Cystic Fibrosis physiopathology, Female, Humans, Male, Muscle, Skeletal anatomy & histology, Organ Size, Thigh diagnostic imaging, Magnetic Resonance Imaging methods, Muscle, Skeletal diagnostic imaging
- Abstract
Abstract Estimating muscle volume (MV) using variable numbers of cross-sectional area (CSA) slices obtained from magnetic resonance imaging (MRI) introduces an error that is known in adults, but not in children and adolescents, whereby body sizes differ due to growth and maturation. Therefore, 15 children and adolescents (11 males, 14.8 ± 2.1 years) underwent MRI scans of the right thigh using a 1.5 T scanner to establish this error. A criterion MV was determined by tracing around and summing all CSAs, with MV subsequently estimated using every second, third, fourth and fifth CSA slice. Bland-Altman plots identified mean bias and limits of agreement (LoA) between methods. Error rates between 1.0 and 10.4% were seen between criterion and estimated MV. Additional analyses identified an impact of formulae selection, with a cylindrical formula preferred to a truncated cone. To counter high error between criterion and estimated MV due to the discrepancies in the number of CSA slices analysed, length-matched criterion volumes were established, with reduced error rates (0.5-2.0%) being produced as a result. CSA at 50% thigh-length also predicted MV, producing a high error (13.8-39.6%). Pearson's correlation coefficients determined relationships between error and measures of body size/composition, with all body size/composition measures being correlated ( r = -0.78-0.86, p < 0.05) with the error between criterion and estimated MV. To conclude, MV can be accurately estimated using fewer CSA slices. However, the associated error must be considered when calculating MV in children and adolescents, as body size biases estimates.
- Published
- 2020
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5. Analysis of oxygen uptake efficiency parameters in young people with cystic fibrosis.
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Tomlinson OW, Barker AR, Chubbock LV, Stevens D, Saynor ZL, Oades PJ, and Williams CA
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- Adolescent, Exercise physiology, Exercise Test methods, Exercise Tolerance physiology, Humans, Lung metabolism, Lung physiopathology, Male, Respiratory Function Tests methods, Cystic Fibrosis metabolism, Cystic Fibrosis physiopathology, Oxygen metabolism, Oxygen Consumption physiology
- Abstract
Purpose: This study characterised oxygen uptake efficiency (OUE) in children with mild-to-moderate cystic fibrosis (CF). Specifically, it investigated (1) the utility of OUE parameters as potential submaximal surrogates of peak oxygen uptake ([Formula: see text]), and (2) the relationship between OUE and disease severity., Methods: Cardiopulmonary exercise test (CPET) data were collated from 72 children [36 CF, 36 age- and sex-matched controls (CON)], with OUE assessed as its highest 90-s average (plateau; OUEP), the gas exchange threshold (OUE
GET ) and respiratory compensation point (OUERCP ). Pearson's correlation coefficients, independent t tests and factorial ANOVAs assessed differences between groups and the use of OUE measures as surrogates for [Formula: see text]., Results: A significant (p < 0.05) reduction in allometrically scaled [Formula: see text] and all OUE parameters was found in CF. Significant (p < 0.05) correlations between measurements of OUE and allometrically scaled [Formula: see text], were observed in CF (r = 0.49-0.52) and CON (r = 0.46-0.52). Furthermore, measures of OUE were significantly (p < 0.05) correlated with pulmonary function (FEV1%predicted ) in CF (r = 0.38-0.46), but not CON (r = -0.20-0.14). OUEP was able to differentiate between different aerobic fitness tertiles in CON but not CF., Conclusions: OUE parameters were reduced in CF, but were not a suitable surrogate for [Formula: see text]. Clinical teams should, where possible, continue to utilise maximal CPET parameters to measure aerobic fitness in children and adolescents with CF. Future research should assess the prognostic utility of OUEP as it does appear sensitive to disease status and severity.- Published
- 2018
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6. The oxygen uptake efficiency slope is not a valid surrogate of aerobic fitness in cystic fibrosis.
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Williams CA, Tomlinson OW, Chubbock LV, Stevens D, Saynor ZL, Oades PJ, and Barker AR
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- Adolescent, Child, Exercise physiology, Female, Humans, Male, Oxygen metabolism, Cystic Fibrosis metabolism, Exercise Test, Oxygen Consumption
- Abstract
Background: Maximal cardiopulmonary exercise testing is recommended on an annual basis for children with cystic fibrosis (CF), due to clinically useful prognostic information provided by maximal oxygen uptake (V̇O
2max ). However, not all patients are able, or willing, to reach V̇O2max , and therefore submaximal alternatives are required. This study explored the validity of the oxygen uptake efficiency slope (OUES) as a submaximal measure of V̇O2max in children and adolescents with CF., Methods: Data were collated from 72 cardiopulmonary exercise tests (36 CF, 36 controls), with OUES determined relative to maximal and submaximal parameters of exercise intensity, time, and individual metabolic thresholds. Pearson's correlation coefficients, independent t-tests, and factorial ANOVAs were used to determine validity., Results: Significant (P < 0.05) correlations with V̇O2max were observed for most expressions of OUES, but were consistently weaker in CF (r = 0.30-0.47) when compared to CON (r = 0.58-0.89). Mean differences for all OUES parameters between groups were not significant (P > 0.05). When split by V̇O2max tertiles, minimal significant differences were found between, and within, groups for OUES, indicating poor discrimination of V̇O2max ., Conclusions: The OUES is not a valid (sub) maximal measure of V̇O2max in children and adolescents with mild-to-moderate CF. Clinicians should continue to use maximal markers (ie, V̇O2max ) of exercise capacity., (© 2017 Wiley Periodicals, Inc.)- Published
- 2018
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7. Measurement of V̇o 2max in clinical groups is feasible and necessary.
