35 results on '"Minhua Rong"'
Search Results
2. An Encapsulation of Gene Signatures for Hepatocellular Carcinoma, MicroRNA-132 Predicted Target Genes and the Corresponding Overlaps.
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Xin Zhang, Wei Tang, Gang Chen, Fanghui Ren, Haiwei Liang, Yiwu Dang, and Minhua Rong
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Medicine ,Science - Abstract
Previous studies have demonstrated that microRNA-132 plays a vital part in and is actively associated with several cancers, with its tumor-suppressive role in hepatocellular carcinoma confirmed. The current study employed multiple bioinformatics techniques to establish gene signatures for hepatocellular carcinoma, microRNA-132 predicted target genes and the corresponding overlaps.Various assays were performed to explore the role and cellular functions of miR-132 in HCC and a successive panel of tasks was completed, including NLP analysis, miR-132 target genes prediction, comprehensive analyses (gene ontology analysis, pathway analysis, network analysis and connectivity analysis), and analytical integration. Later, HCC-related and miR-132-related potential targets, pathways, networks and highlighted hub genes were revealed as well as those of the overlapped section.MiR-132 was effective in both impeding cell growth and boosting apoptosis in HCC cell lines. A total of fifty-nine genes were obtained from the analytical integration, which were considered to be both HCC- and miR-132-related. Moreover, four specific pathways were unveiled in the network analysis of the overlaps, i.e. adherens junction, VEGF signaling pathway, neurotrophin signaling pathway, and MAPK signaling pathway.The tumor-suppressive role of miR-132 in HCC has been further confirmed by in vitro experiments. Gene signatures in the study identified the potential molecular mechanisms of HCC, miR-132 and their established associations, which might be effective for diagnosis, individualized treatments and prognosis of HCC patients. However, combined detections of miR-132 with other bio-indicators in clinical practice and further in vitro experiments are needed.
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- 2016
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3. Underexpression of miR-34a in hepatocellular carcinoma and its contribution towards enhancement of proliferating inhibitory effects of agents targeting c-MET.
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Yiwu Dang, Dianzhong Luo, Minhua Rong, and Gang Chen
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Medicine ,Science - Abstract
Aberrant expression of microRNA-34a (miR-34a) has been reported to be involved in the tumorigenesis and progression of various classes of malignancies. However, its role in hepatocellular carcinoma (HCC) has not been completely clarified. In the current study, we have investigated the clinical significance and the in vitro contribution of miR-34a on biological functions of human HCCs. miR-34a expression in eighty-three cases of HCC formalin-fixed paraffin-embedded (FFPE) tissues decreased significantly compared to that in the adjacent liver tissues (P
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- 2013
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4. Clinical Implication of E2F Transcription Factor 1 in Hepatocellular Carcinoma Tissues
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Hui-Ping Lu, Rong-Quan He, Qiu-Yu Pang, Wang-Yang Ye, Yu-Yan Pang, Gang Chen, Li-Hua Yang, Shang-Ling Pan, Huayu Wu, Xian-Guo Zhou, Yi-Wu Dang, Minhua Rong, Jian-Di Li, and Wei-Ying He
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Pharmacology ,endocrine system ,Cancer Research ,Tissue microarray ,Cancer ,General Medicine ,Cell cycle ,Biology ,medicine.disease ,digestive system diseases ,Oncology ,Hepatocellular carcinoma ,Cancer research ,medicine ,Immunohistochemistry ,E2F1 ,Radiology, Nuclear Medicine and imaging ,biological phenomena, cell phenomena, and immunity ,E2F ,Transcription factor - Abstract
Background: To date, the clinical management of advanced hepatocellular carcinoma (HCC) patients remains tough and the mechanisms of E2F transcription factor 1 (E2F1) underlying HCC are obscure. Materials and Methods: Our study integrated datasets mined from several public databases to comprehensively understand the deregulated expression status of E2F1. Tissue microarrays and immunohistochemistry staining was used to validate E2F1 expression level. The prognostic value of E2F1 was assessed. In-depth subgroup analyses were implemented to compare the differentially expressed levels of E2F1 in HCC patients with various tumor stages. Functional enrichments were used to address the predominant targets of E2F1 and shedding light on their potential roles in HCC. Results: We confirmed the elevated expression of E2F1 in HCC. Subgroup analyses indicated that elevated E2F1 level was independent of various stages in HCC. E2F1 possessed moderate discriminatory capability in differentiating HCC patients from non-HCC controls. Elevated E2F1 correlated with Asian race, tumor classification, neoplasm histologic grade, eastern cancer oncology group, and plasma AFP levels. Furthermore, high E2F1 correlated with poor survival condition and pooled HR signified E2F1 as a risk factor for HCC. Enrichment analysis of differentially expressed genes, coexpressed genes, and putative targets of E2F1 emphasized the importance of cell cycle pathway, where CCNE1 and CCNA2 served as hub genes. Conclusions: We confirmed the upregulation of E2F1 and explored the prognostic value of E2F1 in HCC patients. Two putative targeted genes (CCNE1 and CCNA2) of E2F1 were identified for their potential roles in regulating cell cycle and promote antiapoptotic activity in HCC patients.
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- 2021
5. Effect of CELSR3 on the Cell Cycle and Apoptosis of Hepatocellular Carcinoma Cells: Erratum
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Zucheng Xie, Yiwu Dang, Huayu Wu, Rongquan He, Jie Ma, Zhigang Peng, Minhua Rong, Zhekun Li, Jiapeng Yang, Yizhao Jiang, Gang Chen, and Lihua Yang
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Oncology - Published
- 2022
6. Corrigendum to 'Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells'
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Gang Chen, Su-Ning Huang, Yi-Wu Dang, Rong-Quan He, and Minhua Rong
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Carcinoma, Hepatocellular ,Cell Survival ,Cetuximab ,Antineoplastic Agents ,Apoptosis ,Antibodies, Monoclonal, Humanized ,General Biochemistry, Genetics and Molecular Biology ,Text mining ,medicine ,Humans ,General Immunology and Microbiology ,business.industry ,Liver Neoplasms ,Drug Synergism ,General Medicine ,Hep G2 Cells ,medicine.disease ,MicroRNAs ,Treatment Outcome ,Hepatocellular carcinoma ,Cancer research ,Medicine ,business ,Corrigendum ,medicine.drug - Abstract
Previously, we found that the expression of microRNA-146a (miR-146a) was downregulated in hepatocellular carcinoma (HCC) formalin-fixed paraffin-embedded (FFPE) tissues compared to the adjacent noncancerous hepatic tissues. In the current study, we have explored the in vitro effect of miR-146a on the malignant phenotypes of HCC cells. MiR-146a mimic could suppress cell growth and increase cellular apoptosis in HCC cell lines HepG2, HepB3, and SNU449, as assessed by spectrophotometry, fluorimetry, and fluorescence microscopy, respectively. Furthermore, western blot showed that miR-146a mimic downregulated EGFR, ERK1/2, and stat5 signalings. These effects were less potent compared to that of a siRNA targeting EGFR, a known target gene of miR-146a. Moreover, miR-146a mimic could enhance the cell growth inhibition and apoptosis induction impact of various EGFR targeting agents. The most potent combination was miR-146a mimic with cetuximab, presenting a synergistic effect. In conclusion, miR-146a plays a vital role in the cell growth and apoptosis of HCC cells and inducing miR-146a level might be a critical targeted molecular therapy strategy for HCC.
