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Papuan orchid species have triggered orchid enthusiasts to be hunted for many purposes such as ornamental plants, medicinal plants and even to breeding program. This intention has encouraged locals who have the resources to trade orchids and extracted from their natural habitat without taking into consideration on how to sustain this resources for the future. Efforts should be conducted in order to enlighten the community not only to preserve this resources but also to encourage locals on how to gain benefit economically using orchids as income generate to support their daily life. Adding to this attempts, extension program such as using materials to create media to grow orchids from coconut waste and sago disposal as basic materials. We employed extension program, focus group discussion, short training course and practicum during the implementation of the activities in each location. Six sites were chosen where the orchid growers and nurseries run by locals and communities living in reserve and non-reserve areas. Five sites located in Jayapura Regency, Jayapura City, Keerom Regency, Biak Regency of Papua Province and Manokwari Regency at West Papua Province. Materials to create media to grow orchids comes from coconut waste and sago disposal as basic materials. Wires and metal screen were used to make frame for potting. We also use tools such as clipper to cut wire. All participants were actively showing their enthusiast to gain knowledge during the course. Some have ask about species name whereas others addressing question of how to reproduce the orchids as well as how to maintain the orchids using home made pot.Keywords: Extension program; community empowerment; organic waste; orchids

A tree may receive compression and flexure combination, and the structural analysis governed by the building code may be capable of estimating the tree’s safety in the built environment. This study proposed to refer to the building code to check the tree dimension adequacy resisting the load. This study simplified the case by focusing only on the self-weight and ignoring the external loads; therefore, the buckling analysis of a slender tapered round column subjected to compression is advocated. Buckling occurs when the tree’s structure can no longer maintain its original shape. Euler and Ylinen’s buckling stress analysis (Method 1) calculated tree safety with a 95% confidence level. This study also applied the Greenhill formula (Method 2) to determine the critical height of a tree receiving the stem weight, then modified it to include the crown weight (Method 3). The three methods calculated the critical height to determine the safety factor (Sf), that is, the ratio of the actual tree height (H) to the 95% confidence level estimated critical height (Hcr). The safety factors were then categorized as unsafe (Sf < 1.00), safe (1.00 < Sf < 1.645), and very safe (1.645 < Sf). This study demonstrated that Method 1 is the most reliable and applicable among other methods. Method 1 resulted in no unsafe trees, 10 safe trees, and 13 very safe trees among the observed excurrent agathis (Agathis dammara). Meanwhile, among the decurrent rain trees (Samanea saman (Jacq.) Merr), 5, 31, and 14 were unsafe, safe, and very safe, respectively.

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

Hartoyo APP, Khairunnisa S, Pamoengkas P, Solikhin A, Supriyanto, Siregar IZ, Prasetyo LB, Istomo. 2022. Estimating carbon stocks of three traditional agroforestry systems and their relationships with tree diversity and stand density. Biodiversitas 23: 6137-6146. One of the important sectors as a strategy for climate resilience and low carbon development in Indonesia is Forestry and Other Land Uses (FOLU) including the agroforestry sector. Carbon stock and tree diversity in an ecosystem are dynamic over time and still need to be investigated. This study was aimed: i) to estimate aboveground carbon stocks in the traditional agroforestry of Mount Halimun Salak National Park (MHSNP), in Kampung Birang and Kampung Merabu, East Kalimantan, as well as ii) to analyze the correlation between carbon stock with tree diversity and stand density in traditional agroforestry of Mount Halimun Salak National Park (MHSNP), in Kampung Birang and Kampung Merabu, East Kalimantan. The total plots were 75 plots (a plot size of 50x50 m), and carbon stock estimation was focused on pole and tree levels. The average carbon stock in the traditional zone of MHSNP, Kampung Birang, and Kampung Merabu respectively was 51.02 Mg C/ha, 96.25 Mg C/ha, and 82.17 Mg C/ha. The carbon stock in traditional agroforestry of all study sites was very significantly affected by the basal area (m2/ha) (p-val

The successful isolation of DNA (Deoxyribonucleic Acid) is essential for the investigation process of forestry molecular genetics. Samples used are usually retrieved either from soft or juvenile plant organs because of their excellent DNA source. However, in certain cases, aforesaid samples are hard to obtain, as for forensic purposes. Alternatively, woods possess potential as alternative source of DNA whose extraction method has been developed with varying degrees of success. However, to date, effectiveness on tropical wood grown in Indonesia has not been widely reported. Therefore, objective of this study was to compare the results of DNA isolation of various dried and fresh wood samples by using two isolation methods: Cetyl Trimethyl Ammonium Bromide (CTAB) and Qiagen DNeasy Plant Mini Kit (QDPMK). Extraction results were visualized in agarose gels and quantified using Nanophotometer NP80 Implen which were then amplified using two universal primers: ITS and rbcL for detecting DNA signals. Extraction results from dried wood indicated no visualization in the gel, while fresh wood samples showed thick smeared bands on both extraction methods. Quantity test results denoted higher concentration in CTAB-extracted samples compared to samples extracted using QDPMK, in both types of samples, even though both resulted in optical density ratios outside the range of purity (λ260/280: 1,8–2,0 and λ260/230: 2,0, respectively). Success rates of ITS and rbcL primary amplification in dried wood samples were quite low yet outputs from the two methods did not differ significantly. Meanwhile, outcome of ITS and rbcL amplification on fresh wood samples had a fairly high success.

The pin-penetration device is a minimally destructive instrument that is widely used to estimate the physical properties of wood, e.g., density, with advantages such as reduced testing times, low costs, and fewer defects induced in the wood being tested. In this study, such a device was used on 25 Indonesian hardwood species with a strength class (SC) distribution from SC II to IV (according to the Indonesian classification of wood SCs). Tests were conducted on three different orthogonal planes, i.e., cross-sectional, radial, and tangential planes. The wood density ranged from 0.28 to 0.88 g/cm3, and the specific gravity was 0.25 to 0.76. The cross-sectional plane penetration depth of the wood was significantly greater than that of either the radial or tangential plane, whereas the pin penetration values of the radial and tangential planes were not significantly different. A prediction model for predicting the density and specific gravity of wood via pin penetration showed a significant regression. Thus, the use of a pin-penetration device was found to be suitable for estimating wood density and specific gravity in a range of SCs of tropical wood species regardless of species.