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Williams CA, Saynor ZL, Barker AR, Oades PJ, and Tomlinson OW
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- Oxygen, Exercise Test, Oxygen Consumption
- Published
- 2017
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8. Scaling the Oxygen Uptake Efficiency Slope for Body Size in Cystic Fibrosis.
- Author
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Tomlinson OW, Barker AR, Oades PJ, and Williams CA
- Subjects
- Body Height, Body Mass Index, Body Surface Area, Exercise Test, Humans, Oxygen Consumption physiology, Body Size physiology, Cystic Fibrosis physiopathology, Oxygen physiology, Respiration
- Abstract
Purpose: The aim of this study was to describe the relationship between body size and oxygen uptake efficiency slope (OUES) in pediatric patients with cystic fibrosis (CF) and healthy controls (CON), to identify appropriate scaling procedures to adjust the influence of body size upon OUES., Methods: The OUES was derived using maximal and submaximal points from cardiopulmonary exercise testing in 72 children (36 CF and 36 CON). OUES was subsequently scaled for stature, body mass (BM), and body surface area (BSA) using ratio-standard (Y/X) and allometric (Y/X) methods. Pearson's correlation coefficients were used to determine the relationship between body size and OUES., Results: When scaled using the ratio-standard method, OUES had a significant positive relationship with stature (r = 0.54, P < 0.001) and BSA (r = 0.25, P = 0.031) and significant negative relationship with BM (r = -0.38, P = 0.016) in the CF group. Combined allometric exponents (b) for CF and CON were stature 3.00, BM 0.86, and BSA 1.40. A significant negative correlation was found between OUES and stature in the CF group when scaled allometrically (r = -0.37, P = 0.027). Nonsignificant (P > 0.05) correlations for the whole group were found between OUES and allometrically scaled BM (CF r = -0.25, CON, r = 0.15) and BSA (CF r = -0.27, CON r = 0.13)., Conclusions: Only allometric scaling of either BM or BSA, and not ratio-standard scaling, successfully eliminates the influence of body size upon OUES. Therefore, this enables a more direct comparison of the OUES between patients with CF and healthy controls.
- Published
- 2017
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9. Impaired Pulmonary V˙O2 Kinetics in Cystic Fibrosis Depend on Exercise Intensity.
- Author
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Saynor ZL, Barker AR, Oades PJ, and Williams CA
- Subjects
- Adolescent, Cardiac Output physiology, Case-Control Studies, Child, Exercise Test, Female, Heart Rate physiology, Humans, Male, Muscle, Skeletal metabolism, Oxygen metabolism, Oxygen Consumption physiology, Pulmonary Gas Exchange physiology, Stroke Volume physiology, Cystic Fibrosis physiopathology, Exercise physiology, Lung physiology, Oxygen physiology
- Abstract
Purpose: This study aimed to investigate the effects of mild-to-moderate cystic fibrosis (CF) on the pulmonary oxygen uptake (V˙O2) kinetics of seven pediatric patients (13.5 ± 2.8 yr) versus seven healthy matched controls (CON; 13.6 ± 2.4 yr). We hypothesized that CF would slow the V˙O2 kinetic response at the onset of moderate (MOD) and very heavy (VH) intensity cycling., Methods: Changes in breath-by-breath V˙O2, near-infrared spectroscopy-derived muscle deoxygenation ([HHb]) at the vastus lateralis muscle and thoracic bioelectrical impedance-derived heart rate (HR), stroke volume index, and cardiac index were measured during repeat transitions to MOD (90% of the gas exchange threshold) and VH (Δ60%) intensity cycling exercise., Results: During MOD, the phase II V˙O2 τ (P = 0.84, effect size [ES] = 0.11) and the overall mean response time (MRT) (P = 0.52, ES = 0.11) were not significantly slower in CF versus CON. However, during VH exercise, the phase II V˙O2 τ (P = 0.02, ES = 1.28) and MRT (P = 0.01, ES = 1.40) were significantly slower in CF. Cardiac function, central O2 delivery (stroke volume index and cardiac index), and muscle [HHb] kinetics were unaltered in CF. However, the arteriovenous O2 content difference ((Equation is included in full-text article.)) was reduced during VH at 30 s (P = 0.03, ES = 0.37), with a trend for reduced levels at 0 s (P = 0.07, ES = 0.25), 60 s (P = 0.05, ES = 0.28), and 120 s (P = 0.07, ES = 0.25) in CF. Furthermore, (Equation is included in full-text article.)significantly correlated with the VH phase II V˙O2 τ (r = -0.85, P = 0.02) and MRT (r = -0.79, P = 0.03) in CF only., Conclusion: Impairments in muscle oxidative metabolism during constant work rate exercise are intensity dependent in young people with mild-to-moderate CF. Specifically, V˙O2 kinetics are slowed during VH but not MOD cycling and appear to be mechanistically linked to impaired muscle O2 extraction and utilization.
- Published
- 2016
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10. Exercise capacity following a percutaneous endoscopic gastrostomy in a young female with cystic fibrosis: a case report.
