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Correction: Corrigendum: ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies.
- Subjects :
- Electrophoresis
Male
0301 basic medicine
Tissue Fixation
Operations research
Polymers
Computer science
Xenopus
Article
Diffusion
Rats, Sprague-Dawley
Fixatives
Mice
03 medical and health sciences
Imaging, Three-Dimensional
Formaldehyde
Pressure
Animals
Humans
Zebrafish
Acrylamide
Histocytological Preparation Techniques
Multidisciplinary
Information retrieval
Tissue clearing
Brain
Hydrogels
Corrigenda
Rats
Mice, Inbred C57BL
030104 developmental biology
Microscopy, Fluorescence
Rabbits
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....26fcfe28cb44a2e84cbb6fd626163c60