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181 results on '"Jean-Olivier Durand"'

1. Rhabdomyosarcoma targeting with tuned porous silicon nanoparticles

Author: Sofia Dominguez‐Gil, Rita Sala, Victoria Judith Morel, Christophe Nguyen, Khaled El Cheikh, Alain Morère, Jean‐Olivier Durand, Jochen Rössler, Michele Bernasconi, Frédérique Cunin, and Magali Gary‐Bobo
Subjects: mannose 6‐phosphate receptor, porous silicon nanoparticles, Rhabdomyosarcoma targeting, two‐photon excitation photodynamic therapy, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract: Abstract We describe porous silicon nanoparticles (pSiNP) chemically functionalized with an analog of mannose 6‐phosphate (AMFA) and a porphyrin derivative to target aggressive pediatric Rhabdomyosarcoma (RMS) tumor cells. Our findings demonstrate that the pSiNP@AMFA@porphyrin nanosystems are efficiently internalized by RMS cells, which overexpress mannose 6‐phosphate receptors, and induce cytotoxicity and phototoxicity when exposed to two‐photon excitation light. These results provide an interesting potential for targeting and treating RMS pediatric tumors.
Published: 2024
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2. Upscale Synthesis of Magnetic Mesoporous Silica Nanoparticles and Application to Metal Ion Separation: Nanosafety Evaluation

Author: Mathilde Ménard, Lamiaa M. A. Ali, Ani Vardanyan, Clarence Charnay, Laurence Raehm, Frédérique Cunin, Aurélie Bessière, Erwan Oliviero, Theodossis A. Theodossiou, Gulaim A. Seisenbaeva, Magali Gary-Bobo, and Jean-Olivier Durand
Subjects: magnetic mesoporous nanoparticles, upscaled synthesis, DTPA, metal ion separation, toxicity, Chemistry, QD1-999
Abstract: The synthesis of core–shell magnetic mesoporous nanoparticles (MMSNs) through a phase transfer process is usually performed at the 100–250 mg scale. At the gram scale, nanoparticles without cores or with multicore systems are observed. Iron oxide core nanoparticles (IO) were synthesized through a thermal decomposition procedure of α-FeO(OH) in oleic acid. A phase transfer from chloroform to water was then performed in order to wrap the IO nanoparticles with a mesoporous silica shell through the sol–gel procedure. MMSNs were then functionalized with DTPA (diethylenetriaminepentacetic acid) and used for the separation of metal ions. Their toxicity was evaluated. The phase transfer procedure was crucial to obtaining MMSNs on a large scale. Three synthesis parameters were rigorously controlled: temperature, time and glassware. The homogeneous dispersion of MMSNs on the gram scale was successfully obtained. After functionalization with DTPA, the MMSN-DTPAs were shown to have a strong affinity for Ni ions. Furthermore, toxicity was evaluated in cells, zebrafish and seahorse cell metabolic assays, and the nanoparticles were found to be nontoxic. We developed a method of preparing MMSNs at the gram scale. After functionalization with DTPA, the nanoparticles were efficient in metal ion removal and separation; furthermore, no toxicity was noticed up to 125 µg mL−1 in zebrafish.
Published: 2023
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3. Allogenic Stem Cells Carried by Porous Silicon Scaffolds for Active Bone Regeneration In Vivo

Author: Matthieu Renaud, Philippe Bousquet, Gerard Macias, Gael Y. Rochefort, Jean-Olivier Durand, Lluis F. Marsal, Frédéric Cuisinier, Frédérique Cunin, and Pierre-Yves Collart-Dutilleul
Subjects: tissue engineering, bone regeneration, mesenchymal stem cells, bone substitute, dental pulp stem cells, porous silicon, Technology, Biology (General), QH301-705.5
Abstract: To date, bone regeneration techniques use many biomaterials for bone grafting with limited efficiencies. For this purpose, tissue engineering combining biomaterials and stem cells is an important avenue of development to improve bone regeneration. Among potentially usable non-toxic and bioresorbable scaffolds, porous silicon (pSi) is an interesting biomaterial for bone engineering. The possibility of modifying its surface can allow a better cellular adhesion as well as a control of its rate of resorption. Moreover, release of silicic acid upon resorption of its nanostructure has been previously proved to enhance stem cell osteodifferentiation by inducing calcium phosphate formation. In the present study, we used a rat tail model to experiment bone tissue engineering with a critical size defect. Two groups with five rats per group of male Wistar rats were used. In each rat, four vertebrae were used for biomaterial implantation. Randomized bone defects were filled with pSi particles alone or pSi particles carrying dental pulp stem cells (DPSC). Regeneration was evaluated in comparison to empty defect and defects filled with xenogenic bone substitute (Bio-Oss®). Fluorescence microscopy and SEM evaluations showed adhesion of DPSCs on pSi particles with cells exhibiting distribution throughout the biomaterial. Histological analyzes revealed the formation of a collagen network when the defects were filled with pSi, unlike the positive control using Bio-Oss®. Overall bone formation was objectivated with µCT analysis and showed a higher bone mineral density with pSi particles combining DPSC. Immunohistochemical assays confirmed the increased expression of bone markers (osteocalcin) when pSi particles carried DPSC. Surprisingly, no grafted cells remained in the regenerated area after one month of healing, even though the grafting of DPSC clearly increased bone regeneration for both bone marker expression and overall bone formation objectivated with µCT. In conclusion, our results show that the association of pSi with DPSCs in vivo leads to greater bone formation, compared to a pSi graft without DPSCs. Our results highlight the paracrine role of grafted stem cells by recruitment and stimulation of endogenous cells.
Published: 2023
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4. Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading

Author: Haneen Omar, Sara Jakimoska, Julia Guillot, Edreese Alsharaeh, Clarence Charnay, Frédérique Cunin, Aurélie Bessière, Jean-Olivier Durand, Laurence Raehm, Laure Lichon, Mélanie Onofre, and Magali Gary-Bobo
Subjects: dendritic mesoporous organosilica nanoparticles, siRNA, FVIII factor, Organic chemistry, QD241-441
Abstract: Dendritic mesoporous organosilica nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecule delivery. We studied the photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON to investigate new ways for DMON to escape from the endosomes-lysosomes and deliver biomolecules into the cytoplasm of cells. We added photosensitizers in the framework of DMON and found that DMON were loaded with siRNA or FVIII factor protein. We made four formulations with four different photosensitizers. The photosensitizers allowed us to perform imaging of DMON in cancer cells, but the presence of the tetrasulfide bond in the framework of DMON quenched the formation of singlet oxygen. Fortunately, one formulation allowed us to efficiently deliver proapoptotic siRNA in MCF-7 cancer cells leading to 31% of cancer cell death, without irradiation. As for FVIII protein, it was loaded in two formulations with drug-loading capacities (DLC) up to 25%. In conclusion, DMON are versatile nanoparticles capable of loading siRNA and delivering it into cancer cells, and also loading FVIII protein with good DLC. Due to the presence of tetrasulfide, it was not possible to perform PDT or PCI.
Published: 2023
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5. Photosensitivity of Different Nanodiamond–PMO Nanoparticles in Two-Photon-Excited Photodynamic Therapy

Author: Nicolas Bondon, Denis Durand, Kamel Hadj-Kaddour, Lamiaa M. A. Ali, Rabah Boukherroub, Nadir Bettache, Magali Gary-Bobo, Laurence Raehm, Jean-Olivier Durand, Christophe Nguyen, and Clarence Charnay
Subjects: nanodiamond, two-photon excitation, mesoporous organosilica, theranostics, cancer cells, Science
Abstract: Background: In addition to their great optical properties, nanodiamonds (NDs) have recently proved useful for two-photon-excited photodynamic therapy (TPE-PDT) applications. Indeed, they are able to produce reactive oxygen species (ROS) directly upon two-photon excitation but not with one-photon excitation; Methods: Fluorescent NDs (FNDs) with a 100 nm diameter and detonation NDs (DNDs) of 30 nm were compared. In order to use the gems for cancer-cell theranostics, they were encapsulated in a bis(triethoxysilyl)ethylene-based (ENE) periodic mesoporous organosilica (PMO) shell, and the surface of the formed nanoparticles (NPs) was modified by the direct grafting of polyethylene glycol (PEG) and amino groups using PEG-hexyltriethoxysilane and aminoundecyltriethoxysilane during the sol–gel process. The NPs’ phototoxicity and interaction with MDA-MB-231 breast cancer cells were evaluated afterwards; Results: Transmission electronic microscopy images showed the formation of core–shell NPs. Infrared spectra and zeta-potential measurements confirmed the grafting of PEG and NH2 groups. The encapsulation of the NDs allowed for the imaging of cancer cells with NDs and for the performance of TPE-PDT of MDA-MB-231 cancer cells with significant mortality. Conclusions: Multifunctional ND@PMO core–shell nanosystems were successfully prepared. The NPs demonstrated high biocompatibility and TPE-PDT efficiency in vitro in the cancer cell model. Such systems hold good potential for two-photon-excited PDT applications.
Published: 2022
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6. Periodic Mesoporous Organosilica Nanoparticles for CO2 Adsorption at Standard Temperature and Pressure

Author: Paul Kirren, Lucile Barka, Saher Rahmani, Nicolas Bondon, Nicolas Donzel, Philippe Trens, Aurélie Bessière, Laurence Raehm, Clarence Charnay, and Jean-Olivier Durand
Subjects: periodic mesoporous organosilica, sol-gel process, CO2 adsorption, carbon capture and storage (CCS) application, Organic chemistry, QD241-441
Abstract: (1) Background: Due to human activities, greenhouse gas (GHG) concentrations in the atmosphere are constantly rising, causing the greenhouse effect. Among GHGs, carbon dioxide (CO2) is responsible for about two-thirds of the total energy imbalance which is the origin of the increase in the Earth’s temperature. (2) Methods: In this field, we describe the development of periodic mesoporous organosilica nanoparticles (PMO NPs) used to capture and store CO2 present in the atmosphere. Several types of PMO NP (bis(triethoxysilyl)ethane (BTEE) as matrix, co-condensed with trialkoxysilylated aminopyridine (py) and trialkoxysilylated bipyridine (Etbipy and iPrbipy)) were synthesized by means of the sol-gel procedure, then characterized with different techniques (DLS, TEM, FTIR, BET). A systematic evaluation of CO2 adsorption was carried out at 298 K and 273 K, at low pressure. (3) Results: The best values of CO2 adsorption were obtained with 6% bipyridine: 1.045 mmol·g−1 at 298 K and 2.26 mmol·g−1 at 273 K. (4) Conclusions: The synthetized BTEE/aminopyridine or bipyridine PMO NPs showed significant results and could be promising for carbon capture and storage (CCS) application.
Published: 2022
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7. Degradable Hollow Organosilica Nanoparticles for Antibacterial Activity

