330 results on '"Makiko Shimizu"'
Search Results
2. Metabolic Syndrome and the Increased Risk of Medically Certified Long-term Sickness Absence: A Prospective Analysis Among Japanese Workers
- Author
-
Dong V. Hoang, Shamima Akter, Yosuke Inoue, Keisuke Kuwahara, Ami Fukunaga, Zobida Islam, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Hiroko Okazaki, Toshiaki Miyamoto, Takayuki Ogasawara, Naoko Sasaki, Akihiko Uehara, Makoto Yamamoto, Takeshi Kochi, Masafumi Eguchi, Taiki Shirasaka, Makiko Shimizu, Satsue Nagahama, Ai Hori, Teppei Imai, Akiko Nishihara, Kentaro Tomita, Chihiro Nishiura, Maki Konishi, Isamu Kabe, Kenya Yamamoto, Tetsuya Mizoue, and Seitaro Dohi
- Subjects
sickness absence ,metabolic syndrome ,longitudinal study ,japan ,Medicine (General) ,R5-920 - Abstract
Background: Metabolic syndrome (MetS) has been associated with various chronic diseases that may lead to long-term sickness absence (LTSA), but there is lacking information on the direct association between MetS and LTSA. The present study aimed to investigate the all-cause and cause-specific associations between MetS and the risk of medically certified LTSA among Japanese workers. Methods: We recruited 67,403 workers (57,276 men and 10,127 women), aged 20–59 years from 13 companies in Japan during their health check-ups in 2011 (11 companies) and 2014 (2 companies), and we followed them for LTSA events (≥30 consecutive days) until March 31, 2020. MetS was defined according to the Joint Interim Statement. A Cox proportional hazards regression model was used to estimate hazard ratios (HRs) and its 95% confidence intervals (CIs) for LTSA associated with MetS and its components. Results: During 408,324 person-years of follow-up, 2,915 workers experienced LTSA. The adjusted HR for all-cause LTSA was 1.54 (95% CI, 1.41–1.68) among those with MetS compared to those without MetS. In cause-specific analysis, HRs associated with MetS significantly increased for LTSA due to overall physical disorders (1.76); cardiovascular diseases (3.16); diseases of the musculoskeletal system and connective tissue (2.01); cancers (1.24); obesity-related cancers (1.35); mental, behavioral, and neurodevelopmental disorders (1.28); reaction to severe stress and adjustment disorders (1.46); and external causes (1.46). The number of MetS components were also significantly associated with increased LTSA risk. Conclusion: MetS was associated with an increase in the risk of LTSA due to various diseases among Japanese workers.
- Published
- 2023
- Full Text
- View/download PDF
3. Deschloroclozapine exhibits an exquisite agonistic effect at lower concentration compared to clozapine-N-oxide in hM3Dq expressing chemogenetically modified rats
- Author
-
Makiko Shimizu, Mitsuhiro Yoshimura, Kazuhiko Baba, Naofumi Ikeda, Yuki Nonaka, Takashi Maruyama, Tatsushi Onaka, and Yoichi Ueta
- Subjects
deschloroclozapine ,clozapine-N-oxide ,DREADDs ,oxytocin ,central nervous system ,Fos ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
IntroductionWithin the realm of chemogenetics, a particular form of agonists targeting designer receptors exclusively activated by designer drugs (DREADDs) has emerged. Deschloroclozapine (DCZ), a recently introduced DREADDs agonist, demonstrates remarkable potency in activating targeted neurons at a lower dosage compared to clozapine-N-oxide (CNO).MethodsWe conducted a comparative analysis of the effects of subcutaneously administered CNO (1 mg/kg) and DCZ (0.1 mg/kg) in our transgenic rats expressing hM3Dq and mCherry exclusively in oxytocin (OXT) neurons.Results and DiscussionNotably, DCZ exhibited a swift and robust elevation of serum OXT, surpassing the effects of CNO, with a significant increase in the area under the curve (AUC) up to 3 hours post-administration. Comprehensive assessment of brain neuronal activity, using Fos as an indicator, revealed comparable effects between CNO and DCZ. Additionally, in a neuropathic pain model, both CNO and DCZ increased the mechanical nociceptive and thermal thresholds; however, the DCZ-treated group exhibited a significantly accelerated onset of the effects, aligning harmoniously with the observed alterations in serum OXT concentration following DCZ administration. These findings emphasize the remarkable efficacy of DCZ in rats, suggesting its equivalent or potentially superior performance to CNO at considerably lower dosages, thus positioning it as a promising contender among DREADDs agonists.
- Published
- 2023
- Full Text
- View/download PDF
4. Endogenous oxytocin exerts anti-nociceptive and anti-inflammatory effects in rats
- Author
-
Haruki Nishimura, Mitsuhiro Yoshimura, Makiko Shimizu, Kenya Sanada, Satomi Sonoda, Kazuaki Nishimura, Kazuhiko Baba, Naofumi Ikeda, Yasuhito Motojima, Takashi Maruyama, Yuki Nonaka, Ryoko Baba, Tatsushi Onaka, Takafumi Horishita, Hiroyuki Morimoto, Yasuhiro Yoshida, Makoto Kawasaki, Akinori Sakai, Masafumi Muratani, Becky Conway-Campbell, Stafford Lightman, and Yoichi Ueta
- Subjects
Biology (General) ,QH301-705.5 - Abstract
The analgesic effect of oxytocin appears to be mediated via an oxytocin receptor and endogenous oxytocin contributes to the anti-nociception and anti-inflammatory response.
- Published
- 2022
- Full Text
- View/download PDF
5. Diagnosis-specific Cumulative Incidence of Return-to-work, Resignation, and Death Among Long-term Sick-listed Employees: Findings From the Japan Epidemiology Collaboration on Occupational Health Study
- Author
-
Chihiro Nishiura, Yosuke Inoue, Ikuko Kashino, Akiko Nanri, Motoki Endo, Masafumi Eguchi, Takeshi Kochi, Noritada Kato, Makiko Shimizu, Teppei Imai, Akiko Nishihara, Makoto Yamamoto, Hiroko Okazaki, Kentaro Tomita, Toshiaki Miyamoto, Shuichiro Yamamoto, Tohru Nakagawa, Toru Honda, Takayuki Ogasawara, Naoko Sasaki, Ai Hori, Isamu Kabe, Tetsuya Mizoue, and Seitaro Dohi
- Subjects
mental disorders ,neoplasms ,retirement ,return to work ,sickness absence ,Medicine (General) ,R5-920 - Abstract
Background: While it is essential to understand how long is sufficient for return-to-work when designing paid sick-leave systems, little attempt has been done to collect cause-specific information on when and how many of sickness absentees returned to work, became unemployed, or passed away. Methods: We studied the first sick-leave episode of ≥30 consecutive days in those ≤55 years of age during 2012–2013 among employees of 11 Japanese private companies (n = 1,209), which were followed until 2017. Overall and disease-specific cumulative incidences of return-to-work, resignations, and deaths were estimated using competing risk analysis. Results: During the 3.5-year period (follow-up rate: 99.9%), 1,014 returned to work, 167 became unemployed, and 27 died. Overall, return-to-work occurred within 1 year in 74.9% of all absentees and in 89.3% of those who successfully returned to work. Resignation occurred within 1 year in 8.7% of all absentees and in 62.9% of all subjects who resigned. According to ICD-10 chapters, the cumulative incidence of return-to-work ranged from 82.1% for mental disorders (F00–F99) to 95.3% for circulatory diseases (I00–I99). The cumulative incidence of return-to-work due to mental disorders ranged from 66.7% in schizophrenia (F20) to 95.8% in bipolar affective disorders (F31). Death was rarely observed except for cases of neoplasms (C00–D48), of which the cumulative incidence of death reached 14.2% by 1.5 years. Conclusion: Return-to-work and resignations occurred commonly within 1 year of sick leave among long-term sickness absentees in the Japanese private companies. Our findings may assist occupational physicians and employers in developing effective social protection schemes.
- Published
- 2022
- Full Text
- View/download PDF
6. Plasma and synovial fluid concentrations of linezolid in patients with knee osteoarthritis infected with Staphylococcus aureus
- Author
-
Daisuke Negishi, Okimichi Mitsumatsu, Takahiro Matsumura, Hiromi Mitsumatsu, Miaki Makiguchi, Makiko Shimizu, and Hiroshi Yamazaki
- Subjects
Drug monitoring data ,Knee osteoarthritis ,Synovial fluid penetration ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background Linezolid is a new oxazolidinone antibiotic used for infections caused by methicillin-resistant Staphylococcus and other species. Case presentation Two cases of knee osteoarthritis with acute infection were successfully treated using linezolid. The plasma and synovial fluid concentrations of linezolid in two patients [women aged 69 and 73 years (cases 1 and 2)] with knee osteoarthritis infected with Staphylococcus aureus were measured after they were administered 600 mg twice daily by intravenous infusion. The plasma linezolid concentrations during knee surgery in case 1 at day 5 and in case 2 at day 2 were 19.6 and 15.6 μg/mL, respectively. The synovial fluid concentrations of linezolid in samples taken during surgery in case 1 and case 2 were 14.9 and 17.0 μg/mL, respectively; these values corresponded to ratios of synovial fluid/plasma of 76 and 109%. Possible metabolite 2-hydroxylated linezolid potentially mediated by cytochrome P450 2 J2 was not detected in the plasma or synovial fluid samples under the current clinical setting after multiple doses. Conclusions These results implied nearly equivalent concentrations of linezolid in plasma and synovial fluid of clinical patients with knee osteoarthritis acutely infected with Staphylococcus aureus.
