1. High-Performance Acellular Tissue Scaffold Combined with Hydrogel Polymers for Regenerative Medicine
- Author
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Dai Hyun Kim, Jae Ryun Ryu, Neon-Cheol Jung, Soo Hong Seo, Hyun Jung Kim, Gi Hoon Son, Mohammed R. Shaker, Eunsoo Lee, Woong Sun, Ki Won Lee, Im Joo Rhyu, Youngshik Choe, Min Seok Ham, and Hyun Kim
- Subjects
Decellularization ,Chemistry ,Regeneration (biology) ,0206 medical engineering ,Biomedical Engineering ,Soft tissue ,02 engineering and technology ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Regenerative medicine ,Biomaterials ,Extracellular matrix ,Tissue engineering ,Tissue Decellularization ,Self-healing hydrogels ,0210 nano-technology ,Biomedical engineering - Abstract
Decellularization of tissues provides extracellular matrix (ECM) scaffolds for regeneration therapy and an experimental model to understand ECM and cellular interactions. However, decellularization often causes microstructure disintegration and reduction of physical strength, which greatly limits the use of this technique in soft organs or in applications that require maintenance of physical strength. Here, we present a new tissue decellularization procedure, namely CASPER (Clinically and Experimentally Applicable Acellular Tissue Scaffold Production for Tissue Engineering and Regenerative Medicine), which includes infusion and hydrogel polymerization steps prior to robust chemical decellularization treatments. Polymerized hydrogels serve to prevent excessive damage to the ECM while maintaining the sophisticated structures and biological activities of ECM components in various organs, including soft tissues such as brains and embryos. CASPERized tissues were successfully recellularized to stimulate a tissue-regeneration-like process after implantation without signs of pathological inflammation or fibrosis in vivo, suggesting that CASPERized tissues can be used for monitoring cell-ECM interactions and for surrogate organ transplantation.
- Published
- 2019