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Fibromodulin and Biglycan Modulate Periodontium through TGFβ/BMP Signaling.
- Source :
-
Journal of dental research [J Dent Res] 2014 Aug; Vol. 93 (8), pp. 780-7. Date of Electronic Publication: 2014 Jun 25. - Publication Year :
- 2014
-
Abstract
- A full understanding of the key regulators controlling periodontal development and homeostasis is necessary for the design of improved periodontal regenerative therapies. Small leucine-rich proteoglycans (SLRPs) are extracellular matrix molecules suggested to regulate collagen organization and cell signaling. Mice with double-deficiency of 2 SLRPs, fibromodulin and biglycan (dKO), acquire skeletal abnormalities, but their roles in regulating the periodontium remain undefined and were the focus of our studies. Transmission electron microscopy studies showed abnormal collagen fibrils in the periodontal ligament (PDL) and altered remodeling of alveolar bone in dKO mice. Immunohistochemistry (IHC) revealed increased staining of SLRPs (asporin, lumican, and decorin) and dentin matrix protein-1 (DMP1, a mechanosensory/osteocyte marker), while osteoblast markers, bone sialoprotein and osteopontin, remained unchanged. Disruption of homeostasis was further evidenced by increased expression of receptor-activator of nuclear factor-κB ligand (RANKL) and elevated numbers of osteoclasts, especially noted around the alveolar bone of molars (buccal side) and incisors. Polymerase chain reaction (PCR) array revealed hyperactive transforming growth factors beta/bone morphogenetic protein (TGFβ/BMP) signaling in dKO PDL tissues, which was further confirmed by elevated expression of phosphorylated Smad5 (p-Smad5) by IHC in dKO PDL. These studies highlight the importance of SLRPs in maintaining periodontal homeostasis through regulation of TGFβ/BMP signaling, matrix turnover, and collagen organization.<br /> (© International & American Associations for Dental Research.)
- Subjects :
- Alveolar Process pathology
Alveolar Process physiology
Animals
Bone Remodeling physiology
Chondroitin Sulfate Proteoglycans analysis
Collagen ultrastructure
Decorin analysis
Extracellular Matrix Proteins analysis
Fibromodulin
Homeostasis physiology
Keratan Sulfate analysis
Lumican
Male
Mice
Mice, Knockout
Microscopy, Electron, Transmission
Osteoclasts pathology
Osteopontin analysis
Periodontal Ligament ultrastructure
RANK Ligand analysis
Smad5 Protein analysis
Biglycan physiology
Bone Morphogenetic Proteins physiology
Extracellular Matrix Proteins physiology
Periodontium physiology
Proteoglycans physiology
Signal Transduction physiology
Transforming Growth Factor beta physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1544-0591
- Volume :
- 93
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of dental research
- Publication Type :
- Academic Journal
- Accession number :
- 24966230
- Full Text :
- https://doi.org/10.1177/0022034514541126