1. Duck enteritis virus UL7 is a late gene and the UL7-encoded protein co-localizes with pUL51.
- Author
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Jie Huang, Mingshu Wang, Anchun Cheng, Bin Tian, Xuming Ou, Ying Wu, Qiao Yang, Di Sun, Shaqiu Zhang, Sai Mao, Xinxin Zhao, Juan Huang, Qun Gao, Dekang Zhu, Renyong Jia, Shun Chen, and Mafeng Liu
- Subjects
ENTERITIS ,IMMUNOFLUORESCENCE ,DUCKS ,FIBROBLASTS ,GENE transfection - Abstract
Background: Duck enteritis virus (DEV) belongs to Alphaherpesvirinae; little is known about the DEV UL7 gene and its encoded protein. This study examined the molecular characteristics of DEV pUL7 in vitro and determined whether DEV pUL7 co-localizes with pUL51. Results: The results showed that UL7 can be regarded as a late gene. Moreover, immunofluorescence assay revealed that pUL7 was located around the perinuclear cytoplasmic region and co-localized with pUL51 in the cytoplasm and nucleus after transfection into duck embryo fibroblast cells (DEFs). Conclusion: In conclusion, we identified the molecular characteristics of the DEV UL7 gene, which is a late gene, and the co-localization of its encoded protein with pUL51 in transfected DEFs, enriching our understanding of pUL7 and future research directions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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