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- 2020
7. The clinical significance of interleukin 24 and its potential molecular mechanism in laryngeal squamous cell carcinoma
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Xiao-Yi Chen, Gang Chen, Yao Liang, Guo-Sheng Li, Xiao-Zhun Tang, Xiao-Guohui Zhang, Yi-Wu Dang, Yu-Xuan Yao, Su-Ning Huang, Ming-Xuan Li, Xian-Guo Zhou, Zhi-Guang Huang, and Minhua Rong
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Male ,Cancer Research ,MMP3 ,Integrins ,MMP1 ,Microarray ,Biology ,Transforming Growth Factor beta1 ,03 medical and health sciences ,0302 clinical medicine ,Interleukin 24 ,Plasminogen Activator Inhibitor 1 ,Genetics ,Biomarkers, Tumor ,Humans ,Gene Regulatory Networks ,Protein Interaction Maps ,KEGG ,Gene ,030304 developmental biology ,0303 health sciences ,Squamous Cell Carcinoma of Head and Neck ,Interleukins ,Computational Biology ,Granulocyte-Macrophage Colony-Stimulating Factor ,General Medicine ,Middle Aged ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Carcinoma, Squamous Cell ,Immunohistochemistry ,Female ,Matrix Metalloproteinase 3 ,Matrix Metalloproteinase 1 ,Extracellular matrix organization ,Signal Transduction - Abstract
Interleukin 24 (IL24) has been documented to be highly expressed in several cancers, but its role in laryngeal squamous cell carcinoma (LSCC) remains unclarified. In this study, to reveal the function and its clinical significance of IL24 in LSCC, multiple detecting methods were used comprehensively. IL24 protein expression was remarkably higher in LSCC (n= 49) than non-cancerous laryngeal controls (n= 26) as detected by in-house immunohistochemistry. Meanwhile, the IL24 mRNA expression was also evaluated based on high throughput data from Gene Expression Omnibus, The Cancer Genome Atlas, ArrayExpress and Oncomine databases. Consistently with the protein level, IL24 mRNA expression level was also predominantly upregulated in LSCC (n= 172) compared to non-cancerous laryngeal tissues (n= 81) with the standard mean difference (SMD) being 1.25 and the area under the curve (AUC) of the summary receiver operating characteristic (sROC) being 0.89 (95% CI = 0.86–0.92). Furthermore, the related genes of IL24 and the differentially expressed genes (DEGs) of LSCC were intersected and sent for Gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and the protein-protein interaction (PPI) analyses. In the GO annotation, the top terms of biological process (BP), cellular component (CC) and molecular function (MF) were extracellular matrix organization, extracellular matrix, cytokine activity, respectively. The top pathway of KEGG was ECM-receptor interaction. The PPI networks indicated the top hub genes of IL24-related genes in LSCC were SERPINE1, TGFB1, MMP1, MMP3, CSF2, and ITGA5. In conclusion, upregulating expression of IL24 may enhance the occurrence of LSCC, which owns prospect diagnostic ability and therapeutic significance in LSCC.
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- 2020
8. Identification of an Immune Score-Based Gene Panel with Prognostic Power for Oral Squamous Cell Carcinoma
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Gang Chen, Xiao-Yi Chen, Xiao-Zhun Tang, Ping Li, Su-Ning Huang, Guo-Sheng Li, Yao Liang, Ming-Xuan Li, Yi-Wu Dang, Xiao-Guohui Zhang, Yu-Xuan Yao, Minhua Rong, Jing Li, Zhi-Guang Huang, and Xian-Guo Zhou
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Oncology ,Male ,medicine.medical_specialty ,Stromal cell ,Support Vector Machine ,Corneal Stroma ,030204 cardiovascular system & hematology ,Risk Assessment ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Lymphocytes, Tumor-Infiltrating ,Internal medicine ,Databases, Genetic ,medicine ,Tumor Microenvironment ,Humans ,Basal cell ,Protein Interaction Maps ,RNA, Messenger ,RNA-Seq ,Receptors, Cytokine ,Survival rate ,Proportional Hazards Models ,Tumor microenvironment ,Immunity, Cellular ,Receiver operating characteristic ,Cell adhesion molecule ,business.industry ,Squamous Cell Carcinoma of Head and Neck ,Sequence Analysis, RNA ,Gene Expression Profiling ,Cancer ,General Medicine ,medicine.disease ,Prognosis ,Microarray Analysis ,stomatognathic diseases ,ROC Curve ,030220 oncology & carcinogenesis ,Immune System ,Multivariate Analysis ,Database Analysis ,Cytokines ,Female ,Mouth Neoplasms ,business ,Transcriptome - Abstract
BACKGROUND Oral squamous cell carcinoma (OSCC) is the sixth most prevalent cancer worldwide, with low 5-year survival rate. To identify novel prognostic markers for OSCC and determine the immune and stromal landscape of OSCC, a risk signature for OSCC patients was constructed in this study. MATERIAL AND METHODS Immune and stromal scores for OSCC samples from the Genomic Data Commons Data Portal were computed to delineate the tumor microenvironment landscape of oral cancer based on the Estimation of STromal and Immune cells in MAlignant Tumours using Expression data algorithm. An immune score-based risk signature was constructed by combining random forest and support vector machine methods. Correlation analysis of risk signature gene expression and immune cell infiltration was conducted, and the distinguishing power of individual signature genes was evaluated by analyzing receiver operating characteristics (ROC) curves. Differentially enriched pathways between high and low risk groups were investigated via gene set variation analysis. ROC curves were plotted for signature genes to examine their ability to distinguish the recurrence and survival status of OSCC patients from GSE84846. RESULTS An immune score-related risk signature composed of ARMH1, F2RL2, AC004687.1, COL6A5, AC008750.1, RAB19, CRLF2, GRIP2, and FAM162B performed well in the prognostic stratification of OSCC patients and could effectively distinguish their survival status. Lists of pathways, including cytokine-cytokine receptor interaction and cell adhesion molecules displayed remarkable differential enrichment between high and low risk OSCC patients. CONCLUSIONS An immune score-based risk signature constructed presently may be useful to decide appropriate treatment options for individual OSCC patients.
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- 2020
9. Clinical value and potential mechanisms of COL8A1 upregulation in breast cancer: a comprehensive analysis
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Wei Peng, Wen-Bin Dai, Jing-Jing Zeng, Xiao-Ping Zou, Wei Tang, Deng Tang, Gang Chen, Minhua Rong, Zhong-Qing Tang, Ying Li, Jian-Di Li, and Zhen-Bo Feng
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Oncology ,Cancer Research ,medicine.medical_specialty ,Alpha (ethology) ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Downregulation and upregulation ,Internal medicine ,Genetics ,medicine ,COL8A1 ,lcsh:QH573-671 ,Gene ,030304 developmental biology ,0303 health sciences ,business.industry ,lcsh:Cytology ,Cancer ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,MRNA Sequencing ,030220 oncology & carcinogenesis ,Immunohistochemistry ,Mechanism ,DNA microarray ,business ,Primary Research ,Immunohistochemistry staining - Abstract
Background The situation faced by breast cancer patients, especially those with triple-negative breast cancer, is still grave. More effective therapeutic targets are needed to optimize the clinical management of breast cancer. Although collagen type VIII alpha 1 chain (COL8A1) has been shown to be downregulated in BRIP1-knockdown breast cancer cells, its clinical role in breast cancer remains unknown. Methods Gene microarrays and mRNA sequencing data were downloaded and integrated into larger matrices based on various platforms. Therefore, this is a multi-centered study, which contains 5048 breast cancer patients and 1161 controls. COL8A1 mRNA expression in breast cancer was compared between molecular subtypes. In-house immunohistochemistry staining was used to evaluate the protein expression of COL8A1 in breast cancer. A diagnostic test was performed to assess its clinical value. Furthermore, based on differentially expressed genes (DEGs) and co-expressed genes (CEGs) positively related to COL8A1, functional enrichment analyses were performed to explore the biological function and potential molecular mechanisms of COL8A1 underlying breast cancer. Results COL8A1 expression was higher in breast cancer patients than in control samples (standardized mean difference = 0.79; 95% confidence interval [CI] 0.55–1.03). Elevated expression was detected in various molecular subtypes of breast cancer. An area under a summary receiver operating characteristic curve of 0.80 (95% CI 0.76–0.83) with sensitivity of 0.77 (95% CI 0.69–0.83) and specificity of 0.70 (95% CI 0.61–0.78) showed moderate capacity of COL8A1 in distinguishing breast cancer patients from control samples. Worse overall survival was found in the higher than in the lower COL8A1 expression groups. Intersected DEGs and CEGs positively related to COL8A1 were significantly clustered in the proteoglycans in cancer and ECM-receptor interaction pathways. Conclusions Elevated COL8A1 may promote the migration of breast cancer by mediating the ECM-receptor interaction and synergistically interplaying with DEGs and its positively related CEGs independently of molecular subtypes. Several genes clustered in the proteoglycans in cancer pathway are potential targets for developing effective agents for triple-negative breast cancer.
