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14,070 results on '"Instituto de Biologia"'

1. Archivos do Instituto de Biologia Vegetal

Author: Instituto de Biologia Vegetal, BHL SciELO, and Instituto de Biologia Vegetal
Published: 1990

2. A saúde não é assim tão única: ressignificando discursos sobre (re)emergências de zoonoses

Author: Rodrigues, Claudio Manuel, Fundação Oswaldo Cruz - Presidência - CDTS, Geise, Lena, UERJ - Instituto de Biologia - Departamento de Zoologia, Gazeta, Gilberto Salles, Fundação Oswaldo Cruz - IOC - LIRN, De Oliveira, Stefan Vilges, Universidade Federal de Uberlândia - Faculdade de Medicina - Departamento de Saúde Coletiva, and Não há financiamento para pesquisa e produção desse manuscrito.
Subjects: Ecologiam Médica, EcoHealth, opinions, opiniones, environmental health, Saúde Pública, Epidemiologia, discurso, zoonosis, medical ecology, zoonoses, Salud ambiental, One health, Ecosalud, Ecología médica, Saúde Ambiental, One Health, Ecohealth
Abstract: A Ecologia Médica estuda a adaptação do ser humano ao ambiente em que vive, observando os reflexos sobre sua saúde e as interações desse com o ecossistema. One Health é uma estratégia global de expansão das colaborações interdisciplinares e de comunicação entre grupos de várias nações beneficiando os cuidados de saúde, em seus aspectos mais amplos, para seres humanos, animais e o meio ambiente. Ecohealth é um movimento que busca a saúde sustentável para seres vivos e ecossistemas, promovendo a compreensão dessa complexidade através de metodologias que encorajem a resolução de problemas pela reunião de múltiplos conhecimentos oriundos das ciências sociais, naturais e da saúde. O objetivo desse artigo é descrever possíveis desafios para os que militem na Saúde Ambiental analisando similaridades e discrepâncias dos diferentes discursos quanto à emergência e reemergência de antropozoonoses por meio de uma revisão narrativa da literatura. La Ecología Médica estudia la adaptación del ser humano al medio en el que vive, analizando los efectos sobre su salud y sus interacciones con el ecosistema. One Health es una estrategia global que apuesta por la colaboración multidisciplinar y la comunicación entre grupos de diversos países, teniendo como meta la salud, no solo la de los seres humanos, sino también la de los animales y la del medio ambiente. Por su parte, Ecosalud es un movimiento que busca la salud sostenible, tanto de los seres vivos, como de los ecosistemas. Entender esta compleja relación no resulta fácil; para ello, promueve estudios en los que aplica metodologías que incentivan la resolución de los problemas utilizando los conocimientos provenientes de las ciencias sociales, naturales y de la salud. El objetivo de este artículo es describir posibles retos para la Salud Ambiental, analizando similitudes y discrepancias en diferentes discursos sobre el surgimiento y resurgimiento de la antropozoonosis, a través de una revisión de la literatura científica. Medical Ecology studies how human beings adapt to their environment, analyzing the effects on their health and their interactions with ecosystems. One Health is a global strategy that relies on multidisciplinary collaboration and communication among groups from different countries with the aim of promoting health, not only of humans, but also of animals and the environment. On the other hand, EcoHealth is a movement that strives for sustainable health—both of living beings and ecosystems. Understanding this complex relationship is not easy. To this end, EcoHealth promotes studies using methodologies that encourage problem-solving using knowledge drawn from the social, natural and health sciences. The aim of this article is to describe the potential challenges Environmental Health faces and study the similarities and discrepancies in the different discourses on the emergence and re-emergence of anthropozoonoses by reviewing scientific literature.
Published: 2021

