Back to Search
Start Over
Enhanced electro-mediated gene delivery using carrier genes
- Source :
- Bioelectrochemistry. 78:186-190
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Low transfection and expression of target genes has been a problem in gene delivery for clinical therapy. In this study, we present a method for enhancing the transfection efficiency of target genes by electroporation using carrier genes. To evaluate the transfection efficiency, we transfected HeLa cells with luciferase genes (pGL3-control) and then measured the luciferase activity. In our experiments, the prokaryotic expression vector pCR2.1 was used as a carrier gene in the electro-mediated gene delivery. The result shows that the luciferase gene can be effectively transferred into the cell membrane with the aid of carrier genes. In the presence of carrier genes, luciferase activities increased two- to three-fold compared with that in the absence of carrier genes. We also investigated the effect of the weight ratio of luciferase genes to carrier genes on transfection efficiency and found no significant relationship between them. Consequently, we believe that carrier genes are potentially beneficial for promoting transfection and expression of target genes in biological applications.
- Subjects :
- animal structures
viruses
Genetic Vectors
Biophysics
Gene delivery
Transfection
HeLa
Cell membrane
Electrochemistry
medicine
Humans
Luciferase
Vector (molecular biology)
Physical and Theoretical Chemistry
Luciferases
Gene
biology
Electroporation
fungi
General Medicine
biology.organism_classification
Molecular biology
medicine.anatomical_structure
embryonic structures
HeLa Cells
Subjects
Details
- ISSN :
- 15675394
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Bioelectrochemistry
- Accession number :
- edsair.doi.dedup.....c3374375927665d0dec3b887d7a47188