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ZnO-rGO nanocomposite based bioelectrode for sensitive and ultrafast detection of dopamine in human serum.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2020 Oct 01; Vol. 165, pp. 112347. Date of Electronic Publication: 2020 Jun 05. - Publication Year :
- 2020
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Abstract
- We present a tyrosinase-conjugated zinc oxide-reduced graphene oxide (Tyr/ZnO-rGO) nanocomposite system as a biosensing test-bed for rapid and sensitive detection of dopamine (DA). The bioelectrodes (Tyr/ZnO-rGO/ITO) were designed by covalently immobilizing tyrosinase enzyme on spin-coated films of ZnO-rGO nanocomposite prepared via self-assembly approach. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed fast electron transfer kinetics of ZnO-rGO/ITO electrode. The response studies of the Tyr/ZnO-rGO/ITO bioelectrode revealed ultrafast (0.34 ± 0.09 s) detection of DA in a wide linear dynamic range of 0.1-1500 pM. The significant performance of the biosensor in terms of low detection limit (8.75 ± 0.64 pM) and high sensitivity (39.56 ± 0.41 μA nM <superscript>-1</superscript> ) values is attributed to the fast and unhindered electron transfer mechanism of ZnO-rGO matrix having low electrochemical band gap. The nanoplatform exhibited high selectivity toward DA in human sera, and remained stable up to 3 months at 4 °C, representing its suitability for clinical applications.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Dopamine
Electrodes
Humans
Biosensing Techniques
Graphite
Nanocomposites
Zinc Oxide
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 165
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 32729488
- Full Text :
- https://doi.org/10.1016/j.bios.2020.112347