- Published
- 2022
- Full Text
- View/download PDF
7. Pharmacokinetics of caffeine self-administered in overdose in a Japanese patient admitted to hospital
- Author
-
Koichiro Adachi, Satoru Beppu, Mariko Terashima, Toshiaki Fukuda, Jun Tomizawa, Makiko Shimizu, and Hiroshi Yamazaki
- Subjects
Pharmacokinetic modeling ,Overdose ,Serum potassium ,Paraxanthine ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background Caffeine (0.1 g) is used as a central nervous system stimulant and as a nontoxic phenotyping probe for cytochrome P450 1A2. However, an increasing number of suicide attempts by caffeine overdose have been recently reported. Case presentation A 25-year-old woman (body weight, 43 kg) who intentionally took an overdose of 5.9 g caffeine as a suicide attempt was emergently admitted to Kyoto Medical Center. The plasma concentrations of caffeine and its primary metabolite, N-demethylated paraxanthine, in the current case were 100 and 7.3 μg/mL, 81 and 9.9 μg/mL, 63 and 12 μg/mL, and 21 and 14 μg/mL, at 12, 20, 30, and 56 h after oral overdose, respectively. The observed apparent terminal elimination half-life of caffeine during days 1 and 2 of hospitalization was 27 h, which is several times longer than the reported normal value. This finding implied nonlinearity of caffeine pharmacokinetics over such a wide dose range, which could affect the accuracy of values simulated by a simplified physiologically based pharmacokinetic model founded on a normal dose of 100 mg. Low serum potassium levels (2.9 and 3.5 mM) on days 1 and 2 may have been caused by the caffeine overdose in the current case. Conclusions The patient underwent infusion with bicarbonate Ringer’s solution and potassium chloride and was discharged on the third day of hospitalization despite taking a potentially lethal dose of caffeine. The virtual plasma exposures of caffeine estimated using the current simplified PBPK model were higher than the measured values. The present results based on drug monitoring data and additional pharmacokinetic predictions could serve as a useful guide in cases of caffeine overdose.
- Published
- 2021
- Full Text
- View/download PDF
8. Pharmacokinetics of loxoprofen in a self-administered overdose in a Japanese patient admitted to hospital
- Author
-
Koichiro Adachi, Yuki Sugitani, Ryo Unita, Kosuke Yoshida, Satoru Beppu, Mariko Terashima, Masaya Fujii, Makiko Shimizu, and Hiroshi Yamazaki
- Subjects
Pharmacokinetic modeling ,Overdose ,Absorption ,Elimination ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background Loxoprofen is a propionic acid derivative and is the most widely prescribed non-steroidal anti-inflammatory drug in Japan. Loxoprofen is generally considered to be relatively nontoxic. Case presentation A 33-year-old man (body weight, 55 kg) who intentionally took an overdose of 100 tablets of loxoprofen (6000 mg) as a suicide attempt was emergently admitted to Kyoto Medical Center. On arrival, the patient was suffering disorders of consciousness. His plasma concentrations of loxoprofen and its reduced trans-alcohol metabolite were 52 and 24 μg/mL, 3.7 and 2.3 μg/mL, 0.81 and 0.54 μg/mL, and 0.015 and 0.011 μg/mL, respectively, at 4, 26, 50, and 121 h after the oral overdose. The observed apparent terminal elimination half-life of loxoprofen during days 1 and 2 of hospitalization was in the range 6–12 h, which is several times longer than the reported normal value. This finding implied nonlinearity of loxoprofen pharmacokinetics over the current 100-fold dose range, which could affect the accuracy of values simulated by a simplified physiologically based pharmacokinetic (PBPK) model founded on data from a normal dose of 60 mg. The reasons for the delayed eliminations from plasma of loxoprofen and its trans-alcohol metabolite in this case are uncertain, but slight renal impairment (low eGFR values) developed on the second and third hospital days and could be a causal factor. Conclusions Because the patient’s level of consciousness had gradually improved, he was discharged on the fourth day of hospitalization. The virtual plasma exposures of loxoprofen and its reduced trans-alcohol metabolite estimated using the current simplified PBPK model were lower than the measured values in the overdose case. The present results based on drug monitoring data and pharmacokinetic predictions could serve as a useful guide in cases of loxoprofen overdose.
- Published
- 2021
- Full Text
- View/download PDF
9. Pharmacokinetic modeling of over-the-counter drug diphenhydramine self-administered in overdoses in Japanese patients admitted to hospital
- Author
-
Koichiro Adachi, Satoru Beppu, Mariko Terashima, Wataru Kobari, Makiko Shimizu, and Hiroshi Yamazaki
- Subjects
Drug monitoring data ,Liver damage ,Pharmacokinetic prediction ,Total bilirubin ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background Although the over-the-counter H1 receptor antagonist diphenhydramine is not a common drug of abuse, it was recently recognized as one of the substances causing acute poisoning in patients attempting suicide that led to admissions to our hospital emergency room. Case presentation Two patients [women aged 21 and 27 years (cases 1 and 2)] were emergently admitted after intentionally taking overdoses of 900 and 1200 mg diphenhydramine, respectively. The plasma diphenhydramine concentrations in case 1 were 977 and 425 ng/mL at 2.5 and 11.5 h after single oral overdose, and those in case 2 were 1320 and 475 ng/mL at 3 and 18 h after administration, respectively. We set up a simplified physiologically based pharmacokinetic (PBPK) model that was established using the reported pharmacokinetic data for a microdose of diphenhydramine. The two virtual plasma concentrations and the area under the curve (AUC) values extrapolated using the PBPK model were consistent with the observed overdose data. This finding implied linearity of pharmacokinetics over a wide dosage range for diphenhydramine. Conclusions The determined plasma concentrations of diphenhydramine of around 1000 ng/mL at ~ 3 h after orally administered overdoses in cases 1 and 2 may not have been high enough to cause hepatic impairment because levels of aspartate aminotransferase and alanine aminotransferase were normal; however, there was an increase in total bilirubin in case 1. Nonetheless, high virtual liver exposures of diphenhydramine were estimated by the current PBPK model. The present results based on drug monitoring data and pharmacokinetic predictions could serve as a useful guide when setting the duration of treatment in cases of diphenhydramine overdose.
- Published
- 2021
- Full Text
- View/download PDF
10. Effects of polymorphic cytochrome P450 2A6 genotypes on chemoprevention against colorectal tumors in single Japanese cohort using daily low-dose aspirin: insights into future personalized treatments
- Author
-
Hiroshi Yamazaki, Makiko Shimizu, Takahiro Otani, Ami Mizugaki, Kanae Mure, Sadao Suzuki, and Hideki Ishikawa
- Subjects
CYP2A6 ,Acetylsalicylic acid ,Chemoprevention ,Ethnic difference ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background A chemopreventive effect of low-dose aspirin against colorectal tumors was previously found in participants of two Japanese multicenter, double-blind, randomized, placebo-controlled clinical trials investigating the effects of daily aspirin (100 mg/day) for 0.7–2 years on tumor recurrence in colorectal cancer patients whose tumors were excised endoscopically. Methods In the current study, chemopreventive data from single-center subsets having daily aspirin (100 mg/day) were reanalyzed with respect to variations in polymorphic cytochrome P450 2A6 (CYP2A6). From the J-CAPP study, 56 of 311 participants (47 men, 9 women; excluding patients with familial adenomatous polyposis) were genotyped for CYP2A6*1, *4 (whole-gene deletion), *7 (amino acid substitution), and *9 (upstream mutation), and from the J-FAPP IV study, 81 of 102 participants (43 men, 38 women; including patients with familial adenomatous polyposis) were also genotyped. Results The chemopreventive effects of daily aspirin were found to be inversely dependent on the predicted enzyme activity of the CYP2A6 phenotype [based on normal genotypes (CYP2A6*1/*1,*7,*9) and impaired genotypes (CYP2A6*4,*7,*9/*4,*7,*9 and CYP2A6*1/*4)] among a nonsmoker Japanese cohort without familial adenomatous polyposis. Conclusions The CYP2A6 wild-type allele could be a candidate biomarker for reduced chemopreventive effects of daily aspirin in a population with wide-ranging CYP2A6 phenotypes with a high frequency of impaired activities resulting from variations and whole-gene deletions. The CYP2A6 genotypes could be applicable to future personalized treatments for colorectal tumor chemoprevention with daily aspirin.
- Published
- 2021
- Full Text
- View/download PDF
11. Loss of Working Life Years Due to Mortality, Sickness Absence, or Ill-health Retirement: A Comprehensive Approach to Estimating Disease Burden in the Workplace
- Author
-
Yosuke Inoue, Shuhei Nomura, Chihiro Nishiura, Ai Hori, Kenya Yamamoto, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Masafumi Eguchi, Takeshi Kochi, Toshiaki Miyamoto, Hiroko Okazaki, Teppei Imai, Akiko Nishihara, Takayuki Ogasawara, Naoko Sasaki, Akihiko Uehara, Makoto Yamamoto, Makiko Shimizu, Maki Konishi, Isamu Kabe, Tetsuya Mizoue, and Seitaro Dohi
- Subjects
workplace ,occupational health ,sick leave ,retirement ,death ,Medicine (General) ,R5-920 - Abstract
Background: While much effort has focused on quantifying disease burden in occupational health, no study has simultaneously assessed disease burden in terms of mortality and morbidity. We aimed to propose a new comprehensive method of quantifying the disease burden in the workplace. Methods: The data were obtained from the Japan Epidemiology Collaboration on Occupational Health (J-ECOH) Study, a large-scale prospective study of approximately 80,000 workers. We defined disease burden in the workplace as the number of working years lost among the working population during a 6-year period (April 2012 to March 2018). We calculated the disease burden according to consequences of health problems (ie, mortality, sickness absence [SA], and ill-health retirement) and disease category. We also calculated the age-group- (20–39 and 40–59 years old) and sex-specific disease burden. Results: The largest contributors to disease burden in the workplace were mental and behavioural disorders (47.0 person-years lost per 10,000 person-years of working years; ie, per myriad [proportion]), followed by neoplasms (10.8 per myriad) and diseases of the circulatory system (7.1 per myriad). While mental and behavioural disorders made a greater contribution to SA and ill-health retirement compared to mortality, the latter two disorders were the largest contributors to the disease burden in the workplace due to mortality. The number of working years lost was greater among younger versus older female participants, whereas the opposite trend was observed in males. Conclusions: Our approach is in contrast to those in previous studies that focused exclusively on mortality or morbidity.
- Published
- 2021
- Full Text
- View/download PDF
12. Expression of oxytocin in hypothalamus and reduction of nociceptive stress following administration of Kamikihi-to in female rats
- Author
-
Takashi Maruyama, Makiko Shimizu, Naofumi Ikeda, Kazuhiko Baba, Mitsuhiro Yoshimura, and Yoichi Ueta
- Subjects
oxytocin ,nociceptive stress ,formalin test ,estrus cycle ,Kamikihi-to ,estrogen ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Hypothalamo-neurohypophysial oxytocin (OXT) plays an essential role in reproduction and in several socio-physiological functions, including stress reduction, anxiety relief, feeding suppression, social recognition, and trust building. Recent studies suggest that the central OXT system is also involved in antinociceptive and anti-inflammatory functions. Kamikihi-to (KKT), a Japanese traditional herbal (Kampo) medicine composed of 14 herbal ingredients, is clinically prescribed for patients with psychological symptoms, including anxiety, depression, and insomnia, and it has been associated with OXT expression. We investigated the antinociceptive response and OXT expression according to sex and the effects of KKT pre administration in a rat model. We found that nociceptive responses measured via the hot plate and formalin tests were attenuated following the administration of KKT-enriched feed for 4 weeks. The observation of mRFP1 fluorescence in OXT-mRFP1 transgenic rats revealed that KKT-administered rats showed increased expression of OXT in the magnocellular and parvocellular paraventricular nucleus of the hypothalamus. Food intake in the KKT-pre-administered group significantly decreased after cholecystokinin (CCK)-8 administration. Our results suggest that KKT is involved in the attenuation of nociceptive stress in female rats by enhancing the expression of OXT in the hypothalamus.
- Published
- 2022
- Full Text
- View/download PDF
13. Indication of intravaginal insemination for infertility treatment in couples with sexual dysfunction
- Author
-
Hiroshi Kaseki, Satoshi Kaseki, Makiko Shimizu, Ayako Hayashi, and Nobuhiko Suganuma
- Subjects
assisted reproductive technology ,erectile dysfunction ,infertility treatment ,intravaginal insemination ,sexual dysfunction ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Reproduction ,QH471-489 - Abstract
Abstract Purpose To analyze the usefulness of intravaginal insemination (IVI) for the infertility treatment in couples with sexual dysfunction before applying assisted reproductive technology (ART). Methods Among 208 couples who presented sexual dysfunction, 144 couples underwent IVI procedures. The profiles of pregnant and non‐pregnant patients were compared. Results Of 144 patients, 58 women conceived successfully (40.3% pregnancy rate). Between the pregnant and non‐pregnant cases, the husband's age and infertility period were significantly higher (P = .0104) and longer (P = .0027) in the unsuccessful cases than the successful ones. The husbands who could not impregnate had a significantly higher ratio of sperm abnormalities (P = .0048). Among the 57 successful cases who underwent IVI treatment, 38 (66.7%) patients became pregnant within 3 times of the procedure, while 48 (84.2%) patients conceived within 6 times. Conclusion The authors can propose the following inclusion IVI criteria for couples with sexual dysfunction: (a) younger husband (36 years old or less) which may be most important, (b) infertility duration of 3 years or less, (c) normal sperm condition, and (d) IVI trial for 3 times (maximum of 6 times). Since IVI appears to be a simple, noninvasive, and inexpensive way for couples with sexual dysfunction, it can be attempted before ART application.
- Published
- 2021
- Full Text
- View/download PDF
14. Pharmacokinetics of duloxetine self-administered in overdose with quetiapine and other antipsychotic drugs in a Japanese patient admitted to hospital
- Author
-
Koichiro Adachi, Satoru Beppu, Kei Nishiyama, Makiko Shimizu, and Hiroshi Yamazaki
- Subjects
Flunitrazepam ,Pharmacokinetic modeling ,Overdose ,Trazodone ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background Combinations of antidepressant duloxetine (at doses of 40–60 mg/day) and other antipsychotics are frequently used in clinical treatment; however, several fatal and nonfatal cases of duloxetine overdose have been documented. We experienced a patient who had taken an overdose of duloxetine (780 mg) in combination with other drugs in a suicide attempt. Case presentation The patient was a 37-year-old man (body weight, 64 kg) with a history of gender identity disorder and depression. He intentionally took an overdose of duloxetine in combination with three other antipsychotic drugs (18 mg flunitrazepam, 850 mg quetiapine, and 1100 mg trazodone) and was emergently admitted to Kyoto Medical Center. The patient’s plasma concentration of duloxetine during ambulance transport was 57 ng/ml, and the level was still as high as 126 ng/mL at 32 h after administration. Duloxetine disappeared most slowly from plasma, in contrast to quetiapine, which was the fastest to clear among the four medicines determined in this patient. The observed concentrations of duloxetine in this overdose patient were generally within the 95% confidence intervals of the plasma concentration curves predicted using a physiologically based pharmacokinetic (PBPK) model. Conclusion Even if more than 1 h (the generally recommended period) has passed after administration of duloxetine in such overdose cases, gastric lavage and/or administration of activated charcoal may be effective in clinical practice up to 6 h because of the typically slow elimination behavior illustrated by the PBPK model. Pharmacokinetic profiles visualized using PBPK modeling can inform treatment decisions in cases of drug overdose for medicines such as duloxetine in emergency clinical practice.
- Published
- 2021
- Full Text
- View/download PDF
15. Pharmacokinetics of anticoagulant edoxaban in overdose in a Japanese patient transported to hospital
- Author
-
Koichiro Adachi, Jumpei Tuchiya, Satoru Beppu, Kei Nishiyama, Makiko Shimizu, and Hiroshi Yamazaki
- Subjects
Anticoagulants ,Pharmacokinetic modeling ,Overdose ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Background The anticoagulant edoxaban is used clinically at doses of 30–60 mg/day; however, we experienced a patient who had taken an overdose of edoxaban of 750 mg. We investigated the pharmacokinetics of edoxaban in this patient by using liquid chromatography–tandem spectrometry to estimate the follow-up period in emergency clinical practice with this medicine. Case presentation The patient was a 57-year-old woman (body weight, 69 kg) who had taken a single oral dose of 750 mg of edoxaban in a suicide attempt. She was emergently admitted to Kyoto Medical Center. The patient’s edoxaban plasma concentrations during ambulance transport (8 h after oral administration) were ~ 4900 ng/ml, and the concentration gradually decreased to ~ 10 ng/mL and to detectable but unmeasurable levels of ~ 1.0 ng/mL at 60 h and 100 h, respectively. The linear range of the relationship between the dose and plasma concentration was assumed to have been exceeded during the first 8 h; however, the measured elimination rate of edoxaban was similar to that visualized curves predicted by a simplified physiologically based pharmacokinetic model previously established. Conclusion Simplified physiologically based pharmacokinetic models for creating visualized curves have proven to be useful not only during drug discovery or chemical risk assessment but also in cases of medical poisoning. We used a physiologically based pharmacokinetic model previously established for edoxaban to predict the pharmacokinetics in the current case. It is hoped that the results of this study, which encompass drug monitoring data in the patient and visualized pharmacokinetic prediction, will serve as an index when setting the treatment and follow-up period in cases of drug overdose for medicines such as edoxaban in emergency clinical practice.
- Published
- 2020
- Full Text
- View/download PDF
16. Associations of anemia and hemoglobin with hemoglobin A1c among non‐diabetic workers in Japan
- Author
-
Nobuaki Sakamoto, Huanhuan Hu, Akiko Nanri, Tetsuya Mizoue, Masafumi Eguchi, Takeshi Kochi, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Takayuki Ogasawara, Naoko Sasaki, Akiko Nishihara, Teppei Imai, Toshiaki Miyamoto, Makoto Yamamoto, Hiroko Okazaki, Kentaro Tomita, Akihiko Uehara, Ai Hori, Makiko Shimizu, Taizo Murakami, Keisuke Kuwahara, Ami Fukunaga, Isamu Kabe, Tomofumi Sone, and Seitaro Dohi
- Subjects
Anemia ,Hemoglobin ,Hemoglobin A1c ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Aims/Introduction We examined the association between hemoglobin A1c (HbA1c) and anemia, which was categorized into three groups according to mean corpuscular volume (MCV), as well as the association between hemoglobin in the non‐anemic range and HbA1c. Materials and Methods We used the 2016 health checkup data from 36,422 workers without diabetes. Anemic people were divided into three groups based on MCV: 90 fL. Non‐anemic people were divided into four groups based on their hemoglobin levels. We carried out multiple linear regression models to estimate the means and 95% confidence intervals (CIs) of HbA1c. Results For men, 0.2% had anemia with MCV 90 fL and 98.4% had no anemia. For women, the corresponding values were 6.1, 6.4, 2.8 and 84.7%, respectively. The adjusted mean HbA1c (%) values for men with anemia with MCV 90 fL were 5.67 (95% CI 5.60–5.74), 5.58 (95% CI 5.54–5.62) and 5.41 (95% CI 5.37–5.44), respectively. Among men without anemia, HbA1c (%) increased from 5.36 (95% CI 5.34–5.39) in those with hemoglobin ≥17.5 mg/dL to 5.45 (95% CI 5.45–5.46) in those with hemoglobin 13.0 to 90 fL, compared with non‐anemic men with hemoglobin 13.0 to 90 fL, suggesting that different types of anemia might influence HbA1c differently. In addition, non‐anemic people with lower hemoglobin levels had higher HbA1c levels, suggesting that hemoglobin levels are in need of consideration when interpreting HbA1c values among non‐anemic people.
