1. Expression of Podocalyxin Potentially Decorated With Low-sulfated Keratan Sulfate in Human Testicular Embryonal Carcinoma.
- Author
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Muramoto A, Hoshino H, Inamura S, Murahashi M, Akama TO, Terada N, and Kobayashi M
- Subjects
- Humans, Male, Immunohistochemistry, Cell Line, Tumor, Neoplasms, Germ Cell and Embryonal metabolism, Neoplasms, Germ Cell and Embryonal pathology, Neoplasms, Germ Cell and Embryonal diagnosis, Testicular Neoplasms metabolism, Testicular Neoplasms pathology, Testicular Neoplasms diagnosis, Sialoglycoproteins analysis, Sialoglycoproteins metabolism, Carcinoma, Embryonal pathology, Carcinoma, Embryonal metabolism, Keratan Sulfate metabolism, Keratan Sulfate analysis
- Abstract
SummaryWe previously demonstrated that among various histological types of human testicular germinal cell tumors (GCTs), embryonal carcinoma (EC) preferentially expresses low-sulfated keratan sulfate (KS) consisting of repeating N -acetyllactosamine (LacNAc) disaccharide units composed of galactose and 6- O -sulfated N -acetylglucosamine (GlcNAc), which is recognized by the R-10G antibody. Recently, we generated another anti-low-sulfated KS monoclonal antibody, 294-1B1. Immunohistochemical analysis of testicular GCTs ( n =83) revealed that the low-sulfated KS recognized by 294-1B1 is also preferentially expressed in EC but minimally in other GCT histological types. Moreover, immunolabeling with R-10G and 294-1B1 antibodies was resistant to peptide- N -glycosidase F digestion, and EC was not stained with the MECA-79 antibody, indicating that low-sulfated KS expressed in EC contains mucin-type core 2 O -glycans carrying GlcNAc-6- O -sulfated oligo-LacNAc. Double immunofluorescence staining showed that R-10G and 294-1B1 antibody signals colocalized with those for podocalyxin (PODXL). Furthermore, western blot analysis of recombinant human PODXL•IgG fusion proteins secreted from low-sulfated KS-expressing human embryonic kidney 293T cells revealed that PODXL functions as a core protein for low-sulfated KS. Taken together, these findings strongly suggest that the PODXL glycoform decorated with low-sulfated KS is preferentially expressed in human testicular EC and may therefore serve as a diagnostic marker for this malignancy., Competing Interests: Competing InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
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