Tree diversity in Asia's tropical and subtropical forests is central to nature-based solutions. Species vulnerability to multiple threats, which affect provision of ecosystem services, is poorly understood. We conducted a region-wide, spatially explicit assessment of the vulnerability of 63 socioeconomically important tree species to overexploitation, fire, overgrazing, habitat conversion, and climate change. Trees were selected for assessment from national priority lists, and selections were validated by an expert network representing 20 countries. We used Maxent suitability modeling to predict species distribution ranges, freely accessible spatial data sets to map threat exposures, and functional traits to estimate threat sensitivities. Species-specific vulnerability maps were created as the product of exposure maps and sensitivity estimates. Based on vulnerability to current threats and climate change, we identified priority areas for conservation and restoration. Overall, 74% of the most important areas for conservation of these trees fell outside protected areas, and all species were severely threatened across an average of 47% of their native ranges. The most imminent threats were overexploitation and habitat conversion; populations were severely threatened by these factors in an average of 24% and 16% of their ranges, respectively. Our model predicted limited overall climate change impacts, although some study species were likely to lose over 15% of their habitat by 2050 due to climate change. We pinpointed specific natural areas in Borneo rain forests as hotspots for in situ conservation of forest genetic resources, more than 82% of which fell outside designated protected areas. We also identified degraded areas in Western Ghats, Indochina dry forests, and Sumatran rain forests as hotspots for restoration, where planting or assisted natural regeneration will help conserve these species, and croplands in southern India and Thailand as potentially important agroforestry options. Our results highlight the need for regionally coordinated action for effective conservation and restoration.Especies de Árboles Valoradas y Amenazadas de Asia Tropical y Subtropical Resumen La diversidad de árboles en los bosques tropicales y subtropicales de Asia es un eje central para las soluciones basadas en la naturaleza. La vulnerabilidad de las especies ante las múltiples amenazas, las cuales afectan el suministro de servicios ambientales, es un tema poco comprendido. Realizamos una evaluación regional espacialmente explícita de la vulnerabilidad de 63 especies de árboles de importancia socioeconómica ante la sobreexplotación, incendios, sobrepastoreo, conversión del hábitat y cambio climático. Los árboles se seleccionaron para su evaluación a partir de listas nacionales de prioridades, y las selecciones fueron validadas por una red de expertos de 20 países. Usamos el modelado de idoneidad Maxent para predecir el rango de distribución de las especies, conjuntos de datos espaciales de libre acceso para mapear la exposición a las amenazas y rasgos funcionales para estimar la susceptibilidad a las amenazas. Con base en la vulnerabilidad a las amenazas actuales y al cambio climático, identificamos las áreas prioritarias para su conservación y restauración. En general, el 74% de las áreas más importantes para la conservación de estos árboles quedó fuera de las áreas protegidas y todas las especies estaban seriamente amenazadas en promedio en el 47% de su distribución nativa. Las amenazas más inminentes fueron la sobreexplotación y la conversión del hábitat; las poblaciones estuvieron seriamente amenazadas por estos factores en promedio en el 24% y 16% de su distribución, respectivamente. Nuestro modelo predijo un impacto general limitado del cambio climático, aunque algunas especies estudiadas tuvieron la probabilidad de perder más del 15% de su hábitat para el 2050 debido a este factor. Identificamos áreas naturales específicas en las selvas de Borneo como puntos calientes para la conservación in situ de los recursos genéticos forestales, más del 82% de los cuales estaban fuera de las áreas protegidas designadas. También identificamos áreas degradadas en los Ghats Occidentales, los bosques secos de Indochina y las selvas de Sumatra como puntos calientes para la restauración, en donde la siembra o la regeneración natural asistida ayudarán a conservar estas especies. Además, identificamos campos de cultivo al sur de India y Tailandia como potenciales opciones importantes de agrosilvicultura. Nuestros resultados resaltan la necesidad de acciones regionales coordinadas para la conservación y restauración efectivas.【摘要】保护亚洲热带和亚热带森林的树木多样性是基于自然的解决方案的关注重点。物种面对多种威胁的脆弱性会影响生态系统服务的供给, 但目前人们对此仍知之甚少。我们对63种具有社会经济意义的树种面对过度开发、火灾、过度放牧、栖息地转换和气候变化的脆弱性进行了全区域空间显式评估。我们进行评估的树种来自国家优先保护名录, 树种的选择得到代表 20个国家的专家网络的认可。研究使用Maxent适宜性模型预测了物种分布范围, 并用可获取的空间数据集绘制了威胁暴露情况, 用功能特征估计了威胁敏感性。我们利用暴露地图和敏感性估计结果得到了物种特异的脆弱性地图, 并根据物种对当前威胁和气候变化的脆弱性, 确定了保护和恢复的优先区域。总的来说, 对这些树种的保护最重要的区域有 74%在保护区之外, 所有树种平均在其原生分布区 47%的范围内受到严重威胁。最紧迫的威胁是过度开发和栖息地转换;树木种群平均在其 24%和16%的范围内受到这些因素的严重威胁。我们的模型预测得到的气候变化整体影响有限, 尽管一些物种到2050年可能会因气候变化而失去超过15%的栖息地。我们发现婆罗洲雨林中特定的自然区域应作为森林遗传资源原地保护的热点地区, 该区域82%以上的面积位于已有保护区之外。我们还确定了西高止山、中南半岛旱林和苏门答腊雨林的退化地区为恢复热点地区, 在这些地区种植树木或辅助自然再生将有助于保护树种, 而印度南部和泰国的耕地则是潜在的重要农林地。我们的结果强调了为有效保护和恢复而采取区域性协调行动的必要性。【翻译: 胡怡思;审校: 聂永刚】.