- Published
- 2020
- Full Text
- View/download PDF
17. Determination and prediction of permeability across intestinal epithelial cell monolayer of a diverse range of industrial chemicals/drugs for estimation of oral absorption as a putative marker of hepatotoxicity
- Author
-
Yusuke Kamiya, Hiroka Takaku, Rio Yamada, Chisato Akase, Yuto Abe, Yuko Sekiguchi, Norie Murayama, Makiko Shimizu, Masato Kitajima, Fumiaki Shono, Kimito Funatsu, and Hiroshi Yamazaki
- Subjects
Caco-2 cells ,Octanol–water distribution coefficient ,Multivariate prediction equation ,Fraction absorbed ,No-observed-effect level ,Toxicology. Poisons ,RA1190-1270 - Abstract
Apparent permeability coefficients (Papp) across a human intestinal epithelial Caco-2 cell monolayer were measured for a range of industrial/drug chemicals. A predictive equation for determining in vitro Papp values of fifty-six substances was set up using multivariate regression analysis based on in silico-estimated physicochemical properties (molecular weights and water distribution coefficients for apical and basal pH environments) (r = 0.77, p
- Published
- 2020
- Full Text
- View/download PDF
18. Low serum creatinine and risk of diabetes: The Japan Epidemiology Collaboration on Occupational Health Study
- Author
-
Huanhuan Hu, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Hiroko Okazaki, Makoto Yamamoto, Toshiaki Miyamoto, Masafumi Eguchi, Takeshi Kochi, Makiko Shimizu, Taizo Murakami, Kentaro Tomita, Takayuki Ogasawara, Naoko Sasaki, Akihiko Uehara, Keisuke Kuwahara, Isamu Kabe, Tetsuya Mizoue, Tomofumi Sone, Seitaro Dohi, and the Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Diabetes ,Serum creatinine ,Skeletal muscle ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Aims/Introduction We examined a prospective association between serum creatinine levels and diabetes. Materials and Methods The present study included 31,343 male workers without diabetes, and aged between 20 and 64 years at baseline. We calculated the cumulative average of their serum creatinine over the study period. We defined diabetes as either glycated hemoglobin levels ≥6.5%, random glucose levels ≥200 mg/dL, fasting glucose levels ≥126 mg/dL or receiving antidiabetic treatment. Cox proportional hazards regression analysis was carried out to estimate the hazard ratio (HR) and 95% confidence interval (CI). Results With a median observation of 7.7 years, 2,509 participants developed diabetes. After adjusting for age, smoking, body mass index, hypertension and dyslipidemia, lower cumulative average serum creatinine levels were related to a greater diabetes risk: HRs were 1.56 (95% CI 1.35–1.82), 1.22 (1.09–1.35) and 1.06 (0.96–1.17) for the participants with serum creatinine
- Published
- 2019
- Full Text
- View/download PDF
19. Optimal waist circumference cut-off points and ability of different metabolic syndrome criteria for predicting diabetes in Japanese men and women: Japan Epidemiology Collaboration on Occupational Health Study
- Author
-
Huanhuan Hu, Kayo Kurotani, Naoko Sasaki, Taizo Murakami, Chii Shimizu, Makiko Shimizu, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Hiroko Okazaki, Satsue Nagahama, Akihiko Uehara, Makoto Yamamoto, Kentaro Tomita, Teppei Imai, Akiko Nishihara, Takeshi Kochi, Masafumi Eguchi, Toshiaki Miyamoto, Ai Hori, Keisuke Kuwahara, Shamima Akter, Ikuko Kashino, Isamu Kabe, Weiping Liu, Tetsuya Mizoue, Naoki Kunugita, Seitaro Dohi, and the Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Waist circumference ,Metabolic syndrome ,Diabetes mellitus ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background We sought to establish the optimal waist circumference (WC) cut-off point for predicting diabetes mellitus (DM) and to compare the predictive ability of the metabolic syndrome (MetS) criteria of the Joint Interim Statement (JIS) and the Japanese Committee of the Criteria for MetS (JCCMS) for DM in Japanese. Methods Participants of the Japan Epidemiology Collaboration on Occupational Health Study, who were aged 20–69 years and free of DM at baseline (n = 54,980), were followed-up for a maximum of 6 years. Time-dependent receiver operating characteristic analysis was used to determine the optimal cut-off points of WC for predicting DM. Time-dependent sensitivity, specificity, and positive and negative predictive values for the prediction of DM were compared between the JIS and JCCMS MetS criteria. Results During 234,926 person-years of follow-up, 3180 individuals developed DM. Receiver operating characteristic analysis suggested that the most suitable cut-off point of WC for predicting incident DM was 85 cm for men and 80 cm for women. MetS was associated with 3–4 times increased hazard for developing DM in men and 7–9 times in women. Of the MetS criteria tested, the JIS criteria using our proposed WC cut-off points (85 cm for men and 80 cm for women) had the highest sensitivity (54.5 % for men and 43.5 % for women) for predicting DM. The sensitivity and specificity of the JCCMS MetS criteria were ~37.7 and 98.9 %, respectively. Conclusion Data from the present large cohort of workers suggest that WC cut-offs of 85 cm for men and 80 cm for women may be appropriate for predicting DM for Japanese. The JIS criteria can detect more people who later develop DM than does the JCCMS criteria.
- Published
- 2016
- Full Text
- View/download PDF
20. Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
- Author
-
Makiko Shimizu, Tomoki Koyama, Izumi Kishimoto, and Hiroshi Yamazaki
- Subjects
CYP2A6*1 ,CYP2A6*4 ,Copy number assays ,Real-time PCR ,TaqMan ,Whole-gene deletion ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Science (General) ,Q1-390 - Abstract
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes” (Shimizu et al., Clinica Chimica Acta 441, 71–74, 2015), which presents a multiplex real-time polymerase chain reaction method with dual-labeled probes for human P450 2A6 wild-type and whole-gene deletion. Real-time methods have dramatically improved the speed of complex genetic diagnostics compared to conventional assays based on restriction enzyme digestion. Here, we show the basic assay validation data by single and multiplex determinations in comparison with commercial TaqMan copy number assays for P450 2A6.
- Published
- 2015
- Full Text
- View/download PDF
21. Analysis of six novel flavin-containing monooxygenase 3 (FMO3) gene variants found in a Japanese population suffering from trimethylaminuria
- Author
-
Makiko Shimizu, Yumi Origuchi, Marika Ikuma, Nanako Mitsuhashi, and Hiroshi Yamazaki
- Subjects
Flavin-containing monooxygenase 3 ,Fish odor syndrome ,Trimethylamine ,Polymorphism ,Japanese ,Trimethylaminuria ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Polymorphic human flavin-containing monooxygenase 3 (FMO3) is associated with the inherited disorder trimethylaminuria. Several FMO3 variants have been observed in a variety of ethnic groups, including a Japanese cohort suffering from trimethylaminuria. The aim of this study was to screen another self-reported Japanese trimethylaminuria cohort for novel FMO3 variants and to investigate these new variants. Subjects with low FMO3 metabolic capacities were identified by measuring the urinary trimethylamine and trimethylamine N-oxide concentrationsin171 Japanese volunteers. The FMO3 genes from these subjects and their family members were then sequenced. Heterozygotes or homozygotes for novel single-nucleotide polymorphisms c.20 T>C p.(Ile7Thr), c.122 G>A p.(Trp41Ter), c.127T>A p.(Phe43Ile), c.488 T>C p.(Leu163Pro), and c.1127G>A p.(Gly376Glu) and a heterozygote for the novel duplication c.850_860dupTTTAACGATGA p.(Glu287AspfsTer17) were identified. In addition, the known (but as yet uncharacterized) single-nucleotide polymorphism c.929 C>T p.(Ser310Leu) was found. Pedigree analysis revealed the p.(Ser310Leu) FMO3 allele in cis configuration with c.929 C>T p.(Glu158Lys). These variant FMO3 proteins recombinantly expressed in Escherichia coli membranes exhibited decreased N-oxygenation activities toward trimethylamine and benzydamine. Although the allele frequencies of these seven variants were low, the present results suggest that individuals homozygous or heterozygous for any of these novel missense or duplicationFMO3 variants or known nonsense mutations such as p.(Cys197Ter) may possess abnormal activities toward trimethylamine N-oxygenation.
- Published
- 2015
- Full Text
- View/download PDF
22. Preclinical and phase I clinical studies of KW-2450, a dual IGF-1R/IR tyrosine kinase inhibitor, in combination with lapatinib and letrozole
- Author
-
Hiroshi Umehara, Yoshimi Maekawa, Fumito Koizumi, Makiko Shimizu, Toshio Ota, Tamer M. Fouad, Jie Willey, Hidekuni Kaito, Norihiko Shiraishi, Daisuke Nakashima, Shiro Akinaga, and Naoto T. Ueno
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: KW-2450 is an oral dual insulin-like growth factor-1 receptor/insulin receptor tyrosine kinase inhibitor. We investigated the in vitro and in vivo preclinical activity of KW-2450 plus lapatinib and letrozole and conducted a phase I trial of the triple-drug combination in one male and 10 postmenopausal female patients with advanced/metastatic hormone receptor-positive, human epidermal growth factor receptor 2 (HER2)-positive breast cancer. Methods: A series of in vitro and in vivo animal studies was undertaken of KW-2450 in combination with lapatinib and hormonal agents. The phase I trial was conducted to establish the safety, tolerability, and recommended phase II dose (RP2D) of KW-2450 administered in combination with lapatinib and letrozole. Results: Preclinical studies showed KW-2450 and lapatinib act synergistically to induce in vitro apoptosis and inhibit growth of HER2-positive MDA-MB-361 and BT-474 breast cancer cell lines. This combined effect was confirmed in vivo using the MDA-MB-361 xenograft model. KW-2450 showed synergistic in vitro growth inhibition with letrozole and 4-hydroxytamoxifen in ER-positive MCF-7 breast cancer cells and MCF-7-Ac1 aromatase-transfected MCF-7 cells. In the phase I study, dose-limiting toxicity (DLT; grade 3 rash and grade 3 hyperglycemia, respectively) occurred in two of three patients at the dose of KW-2450 25 mg/day plus lapatinib 1500 mg/day and letrozole 2.5 mg/day. The RP2D of the triple-drug combination was established as KW-2450 25 mg/day, lapatinib 1250 mg/day, and letrozole 2.5 mg/day with no DLT at this dose level. Conclusions: The proposed phase II study of the RP2D for the triple-drug combination did not progress because of anticipated difficulty in patient enrollment and further clinical development of KW-2450 was terminated.
- Published
- 2018
- Full Text
- View/download PDF
23. Correction: Development of Risk Score for Predicting 3-Year Incidence of Type 2 Diabetes: Japan Epidemiology Collaboration on Occupational Health Study.
