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Identification of prognostic splicing factors and exploration of their potential regulatory mechanisms in pancreatic adenocarcinoma
- Source :
- PeerJ, PeerJ, Vol 8, p e8380 (2020)
- Publication Year :
- 2019
-
Abstract
- Pancreatic adenocarcinoma (PAAD), the most common subtype of pancreatic cancer, is a highly lethal disease. In this study, we integrated the expression profiles of splicing factors (SFs) of PAAD from RNA-sequencing data to provide a comprehensive view of the clinical significance of SFs. A prognostic index (PI) based on SFs was developed using the least absolute shrinkage and selection operator (LASSO) COX analysis. The PI exhibited excellent performance in predicting the status of overall survival of PAAD patients. We also used the percent spliced in (PSI) value obtained from SpliceSeq software to quantify different types of alternative splicing (AS). The prognostic value of AS events was explored using univariate COX and LASSO COX analyses; AS-based PIs were also proposed. The integration of prognosis-associated SFs and AS events suggested the potential regulatory mechanisms of splicing processes in PAAD. This study defined the markedly clinical significance of SFs and provided novel insight into their potential regulatory mechanisms.
- Subjects :
- musculoskeletal diseases
Bioinformatics
RNA-sequencing
lcsh:Medicine
Disease
Computational biology
Biology
General Biochemistry, Genetics and Molecular Biology
Splicing factors
03 medical and health sciences
0302 clinical medicine
Lasso (statistics)
Pancreatic cancer
Evidence Based Medicine
medicine
Overall survival
Clinical significance
030304 developmental biology
0303 health sciences
General Neuroscience
lcsh:R
Alternative splicing
General Medicine
medicine.disease
Oncology
030220 oncology & carcinogenesis
RNA splicing
Adenocarcinoma
General Agricultural and Biological Sciences
Medical Genetics
Pancreatic adenocarcinoma
Subjects
Details
- ISSN :
- 21678359
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....a025e587c320829e0ebdeecfeb915016