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2. Distinguishing the four genetic causes of Jouberts syndrome-related disorders

Author

Valente, E M, Marsh, S E, Castori, M, Dixon-Salazar, T, Bertini, E, Al-Gazali, L, Messer, J, Barbot, C, Woods, C G, Boltshauser, E, Al-Tawari, A A, Salpietro, C D, Kayserili, H, Sztriha, L, Gribaa, M, Koenig, M, Dallapiccola, B, and Gleeson, J G

Abstract

Jouberts syndrome-related disorders are a group of recessively inherited conditions showing cerebellar vermis hypoplasia and the molar tooth sign of the midbrain-hindbrain junction. Recent analyses have suggested at least three loci, JBTS1 (9q34-3), -2 (11p11.2-q12.3), and -3 (6q23), but the phenotypic spectrum associated with each locus has not been delineated. In addition, deletions of the NPHP1 gene, usually responsible for isolated juvenile nephronophthisis, are occasionally encountered among Jouberts syndrome-related disorder patients. Here, we describe four novel families showing evidence of linkage to two of these loci, provide a 3.6Mb refinement of the JBTS2 locus, and perform a detailed comparison of all linked families identified so far, to define the clinical and radiographical hallmarks for each genetic condition. We find that JBTS1 and -3 primarily show features restricted to the central nervous system, with JBTS1 showing largely pure cerebellar and midbrain-hindbrain junction involvement, and JBTS3 displaying cerebellar, midbrain-hindbrain junction, and cerebral cortical features, most notably polymicrogyria. Conversely, JBTS2 is associated with multiorgan involvement of kidney, retina, and liver, in addition to the central nervous system features, and results in extreme phenotypic variability. This provides a useful framework for genetic testing strategies and prediction of which patients are most likely to experience development of systemic complications.

Published
2005

3. Effect of Intra- and Extrauterine Growth on Long-Term Neurologic Outcomes of Very Preterm Infants

Author

Larroque, B., Ancel, P.Y., Blondel, B., Bréart, G., Dehan, M., Garel, M., Kaminski, M., Maillard, F., du Mazaubrun, C., Missy, P., Sehili, F., Supernant, K., Marchand, L., Durand, M., Matis, J., Messer, J., Treisser, A., Burguet, A., Abraham-Lerat, L., Menget, A., Roth, P., Schaal, J.-P., Thiriez, G., Leveque, C., Marret, S., Marpeau, L., Boulot, P., Cambonie, G., Picaud, J.-C., Donadio, A.M., Ledesert, B., André, M., Fresson, J., Hascoët, J.M., Arnaud, C., Grandjean, H., Rolland, M., Fily, A., Ego, A., Outtier, M.L., Pierrat, V., Subtil, D., Truffert, P., Boog, G., Rouger-Bureau, V., Rozé, J.-C., Zupan-Simunek, V., Vodovar, M., Voyer, M., Guellec, Isabelle, Lapillonne, Alexandre, Marret, Stephane, Picaud, Jean-Charles, Mitanchez, Delphine, Charkaluk, Marie-Laure, Fresson, Jeanne, Arnaud, Catherine, Flamand, Cyril, Cambonie, Gilles, Kaminski, Monique, Roze, Jean-Christophe, and Ancel, Pierre-Yves

Published
2016
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4. Persistence of Literacy Problems: Spelling in Adolescence and at Mid-Life

Author

Maughan, Barbara, Messer, J., Collishaw, S., Pickles, A., Snowling, M., Yule, W., and Rutter, M.