3. Categorization of plant uses utilized by the inhabitants of urban and rural areas of Peru

Author: Albán-Castillo, Joaquina, Departamento de Etnobotánica y Botánica Económica. Museo de Historia Natural. Universidad Nacional Mayor de San Marcos. Apartado 14-0434, Lima 14, Chilquillo Torres, Eder, Departamento de Etnobotánica y Botánica Económica. Museo de Historia Natural. Universidad Nacional Mayor de San Marcos. Apartado 14-0434, Lima 14, PERÚ. Programa de Pós-Graduação em Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, BRAZIL, Melchor-Castro, Briggitthe, Departamento de Etnobotánica y Botánica Económica. Museo de Historia Natural. Universidad Nacional Mayor de San Marcos. Apartado 14-0434, Lima 14, PERÚ. Institute of Molecular Plant Sciences. School of Biological Sciences, The University of Edinburgh, Edinburgh, UNITED KINGDOM Tropical Diversity Section, Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh, UNITED KINGDOM, Cochachin Guerrero, Elizabeth, Castillo Vera, Hellen, Hurtado-Huarcaya, José, Departamento de Etnobotánica y Botánica Económica. Museo de Historia Natural. Universidad Nacional Mayor de San Marcos. Apartado 14-0434, Lima 14, PERÚ Departamento de Biología. Facultad de Ciencias. Universidad Nacional Agraria La Molina. Av. Universidad S/N, La Molina, Lima., and Cruz-Ríos, Isabeu
Subjects: Use of plant resources, categorization, traditional knowledge, Peru, uso de recursos vegetal, categorización, conocimiento tradicional, Perú
Abstract: En la actualidad el uso y aprovechamiento de los recursos vegetales ha derivado en numerosas investigaciones etnobotánicas, las cuales vienen documentando una rápida erosión de conocimientos tradicionales en las culturas nativas peruanas, incentivando su preservación y consecuente revalorización. Con el fin de facilitar el análisis de la data obtenida en los múltiples estudios realizados, esta investigación constituye una aproximación de los criterios de clasificación de las categorías de uso de las especies vegetales asociadas a la vida y cultura del poblador peruano. La definición de las categorías de uso presentes en este documento constituye el resultado de 130 expediciones de campo realizadas por los autores en comunidades costeras, andinas y amazónicas peruanas, así como en la evaluación de 17 281 registros de usos documentados en nueve herbarios peruanos y extranjeros. Se proponen nueve categorías de usos, subdivididas en 60 subcategorías: medicinal (21), social (8), alimento humano (3), alimento para animales (3), materiales (10), tóxicas (4), etnoveterinario (2), ambiental (6) y combustible (3). Se concluye que la propuesta de una estandarización de categorías de uso facilitará la compilación y análisis del uso de plantas dentro de una comunidad, y a la vez permitirá realizar comparaciones entre ecosistemas y/o comunidades. Nowadays, the use and harnessing of vegetal resources has resulted in much ethnobotanical research, which has documented a rapid erosion of traditional knowledge in native Peruvian cultures, encouraging its preservation and consequent revaluation. In order to ease the analysis of the data obtained in the multiple studies that have been made, this proposal constitutes an approximation of the classification criteria for the categories of plant uses associated with the life and culture of Peruvian inhabitants. These use categories’ definitions are the result of 130 field expeditions carried out by the authors, in coastal, Andean and Amazon Peruvian communities, as well as the evaluation of 17 281-use registries documented in nine foreign and Peruvian herbaria. The nine use categories proposed in this article, are divided in 60 subcategories: medicinal (21), social (8), human food (3), animal food (3), materials (10), toxic (4), ethnoveterinary medicine (2), environmental (6) and fuel (3). The authors conclude that the proposal of the use categories standardization will simplify the compilation and analysis of plant uses in a community, and it will allow making comparisons between different ecosystems and communities.
Published: 2021

