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Melatonin promotes myelination by decreasing white matter inflammation after neonatal stroke

Authors :
Olivier Baud
Valérie Biran
Christiane Charriaut-Marlangue
Sébastien Fau
Sonia Villapol
Sylvain Renolleau
Physiopathologie, conséquences fonctionnelles et neuroprotection des atteintes du cerveau en développement
Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM)
ANTE-INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM)
Mairie de Paris PremUP fundation
Dojat, Michel
Source :
Pediatric Research, Pediatric Research, Nature Publishing Group, 2011, 69 (1), pp.51-5. ⟨10.1203/PDR.0b013e3181fcb40b⟩
Publication Year :
2011
Publisher :
HAL CCSD, 2011.

Abstract

International audience; Melatonin demonstrates neuroprotective properties in adult models of cerebral ischemia, acting as a potent antioxidant and anti-inflammatory agent. We investigated the effect of melatonin in a 7-d-old rat model of ischemia-reperfusion, leading to both cortical infarct and injury in the underlying white matter observed using MRI and immunohistochemistry. Melatonin was given i.p. as either a single dose before ischemia or a double-dose regimen, combining one before ischemia and one 24 h after reperfusion. At 48 h after injury, neither a significant reduction in cortical infarct volume nor a variation in the number of TUNEL- and nitrotyrosine-positive cells within the ipsilateral lesion was observed in melatonin-treated animals compared with controls. However, a decrease in the density of tomato lectin-positive cells after melatonin treatment was found in the white matter underlying cortical lesion. Furthermore, we showed a marked increase in the myelin basic protein-immunoreactivity in the cingulum and in the density of mature oligodendrocytes (APC-immunoreactive) in both the ipsilateral cingulum and external capsule. These results suggest that melatonin is not able to reduce cortical infarct volume in a neonatal stroke model but strongly reduces inflammation and promotes subsequent myelination in the white matter.

Details

Language :
English
ISSN :
00313998 and 15300447
Database :
OpenAIRE
Journal :
Pediatric Research, Pediatric Research, Nature Publishing Group, 2011, 69 (1), pp.51-5. ⟨10.1203/PDR.0b013e3181fcb40b⟩
Accession number :
edsair.doi.dedup.....aa2cf779188a4709e6a576f2004bc363