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Tomlinson OW, Barker AR, Oades PJ, and Williams CA
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- Body Mass Index, Child, Cystic Fibrosis diet therapy, Cystic Fibrosis surgery, Endoscopy methods, Enteral Nutrition methods, Exercise Test methods, Female, Follow-Up Studies, Gastrostomy methods, Humans, Nutrition Disorders etiology, Outcome Assessment, Health Care, Respiratory Function Tests methods, Body Weight physiology, Cystic Fibrosis complications, Endoscopy adverse effects, Enteral Nutrition adverse effects, Exercise physiology, Gastrostomy adverse effects, Nutrition Disorders surgery
- Abstract
Cystic fibrosis (CF) is a genetic condition affecting the respiratory and gastrointestinal systems, with patients experiencing problems maintaining weight, especially during rapid growth periods such as puberty. The aim of this case report was to monitor the effect of gastrostomy insertion and implementation of overnight supplemental feeding upon clinical outcomes, including body mass index (BMI), lung function (FEV
1 ), and exercise-related variables (maximal oxygen uptake [VO2max ] and ventilatory efficiency [VE /VO2 ]) in an 11-year-old female with CF Combined incremental and supramaximal exercise testing to exhaustion was performed at four time points: 3 months prior to the procedure (T1), 2 days prior to (T2), 4 months (T3), and 1 year following the procedure (T4). Improvements following gastrostomy insertion were observed at the 1 year follow-up with regards to BMI (+20%); whereas absolute VO2max remained stable and lung function fluctuated throughout the period of observation. Declines in function with regards to body weight relative VO2max (-16.3%) and oxygen uptake efficiency (+7.5%) were observed during this period. This case report is the first to consider exercise-related clinical outcomes in assessing the effect of implementing gastrostomy feeding in CF The varied direction and magnitude of the associations between variables shows that further investigations are required., (© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.)- Published
- 2016
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11. Validity and Reliability Concerns Associated with Cardiopulmonary Exercise Testing Young People with Cystic Fibrosis.
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Saynor ZL, Barker AR, Oades PJ, Tomlinson OW, and Williams CA
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- Humans, Oxygen Consumption, Reproducibility of Results, Cystic Fibrosis, Exercise Test
- Published
- 2016
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12. Characterization of human disease phenotypes associated with mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, ADAR, and IFIH1.
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Crow YJ, Chase DS, Lowenstein Schmidt J, Szynkiewicz M, Forte GM, Gornall HL, Oojageer A, Anderson B, Pizzino A, Helman G, Abdel-Hamid MS, Abdel-Salam GM, Ackroyd S, Aeby A, Agosta G, Albin C, Allon-Shalev S, Arellano M, Ariaudo G, Aswani V, Babul-Hirji R, Baildam EM, Bahi-Buisson N, Bailey KM, Barnerias C, Barth M, Battini R, Beresford MW, Bernard G, Bianchi M, Billette de Villemeur T, Blair EM, Bloom M, Burlina AB, Carpanelli ML, Carvalho DR, Castro-Gago M, Cavallini A, Cereda C, Chandler KE, Chitayat DA, Collins AE, Sierra Corcoles C, Cordeiro NJ, Crichiutti G, Dabydeen L, Dale RC, D'Arrigo S, De Goede CG, De Laet C, De Waele LM, Denzler I, Desguerre I, Devriendt K, Di Rocco M, Fahey MC, Fazzi E, Ferrie CD, Figueiredo A, Gener B, Goizet C, Gowrinathan NR, Gowrishankar K, Hanrahan D, Isidor B, Kara B, Khan N, King MD, Kirk EP, Kumar R, Lagae L, Landrieu P, Lauffer H, Laugel V, La Piana R, Lim MJ, Lin JP, Linnankivi T, Mackay MT, Marom DR, Marques Lourenço C, McKee SA, Moroni I, Morton JE, Moutard ML, Murray K, Nabbout R, Nampoothiri S, Nunez-Enamorado N, Oades PJ, Olivieri I, Ostergaard JR, Pérez-Dueñas B, Prendiville JS, Ramesh V, Rasmussen M, Régal L, Ricci F, Rio M, Rodriguez D, Roubertie A, Salvatici E, Segers KA, Sinha GP, Soler D, Spiegel R, Stödberg TI, Straussberg R, Swoboda KJ, Suri M, Tacke U, Tan TY, te Water Naude J, Wee Teik K, Thomas MM, Till M, Tonduti D, Valente EM, Van Coster RN, van der Knaap MS, Vassallo G, Vijzelaar R, Vogt J, Wallace GB, Wassmer E, Webb HJ, Whitehouse WP, Whitney RN, Zaki MS, Zuberi SM, Livingston JH, Rozenberg F, Lebon P, Vanderver A, Orcesi S, and Rice GI
- Subjects
- Genetic Association Studies, Genotype, Humans, Interferon-Induced Helicase, IFIH1, Interferons blood, Interferons cerebrospinal fluid, Pterins cerebrospinal fluid, SAM Domain and HD Domain-Containing Protein 1, Adenosine Deaminase genetics, Autoimmune Diseases of the Nervous System diagnosis, Autoimmune Diseases of the Nervous System genetics, DEAD-box RNA Helicases genetics, Exodeoxyribonucleases genetics, Monomeric GTP-Binding Proteins genetics, Mutation, Nervous System Malformations diagnosis, Nervous System Malformations genetics, Phenotype, Phosphoproteins genetics, Ribonuclease H genetics
- Abstract
Aicardi-Goutières syndrome is an inflammatory disease occurring due to mutations in any of TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, ADAR or IFIH1. We report on 374 patients from 299 families with mutations in these seven genes. Most patients conformed to one of two fairly stereotyped clinical profiles; either exhibiting an in utero disease-onset (74 patients; 22.8% of all patients where data were available), or a post-natal presentation, usually within the first year of life (223 patients; 68.6%), characterized by a sub-acute encephalopathy and a loss of previously acquired skills. Other clinically distinct phenotypes were also observed; particularly, bilateral striatal necrosis (13 patients; 3.6%) and non-syndromic spastic paraparesis (12 patients; 3.4%). We recorded 69 deaths (19.3% of patients with follow-up data). Of 285 patients for whom data were available, 210 (73.7%) were profoundly disabled, with no useful motor, speech and intellectual function. Chilblains, glaucoma, hypothyroidism, cardiomyopathy, intracerebral vasculitis, peripheral neuropathy, bowel inflammation and systemic lupus