4. A saúde não é assim tão única: ressignificando discursos sobre (re)emergências de zoonoses

Author: Não há financiamento para pesquisa e produção desse manuscrito., Rodrigues, Claudio Manuel; Fundação Oswaldo Cruz - Presidência - CDTS, Geise, Lena; UERJ - Instituto de Biologia - Departamento de Zoologia, Gazeta, Gilberto Salles; Fundação Oswaldo Cruz - IOC - LIRN, De Oliveira, Stefan Vilges; Universidade Federal de Uberlândia - Faculdade de Medicina - Departamento de Saúde Coletiva, Não há financiamento para pesquisa e produção desse manuscrito., Rodrigues, Claudio Manuel; Fundação Oswaldo Cruz - Presidência - CDTS, Geise, Lena; UERJ - Instituto de Biologia - Departamento de Zoologia, Gazeta, Gilberto Salles; Fundação Oswaldo Cruz - IOC - LIRN, and De Oliveira, Stefan Vilges; Universidade Federal de Uberlândia - Faculdade de Medicina - Departamento de Saúde Coletiva
Abstract: A Ecologia Médica estuda a adaptação do ser humano ao ambiente em que vive, observando os reflexos sobre sua saúde e as interações desse com o ecossistema. One Health é uma estratégia global de expansão das colaborações interdisciplinares e de comunicação entre grupos de várias nações beneficiando os cuidados de saúde, em seus aspectos mais amplos, para seres humanos, animais e o meio ambiente. Ecohealth é um movimento que busca a saúde sustentável para seres vivos e ecossistemas, promovendo a compreensão dessa complexidade através de metodologias que encorajem a resolução de problemas pela reunião de múltiplos conhecimentos oriundos das ciências sociais, naturais e da saúde. O objetivo desse artigo é descrever possíveis desafios para os que militem na Saúde Ambiental analisando similaridades e discrepâncias dos diferentes discursos quanto à emergência e reemergência de antropozoonoses por meio de uma revisão narrativa da literatura., La Ecología Médica estudia la adaptación del ser humano al medio en el que vive, analizando los efectos sobre su salud y sus interacciones con el ecosistema. One Health es una estrategia global que apuesta por la colaboración multidisciplinar y la comunicación entre grupos de diversos países, teniendo como meta la salud, no solo la de los seres humanos, sino también la de los animales y la del medio ambiente. Por su parte, Ecosalud es un movimiento que busca la salud sostenible, tanto de los seres vivos, como de los ecosistemas. Entender esta compleja relación no resulta fácil; para ello, promueve estudios en los que aplica metodologías que incentivan la resolución de los problemas utilizando los conocimientos provenientes de las ciencias sociales, naturales y de la salud. El objetivo de este artículo es describir posibles retos para la Salud Ambiental, analizando similitudes y discrepancias en diferentes discursos sobre el surgimiento y resurgimiento de la antropozoonosis, a través de una revisión de la literatura científica., Medical Ecology studies how human beings adapt to their environment, analyzing the effects on their health and their interactions with ecosystems. One Health is a global strategy that relies on multidisciplinary collaboration and communication among groups from different countries with the aim of promoting health, not only of humans, but also of animals and the environment. On the other hand, EcoHealth is a movement that strives for sustainable health—both of living beings and ecosystems. Understanding this complex relationship is not easy. To this end, EcoHealth promotes studies using methodologies that encourage problem-solving using knowledge drawn from the social, natural and health sciences. The aim of this article is to describe the potential challenges Environmental Health faces and study the similarities and discrepancies in the different discourses on the emergence and re-emergence of anthropozoonoses by reviewing scientific literature.
Published: 2021

5. Bird diversity and noteworthy records from the western side of the Porculla Pass and the Huancabamba-Chamaya river sub-basin, northwest of Peru [with Erratum]