- Author
-
Akiko Nanri, Tohru Nakagawa, Keisuke Kuwahara, Shuichiro Yamamoto, Toru Honda, Hiroko Okazaki, Akihiko Uehara, Makoto Yamamoto, Toshiaki Miyamoto, Takeshi Kochi, Masafumi Eguchi, Taizo Murakami, Chii Shimizu, Makiko Shimizu, Kentaro Tomita, Satsue Nagahama, Teppei Imai, Akiko Nishihara, Naoko Sasaki, Ai Hori, Nobuaki Sakamoto, Chihiro Nishiura, Takafumi Totsuzaki, Noritada Kato, Kenji Fukasawa, Huanhuan Hu, Shamima Akter, Kayo Kurotani, Isamu Kabe, Tetsuya Mizoue, Tomofumi Sone, Seitaro Dohi, and Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0142779.].
- Published
- 2018
- Full Text
- View/download PDF
24. Hba1c, Blood Pressure, and Lipid Control in People with Diabetes: Japan Epidemiology Collaboration on Occupational Health Study.
- Author
-
Huanhuan Hu, Ai Hori, Chihiro Nishiura, Naoko Sasaki, Hiroko Okazaki, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Kentaro Tomita, Toshiaki Miyamoto, Satsue Nagahama, Akihiko Uehara, Makoto Yamamoto, Taizo Murakami, Chii Shimizu, Makiko Shimizu, Masafumi Eguchi, Takeshi Kochi, Teppei Imai, Akiko Okino, Keisuke Kuwahara, Ikuko Kashino, Shamima Akter, Kayo Kurotani, Akiko Nanri, Isamu Kabe, Tetsuya Mizoue, Naoki Kunugita, Seitaro Dohi, and Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Medicine ,Science - Abstract
The control of blood glucose levels, blood pressure (BP), and low-density lipoprotein cholesterol (LDL-C) levels reduces the risk of diabetes complications; however, data are scarce on control status of these factors among workers with diabetes. The present study aimed to estimate the prevalence of participants with diabetes who meet glycated hemoglobin (HbA1c), BP, and LDL-C recommendations, and to investigate correlates of poor glycemic control in a large working population in Japan.The Japan Epidemiology Collaboration on Occupational Health (J-ECOH) Study is an ongoing cohort investigation, consisting mainly of employees in large manufacturing companies. We conducted a cross-sectional analysis of 3,070 employees with diabetes (2,854 men and 216 women) aged 20-69 years who attended periodic health examinations. BP was measured and recorded using different company protocols. Risk factor targets were defined using both American Diabetes Association (ADA) guidelines (HbA1c < 7.0%, BP < 140/90 mmHg, and LDL-C < 100 mg/dL) and Japan Diabetes Society (JDS) guidelines (HbA1c < 7.0%, BP < 130/80 mmHg, and LDL-C < 120 mg/dL). Logistic regression models were used to explore correlates of poor glycemic control (defined as HbA1c ≥ 8.0%).The percentages of participants who met ADA (and JDS) targets were 44.9% (44.9%) for HbA1c, 76.6% (36.3%) for BP, 27.1% (56.2%) for LDL-C, and 11.2% (10.8%) for simultaneous control of all three risk factors. Younger age, obesity, smoking, and uncontrolled dyslipidemia were associated with poor glycemic control. The adjusted odds ratio of poor glycemic control was 0.58 (95% confidence interval, 0.46-0.73) for participants with treated but uncontrolled hypertension, and 0.47 (0.33-0.66) for participants with treated and controlled hypertension, as compared with participants without hypertension. There was no significant difference in HbA1c levels between participants with treated but uncontrolled hypertension and those with treated and controlled hypertension.Data from a large working population, predominantly composed of men, suggest that achievement of HbA1c, BP, and LDL-C targets was less than optimal, especially in younger participants. Uncontrolled dyslipidemia was associated with poor glycemic control. Participants not receiving antihypertensive treatment had higher HbA1c levels.
- Published
- 2016
- Full Text
- View/download PDF
25. Correction: Smoking, Smoking Cessation, and the Risk of Type 2 Diabetes among Japanese Adults: Japan Epidemiology Collaboration on Occupational Health Study.
- Author
-
Shamima Akter, Hiroko Okazaki, Keisuke Kuwahara, Toshiaki Miyamoto, Taizo Murakami, Chii Shimizu, Makiko Shimizu, Kentaro Tomita, Satsue Nagahama, Masafumi Eguchi, Takeshi Kochi, Teppei Imai, Akiko Nishihara, Naoko Sasaki, Tohru Nakagawa, Shuichiro Yamamoto, Toru Honda, Akihiko Uehara, Makoto Yamamoto, Ai Hori, Nobuaki Sakamoto, Chihiro Nishiura, Takafumi Totsuzaki, Noritada Kato, Kenji Fukasawa, Ngoc M Pham, Kayo Kurotani, Akiko Nanri, Isamu Kabe, Tetsuya Mizoue, Tomofumi Sone, Seitaro Dohi, and Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Medicine ,Science - Published
- 2015
- Full Text
- View/download PDF
26. Smoking, Smoking Cessation, and the Risk of Type 2 Diabetes among Japanese Adults: Japan Epidemiology Collaboration on Occupational Health Study.
- Author
-
Shamima Akter, Hiroko Okazaki, Keisuke Kuwahara, Toshiaki Miyamoto, Taizo Murakami, Chii Shimizu, Makiko Shimizu, Kentaro Tomita, Satsue Nagahama, Masafumi Eguchi, Takeshi Kochi, Teppei Imai, Akiko Nishihara, Naoko Sasaki, Tohru Nakagawa, Shuichiro Yamamoto, Toru Honda, Akihiko Uehara, Makoto Yamamoto, Ai Hori, Nobuaki Sakamoto, Chihiro Nishiura, Takafumi Totsuzaki, Noritada Kato, Kenji Fukasawa, Ngoc M Pham, Kayo Kurotani, Akiko Nanri, Isamu Kabe, Tetsuya Mizoue, Tomofumi Sone, Seitaro Dohi, and Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Medicine ,Science - Abstract
To examine the association of smoking status, smoking intensity, and smoking cessation with the risk of type 2 diabetes (T2D) using a large database.The present study included 53,930 Japanese employees, aged 15 to 83 years, who received health check-up and did not have diabetes at baseline. Diabetes was defined as fasting plasma glucose ≥126 mg/dl, random plasma glucose ≥200 mg/dl, HbA1c ≥6.5% (≥48 mmol/mol), or receiving medication for diabetes. Cox proportional-hazards regression models were used to investigate the association between smoking and the risk of diabetes.During 3.9 years of median follow-up, 2,441 (4.5%) individuals developed T2D. The multivariable-adjusted hazard ratios (95% CI) for diabetes were 1 (reference), 1.16 (1.04 to 1.30) and 1.34 (1.22 to 1.48) for never smokers, former smokers, and current smokers, respectively. Diabetes risk increased with increasing numbers of cigarette consumption among current smokers (P for trend
- Published
- 2015
- Full Text
- View/download PDF
27. Development of Risk Score for Predicting 3-Year Incidence of Type 2 Diabetes: Japan Epidemiology Collaboration on Occupational Health Study.
- Author
-
Akiko Nanri, Tohru Nakagawa, Keisuke Kuwahara, Shuichiro Yamamoto, Toru Honda, Hiroko Okazaki, Akihiko Uehara, Makoto Yamamoto, Toshiaki Miyamoto, Takeshi Kochi, Masafumi Eguchi, Taizo Murakami, Chii Shimizu, Makiko Shimizu, Kentaro Tomita, Satsue Nagahama, Teppei Imai, Akiko Nishihara, Naoko Sasaki, Ai Hori, Nobuaki Sakamoto, Chihiro Nishiura, Takafumi Totsuzaki, Noritada Kato, Kenji Fukasawa, Hu Huanhuan, Shamima Akter, Kayo Kurotani, Isamu Kabe, Tetsuya Mizoue, Tomofumi Sone, Seitaro Dohi, and Japan Epidemiology Collaboration on Occupational Health Study Group
- Subjects
Medicine ,Science - Abstract
Risk models and scores have been developed to predict incidence of type 2 diabetes in Western populations, but their performance may differ when applied to non-Western populations. We developed and validated a risk score for predicting 3-year incidence of type 2 diabetes in a Japanese population.Participants were 37,416 men and women, aged 30 or older, who received periodic health checkup in 2008-2009 in eight companies. Diabetes was defined as fasting plasma glucose (FPG) ≥ 126 mg/dl, random plasma glucose ≥ 200 mg/dl, glycated hemoglobin (HbA1c) ≥ 6.5%, or receiving medical treatment for diabetes. Risk scores on non-invasive and invasive models including FPG and HbA1c were developed using logistic regression in a derivation cohort and validated in the remaining cohort.The area under the curve (AUC) for the non-invasive model including age, sex, body mass index, waist circumference, hypertension, and smoking status was 0.717 (95% CI, 0.703-0.731). In the invasive model in which both FPG and HbA1c were added to the non-invasive model, AUC was increased to 0.893 (95% CI, 0.883-0.902). When the risk scores were applied to the validation cohort, AUCs (95% CI) for the non-invasive and invasive model were 0.734 (0.715-0.753) and 0.882 (0.868-0.895), respectively. Participants with a non-invasive score of ≥ 15 and invasive score of ≥ 19 were projected to have >20% and >50% risk, respectively, of developing type 2 diabetes within 3 years.The simple risk score of the non-invasive model might be useful for predicting incident type 2 diabetes, and its predictive performance may be markedly improved by incorporating FPG and HbA1c.
- Published
- 2015
- Full Text
- View/download PDF
28. Qualitative de novo analysis of full length cDNA and quantitative analysis of gene expression for common marmoset (Callithrix jacchus) transcriptomes using parallel long-read technology and short-read sequencing.