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- 2020
10. Erratum to 'The role of upregulated miR-375 expression in breast cancer: An in vitro and in silico study' [Pathol. Res. Pract. 216 (January (1)) (2020) 152754]
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Gang Chen, Wei Tang, Zhen-Bo Feng, Su-Ning Huang, Chao-Hua Mo, Minhua Rong, Qi Shi, Jian-Di Li, Jing-Jing Zeng, Dan-Dan Xiong, Wen-Ya Pan, and Guo-Sheng Li
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2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,In silico ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Cell Biology ,medicine.disease ,In vitro ,Pathology and Forensic Medicine ,Breast cancer ,Downregulation and upregulation ,Mir-375 ,Cancer research ,medicine ,business - Published
- 2020
11. Identification of prognostic splicing factors and exploration of their potential regulatory mechanisms in pancreatic adenocarcinoma
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Mei-wei Li, Jia-shuo Zheng, Dan-Ming Wei, Hui-liu Peng, Minhua Rong, Ming-hui Chen, Yue-qi Huang, Ying Guan, Su-Ning Huang, Zhan-Hui Zhu, Ying-mei Li, Ming-Xuan Li, Xiao-lin Wei, Xiao-ju Wu, Hui-ping Bu, and Guo-Sheng Li
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musculoskeletal diseases ,Bioinformatics ,RNA-sequencing ,lcsh:Medicine ,Disease ,Computational biology ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Splicing factors ,03 medical and health sciences ,0302 clinical medicine ,Lasso (statistics) ,Pancreatic cancer ,Evidence Based Medicine ,medicine ,Overall survival ,Clinical significance ,030304 developmental biology ,0303 health sciences ,General Neuroscience ,lcsh:R ,Alternative splicing ,General Medicine ,medicine.disease ,Oncology ,030220 oncology & carcinogenesis ,RNA splicing ,Adenocarcinoma ,General Agricultural and Biological Sciences ,Medical Genetics ,Pancreatic adenocarcinoma - Abstract
Pancreatic adenocarcinoma (PAAD), the most common subtype of pancreatic cancer, is a highly lethal disease. In this study, we integrated the expression profiles of splicing factors (SFs) of PAAD from RNA-sequencing data to provide a comprehensive view of the clinical significance of SFs. A prognostic index (PI) based on SFs was developed using the least absolute shrinkage and selection operator (LASSO) COX analysis. The PI exhibited excellent performance in predicting the status of overall survival of PAAD patients. We also used the percent spliced in (PSI) value obtained from SpliceSeq software to quantify different types of alternative splicing (AS). The prognostic value of AS events was explored using univariate COX and LASSO COX analyses; AS-based PIs were also proposed. The integration of prognosis-associated SFs and AS events suggested the potential regulatory mechanisms of splicing processes in PAAD. This study defined the markedly clinical significance of SFs and provided novel insight into their potential regulatory mechanisms.
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- 2019
12. Downregulation of microRNA-132 indicates progression in hepatocellular carcinoma
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Ting-Qing Gan, Gang Chen, Minhua Rong, Wei Tang, Yihuan Luo, Ruishan Li, Rong-Quan He, and Xin Zhang
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0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Pathology ,microRNA-132 ,Biology ,medicine.disease_cause ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,Internal medicine ,microRNA ,medicine ,reverse transcription-quantitative polymerase chain reaction ,Hepatitis B virus ,Receiver operating characteristic ,Oncogene ,Articles ,hepatocellular carcinoma ,General Medicine ,Cell cycle ,Hepatitis B ,medicine.disease ,Molecular medicine ,030104 developmental biology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,progression - Abstract
Although miR-132 has been studied in various human tumors, few studies have investigated the role of miR-132 in hepatocellular carcinoma (HCC). The present study aimed to evaluate the associations between miR-132 and clinicopathological parameters, including recurrence, in patients with HCC. Reverse transcription-quantitative polymerase chain reaction analysis was used to detect the expression levels of miR-132 in 95 cases of HCC and their corresponding non-cancerous liver tissues. Th e associations between miR-132 expression levels and clinicopathological characteristics, including recurrence, were investigated in patients with HCC. miR-132 expression levels were significantly reduced in HCC tissues, as compared with adjacent non-cancerous tissues (1.9245±0.7564 vs. 2.7326±1.1475; P
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- 2016
13. Clinicopathological value and underlying molecular mechanism of annexin A2 in 992 cases of thyroid carcinoma
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Su-Ning Huang, Dan-Ming Wei, Gang Chen, Yu-Yan Pang, Wen-wen Xing, Minhua Rong, Xiao-jiao Li, Yong-Ying Qin, Xiao-Zhun Tang, and Yun He
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0301 basic medicine ,Microarray ,Thyroid Gland ,Biology ,Biochemistry ,Thyroid carcinoma ,03 medical and health sciences ,0302 clinical medicine ,Structural Biology ,microRNA ,Gene expression ,medicine ,Humans ,Thyroid Neoplasms ,Annexin A2 ,Reporter gene ,Sequence Analysis, RNA ,Microarray analysis techniques ,Organic Chemistry ,Thyroid ,Up-Regulation ,MicroRNAs ,Computational Mathematics ,Gene Ontology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research - Abstract
Thyroid carcinoma (THCA) is one of the most frequent endocrine cancers and has increasing morbidity. Annexin A2 (ANXA2) has been found to be highly expressed in various cancers; however, its expression level and potential mechanism in THCA remain unknown. This study investigated the clinicopathological value and primary molecular machinery of ANXA2 in THCA.Public RNA-sequencing and microarray data were obtained and analyzed with ANXA2 expression in THCA and corresponding non-cancerous thyroid tissue. A Pearson correlation coefficient calculation was used for the acquisition of ANXA2 coexpressed genes, while edgR, limma, and Robust Rank Aggregation were employed for differentially expressed gene (DEG) in THCA. The probable mechanism of ANXA2 in THCA was predicted by gene ontology and pathway enrichment. A dual-luciferase reporter assay was employed to confirm the targeting relationships between ANXA2 and its predicted microRNA (miRNA).Expression of ANXA2 was significantly upregulated in THCA tissues with a summarized standardized mean difference of 1.09 (P0.0001) based on 992 THCA cases and 589 cases of normal thyroid tissue. Expression of ANXA2 was related to pathologic stage. Subsequently, 1442 genes were obtained when overlapping 4542 ANXA2 coexpressed genes with 2248 DEGs in THCA; these genes were mostly enriched in pathways of extracellular matrix-receptor interaction, cell adhesion molecules, and complement and coagulation cascades. MiR-23b-3p was confirmed to target ANXA2 by dual-luciferase reporter assay.Upregulated expression of ANXA2 may promote the malignant biological behavior of THCA by affecting the involving pathways or being targeted by miR-23b-3p.
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- 2020
14. The role of upregulated miR-375 expression in breast cancer: An in vitro and in silico study
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Gang Chen, Chao-Hua Mo, Qi Shi, Wei Tang, Wen-Ya Pan, Jian-Di Li, Zhen-Bo Feng, Su-Ning Huang, Minhua Rong, Jing-Jing Zeng, Dan-Dan Xiong, and Guo-Sheng Li
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Transcriptional Activation ,0301 basic medicine ,Breast Neoplasms ,Somatic stem cell division ,Biology ,medicine.disease_cause ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Mir-375 ,microRNA ,Biomarkers, Tumor ,medicine ,Humans ,Computer Simulation ,Protein Interaction Maps ,KEGG ,MicroRNA sequencing ,Gene Expression Profiling ,Cell Biology ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,ROC Curve ,030220 oncology & carcinogenesis ,Cancer research ,Female ,DNA microarray ,Carcinogenesis - Abstract
Breast cancer (BC) is the most common cancer worldwide. However, the expression and potential mechanism of miR-375 in BC are still controversial. We first collected microRNA chips and microRNA sequencing data from multiple databases for analyzing the expression level of miR-375, and further exploring the target genes and underlying molecular mechanism in BC. miR-375 in BC was predominantly overexpressed compared with that in normal breast tissues (pooled standard mean difference [SMD] = 0.49; 95 % confidence interval [CI]: 0.24-0.73, p < 0.0001). Meanwhile, the overall pooled area under the curve (AUC) in the summary receiver operating characteristic (SROC) of miR-375 was 0.83 (95 % CI = 0.79-0.86) based on 2928 cases of BC patients and 816 cases of controls, while the diagnostic positive likelihood ratio (DLR) positive and the DLR negative value were 3.90 (95 % CI = 2.46-6.19) and 0.39 (95 % CI = 0.28-0.54), respectively. The hazard ratios (HRs) were 1.29 (95 % CI = 1.04-1.6, P = 0.02) and 1.23 (95 % CI = 0.89-1.7, P = 0.22) for the cohorts of METABRIC and The Cancer Genome Atlas (TCGA). In vitro study demonstrated that miR-375 inhibitor could suppress the cell growth and induce apoptosis of BC cells. A total of 107 overlapping genes from microarrays after miR-375 transfection, the TCGA RNA sequencing, the microarrays of Affymetrix platform, and online predicting software were selected as the prospective targets of miR-375 in BC. Based on Gene Ontology (GO) enrichment analysis, the potential targets of miR-375 were notable for their somatic stem cell division, plasma membrane, and proline-rich region binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway examination demonstrated that the targets were associated with the pathways of prion diseases, proteoglycans in cancer, and focal adhesion. Then, 107 targets of miR-375 in BC were used to construct a protein-protein interaction (PPI) network. Finally, EGFR, PRKCA, PPARA, ADIPOQ, and ITSN1 were found to be the hub genes of miR-375. These targets showed negative correlations with miR-375 level. The upregulated miR-375 might play an essential part in the tumorigenesis and progression of BC.