Author: Saldaña, Irwing S., División de Biogeografía y Conservación, Instituto de Ciencias Antonio Brack, Lima 15034, PERU Maria Koepcke Lab of Ornithology, Centro de Investigación en Biología Tropical y Conservación – CINBIOTYC, Calle Nestor Martos E 16, Piura 20004, PERU, Ugaz, Armando, Maria Koepcke Lab of Ornithology, Centro de Investigación en Biología Tropical y Conservación – CINBIOTYC, Calle Nestor Martos E 16, Piura 20004, PERU Escuela de Ciencias Biológicas, Universidad Nacional de Piura, Urb. Miraflflores S/N, Castilla, Piura 20002, PERÚ, Baldeón, Adalhif, Maria Koepcke Lab of Ornithology, Centro de Investigación en Biología Tropical y Conservación – CINBIOTYC, Calle Nestor Martos E 16, Piura 20004, PERU, Benites, Diego A., Barrionuevo, Robert, Escuela de Ciencias Biológicas, Universidad Nacional de Piura, Urb. Miraflflores S/N, Castilla, Piura 20002, PERÚ, Vallejos, Luis Martín, and Maria Koepcke Lab of Ornithology, Centro de Investigación en Biología Tropical y Conservación – CINBIOTYC, Calle Nestor Martos E 16, Piura 20004, PERU Laboratorio de Ecologia de Aves. IBRAG, Universidade do Estado do Rio de Janeiro, Pavilhão Haroldo Lisboa da Cunha, Sala 224, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, RJ, 20550-013, BRASIL Programa de Pós-Graduação em Ecología. Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Brigadeiro Trompowski, s/n, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, 21941- 599, BRASIL
Subjects: Endemismo, trans-Andino, noroeste de Perú, subespecies, Bosque Tropical Estacionalmente Seco Ecuatorial, hotspot
Abstract: Despite the great importance of the level of biodiversity and endemism that the EquatorialSeasonal Tropical dry Forest hosts, many of its areas remain unexplored. Here we present the resultsof the field evaluations carried out between 2014 and 2018 along the western side of the Porcullapass and the Huancabamba-Chamaya river sub-basin, in the northwest of Peru. this research is partof the dataset of the project Bird Assessments in Ecosystems of the Northwest of Peru – CINBIOTYC.We reported 170 bird taxa, belonging to 163 species and 32 families. Likewise, we reported twomigratory bird species, one boreal and one austral, four endemic of Peru, and 29 restricted-rangespecies, from which 25 belong to the Tumbesian Region, five to the Marañón Valley and one wasshared between them. We highlighted the record of four trans-Andean bird taxa, Amazilia amazilialeucophoea, Euphonia saturata, Basileuterus trifasciatus, and Pyrocephalus rubinus piurae, as well as, theremarkable records of Patagioenas oenops, Pachyramphus spodiurus, Turdus maranonicus, Incaspizaortizi, and the record of Thamnophilus bernardi at the east slope of the Andes (east of Porculla Pass).
Published: 2020

6. Bird diversity and noteworthy records from the western side of the Porculla Pass and the Huancabamba-Chamaya river sub-basin, northwest of Peru

Author: Saldaña, Irwing S., Maria Koepcke Lab of Ornithology, Centro de Investigación en Biología Tropical y Conservación – CINBIOTYC, Piura, Perú, Ugaz, Armando, Escuela de Ciencias Biológicas, Universidad Nacional de Piura, Piura, Perú, Baldeón, Adalhif, Benites, Diego A., Barrionuevo, Robert, Vallejos, Luis Martín, Laboratorio de Ecologia de Aves. IBRAG, Universidade do Estado do Rio de Janeiro, RJ, Brasil, and Programa de Pós-Graduação em Ecología. Instituto de Biologia, Universidade Federal do Rio de Janeiro, RJ, Brasil
Subjects: Endemism, trans-Andean, northwest of Peru, subspecies, Equatorial Seasonally Dry Tropical Forest, hotspot
Abstract: Despite the great importance of the level of biodiversity and endemism that theEquatorial Seasonal Tropical dry Forest hosts, many of its areas remain unexplored. Here wepresent the results of the field evaluations carried out between 2014 and 2018 along the westernside of the Porculla pass and the Huancabamba-Chamaya river sub-basin, in the northwest of Peru.this research is part of the dataset of the project Bird Assessments in Ecosystems of the Northwestof Peru – CINBIOTYC. We reported 174 bird taxa, belonging to 163 species and 32 families. Likewise,we reported two migratory bird species, one boreal and one austral, six endemics of Peru, and 31restricted-range species, from which 25 belong to the Tumbesian Region, seven to the MarañónValley and one was shared between them. We highlighted the record of four trans-Andean birdtaxa, Amazilia amazilia leucophoea, Euphonia saturata, Basileuterus trifasciatus, and Pyrocephalusrubinus piurae, as well as, the remarkable records of Patagioenas oenops, Thamnophilus shumbae,Pachyramphus spodiurus, Turdus maranonicus, and Incaspiza ortizi.
Published: 2020