erythematosus were seen frequently enough to be confirmed as real associations with the Aicardi-Goutieres syndrome phenotype. We observed a robust relationship between mutations in all seven genes with increased type I interferon activity in cerebrospinal fluid and serum, and the increased expression of interferon-stimulated gene transcripts in peripheral blood. We recorded a positive correlation between the level of cerebrospinal fluid interferon activity assayed within one year of disease presentation and the degree of subsequent disability. Interferon-stimulated gene transcripts remained high in most patients, indicating an ongoing disease process. On the basis of substantial morbidity and mortality, our data highlight the urgent need to define coherent treatment strategies for the phenotypes associated with mutations in the Aicardi-Goutières syndrome-related genes. Our findings also make it clear that a window of therapeutic opportunity exists relevant to the majority of affected patients and indicate that the assessment of type I interferon activity might serve as a useful biomarker in future clinical trials., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2015
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13. Airflow limitation following cardiopulmonary exercise testing and heavy-intensity intermittent exercise in children with cystic fibrosis.
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Stevens D, Oades PJ, and Williams CA
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- Adolescent, Child, Female, Forced Expiratory Volume physiology, Humans, Male, Cystic Fibrosis physiopathology, Exercise physiology, Exercise Test, Respiration Disorders physiopathology
- Abstract
Unlabelled: The clinical importance of exercise testing and training in the healthcare management of young patients with cystic fibrosis (CF) is growing. The aim of the present study was to determine the incidence of airflow limitation following cardiopulmonary exercise testing (CPET) and heavy-intensity intermittent exercise (HIIE) in young patients with CF. Nineteen young patients with CF and respective paired-matched controls performed CPET and HIIE on separate days. Forced expiratory volume in one second (FEV1) was measured pre- and post each exercise modality. A fall in FEV1 of 10 % or greater was used to define airflow limitation. The incidence of airflow limitation was significantly greater in the CF group than in the controls following CPET (32 vs. 5 %; p = 0.03); however, no significant difference in the incidence of airflow limitation was shown following HIIE between the CF group and controls (11 vs. 16 %; p = 0.64)., Conclusion: Our data show that the incidence of airflow limitation following CPET in young patients with CF is high. Therefore, clinicians may wish to identify whether young CF patients experience airflow limitation following strenuous exercise, such as CPET, before it is performed. However, HIIE carries a low risk for airflow limitation and may be prescribed as a safe, yet effective exercise modality for young patients with CF.
- Published
- 2015
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14. Enteroviral meningoencephalitis in an infant: an increasingly recognised infection.
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Oades PJ and Ladhani S
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- Cerebrospinal Fluid virology, Diagnosis, Differential, Enterovirus B, Human genetics, Enterovirus Infections virology, Fatal Outcome, Gestational Age, Humans, Infant, Newborn, Male, Meningoencephalitis virology, Polymerase Chain Reaction, RNA, Viral isolation & purification, Enterovirus B, Human isolation & purification, Enterovirus Infections diagnosis, Meningoencephalitis diagnosis
- Published
- 2015
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15. Impaired aerobic function in patients with cystic fibrosis during ramp exercise.
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Saynor ZL, Barker AR, Oades PJ, and Williams CA
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- Adolescent, Anthropometry, Blood Pressure, Child, Cystic Fibrosis physiopathology, Exercise Test, Female, Hemoglobins metabolism, Humans, Male, Oxygen blood, Partial Pressure, Pulmonary Gas Exchange, Quadriceps Muscle blood supply, Cystic Fibrosis metabolism, Exercise physiology, Oxygen Consumption, Quadriceps Muscle metabolism
- Abstract
Purpose: This study aimed to document the matching of muscle O2 delivery to O2 use in young patients with cystic fibrosis (CF) from muscle deoxygenation (HHb) dynamics during ramp exercise., Methods: Ten patients with stable, mild-to-moderate CF (12.7 ± 2.8 yr) and 10 healthy controls (CON, 12.8 ± 2.8 yr) completed a combined ramp and supramaximal cycling test to determine maximal O2 uptake (V˙O2max). Changes in gas exchange and ventilation, HR, and m. vastus lateralis HHb (near-infrared spectroscopy) were assessed. Δ[HHb]-work rate and Δ[HHb]-V˙O2 profiles were normalized and fit using a sigmoid function., Results: Aerobic function was impaired in CF, indicated by very likely reduced fat-free mass-normalized V˙O2max (mean difference, ±90% confidence interval: -7.9 mL·kg·min, ±6.1), very likely lower V˙O2 gain (-1.44 mL·min·W, ±1.12), and a likely slower V˙O2 mean response time (11 s, ±13). An unclear effect was found upon the absolute and relative work rate (-14 W, ±44, and -0.7% peak power output, ±12.0, respectively) and the absolute and percentage (-0.10 L·min, ±0.43, and 3.3% V˙O2max, ±6.0) V˙O2 corresponding to 50% Δ[HHb] amplitude, respectively, between groups. However, arterial oxygen saturation (SpO2) was very likely lower in CF (-1%, ±1) and demonstrated moderate-to-very large relations with parameters of aerobic function., Conclusions: Young patients with mild-to-moderate CF present with impaired aerobic function during ramp incremental cycling exercise. Because the rate of fractional O2 extraction during ramp cycling exercise was not altered by CF, yet SpO2 was lower, the present findings support the notion of centrally mediated oxygen delivery to principally limit the aerobic function of pediatric patients with CF during ramp incremental cycling exercise.