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- 2020
15. Correlation between down-expression of miR-431 and clinicopathological significance in HCC tissues
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Yihuan Luo, Lin-jiang Pan, Gang Chen, Dian-Zhong Luo, Yi-Wu Dang, Minhua Rong, and Fanghui Ren
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Down-Regulation ,Gastroenterology ,Metastasis ,Cohort Studies ,Neoplasms, Multiple Primary ,Internal medicine ,medicine ,Carcinoma ,Humans ,Prospective Studies ,Stage (cooking) ,Prospective cohort study ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Receiver operating characteristic ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Liver Neoplasms ,MTDH ,General Medicine ,Hepatitis C, Chronic ,Middle Aged ,medicine.disease ,Tumor Burden ,Gene Expression Regulation, Neoplastic ,Reverse transcription polymerase chain reaction ,MicroRNAs ,Oncology ,Lymphatic Metastasis ,Biomarker (medicine) ,Female ,Lymph Nodes ,alpha-Fetoproteins ,business - Abstract
Researches have shown that miRNAs have been proposed as novel diagnostic biomarkers for classification and prognostic stratification of HCC. However, whether or not miR-431 contributes to the progression of HCC remains unknown. Therefore, we aimed to investigate the clinicopathological significance of miR-431 in HCC. MiR-431 expression in 95 HCC cases and corresponding adjacent non-cancerous tissues was evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, statistical analysis was performed to identify the correlations between expression of miR-431 and a variety of clinicopathological parameters and patient recurrence. The area under the receiver operating characteristic curve (AUC) was used to evaluate the accuracy of miR-431 as a biomarker for HCC diagnosis and prediction of disease deterioration. MiR-431 was markedly down-regulated in the HCC samples (1.1885 ± 0.75867) compared with corresponding adjacent tumor tissues (1.7957 ± 0.89333, P
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- 2015
16. Promising significance of the association of miR-204-5p expression with clinicopathological features of hepatocellular carcinoma
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Xin Zhang, Wei Tang, Yi-Wu Dang, Na Zhao, Minhua Rong, Ji Cao, Si-Min Pang, Zhong Tan, Wen-Liang Guo, and Yihuan Luo
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0301 basic medicine ,Adult ,Carcinoma, Hepatocellular ,Observational Study ,medicine.disease_cause ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma ,Biomarkers, Tumor ,Medicine ,Humans ,Clinical significance ,KEGG ,Neoplasm Metastasis ,Gene ,Survival analysis ,Aged ,Neoplasm Staging ,Aged, 80 and over ,business.industry ,Liver Neoplasms ,miR-204-5p ,qRT-PCR ,General Medicine ,hepatocellular carcinoma ,Middle Aged ,TCGA ,medicine.disease ,GEO ,Survival Analysis ,digestive system diseases ,pathway analysis ,MicroRNAs ,030104 developmental biology ,Liver ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer research ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Disease Progression ,business ,Carcinogenesis ,Research Article - Abstract
Supplemental Digital Content is available in the text, Decreased level of miR-204-5p has been documented in various malignancies. However, the expression and clinical significance of miR-204-5p in hepatocellular carcinoma has not been investigated. The aim of this study is to examine the relationship between miR-204-5p expression and clinicopathological features in hepatocellular carcinoma (HCC) as well as to predict the relevant signaling pathways. The miR-204-5p expression level was detected in HCC and in matched paraneoplastic liver from 95 formalin-fixed paraffin-embedded tissues by the real-time reverse transcription polymerized chain reaction (qRT-PCR). The association of miR-204-5p expression with clinicopathological features as well as the prognosis of HCC was examined. Public data portals including the Gene Expression Omnibus and The Cancer Genome Atlas were used to retrieve the HCC-related data in order to perform a comprehensive meta-analysis. Meanwhile, protein–protein interaction (PPI) and enrichment analyses were performed using predicted target genes. The relative expression of miR-204-5p was remarkably reduced in HCC than that in paraneoplastic hepatic tissues. In HCC, the miR-204-5p expression was downregulated in the metastasis, vasoinvasion, and advanced stage (III and IV) subgroups compared with their counterparts. Furthermore, the meta-analysis based on qRT-PCR data demonstrated that miR-204-5p was markedly downregulated in HCC with a standardized mean difference of –5.19 (P
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- 2017
17. The impact of atosiban on pregnancy outcomes in women undergoing in vitro fertilization-embryo transfer: A meta-analysis
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Qian-Yi Huang, Xiaomiao Lin, Mu-Jun Li, Gang Chen, Ai-Hua Lan, Minhua Rong, Xinggu Lin, and Rong-Quan He
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0301 basic medicine ,Embryology ,Pregnancy Rate ,medicine.medical_treatment ,Maternal Health ,lcsh:Medicine ,Miscarriage ,Database and Informatics Methods ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Vasotocin ,Pregnancy ,Medicine and Health Sciences ,Odds Ratio ,Database Searching ,lcsh:Science ,In Vitro Fertilization ,education.field_of_study ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,Ectopic pregnancy ,Obstetrics ,Obstetrics and Gynecology ,Embryo transfer ,Physical Sciences ,Female ,Pregnancy, Multiple ,Live Birth ,Statistics (Mathematics) ,medicine.drug ,Research Article ,medicine.medical_specialty ,Population ,Fertilization in Vitro ,Research and Analysis Methods ,03 medical and health sciences ,Hormone Antagonists ,medicine ,Humans ,Embryo Implantation ,Statistical Methods ,education ,Demography ,Gynecology ,In vitro fertilisation ,business.industry ,lcsh:R ,Embryos ,Biology and Life Sciences ,Atosiban ,Birth Rates ,medicine.disease ,Embryo Transfer ,Pregnancy Complications ,Abortion, Spontaneous ,Pregnancy rate ,030104 developmental biology ,Fertilization ,Case-Control Studies ,People and Places ,Women's Health ,lcsh:Q ,business ,Mathematics ,Meta-Analysis ,Developmental Biology - Abstract
Background Atosiban is administered to women undergoing in vitro fertilization-embryo transfer (IVF-ET) to improve pregnancy outcomes. However, the results of this treatment were controversial. We conducted this meta-analysis to investigate whether atosiban improves pregnancy outcomes in the women undergoing in vitro fertilization (IVF). Methods Databases of PubMed, EMBASE, Web of Science, China BioMedicine, and Google Scholar were systematically searched. Meta-analyses were performed to investigate whether atosiban improves pregnancy outcomes in the women undergoing IVF. Results Our results showed that atosiban was associated with higher implantation (OR = 1.63, 95% CI: 1.17–2.27; P = 0.004) and clinical pregnancy (OR = 1.84, 95% CI: 1.31–2.57; P < 0.001) rates. However, atosiban showed no significant association with the miscarriage, live birth, multiple pregnancy or ectopic pregnancy rates. When a further subgroup analysis was performed in the women undergoing repeated implantation failure (RIF), implantation (OR = 1.93, 95% CI: 1.45–2.57; P < 0.001), clinical pregnancy (OR = 2.48, 95% CI: 1.70–3.64; P
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- 2017
18. An Encapsulation of Gene Signatures for Hepatocellular Carcinoma, MicroRNA-132 Predicted Target Genes and the Corresponding Overlaps
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Gang Chen, Minhua Rong, Hai-Wei Liang, Fanghui Ren, Wei Tang, Xin Zhang, and Yi-Wu Dang
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0301 basic medicine ,Cell signaling ,lcsh:Medicine ,Signal transduction ,Bioinformatics ,Biochemistry ,Database and Informatics Methods ,0302 clinical medicine ,VEGF Signaling Pathway ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,Liver Diseases ,Liver Neoplasms ,Signaling cascades ,Transfection ,Genomics ,VEGF signaling ,Nucleic acids ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Network Analysis ,Research Article ,Cell biology ,Computer and Information Sciences ,MAPK signaling cascades ,Carcinoma, Hepatocellular ,Gene prediction ,Computational biology ,Gastroenterology and Hepatology ,Biology ,Research and Analysis Methods ,Carcinomas ,Adherens junction ,03 medical and health sciences ,Cell Line, Tumor ,microRNA ,Gastrointestinal Tumors ,medicine ,Genetics ,Humans ,Gene Prediction ,Molecular Biology Techniques ,Non-coding RNA ,Gene ,Molecular Biology ,Biology and life sciences ,Cell growth ,lcsh:R ,Cancers and Neoplasms ,Computational Biology ,Hepatocellular Carcinoma ,medicine.disease ,Genome Analysis ,Gene regulation ,MicroRNAs ,030104 developmental biology ,RNA ,lcsh:Q ,Gene expression - Abstract
Objectives Previous studies have demonstrated that microRNA-132 plays a vital part in and is actively associated with several cancers, with its tumor-suppressive role in hepatocellular carcinoma confirmed. The current study employed multiple bioinformatics techniques to establish gene signatures for hepatocellular carcinoma, microRNA-132 predicted target genes and the corresponding overlaps. Methods Various assays were performed to explore the role and cellular functions of miR-132 in HCC and a successive panel of tasks was completed, including NLP analysis, miR-132 target genes prediction, comprehensive analyses (gene ontology analysis, pathway analysis, network analysis and connectivity analysis), and analytical integration. Later, HCC-related and miR-132-related potential targets, pathways, networks and highlighted hub genes were revealed as well as those of the overlapped section. Results MiR-132 was effective in both impeding cell growth and boosting apoptosis in HCC cell lines. A total of fifty-nine genes were obtained from the analytical integration, which were considered to be both HCC- and miR-132-related. Moreover, four specific pathways were unveiled in the network analysis of the overlaps, i.e. adherens junction, VEGF signaling pathway, neurotrophin signaling pathway, and MAPK signaling pathway. Conclusions The tumor-suppressive role of miR-132 in HCC has been further confirmed by in vitro experiments. Gene signatures in the study identified the potential molecular mechanisms of HCC, miR-132 and their established associations, which might be effective for diagnosis, individualized treatments and prognosis of HCC patients. However, combined detections of miR-132 with other bio-indicators in clinical practice and further in vitro experiments are needed.
