1. Characterization, phytochemical profiling, antioxidant, and cytotoxicity of underutilized medicinal plants and composite flour.
- Author
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Singh N, Tyagi N, Singh M, Kushwaha HR, Sharma RK, Shree P, Mittal A, Chopra R, and Garg M
- Subjects
- Humans, A549 Cells, Phenols chemistry, Antioxidants chemistry, Antioxidants pharmacology, Plant Extracts chemistry, Plant Extracts pharmacology, Phytochemicals chemistry, Phytochemicals pharmacology, Plants, Medicinal chemistry, Flour analysis, Cell Survival drug effects
- Abstract
In this investigation, three medicinal plant powders and a composite flour developed from them were analyzed. FESEM/EDS illustrated irregularly shaped particles in the plant powders except for Withania, which had round to oval shape particles. XRD analysis displayed a semi-crystalline nature of powders, except for Asparagus, which showed amorphous behavior. Both methanol and ethanol plant extracts exhibited significantly higher antioxidants, total phenols, and cell viability. Amongst, optimized composite flour (OCF) methanolic extract demonstrated the highest total phenolic content (69.2 ± 0.11 μg GAE/ml), potent cell viability against A549 cells (3.35 ± 0.15% at 50 μg/ml), and strong free-radical scavenging activity (48.89 ± 0.67 at 200 μg/ml). GCMS and FTIR analyses of the methanolic extracts demonstrated the presence of essential phytoconstituents and functional groups. In silico studies of the phytocomponents, ethyl isoallocholate, 3-Deoxy-d-mannoic lactone, and 4,5-Diamino-2-hydroxypyrimidine suggested good binding affinity against BAX, P53, and EGFR proteins with no toxicity and a good drug score., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Meenakshi Garg has patent #A composition of optimized composite flour manufactured using medicinal plants or application no. 202311014262 pending to Office of Controller General of Patents, Designs, & Trademarks (Indian Patent Office). If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)
- Published
- 2024
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