Sengon (Falcataria moluccana) plantations, particularly in Java, have been facing threats from gall-rust disease caused by the pathogen Uromycladium falcatariae, which has caused considerable economic losses. Other species with morphological similarities with F. moluccana raises the question of whether the infected and non-infected sengon are the same species. This study aimed to confirm the haplotype of infected and non-infected sengon by gall-rust and compare them with a morphologically similar species of Albizia chinensis using the psbA-trnH intergenic spacer region molecular marker on chloroplast DNA. Wet wood samples from 32 infected plants and 32 non-infected plants were collected from Bogor and Ciamis, West Java, for analysis and comparison with A. chinensis sequences from the same gene region. The results showed that the infected and non-infected sengon came from the same haplotype with a genetic distance of 0 and InDel diversity of 0,031. Meanwhile, from 380 parallel aligned sites, there were 27 different sites between F. moluccana and A. chinensis. The genetic distance of the two species was classified as very low at 0.017. The psbA-trnH intergenic spacer region sequences of sengon in this study were the first to be uploaded to GenBank and can be accessed with accession numbers of LC456638.1 to LC456701.1. ABSTRAK Hutan tanaman sengon (Falcataria moluccana), khususnya di Pulau Jawa, pada umumya menghadapi serangan penyakit karat tumor yang disebabkan oleh patogen Uromycladium falcatariae, sehingga menimbulkan kerugian ekonomi yang cukup besar. Adanya spesies lain yang memiliki kemiripan morfologi dengan F. moluccana menimbulkan pertanyaan apakah sengon yang terserang dan tidak terserang karat tumor merupakan jenis yang sama. Penelitian ini bertujuan untuk mengonfirmasi jenis sengon yang terserang dan tidak terserang karat tumor dan membandingkan dengan spesies yang mirip secara morfologi, yaitu Albizia chinensis menggunakan penanda molekuler daerah psbA-trnH intergenic spacer pada DNA kloroplas. Sampel kayu basah dari 32 tanaman terserang dan 32 tanaman yang tidak terserang karat tumor diambil dari Bogor dan Ciamis, Jawa Barat, untuk dianalisis dan dibandingkan dengan sekuens A. chinensis dari wilayah gen yang sama. Hasil analisis menunjukkan bahwa sengon yang terserang dan tidak terserang berasal dari haplotipe yang sama dengan jarak genetik 0 dan keragaman InDel sebesar 0,031. Sementara itu, dari 380 situs yang sejajar, terdapat 27 situs yang berbeda antara F. moluccana dan A. chinensis. Jarak genetik kedua jenis tersebut tergolong sangat rendah yaitu 0,017. Sekuens daerah psbA-trnH intergenic spacer sengon dalam penelitian ini merupakan yang pertama diunggah di GenBank dan dapat diakses dengan nomer aksesi LC456638.1 sampai LC456701.1.

Several factors may compromise plant identifications provided by local informants, affecting the basis for incorporating local ecological knowledge (LEK) in scientific biodiversity assessments. Our study analyzes how site, informants’ background, and tree characteristics affect the consistency of folk identifications and the apparent correspondence between folk and Linnaean taxonomies. Twenty-eight informants performed in situ identifications of randomly selected trees at two sites in Indonesia and Vietnam. At both sites, older informants and informants who actively used trees had higher probabilities of proposing names for the trees. Naming consistency was higher where informants had good access to and daily use of the forest. Trees with particular morphological and anatomical characteristics, useful species, and landmark trees were more likely to be consistently named. Correspondences between folk and Linnaean taxonomies were clearer in the site with higher folk naming consistency. Findings contribute empirical evidence to inform research design and use of LEK in forest monitoring.

Molecular biodiversity surveys have been increasingly applied in hyperdiverse tropical regions as an efficient tool for rapid species assessment of partially undiscovered fauna and flora. This is done by overcoming shortfalls in knowledge or availability of reproductive structures during the sampling period, which often represents a bottleneck for accurate specimens’ identification. DNA sequencing technology is intensifying species discovery, and in combination with morphological identification, has been filling gaps in taxonomic knowledge and facilitating species inventories of tropical ecosystems. This study aimed to apply morphological taxonomy and DNA barcoding to assess the occurrence of Lamiaceae species in converted land-use systems (old-growth forest, jungle rubber, rubber, and oil palm) in Sumatra, Indonesia. In this species inventory, we detected 89 specimens of Lamiaceae from 18 species distributed in seven subfamilies from the Lamiaceae group. One third of the species identified in this study lacked sequences in the reference database for at least one of the markers used (matK, rbcL, and ITS). The three loci species-tree recovered a total of 12 out of the 18 species as monophyletic lineages and can be employed as a suitable approach for molecular species assignment in Lamiaceae. However, for taxa with a low level of interspecific genetic distance in the barcode regions used in this study, such as Vitex gamosepala Griff. and V. vestita Wall. ex Walp., or Callicarpa pentandra Roxb. and C. candidans (Burm.f.) Hochr., the use of traditional taxonomy remains indispensable. A change in species composition and decline in abundance is associated with an increase in land-use intensification at the family level (i.e., Lamiaceae), and this tendency might be constant across other plant families. For this reason, the maintenance of forest genetic resources needs to be considered for sustainable agricultural production, especially in hyperdiverse tropical regions. Additionally, with this change in species composition, accurate species identification throughout molecular assignments will become more important for conservation planning.

Arobaya AYS, Zuhud EAM, Siregar IZ, Irawati. 2022. Short Communication: Diversity and distribution of epiphytic orchid Dendrobium section Spatulata on the host plants in the Cycloop Mountain Nature Reserve of Papua, Indonesia. Biodiversitas 23: 2025-2034. Knowledge of species diversity and distribution is indispensable to assist in formulating a sound conservation strategy. This is truly significant for epiphytic orchids that grow on tree surfaces or phorophyte. The data and information are still limited despite their important role as biological indicators in forest ecosystems. The aim of the study was to carry out an inventory of epiphytic orchids and the phorophytes (host tress) in the Tropical Rain Forest of Cycloop Mountain Nature Reserve. The study took place in four sites located in the regency and city of Jayapura. Exploratory and sampling strip methods of 20x100 square meters were employed during the fieldwork. Orchids were observed and samples were collected based on their distribution in the host plants divided into five zones (Stem zone I and II; Crown zone III to V). The habitat's vegetation profiles were described using computer-aided design (CAD) verse 2018. Plant samples were identified in herbarium Manokwariense and the identified species were incorporated into CAD. Nineteen species of Dendrobium section Spatulata were identified as living as an epiphyte on the 12 phorophytes. These orchids and the host plants were described, determined and analyzed using PATN software. Most orchids are growing abundant in zone II and III. The phorophytes hosting the orchids in the lowland are mainly Syzygium and Intsia, while in the highland are Agathis and Podocarpus. Two types of vegetation profiles were described as savannah woodland in the low land area and coniferous trees vegetation in the highland above 650 m above sea level.