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- 2016
19. TNFRSF6B neutralization antibody inhibits proliferation and induces apoptosis in hepatocellular carcinoma cell
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Minhua Rong, Gang Chen, and Dian-Zhong Luo
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Adult ,Male ,Carcinoma, Hepatocellular ,Apoptosis ,Cell Separation ,DNA Fragmentation ,Pathology and Forensic Medicine ,Flow cytometry ,Microscopy, Electron, Transmission ,Cell Line, Tumor ,In Situ Nick-End Labeling ,medicine ,Humans ,RNA, Messenger ,Aged ,Cell Proliferation ,Neoplasm Staging ,Retrospective Studies ,Aged, 80 and over ,medicine.diagnostic_test ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Cell growth ,Chemistry ,Liver Neoplasms ,Receptors, Tumor Necrosis Factor, Member 6b ,Cell Biology ,Middle Aged ,Cell cycle ,Flow Cytometry ,medicine.disease ,Antibodies, Neutralizing ,Immunohistochemistry ,digestive system diseases ,Cell culture ,Hepatocellular carcinoma ,Cancer research ,biology.protein ,Female ,Tumor necrosis factor alpha ,Antibody - Abstract
The tumor necrosis factor receptor super-family member 6b (TNFRSF6B) is over-expressed in various human cancers, but its function in hepatocellular carcinoma (HCC) remains uncertain. The aim of the study was to investigate the relationship between TNFRSF6B expression and apoptosis in HCC and the effect of anti-TNFRSF6B neutralization monoclonal antibody (McAb) on HCC cells. TNFRSF6B mRNA and protein expression were compared with apoptosis in 78 cases of HCC. Proliferation, cell cycle, apoptosis, and migration ability of liver cancer cells co-cultured with anti-TNFRSF6B McAb were also detected. TNFRSF6B mRNA and protein expression in the tumor tissues negatively correlated with apoptosis. Cell proliferation was decreased, cell cycle was arrested in G1/S-phase, apoptosis was increased, and migration ability was inhibited by anti-TNFRSF6B McAb in vitro. Anti-TNFRSF6B McAb could be useful to suppress proliferation and induce apoptosis in HCC. Thus, TNFRSF6B might be a critical, targeted therapy strategy for HCC.
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- 2010
20. Prognostic significance of MiR-34a in solid tumors: a systemic review and meta-analysis with 4030 patients
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Fanghui, Ren, Xin, Zhang, Haiwei, Liang, Dianzhong, Luo, Minhua, Rong, Yiwu, Dang, and Gang, Chen
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Original Article - Abstract
Purpose: The aim of the present meta-analysis and systematic review was to explore the association between the expression of miR-34a and prognosis in solid tumor. Methods: PubMed, Google Scholar, Web of Science and NCBI databases were used to search studies to evaluate the effect of miR-34a expression on clinical outcomes, including overall survival (OS), recurrence-free survival (RFS), disease-free survival (DFS), progression-free survival (PFS) and event-free survival (EFS) in solid tumor. The pooled random effect models were performed to calculate pooled hazard ratio (HR), 95% confidence interval (CI) to assess the association. Results: Twenty-three eligible studies with 4030 patients were included in this meta-analysis. It was confirmed that increased expression of miR-34a was in relevant with better DFS/RFS/PFS/EFS, which was identified with both univariate and multivariate models (univariate model: HR = 0.62, 95% CI: 0.42-0.92, P = 0.019; multivariate model: HR = 0.55, 95% CI: 0.34-0.88, P = 0.013). Furthermore, in the analysis of relationship between miR-34a and DFS/RFS/PFS/EFS, the results remained similar when excluding the studies contributed to the heterogeneity (univariate analysis: HR = 0.57, 95% CI: 0.46-0.70, P < 0.001; multivariate analysis: HR = 0.57, 95% CI: 0.43-0.75, P < 0.001). With univariate analysis, it was also demonstrated that miR-34a overexpression might be positively associated with a favorable OS in solid tumor (HR = 0.73, 95% CI: 0.54-1.00, P = 0.005) with considering an obvious heterogeneity. Conclusion: Our current study supports the notion that miR-34a may be a potential biomarker to predict OS and RFS/PFS/DFS/EFS in solid tumor.
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- 2015
21. MicroRNA-141 is a biomarker for progression of squamous cell carcinoma and adenocarcinoma of the lung: clinical analysis of 125 patients
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Rong-Quan He, Xinxi Hou, You Xie, Gang Chen, Minhua Rong, Ping Li, and Xiuling Zhang
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Oncology ,Adult ,Male ,medicine.medical_specialty ,Lung Neoplasms ,Kaplan-Meier Estimate ,Adenocarcinoma ,General Biochemistry, Genetics and Molecular Biology ,Young Adult ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Adenocarcinoma of the lung ,medicine ,Carcinoma ,Biomarkers, Tumor ,Humans ,Lung cancer ,Protein Kinase Inhibitors ,Aged ,Aged, 80 and over ,Univariate analysis ,business.industry ,Large cell ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Survival Analysis ,respiratory tract diseases ,ErbB Receptors ,MicroRNAs ,Mutation ,Carcinoma, Squamous Cell ,Disease Progression ,Biomarker (medicine) ,Female ,business - Abstract
Lung cancer is the most common malignant tumor worldwide. MicroRNA has become an ideal biomarker for cancer diagnosis, prognosis and therapy. The relationship between microRNA-141 and non-small cell lung cancer (NSCLC) is contradictory. Thus, in current study, we aimed to investigate the level of microRNA-141 in NSCLC tissues and to evaluate its potential clinical value. This study enrolled 125 NSCLC patients (75 males and 50 females) with a median age of 61 years (range, 23-90 years). NSCLC patients included 23 squamous cell carcinomas (SCCs), 101 adenocarcinomas (ADCs) and 1 large cell carcinoma. The expression level of microRNA-141 was significantly higher in NSCLC tissues than in adjacent lung tissues (P < 0.001), detected by real time RT-PCR. Receiver operating characteristic (ROC) exhibited a moderate diagnostic value of microRNA-141 for NSCLC with the area under curve of 0.707. The microRNA-141 expression increased with the larger tumor size (P = 0.002), lymph node metastasis (P = 0.018) and advanced stage (P = 0.022) in NSCLC patients. For subgroup analysis, microRNA-141 expression in SCC was correlated with tumor size (r = 0.490, P = 0.018), and in ADC, microRNA-141 level was positively associated with tumor size (r = 0.222, P = 0.026), lymph node metastasis (r = 0.242, P = 0.015) and TNM stage (r = 0.210, P = 0.035). Furthermore, univariate analysis revealed that the expression of microRNA-141 was an independent prognostic indicator of ADC. In conclusion, microRNA-141 is a potential biomarker for the molecular diagnosis and risk stratification of NSCLC.
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- 2015
22. Decreased expression and clinical significance of miR-148a in hepatocellular carcinoma tissues
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Gang Chen, Lin-jiang Pan, Rong-Quan He, Yi-Wu Dang, Su-Ning Huang, and Minhua Rong
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Hepatocellular carcinoma ,Biology ,Metastasis ,microRNA ,medicine ,Carcinoma ,Humans ,Clinical significance ,RNA, Messenger ,Mir 148a ,neoplasms ,Aged ,Cell Proliferation ,Aged, 80 and over ,Regulation of gene expression ,Cell growth ,Research ,Liver Neoplasms ,RT-qPCR ,Paraffin-embedded tissues ,General Medicine ,Middle Aged ,medicine.disease ,digestive system diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,miR-148a ,Female - Abstract
Background Aberrant expression of microRNA-148a (miR-148a) has been reported in several types of malignancies. However, its expression and clinicopathological significance in hepatocellular carcinoma (HCC) has not been entirely clarified. Our objective was to investigate the clinicopathological contribution of the miR-148a expression in HCC formalin-fixed paraffin-embedded (FFPE) tissues. Methods Eighty-nine HCC and their para-cancerous liver tissues were recruited. Total mRNA including miRNA was isolated and miR-148a expression was determined by using real time RT-qPCR. Furthermore, the relationship between the miR-148a level and clinicopathological features was explored. Results Significantly lower miR-148a expression in HCC tissues was observed than that in adjacent noncancerous hepatic tissues. miR-148a expression was also correlated to clinical TNM stage, metastasis, status of capsular infiltration and numbers of tumor nodes. Conclusions Underexpression of miR-148a might be associated with HCC tumorigenesis and deterioration of HCC. miR-148a might act as a suppressor miRNA of HCC and it therefore has a potential role in prognosis of HCC patients.