7. Distribución de los agaves de México.-- p. 17-45

Author: Ramírez Laguna, Antonio, Universidad Nacional Autonoma de Mexico Instituto de Biologia, Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO), Ramírez Laguna, Antonio, and Universidad Nacional Autonoma de Mexico Instituto de Biologia
Subjects: Análisis, botánica, Maguey, Mexico
Published: 1936

8. Previous Conceptions of Students on Virology and Biotechnology: The Need for the Understanding

Author: Macena, Lorena da Graça P.; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Vieira, Nathália Regina P.; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Corrêa, Roberta Pires; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Paixão, Izabel; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Castro, Helena Carla; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Macena, Lorena da Graça P.; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Vieira, Nathália Regina P.; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Corrêa, Roberta Pires; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, Paixão, Izabel; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense, and Castro, Helena Carla; LABiEMol/PPBI, Instituto de Biologia, Universidade Federal Fluminense
Abstract: Biotechnology is known as the set of processes and techniques that involves the manipulation of living beings, resulting in the production of a series of products useful to humanity. Virology is a science that studies viruses, sub-viral particles and prions and has enjoyed the benefits of biotechnology. However, although there is an increase and improvement in the productivity of goods and services including this area, the harmful potential of the virus is still highlighted, which favors the construction of negative conceptions that may make it difficult to learn subjects related to these beings or about Content in science, such as biotechnology. The theme Biotechnology and virology in high school is addressed, throughout the different series / years, in disciplinary contents that have a direct influence with the students' daily life and that, if well understood, can contribute to the improvement of their quality of life. Considering that students have knowledge prior to formal education and that such conceptions may become obstacles to the acquisition of new knowledge, this work sought to elucidate, through the application of a semi-structured questionnaire, the knowledge that high school students of a school State of São Gonçalo (RJ) have on topics related to biotechnology and virology. The results showed that students use a lot of information acquired in formal education, in the media and in social relations on the subject evaluated. It was evidenced a predominance of previous conceptions and little knowledge about the viruses and the biotechnological context present in our daily life through the use of products and services
Published: 2017

9. Como abordar questões sociocientíficas na sala de aula: a estratégia didática de cinco fases para o ensino de ciências

Author: Conrado, Dália Melissa, Faculdade de Educação, Universidade Federal da Grande Dourados, INCT-INTREE, Universidade Federal da Bahia, Nunes-Neto, Nei, Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, El-Hani, Charbel, and Instituto de Biologia, Universidade Federal da Bahia
Subjects: metodologias participativas, educação CTSA, método de ensino, letramento científico crítico, educação científica
Published: 2019

10. Dengue, Zika and Chicungunha: The use of a mathematic concept to develop an educational game for helping on fighting important viral diseases

