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Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16
- Source :
- BMC Veterinary Research, Vol 16, Iss 1, Pp 1-11 (2020), BMC Veterinary Research
- Publication Year :
- 2020
- Publisher :
- BMC, 2020.
-
Abstract
- Background pUL21 is a conserved protein of Alphaherpesvirinae that performs multiple important functions. The C-terminus of pUL21 in other members of this subfamily has RNA-binding ability; this domain contributes to pseudorabies virus (PRV) retrograde axonal transport in vitro and in vivo and participates in newly replicated viral DNA packaging and intracellular virus transport. However, knowledge regarding duck enteritis virus (DEV) pUL21 is limited. Results We verified that DEV UL21 is a γ2 gene that encodes a structural protein. Moreover, we observed that pUL21 localized to the nucleus and cytoplasm. DEV pUL21 interacted with pUL16 and formed a complex in transfected human embryonic kidney (HEK) 293 T cells and DEV-infected duck embryo fibroblasts (DEFs). These results were further confirmed by CO-IP assays. Conclusions The DEV UL21 gene is a late gene, and pUL21 localizes to the nucleus and cytoplasm. DEV UL21 is a virion component. In addition, pUL21 can interact with pUL16. These findings provide insight into the characteristics of UL21 and the interaction between pUL21 and its binding partner pUL16. Our study enhances the understanding of DEV pUL21.
- Subjects :
- Gene Expression Regulation, Viral
animal structures
Interaction
viruses
Pseudorabies
Virus Replication
Virus
03 medical and health sciences
Viral Proteins
Alphaherpesvirinae
Animals
Humans
Gene
Cells, Cultured
Poultry Diseases
030304 developmental biology
0303 health sciences
lcsh:Veterinary medicine
General Veterinary
biology
030306 microbiology
urogenital system
HEK 293 cells
UL21
Virion
General Medicine
Transfection
Herpesviridae Infections
Fibroblasts
biology.organism_classification
Cell biology
Late gene
Mardivirus
Duck enteritis virus
Ducks
HEK293 Cells
Cytoplasm
embryonic structures
Axoplasmic transport
lcsh:SF600-1100
UL16
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17466148
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....cad1af017fccf335d31727f54cdf35b4