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- 2014
23. Effects of miR-152 on cell growth inhibition, motility suppression and apoptosis induction in hepatocellular carcinoma cells
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Minhua Rong, Gang Chen, Yi-Wu Dang, Rong-Quan He, Jing Zeng, and Dian-Zhong Luo
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Adult ,Male ,Cancer Research ,Carcinoma, Hepatocellular ,Epidemiology ,Motility ,Apoptosis ,Biology ,Real-Time Polymerase Chain Reaction ,Immunoenzyme Techniques ,Cell Movement ,microRNA ,Biomarkers, Tumor ,Tumor Cells, Cultured ,Humans ,RNA, Messenger ,Protein kinase B ,Aged ,Cell Proliferation ,Neoplasm Staging ,Retrospective Studies ,Aged, 80 and over ,Cell growth ,Reverse Transcriptase Polymerase Chain Reaction ,Liver Neoplasms ,Receptors, Tumor Necrosis Factor, Member 6b ,Public Health, Environmental and Occupational Health ,Middle Aged ,Prognosis ,Cell biology ,Blot ,Survival Rate ,MicroRNAs ,Oncology ,Liver ,Cell culture ,DNMT1 ,Cancer research ,Female ,Follow-Up Studies - Abstract
Background: miR-152 is involved in the genesis and development of several malignancies. However, its role in HCC has not been fully clarified. The aim of this study was to investigate the clinicopathological significance of miR-152 and its effect on the malignant phenotype of HCC cells. Methods: miR-152 expression was detected using real-time quantitative RT-PCR in 89 pairs of HCC formalin-fixed paraffin-embedded and their adjacent tissues. Functionally, in vitro effects and mechanisms of action of miR-152 on proliferation, viability, caspase activity, apoptosis and motility were explored in HepG2, HepB3 and SNU449 cells, as assessed by spectrophotometry, fluorimetry, fluorescence microscopy, wound-healing and Western blotting, respectively. Results: miR-152 expression in HCC was downregulated remarkably compared to that in adjacent hepatic tissues. miR-152 levels in groups of advanced clinical stage, larger tumor size and positive HBV infection, were significantly lower than in other groups. A miR-152 mimic could suppress cell growth, inhibit cell motility and increase caspase activity and apoptosis in HCC cell lines. Furthermore, Western blotting showed that the miR-152 mimic downregulated Wnt-1, DNMT1, ERK1/2, AKT and TNFRS6B signaling. Intriguingly, inverse correlation of TNFRF6B and miR-152 expression was found in HCC and bioinformatics confirmed that TNFRF6B might be a target of miR- 152. Conclusions: Underexpression of miR-152 plays a vital role in hepatocarcinogenesis and lack of miR-152 is related to the progression of HCC through deregulation of cell proliferation, motility and apoptosis. miR-152 may act as a tumor suppressor miRNA by also targeting TNFRSF6B and is therefore a potential candidate biomarker for HCC diagnosis, prognosis and molecular therapy.
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- 2014
24. Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells
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Su-Ning Huang, Gang Chen, Rong-Quan He, Yi-Wu Dang, and Minhua Rong
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Pathology ,medicine.medical_specialty ,Article Subject ,General Immunology and Microbiology ,medicine.diagnostic_test ,Cetuximab ,Cell growth ,lcsh:R ,lcsh:Medicine ,General Medicine ,Biology ,medicine.disease ,General Biochemistry, Genetics and Molecular Biology ,digestive system diseases ,Western blot ,Apoptosis ,Cell culture ,Hepatocellular carcinoma ,microRNA ,Monoclonal ,Cancer research ,medicine ,Research Article ,medicine.drug - Abstract
Previously, we found that the expression of microRNA-146a (miR-146a) was downregulated in hepatocellular carcinoma (HCC) formalin-fixed paraffin-embedded (FFPE) tissues compared to the adjacent noncancerous hepatic tissues. In the current study, we have explored thein vitroeffect of miR-146a on the malignant phenotypes of HCC cells. MiR-146a mimic could suppress cell growth and increase cellular apoptosis in HCC cell lines HepG2, HepB3, and SNU449, as assessed by spectrophotometry, fluorimetry, and fluorescence microscopy, respectively. Furthermore, western blot showed that miR-146a mimic downregulated EGFR, ERK1/2, and stat5 signalings. These effects were less potent compared to that of a siRNA targeting EGFR, a known target gene of miR-146a. Moreover, miR-146a mimic could enhance the cell growth inhibition and apoptosis induction impact of various EGFR targeting agents. The most potent combination was miR-146a mimic with cetuximab, presenting a synergistic effect. In conclusion, miR-146a plays a vital role in the cell growth and apoptosis of HCC cells and inducing miR-146a level might be a critical targeted molecular therapy strategy for HCC.
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- 2014
25. Expression and clinicopathological significance of miR-146a in hepatocellular carcinoma tissues
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Gang Chen, Minhua Rong, Yi-Wu Dang, and Rong-Quan He
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Male ,Pathology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Hepatocellular carcinoma ,oncogenes ,medicine.disease_cause ,Real-Time Polymerase Chain Reaction ,Metastasis ,law.invention ,law ,microRNA ,Carcinoma ,Medicine ,Humans ,metastasis ,Stage (cooking) ,neoplasms ,DNA Primers ,tumor biology ,Base Sequence ,business.industry ,Liver Neoplasms ,RT-qPCR ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,digestive system diseases ,miR-146a ,MicroRNAs ,Real-time polymerase chain reaction ,Suppressor ,Female ,Original Article ,pathology ,business ,Carcinogenesis ,Biomarkers ,paraffin-embedded tissues - Abstract
Background. Aberrant expression of microRNA-146a (miR-146a) has been found in several classes of cancers. However, its expression and clinicopathological contribution in hepatocellular carcinoma (HCC) has not been fully elucidated. Objective. To explore the clinicopathological significance of the miR-146a level in HCC formalin-fixed paraffin-embedded (FFPE) tissue. Methods. Eighty-five HCC samples and their para-cancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-146a expression was determined using real-time RT-PCR. Furthermore, the correlation between the miR-146a expression and clinicopathological parameters was investigated. Results. MicroRNA-146a expression in HCC tissues was lower compared with that in adjacent non-cancerous hepatic tissues. MicroRNA-146a expression was also related to clinical TNM stage, metastasis, portal vein tumor embolus, and number of tumor nodes. Conclusions. Down-regulation of miR-146a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-146a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.
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- 2013
26. Lack of Significant Association between Plasma/Serum miR-221 Expression and Poor Survival of Carcinoma: A Meta-Analysis
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Minhua Rong, Gang Chen, and Yi-Wu Dang
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Oncology ,medicine.medical_specialty ,Article Subject ,lcsh:Medicine ,Cochrane Library ,Bioinformatics ,lcsh:Technology ,General Biochemistry, Genetics and Molecular Biology ,Internal medicine ,microRNA ,Odds Ratio ,Carcinoma ,medicine ,Humans ,lcsh:Science ,Proportional Hazards Models ,General Environmental Science ,Proportional hazards model ,business.industry ,lcsh:T ,Hazard ratio ,lcsh:R ,Cancer ,General Medicine ,Odds ratio ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Meta-analysis ,lcsh:Q ,business ,Research Article - Abstract
Background. MicroRNAs (miRNAs) exhibit altered expression levels in cancers, and they may play a potential role as diagnostic and prognostic biomarkers of cancers. The aim of this meta-analysis was to summarize recent advances in miR-221 involvement in a variety of carcinomas and derive a more precise estimation of the relationship between circulating miR-221 level and survival of cancer patients.Methods. We searched online PubMed, EMBASE, and Cochrane Library up to August 2013 to identify relevant studies. Data were collected from studies comparing survival in patients with various carcinomas with higher miR-221 expression to those with lower levels. Pooled hazard ratios (HRs) of miR-221 for survival were calculated.Results. There were 4 studies included in the meta-analysis. The results of meta-analysis suggested that no significant difference in poor overall survival between miR-221 high and low groups (OR = 0.94, 95%, CI = 0.47–1.87,Z=0.17, andP=0.863).Conclusions. The current meta-analysis showed the equivalence of high and low plasma/serum miR-221 expression for carcinomas in terms of survival.