- Author
-
Makiko Shimizu, Shunsuke Iwano, Yasuhiro Uno, Shotaro Uehara, Takashi Inoue, Norie Murayama, Jun Onodera, Erika Sasaki, and Hiroshi Yamazaki
- Subjects
Medicine ,Science - Abstract
The common marmoset (Callithrix jacchus) is a non-human primate that could prove useful as human pharmacokinetic and biomedical research models. The cytochromes P450 (P450s) are a superfamily of enzymes that have critical roles in drug metabolism and disposition via monooxygenation of a broad range of xenobiotics; however, information on some marmoset P450s is currently limited. Therefore, identification and quantitative analysis of tissue-specific mRNA transcripts, including those of P450s and flavin-containing monooxygenases (FMO, another monooxygenase family), need to be carried out in detail before the marmoset can be used as an animal model in drug development. De novo assembly and expression analysis of marmoset transcripts were conducted with pooled liver, intestine, kidney, and brain samples from three male and three female marmosets. After unique sequences were automatically aligned by assembling software, the mean contig length was 718 bp (with a standard deviation of 457 bp) among a total of 47,883 transcripts. Approximately 30% of the total transcripts were matched to known marmoset sequences. Gene expression in 18 marmoset P450- and 4 FMO-like genes displayed some tissue-specific patterns. Of these, the three most highly expressed in marmoset liver were P450 2D-, 2E-, and 3A-like genes. In extrahepatic tissues, including brain, gene expressions of these monooxygenases were lower than those in liver, although P450 3A4 (previously P450 3A21) in intestine and P450 4A11- and FMO1-like genes in kidney were relatively highly expressed. By means of massive parallel long-read sequencing and short-read technology applied to marmoset liver, intestine, kidney, and brain, the combined next-generation sequencing analyses reported here were able to identify novel marmoset drug-metabolizing P450 transcripts that have until now been little reported. These results provide a foundation for mechanistic studies and pave the way for the use of marmosets as model animals for drug development in the future.
- Published
- 2014
- Full Text
- View/download PDF
29. Octanol/water partition coefficients estimated using retention times in reverse-phase liquid chromatography and calculated in silico as one of the determinant factors for pharmacokinetic parameter estimations of general chemical substances.
- Author
-
Koichiro Adachi, Makiko Shimizu, Fumiaki Shono, Kimito Funatsu, and Hiroshi Yamazaki
- Subjects
- *
LIQUID chromatography , *PARAMETER estimation , *OCTYL alcohol , *PHARMACOKINETICS , *TIME management , *PARTITION coefficient (Chemistry) , *RF values (Chromatography) - Abstract
The octanol/water partition coefficient P (logP) is a hydrophobicity index and is one of the determining factors for the pharmacokinetics of orally administered substances because it influences membrane permeability. To illustrate the wide-ranging variety of compounds in the chemical space, a two-dimensional data plot consisting of 25 blocks was previously proposed based on a substance’s in silico chemical descriptors. The logP values of approximately 200 diverse chemicals (test plus reference compounds covering all 25 blocks of the chemical space) were estimated experimentally using retention times in reverse-phase liquid chromatography; these values were compared with those of authentic reference compounds with established logP values (available for 17 of 60 reference substances in the Organization for Economic Co-operation and Development Test Guideline 117). The logP values of 140 of 165 chemicals successfully estimated using four different mobile phase conditions (pH 2, 4, 7, and 10 for molecular forms) correlated significantly with those calculated using the in silico packages ChemDraw and ACD/Percepta (r > 0.72). Although substances that neighbored authentic compounds in the chemical space had precisely correlated logP values estimated experimentally and in silico, some compounds that were more distant from authentic substances showed lower logP values than those estimated in silico. These results indicate that additional authentic reference materials with wider ranging chemical diversity and their logP values from reverse-phase liquid chromatography should be included in the international test guidance to promote simple and reliable estimation of octanol/water partition coefficients, which are important determinant factors for the pharmacokinetics of general chemicals. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
30. Plasma and Hepatic Exposures of Celecoxib and Diclofenac Prescribed Alone in Patients with Cytochrome P450 2C9*3 Modeled after Virtual Oral Administrations and Likely Associated with Adverse Drug Events Reported in a Japanese Database
- Author
-
Koichiro Adachi, Katsuhiro Ohyama, Yoichi Tanaka, Hina Nakano, Tasuku Sato, Norie Murayama, Makiko Shimizu, Yoshiro Saito, and Hiroshi Yamazaki
- Subjects
Pharmacology ,Pharmaceutical Science ,General Medicine - Published
- 2023
31. Updated in Silico Prediction Methods for Fractions Absorbed and Key Input Parameters of 355 Disparate Chemicals for Physiologically Based Pharmacokinetic Models for Time-Dependent Plasma Concentrations after Virtual Oral Doses in Humans
- Author
-
Koichiro, Adachi, Makiko, Shimizu, and Hiroshi, Yamazaki
- Subjects
Pharmacology ,Humans ,Animals ,Administration, Oral ,Pharmaceutical Science ,Computer Simulation ,General Medicine ,Models, Biological ,Rats - Abstract
Human metabolic profiles for substances such as toxic food-derived compounds are usually allometrically extrapolated from traditionally determined in vivo rat concentration profiles. To evaluate internal exposures in humans without any reference to experimental data, physiologically based pharmacokinetic (PBPK) modeling could be used if the model input parameters could be estimated in silico. This approach would simplify the use of PBPK models for forward dosimetry after oral doses. In this study, the in silico estimation of input parameters for PBPK models (i.e., fraction absorbed × intestinal availability, absorption rate constants, and volumes of the systemic circulation) was updated for an panel of 355 chemicals (212 previously analyzed and 143 additional substances) using a light gradient boosting machine learning algorithms (LightGBM) based on between 11 and 29 in silico-calculated chemical descriptors. Simplified human PBPK models were then used to calculate virtual maximum plasma concentrations (C
- Published
- 2022
32. Diagnosis-specific Cumulative Incidence of Return-to-work, Resignation, and Death Among Long-term Sick-listed Employees: Findings From the Japan Epidemiology Collaboration on Occupational Health Study
- Author
-
Toru Honda, Makoto Yamamoto, Naoko Sasaki, Kentaro Tomita, Isamu Kabe, Teppei Imai, Hiroko Okazaki, Masafumi Eguchi, Noritada Kato, Akiko Nishihara, Tetsuya Mizoue, Toshiaki Miyamoto, Seitaro Dohi, Ai Hori, Yosuke Inoue, Shuichiro Yamamoto, Takeshi Kochi, Takayuki Ogasawara, Akiko Nanri, Makiko Shimizu, Chihiro Nishiura, Tohru Nakagawa, Motoki Endo, and Ikuko Kashino
- Subjects
medicine.medical_specialty ,Epidemiology ,030209 endocrinology & metabolism ,Occupational physicians ,Competing risks ,Return to work ,Occupational safety and health ,03 medical and health sciences ,Return to Work ,0302 clinical medicine ,Japan ,Humans ,Medicine ,Cumulative incidence ,030212 general & internal medicine ,Occupational Health ,Sickness absence ,business.industry ,Incidence ,Mental Disorders ,General Medicine ,Sick leave ,Sick Leave ,business ,Demography - Abstract
Background While it is essential to understand how long is sufficient for return-to-work when designing paid sick-leave systems, little attempt has been done to collect cause-specific information on when and how many of sickness absentees returned to work, became unemployed, or passed away. Methods We studied the first sick-leave episode of ≥30 consecutive days in those ≤55 years of age during 2012-2013 among 11 Japanese private companies (n = 1209), which were followed until 2017. Overall and disease-specific cumulative incidences of return-to-work, resignations, and deaths were estimated using competing risk analysis. Results During the 3.5-year period (follow-up rate: 99.9%), 1014 returned to work, 167 became unemployed, and 27 died. Overall, return-to-work occurred within 1 year in 74.9% of all absentees and in 89.3% of those who successfully returned to work. Resignation occurred within 1 year in 8.7% of all absentees and in 62.9% of all subjects who resigned. According to ICD-10 chapters, the cumulative incidence of return-to-work ranged from 82.1% for mental disorders (F00-F99) to 95.3% for circulatory diseases (I00-I99). The cumulative incidence of return-to-work due to mental disorders ranged from 66.7% in schizophrenia (F20) to 95.8% in bipolar affective disorders (F31). Death was rarely observed except for cases of neoplasms (C00-D48), of which the cumulative incidence of death reached 14.2% by 1.5 years. Conclusions Return-to-work and resignations occurred commonly within 1 year of sick leave among long-term sickness absentees in the Japanese private companies. Our findings may assist occupational physicians and employers in developing effective social protection schemes.
- Published
- 2022
33. Species Specificity and Selection of Models for Drug Oxidations Mediated by Polymorphic Human Enzymes
- Author
-
Hiroshi Yamazaki and Makiko Shimizu
- Subjects
Pharmacology ,Pharmaceutical Science - Published
- 2022
34. Plasma Concentration Profiles for Hepatotoxic Pyrrolizidine Alkaloid Senkirkine in Humans Extrapolated from Rat Data Sets Using a Simplified Physiologically Based Pharmacokinetic Model
- Author
-
Hiroshi Yamazaki, Yusuke Kamiya, Tomonori Miura, Airi Kato, Norie Murayama, and Makiko Shimizu
- Subjects
Drug-Related Side Effects and Adverse Reactions ,Animals ,Humans ,Water ,Chemical and Drug Induced Liver Injury ,Pyrrolizidine Alkaloids ,Rats - Abstract
Aim: The main aim of the current study was to obtain forward dosimetry assessments of pyrrolizidine alkaloid senkirkine plasma and liver concentrations by setting up a human physiologically based pharmacokinetic (PBPK) model based on the limited information available. Background: The risks associated with plant-derived pyrrolizidine alkaloids as natural toxins have been assessed. Objective: The pyrrolizidine alkaloid senkirkine was investigated because it was analyzed in a European transcriptomics study of natural hepatotoxins and in a study of the alkaloidal constituents of traditional Japanese food plants Petasites japonicus. The in silico human plasma and liver concentrations of senkirkine were modeled using doses reported for acute-term toxicity in humans. Methods: Using a simplified PBPK model established using rat pharmacokinetic data, forward dosimetry was conducted. Since in vitro rat and human intrinsic hepatic clearances were similar; an allometric scaling approach was applied to rat parameters to create a human PBPK model. Results: After oral administration of 1.0 mg/kg in rats in vivo, water-soluble senkirkine was absorbed and cleared from plasma to two orders of magnitude below the maximum concentration in 8 h. Human in silico senkirkine plasma concentration curves were generated after virtual daily oral administrations of 3.0 mg/kg senkirkine (the dose involved in an acute fatal hepatotoxicity case). A high concentration of senkirkine in the culture medium caused in vitro hepatotoxicity as evidenced by lactate dehydrogenase leakage from human hepatocyte-like HepaRG cells. Conclusion: Higher virtual concentrations of senkirkine in human liver and plasma than those in rat plasma were estimated using the current rat and human PBPK models. Current simulations suggest that if P. japonicus (a water-soluble pyrrolizidine alkaloid-producing plant) is ingested daily as food, hepatotoxic senkirkine could be continuously present in human plasma and liver.