Author: Saher Rahmani, Karim Bouchmella, Jelena Budimir, Laurence Raehm, Mateus Borba Cardoso, Philippe Trens, Jean-Olivier Durand, and Clarence Charnay
Subjects: Chemistry, QD1-999
Published: 2019
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8. Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Author: Binh Thanh Vu, Sophia Allaf Shahin, Jonas Croissant, Yevhen Fatieiev, Kotaro Matsumoto, Tan Le-Hoang Doan, Tammy Yik, Shirleen Simargi, Altagracia Conteras, Laura Ratliff, Chiara Mauriello Jimenez, Laurence Raehm, Niveen Khashab, Jean-Olivier Durand, Carlotta Glackin, and Fuyuhiko Tamanoi
Subjects: Medicine, Science
Abstract: Abstract New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.
Published: 2018
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9. Quaternary Ammonium-Based Ionosilica Hydrogels as Draw Solutes in Forward Osmosis

Author: Alysson Duarte Rodrigues, Matthieu Jacob, Véronique Gauchou, Jean-Olivier Durand, Philippe Trens, and Peter Hesemann
Subjects: ionosilica, hydrogel, forward osmosis, draw solute, Organic chemistry, QD241-441
Abstract: In the last few years, forward osmosis (FO) has attracted increasing interest as a sustainable technique for water desalination and wastewater treatment. However, FO remains as an immature process principally due to the lack of efficient and easily recyclable draw solutes. In this work, we report that ionosilica hydrogels based on quaternary ammonium halide ionosilica are efficient draw solutes in FO. Fluidic ionosilica hydrogels were obtained via hydrolysis-polycondensation reactions of a trisilylated quaternary ammonium precursor in slightly acidic water/ethanol solvent mixtures. The liquid-to-gel transition of the precursor and the kinetics of the formation of hydrogels were monitored by liquid NMR measurements. The formed hydrogels were shown to generate osmotic pressure up to 10.0 atm, indicating the potential of these hydrogels as efficient draw solutes in FO. Our results suggest that iodide anions are the osmotically active species in the system. Regeneration of the hydrogels via ultrafiltration (UF) was successfully achieved, allowing the development of a closed FO-UF process. However, the osmotic performances of the ionosilica hydrogels irreversibly decreased along the successive FO-UF cycles, probably due to anion exchange processes.
Published: 2020
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10. Polythiophenes with Cationic Phosphonium Groups as Vectors for Imaging, siRNA Delivery, and Photodynamic Therapy

Author: Laure Lichon, Clément Kotras, Bauyrzhan Myrzakhmetov, Philippe Arnoux, Morgane Daurat, Christophe Nguyen, Denis Durand, Karim Bouchmella, Lamiaa Mohamed Ahmed Ali, Jean-Olivier Durand, Sébastien Richeter, Céline Frochot, Magali Gary-Bobo, Mathieu Surin, and Sébastien Clément
Subjects: combined therapy, conjugated polyelectrolyte, imaging, photodynamic therapy, polythiophenes, siRNA delivery, Chemistry, QD1-999
Abstract: In this work, we exploit the versatile function of cationic phosphonium-conjugated polythiophenes to develop multifunctional platforms for imaging and combined therapy (siRNA delivery and photodynamic therapy). The photophysical properties (absorption, emission and light-induced generation of singlet oxygen) of these cationic polythiophenes were found to be sensitive to molecular weight. Upon light irradiation, low molecular weight cationic polythiophenes were able to light-sensitize surrounding oxygen into reactive oxygen species (ROS) while the highest were not due to its aggregation in aqueous media. These polymers are also fluorescent, allowing one to visualize their intracellular location through confocal microscopy. The most promising polymers were then used as vectors for siRNA delivery. Due to their cationic and amphipathic features, these polymers were found to effectively self-assemble with siRNA targeting the luciferase gene and deliver it in MDA-MB-231 cancer cells expressing luciferase, leading to 30–50% of the gene-silencing effect. In parallel, the photodynamic therapy (PDT) activity of these cationic polymers was restored after siRNA delivery, demonstrating their potential for combined PDT and gene therapy.
Published: 2020
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11. Periodic Mesoporous Organosilica Nanoparticles with BOC Group, towards HIFU Responsive Agents

Author: Hao Li, Carolina Gascó, Anthony Delalande, Clarence Charnay, Laurence Raehm, Patrick Midoux, Chantal Pichon, Roser Pleixats, and Jean-Olivier Durand
Subjects: hifu, periodic mesoporous organosilica nanoparticles, cancer, co2, Organic chemistry, QD241-441
Abstract: Periodic Mesoporous Organosilica Nanoparticles (PMONPs) are nanoparticles of high interest for nanomedicine applications. These nanoparticles are not composed of silica (SiO2). They belong to hybrid organic−inorganic systems. We considered using these nanoparticles for CO2 release as a contrast agent for High Intensity Focused Ultrasounds (HIFU). Three molecules (P1−P3) possessing two to four triethoxysilyl groups were synthesized through click chemistry. These molecules possess a tert-butoxycarbonyl (BOC) group whose cleavage in water at 90−100 °C releases CO2. Bis(triethoxysilyl)ethylene E was mixed with the molecules Pn (or not for P3) at a proportion of 90/10 to 75/25, and the polymerization triggered by the sol-gel procedure led to PMONPs. PMONPs were characterized by different techniques, and nanorods of 200−300 nm were obtained. These nanorods were porous at a proportion of 90/10, but non-porous at 75/25. Alternatively, molecules P3 alone led to mesoporous nanoparticles of 100 nm diameter. The BOC group was stable, but it was cleaved at pH 1 in boiling water. Molecules possessing a BOC group were successfully used for the preparation of nanoparticles for CO2 release. The BOC group was stable and we did not observe release of CO2 under HIFU at lysosomal pH of 5.5. The pH needed to be adjusted to 1 in boiling water to cleave the BOC group. Nevertheless, the concept is interesting for HIFU theranostic agents.
Published: 2020
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12. Encapsulation of Upconversion Nanoparticles in Periodic Mesoporous Organosilicas

Author: Saher Rahmani, Chiara Mauriello Jimenez, Dina Aggad, Daniel González-Mancebo, Manuel Ocaña, Lamiaa M. A. Ali, Christophe Nguyen, Ana Isabel Becerro Nieto, Nadège Francolon, Erwan Oliveiro, Damien Boyer, Rachid Mahiou, Laurence Raehm, Magali Gary-Bobo, Jean-Olivier Durand, and Clarence Charnay
Subjects: upconversion, periodic mesoporous organosilica nanoparticles, cancer, Organic chemistry, QD241-441
Abstract: (1) Background: Nanomedicine has recently emerged as a promising field, particularly for cancer theranostics. In this context, nanoparticles designed for imaging and therapeutic applications are of interest. We, therefore, studied the encapsulation of upconverting nanoparticles in mesoporous organosilica nanoparticles. Indeed, mesoporous organosilica nanoparticles have been shown to be very efficient for drug delivery, and upconverting nanoparticles are interesting for near-infrared and X-ray computed tomography imaging, depending on the matrix used. (2) Methods: Two different upconverting-based nanoparticles were synthesized with Yb3+-Er3+ as the upconverting system and NaYF4 or BaLuF5 as the matrix. The encapsulation of these nanoparticles was studied through the sol-gel procedure with bis(triethoxysilyl)ethylene and bis(triethoxysilyl)ethane in the presence of CTAB. (3) Results: with bis(triethoxysilyl)ethylene, BaLuF5: Yb3+-Er3+, nanoparticles were not encapsulated, but anchored on the surface of the obtained mesoporous nanorods BaLuF5: Yb3+-Er3+@Ethylene. With bis(triethoxysilyl)ethane, BaLuF5: Yb3+-Er3+ and NaYF4: Yb3+-Er3+nanoparticles were encapsulated in the mesoporous cubic structure leading to BaLuF5: Yb3+-Er3+@Ethane and NaYF4: Yb3+-Er3+@Ethane, respectively. (4) Conclusions: upconversion nanoparticles were located on the surface of mesoporous nanorods obtained by hydrolysis polycondensation of bis(triethoxysilyl)ethylene, whereas encapsulation occurred with bis(triethoxysilyl)ethane. The later nanoparticles NaYF4: Yb3+-Er3+@Ethane or BaLuF5: Yb3+-Er3+@Ethane were promising for applications with cancer cell imaging or X-ray-computed tomography respectively.
Published: 2019
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13. Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

Author: Saher Rahmani, Jelena Budimir, Mylene Sejalon, Morgane Daurat, Dina Aggad, Eric Vivès, Laurence Raehm, Marcel Garcia, Laure Lichon, Magali Gary-Bobo, Jean-Olivier Durand, and Clarence Charnay
Subjects: pepstatin A, mesoporous silica nanoparticles, mesoporous organosilica nanoparticles, cancer, Organic chemistry, QD241-441
Abstract: (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.
Published: 2019
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14. Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications

Author: Wei Liu, Arnaud Chaix, Magali Gary-Bobo, Bernard Angeletti, Armand Masion, Afitz Da Silva, Morgane Daurat, Laure Lichon, Marcel Garcia, Alain Morère, Khaled El Cheikh, Jean-Olivier Durand, Frédérique Cunin, and Mélanie Auffan
Subjects: porous silicon nanoparticle, surface functionalization, PEG, mannose, stealth properties, biodegradation kinetic, biocompatibility, Chemistry, QD1-999
Abstract: A challenge regarding the design of nanocarriers for drug delivery is to prevent their recognition by the immune system. To improve the blood residence time and prevent their capture by organs, nanoparticles can be designed with stealth properties using polymeric coating. In this study, we focused on the influence of surface modification with polyethylene glycol and/or mannose on the stealth behavior of porous silicon nanoparticles (pSiNP, ~200 nm). In vivo biodistribution of pSiNPs formulations were evaluated in mice 5 h after intravenous injection. Results indicated that the distribution in the organs was surface functionalization-dependent. Pristine pSiNPs and PEGylated pSiNPs were distributed mainly in the liver and spleen, while mannose-functionalized pSiNPs escaped capture by the spleen, and had higher blood retention. The most efficient stealth behavior was observed with PEGylated pSiNPs anchored with mannose that were the most excreted in urine at 5 h. The biodegradation kinetics evaluated in vitro were in agreement with these in vivo observations. The biocompatibility of the pristine and functionalized pSiNPs was confirmed in vitro on human cell lines and in vivo by cytotoxic and systemic inflammation investigations, respectively. With their biocompatibility, biodegradability, and stealth properties, the pSiNPs functionalized with mannose and PEG show promising potential for biomedical applications.
Published: 2017
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15. Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading

Author: Gary-Bobo, Haneen Omar, Sara Jakimoska, Julia Guillot, Edreese Alsharaeh, Clarence Charnay, Frédérique Cunin, Aurélie Bessière, Jean-Olivier Durand, Laurence Raehm, Laure Lichon, Mélanie Onofre, and Magali
Subjects: dendritic mesoporous organosilica nanoparticles, siRNA, FVIII factor
Abstract: Dendritic mesoporous organosilica nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecule delivery. We studied the photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON to investigate new ways for DMON to escape from the endosomes-lysosomes and deliver biomolecules into the cytoplasm of cells. We added photosensitizers in the framework of DMON and found that DMON were loaded with siRNA or FVIII factor protein. We made four formulations with four different photosensitizers. The photosensitizers allowed us to perform imaging of DMON in cancer cells, but the presence of the tetrasulfide bond in the framework of DMON quenched the formation of singlet oxygen. Fortunately, one formulation allowed us to efficiently deliver proapoptotic siRNA in MCF-7 cancer cells leading to 31% of cancer cell death, without irradiation. As for FVIII protein, it was loaded in two formulations with drug-loading capacities (DLC) up to 25%. In conclusion, DMON are versatile nanoparticles capable of loading siRNA and delivering it into cancer cells, and also loading FVIII protein with good DLC. Due to the presence of tetrasulfide, it was not possible to perform PDT or PCI.
Published: 2023
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16. Periodic Mesoporous Ionosilica Nanoparticles for BODIPY Delivery and Photochemical Internalization of siRNA

Author: Braham Mezghrani, Lamiaa M. A. Ali, Sara Jakimoska, Frédérique Cunin, Peter Hesemann, Jean‐Olivier Durand, and Nadir Bettache
Subjects: General Chemistry
Published: 2023
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17. Persistent luminescence materials for deep photodynamic therapy

Author: Camille Noûs, Aurélie Bessière, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and Cogitamus Laboratory
Subjects: Materials science, medicine.medical_treatment, QC1-999, Cancer therapy, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Persistent luminescence, medicine, [CHIM]Chemical Sciences, natural sciences, Electrical and Electronic Engineering, Physics, photosensitizers, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, photodynamic therapy, Cancer research, cancer therapy, nanoparticles, 0210 nano-technology, Biotechnology, persistent luminescence
Abstract: Persistent luminescence (PerL) materials continue emitting light long after their excitation has stopped. Prepared in the form of nanoparticles they revealed their full potential as bio-nanoprobes for in vivo small animal imaging in the last 15 years. PerL materials enable to overcome the limitation of weak light penetration in living tissues. As such, they constitute remarkable light mediators to implement photodynamic therapy (PDT) in deep-seated tissues. This article reviews the recent achievements in PerL-mediated PDT in vitro as well as in small animal cancer models in vivo. PerL-mediated PDT is realized through the smart choice of a tandem of a PerL material and a photosensitizer (PS). The physical association of the PerL material and the PS as well as their targeting ability is debated. Implants or mesoporous nanoparticles emerge as particularly valuable cargos that further permit multimodality in imaging or therapy. The diversity of charge-trapping mechanisms in a few PerL materials enables a large versatility in the excitation protocols. Although the PerL agent can be pre-excited by UV light before its introduction into the animal, it also induces effective PDT after simple infrared or visible LED illumination across tissues as well as after a mild X-ray irradiation.
Published: 2021
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18. Periodic Mesoporous Ionosilica Nanoparticles for Dual Cancer Therapy: Two-Photon Excitation siRNA Gene Silencing in Cells and Photodynamic Therapy in Zebrafish Embryos

Author: Braham Mezghrani, Lamiaa M.A. Ali, Nicolas Cubedo, Mireille Rossel, Peter Hesemann, Jean-Olivier Durand, and Nadir Bettache
Subjects: Pharmaceutical Science
Published: 2023
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19. Synthesis of Cyclen‐Functionalized Ethenylene‐Based Periodic Mesoporous Organosilica Nanoparticles and Metal‐Ion Adsorption Studies

Author: Gulaim A. Seisenbaeva, Clarence Charnay, Hao Li, Roser Pleixats, Laurence Raehm, Ani Vardanyan, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Swedish University of Agricultural Sciences (SLU), and Universitat Autònoma de Barcelona (UAB)
Subjects: [CHIM.ORGA]Chemical Sciences/Organic chemistry, Renewable Energy, Sustainability and the Environment, Chemistry, Energy Engineering and Power Technology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Biomaterials, chemistry.chemical_compound, Mesoporous organosilica, Adsorption, Cyclen, Transition metal, Desorption, Polymer chemistry, Materials Chemistry, Click chemistry, Nanorod, Other Chemistry Topics, 0210 nano-technology, Selectivity
Abstract: International audience; The preparation of two cyclens both possessing two triethoxysilyl groups through click chemistry is described. These two cyclens were incorporated into bis(triethoxysilyl)ethenylene‐based periodic mesoporous organosilica nanoparticles (PMO NPs) at different proportions of bis(triethoxysilyl)ethenylene/cyclens (90/10, 75/25). The obtained nanorods were analyzed with different techniques and showed high specific surface areas at low proportion of cyclens. The nanorods containing free amino groups of cyclen were then used for Ni(II) and Co(II) removal from model solutions. The kinetics and isotherms of adsorption of Ni(II) and Co(II) were determined, and the materials showed high uptake of metals (up to 3.9 mmol ⋅ g−1). They demonstrated pronounced selectivity in separation of rare earth elements from late transition metals, e. g. Ni(II) and Co(II) by adsorption and even more so by controlled desorption..
Published: 2020
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20. Periodic Mesoporous Ionosilica Nanoparticles: Versatile Nano-objects for Imaging, Photodynamic Therapy, Drug Delivery and siRNA Gene Silencing

Author: Braham MEZGHRANI, Lamiaa ALI, Peter HESEMANN, Jean-Olivier DURAND, and Nadir BETTACHE
Subjects: Oncology, Biophysics, Pharmacology (medical), Dermatology
Published: 2023
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21. Two-photon responsive fluorophore-porphyrin biodegradable PMO nanoparticles for FRET-enhanced PDT and antibiotic encapsulation

Author: Nicolas BONDON, Clément CHARLOT, Alexandre BARRAS, Christophe NGUYEN, Lamiaa ALI, Denis DURAND, Sabine SZUNERITS, Rabah BOUKHERROUB, Nicolas RICHY, Olivier MONGIN, Nadir BETTACHE, Magali GARY-BOBO, Jean-Olivier DURAND, and Clarence CHARNAY
Subjects: Oncology, Biophysics, Pharmacology (medical), Dermatology
Published: 2023
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22. Porphyrin-Based Mesoporous Organosilica Nanoparticles for Two-Photon Rhabdomyosarcoma Targeting

Author: Morgane DAURAT, Christophe NGUYEN, Sofia Dominguez GIL, Vincent SOL, Vincent CHALEIX, Clarence CHARNAY, Laurence RAEHM, Khaled El CHEIKH, Alain MORERE, Michele BERNASCONI, Andrea TIMPANARO, Marcel GARCIA, Frédérique CUNIN, Jochen ROESSLER, Jean-Olivier DURAND, and Magali GARY-BOBO
Subjects: Oncology, Biophysics, Pharmacology (medical), Dermatology
Published: 2023
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23. Lateral porous silicon interferometric transducer for on-chip flow-through sensing applications

Author: David Bourrier, Douglas Silva de Vasconcellos, Kata Hajdu, Thierry Leichle, Jean-Olivier Durand, Véronique Bardinal, Yingning He, Frédérique Cunin, Équipe Microsystèmes électromécaniques (LAAS-MEMS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Équipe MICrosystèmes d'Analyse (LAAS-MICA), Georgia Institute of Technology [Atlanta], Georgia Tech Lorraine [Metz], Ecole Nationale Supérieure des Arts et Metiers Metz-Georgia Institute of Technology [Atlanta]-Ecole Supérieure d'Electricité - SUPELEC (FRANCE)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), ANR-17-CE09-0024,LabPSi,Membranes de silicium poreux latérales comme solution générique de préparation d'échantillon et biodétection pour les laboratoires sur puce(2017), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Ecole Supérieure d'Electricité - SUPELEC (FRANCE)-Georgia Institute of Technology [Atlanta]-CentraleSupélec-Ecole Nationale Supérieure des Arts et Metiers Metz-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Microfluidics, 02 engineering and technology, 010402 general chemistry, Porous silicon, 01 natural sciences, [SPI]Engineering Sciences [physics], Planar, Astronomical interferometer, [CHIM]Chemical Sciences, Wafer, Electrical and Electronic Engineering, Instrumentation, business.industry, Metals and Alloys, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Interferometry, Membrane, Transducer, Optoelectronics, 0210 nano-technology, business
Abstract: International audience; Most porous silicon-based interferometric sensors targeting biosensing applications consist of vertical porous silicon layers created into a silicon wafer by electrochemical anodization and operate in a flow-over configuration. In this work, we present an alternative porous silicon interferometer based on porous silicon with horizontally oriented pores. This architecture permits the integration of flow-through porous silicon membranes within planar microfluidics. Fourier-transform infrared spectroscopy was used to obtain interference spectra from fabricated lateral porous silicon membranes and red shifts were observed upon filling microfluidic chips integrating the porous membranes with solvents of higher optical indices. This work proves that lateral porous silicon membranes are typical Fabry-Pérot interferometers with a sensitivity of more than 150 nm/RIU and a limit of detection less than 10−3 RIU, that is comparable to vertical porous silicon layers. Moreover, we have conducted simulation studies showing that the addition of Bragg mirrors on the membranes results in spectra with narrower fringes and lateral porous silicon interferometers with improved performances. After appropriate biofunctionalization of the porous silicon surface, lateral porous silicon membrane interferometers should offer alternative solutions for the development of porous silicon flow-through biosensors monolithically integrated on-chip.
Published: 2021
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24. Organosilica Nanoparticles for Gemcitabine Monophosphate Delivery in Cancer Cells

Author: Saher Rahmani, Jelena Budimir, Morgane Daurat, Yannick Guari, Laurence Raehm, Peter Hesemann, Sébastien Richeter, Clarence Charnay, Roza Bouchal, Magali Gary-Bobo, Nadir Bettache, Alia Akrout, Christophe Nguyen, Jean-Olivier Durand, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre de recherche en Biologie Cellulaire (CRBM), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: gemcitabine monophosphate, organosilica nanoparticles, drug delivery, cancer, ionosilica, Renewable Energy, Sustainability and the Environment, Chemistry, Energy Engineering and Power Technology, Nanoparticle, Cancer, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, medicine.disease, 01 natural sciences, Gemcitabine, 0104 chemical sciences, Biomaterials, Cancer cell, Drug delivery, Materials Chemistry, medicine, Cancer research, [CHIM.COOR]Chemical Sciences/Coordination chemistry, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, medicine.drug
Abstract: Organosilica nanoparticles hold great promise for nanomedicine applications. These nanoparticles are synthesized from polytrialkoxysilylated precursors without any silica source. In this work we present two kinds of organosilica nanoparticles with either amine or ammonium walls constituting their structure. Both types of nanoparticles are very efficient for gemcitabine monophosphate delivery, a small hydrophilic anticancer drug whose encapsulation is still a challenge. The nanoparticles are endocytosed by MCF-7 breast cancer cells as monitored by confocal microscopy. They are efficient and lead to 60% cancer cell death.
Published: 2019
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25. Periodic Mesoporous Ionosilica Nanoparticles for Green Light Photodynamic Therapy and Photochemical Internalization of siRNA

Author: Jean-Olivier Durand, Nadir Bettache, Peter Hesemann, Braham Mezghrani, Sébastien Richeter, Lamiaa M. A. Ali, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)
Subjects: Small interfering RNA, Materials science, medicine.medical_treatment, Nanoparticle, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Dynamic light scattering, medicine, Human Umbilical Vein Endothelial Cells, Humans, General Materials Science, Gene Silencing, RNA, Small Interfering, ComputingMilieux_MISCELLANEOUS, Cells, Cultured, Photosensitizing Agents, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Silicon Dioxide, Fluorescence, Porphyrin, 0104 chemical sciences, chemistry, Photochemotherapy, Biophysics, Nanoparticles, Nanocarriers, 0210 nano-technology, Phototoxicity
Abstract: We report periodic mesoporous ionosilica nanoparticles (PMINPs) as versatile nano-objects for imaging, photodynamic therapy (PDT), and efficient adsorption and delivery of small interfering RNA (siRNA) into breast cancer cells. In order to endow these nanoparticles with PDT and siRNA photochemical internalization (PCI) properties, a porphyrin derivative was integrated into the ionosilica framework. For this purpose, we synthesized PMINPs via hydrolysis-cocondensation procedures from oligosilylated ammonium and porphyrin precursors. The formation of these nano-objects was proved by transmission electron microscopy. The formed nanoparticles were then thoroughly characterized via solid-state NMR, nitrogen sorption, dynamic light scattering, and UV-vis and fluorescence spectroscopies. Our results indicate the formation of highly porous nanorods with a length of 108 ± 9 nm and a width of 54 ± 4 nm. A significant PDT effect of type I mechanism (95 ± 2.8% of cell death) was observed upon green light irradiation in nanoparticle-treated breast cancer cells, while the blue light irradiation caused a significant phototoxic effect in non-treated cells. Furthermore, PMINPs formed stable complexes with siRNA (up to 24 h), which were efficiently internalized into the cells after 4 h of incubation mostly with the energy-dependent endocytosis process. The PCI effect was obvious with green light irradiation and successfully led to 83 ± 1.1% silencing of the luciferase gene in luciferase-expressing breast cancer cells, while no gene silencing effect was observed with blue light irradiation. The present work highlights the high potential of porphyrin-doped PMINPs as multifunctional nanocarriers for nucleic acids, such as siRNA, with a triple ability to perform imaging, PDT, and PCI.
Published: 2021

26. Mesoporous silica adsorbents modified with amino polycarboxylate ligands – functional characteristics, health and environmental effects

Author: Ani Vardanyan, Gulaim A. Seisenbaeva, Lamiaa M. A. Ali, Vadim G. Kessler, Jean-Olivier Durand, Magali Gary-Bobo, Tetyana M. Budnyak, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)
Subjects: Environmental Engineering, Health, Toxicology and Mutagenesis, 0211 other engineering and technologies, 02 engineering and technology, 010501 environmental sciences, Ligands, 01 natural sciences, Adsorption, Environmental Chemistry, Animals, Humans, Waste Management and Disposal, Zebrafish, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, 021110 strategic, defence & security studies, Ligand, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Extraction (chemistry), Endothelial Cells, Sorption, Mesoporous silica, Silicon Dioxide, Pollution, Covalent bond, Surface modification, Mesoporous material, Nuclear chemistry
Abstract: A series of hybrid adsorbents were produced by surface modification with amino polycarboxylate ligands of industrially available microparticles (MP) of Kromasil® mesoporous nanostructured silica beads, bearing grafted amino propyl ligands. Produced materials, bearing covalently bonded functions as EDTA and TTHA, original Kromasil®, bearing amino propyl ligands, and bare particles, obtained by thermal treatment of Kromasil® in air, were characterized by SEM-EDS, AFM, FTIR, TGA and gas sorption techniques. Adsorption kinetics and capacity of surface-modified particles to adsorb Rare Earth Elements (REE), crucial for extraction in recycling processes, were evaluated under dynamic conditions, revealing specificity matching the ligand nature and the size of REE cations. A detailed comparison with earlier reported adsorbents for REE extraction was presented. The cytotoxicity was assessed using four different types of healthy cells, human skeletal muscles derived cells (SKMDC), fibroblast cells, macrophage cells (RAW264.7), and human umbilical vein endothelial cells (HUVECs), indicating lower toxicity of ligand-free MP than MP bearing amino poly-carboxylate functions. Internalization of the MP inside the cells and release of nitric oxide were observed. In addition, zebrafish embryos were exposed to high concentrations of MP and did not show any pronounced toxicity.
Published: 2021
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27. Synthesis of triethoxysilylated cyclen derivatives, grafting on magnetic mesoporous silica nanoparticles and application to metal ion adsorption

Author: Erwan Oliviero, Jean-Olivier Durand, Roser Pleixats, Clarence Charnay, Gulaim A. Seisenbaeva, Hao Li, Laurence Raehm, Mathilde Ménard, Ani Vardanyan, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Swedish University of Agricultural Sciences (SLU), Departament de Química [Barcelona] (UAB), and Universitat Autònoma de Barcelona (UAB)
Subjects: Solid-state chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Chemistry, General Chemical Engineering, Metal ions in aqueous solution, Nanoparticle, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Adsorption, Cyclen, Materials Chemistry, Click chemistry, Surface modification, 0210 nano-technology, Nuclear chemistry
Abstract: International audience; The synthesis through click chemistry of triethoxysilylated cyclen derivative-based ligands is described. Different methods were used such as the copper catalyzed Huisgen's reaction, or thiol-ene reaction for the functionalization of the cyclen scaffold with azidopropyltriethoxysilane or mercaptopropyltriethoxysilane, respectively. These ligands were then grafted on magnetic mesoporous silica nanoparticles (MMSN) for extraction and separation of Ni(II) and Co(II) metal ions from model solutions. The bare and ligand-modified MMSN materials revealed high adsorption capacity (1.0-2.13 mmol g À1) and quick adsorption kinetics, achieving over 80% of the total capacity in 1-2 hours.
Published: 2021
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28. Controlled synthesis and osmotic properties of ionosilica nanoparticles

Author: Philippe Trens, Alysson Duarte Rodrigues, Jean-Olivier Durand, Matthieu Jacob, Peter Hesemann, Benedicte Prelot, Véronique Gauchou, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Pôle d'Etude et de Recherche de Lacq [Total] (PERL), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)
Subjects: Chemistry, Forward osmosis, Ionic bonding, Nanoparticle, [CHIM.MATE]Chemical Sciences/Material chemistry, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Dynamic light scattering, Chemical engineering, Mechanics of Materials, Specific surface area, Ionic liquid, Particle, [CHIM]Chemical Sciences, General Materials Science, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract: Ionosilica nanoparticles are original ionic nano-objects at the interface of ionic liquids and mesoporous silica. Ionosilica nanoparticles' synthesis was achieved via sol-gel procedures exclusively from ionic trialkoxysilylated precursors without any silica source. However, the knowledge about the main synthesis parameters for a controlled synthesis of ionosilica nanoparticles remained fragmental so far. In this work, we present a systematic study for ionosilica nanoparticle formation starting from a virtually cationic silylated ammonium precursor. Due to their ionic nature, the behavior of ionic precursors considerably differs from that of conventional molecular silylated precursors. Here, we screened several reaction parameters for ionosilica nanoparticles’ formation such as solvent polarity, precursor and surfactant concentrations, and temperature. Our results allow controlling the textures and architectures of ionosilica nanoparticles in terms of porosity (specific surface area) and particle size. The formed ionosilica nanoparticles were thoroughly characterized by means of nitrogen sorption, elemental analysis, electron microscopies, dynamic light scattering (DLS), and solid state NMR measurements. Liquid 1H NMR spectroscopic measurements allowed monitoring the kinetics of the hydrolysis-polycondensation reactions. It appeared that the hydrolysis is relatively fast (30–120 min) whereas the polycondensation reaction requires more time (1–2 days). Finally, we used Forward Osmosis (FO-) experiments as a valuable tool to monitor a possible evolution of the nanoparticles in aqueous media. We observed that the ionosilica nanoparticles showed decreasing osmotic properties in successive FO-regeneration cycles. We attribute these results either to the exchange of the osmotically active halide anions or to particle agglomeration. Ionosilica nanoparticles therefore show limited long-term stability in aqueous media that limit their potential as draw solute in forward osmosis.
Published: 2021
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29. Quaternary Ammonium-Based Ionosilica Hydrogels as Draw Solutes in Forward Osmosis

Author: Matthieu Jacob, Peter Hesemann, Jean-Olivier Durand, Véronique Gauchou, Philippe Trens, Alysson Duarte Rodrigues, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and Pôle d'Etude et de Recherche de Lacq [Total] (PERL)
Subjects: Osmosis, Forward osmosis, Ultrafiltration, Pharmaceutical Science, Halide, 02 engineering and technology, Wastewater, 010402 general chemistry, 01 natural sciences, Article, 12. Responsible consumption, Analytical Chemistry, Water Purification, lcsh:QD241-441, chemistry.chemical_compound, lcsh:Organic chemistry, Drug Discovery, Osmotic pressure, [CHIM]Chemical Sciences, Ammonium, Physical and Theoretical Chemistry, Saline Waters, ComputingMilieux_MISCELLANEOUS, Ion exchange, forward osmosis, Organic Chemistry, draw solute, Hydrogels, 021001 nanoscience & nanotechnology, 6. Clean water, 0104 chemical sciences, Solvent, Quaternary Ammonium Compounds, ionosilica, chemistry, Chemical engineering, Chemistry (miscellaneous), Self-healing hydrogels, Molecular Medicine, hydrogel, 0210 nano-technology
Abstract: In the last few years, forward osmosis (FO) has attracted increasing interest as a sustainable technique for water desalination and wastewater treatment. However, FO remains as an immature process principally due to the lack of efficient and easily recyclable draw solutes. In this work, we report that ionosilica hydrogels based on quaternary ammonium halide ionosilica are efficient draw solutes in FO. Fluidic ionosilica hydrogels were obtained via hydrolysis-polycondensation reactions of a trisilylated quaternary ammonium precursor in slightly acidic water/ethanol solvent mixtures. The liquid-to-gel transition of the precursor and the kinetics of the formation of hydrogels were monitored by liquid NMR measurements. The formed hydrogels were shown to generate osmotic pressure up to 10.0 atm, indicating the potential of these hydrogels as efficient draw solutes in FO. Our results suggest that iodide anions are the osmotically active species in the system. Regeneration of the hydrogels via ultrafiltration (UF) was successfully achieved, allowing the development of a closed FO-UF process. However, the osmotic performances of the ionosilica hydrogels irreversibly decreased along the successive FO-UF cycles, probably due to anion exchange processes.
Published: 2020
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30. Nanodiamonds for bioapplications, recent developments

Author: Rabah Boukherroub, Nicolas Bondon, Laurence Raehm, Clarence Charnay, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Computer science, Biomedical Engineering, Antineoplastic Agents, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Nanodiamonds, Drug Delivery Systems, Anti-Infective Agents, Drug Development, Animals, Humans, [CHIM]Chemical Sciences, General Materials Science, Particle Size, Biomedicine, Drug Carriers, Wound Healing, business.industry, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, 3. Good health, Nanomedicine, 0210 nano-technology, business
Abstract: International audience; The world of biomedical research is in constant evolution, requiring more and more conditions and norms through pre-clinic and clinic studies. Nanodiamonds (NDs) with exceptional optical, thermal and mechanical properties emerged on the global scientific scene and recently gained more attention in biomedicine and bioanalysis fields. Many problematics have been deliberated to better understand their in vitro and in vivo efficiency and compatibility. Light was shed on their synthesis, modification and purification steps, as well as particle size and surface properties in order to find the most suitable operating conditions. In this review, we present the latest advances of NDs use in bioapplications. A large variety of subjects including anticancer and antimicrobial systems, wound healing and tissue engineering management tools, but also bioimaging and labeling probes are tackled. The key information resulting from these recent works were evidenced to make an overview of the potential features of NDs, with a special look on emerging therapeutic and diagnosis combinations.
Published: 2020
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31. Fluorescent periodic mesoporous organosilica nanoparticles dual-functionalized via click chemistry for two-photon photodynamic therapy in cells

Author: Marie Maynadier, Laurence Raehm, Jean-Olivier Durand, Xavier Cattoën, Marcel Garcia, Maxime Klausen, Sébastien Picard, Dina Aggad, Guillaume Clermont, Chiara Mauriello Jimenez, Mireille Blanchard-Desce, Jonas G. Croissant, Emilie Genin, Michel Wong Chi Man, Magali Gary-Bobo, Olivier Mongin, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (NEEL - OPTIMA), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Optique et Matériaux (OPTIMA )
Subjects: Fluorophore, Materials science, Singlet oxygen, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Biomedical Engineering, Nanoparticle, 02 engineering and technology, General Chemistry, General Medicine, 010402 general chemistry, 021001 nanoscience & nanotechnology, Photochemistry, 01 natural sciences, Fluorescence, 0104 chemical sciences, chemistry.chemical_compound, Mesoporous organosilica, chemistry, Click chemistry, General Materials Science, Photosensitizer, 0210 nano-technology, Mesoporous material, ComputingMilieux_MISCELLANEOUS
Abstract: The synthesis of ethenylene-based periodic mesoporous organosilica nanoparticles for two-photon imaging and photodynamic therapy of breast cancer cells is described. A dedicated two-photon absorbing fluorophore possessing four triethoxysilyl groups and having large two-photon absorption in the near IR region, and azidopropyltriethoxysilane were incorporated into the structure. The mesoporous nanoparticles of 100 nm diameter were further functionalized by means of click chemistry with a propargylated fluorescent bromo-quinoline photosensitizer able to generate singlet oxygen. The photophysical properties and two-photon absorption properties of the nanoparticles were investigated evidencing complementary contribution of the two dyes. Both dyes contribute to the two-photon absorption response of the mesoporous nanoparticles while efficient FRET from the two-photon fluorophore to the quinoline sensitizer is observed. The dual-functionalized nanoparticles were incubated with MCF-7 breast cancer cells. Two-photon confocal imaging demonstrated the endocytosis of the nanoparticles within cancer cells. Moreover, brief two-photon irradiation (3 scans of 1.57 s) at 760 nm at high laser power (3 W) was shown to induce 40% of cancer cell death demonstrating the potential of the dual-functionalized mesoporous organosilica nanoparticles for two-photon photodynamic therapy.
Published: 2020
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32. Influence of the synthetic method on the properties of two-photon-sensitive mesoporous silica nanoparticles

Author: Marie Maynadier, Laurence Raehm, Clarence Charnay, Michel Wong Chi Man, Vanja Stojanovic, Xavier Cattoën, Magali Gary-Bobo, Olivier Mongin, Jonas G. Croissant, Jean-Olivier Durand, Mireille Blanchard-Desce, Marcel Garcia, Vincent Hugues, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (NEEL - OPTIMA), Institut Néel (NEEL), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), NanoMedSyn (NMS), ANR-10-NANO-0022,MECHANANO,Nanomachines mécanisées pour l'activation à deux photons(2010), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (OPTIMA), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), and NanoMedSyn
Subjects: cross-section, Materials science, Mesoporous silica nanoparticles, Biomedical Engineering, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Two-photon excitation microscopy, Bromide, Organic chemistry, General Materials Science, Photosensitizer, Porosity, two-photon, Aqueous medium, [CHIM.ORGA]Chemical Sciences/Organic chemistry, imaging, General Chemistry, General Medicine, Mesoporous silica, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Chemical engineering, Spatial dispersion, cancer cells, 0210 nano-technology
Abstract: International audience; Herein we report the modulation of the properties of mesoporous silica nanoparticles (NPs) via various synthetic approaches. Three types of elaborations were compared, one in aqueous media at 25 °C, and the other two at 80 °C in water or in a water–ethanol mixture. For all these methods, an alkoxysilylated two-photon photosensitizer (2PS) was co-condensed with tetraethylorthosilicate (TEOS) in the presence of cetyltrimethylammonium bromide (CTAB), leading to five two-photon-sensitive mesoporous silica (M2PS) NPs. The M2PS NP porous structure could be tuned from radial to worm-like and MCM-41 types of organization. Besides, the 2PS precursor spatial dispersion was found to be highly dependent on both the 2PS initial concentration and the elaboration process. As a result, two-photon properties were modulated by the choice of the synthesis, the best results being found in aqueous media at 25 or 80 °C. Finally, the M2PS NPs were used for in vitro two-photon imaging of cancer cells.
Published: 2020
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33. Periodic Mesoporous Organosilica Nanoparticles with BOC Group, towards HIFU Responsive Agents

Author: Roser Pleixats, Clarence Charnay, Chantal Pichon, Laurence Raehm, Hao Li, Anthony Delalande, Jean-Olivier Durand, Carolina Gascó, Patrick Midoux, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Department of Chemistry, and Universitat Autònoma de Barcelona (UAB)
Subjects: CO2, Formic Acid Esters, Contrast Media, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Analytical Chemistry, lcsh:QD241-441, lcsh:Organic chemistry, Drug Discovery, Humans, Molecule, cancer, [CHIM]Chemical Sciences, Organosilicon Compounds, Physical and Theoretical Chemistry, Cancer, Drug Carriers, Nanotubes, Chemistry, Organic Chemistry, periodic mesoporous organosilica nanoparticles, HIFU, Carbon Dioxide, Silicon Dioxide, 021001 nanoscience & nanotechnology, Combinatorial chemistry, 3. Good health, 0104 chemical sciences, Mesoporous organosilica, Nanomedicine, Polymerization, Chemistry (miscellaneous), Click chemistry, Nanoparticles, Molecular Medicine, Click Chemistry, Nanorod, Periodic mesoporous organosilica nanoparticles, 0210 nano-technology, Mesoporous material, Porosity
Abstract: Periodic Mesoporous Organosilica Nanoparticles (PMONPs) are nanoparticles of high interest for nanomedicine applications. These nanoparticles are not composed of silica (SiO2). They belong to hybrid organic&ndash, inorganic systems. We considered using these nanoparticles for CO2 release as a contrast agent for High Intensity Focused Ultrasounds (HIFU). Three molecules (P1&ndash, P3) possessing two to four triethoxysilyl groups were synthesized through click chemistry. These molecules possess a tert-butoxycarbonyl (BOC) group whose cleavage in water at 90&ndash, 100 &deg, C releases CO2. Bis(triethoxysilyl)ethylene E was mixed with the molecules Pn (or not for P3) at a proportion of 90/10 to 75/25, and the polymerization triggered by the sol-gel procedure led to PMONPs. PMONPs were characterized by different techniques, and nanorods of 200&ndash, 300 nm were obtained. These nanorods were porous at a proportion of 90/10, but non-porous at 75/25. Alternatively, molecules P3 alone led to mesoporous nanoparticles of 100 nm diameter. The BOC group was stable, but it was cleaved at pH 1 in boiling water. Molecules possessing a BOC group were successfully used for the preparation of nanoparticles for CO2 release. The BOC group was stable and we did not observe release of CO2 under HIFU at lysosomal pH of 5.5. The pH needed to be adjusted to 1 in boiling water to cleave the BOC group. Nevertheless, the concept is interesting for HIFU theranostic agents.
Published: 2020
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34. Murine and Non-Human Primate Dendritic Cell Targeting Nanoparticles for in Vivo Generation of Regulatory T-Cells

Author: Robert P. Carroll, Patrick T. Coates, Juewan Kim, Chris Drogemuller, Nicolas H. Voelcker, Francis D. Kette, Jean-Olivier Durand, Sebastian O. Stead, Frédérique Cunin, Steven J. P. McInnes, Kisha N. Sivanathan, Eduardo J. Cueto-Díaz, Svjetlana Kireta, Stead, Sebastian O, Kireta, Svjetlana, McInnes, Steve JP, Kette, Francis D, Sivanathan, Kisha N, Kim, Juewan, Cueto-Diaz, Eduardo J, Cunin, Frederique, Durand, Jean Olivier, Drogemuller, Christopher J, Carroll, Robert P, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Mawson Institute, and University of South Australia
Subjects: Male, 0301 basic medicine, Immunoconjugates, General Physics and Astronomy, 02 engineering and technology, T-Lymphocytes, Regulatory, Drug Delivery Systems, General Materials Science, tolerance, biology, medicine.diagnostic_test, Chemistry, General Engineering, Antibodies, Monoclonal, FOXP3, Callithrix, 021001 nanoscience & nanotechnology, nanomedicine, 3. Good health, Cell biology, DC-SIGN, porous silicon, medicine.anatomical_structure, 0210 nano-technology, Immunosuppressive Agents, Silicon, Ovalbumin, medicine.drug_class, CD11c, Receptors, Cell Surface, Spleen, Monoclonal antibody, Flow cytometry, 03 medical and health sciences, In vivo, medicine, [CHIM]Chemical Sciences, Animals, Lectins, C-Type, Sirolimus, rapamycin, Dendritic Cells, Dendritic cell, regulatory T-cells, CD11c Antigen, Mice, Inbred C57BL, 030104 developmental biology, biology.protein, Nanoparticles, Cell Adhesion Molecules
Abstract: International audience; Porous silicon nanoparticles (pSiNP), modified to target dendritic cells (DC), provide an alternate strategy for the delivery of immunosuppressive drugs. Here, we aimed to develop a DC-targeting pSiNP displaying c-type lectin, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), and CD11c monoclonal antibodies. The in vivo tracking of these fluorescent DC-targeting nanoparticles was assessed in both C57BL/6 mice and common marmosets (Callithrix jacchus) by intravenous injection (20 mg/kg). Rapamycin and ovalbumin (OVA)323–339 peptide loaded pSiNP were employed to evaluate their ability to generate murine CD4+CD25+FoxP3+ regulatory T-cells in vivo within OVA sensitized mice. In vivo, pSiNP migrated to the liver, kidneys, lungs, and spleen in both mice and marmosets. Flow cytometry confirmed pSiNP uptake by splenic and peripheral blood DC when functionalized with targeting antibodies. C57BL/6 OVA sensitized mice injected with CD11c-pSiNP loaded with rapamycin + OVA323–339 produced a 5-fold higher number of splenic regulatory T-cells compared to control mice, at 40 days post-pSiNP injection. These results demonstrate the importance of the immobilized targeting antibodies to enhance cellular uptake and enable the in vivo generation of splenic regulatory T-cells.
Published: 2018
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35. Gemcitabine Delivery and Photodynamic Therapy in Cancer Cells via Porphyrin-Ethylene-Based Periodic Mesoporous Organosilica Nanoparticles

Author: Jean-Olivier Durand, Sébastien Richeter, Marie Maynadier, Jonas G. Croissant, Laure Lichon, Makhlouf Boufatit, Marcel Garcia, Laurence Raehm, Chiara Mauriello Jimenez, Danielle Laurencin, Dina Aggad, Soraya Dib, Shahad Alsaiari, Magali Gary-Bobo, and Niveen M. Khashab
Subjects: Materials science, Membrane permeability, medicine.medical_treatment, Energy Engineering and Power Technology, Nanotechnology, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Gemcitabine Hydrochloride, Biomaterials, chemistry.chemical_compound, Materials Chemistry, medicine, Photosensitizer, Renewable Energy, Sustainability and the Environment, 021001 nanoscience & nanotechnology, Porphyrin, Gemcitabine, 0104 chemical sciences, Mesoporous organosilica, chemistry, Cancer cell, Cancer research, 0210 nano-technology, medicine.drug
Abstract: Gemcitabine hydrochloride is an FDA-approved chemotherapeutic drug used in the treatment of various cancers. Several drawbacks of gemcitabine including its short in vivo half-life of 8-17 min associated with a rapid excretion by the kidneys and its poor membrane permeability have inspired research on a nanodelivery approach. In this study, we report ethylene-based periodic mesoporous organosilica nanoparticles (PMOs) for photodynamic therapy and the autonomous delivery of gemcitabine in cancer cells. Porphyrins were used as photosensitizers and were localized in the walls of the PMOs while a high loading capacity of gemcitabine was observed in the porous structure. Depending on the nature of the photosensitizer, and its aggregation state, we were able to perform one or two-photon photodynamic therapy. Two-photon excited photodynamic therapy combined with gemcitabine delivery led to a synergy and a very efficient cancer cell killing.
Published: 2017
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36. Synthesis of mesoporous silica nanoparticles and nanorods: Application to doxorubicin delivery

Author: Jean-Olivier Durand, Mokhtar Férid, Magali Gary-Bobo, Saher Rahmani, Clarence Charnay, Laure Lichon, and Marcel Garcia
Subjects: animal structures, Chromatography, Ethanol, Nanoparticle, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, chemistry, Cancer cell, Drug delivery, medicine, Particle, General Materials Science, Doxorubicin, Nanorod, sense organs, 0210 nano-technology, medicine.drug, Nuclear chemistry
Abstract: The synthesis and application of mesoporous silica nanoparticles (MSN) and mesoporous silica nanorods (MSNR) for drug delivery were described. MSN or MSNR were obtained by adjusting the amount of added cosolvent to the sol-gel solution. Therefore, the addition of ethanol (EtOH) has contributed to the control of the particle shape and to the structure of the mesoporosity. MSN and MSNR particles were then loaded with doxorubicin and incubated with MCF-7 breast cancer cells. MSN and MSNR particles were efficient in killing cancer cells but their behavior in drug delivery was altered on account of the difference in their morphology. MSN showed a burst release of doxorubicin in cells whereas MSNR showed a sustained delivery of the anti-cancer drug.
Published: 2017
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37. Preparation and characterization of novel mixed periodic mesoporous organosilica nanoparticles

Author: Hao Li, Clarence Charnay, Roser Pleixats, Laurence Raehm, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and Universitat Autònoma de Barcelona (UAB)
Subjects: Thermogravimetric analysis, Materials science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, sol-gel process, lcsh:Technology, 01 natural sciences, Article, Nanomaterials, Dynamic light scattering, 5dialkoxybenzoates, [CHIM]Chemical Sciences, General Materials Science, Sol-gel process, lcsh:Microscopy, lcsh:QC120-168.85, Sol-gel, disilylated tert-butyl 3,5-dialkoxybenzoates, lcsh:QH201-278.5, lcsh:T, periodic mesoporous organosilica nanoparticles, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Mesoporous organosilica, Disilylated tert -butyl 3,5-dialkoxybenzoates, lcsh:TA1-2040, disilylated tert-butyl 3, lcsh:Descriptive and experimental mechanics, Nanorod, lcsh:Electrical engineering. Electronics. Nuclear engineering, Periodic mesoporous organosilica nanoparticles, lcsh:Engineering (General). Civil engineering (General), 0210 nano-technology, Mesoporous material, lcsh:TK1-9971, Nuclear chemistry
Abstract: We report herein the preparation of mixed periodic mesoporous organosilica nanoparticles (E-Pn 75/25 and 90/10 PMO NPs) by sol-gel co-condensation of E-1,2-bis(triethoxysilyl)ethylene ((E)-BTSE or E) with previously synthesized disilylated tert-butyl 3,5-dialkoxybenzoates bearing either sulfide (precursor P1) or carbamate (precursor P2) functionalities in the linker. The syntheses were performed with cetyltrimethylammonium bromide (CTAB) as template in the presence of sodium hydroxide in water at 80 &deg, C. The nanomaterials have been characterized by Transmission Electron Microscopy (TEM), nitrogen-sorption measurements (BET), Dynamic Light Scattering (DLS), zeta-potential, Thermogravimetric Analysis (TGA), FTIR, 13C CP MAS NMR and small angle X-ray diffraction (p-XRD). All the nanomaterials were obtained as mesoporous rodlike-shape nanoparticles. Remarkably, E-Pn 90/10 PMO NPs presented high specific surface areas ranging from 700 to 970 m2g-1, comparable or even higher than pure E PMO nanorods. Moreover, XRD analyses showed an organized porosity for E-P1 90/10 PMO NPs typical for a hexagonal 2D symmetry. The other materials showed a worm-like mesoporosity.
Published: 2020
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38. Encapsulation of Upconversion Nanoparticles in Periodic Mesoporous Organosilicas

Author: Clarence Charnay, Christophe Nguyen, Dina Aggad, Manuel Ocaña, Magali Gary-Bobo, Rachid Mahiou, Lamiaa M. A. Ali, Erwan Oliveiro, Damien Boyer, Ana Isabel Becerro Nieto, Jean-Olivier Durand, Laurence Raehm, Nadège Francolon, Daniel González-Mancebo, Chiara Mauriello Jimenez, Saher Rahmani, Institut de Chimie de Clermont-Ferrand - Clermont Auvergne (ICCF), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Instituto de Ciencia de Materiales de Sevilla (ICMSE), Universidad de Sevilla / University of Sevilla-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut de Chimie de Clermont-Ferrand (ICCF), SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Agencia Estatal de Investigación (España), Agence Nationale de la Recherche (France), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Universidad de Sevilla-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), and Clot, Eric
Subjects: Materials science, Condensation polymer, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Analytical Chemistry, lcsh:QD241-441, Fluorides, Drug Delivery Systems, lcsh:Organic chemistry, TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY, [CHIM] Chemical Sciences, Drug Discovery, Technology, Pharmaceutical, cancer, [CHIM]Chemical Sciences, Organosilicon Compounds, Yttrium, Physical and Theoretical Chemistry, Ytterbium, ComputingMilieux_MISCELLANEOUS, Cancer, upconversion, Ethane, Nanotubes, Hydrolysis, Organic Chemistry, periodic mesoporous organosilica nanoparticles, 021001 nanoscience & nanotechnology, Photon upconversion, 0104 chemical sciences, Mesoporous organosilica, Nanomedicine, Chemical engineering, Chemistry (miscellaneous), Drug delivery, Molecular Medicine, Nanoparticles, Nanorod, Periodic mesoporous organosilica nanoparticles, 0210 nano-technology, Mesoporous material, Upconversion, Erbium
Abstract: 1) Background: Nanomedicine has recently emerged as a promising field, particularly for cancer theranostics. In this context, nanoparticles designed for imaging and therapeutic applications are of interest. We, therefore, studied the encapsulation of upconverting nanoparticles in mesoporous organosilica nanoparticles. Indeed, mesoporous organosilica nanoparticles have been shown to be very efficient for drug delivery, and upconverting nanoparticles are interesting for near-infrared and X-ray computed tomography imaging, depending on the matrix used. (2) Methods: Two different upconverting-based nanoparticles were synthesized with Yb3+-Er3+ as the upconverting system and NaYF4 or BaLuF5 as the matrix. The encapsulation of these nanoparticles was studied through the sol-gel procedure with bis(triethoxysilyl)ethylene and bis(triethoxysilyl)ethane in the presence of CTAB. (3) Results: with bis(triethoxysilyl)ethylene, BaLuF5: Yb3+-Er3+, nanoparticles were not encapsulated, but anchored on the surface of the obtained mesoporous nanorods BaLuF5: Yb3+-Er3+@Ethylene. With bis(triethoxysilyl)ethane, BaLuF5: Yb3+-Er3+ and NaYF4: Yb3+-Er3+nanoparticles were encapsulated in the mesoporous cubic structure leading to BaLuF5: Yb3+-Er3+@Ethane and NaYF4: Yb3+-Er3+@Ethane, respectively. (4) Conclusions: upconversion nanoparticles were located on the surface of mesoporous nanorods obtained by hydrolysis polycondensation of bis(triethoxysilyl)ethylene, whereas encapsulation occurred with bis(triethoxysilyl)ethane. The later nanoparticles NaYF4: Yb3+-Er3+@Ethane or BaLuF5: Yb3+-Er3+@Ethane were promising for applications with cancer cell imaging or X-ray-computed tomography respectively., This research was funded by ANR NanoptPDT. SR thanks the Erasmus Mundus program for a grant. This work has been supported by the Spanish Ministry of Science, Innovation and Universities (RTI2018-094426-B-I00).
Published: 2019
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39. Phthalocyanine-based mesoporous organosilica nanoparticles: NIR photodynamic efficiency and siRNA photochemical internalization

Author: Erwan Oliviero, Jean-Olivier Durand, Gülçin Ekineker, Vefa Ahsen, Sofia Dominguez Gil, Anastasia Godefroy, Ümit İşci, Sümeyra Bayır, Fabienne Dumoulin, Clarence Charnay, Christophe Nguyen, Laurence Raehm, Morgane Daurat, Magali Gary-Bobo, Gebze Technical University, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Department of chemistry - Gebze Technical University, Gebze Teknik Üniversitesi [Gebze], and NanoMedSyn
Subjects: Indoles, Cell Survival, Infrared Rays, Surface Properties, Static Electricity, Nanoparticle, Isoindoles, 010402 general chemistry, Photochemistry, 01 natural sciences, Catalysis, chemistry.chemical_compound, Static electricity, Organometallic Compounds, Materials Chemistry, Humans, [CHIM]Chemical Sciences, Luciferase, Irradiation, RNA, Small Interfering, Luciferases, Cetrimonium, 010405 organic chemistry, Metals and Alloys, General Chemistry, Transfection, Silanes, Photochemical Processes, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Mesoporous organosilica, Photochemotherapy, chemistry, Zinc Compounds, MCF-7 Cells, Ceramics and Composites, Phthalocyanine, Nanoparticles, Phototoxicity, Porosity
Abstract: International audience; Mesoporous organosilica nanoparticles (PHT-PMO) have been prepared from an octa-triethoxysilylated Zn phthalocyanine precursor. These PHT-PMO nanoparticles had no dark toxicity but high phototoxicity when irradiated at 650 nm, and remarkable near-infrared phototoxicity when excited at 760 and 810 nm. The PHT-PMO were then aminated to promote electrostatic complexation with siRNA. Transfection experiments were performed upon NIR irradiation and photochemical internalization was very efficient, leading to 65% luciferase extinction in MCF-7 cancer cells expressing stable luciferase.
Published: 2019
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40. Efficient Photodynamic Therapy of Prostate Cancer Cells through an Improved Targeting of the Cation-Independent Mannose 6-Phosphate Receptor

Author: Marcel Garcia, Jean-Olivier Durand, Marie Maynadier, Elise Bouffard, Laurence Raehm, Khaled El Cheikh, Chiara Mauriello Jimenez, Christophe Nguyen, Alain Morère, Magali Gary-Bobo, Ilaria Basile, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and NanoMedSyn
Subjects: Male, mannose 6-phosphate analogues, medicine.medical_treatment, Mannose, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, Endocytosis, 01 natural sciences, Receptor, IGF Type 2, Catalysis, lcsh:Chemistry, Inorganic Chemistry, chemistry.chemical_compound, Prostate cancer, Cell Line, Tumor, binding affinity, medicine, Humans, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Receptor, mesoporous silica nanoparticles, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, Mannosephosphates, Communication, Organic Chemistry, Prostatic Neoplasms, General Medicine, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, medicine.disease, 0104 chemical sciences, Computer Science Applications, Cation-independent mannose-6 phosphate receptor, lcsh:Biology (General), lcsh:QD1-999, Photochemotherapy, chemistry, photodynamic therapy, Cancer research, Nanoparticles, 0210 nano-technology
Abstract: This article belongs to the Special Issue Insights into Photodynamic TherapyThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).; International audience; The aim of the present work is the development of highly efficient targeting molecules to specifically address mesoporous silica nanoparticles (MSNs) designed for the photodynamic therapy (PDT) of prostate cancer. We chose the strategy to develop a novel compound that allows the improvement of the targeting of the cation-independent mannose 6-phosphate receptor, which is overexpressed in prostate cancer. This original sugar, a dimannoside-carboxylate (M6C-Man) grafted on the surface of MSN for PDT applications, leads to a higher endocytosis and thus increases the efficacy of MSNs. View Full-TextKeywords: mannose 6-phosphate analogues; binding affinity; mesoporous silica nanoparticles; photodynamic therapy
Published: 2019
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41. Porphyrin‐based bridged silsesquioxane nanoparticles for targeted two‐photon photodynamic therapy of zebrafish xenografted with human tumor

Author: Magali Gary-Bobo, Denis Durand, Makhlouf Boufatit, Soraya Dib, Jean-Olivier Durand, Ahmed Lakrafi, Alain Morère, Sofia Dominguez Gil, Vincent Chaleix, Dina Aggad, Nadir Bettache, Karim Bouchmella, Michel Wong Chi Man, Vincent Sol, Laurence Raehm, Guillaume Hery, Xavier Cattoën, Khaled El Cheikh, Chiara Mauriello Jimenez, Christophe Nguyen, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), CHU Pontchaillou [Rennes], Laboratoire de Chimie des Substances Naturelles (LCSN), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB), Optique et Matériaux (OPTIMA ), Institut Néel (NEEL), and Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Cancer Research, Porphyrins, medicine.medical_treatment, Nanoparticle, Breast Neoplasms, Photodynamic therapy, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 02 engineering and technology, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, 01 natural sciences, Theranostic Nanomedicine, chemistry.chemical_compound, Two-photon excitation microscopy, In vivo, Cell Line, Tumor, medicine, Animals, Humans, Zebrafish, two-photon excitation, Photosensitizing Agents, bridged silsesquioxane nanoparticles, Lasers, human tumor targeting, Original Articles, Silanes, 021001 nanoscience & nanotechnology, Xenograft Model Antitumor Assays, Porphyrin, 3. Good health, 0104 chemical sciences, Microscopy, Fluorescence, Multiphoton, photodynamic therapy, Photochemotherapy, Oncology, chemistry, Injections, Intravenous, Cancer cell, Click chemistry, Biophysics, Nanoparticles, Nanomedicine, Female, 0210 nano-technology
Abstract: International audience; Background: Bridged silsesquioxane nanoparticles (BSNs) recently described represent a new class of nanoparticles exhibiting versatile applications and particularly a strong potential for nanomedicine.Aims: In this work, we describe the synthesis of BSNs from an octasilylated functional porphyrin precursor (PORBSNs) efficiently obtained through a click reaction. These innovative and very small-sized nanoparticles were functionalized with PEG and mannose (PORBSNs-mannose) in order to target breast tumors in vivo. Methods and Results: The structure of these nanoparticles is constituted of porphyrins J aggregates that allow two-photon spatiotemporal excitation of the nanoparticles. The therapeutic potential of such photoactivable nanoparticles was first studied in vitro, in human breast cancer cells in culture and then in vivo on zebrafish embryos bearing human tumors. These animal models were intravenously injected with 5 nL of a solution containing PORBSNs-mannose. An hour and half after the injection of photoactivable and targeted nanoparticles, the tumor areas were excited for few seconds with a two-photon beam induced focused laser. We observed strong tumor size decrease, with the involvement of apoptosis pathway activation.onclusion: We demonstrated the high targeting, imaging, and therapeutic potential of PORBSNs-mannose injected in the blood stream of zebrafish xenografted with human tumors.
Published: 2019
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42. Degradable gold core–mesoporous organosilica shell nanoparticles for two-photon imaging and gemcitabine monophosphate delivery

Author: Saher Rahmani, Arnaud Chaix, Dina Aggad, Phuong Hoang, Niveen M. Khashab, Marcel Garcia, Magali Gary-Bobo, Basem Moosa, Clarence Charnay, Jean-Olivier Durand, and Abdulaziz Almalik
Subjects: Materials science, Process Chemistry and Technology, Biomedical Engineering, Energy Engineering and Power Technology, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Industrial and Manufacturing Engineering, Gemcitabine, 0104 chemical sciences, Mesoporous organosilica, Two-photon excitation microscopy, Chemistry (miscellaneous), Materials Chemistry, medicine, Chemical Engineering (miscellaneous), Nanomedicine, 0210 nano-technology, Luminescence, Gold core, medicine.drug
Abstract: The synthesis of degradable gold core–mesoporous organosilica shell nanoparticles is described. The nanoparticles were very efficient for two-photon luminescence imaging of cancer cells and for in vitro gemcitabine monophosphate delivery, allowing promising theranostic applications in the nanomedicine field.
Published: 2017
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43. Porphyrin- or phthalocyanine-bridged silsesquioxane nanoparticles for two-photon photodynamic therapy or photoacoustic imaging

Author: Vefa Ahsen, Dina Aggad, Jean-Luc Coll, Serkan Alpugan, Michel Wong Chi Man, Marcel Garcia, Marie Maynadier, Laurence Raehm, Deniz Kutlu Tarakci, Philippe Maillard, Fabienne Dumoulin, Clarence Charnay, Jean-Olivier Durand, Maxime Henry, Magali Gary-Bobo, Xavier Cattoën, Véronique Josserand, Chiara Mauriello-Jimenez, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Optique et Matériaux (OPTIMA ), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Department of chemistry - Gebze Technical University, Gebze Teknik Üniversitesi [Gebze], Chimie, Modélisation et Imagerie pour la Biologie [Orsay], Institut de Chimie du CNRS (INC)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], and NanoMedSyn
Subjects: Materials science, [CHIM.ORGA]Chemical Sciences/Organic chemistry, medicine.medical_treatment, Nanoparticle, Photodynamic therapy, [CHIM.MATE]Chemical Sciences/Material chemistry, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Photochemistry, 01 natural sciences, Porphyrin, Fluorescence, Silsesquioxane, 0104 chemical sciences, 3. Good health, chemistry.chemical_compound, chemistry, Two-photon excitation microscopy, Excited state, Phthalocyanine, medicine, General Materials Science, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract: Porphyrin- or phthalocyanine-bridged silsesquioxane nanoparticles (BSPOR and BSPHT) were prepared. Their endocytosis in MCF-7 cancer cells was shown with two-photon excited fluorescence (TPEF) imaging. With two-photon excited photodynamic therapy (TPE-PDT), BSPOR was more phototoxic than BSPHT, which in contrast displayed a very high signal for photoacoustic imaging in mice.
Published: 2017
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44. Cover Feature: Synthesis of Cyclen‐Functionalized Ethenylene‐Based Periodic Mesoporous Organosilica Nanoparticles and Metal‐Ion Adsorption Studies (ChemNanoMat 11/2020)

Author: Laurence Raehm, Roser Pleixats, Jean-Olivier Durand, Hao Li, Gulaim A. Seisenbaeva, Ani Vardanyan, and Clarence Charnay
Subjects: Materials science, Renewable Energy, Sustainability and the Environment, Energy Engineering and Power Technology, Nanoparticle, Metal adsorption, Biomaterials, chemistry.chemical_compound, Mesoporous organosilica, Cyclen, chemistry, Chemical engineering, Metal ion adsorption, Feature synthesis, Materials Chemistry, Cover (algebra)
Published: 2020
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45. Polythiophenes with Cationic Phosphonium Groups as Vectors for Imaging, siRNA Delivery, and Photodynamic Therapy

Author: Mathieu Surin, Jean-Olivier Durand, Philippe Arnoux, Christophe Nguyen, Morgane Daurat, Céline Frochot, Denis Durand, Karim Bouchmella, Bauyrzhan Myrzakhmetov, Sébastien Richeter, Clément Kotras, Magali Gary-Bobo, Laure Lichon, Lamiaa M. A. Ali, Sébastien Clément, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), NanoMedSyn, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre d'Innovation et de Recherche en Matériaux Polymères (CIRMAP), and Université de Mons (UMons)
Subjects: polythiophenes, siRNA delivery, General Chemical Engineering, medicine.medical_treatment, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, law.invention, lcsh:Chemistry, chemistry.chemical_compound, combined therapy, Confocal microscopy, law, Amphiphile, conjugated polyelectrolyte, medicine, [CHIM]Chemical Sciences, General Materials Science, Luciferase, chemistry.chemical_classification, Reactive oxygen species, Singlet oxygen, Cationic polymerization, imaging, 021001 nanoscience & nanotechnology, Fluorescence, 0104 chemical sciences, 3. Good health, lcsh:QD1-999, photodynamic therapy, chemistry, Biophysics, 0210 nano-technology
Abstract: In this work, we exploit the versatile function of cationic phosphonium-conjugated polythiophenes to develop multifunctional platforms for imaging and combined therapy (siRNA delivery and photodynamic therapy). The photophysical properties (absorption, emission and light-induced generation of singlet oxygen) of these cationic polythiophenes were found to be sensitive to molecular weight. Upon light irradiation, low molecular weight cationic polythiophenes were able to light-sensitize surrounding oxygen into reactive oxygen species (ROS) while the highest were not due to its aggregation in aqueous media. These polymers are also fluorescent, allowing one to visualize their intracellular location through confocal microscopy. The most promising polymers were then used as vectors for siRNA delivery. Due to their cationic and amphipathic features, these polymers were found to effectively self-assemble with siRNA targeting the luciferase gene and deliver it in MDA-MB-231 cancer cells expressing luciferase, leading to 30&ndash, 50% of the gene-silencing effect. In parallel, the photodynamic therapy (PDT) activity of these cationic polymers was restored after siRNA delivery, demonstrating their potential for combined PDT and gene therapy.
Published: 2020
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46. Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

Author: Laurence Raehm, Laure Lichon, Morgane Daurat, Clarence Charnay, Marcel Garcia, Dina Aggad, Magali Gary-Bobo, Jean-Olivier Durand, Mylene Sejalon, Eric Vivès, Jelena Budimir, Saher Rahmani, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Dispersity, Pharmaceutical Science, Nanoparticle, Peptide, Breast Neoplasms, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, pepstatin A, Analytical Chemistry, lcsh:QD241-441, chemistry.chemical_compound, Drug Delivery Systems, lcsh:Organic chemistry, Cell Line, Tumor, Drug Discovery, Pepstatins, Humans, cancer, [CHIM]Chemical Sciences, mesoporous silica nanoparticles, mesoporous organosilica nanoparticles, Physical and Theoretical Chemistry, chemistry.chemical_classification, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Organic Chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Silicon Dioxide, 0104 chemical sciences, 3. Good health, Membrane, Chemical engineering, Chemistry (miscellaneous), Cancer cell, Molecular Medicine, Nanomedicine, Nanoparticles, Female, 0210 nano-technology, Porosity, Pepstatin
Abstract: International audience; (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol-gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.
Published: 2019
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47. Amino-acid functionalized porous silicon nanoparticles for the delivery of pDNA

Author: Arnaud Chaix. Eduardo Cueto-Diaz, Anthony Delalande, Nikola Knezevic, Patrick Midoux, Jean-Olivier Durand, Chantal Pichon and Frederique Cunin
Published: 2019
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48. Degradable Hollow Organosilica Nanoparticles for Antibacterial Activity

Author: Jean-Olivier Durand, Philippe Trens, Saher Rahmani, Karim Bouchmella, Laurence Raehm, Mateus Borba Cardoso, Clarence Charnay, Jelena Budimir, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), University of Göttingen - Georg-August-Universität Göttingen, Laboratório Nacional de Luz Sìncrotron (LNLS), and Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)
Subjects: Chemistry, Methylamine, General Chemical Engineering, Dispersity, Nanoparticle, 02 engineering and technology, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, organosilica nanoparticles, antibacterial activity, 01 natural sciences, 0104 chemical sciences, lcsh:Chemistry, chemistry.chemical_compound, lcsh:QD1-999, 0210 nano-technology, Antibacterial activity, ComputingMilieux_MISCELLANEOUS, Nuclear chemistry
Abstract: The synthesis of hollow organosilica nanoparticles is described from bis triethoxysilylpropyldisulfide and bis(trimethoxylsilylpropyl)methylamine. The nanoparticles were monodisperse with a mean diameter of 90 nm, and the adsorption−desorption properties of these nanoparticles were investigated with nitrogen at 77 K, n-hexane, and water. The nanoparticles were nonporous at 77 K as shown by N2 sorption analyses. n-Hexane interacted reversibly through physisorption with the nanoparticles, whereas water showed a strong nonreversible interaction, probably because of chemisorption with nitrogen atom sites. Amoxicillin antibiotic was loaded with these nanoparticles and the loading capacity was 30%. Ag+ ions were also adsorbed with amoxicillin, and the multifunctional nanoparticles were efficient in inhibiting bacterial growth with a synergy between the antibiotic delivery and Ag+ release.
Published: 2019
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49. Mesoporous silica nanoparticles in recent photodynamic therapy applications

Author: Jean-Olivier Durand, Rabah Boukherroub, Sébastien Richeter, Laurence Raehm, Alexandre Barras, Sumeyra Bayir, Sabine Szunerits, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Department of Chemistry,Gebze Technical UniversityP. K.:141, 41400 Gebze, Kocaeli (Turkey), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Surface Properties, medicine.medical_treatment, Nanoparticle, Nanotechnology, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Alzheimer Disease, Neoplasms, medicine, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Particle Size, Photosensitizing Agents, Bacteria, Mesoporous silica, 021001 nanoscience & nanotechnology, Atherosclerosis, Silicon Dioxide, 0104 chemical sciences, 3. Good health, Photochemotherapy, Nanoparticles, 0210 nano-technology, Porosity
Abstract: International audience; In this review, the use of mesoporous silica nanoparticles for photodynamic therapy (PDT) applications is described for the year 2017. Since the pioneering work in 2009, nanosystems involving mesoporous silica nanoparticles have gained in complexity with a sophisticated core–shell system able to perform multi-imaging and multi-therapies, not only for cancer diseases but also for anti-microbial therapy, atherosclerosis, or Alzheimer disease. Near-infrared, excitation light based on up-converting systems, X-rays or persistent luminescent systems are described for deeper tissue treatments.
Published: 2018
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50. Hollow Organosilica Nanoparticles for Drug Delivery

Author: Christophe Nguyen, Saher Rahmani, Jelena Budimir, Alia Akrout, Morgane Daurat, Clarence Charnay, Magali Gary-Bobo, Dina Aggad, Laurence Raehm, Anastasia Godefroy, Hanene Largot, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Nanomedsyn
Subjects: Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Gemcitabine Hydrochloride, medicine, [CHIM]Chemical Sciences, Microemulsion, skin and connective tissue diseases, Chemistry, digestive, oral, and skin physiology, technology, industry, and agriculture, General Chemistry, 021001 nanoscience & nanotechnology, Uniform size, Gemcitabine, 0104 chemical sciences, Methotrexate, MCF-7 breast cancer cells, Organosilica Nanoparticles, Drug delivery, Breast cancer cells, 0210 nano-technology, medicine.drug, Nuclear chemistry
Abstract: International audience; The sol‐gel synthesis of hollow organosilica nanoparticles incorporating amino groups with uniform size are described. These nanoparticles were successfully prepared via a microemulsion method. Then, the hollow nanoparticles were loaded with gemcitabine hydrochloride or methotrexate and studied in MCF‐7 breast cancer cells.
Published: 2018
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