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- 2013
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27. Detection and identification of NAP-2 as a biomarker in hepatitis B-related hepatocellular carcinoma by proteomic approach
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Yuanjiao Huang, Zhiyong Zhang, Jian Qin, Rihong Zhai, Zhihua Jiang, Xiao Wei, Minhua Rong, Qi Wang, and Min He
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High rate ,business.industry ,lcsh:Cytology ,Research ,Hepatitis B ,Bioinformatics ,medicine.disease ,Proteomics ,Biochemistry ,digestive system diseases ,Nap ,Hepatocellular carcinoma ,Cancer research ,Medicine ,Biomarker (medicine) ,lcsh:QH573-671 ,business ,neoplasms ,Molecular Biology - Abstract
Background A lack of sensitive and specific biomarkers is a major reason for the high rate of Primary hepatocellular carcinoma (HCC)-related mortality. The aim of this study was to investigate potential proteomic biomarkers specific for HCC. Methods 81 patients with hepatitis B-related HCC and 33 healthy controls were randomly divided into a training set (33 HCC, 33 controls) and a testing set (48 HCC, 33 controls). Serum proteomic profiles were measured using Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy (SELDI-TOF-MS).) A classification tree was established by Biomarker Pattern Software (BPS). Candidate SELDI peaks were isolated by tricine-SDS-PAGE, identified by HPLC-MS/MS and validated by immunohistochemistry (IHC) in liver tissues. Results A total of 6 proteomic peaks (3157.33 m/z, 4177.02 m/z, 4284.79 m/z, 4300.80 m/z, 7789.87 m/z, and 7984.14 m/z) were chosen by BPS to establish a classification tree with the highest discriminatory power in the training set. The sensitivity and specificity of this classification tree were 95.92%, and 100% respectively in the testing set. A candidate marker of about 7984 m/z was isolated and identified as neutrophil-activating peptide 2 (NAP-2). IHC staining showed that NAP-2 signals were positive in HCC tissues but negative in adjacent tissues. Conclusion The NAP-2 may be a specific proteomic biomarker of hepatitis B-related HCC.
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- 2008
28. Downregulation of microRNA-132 indicates progression in hepatocellular carcinoma.
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XIN ZHANG, WEI TANG, RUISHAN LI, RONGQUAN HE, TINGQING GAN, YIHUAN LUO, GANG CHEN, and MINHUA RONG
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LIVER cancer ,MICRORNA ,CANCER invasiveness ,DOWNREGULATION ,GENE expression ,TUMOR suppressor genes - Abstract
Although miR-132 has been studied in various human tumors, few studies have investigated the role of miR-132 in hepatocellular carcinoma (HCC). The present study aimed to evaluate the associations between miR-132 and clinicopathological parameters, including recurrence, in patients with HCC. Reverse transcription-quantitative polymerase chain reaction analysis was used to detect the expression levels of miR-132 in 95 cases of HCC and their corresponding non-cancerous liver tissues. Th e associations between miR-132 expression levels and clinicopathological characteristics, including recurrence, were investigated in patients with HCC. miR-132 expression levels were significantly reduced in HCC tissues, as compared with adjacent non-cancerous tissues (1.9245±0.7564 vs. 2.7326±1.1475; P<0.001). The area under curve (AUC) of receiver operating characteristic (ROC) used to distinguish cancerous and non-cancerous tissues was 0.711 for miR-132 expression (95% confidence interval, 0.637-0.785; P<0.001) and the optimal cut-off value was 2.25. Expression levels of miR-132 were significantly reduced in the distant metastasis (P=0.031), advanced clinical TNM stage (P=0.022), hepatitis B virus-positive (P<0.001), NM23-expressed (P=0.034), high Ki-67 labeling index (LI; P=0.005) and tumor infiltration or no capsule groups (P=0.026). Spearman correlation analysis demonstrated that miR-132 was significantly correlated with hepatitis B virus infection (r=-0.351; P<0.001), NM23 (r=-0.220; P=0.032), Ki-67 LI (r=-0.264; P=0.010) and tumor capsule (r=-0.207; P=0.044). Kaplan-Meier analysis with the log-rank test indicated an approximate difference of 8 months, although miR-132 may exhibit inferior values for the prediction of recurrence in HCC patients (50.95 vs. 58.68 months; P=0.512). Therefore, the findings of the present study indicated that miR-132 is downregulated in HCC and may serve as a tumor suppressor in its progression. [ABSTRACT FROM AUTHOR]
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- 2016
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29. Underexpression of miR-34a in Hepatocellular Carcinoma and Its Contribution towards Enhancement of Proliferating Inhibitory Effects of Agents Targeting c-MET
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Dian-Zhong Luo, Minhua Rong, Gang Chen, and Yi-Wu Dang
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Male ,Pathology ,medicine.medical_treatment ,lcsh:Medicine ,Signal transduction ,ERK signaling cascade ,medicine.disease_cause ,Biochemistry ,Polymerase Chain Reaction ,Metastasis ,Targeted therapy ,chemistry.chemical_compound ,RNA interference ,Molecular cell biology ,Gastrointestinal Cancers ,Basic Cancer Research ,lcsh:Science ,Aged, 80 and over ,Multidisciplinary ,Chemistry ,Liver Neoplasms ,Signaling cascades ,Middle Aged ,Proto-Oncogene Proteins c-met ,Nucleic acids ,Phenotype ,Oncology ,Hepatocellular carcinoma ,Medicine ,Epigenetics ,Female ,Research Article ,Adult ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,C-Met ,Signaling in cellular processes ,Gastroenterology and Hepatology ,Gastrointestinal Tumors ,microRNA ,Genetics ,Cancer Detection and Diagnosis ,medicine ,Humans ,Biology ,Aged ,DNA Primers ,STAT signaling family ,Cell growth ,lcsh:R ,Cancers and Neoplasms ,Hepatocellular Carcinoma ,HCCS ,medicine.disease ,MicroRNAs ,Cancer research ,RNA ,lcsh:Q ,Gene expression ,Carcinogenesis - Abstract
Aberrant expression of microRNA-34a (miR-34a) has been reported to be involved in the tumorigenesis and progression of various classes of malignancies. However, its role in hepatocellular carcinoma (HCC) has not been completely clarified. In the current study, we have investigated the clinical significance and the in vitro contribution of miR-34a on biological functions of human HCCs. miR-34a expression in eighty-three cases of HCC formalin-fixed paraffin-embedded (FFPE) tissues decreased significantly compared to that in the adjacent liver tissues (P
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- 2013
30. Increased MiR-221 expression in hepatocellular carcinoma tissues and its role in enhancing cell growth and inhibiting apoptosis in vitro
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Minhua Rong, Yi-Wu Dang, and Gang Chen
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Cancer Research ,biology ,Cell growth ,MiR-221 ,Caspase 3 ,Apoptosis ,Cell cycle ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Molecular biology ,lcsh:RC254-282 ,Caspase ,digestive system diseases ,Oncology ,Cell culture ,microRNA ,Cancer research ,biology.protein ,Genetics ,Stem cell ,Hepatocellular carcinoma (HCC) ,Research Article - Abstract
Background MiR-221 is over-expressed in human hepatocellular carcinoma (HCC), but its clinical significance and function in HCC remains uncertain. The aim of the study was to investigate the relationship between miR-221 overexpression and clinicopathological parameters in HCC formalin-fixed paraffin-embedded (FFPE) tissues, and the effect of miR-221 inhibitor and mimic on different HCC cell lines in vitro. Methods MiR-221 expression was detected using real time RT-qPCR in FFPE HCC and the adjacent noncancerous liver tissues. The relationship between miR-221 level and clinicopathological features was also analyzed. Furthermore, miR-221 inhibitor and mimic were transfected into HCC cell lines HepB3, HepG2 and SNU449. The effects of miR-221 on cell growth, cell cycle, caspase activity and apoptosis were also investigated by spectrophotometry, fluorimetry, fluorescence microscopy and flow cytometry, respectively. Results The relative expression of miR-221 in clinical TNM stages III and IV was significantly higher than that in the stages I and II. The miR-221 level was also upregulated in the metastatic group compared to the nonmetastatic group. Furthermore, miR-221 over-expression was related to the status of tumor capsular infiltration in HCC clinical samples. Functionally, cell growth was inhibited, cell cycle was arrested in G1/S-phase and apoptosis was increased by miR-221 inhibitor in vitro. Likewise, miR-221 mimic accelerated the cell growth. Conclusions Expression of miR-221 in FFPE tissues could provide predictive significance for prognosis of HCC patients. Moreover, miR-221 inhibitor could be useful to suppress proliferation and induce apoptosis in HCC cells. Thus miR-221 might be a critical targeted therapy strategy for HCC.
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- 2013
31. Association between underexpression of microrna-203 and clinicopathological significance in hepatocellular carcinoma tissues.