- Published
- 2022
35. Updated in silico prediction methods for fractions absorbed and absorption rate constants of 372 disparate chemicals for use in physiologically based pharmacokinetic models for estimating internal concentrations in rats
- Author
-
Koichiro, Adachi, Makiko, Shimizu, and Hiroshi, Yamazaki
- Subjects
Humans ,Animals ,Toxicology ,Models, Biological ,Rats - Abstract
Physiologically based pharmacokinetic (PBPK) modeling has the potential to estimate internal chemical exposures. Algorithms for predicting the input parameters for PBPK modeling, such as absorption rate constants (k
- Published
- 2022
36. Réalité et transformation des « pratiques de relations commerciales japonaises » : le cas de l’architecture modulaire
- Author
-
Makiko Shimizu, Simon Serverin, and Gakuto Takamura
- Subjects
Sociology and Political Science ,Law - Published
- 2021
37. Differences in Pharmacokinetics and Haematotoxicities of Aniline and Its Dimethyl Derivatives Orally Administered in Rats
- Author
-
Yusuke Kamiya, Makiko Shimizu, Norie Murayama, Tomonori Miura, and Hiroshi Yamazaki
- Subjects
Male ,Pharmacology ,Aniline Compounds ,Chromatography ,Hemolytic Agents ,Chemistry ,Administration, Oral ,Pharmaceutical Science ,General Medicine ,Hydroxylation ,Positive correlation ,Mass spectrometry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Aniline ,Pharmacokinetics ,Acetylation ,Area Under Curve ,Plasma concentration ,Animals ,Methyl group ,N hydroxylation - Abstract
Aniline and its dimethyl derivatives reportedly become haematotoxic after metabolic N-hydroxylation of their amino groups. The plasma concentrations of aniline and its dimethyl derivatives after single oral doses of 25 mg/kg in rats were quantitatively measured and semi-quantitatively estimated using liquid chromatography-tandem mass spectrometry. The quantitatively determined elimination rates of aniline; 2,4-dimethylaniline; and 3,5-dimethylaniline based on rat plasma versus time curves were generally rapid compared with those of 2,3-; 2,5-; 2,6-; and N,2-dimethylaniline. The primary acetylated metabolites of aniline; 2,4-dimethylaniline; and 3,5-dimethylaniline, as semi-quantitatively estimated based on their peak areas in liquid chromatography analyses, were more extensively formed than those of 2,3-; 2,5-; 2,6-; and N,2-dimethylaniline. The areas under the curve of unmetabolized (remaining) aniline and its dimethyl derivatives estimated using simplified physiologically based pharmacokinetic models (that were set up using the experimental plasma concentrations) showed an apparently positive correlation with the reported lowest-observed-effect levels for haematotoxicity of these chemicals. In the case of 2,4-dimethylaniline, a methyl group at another C4-positon would be one of the determinant factors for rapid metabolic elimination to form aminotoluic acid. These results suggest that rapid and extensive metabolic activation of aniline and its dimethyl derivatives occurred in rats and that the presence of a methyl group at the C2-positon may generally suppress fast metabolic rates of dimethyl aniline derivatives that promote metabolic activation reactions at NH2 moieties.
- Published
- 2021
38. Forward and reverse dosimetry for aniline and 2,6-dimethylaniline in humans extrapolated from humanized-liver mouse data using simplified physiologically based pharmacokinetic models
- Author
-
Tomonori Miura, Shotaro Uehara, Makiko Shimizu, Hiroshi Suemizu, and Hiroshi Yamazaki
- Subjects
Mice ,Aniline Compounds ,Liver ,Humans ,Animals ,Epichlorohydrin ,Toxicology ,Rats - Abstract
Although human urinary aniline and 2,6-dimethylaniline were unexpectedly detected in biomonitoring data, little is known about the daily intake doses of aniline and 2,6-dimethylaniline in the living environment or their relation to tolerable daily intake (TDI) values in humans. In the current study, to evaluate the daily oral intake of aniline and 2,6-dimethylaniline in humans, forward and reverse dosimetry was carried out using simplified in silico physiologically based pharmacokinetic (PBPK) modeling established using in vivo experimental pharmacokinetic data. These data were from humanized-liver mice after single oral doses of 100 mg/kg aniline (previously determined) and 116 mg/kg 2,6-dimethylanine (currently investigated). The in vivo elimination rates of 2,6-dimethylaniline from plasma in humanized-liver mice were generally slow compared with those of aniline. Faster in vitro metabolic elimination rates of aniline mediated by liver 9000 × g supernatant fractions from rats than those from humans may suggest the existence of higher first-pass effects in rats than in humanized-liver mice. In silico aniline and 2,6-dimethylaniline concentration curves in human urine after virtual oral administrations were estimated by human PBPK models created with data from humanized-liver mice. Reverse dosimetry analysis using human PBPK models estimated the daily intake of aniline, based on reported human urinary concentrations in biomonitoring data, to be roughly similar to the aniline TDI level. These results suggest that forward and reverse dosimetry using simplified human PBPK models founded on data from humanized-liver mice can be used to evaluate possible higher than expected exposures of aniline and 2,6-dimethylaniline in humans.
- Published
- 2022
39. An Updated In Silico Prediction Method for Volumes of Systemic Circulation of 323 Disparate Chemicals for Use in Physiologically Based Pharmacokinetic Models to Estimate Plasma and Tissue Concentrations after Oral Doses in Rats
- Author
-
Yusuke Kamiya, Junya Ohori, Fumiaki Shono, Hiroshi Yamazaki, Kimito Funatsu, Tomonori Miura, Masato Kitajima, Makiko Shimizu, and Kentaro Handa
- Subjects
Absorption rate ,Chemical descriptors ,Physiologically based pharmacokinetic modelling ,Tissue concentrations ,Correlation coefficient ,Pharmacokinetics ,Chemistry ,In silico ,General Medicine ,Toxicology ,Biological system ,Systemic circulation - Abstract
Updated algorithms for predicting the volumes of systemic circulation (V1), along with absorption rate constants and hepatic intrinsic clearances, as input parameters for physiologically based pharmacokinetic (PBPK) models were established to improve the accuracy of estimated plasma and tissue concentrations of 323 chemicals after virtual oral administrations in rats. Using ridge regression with an enlarged set of chemical descriptors (up to 99), the estimated input V1 values resulted in an improved correlation coefficient (from 246 compounds) with the traditionally determined values. The PBPK model input parameters for rats of diverse compounds can be precisely estimated by increasing the number of descriptors.
- Published
- 2021
40. Loss of Working Life Years Due to Mortality, Sickness Absence, or Ill-health Retirement: A Comprehensive Approach to Estimating Disease Burden in the Workplace
- Author
-
Seitaro Dohi, Masafumi Eguchi, Naoko Sasaki, Chihiro Nishiura, Tetsuya Mizoue, Akihiko Uehara, Takayuki Ogasawara, Tohru Nakagawa, Makiko Shimizu, Teppei Imai, Isamu Kabe, Maki Konishi, Toru Honda, Kenya Yamamoto, Akiko Nishihara, Shuhei Nomura, Makoto Yamamoto, Hiroko Okazaki, Toshiaki Miyamoto, Yosuke Inoue, Shuichiro Yamamoto, Ai Hori, and Takeshi Kochi
- Subjects
Adult ,Male ,medicine.medical_specialty ,Medicine (General) ,Epidemiology ,sick leave ,030209 endocrinology & metabolism ,Occupational safety and health ,Young Adult ,03 medical and health sciences ,Life Expectancy ,0302 clinical medicine ,R5-920 ,Japan ,death ,Humans ,Medicine ,Prospective Studies ,030212 general & internal medicine ,Mortality ,Prospective cohort study ,Disease burden ,Occupation ,Working life ,Sickness absence ,business.industry ,General Medicine ,Middle Aged ,Occupational Diseases ,workplace ,retirement ,Sick leave ,occupational health ,Female ,Original Article ,Ill health ,business ,Demography - Abstract
Background While much effort has focused on quantifying disease burden in occupational health, no study has simultaneously assessed disease burden in terms of mortality and morbidity. We aimed to propose a new comprehensive method of quantifying the disease burden in the workplace. Methods The data were obtained from the Japan Epidemiology Collaboration on Occupational Health (J-ECOH) Study, a large-scale prospective study of approximately 80,000 workers. We defined disease burden in the workplace as the number of working years lost among the working population during a 6-year period (April 2012 to March 2018). We calculated the disease burden according to consequences of health problems (ie, mortality, sickness absence [SA], and ill-health retirement) and disease category. We also calculated the age-group- (20-39 and 40-59 years old) and sex-specific disease burden. Results The largest contributors to disease burden in the workplace were mental and behavioural disorders (47.0 person-years lost per 10,000 person-years of working years; ie, per myriad [proportion]), followed by neoplasms (10.8 per myriad) and diseases of the circulatory system (7.1 per myriad). While mental and behavioural disorders made a greater contribution to SA and ill-health retirement compared to mortality, the latter two disorders were the largest contributors to the disease burden in the workplace due to mortality. The number of working years lost was greater among younger versus older female participants, whereas the opposite trend was observed in males. Conclusions Our approach is in contrast to those in previous studies that focused exclusively on mortality or morbidity.
- Published
- 2021
41. Roles of human cytochrome P450 3A4/5 in dexamethasone 6β-hydroxylation mediated by liver microsomes and humanized liver in chimeric mice metabolically suppressed with azamulin
- Author
-
Shotaro Uehara, Makiko Shimizu, Hiroshi Suemizu, and Hiroshi Yamazaki
- Subjects
Pharmacology ,Pharmaceutical Science ,Pharmacology (medical) - Published
- 2023
42. A family study of compound variants of flavin-containing monooxygenase 3 (FMO3) in Japanese subjects found by urinary phenotyping for trimethylaminuria
- Author
-
Makiko Shimizu, Akane Yamamoto, Miaki Makiguchi, Erika Shimamura, Yuka Yokota, Mizuki Harano, and Hiroshi Yamazaki
- Subjects
Pharmacology ,Pharmaceutical Science ,Pharmacology (medical) - Published
- 2023
43. Variants of Flavin-containing Monooxygenase 3 (FMO3) Found in Subjects in an Updated Database of Genome Resources
- Author
-
Miaki Makiguchi, Makiko Shimizu, Yuka Yokota, Erika Shimamura, Eiji Hishinuma, Sakae Saito, Masahiro Hiratsuka, and Hiroshi Yamazaki
- Subjects
Pharmacology ,Pharmaceutical Science - Published
- 2023
44. High hepatic and plasma exposures of atorvastatin in subjects harboring impaired cytochrome P450 3A4∗16 modeled after virtual administrations and possibly associated with statin intolerance found in the Japanese adverse drug event report database
- Author
-
Koichiro Adachi, Katsuhiro Ohyama, Yoichi Tanaka, Tasuku Sato, Norie Murayama, Makiko Shimizu, Yoshiro Saito, and Hiroshi Yamazaki
- Subjects
Pharmacology ,Pharmaceutical Science ,Pharmacology (medical) - Published
- 2023
45. TOPOLOGY OPTIMIZATION OF STRUCTURES USING CA-IESO METHOD
- Author
-
Daiji FUJII, Masaki YAMASHITA, and Makiko SHIMIZU
- Subjects
Architecture ,Building and Construction - Published
- 2021
46. Visit-to-visit variability of blood pressure and cardiovascular events among the working-age population in Japan: findings from the Japan Epidemiology Collaboration on Occupational Health Study
- Author
-
Yosuke Inoue, Naoko Sasaki, Ai Hori, Kentaro Tomita, Keisuke Kuwahara, Makiko Shimizu, Akihiko Uehara, Shuichiro Yamamoto, Hiroko Okazaki, Tohru Nakagawa, Masafumi Eguchi, Takayuki Ogasawara, Isamu Kabe, Satsue Nagahama, Takeshi Kochi, Seitaro Dohi, Toshiaki Miyamoto, Huan Hu, Toru Honda, Makoto Yamamoto, and Tetsuya Mizoue
- Subjects
medicine.medical_specialty ,Physiology ,Proportional hazards model ,business.industry ,Hazard ratio ,030204 cardiovascular system & hematology ,Confidence interval ,Occupational safety and health ,03 medical and health sciences ,0302 clinical medicine ,Mean blood pressure ,Blood pressure ,Epidemiology ,Internal Medicine ,Medicine ,Working population ,030212 general & internal medicine ,Cardiology and Cardiovascular Medicine ,business ,Demography - Abstract
Increased visit-to-visit variability (VVV) of blood pressure, which is calculated based on several readings, has been suggested to be a significant predictor of cardiovascular events and mortality, independent of mean blood pressure. This study examined associations between the VVV of systolic blood pressure (SBP) measured annually and cardiovascular disease (CVD) events among 72,617 Japanese subjects. Data were obtained from the Japan Epidemiology Collaboration on Occupational Health Study, which is an ongoing epidemiological survey of workers in Japan. VVV was calculated as the coefficient of variation of SBP readings from 2008 to 2011; information on fatal and nonfatal CVD events was collected from registries of specific outcomes between April 2012 and March 2019. A Cox proportional hazards model was applied to investigate associations after adjusting for mean SBP between 2008 and 2011 and covariates. During the 7-year follow-up period, there were 63 CVD fatalities and 314 CVD events (combining fatal and nonfatal events). The results showed that a one-standard deviation increase in VVV was associated with a significant increase in the risk of CVD mortality (hazard ratio [HR] = 1.42; 95% confidence interval [CI] = 1.32–1.54); those in the highest tertile had a 3.20 times (95% CI = 1.26–8.17) higher risk of CVD mortality than those in the lowest tertile. We found less pronounced associations regarding CVD events (HR = 1.08, 95% CI = 1.02–1.15). In conclusion, VVV was significantly associated with CVD mortality in our Japanese working population.
- Published
- 2021
47. Hepatotoxicological potential of P-toluic acid in humanised-liver mice investigated using simplified physiologically based pharmacokinetic models
- Author
-
Shotaro Uehara, Tomonori Miura, Yusuke Kamiya, Makiko Shimizu, Hiroshi Suemizu, Hiroshi Yamazaki, and Norie Murayama
- Subjects
Pharmacology ,Chromatography ,Health, Toxicology and Mutagenesis ,Metabolite ,Organic solvent ,Xylene ,General Medicine ,Toxicology ,030226 pharmacology & pharmacy ,Biochemistry ,p-Toluic acid ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Pharmacokinetics ,chemistry ,030220 oncology & carcinogenesis ,Glycine - Abstract
p-Toluic acid, a metabolite of organic solvent xylene, has a high reported no‐observed‐effect level (NOEL, 1000 mg/kg) in rats, possibly because of direct glycine conjugation to methylhippuric acid...
- Published
- 2021
48. Metabolic Syndrome and the Increased Risk of Medically Certified Long-term Sickness Absence: A Prospective Analysis Among Japanese Workers.
- Author
-
Hoang, Dong V., Akter, Shamima, Yosuke Inoue, Keisuke Kuwahara, Ami Fukunaga, Islam, Zobida, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Hiroko Okazaki, Toshiaki Miyamoto, Takayuki Ogasawara, Naoko Sasaki, Akihiko Uehara, Makoto Yamamoto, Takeshi Kochi, Masafumi Eguchi, Taiki Shirasaka, Makiko Shimizu, and Satsue Nagahama
- Published
- 2023
- Full Text
- View/download PDF
49. Trivariate Linear Regression and Machine Learning Prediction of Possible Roles of Efflux Transporters in Estimated Intestinal Permeability Values of 301 Disparate Chemicals
- Author
-
Makiko Shimizu, Riku Hayasaka, Yusuke Kamiya, and Hiroshi Yamazaki
- Subjects
Pharmacology ,Machine Learning ,Linear Models ,Pharmaceutical Science ,Humans ,Membrane Transport Proteins ,Biological Transport ,General Medicine ,Caco-2 Cells ,Permeability - Abstract
A system for predicting apparent bidirectional permeability (P
- Published
- 2022
50. Probe drug T-1032 N-oxygenation mediated by cytochrome P450 3A5 in human hepatocytes in vitro and in humanized-liver mice in vivo
- Author
-
Shotaro Uehara, Makiko Shimizu, Karen Plé, Sylvain Routier, Nao Yoneda, Yuichiro Higuchi, Hiroshi Suemizu, Hiroshi Yamazaki, Central Institute for Experimental Animals [Kawasaki, Japan] (CIEA), Showa Pharmaceutical University [Tokyo, Japan], Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research 20K06463 and 20K07164, the Centre Val de Loire Region, France (APR-IR FLUPO), the RTR Motivhealth (2019–00131403, the Feder Techsab (FEDER-FSE 2014-2020-EX011313), ANR-11-LABX-0029,SYNORG,Synthèse Organique : des molécules au vivant(2011), ANR-11-LABX-0018,IRON,Radiopharmaceutiques Innovants en Oncologie et Neurologie(2011), PLE, Karen, Synthèse Organique : des molécules au vivant - - SYNORG2011 - ANR-11-LABX-0029 - LABX - VALID, and Radiopharmaceutiques Innovants en Oncologie et Neurologie - - IRON2011 - ANR-11-LABX-0018 - LABX - VALID
- Subjects
Pharmacology ,CYP3A5.1 ,Pyridines ,[SDV]Life Sciences [q-bio] ,Pharmaceutical Science ,Oxides ,Isoquinolines ,CYP3A5∗1 ,N-oxide ,[SDV] Life Sciences [q-bio] ,Mice ,Liver ,Pharmaceutical Preparations ,Hepatocytes ,T-5 ,Animals ,Cytochrome P-450 CYP3A ,Humans ,Pharmacology (medical) - Abstract
International audience; Polymorphic cytochrome P450 3A5 (CYP3A5) expression contributes to individual differences in the pharmacokinetics of probe drugs. The identification of suitable in vivo CYP3A5 probes would benefit drug metabolism and drug interaction studies using chimeric mice with humanized liver. In this study, we investigated the pharmacokinetic profiles of T-1032, which is known as an in vitro CYP3A5 probe substrate, using humanized-liver mice. Substantial N-oxygenation of T-1032 was observed in hepatocytes from humans and from humanized-liver mice. Hepatocytes from the human donor genotyped as CYP3A5∗3/∗3 (poor expressers) showed significantly lower T-1032 N-oxidation rates than those from donors harboring CYP3A5∗1. After a single oral dose of T-1032 (1.0 mg/kg) in humanized-liver mice, the plasma levels of T-1032 N-oxide were higher in five mice with CYP3A5∗1/∗7 hepatocytes than in four mice with CYP3A5∗3/∗3 hepatocytes. The maximum concentrations of T-1032 N-oxide after oral administration of T-1032 in humanized-liver mice with CYP3A5∗1/∗7 hepatocytes were twice (a significant difference) those from humanized-liver mice with CYP3A5∗3/∗3 hepatocytes. These results suggest that polymorphic CYP3A5-dependent T-1032 N-oxidation was observed in humanized liver mice in vitro and in vivo. However, the contribution of CYP3A5 genotypes may have little or only limited effects on the overall pharmacokinetic profiles of T-1032 in vivo
- Published
- 2022
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.