Author: Dutra, T.; Pós-graduação em Biociências e Saúde, IOC, FIOCRUZ, RJ, Costa, D.P.; PPBI e CMPDI, Instituto de Biologia, UFF, RJ, Barboza, C.F.S.; Pós-graduação em Biociências e Saúde, IOC, FIOCRUZ, RJ, Alves, L.; PPBI e CMPDI, Instituto de Biologia, UFF, RJ, Castro, H.C.; Pós-graduação em Biociências e Saúde, IOC, FIOCRUZ, RJ PPBI e CMPDI, Instituto de Biologia, UFF, RJ, Dutra, T.; Pós-graduação em Biociências e Saúde, IOC, FIOCRUZ, RJ, Costa, D.P.; PPBI e CMPDI, Instituto de Biologia, UFF, RJ, Barboza, C.F.S.; Pós-graduação em Biociências e Saúde, IOC, FIOCRUZ, RJ, Alves, L.; PPBI e CMPDI, Instituto de Biologia, UFF, RJ, and Castro, H.C.; Pós-graduação em Biociências e Saúde, IOC, FIOCRUZ, RJ PPBI e CMPDI, Instituto de Biologia, UFF, RJ
Abstract: Dengue is the leading cause of viral death worldwide. The vector Aedes aegypti mosquito is also responsible for Zika and Chincungunha transmission, another very compromising viral diseases. As the understanding of the vector life cycle and its habitat is important for preventing and fighting against these diseases, we propose to use a mathematic concept, graphos, and a problem-based situation (the removal of potential breeding sites for mosquitoes in the player city) to design a computational game that may help on spreading information and to stimulate a players proactive virtual and real behavior. Thus this paper describes the design and construction of an educational computer game called "Graphos against mosquitos," based on graphos, a mathematics theoretical concept. We designed the Graphos game using as the main elements: a child, twelve neighborhood blocks, mosquitoes, streets and containers where mosquitoes lay eggs (tires, cans, bottles, plants with water deposits). In the game, the player (Avatar) is the main element that should "walk in" the streets (edges) of the city, removing the potential breeding grounds for mosquitoes in each block (vertices). As this game is based on the mathematics theoretical concept graphos using two dimensions, the city has two distinct vertices that are the beginning and end of it, which discriminate the beginning and end of the game. The player should remove all containers of each street, avoiding mosquitoes growth and passing only one time for each of them. As containers are removed, the player gains points and is prevented to return to the previous street. In this game the removal of all containers that pose risk of becoming mosquito breeding sites, without coming back to the previous street but only forward, is translatable into " graphos language". When finishing the game properly, it opens a final screen where the player is awarded with a medal. On this screen, the player can write his/her name, being invited to act as a "
Published: 2016

11. Mathematics and blindness: let's try to solve this problem?

Author: T.M.M., Pinho; CMPDI, Instituto de Biologia, UFF, RJ, H.C., Castro; CMPDI, Instituto de Biologia, UFF, RJ. PPBI, Instituto de Biologia, UFF, RJ., L., Alves; PPBI, Instituto de Biologia, UFF, RJ, N.R.W., Lima; CMPDI, Instituto de Biologia, UFF, RJ, T.M.M., Pinho; CMPDI, Instituto de Biologia, UFF, RJ, H.C., Castro; CMPDI, Instituto de Biologia, UFF, RJ. PPBI, Instituto de Biologia, UFF, RJ., L., Alves; PPBI, Instituto de Biologia, UFF, RJ, and N.R.W., Lima; CMPDI, Instituto de Biologia, UFF, RJ
Abstract: According to World Health Organization, the number of people with blindness due to infectious diseases has reduced in the last 20 years whereas 80% of all visual impairment can be prevented or cured. Despite of that, 285 million people are estimated to be visually impaired worldwide as about 90% are from low-income society. Considering this scenario, it is still necessary to think about this public and know how to teach them disciplines such as Mathematics that are important to their lives as citizens. The Mathematics concepts are always in need for living in our society as we have to pay bills and considering that Mathematics knowledge is demanded all time from health to payment. In this article, we briefly discuss the blindness and the Mathematics universe, pointing this educational area as one that should be more educational developed. The article also intends to stimulate and contribute for the production of new strategies and didactical materials for teaching Mathematics to this public with special needs.
Published: 2016

12. The effect of galsulfase enzyme replacement therapy on the growth of patients with mucopolysaccharidosis VI (Maroteaux-Lamy syndrome)

