1. The coordinated replication of Vibrio cholerae’s two chromosomes required the acquisition of a unique domain by the RctB initiator
- Author
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Fournes, Florian, Niault, Theophile, Czarnecki, Jakub, Tissier-Visconti, Alvise, Mazel, Didier, Val, Marie-Eve, Plasticité du Génome Bactérien - Bacterial Genome Plasticity (PGB), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Sorbonne université - Faculté des Sciences et Ingénierie (SU FSI), Sorbonne Université (SU), Institute of Microbiology [Warsaw], Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW), Institut Pasteur, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique [CNRS-UMR 3525], French National Research Agency, Jeunes Chercheurs [ANR-19-CE12-0001], Laboratoires d’Excellence [ANR-10-LABX-62-IBEID], F.F. was supported by ANR-10-LABX-62-IBEID and ANR-19-CE12-0001, T.N. was supported by the Ministère de l’Enseignement Supérieur et de la Recherche, J.C. was supported by Institut Pasteur (Cantarini foundation) [ANR-19-CE12-0001, ANR-10-LABX-62-IBEID]. Funding for open access charge: French National Research Agency [ANR-19-CE12-0001]., ANR-19-CE12-0001,RepliChroms,Contrôle de la Réplication chez les Bactéries à Multiple Chromosomes(2019), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Signal Transduction ,Models, Molecular ,Protein Conformation, alpha-Helical ,MESH: Sequence Homology, Amino Acid ,AcademicSubjects/SCI00010 ,MESH: DNA Replication ,MESH: Amino Acid Sequence ,MESH: Base Sequence ,Genome Integrity, Repair and Replication ,MESH: Recombinant Proteins ,MESH: Genetic Vectors ,MESH: Replication Origin ,Cloning, Molecular ,MESH: Bacterial Proteins ,Vibrio cholerae ,MESH: Gene Expression Regulation, Bacterial ,MESH: Escherichia coli ,Chromosomes, Bacterial ,Recombinant Proteins ,MESH: Protein Conformation, beta-Strand ,MESH: Models, Molecular ,Protein Binding ,Signal Transduction ,DNA Replication ,DNA, Bacterial ,MESH: Chromosomes, Bacterial ,Genetic Vectors ,MESH: Sequence Alignment ,MESH: Binding, Competitive ,Replication Origin ,Binding, Competitive ,Bacterial Proteins ,Escherichia coli ,MESH: Protein Binding ,MESH: Cloning, Molecular ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Protein Interaction Domains and Motifs ,Amino Acid Sequence ,MESH: Protein Conformation, alpha-Helical ,MESH: Protein Interaction Domains and Motifs ,Binding Sites ,Base Sequence ,Sequence Homology, Amino Acid ,fungi ,Gene Expression Regulation, Bacterial ,MESH: DNA, Bacterial ,MESH: Binding Sites ,nervous system ,Protein Conformation, beta-Strand ,Sequence Alignment ,MESH: Vibrio cholerae - Abstract
International audience; Vibrio cholerae, the pathogenic bacterium that causes cholera, has two chromosomes (Chr1, Chr2) that replicate in a well-orchestrated sequence. Chr2 initiation is triggered only after the replication of the crtS site on Chr1. The initiator of Chr2 replication, RctB, displays activities corresponding with its different binding sites: initiator at the iteron sites, repressor at the 39m sites, and trigger at the crtS site. The mechanism by which RctB relays the signal to initiate Chr2 replication from crtS is not well-understood. In this study, we provide new insights into how Chr2 replication initiation is regulated by crtS via RctB. We show that crtS (on Chr1) acts as an anti-inhibitory site by preventing 39m sites (on Chr2) from repressing initiation. The competition between these two sites for RctB binding is explained by the fact that RctB interacts with crtS and 39m via the same DNA-binding surface. We further show that the extreme C-terminal tail of RctB, essential for RctB self-interaction, is crucial for the control exerted by crtS. This subregion of RctB is conserved in all Vibrio, but absent in other Rep-like initiators. Hence, the coordinated replication of both chromosomes likely results from the acquisition of this unique domain by RctB.
- Published
- 2021