Abstract

Background: Developmental reading problems show strong persistence across the school years; less is known about poor readers' later progress in literacy skills. Method: Poor (n = 42) and normally developing readers (n = 86) tested in adolescence (ages 14/15 years) in the Isle of Wight epidemiological studies were re-contacted at mid-life (ages 44/45 years). Participants completed a spelling test, and reported on educational qualifications, perceived adult spelling competence, and problems in day-to-day literacy tasks. Results: Individual differences in spelling were highly persistent across this 30-year follow-up, with correlations between spelling at ages 14 and 44 years of r = 0.91 (p less than 0.001) for poor readers and r = 0.89 (p less than 0.001) for normally developing readers. Poor readers' spelling remained markedly impaired at mid-life, with some evidence that they had fallen further behind over the follow-up period. Taking account of adolescent spelling levels, continued exposure to reading and literacy demands in adolescence and early adulthood was independently predictive of adult spelling in both samples; family social background added further to prediction among normally developing readers only. Conclusions: By adolescence, individual differences in spelling and its related sub-skills are highly stable. Encouraging young people with reading disabilities to maintain their exposure to reading and writing may be advantageous in the longer term.

Published
2009
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5. Amplifying patient voices amid pandemic: Perspectives on tracheostomy care, communication, and connection.

Author

Pandian, V, Hopkins, BS, Yang, CJ, Ward, E, Sperry, ED, Khalil, O, Gregson, P, Bonakdar, L, Messer, J, Messer, S, Chessels, G, Bosworth, B, Randall, DM, Freeman-Sanderson, A, McGrath, BA, Brenner, MJ, Pandian, V, Hopkins, BS, Yang, CJ, Ward, E, Sperry, ED, Khalil, O, Gregson, P, Bonakdar, L, Messer, J, Messer, S, Chessels, G, Bosworth, B, Randall, DM, Freeman-Sanderson, A, McGrath, BA, and Brenner, MJ

Abstract

OBJECTIVE: To investigate perspectives of patients, family members, caregivers (PFC), and healthcare professionals (HCP) on tracheostomy care during the COVID-19 pandemic. METHODS: The cross-sectional survey investigating barriers and facilitators to tracheostomy care was collaboratively developed by patients, family members, nurses, speech-language pathologists, respiratory care practitioners, physicians, and surgeons. The survey was distributed to the Global Tracheostomy Collaborative's learning community, and responses were analyzed. RESULTS: Survey respondents (n = 191) from 17 countries included individuals with a tracheostomy (85 [45 %]), families/caregivers (43 [22 %]), and diverse HCP (63 [33.0 %]). Overall, 94 % of respondents reported concern that patients with tracheostomy were at increased risk of critical illness from SARS-CoV-2 infection and COVID-19; 93 % reported fear or anxiety. With respect to prioritization of care, 38 % of PFC versus 16 % of HCP reported concern that patients with tracheostomies might not be valued or prioritized (p = 0.002). Respondents also differed in fear of contracting COVID-19 (69 % PFC vs. 49 % HCP group, p = 0.009); concern for hospitalization (55.5 % PFC vs. 27 % HCP, p < 0.001); access to medical personnel (34 % PFC vs. 14 % HCP, p = 0.005); and concern about canceled appointments (62 % PFC vs. 41 % HCP, p = 0.01). Respondents from both groups reported severe stress and fatigue, sleep deprivation, lack of breaks, and lack of support (70 % PFC vs. 65 % HCP, p = 0.54). Virtual telecare seldom met perceived needs. CONCLUSION: PFC with a tracheostomy perceived most risks more acutely than HCP in this global sample. Broad stakeholder engagement is necessary to achieve creative, patient-driven solutions to maintain connection, communication, and access for patients with a tracheostomy.

Published
2022

9. Ther Adv Neurol Disord

Author

Wundes, A. (Annette), Wray, S. (Sibyl), Gold, R. (Ralf), Singer, Barry A.A. (A), Jasinska, E. (Elzbieta), Ziemssen, T. (Tjalf), De Sèze, J. (Jérôme), Repovic, P. (Pavle), Chen, H. (Hailu), Hanna, J. (Jerome), Messer, J. (Jordan), Miller, C. (Catherine), and Naismith, Robert T.T. (T)