- Published
- 2014
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16. The effect of ivacaftor in adolescents with cystic fibrosis (G551D mutation): an exercise physiology perspective.
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Saynor ZL, Barker AR, Oades PJ, and Williams CA
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- Adolescent, Aminophenols administration & dosage, Aminophenols adverse effects, Body Weights and Measures, Female, Humans, Male, Quinolones administration & dosage, Quinolones adverse effects, Respiratory Function Tests, Aminophenols therapeutic use, Cystic Fibrosis drug therapy, Cystic Fibrosis rehabilitation, Cystic Fibrosis Transmembrane Conductance Regulator agonists, Exercise Test, Quinolones therapeutic use
- Abstract
Purpose: The purpose of this report was to evaluate the influence of 12 weeks of ivacaftor treatment on the aerobic function of 2 teenage patients with cystic fibrosis (CF; ΔF508/G551D) using a maximal cardiopulmonary exercise test., Summary of Key Points: One patient, with relatively mild disease, demonstrated no clinically meaningful changes in maximal oxygen uptake ((Equation is included in full-text article.)O2max). However, in the second case, with more established lung disease on imaging, (Equation is included in full-text article.)O2max improved by approximately 30%, an improvement out of proportion with early lung function changes. This improvement resulted from increased muscle oxygen delivery and extraction., Statement of Conclusions: Cardiopulmonary exercise testing can monitor the extent and cause(s) of change following interventions such as ivacaftor, with the potential to identify functional changes independent from spirometry indices., Recommendations for Clinical Practice: Cardiopulmonary exercise testing represents an important and comprehensive clinical assessment tool, and its use as an outcome measure in the functional assessment of patients with CF is encouraged.
- Published
- 2014
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17. Reproducibility of maximal cardiopulmonary exercise testing for young cystic fibrosis patients.
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Saynor ZL, Barker AR, Oades PJ, and Williams CA
- Subjects
- Adolescent, Child, Female, Humans, Male, Oxygen Consumption, Pulmonary Gas Exchange, Reproducibility of Results, Cystic Fibrosis physiopathology, Exercise Test
- Abstract
Background: The reproducibility of cardiopulmonary exercise testing (CPET) has not been established in young cystic fibrosis (CF) patients using a valid protocol., Methods: Thirteen 7-18 year olds completed three CPETs, separated by 48 h and 4-6 weeks. CPET involved a ramp-incremental cycling test with supramaximal verification., Results: Maximal oxygen uptake was repeatedly determined with no learning effect and typical errors expressed as a coefficient of variation (TE(CV%)) of 9.3% (48 h) and 13.3% (4-6 weeks). The reproducibility of additional parameters of aerobic function [gas exchange threshold (TE(CV%): 11.2%, 16.8%); VO2 mean response time (TE(CV%): 37.8%, 89.4%); VO2 gain (TE(CV%): 17.4%, 24.5%)] and clinical utility [e.g. SaO2% (TE(CV%): 2.2%, 3.1%); ventilatory drive (V(E)/VCO2-slope) (TE(CV%): 7.8%, 17.7%)] was also established over the short- and the medium-term, respectively., Conclusion: These results establish limits of variability to determine meaningful changes over the short- and the medium-term for CPET outcomes in young CF patients., (Copyright © 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.)
- Published
- 2013
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18. A protocol to determine valid V˙O2max in young cystic fibrosis patients.
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Saynor ZL, Barker AR, Oades PJ, and Williams CA
- Subjects
- Adolescent, Child, Clinical Protocols, Cross-Sectional Studies, Female, Humans, Male, Cystic Fibrosis metabolism, Exercise Test methods, Oxygen Consumption
- Abstract
Objectives: Measuring aerobic fitness (V˙O2max) via a maximal cardiopulmonary exercise test is an important clinical tool in cystic fibrosis. This study sought to establish: (1) the validity of traditional criteria to verify maximal efforts during a ramp cardiopulmonary exercise test; and (2) whether V˙O2 measured during an exhaustive cardiopulmonary exercise test represents a valid V˙O2max in paediatric patients, using a subsequent exhaustive supramaximal (Smax) exercise test., Design: Cross-sectional., Methods: Fourteen patients (7-18 years; 10 males) completed an exhaustive ramp test to determine V˙O2max. Following 15-min recovery, Smax (110% ramp peak power output) was performed., Results: Ramp test V˙O2peak was significantly higher than V˙O2 documented at traditional endpoint criteria, including a RER of 1.00 (0.99±0.47 L min(-1) vs. 1.83±0.78 L min(-1), p<0.001) and 1.10 (1.36±0.59 L min(-1) vs. 1.83±0.78 L min(-1), p<0.001), despite 100% of patients satisfying these two criteria. Only 23% and 75% of patients satisfied the 95% age-predicted heart rate (HR) maximum and 180 b min(-1) criteria. Whilst mean ramp and Smax V˙O2peak were not significantly different (1.83±0.78 L min(-1) vs. 1.82±0.67 L min(-1); p=0.88), at the individual level Smax elicited a 'meaningful' (>9%) increase in V˙O2peak (range 9.9-38.3%) compared with V˙O2peak from the ramp test in 3 of 14 cases (21.4%)., Conclusions: Traditional criteria significantly underestimate V˙O2max in young cystic fibrosis patients. Conversely, Smax can confirm when 'true' V˙O2max is achieved. The use of Smax following cardiopulmonary exercise test represents an appropriate method to measure V˙O2max in young cystic fibrosis patients., (Copyright © 2013 Sports Medicine Australia. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
19. Exercise metabolism during moderate-intensity exercise in children with cystic fibrosis following heavy-intensity exercise.