Author: Harmatz P1, Hendriksz CJ2, Lampe C3, McGill JJ4, Parini R5, Leão Teles E6, Valayannopoulos V7, Cole TJ8, Matousek R9, Graham S9, Guffon N10, Quartel A9, he MPS VI Study Group co investigators were Yasmina Amraoui, Children's Hospital, Md, University of Mainz, Germany, Laila, Arash, Children's Hospital, University of Mainz, Germany, Javier Arroyo, Md, Hospital San Pedro de Alcantara, Hospital de día de Pediatría, Caceres, Spain, Ana, Cecliaíazevedo, Serviço de Genética Médica/HCPA, Md, Department of Genetics/UFRGS, Porto Alegre, Brazil, Barone, RITA MARIA ELISA, Department of Pediatrics, Md, University of Catania, Catania, Italy, Michael Beck, Md, D. N. Bennett Jones, Md, Consultant General Renal Physician, Whitehaven, Philippe Bernard, Md, Centre Hospitalier d'Arras, Arras, France, Thierry Billette de Villemeur, Hôpital Trousseau, Paris, France, Raquel, Boy, Hospital Universitário Pedro Ernesto, Md, Rio de Janeiro, Brazil, Susan, Conrad, Research Center Oakland, Oakland, Ca, Usa, Eduardo Coopman, Md, Hospital del Cobre D. e. Salvador, Calama, Chile, Agata Fiumara, Md, Department of Pediatrics, University of Catania, Catania, Italy, William, Frischman, The Townsville Hospital, Md, Townsville, Australia, Roberto, Giugliani, Phd, Md, Serviço de Genética Médica/HCPA, Department of Genetics/UFRGS, Porto Alegre, Brazil, Elio Gizzi, Md, Children's Hospital Research Center Oakland, Oakland, Usa, Ca, Paul, Harmatz, John J. Hopwood, Department of Genetic Medicine, Women'S, Children's Hospital Adelaide, North Adelaide, Australia, Simon Jones, Md, Royal Manchester Children's Hospital, Manchester, Paige Kaplan, Children's Hospital of Philadelphia, Philadelphia, Pa, Laura Keppen, Md, Department of Pediatrics, University of South Dakota School of Medicine, Sioux Falls, Sd, David Ketteridge, Department of Genetic Medicine, Prof Rudolf Korinthenberg, Universitätsklinikum Freiburg, Zentrum für Kinderheilkunde und Jugendmedizin, Klinik II Neuropädiatrie und Muskelerkrankungen, Freiburg, Germany, Michel, Kretz, Hôpital Civil de Colmar, Md, Le Parc Centre de la Mère et de l'Enfant, Colmar, Elisa Leão Teles, Md, Unidade de Doenças Metabólicas, Departamento Pediatria, Hospital de Sao João, Porto, Portugal, Claudia Lee, Mph, Shuan Pei Lin, MacKay Memorial Hospital, Md, Department of Genetics, Taipei, Taiwan, Lionel Lubitz, Md, Royal Children's Hospital, Melbourne, Ana Maria Martins, Md, Unifesp, Instituto de Oncologia Pediátrica, Graacc/unifesp, Departamento de Pediatria, São Paulo, Brazil, Clara Sá Miranda, M., Unidade de Biologia do Lisossoma e. Peroxisoma, Md, Instituto de Biologia Molecular e. Celular, Porto, Stephanie Oates, RN Department of Genetic Medicine, Anne O'Meara, Md, Our Lady's Hospital for Sick Children, Dublin, Ireland, Ans van der Ploeg, Md, Erasmus MC University Medical Center, Rotterdam, The, Netherlands, Isabel Cristina Neves de Souza, Md, Universidade Federal do Pará, Centro de Ciências Biológicas, Hospital Universitário João de Barros Barreto, Belém, Ray Pais, Md, Pediatric Hematology/Oncology, East Tennessee Children's Hospital, Knoxville, Tn, Gregory Pastores, Md, Phd, NYU Medical Center, Rusk Institute, New York, Usa, Ny, Lorenzo, Pavone, Barbara Plecko, U. n. i. v. Klinik fur Kinder und Jugendheilkunde, Graz, Austria, Silvio, Pozzi, Ospedale Vito Fazzi, Md, UO Pediatria, Lecce, Uwe Preiss, Md, Universitaetsklinik und Poliklinik fuer Kinder, Halle, Emerson Santana Santos, Md, Fundação Universidade de Ciências da Saúde de Alagoas Governador, Departamento de Pediatria, Maceió, Brazil, Maurizio, Scarpa, Department of Pediatrics, University of Padova, Padova, Italy, Schwartz, Ida Vanessa D., David, Sillence, Westmead, Australia, Luiz Carlos Santana da Silva, Phd, Universidade Federal do Pará, Centro de Ciências Biológicas, Hospital Universitário João de Barros Barreto, Belém, Brazil, Julie, Simon, Children's Hospital, Rn, Prof Giovanni Sorge, Department of Pediatrics, Robert Steiner, Departments of Pediatrics, