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Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

Authors :
Saher Rahmani
Jelena Budimir
Mylene Sejalon
Morgane Daurat
Dina Aggad
Eric Vivès
Laurence Raehm
Marcel Garcia
Laure Lichon
Magali Gary-Bobo
Jean-Olivier Durand
Clarence Charnay
Source :
Molecules, Vol 24, Iss 2, p 332 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.

Details

Language :
English
ISSN :
14203049 and 28440684
Volume :
24
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Molecules
Publication Type :
Academic Journal
Accession number :
edsdoj.761bfabb559d49b2844068495650054f
Document Type :
article
Full Text :
https://doi.org/10.3390/molecules24020332