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Presence of unique glyoxalase III proteins in plants indicates the existence of shorter route for methylglyoxal detoxification.
- Source :
-
Scientific reports [Sci Rep] 2016 Jan 06; Vol. 6, pp. 18358. Date of Electronic Publication: 2016 Jan 06. - Publication Year :
- 2016
-
Abstract
- Glyoxalase pathway, comprising glyoxalase I (GLY I) and glyoxalase II (GLY II) enzymes, is the major pathway for detoxification of methylglyoxal (MG) into D-lactate involving reduced glutathione (GSH). However, in bacteria, glyoxalase III (GLY III) with DJ-1/PfpI domain(s) can do the same conversion in a single step without GSH. Our investigations for the presence of DJ-1/PfpI domain containing proteins in plants have indicated the existence of GLY III-like proteins in monocots, dicots, lycopods, gymnosperm and bryophytes. A deeper in silico analysis of rice genome identified twelve DJ-1 proteins encoded by six genes. Detailed analysis has been carried out including their chromosomal distribution, genomic architecture and localization. Transcript profiling under multiple stress conditions indicated strong induction of OsDJ-1 in response to exogenous MG. A member of OsDJ-1 family, OsDJ-1C, showed high constitutive expression at all developmental stages and tissues of rice. MG depletion study complemented by simultaneous formation of D-lactate proved OsDJ-1C to be a GLY III enzyme that converts MG directly into D-lactate in a GSH-independent manner. Site directed mutagenesis of Cys-119 to Alanine significantly reduces its GLY III activity indicating towards the existence of functional GLY III enzyme in rice-a shorter route for MG detoxification.
- Subjects :
- Aldehyde Oxidoreductases chemistry
Amino Acid Sequence
Catalytic Domain
Chromosome Mapping
Gene Expression Profiling
Gene Expression Regulation, Plant
Genome, Plant
Metabolic Networks and Pathways
Mutation
Organ Specificity genetics
Oryza classification
Oryza genetics
Oryza metabolism
Phylogeny
Plant Proteins chemistry
Plant Proteins metabolism
Plants classification
Plants genetics
Promoter Regions, Genetic
Protein Interaction Domains and Motifs
Response Elements
Stress, Physiological
Aldehyde Oxidoreductases metabolism
Inactivation, Metabolic
Plants metabolism
Pyruvaldehyde metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26732528
- Full Text :
- https://doi.org/10.1038/srep18358