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Yongru Liu, Fanghui Ren, Minhua Rong, Yihuan Luo, Yiwu Dang, and Gang Chen
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MICRORNA ,LIVER cancer ,BIOMARKERS ,PARAFFIN wax ,FORMALDEHYDE ,REVERSE transcriptase polymerase chain reaction ,METASTASIS ,GENETICS - Abstract
Background: Although recent studies have shown the utility of miR-203 as a cancer-relevant biomarker, the validated clinical significance of miR-203 in HCC remains obscure. The aim of the present study was to evaluate the relationship between miR-203 expression and clinicopathological features in HCC patients. Methods: MiR-203 expression in 95 formalin-fixed, paraffin embedded (FFPE) HCC tissues and their paired adjacent non-cancerous tissues was evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Simultaneously, expression of miR-203 and its correlation with a variety of clinicopathological parameters and patient recurrence was analyzed. Results: The relative level of miR-203 was 1.1651 ± 0.70378 in HCC tissues, significantly lower than its expression in the corresponding adjacent non-cancerous liver tissues (2.2408 ± 0.75351, P < 0.001). The area under curve (AUC) of low miR-203 expression to diagnose HCC was 0.85 (95 % CI: 0.796 ~ 0.904, P = 0.027) at a cut-off value 1.99 evaluated by the median expression of miR-203 in all tissues, including HCC and normal liver tissues. Expression of miR-203 was negatively correlated to metastasis (r = -0.254, P = 0.013), clinical tumor nodes metastasis (TNM) stage (r = -0.300, P = 0.003), nm23 expression (r = -0.292, P = 0.004), p21 expression (r = -0.223, P = 0.030), microvessel density (MVD)(r = -0.206, P = 0.045) and was positively correlated to cirrhosis (r = 0.487, P < 0.001). Additionally, the recurrent time of lower miR-203 expression group was 57.949 ± 4.184 months, slightly longer than that in the high expression group (54.682 ± 2.591 months), however, no significant difference was noted (Chi-square = 0.206, P = 0.650). Conclusions: MiR-203 plays a vital role in the carcinogenesis and progression of HCC, which makes itself as a predictor for the deterioration of HCC. Furthermore, miR-203 may become a new target for molecular therapy in HCC. [ABSTRACT FROM AUTHOR]
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- 2015
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32. MicroRNA-141 Is a Biomarker for Progression of Squamous Cell Carcinoma and Adenocarcinoma of the Lung: Clinical Analysis of 125 Patients.
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Xiuling Zhang, Ping Li, Minhua Rong, Rongquan He, Xinxi Hou, You Xie, and Gang Chen
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Lung cancer is the most common malignant tumor worldwide. MicroRNA has become an ideal biomarker for cancer diagnosis, prognosis and therapy. The relationship between microRNA-141 and non-small cell lung cancer (NSCLC) is contradictory. Thus, in current study, we aimed to investigate the level of microRNA-141 in NSCLC tissues and to evaluate its potential clinical value. This study enrolled 125 NSCLC patients (75 males and 50 females) with a median age of 61 years (range, 23-90 years). NSCLC patients included 23 squamous cell carcinomas (SCCs), 101 adenocarcinomas (ADCs) and 1 large cell carcinoma. The expression level of microRNA-141 was significantly higher in NSCLC tissues than in adjacent lung tissues (P < 0.001), detected by real time RT-PCR. Receiver operating characteristic (ROC) exhibited a moderate diagnostic value of microRNA-141 for NSCLC with the area under curve of 0.707. The microRNA-141 expression increased with the larger tumor size (P = 0.002), lymph node metastasis (P = 0.018) and advanced stage (P = 0.022) in NSCLC patients. For subgroup analysis, microRNA-141 expression in SCC was correlated with tumor size (r - 0.490, P - 0.018), and in ADC, microRNA-141 level was positively associated with tumor size (r = 0.222, P - 0.026), lymph node metastasis (r = 0.242, P - 0.015) and TNM stage (r - 0.210, P - 0.035). Furthermore, univariate analysis revealed that the expression of microRNA-141 was an independent prognostic indicator of ADC. In conclusion, microRNA-141 is a potential biomarker for the molecular diagnosis and risk stratification of NSCLC. [ABSTRACT FROM AUTHOR]
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- 2015
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33. Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells.
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Suning Huang, Rongquan He, Minhua Rong, Yiwu Dang, and Gang Chen
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Previously, we found that the expression of microRNA-146a (miR-146a) was downregulated in hepatocellular carcinoma (HCC) formalin-fixed paraffin-embedded (FFPE) tissues compared to the adjacent noncancerous hepatic tissues. In the current study, we have explored the in vitro effect of miR-146a on the malignant phenotypes ofHCC cells. MiR-146a mimic could suppress cell growth and increase cellular apoptosis in HCC cell lines HepG2, HepB3, and SNU449, as assessed by spectrophotometry, fluorimetry, and fluorescence microscopy, respectively. Furthermore, western blot showed that miR-146a mimic downregulated EGFR, ERK1/2, and stat5 signalings. These effects were less potent compared to that of a siRNA targeting EGFR, a known target gene of miR-146a. Moreover, miR-146a mimic could enhance the cell growth inhibition and apoptosis induction impact of various EGFR targeting agents. The most potent combination was miR-146a mimic with cetuximab, presenting a synergistic effect. In conclusion, miR-146a plays a vital role in the cell growth and apoptosis of HCC cells and inducing miR-146a level might be a critical targeted molecular therapy strategy for HCC. [ABSTRACT FROM AUTHOR]
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- 2014
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34. Detection and identification of NAP-2 as a biomarker in hepatitis B-related hepatocellular carcinoma by proteomic approach.
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Min He, Jian Qin, Rihong Zhai, Xiao Wei, Qi Wang, Minhua Rong, Zhihua Jiang, Yuanjiao Huang, and Zhiyong Zhang
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BIOMARKERS ,BLOOD proteins ,LIVER cancer ,HEPATITIS B ,PROTEOMICS ,DIAGNOSTIC immunohistochemistry - Abstract
Background: A lack of sensitive and specific biomarkers is a major reason for the high rate of Primary hepatocellular carcinoma (HCC)-related mortality. The aim of this study was to investigate potential proteomic biomarkers specific for HCC. Methods: 81 patients with hepatitis B-related HCC and 33 healthy controls were randomly divided into a training set (33 HCC, 33 controls) and a testing set (48 HCC, 33 controls). Serum proteomic profiles were measured using Surface-enhanced laser desorption/ionization-time-off-light mass spectroscopy (SELDI-TOF-MS).) A classification tree was established by Biomarker Pattern Software (BPS). Candidate SELDI peaks were isolated by tricine-SDS-PAGE, identified by HPLC-MS/MS and validated by immunohistochemistry (IHC) in liver tissues. Results: A total of 6 proteomic peaks (3157.33 m/z, 4177.02 m/z, 4284.79 m/z, 4300.80 m/z, 7789.87 m/z, and 7984.14 m/z) were chosen by BPS to establish a classification tree with the highest discriminatory power in the training set. The sensitivity and specificity of this classification tree were 95.92%, and 100% respectively in the testing set. A candidate marker of about 7984 m/z was isolated and identified as neutrophil-activating peptide 2 (NAP-2). IHC staining showed that NAP-2 signals were positive in HCC tissues but negative in adjacent tissues. Conclusion: The NAP-2 may be a specific proteomic biomarker of hepatitis B-related HCC. [ABSTRACT FROM AUTHOR]
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- 2008
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35. Association between underexpression of microrna-203 and clinicopathological significance in hepatocellular carcinoma tissues
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Yongru Liu, Gang Chen, Minhua Rong, Fanghui Ren, Yi-Wu Dang, and Yihuan Luo
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Pathology ,medicine.medical_specialty ,Cancer Research ,MiR-203 ,business.industry ,RT-qPCR ,medicine.disease ,digestive system diseases ,Metastasis ,Adjacent non-cancerous liver ,Oncology ,Hepatocellular carcinoma ,microRNA ,medicine ,Cancer research ,Genetics ,Clinicopathological features ,Biomarker (medicine) ,Clinical significance ,HCC ,Primary Research ,business ,miR-203 - Abstract
Background Although recent studies have shown the utility of miR-203 as a cancer-relevant biomarker, the validated clinical significance of miR-203 in HCC remains obscure. The aim of the present study was to evaluate the relationship between miR-203 expression and clinicopathological features in HCC patients. Methods MiR-203 expression in 95 formalin-fixed, paraffin embedded (FFPE) HCC tissues and their paired adjacent non-cancerous tissues was evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Simultaneously, expression of miR-203 and its correlation with a variety of clinicopathological parameters and patient recurrence was analyzed. Results The relative level of miR-203 was 1.1651 ± 0.70378 in HCC tissues, significantly lower than its expression in the corresponding adjacent non-cancerous liver tissues (2.2408 ± 0.75351, P
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