Rain tree (Samanea saman) is a dominant species commonly planted as a shade tree in urban landscapes in the tropics, including in Indonesia. This species is also targeted for routine inspection and monitoring within tree management systems. Management should particularly focus on tree health through assessment of tree growth conditions as well as tree stability. However, baseline information on the relationships of many key characteristics for the rain tree (e.g., growth, morphometrics, and biomechanics) is lacking. Therefore, an investigation was carried out to determine the relationship of morphometric parameters to tree growth, which included use of an acoustic tool to assess the elasticity of the tree stem. Fifty rain trees were selected for data collection and evaluated using stress wave velocity tools and visual morphometrics assessment. The results demonstrated that, in general, the trees had good vigor and stability. Statistical analysis showed a strong relationship between tree growth parameters and selected morphometric characteristics. The use of a nondestructive acoustic-based instrument enabled determining the modulus of elasticity, which provided equivalent results with the elasticity value obtained through a common method for determining tree stability. Only live crown ratio and tree slenderness parameters had a relationship with the dynamic modulus of elasticity. These results offer a means of measurement that is simpler, less expensive, and faster than current methods, although further testing is needed for different tree species and growth sites.

Shorea parvifolia (Dipterocarpaceae) is a widely distributed tree species which is important in terms of ecosystem functioning as well as forestry in Southeast Asia. During glacial periods, substantial precipitation decline is believed to have occurred in Southeast Asia, which considerably changed the distribution of the species. Repeated glacial and inter-glacial fluctuations were found to have influenced the genetic structure of the species, which is important to know for conservation and sustainable use. Leaf samples were collected from 18 populations covering most of the natural distribution of this species including the Malay Peninsula, Sumatra, and Borneo Islands. We investigated these samples using sequence data for eight chloroplast DNA (cpDNA) regions and 14 nuclear EST-SSR loci. The nucleotide diversity of cpDNA is higher in Malay Peninsula populations but the genetic diversity of nuclear DNA is higher in Borneo populations. The genetic structure revealed by nuclear DNA clearly separated Borneo populations from the rest, with an FST value of 0.150, while the genetic structure obtained from cpDNA was less pronounced (FST value = 0.136). Tajima’s D and Fu and Li’s D* for cpDNA showed statistical significance only in populations from Borneo. These results suggested that there has been recent population expansion of S. parvifolia in Borneo.

Mangifera casturi Kosterm., a mango plant from Kalimantan Selatan, Indonesia, has limited genetic information, severely limiting the research on its genetic variation and phylogeny. We collected M. casturi’s genomic information using next-generation sequencing, developed microsatellite markers and performed Sanger sequencing for DNA barcoding analysis. These markers were used to confirm parental origin and genetic diversity of M. casturi hybrids. The clean reads of the Kasturi accession were assembled de novo, producing 259 872 scaffolds (N50 = 1 445 bp). Fourteen polymorphic microsatellite markers were developed from 11 040 microsatellite motif-containing sequences. In total, 58 alleles were produced with a mean of 4.14 alleles per locus. Microsatellite marker analysis revealed broad genetic variation in M. casturi. Phylogenetic analysis was performed using internal transcribed spacers (ITS), matK, rbcL, and trnH-psbA. The phylogenetic tree of chloroplast markers placed Kasturi, Cuban, Pelipisan, Pinari, and Hambawang in one group, with M. indica as the female ancestor. Meanwhile, the phylogenetic tree of ITS markers indicated several Mangifera species as ancestors of M. casturi. Thus, M. casturi very likely originated from the cross-hybridization of multiple ancestors. Furthermore, crossing the F1 hybrids of M. indica and M. quadrifida with other Mangifera spp. may have generated much genetic variation. The genetic information for M. casturi will be a resource for breeding improvement, and conservation studies.

Shorea is a genus of the Dipterocarpaceae family which shows high economic and ecological values. Currently, market demand for wood is still met from natural forests that are degrading due to over exploitation. The degraded forests are included in the rehabilitation program that require significant number of planting stocks, mainly from seedlings or wildlings. Correct species identification of planting stocks, particularly wildlings, is an important first step in planting activities and it is relevant with Shorea spp planting stocks due to leaf morphology traits. This study was aimed to examine the traits of leaf morphology of four Shorea species for easy field identification. Samples were taken from five trees for each species. Morphological trait identification was conducted by leaf observation and measurement. The results showed that all four species had the same apex shape. Six of the twelve morphological characteristic of the variable character can influence the morphological characteristics of the leaves, namely the roving leaf, leaf area, leaf length, aspect ratio, form factorand perimeter ratio of diameter. S. leprosula is similar to S. parvifolia, while S. ovalis is similar to S. guiso. Shorea leaf character S. parvifolia differentiated from S. leprosula, while Shorea ovalis similar to Shorea guiso. The LP variable (length from the base of the leaf to the widest point of the leaf) is a variable that can be used as aleaf morphological characteristic of the four Shorea which are compared because each one has a different value. S. ovalis has the highest LP value, followed by S. guiso and S. leprosula, while S. parvifolia has the lowest LP value. Keywords: apex shape, identification, leaf morphology traits, Shorea

Background: Climate plays an important role in the growth process of various plant species, and the ebony species is no exception to this. Ebony (Diospyros celebica Bakh.) is a Sulawesi endemic flora species whose wood is widely used as a material for light to heavy construction and important raw material for Indonesia’s timber industry. Massive forest exploitation threatens preservation of the Diospyros celebica, so conservation is needed to save it from extinction. However, the difficulty in distinguishing Diospyros celebica in the tillering phase caused many seed collection errors, so a system was needed to identify plants correctly. This study aims to extract leaf morphological features, analyze the correlation between leaf morphological features and climate variables, and classify them based on the region where Diospyros celebica grows. Result: The results show that several leaf morphological characteristics were sufficiently correlated with climate variables such as MAT and MAP. In dry weather, the leaves of Diospyros celebica tend to have relatively small leaf sizes, whereas, in wetter weather, the leaves tend to have a much larger size. The classification results could distinguish leaf morphology based on growing regions with an accuracy rate of 94.59%. Conclusions: By obtaining a high level of classification accuracy, it can be interpreted that the size of the leaf morphology of the same species (Diospyros celebica) is different in each region, influenced by climate variables, in this case MAT and MAP.

