1. Structural and dynamical properties of reconstituted myelin sheaths in the presence of myelin proteins MBP and P2 studied by neutron scattering
- Author
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Bruno Demé, Ewout Kemner, Yuri Gerelli, Wiebke Knoll, Francesca Natali, Petri Kursula, Jacques Ollivier, Judith Peters, Mark T. F. Telling, Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Department of Biochemistry, University of Oulu, Department of Physics - University of Parma, University of Parma = Università degli studi di Parma [Parme, Italie], Institut Laue-Langevin (ILL), ILL, School of Chemical and Physical Sciences (MacDiarmid Institute for Advanced Materials and Nanotechnology), Victoria University of Wellington, Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Università degli studi di Parma = University of Parma (UNIPR)
- Subjects
Nervous system ,MESH: Myelin Basic Protein ,Proteolipid protein 1 ,MESH: Myelin Sheath ,Lipid Bilayers ,Static Electricity ,Neutron scattering ,MESH: Recombinant Proteins ,Myelin ,MESH: Protein Structure, Tertiary ,Protein structure ,Scattering, Small Angle ,medicine ,Humans ,Myelin Sheath ,MESH: Scattering, Small Angle ,MESH: Static Electricity ,MESH: Humans ,biology ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Chemistry ,MESH: Lipid Bilayers ,Membrane structure ,Temperature ,Myelin Basic Protein ,General Chemistry ,Condensed Matter Physics ,Recombinant Proteins ,MESH: Temperature ,Myelin basic protein ,Protein Structure, Tertiary ,MESH: Neutron Diffraction ,Crystallography ,Neutron Diffraction ,Membrane ,medicine.anatomical_structure ,nervous system ,Biophysics ,biology.protein - Abstract
International audience; The myelin sheath is a tightly packed, multilayered membrane structure wrapped around selected nerve axons in the central and the peripheral nervous system. Because of its electrical insulation of the axons, which allows fast, saltatory nerve impulse conduction, myelin is crucial for the proper functioning of the vertebrate nervous system. A subset of myelin-specific proteins is well-defined, but their influence on membrane dynamics, i.e. myelin stability, has not yet been explored in detail. We investigated the structure and the dynamics of reconstituted myelin membranes on a pico- to nanosecond timescale, influenced by myelin basic protein (MBP) and myelin protein 2 (P2), using neutron diffraction and quasi-elastic neutron scattering. A model for the scattering function describing molecular lipid motions is suggested. Although dynamical properties are not affected significantly by MBP and P2 proteins, they act in a highly synergistic manner influencing the membrane structure.
- Published
- 2014