- Author
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Stevens D, Oades PJ, Armstrong N, and Williams CA
- Subjects
- Adolescent, Cardiovascular System physiopathology, Child, Cystic Fibrosis blood, Cystic Fibrosis therapy, Exercise Test, Exercise Tolerance, Female, Heart Rate, Humans, Lactic Acid blood, Male, Oxygen Consumption, Respiratory Rate, Respiratory System physiopathology, Severity of Illness Index, Cystic Fibrosis metabolism, Cystic Fibrosis physiopathology, Exercise, Muscle, Skeletal metabolism, Muscle, Skeletal physiopathology, Physical Exertion
- Abstract
Muscle metabolism is increased following exercise in healthy individuals, affecting exercise metabolism during subsequent physical work. We hypothesized that following heavy-intensity exercise (HIE), disease factors in children with cystic fibrosis (CF) would further exacerbate exercise metabolism and perceived exertion during subsequent exercise. Nineteen children with CF (age, 13.4 ± 3.1 years; 10 female) and 19 healthy controls (age, 13.8 ± 3.5 years; 10 female) performed 10 bouts of HIE interspersed with 1 min of recovery between each bout. Three minutes later participants completed a 10-min moderate-intensity exercise (MIE) test (test 1). The MIE test was subsequently repeated 1 h (test 2) and 24 h (test 3) later. Each MIE test was identical and participants exercised at individualized work rates, calibrated by an initial graded maximal cardiopulmonary exercise test, while metabolic and perceived exertion measurements were taken. Following HIE, mixed-model ANOVAs showed a significant difference in oxygen uptake (VO₂) and rating of perceived exertion (RPE) between the 2 groups across the MIE tests (p < 0.01). In controls, VO₂ (L·min⁻¹) and RPE decreased significantly from test 1 to test 2 (p < 0.01) and test 2 to test 3 (p < 0.05). However, in children with CF, VO₂ (L·min⁻¹) increased significantly from test 1 to test 2 (p < 0.01), while RPE did not differ, both VO₂ and RPE decreased significantly from test 2 to test 3 (p < 0.01). In conclusion, following HIE the metabolic and perceptual responses to MIE in both groups decreased 24 h later during test 3. These data show that children with mild-to-moderate CF have the capability to perform HIE and 24 h allows sufficient time for recovery.
- Published
- 2011
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20. A survey of exercise testing and training in UK cystic fibrosis clinics.
- Author
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Stevens D, Oades PJ, Armstrong N, and Williams CA
- Subjects
- Adult, Child, Humans, Surveys and Questionnaires, United Kingdom, Cystic Fibrosis diagnosis, Cystic Fibrosis rehabilitation, Exercise Test statistics & numerical data, Outpatient Clinics, Hospital, Physical Education and Training
- Abstract
Background: Exercise testing is a valuable prognostic tool and exercise training has many health benefits in cystic fibrosis (CF). The objective of this study was to survey the provision of exercise testing and training in UK CF clinics., Methods: A three-page questionnaire was used to determine the extent of, scope and importance assigned to exercise testing and training., Results: Data from returned questionnaires showed that 38.9% of paediatric and 27.8% of adult patients had performed an exercise test in the preceding 12 months, most as part of an annual review process. Pulmonary rehabilitation programmes were accessible in only 31.3% of clinics, and only 26.0% provide exercise training programmes. When assigning importance for exercise testing on a scale from 1 'not important' to 5 'very important', the mean and median respondent scores were 3.5 and 4.0, respectively, and for the importance of training were 4.0 and 4.0, respectively., Conclusions: Despite the level of importance given to exercise testing and training by healthcare providers, exercise is underused as either an assessment tool or therapeutic intervention in the healthcare of patients with CF in the UK., (Copyright © 2010 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