Subjects
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
Abstract

Background: Diroximel fumarate (DRF) is a novel oral fumarate approved for relapsing forms of multiple sclerosis (MS). DRF demonstrated significantly improved gastrointestinal (GI) tolerability versus dimethyl fumarate (DMF) with fewer days of Individual Gastrointestinal Symptom and Impact Scale (IGISIS) scores ⩾2, GI adverse events (AEs), and treatment discontinuations due to GI AEs. Our aim was to evaluate the impact of GI tolerability events on quality of life (QoL) for patients with relapsing-remitting MS who received DRF or DMF in EVOLVE-MS-2. Methods: A post hoc analysis was conducted in patients who were enrolled in the randomized, blinded, 5-week, EVOLVE-MS-2 [ClinicalTrials.gov identifier: NCT03093324] study of DRF versus DMF. Patients completed daily IGISIS and Global GISIS (GGISIS) eDiary questionnaires to assess GI symptom intensity and interference with daily activities and work. Results: In total, 504 patients (DRF, n = 253; DMF, n = 251) received study drug and 502 (DRF, n = 253; DMF, n = 249) completed at least one post-baseline questionnaire. With DRF, GI symptoms were less likely to interfere 'quite a bit' or 'extremely' with regular daily activities [IGISIS: DRF, 9.5% (24/253) versus DMF, 28.9% (72/249)] or work productivity [GGISIS: DRF, 6.1% (10/165) versus DMF, 11.3% (18/159)]. DRF-treated patients had fewer days with ⩾1 h of missed work (DRF, 43 days, n = 20 versus DMF, 88 days, n = 26). DMF-treated patients reported highest GI symptom severity and missed work at week 2-3 shortly after completing the titration period, which coincided with the majority of GI-related treatment discontinuations [58.3% (7/12)]. GI tolerability AEs [DRF, 34.8% (88/253); DMF, 48.2% (121/251)], concomitant symptomatic medication use [DRF, 19.3% (17/88) versus DMF, 30.6% (37/121)], and GI-related discontinuations (DRF, 0.8% versus DMF, 4.8%) were lower with DRF versus DMF. Conclusions: The improved GI tolerability with DRF translated into clinically meaningful benefits to QoL, as patients experienced less impact on daily life and work and required less concomitant symptomatic medication use. Trial registration: [ClinicalTrials.gov identifier: NCT03093324].

Published
2021

10. The Effective Mass of a Ball in the Air

Author

Messer, J. and Pantaleone, J.

Abstract

The air surrounding a projectile affects the projectile's motion in three very different ways: the drag force, the buoyant force, and the added mass. The added mass is an increase in the projectile's inertia from the motion of the air around it. Here we experimentally measure the added mass of a spherical projectile in air. The results agree well with the calculation of the added mass for an ideal fluid. By accounting for the added mass, high school or undergraduate students can easily measure the acceleration of gravity accurately with a beach ball projectile.

Published
2010
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17. The U.S. Environmental Protection Agency’s Environmental Monitoring and Assessment Program

Author

Jones, K, primary, Bolgrien, D, additional, Levinson, B, additional, Heggem, D, additional, McDonald, M, additional, Hedtke, S, additional, Hale, S, additional, Veith, G, additional, Paul, J, additional, Blair, R, additional, Summers, K, additional, Paulsen, S, additional, Messer, J, additional, Jackson, L, additional, Olsen, A, additional, Linthurst, R, additional, Dlugosz, J, additional, Brown, B, additional, and Stoddard, J, additional

Published
2004
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33. Copper chelation assay for histamine in tuna

Author

Bateman, R.C., Jr., Eldrige, D.B., Wade, S., McCoy-Messer, J., Jester, E.L.E., and Mowdy, D.E.

Subjects
Histamine -- Analysis, Cookery (Tuna) -- Analysis, Business, Food/cooking/nutrition
Abstract

A copper chelation method identifies a biogenic amine, histamine, in yellowfin tuna steaks. This method requires cation exchange chromatography to purify histamine and a fluorometer for product identification. Scombroid fish undergoes microbial decomposition to generate histamine, which causes scombroid poisoning.