Nugroho A, Matra DD, Siregar IZ, Haneda NF, Istikorini Y, Rahmawati R, Amin Y, Siregar UJ. 2021. Early growth evaluation and estimation of heritability in a sengon (Falcataria moluccana) progeny testing at Kediri, East Java, Indonesia. Biodiversitas 22: 2728-2736. Sengon (Falcataria moluccana) is widely cultivated as a monoculture plantation in community forests that still poses a high risk of pest and disease attacks. Although plants naturally show signs of resistance, there is still however a lack of understanding on the influence of relative plant resistance on growth due to environmental and genetic factors. This study was aimed to evaluate the early growth of a sengon progeny testing in Kediri, East Java and to estimate the genetic parameters with respect to its resistance and susceptibility to stem borer and rust disease. Progeny test was designed using randomized complete block design (RCBD) with four blocks as replications. Each block consists of 100 families which was grouped into two categories, 50 families for resistant group and 50 families for susceptible group. Observations were made at the age of 0 and 9 months after planting on 9 individuals per plot in each family. Growth characteristics were measured as germination (%), height (m), and diameter (cm). Results indicated that the sengon growth rate from resistant and susceptible plant groups showed a significant difference. Heritability values are classified as moderate, ranging between 0.107 to 0.133 and 0.102 to 0.150 for height and diameter variables, respectively. These information may be used for gradual selection by considering the appropriate intensity.

Background: Mangifera casturi Kosterm. is an endemic local mango fruit from Kalimantan Selatan, Indonesia. The limited genetic information available on this fruit has severely limited the scope of research into its genetic variation and phylogeny. This study aimed to collect genomic information from M. casturi using next-generation sequencing technology and to develop microsatellite markers and perform Sanger sequencing for DNA barcoding analysis.Results: The clean reads of the Kasturi accession of M. casturi were assembled de novo using a Ray assembler, producing 259,872 scaffolds with an N50 value of 1,445 bp. Fourteen polymorphic microsatellite markers were developed from 11,040 sequences containing microsatellite motifs. In total, 55 alleles were produced, and the mean number of alleles per locus was 3.93. Results from the microsatellite marker analysis revealed broad genetic variation in M. casturi. Phylogenetic analysis was performed using internal transcribed spacers (ITS), matK, rbcL, and trnH-psbA. The phylogenetic tree of chloroplast markers showed that Kasturi, Mawar, Pelipisan, Pinari, and Hambawang belong to one group, with M. indica as the female ancestor. In comparison, the phylogenetic tree of ITS markers indicated several Mangifera species as multiple ancestors of M. casturi. Conclusions: This study strongly suggested that M. casturi originated from the cross-hybridization of multiple ancestors. Further, crossing the F1 hybrids of M. indica and M. quadrifida with other Mangifera spp. was hypothesized to produce the observed high genetic variation. The genetic information for this fruit is also a resource for the breeding and improvement as well as for conservation studies of this species.

Objectives Development of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. To the best of our knowledge, there has been no published reports on the use of ONT for genomic analysis of Indonesian tree species. This progress is promising for further improvement in order to acquire more genomic data for research purposes. Therefore, the present study was carried out to determine the effectiveness of ONT in generating long-read DNA sequences using DNA isolated from leaves and wood cores of Macassar ebony (Diospyros celebica Bakh.). Data description Long-read sequences data of leaves and wood cores of Macassar ebony were generated by using the MinION device and MinKnow v3.6.5 (ONT). The obtained data, as the first long-read sequence dataset for Macassar ebony, is of great importance to conserve the genetic diversity, understanding the molecular mechanism, and sustainable use of plant genetic resources for downstream applications.

Ebony (Diospyros celebica Bakh.) is an endemic plant on Celebes (Sulawesi) island. Extractive compounds within ebony wood cause it to have durability, strength, and beautiful patterns. In this study, we used near-infrared (NIR) spectroscopy to discriminate between ebony wood samples, based on their origins at different growth sites on Celebes island, and to develop quantitative models to predict the extractive content of ebony wood. A total of 45 wood meal samples from 11 sites located in West, Central, and South Celebes were collected in this study. NIR spectral data were acquired from hot water and ethanol&ndash, benzene soluble extracts from ebony wood in this study. The extractive content of the ebony was 10.408% and 10.774% based on hot water solubility and treatment with ethanol&ndash, benzene solvent, respectively. Multivariate analysis based on principal component analysis&ndash, discriminant analysis revealed that ebony wood from West Celebes differed from most of the wood from South Celebes, however, it was only slightly different from ebony wood from Central Celebes based on NIR spectra data. These findings were in line with the extractive contents obtained. Partial least square regression models based on wood meal spectra could potentially be used to estimate the hot water and ethanol&ndash, benzene extractive contents from ebony wood.

Siregar IZ, Muharam KF, Purwanto YA, Sudrajat DJ. 2020. Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming. Biodiversitas 21: 4558-4564. Gmelina (Gmelina arborea) seed collected from tropical Indonesian forest experience seed deterioration during storage which is relatively faster than the gmelina seeds originating from temperate regions, such as India and Myanmar. Various treatments have been made to improve the seed viability and vigor after storage through various invigoration techniques. However, the utilization of promising novel technology such as ultrafine bubbles (UFB) has not been evaluated yet. The objective of this study was to determine the effect of seed priming using UFB, polyethylene glycol (PEG), and gibberellic acid (GA3) on the viability and vigor of gmelina seeds that have been stored for one and two years. This study was conducted by employing Completely Randomized Design (CRD) using three replicates. The study showed priming treatment had significant effect and formed average germination capacity of 80% for the seed stored for 1 year. On the other hand, the priming treatment using PEG-0.8 MPa was the best treatment for germination capacity of 74.67% on 2 years stored seeds. UFB had significant effect on germination capacity, germination rate, growth uniformity, and germination value. This result was expected due to reactive oxygen species (ROS) produced by micro-nano bubbles that could increase physiological activity of the seed cells.