21. Early oxygen uptake recovery following exercise testing in children with chronic chest diseases.
- Author
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Stevens D, Oades PJ, Armstrong N, and Williams CA
- Subjects
- Adolescent, Case-Control Studies, Child, Exercise Test, Female, Humans, Male, Pulmonary Ventilation physiology, Bronchiectasis physiopathology, Cystic Fibrosis physiopathology, Exercise Tolerance physiology, Oxygen Consumption physiology
- Abstract
The value of exercise testing as an objective measure of disease severity in patients with chronic chest diseases (CCD) is becoming increasingly recognized. The aim of this study was to investigate changes in oxygen uptake (VO2) during early recovery following maximal cardiopulmonary exercise testing (CPXT) in relation to functional capacity and markers of disease severity. Twenty-seven children with CCD (age 12.7 +/- 3.1 years; 17 female) [19 children with Cystic fibrosis (CF) (age 13.4 +/- 3.1 years; 10 female) and 8 with other stable non-CF chest diseases (NON-CF) (age 11.1 +/- 2.2 years; 7 female)] and 27 healthy controls (age 13.2 +/- 3.3 years; 17 female) underwent CPXT on a cycle ergometer. On-line respiratory gas analysis measured VO2 before and during CPXT to peak VO2) (VO2(peak)), and during the first 10 min of recovery. Early VO2 recovery was quantified by the time (sec) to reach 50% of the VO2 (peak) value. Early VO2 recovery was correlated against spirometry [forced expiratory volume in 1 sec (FEV(1)) and forced expiratory flow between 25% and 75% of the forced vital capacity (FEF(25-75))] and aerobic fitness (VO2)(peak)) as a measure of functional capacity. Disease severity was graded in the CF patients by the Shwachman score (SS). Compared to controls, children with CCD demonstrated a significantly reduced VO2(peak) (P = 0.011), FEV(1) (P < 0.001), FEF(25-75) (P < 0.001), and a significantly prolonged early (VO2) recovery (P = 0.024). In the CF patients the SS was significantly correlated with early VO2 recovery (r = -0.63, P = 0.004), FEV(1) (r = 0.72, P = 0.001), and FEF(25-75) (r = 0.57, P = 0.011). In the children with CCD, FEV(1), FEF(25-75), and BMI were not significantly correlated with VO2(peak) or early VO2 recovery. Lung function does not necessarily reflect aerobic fitness and the ability to recover from exercise in these patients. A significant relationship was found between VO2(peak) and early VO2 recovery (r = -0.39, P = 0.044) in the children with CCD, showing that a greater aerobic fitness corresponded with a faster recovery., ((c) 2009 Wiley-Liss, Inc.)
- Published
- 2009
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22. Coma due to cannabis toxicity in an infant.
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Appelboam A and Oades PJ
- Subjects
- Female, Humans, Infant, Marijuana Smoking, Parents education, Parents psychology, Police, Risk Assessment, Social Work, Cannabis toxicity, Coma chemically induced, Infant Welfare
- Abstract
In young children, cannabis ingestion resulting in coma is very rare. Only nine cases have been reported in the literature and most have occurred in inquisitive toddlers. We review the cases to date and report the youngest recorded case of coma due to cannabis ingestion in an 11-month-old girl. This case highlights the importance of considering toxicological causes and the parental recreational drug history in young children who present with an altered level of consciousness. Child protection considerations are also discussed.
- Published
- 2006
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23. Clinical improvement in cystic fibrosis with early insulin treatment.
- Author
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Dobson L, Hattersley AT, Tiley S, Elworthy S, Oades PJ, and Sheldon CD
- Subjects
- Adult, Cystic Fibrosis complications, Cystic Fibrosis physiopathology, Diabetes Complications, Diabetes Mellitus physiopathology, Female, Forced Expiratory Volume drug effects, Humans, Male, Treatment Outcome, Vital Capacity drug effects, Cystic Fibrosis drug therapy, Diabetes Mellitus drug therapy, Hypoglycemic Agents therapeutic use, Insulin therapeutic use
- Published
- 2002
- Full Text
- View/download PDF
24. Subclinical colonic thickening.
- Author
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Oades PJ, Rosenthal M, Mac Sweeney EJ, and Bush A
- Subjects
- Adolescent, Child, Child, Preschool, Colon pathology, Colonic Diseases pathology, Cystic Fibrosis diet therapy, Cystic Fibrosis pathology, Enzymes adverse effects, Fibrosis, Humans, Infant, Colonic Diseases etiology, Cystic Fibrosis complications
- Published
- 1996
25. Colonic stricture in a boy with cystic fibrosis.
- Author
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Ong PS, Oades PJ, Bush A, and Brereton RJ
- Subjects
- Child, Preschool, Colonic Diseases pathology, Cystic Fibrosis pathology, Humans, Intestinal Obstruction pathology, Intestines pathology, Male, Microspheres, Colonic Diseases etiology, Cystic Fibrosis complications, Intestinal Obstruction etiology, Pancreatic Hormones administration & dosage, Tablets, Enteric-Coated adverse effects
- Abstract
Many problems may arise within the gastrointestinal tract of patients with cystic fibrosis. We report a new cause of subacute intestinal obstruction due to a fibrotic stricture of the ascending colon in a child with cystic fibrosis. Treatment was with a right hemicolectomy. There has been no recurrence after nine months follow-up. Recent similar cases suggest that this new pathology is linked to the use of enteric-coated high-strength pancreatin microspheres.
- Published
- 1995
- Full Text
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26. Relation of thickening of colon wall to pancreatic-enzyme treatment in cystic fibrosis.