Published
1994

40. Effect of Intra- and Extrauterine Growth on Long-Term Neurologic Outcomes of Very Preterm Infants

Author

Guellec, Isabelle, Lapillonne, Alexandre, Picaud, Jean-Charles, Mitanchez, Delphine, Charkaluk, Marie-Laure, Flamand, Cyril, Roze, Jean-Christophe, Ancel, Pierre-Yves, Larroque, Benoit, Ancel, P.Y., Blondel, B., Bréart, G., Garel, M., Kaminski, Monique, Maillard, F., du Mazaubrun, C., Missy, P., Sehili, F., Supernant, K., Marchand, L., Durand, M., Matis, J., Messer, J., Treisser, A., Burguet, A., Abraham-Lerat, L., Menget, A., Roth, P., Schaal, P., Thiriez, G., Leveque, C., Marret, Stéphane, Marpeau, L., Boulot, P., Cambonie, Gilles, Picaud, C., Donadio, M., Ledésert, B., André, M., Fresson, Jeanne, Hascoët, M., Arnaud, Catherine, Grandjean, H., Rolland, M., Fily, A., Ego, Anne, Outtier, M., Pierrat, V., Subtil, D., Truffert, P., Boog, G., Rouger-Bureau, V., Rozé, C., Dehan, M., Zupan-Simunek, V., Vodovar, M., Voyer, M., Equipe 1 : EPOPé - Épidémiologie Obstétricale, Périnatale et Pédiatrique (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Necker - Enfants Malades [AP-HP], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), CHU Trousseau [APHP], Centre hospitalier universitaire de Nantes (CHU Nantes), Département de Périnatologie, Hôpital Mère Enfant, Recherches épidémiologiques en santé périnatale et santé des femmes, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), CHU Rouen, Normandie Université (NU), Service de Gynécologie-Obstétrique et Médecine de la Reproduction [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Université de Cergy Pontoise (UCP), Université Paris-Seine, Swedish Institute of Space Physics [Uppsala] (IRF), Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), DGA Tech. Navales, DGA Tech.Navales, Service de Médecine Néonatale, Hôpital Jeanne de Flandre [Lille], Techniques pour l'Evaluation et la Modélisation des Actions de la Santé (TIMC-IMAG-ThEMAS), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département d'obstétrique[Lille], Hôpital Jeanne de Flandre [Lille]-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Biologie et physiologie des cellules digestives, Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de recherche clinique intersectorielle du Centre Hospitalier Henri Laborit, Centre Hospitalier Henri Laborit (CHL), Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université, Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Ferrand (UFR STAPS - UBP), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Techniques pour l'Evaluation et la Modélisation des Actions de la Santé (TIMC-ThEMAS), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-IMAG-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-IMAG-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), and Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])

Subjects
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

42. Natural atypical Listeria innocua strains with Listeria monocytogenes pathogenicity island 1 genes

Author

Johnson, J., Jinneman, K., Stelma, G., Smith, B. G., Lye, D., Messer, J., Ulaszek, J., Evsen, L., Gendel, S., Bennett, R. W., Swaminathan, B., Pruckler, J., Steigerwalt, A., Kathariou S., Yildirim, S., Volokhov, D., Rasooly, A., Chizhikov V., Weidmann, M., Fortes, E., Duvall, R. E., and Hitchins, A. D.

Subjects
Virulence (Microbiology) -- Analysis, Listeria monocytogenes -- Analysis, Microbiology -- Analysis, Biological sciences
Abstract

Generally only a few tests are necessary to classify the bona fide Listeria isolates in the six species of the genus. The Listeria innocua isolate comprises of the members of PrfA, which is the standard accumulation of virulence genes of Listeria monocytogenes.