Mistletoes are hemiparasitic macroparasite plants which interfere with trees and other wild plants in nutrient acquisition. As the plant has low leaf water potential, it draws water from teak wood tissues during the deciduous stage of the teak host, thereby killing the twigs and eventually, the teak tree. Mistletoes are also a key player in plant diversity. Therefore, the mistletoe population needs to be regulated not only as a parasite but also as a keystone species affecting biodiversity. Knowledge scarcity on the status of mistletoes includes its genetic variation. Hence, the purpose of this study is to analyze the level of genetic variation of teak mistletoe (Dendrophthoe pentandra) using RAPD marker. At Padangan teak Clonal Seed Orchard (CSO), it was randomly collected leaf samples from three layers of the mistletoe’s crown (upper, middle, and below) were taken from five host teak trees randomly selected from each of the sub-observation measure plots (OMP). Four OMP units inside the observation sample plots (OSP) (n = 3, 50 x 50 m) at different levels of infestation (light, moderate and heavy) were established. Analysis of the genetic variation and genetic distance of mistletoes hanging on the different crown layers were conducted using RAPD markers. The leaf samples from the crown layers, UU (upper crown and sub-section upper), UM (upper crown and sub-section middle), and UB (upper crown and sub-section below), which include U (upper crown) had significantly greater genetic variation (He = 0.181 – 0.255) than those from M layer (middle crown, He = 0.227) and the B layer (below crown, He = 0.114). Furthermore, the widest genetic distance significantly occurred between the mistletoes of the UB and B crown layers (0.310), whereas the nearest genetic distance significantly occurred between mistletoes of UU and UM layers in the upper crown (0.038). Practical implications of the low genetic variation in this study include the control of mistletoe D. pentandra infestation by means of restricting its population so that Perhutani State Owned Forestry Enterprise can maintain the level of damage below the economic threshold.

Objectives Efforts to restore tropical peat swamp forests in Indonesia face huge challenges of potential failures due to socio-economic factors and ecological dynamics attributed to lack of knowledge on the adaptive mechanisms of potential tree species such as Kapur (Dryobalanops aromatica C.F.Gaertn Syn. Dryobalanops sumatrensis J.F. Gmelin A.J.G.H Kostermans). This species is a multi-purpose tree that, commonly grows in mineral soils, but also in peat swamp as previously reported, which raised a fundamental question regarding the molecular mechanism of this adaptation. Therefore, a dataset was created aiming to detect candidates of adaptive genes in D. aromatica seedlings, cultivated in two contrasting potting media, namely mineral soil and peat media, based on RNA Sequencing Transcriptome Analysis. Data description The RNA transcriptome data of D. aromatica’s seedlings derived from young leaves of three one-year-old seedlings, raised in each dry mineral soil media and peat media, were generated by using Illumina HiSeq 4000 platform in NovogenAIT, Singapore. The acquired data, as the first transcriptome dataset for D. aromatica, is of a great importance in understanding molecular mechanism and responses of the involved genes of D. aromatica to the contrasting, growing potting media conditions that could also be useful to generate molecular markers.

Ariawan I, Herdiyeni Y, Siregar IZ. 2020. Short Communication: Geometric morphometric analysis of leaves venation in Shorea spp. for identification using Digital Image Processing. Biodiversitas 21: 3303-3309. Shorea is one of the genera of the Dipterocarpaceae family which consists of more than 190 species. Massive exploitation of forests has threatened the sustainability of Shorea in nature. A total of 156 species has been listed on the IUCN (International Union for Conservation of Nature) red list. From the 156 species, 59.6% are in the critically endangered category, so urgent conservation is needed. However, during collection of Shorea at the seedling phase for conservation purposes, it is often difficult to distinguish among them that can cause errors in their collection process. To avoid these errors, identification needs to be done, usually based on plant leaf and flower morphology. Leaves are easier because they have the main features that distinguish each plant species, one of which is the venation structure. Geometric morphometric techniques are a modern approach recognized as useful for the identification of species in many plants. Geometric morphometrics analyzes the position of the venation point using coordinate geometry values. This research was aimed to extract venation features of Shorea leaves using a geometric morphometric approach. The extraction process result in some features, such as straightness, different angle, length ratio, scale projection, and secondary nerves. On extracted features, an analysis was then performed to find out the best features in classifying species of Shorea spp. The results of this study indicated that the geometric morphometric approach could extract the value of the features of straightness, different angle, length ratio, scale projection, and secondary nerves. The secondary nerve feature is the best feature because it can distinguish between fourcommonly planted species of Shorea spp. (S. acuminata, S. leprosula, S. ovalis, and S. selanica). By using the support vector machine classification technique to identify species of Shorea spp., the classification results obtained an average accuracy of 84.46%.

Indriani F, Siregar U, Matra DD, Siregar IZ. 2019. Ecological aspects and genetic diversity of Shorea balangeran in two forest types of Muara Kendawang Nature Reserve, West Kalimantan, Indonesia. Biodiversitas 20: 482-488. Shorea balangeran (balangeran) belongs to the Dipterocarpaceae family that is distributed naturally in Indonesia with a wide range of ecological conditions including peat swamp and heath forests. Good adaptability of S. balangeran remains unknown concerning molecular (genetic) responses that are important to provide scientific knowledge for supporting forest restoration, especially for peatland restoration efforts. The main objective of this research was to analyze the ecological aspects and genetic diversity of S. balangeran in two types of forests, namely: peat forests (wetland) and heath forests (dry land). Field research was conducted in Muara Kendawangan Nature Reserve, West Kalimantan following standard vegetation analysis by establishing purposively sample plots (50 m x 50 m). Genetic diversity was assessed by RAPD analysis using ten random polymorphic primers. Results showed that both in heath and peat forests, S. balangeran was dominant, but only at pole and tree stages indicating that poor regeneration capacity of S. balangeran. This finding also confirmed the critically endangered status of S. balangeran as listed in the IUCN Red List of Threatened Species. Genetic analysis using RAPD did not show a clustering of S. balangeran between peat forest and heath forest populations, indicating that S. balangeran has good adaptability to grow in both forest types.

