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2 results on '"M, Somenzini"'

1. Multicenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing

Author

J, Bienvenu, M S, Graziani, F, Arpin, H, Bernon, C, Blessum, C, Marchetti, G, Righetti, M, Somenzini, G, Verga, and F, Aguzzi

Subjects
Quality Control, Nephrotic Syndrome, Antibodies, Monoclonal, Electrophoresis, Capillary, Fibrinogen, Reproducibility of Results, Bilirubin, Blood Proteins, Electrophoresis, Cellulose Acetate, Sensitivity and Specificity, Hemoglobins, Nephelometry and Turbidimetry, Reference Values, Humans, Serum Globulins, gamma-Globulins, Laboratories, Immunoelectrophoresis, Serum Albumin, Triglycerides
Abstract

Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

Published
1998

2. Multicenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing.

Author

Bienvenu J, Graziani MS, Arpin F, Bernon H, Blessum C, Marchetti C, Righetti G, Somenzini M, Verga G, and Aguzzi F

Subjects
Bilirubin blood, Electrophoresis, Capillary methods, Electrophoresis, Cellulose Acetate methods, Fibrinogen, Hemoglobins, Humans, Immunoelectrophoresis methods, Laboratories standards, Nephelometry and Turbidimetry methods, Nephrotic Syndrome blood, Quality Control, Reference Values, Reproducibility of Results, Sensitivity and Specificity, Serum Albumin isolation & purification, Serum Globulins isolation & purification, Triglycerides blood, gamma-Globulins isolation & purification, Antibodies, Monoclonal, Blood Proteins isolation & purification, Electrophoresis, Capillary instrumentation
Abstract

Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was < 0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations > 10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

Published
1998

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