Published
2004

46. Effects of Antenatal Antibiotics on the Incidence and Bacteriological Profile of Early-Onset Neonatal Sepsis

Author

B Escande, V. Laugel, J Beladdale, D Astruc, P. Kuhn, L. Donato, and Messer J

Subjects
Pediatrics, medicine.medical_specialty, Neonatal sepsis, medicine.drug_class, business.industry, Incidence (epidemiology), Antibiotics, Retrospective cohort study, medicine.disease, Group B, Antibiotic resistance, Pediatrics, Perinatology and Child Health, Epidemiology, medicine, business, Developmental Biology, Antibacterial agent
Abstract

Background: Recommendations for the use of antenatal antibiotics have been widely implemented in the past few years, notably to prevent group B streptococcal disease or to prolong pregnancy in the case of preterm premature rupture of the membranes. Objectives: We designed a retrospective study to assess the potential effects of this increasing use of antibiotics on the incidence and bacteriological profile of early-onset neonatal sepsis (EONS). Methods: All neonates referred to our department for suspected EONS from January 1 1995 through December 31 1999 were included. Antenatal antibiotic exposure together with clinical and microbiological data from the neonatal period were gathered and analyzed on a yearly basis. Results: Of the 485 newborns who met the inclusion criteria, there were 101 cases of culture-confirmed sepsis; 339 cases of suspected sepsis and 69 cases of confirmed sepsis involved children born in the hospital, among a total of 16,627 live births registered in our center over the study period. The overall incidence of EONS dropped from 6.8 to 0.6/1,000 births between 1995 and 1999 (p < 0.001), but the rate of group B streptococcal infection decreased much more rapidly than that of non-group B streptococcal infection. We observed a trend towards the emergence of ampicillin-resistant Escherichia coli strains, which were isolated in seven cases. Among E. coli infections, ampicillin resistance was statistically linked with antenatal antibiotic use (p = 0.025). We also delineated several risk factors associated with these infections. Conclusion: In our center, antenatal antibiotic treatment was effective in reducing the incidence of EONS, but this benefit may come at the cost of favoring the emergence of ampicillin-resistant organisms causing severe neonatal infections. Antenatal and postnatal antibiotic treatment strategies should take this adverse effect into account.

Published
2003

48. Impaired glycolysis and protein catabolism induced by acid in L6 rat muscle cells

Author

Pratt A, Messer J, John Walls, J. Brown, and Alan Bevington

Subjects
Glucose uptake, Intracellular pH, Clinical Biochemistry, Protein turnover, Skeletal muscle, General Medicine, Protein degradation, Biology, Biochemistry, Protein catabolism, medicine.anatomical_structure, Anaerobic glycolysis, medicine, Glycolysis
Abstract

Background In skeletal muscle, metabolic acidosis stimulates protein degradation and oxidation of branched-chain amino acids. This could occur to compensate for impairment of glucose utilization induced by acid. Methods To test this hypothesis, glycolysis and protein degradation (release of [14C]-phenylalanine) were measured in L6 skeletal muscle cells cultured in Eagle's minimum essential medium at pH 7.1 or 7.5 for up to 3 days. Results No marked changes in total DNA or in cell viability were detected, nor was there any significant effect on intracellular pH or the water content of the cells (which is thought to be a key regulator of protein turnover, especially in liver). In spite of this, acid stimulated protein degradation, induced net protein loss from the cultures, inhibited glucose uptake and glycolysis (lactate output) and was associated with increased [1-14C]-leucine oxidation. Effects on protein degradation and glycolysis were gradual, reaching a maximum after 20–30 h. To investigate whether glycolytic flux itself can influence protein degradation, increased glycolysis was simulated by adding glucose (20 mmol L−1) or pyruvate (1 mmol L−1) to the medium. At pH 7.1, neither addition had any effect on protein degradation. Conclusion Although acid-induced protein wasting is associated with impaired glycolysis, no obligatory coupling exists between glycolytic flux and protein degradation.

Published
1998

50. Bilan hydrique urbain de revêtements de béton poreux optimisé pour évaporation

Author

Göbel, P., Starke, P., Messer, J., Coldewey, W., University of Münster, and Brelot, Elodie

Subjects
[SDE.IE]Environmental Sciences/Environmental Engineering, Performance, Permeable pavement, Revêtement poreux
Abstract

Colloque avec actes et comité de lecture. Internationale.; International audience

Published
2013

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