Teak (Tectona grandis Linn. f.), one of the most-valued tree species in the world, is slow-growing with a long period until it can be harvested; therefore, ensuring that only high-quality seedlings or clones are selected for planting is critical. The main objective was to determine performance and repeatability of selected clones in terms of growth and survival rates in different micro-environments. A 2-year clonal trial using 41 clones and a local seedling of teak as a control were grown at 4 microsites differing in spacing, soil fertility and alley crops to assess tree height, diameter and survival rate that was evaluated in Purwakarta, West Java, Indonesia using a randomized complete block design with four replicates of each clone at each microsite. Teak growth was influenced by clone (p

Melia azedarach L. is one of the fast growing spesies which is potential to be develoved in community forest. This species is found to ocuppy most agroforestry lands in Selaawi Village (Garut, West Java). Based on interview with community that there are two types small fruit mindi and big fruit mindi. Microsatelit marker were used to assess the genetic variation. The results showed that the genetic variation of mindi in Selaawi Village was high ranging from He 0,379 – 0,439.

Arianto W, Zuhud EAM, Hikmat A, Sunarminto T, Siregar IZ. 2018. Genetic diversity of Amorphophallus titanum in Bengkulu, Indonesia based on RAPD markers. Biodiversitas 19: 1783-1790. Titan Arum [Amorphophallus titanum (Becc.) Becc. Ex Arcang], a plant species belonging to the family of Araceae is known for its gigantic floral size and elicited rotten fragrance when the flower bloom. Since it remains only found in Sumatran island, many authors categorized the plant as endemic species. The population of the species in the natural habitat has significantly declined because of the conversion of forest land mainly into plantations or other land uses. Considering the importance of conservation attempts to A. titanum, a sufficient data on genetic diversity of the species is necessary. The research was aimed to determine the genetic diversity within and among populations of A. titanum in some area of protected forests in Bengkulu Province, comprising the population of Palak Siring, Tebat Monok, and Air Selimang. RAPD genetic DNA fingerprinting approach was used to assess the genetic diversity of A. titanum using 13 preselected DNA primer: OPA 11, OPA 19, OPC 04, OPN 14, OPN 19, OPU 03, OPU 06, OPU 07, OPB 17, OPC 07, OPO 04, OPU03-1, OPNI 18E. The result revealed that the method has successfully produced several DNA fragments with varied length ranging from 250 bp to 2000 bp with 4-16 variation in polymorphic bands. Based on RAPD marker analysis, the population of Air Selimang was considered as a potential center of diversity of A. titanum because of the others two populations had a lower genetic diversity. In general, the genetic diversity among populations was lower than within population. The cluster analysis of the genetic similarity of 22 individuals of the three populations resulted in the separation into two main groups with the first group consisting of 17 individuals (Population Air Selimang and Tebat Monok) and the second group of 5 individuals (Palak Siring population).

Large-scale genetic structure revealed in tree populations in SE Asia, as well as in many temperate forests, has been shaped by climatic fluctuation in the late Pleistocene, most importantly by that in the last glacial period. In a comparative study of the phylogeographic patterns of two closely related dipterocarp species, Dryobalanops aromatica C.F.Gaertn. and D. beccarii Dyer, we investigated how changes in land area associated with changes in climate affected large-scale genetic structure. We examined the genetic variation of D. aromatica, collected from nine populations throughout the Sundaic region, and of D. beccarii, collected from 16 populations mainly in Borneo, using seven polymorphic microsatellite markers. The two species were clearly distinguishable in the STRUCTURE analysis, although hybridisation probably occurred in sympatric populations and also in several other populations. The D. aromatica populations were divided into two main groups by the STRUCTURE analysis: Malay–Sumatra and Borneo. Mixing of the Sumatra and Borneo clusters occurred on the Malay Peninsula, supporting the hypothesis that tropical rainforests expanded over a dried Sunda Shelf during the last glacial period. The two main genetic clusters might have been formed by repeated cycles of fluctuation in land area. The D. beccarii populations in Borneo were divided into four geographically distinct groups: western Sarawak, central inland Sarawak, central coastal Sarawak and Sabah. The population on the Malay Peninsula (Gunung Panti) was an admixture of the four Bornean clusters. This suggests that this population is a relic of the recent range expansion of D. beccarii during the last glacial period.

Until now, gall rust disease is a major threat for Falcataria moluccana plantations in Indonesia. One of the environmental factors that can influence the development of plant diseases is the cultivation practice. However, the studies related to the influence of cultivation practice to the F. moluccana gall rust disease are still limited. This study aimed to determine the correlation between cultivation practice to the incidence and severity of F. moluccana gall rust disease. The incidence and severity of F. moluccana gall rust disease were observed from 47 planting sites distributed throughout Java. Its correlation to the cultivation practice was analyzed using chi square analysis followed by coordinate analysis. The results indicated that 6 of 13 variables were significantly correlated with disease incidence. Meanwhile, only 3 of 13 variables were significantly correlated to disease severity. These factors were plant age, use of organic fertilizer, and chemical control. Based on coordinate analysis result, organic fertilizer and chemical control application showed strong association with low level disease severity. Keywords: Chemical control, Java, organic fertilizer, plant age ABSTRAK Penyakit karat puru masih menjadi permasalahan yang utama pada tanaman sengon di Indonesia. Tanaman sengon dapat diserang pada semua tingkatan umur dan telah menimbulkan kerugian yang signifikan. Salah satu faktor lingkungan yang memengaruhi perkembangan penyakit tanaman ialah faktor budidaya. Penelitian ini bertujuan untuk mengetahui hubungan antara faktor budidaya dengan insidensi dan keparahan penyakit karat puru pada sengon. Sebanyak 47 lokasi penanaman sengon yang tersebar di seluruh Jawa diamati insidensi dan keparahan penyakitnya. Hubungannya dengan faktor budidaya dianalisis menggunakan Khikuadrat yang dilanjutkan dengan analisis korespondensi. Hasil penelitian menunjukkan sebanyak 6 dari 13variabel faktor budi daya menunjukkan korelasi yang signifikan terhadap insidensi penyakit. Sementara itu terhadap keparahan penyakit, sebanyak 3 dari 13 variabel faktor budidaya menunjukkan korelasi yangsignifikan. Ketiga faktor tersebut yaitu umur tanaman, penggunaan pupuk organik, dan pengendalian kimiawi. Hasil analisis korespondensi menunjukkan faktor penggunaan pupuk organik dan pengendalian secara kimiawi berasosiasi dengan keparahan penyakit yang rendah. Kata kunci: Jawa, pengendalian kimiawi, pupuk organik, umur tanaman