- Author
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Mac Sweeney EJ, Oades PJ, Buchdahl R, Rosenthal M, and Bush A
- Subjects
- Adolescent, Adult, Amylases administration & dosage, Child, Child, Preschool, Colon diagnostic imaging, Colon drug effects, Cystic Fibrosis diagnostic imaging, Cystic Fibrosis pathology, Endopeptidases administration & dosage, Fibrosis, Humans, Infant, Infant, Newborn, Lipase administration & dosage, Logistic Models, Male, Retrospective Studies, Ultrasonography, Amylases therapeutic use, Colon pathology, Cystic Fibrosis drug therapy, Endopeptidases therapeutic use, Lipase therapeutic use
- Abstract
Fibrotic strictures of the colon in association with colon-wall thickening on abdominal ultrasound have been described in children with cystic fibrosis. We determined the prevalence of colon-wall thickening in 99 children with cystic fibrosis (aged 6 months to 17 years, 45 male) and 38 healthy controls (aged 1 month to 39 years, 21 male). In controls, peristalsis was uniformly rapid and maximum wall thickness of the fully relaxed colon was 0.8 mm (median 0.6 mm). 24% of the patients had a colon-wall thickness of 1.5 mm or more (maximum 3.4 mm); 48% of children on high-strength pancreatin receiving more than 265 units/kg per day of protease had thickening of more than 1.5 mm compared with less than 12% of children on any other dose. Children on high-strength pancreatin were 5.2 (95% CI 1.2-21) times more likely to have colon-wall thickness of 1.5 mm or more than children taking less than 265 units/kg per day of protease in a standard-strength preparation. This risk rose to 10 (2.5-39) times more likely if laxatives were taken with a high-strength preparation. Lipase or amylase intake, age, sex, genotype, and other medical or drug history were not significantly associated with colon-wall thickening. 1 child required a hemicolectomy for bowel obstruction and another developed haemorrhagic colitis. In 17 children, 2 months after stopping high-strength, high protease, preparations and efforts to reduce enzyme intake, colon-wall thickness had regressed from a median 2.0 mm (range 1.0-3.4) to 1.8 mm (0.9-2.7) (p = 0.005). The use of high-strength pancreatin preparations in conjunction with a high protease intake probably causes thickening of the wall of the colon.
- Published
- 1995
- Full Text
- View/download PDF
27. High-strength pancreatic enzyme supplements and large-bowel stricture in cystic fibrosis.
- Author
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Oades PJ, Bush A, Ong PS, and Brereton RJ
- Subjects
- Cecum pathology, Child, Preschool, Colon pathology, Constriction, Pathologic chemically induced, Drug Combinations, Fibrosis chemically induced, Humans, Male, Amylases adverse effects, Bromelains adverse effects, Cystic Fibrosis complications, Intestinal Obstruction etiology, Lipase adverse effects, Trypsin adverse effects
- Published
- 1994
28. Prediction of hypoxaemia at high altitude in children with cystic fibrosis.
- Author
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Oades PJ, Buchdahl RM, and Bush A
- Subjects
- Acute Disease, Adolescent, Aircraft, Child, Cystic Fibrosis blood, Cystic Fibrosis physiopathology, Female, Humans, Lung physiopathology, Male, Oxygen blood, Respiratory Function Tests methods, Altitude, Cystic Fibrosis complications, Hypoxia etiology
- Abstract
Objective: To assess the usefulness of a hypoxic challenge in a laboratory at sea level in predicting acute desaturation at altitude in children with lung disease., Design: Comparison of responses to hypoxic challenge in different settings., Subjects: 22 children (12 boys) aged 11 to 16 years with cystic fibrosis in whom the mean forced expiratory volume in one second was 64% (range 24-100%)., Setting: Lung function laboratory, the Alps, and aboard commercial jet aircraft., Main Outcome Measures: Spirometric lung function at sea level and finger probe oximetry with air and 15% oxygen. Oximetry during high altitude flight and on a mountain at altitude of 1800 m., Results: Significant desaturation (range 0 to 12%) occurred with all hypoxic challenges (P < 0.002). The best predictor of hypoxic response from a single reading was the laboratory test (r2 = 76% for flight and r2 = 47% for mountain altitude), but the mean errors of prediction were not clinically significantly different. In six children who showed the greatest desaturation the laboratory test overestimated desaturation, but other predictors underestimated desaturation in three by up to 5%., Conclusions: The laboratory hypoxic challenge directly predicted the worst case of desaturation during flight and at equivalent high altitude. Spirometry and baseline oxygen saturations may underestimate individual hypoxic response. The test may have wider applications to other patients with stable chronic lung diseases, particularly in determining who needs supplementary oxygen during air travel and who should be advised against holidays at high altitude.
- Published
- 1994
- Full Text
- View/download PDF
29. The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo.
- Author
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Bolton VN, Oades PJ, and Johnson MH
- Subjects
- Amanitins pharmacology, Animals, Cell Division, Electrophoresis, Polyacrylamide Gel, Mice, Mice, Inbred Strains, Peptide Biosynthesis, Protein Biosynthesis, Time Factors, Cleavage Stage, Ovum, DNA Replication, Transcription, Genetic
- Abstract
The 2-cell stage of mouse embryogenesis is characterized by two phases of alpha-amanitin-sensitive polypeptide synthetic activity, which appear to mark the first major expression of the embryonic genome, as assessed by examination of in vitro translates of mRNA. Using populations of embryos synchronized to the first cleavage division, we have established that DNA replication takes place over the period 1 to 5.5 h after the first cleavage division; the two bursts of putative transcription take place before and immediately after DNA replication, and the translation products are detectable in each case within 3-4 h. In addition, we have shown that suppression of cytokinesis and the second round of DNA replication does not affect synthesis of the alpha-amanitin-sensitive polypeptides, and that neither DNA replication nor the loss of maternal mRNA that take place during the 2-cell stage are dependent upon synthesis of the alpha-amanitin-sensitive polypeptides.
- Published
- 1984
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