Sudrajat DJ, Suwandhi I, Siregar IZ, Siregar UJ. 2018. Variation in seed morpho-physiological and biochemical traits of Java olive populations originated from Java, Bali, Lombok, and Timor Islands, Indonesia. Biodiversitas 19: 1004-1012. Java olive (Sterculia foetida L.) is one of the promising non-edible feedstocks for biodiesel production. Utilization of this tree species for plantation is limited due to knowledge gaps in many fundamental aspects of seed properties. The aim of the study was to determine the variation that may exist among java olive seed collected from Bogor, Palimanan, Tuban, Gilimanuk, Pemenang and Naioni populations, and to analyze correlations between one or many seed morpho-physiological and biochemical traits. Analysis of variance showed significant differences of seed morphological and biochemical traits, except for the germination capacity. Seed morphological traits were not significantly correlated with agro-climate factors, while some of biochemical traits revealed significant correlation with some agro-climate factors. Genotypic variance and genotypic coefficient of variation were higher for fruit length, fruit width, carbohydrate, fat, protein and oil contents, indicating higher genetic contribution on the variance of these traits. High heritability values combined with high genetic gain was found for protein content, fruit width and oil content, that revealed the characters having highly genetic origin with a good number of heritable components. Population clustering exhibited that most of the geographically distant population are genetically related. Pemenang population was found highly associated with oil content, indicating that the population have high potency to develop as the feedstocks for for biodiesel production.

REDD+ aims to reduce emissions from deforestation and forest degradation, conservation, enhancement of forest carbon stocks, and sustainable forest management (SFM). An assessment of agroforest is important for REDD+ implementation in Indonesia. In this context, evaluation of the existing agroforests and their uses is an important issue. The objectives of this research were to characterize agroforest diversity with respect to habitat, regulation, production and information functions in Kampung Birang and Kampung Merabu, Berau, East Kalimantan. This research focused on two size classes of trees, namely medium trees (10≤dbh≤20 cm) and large trees (dbh>20 cm). Local community members were trained in plot establishment (60 plots, 50x50 m), tree diameter measurement, trees tagging, and herbarium collection. Local community members identified tree species by local names and listed the species uses. Results showed that agroforests as habitat obtained diversity indices (H’) of moderate category for Kampung Birang and high category for Kampung Merabu. Otherwise, Margalef’s index (Dmg) for the medium and large tree classes in Kampung Birang and Kampung Merabu were classified as high species richness category. This finding implies that enrichment planting using high economic value species is still necessary in Kampung Birang. Kampung Merabu was found to be more diverse than Kampung Birang, although soil quality in Kampung Birang was better than that in Kampung Merabu. Disturbances due to human activities that potentially impact agroforest stability were also pre-identified. The top ten tree species were classified based on importance value index (IVI) across both villages consisting of 80% shade intolerant and 20% semi-tolerant species. Agroforest showing production and information function as produces various edible fruits that are considered as key component for maintaining biodiversity sustainability and ecosystem functioning. REDD+ aims to reduce emissions from deforestation and forest degradation, conservation, enhancement of forest carbon stocks, and sustainable forest management (SFM). An assessment of agroforest is important for REDD+ implementation in Indonesia. In this context, evaluation of the existing agroforests and their uses is an important issue. The objectives of this research were to characterize agroforest diversity with respect to habitat, regulation, production and information functions in Kampung Birang and Kampung Merabu, Berau, East Kalimantan. This research focused on two size classes of trees, namely medium trees (10≤dbh≤20 cm) and large trees (dbh>20 cm). Local community members were trained in plot establishment (60 plots, 50x50 m), tree diameter measurement, trees tagging, and herbarium collection. Local community members identified tree species by local names and listed the species uses. Results showed that agroforests as habitat obtained diversity indices (H’) of moderate category for Kampung Birang and high category for Kampung Merabu. Otherwise, Margalef’s index (Dmg) for the medium and large tree classes in Kampung Birang and Kampung Merabu were classified as high species richness category. This finding implies that enrichment planting using high economic value species is still necessary in Kampung Birang. Kampung Merabu was found to be more diverse than Kampung Birang, although soil quality in Kampung Birang was better than that in Kampung Merabu. Disturbances due to human activities that potentially impact agroforest stability were also pre-identified. The top ten tree species were classified based on importance value index (IVI) across both villages consisting of 80% shade intolerant and 20% semi-tolerant species. Agroforest showing production and information function as produces various edible fruits that are considered as key component for maintaining biodiversity sustainability and ecosystem functioning.

Pamoengkas P, Siregar IZ, Dwisutono AN. 2018. Stand structure and species composition of merbau in logged-over forest in Papua, Indonesia. Biodiversitas 19: 163-171. Single selective cutting is the most common form of timber extraction in natural forest in the tropics. Although, vast tracts natural forests are already logged-over, the effect of logging on the structure and species composition has been sparsely documented, especially for merbau (Intsia bijuga). Merbau is an excellent timber species and intensively harvested in Papua. The objective of this study was to analyze the structure and species composition of merbau in the several logged-over forests at different ages, i.e., 1, 5, 11, 16, 21 years old after selective logging and primary forest as the reference plot. The study was conducted in February 2016 at Sarmi District, Papua. The distributions of merbau in each plot varies but when they were compared with that in the primary forest that had reached 9.81%. Thus, the decrease of merbau trees in the plots of logged forest areas was not significant. Index of Diversity of all growth stages was high. In general, the number of merbau seedling regeneration in the study plots are above the average value standardized in Selective Cutting and Replanting System (TPTI) regulation. With regard to the condition of sapling regeneration, we concluded that selective logging might not provide enough growing space for regeneration of merbau. Providing appropriate growing space is one of the key factors to achieve adequate merbau natural regeneration.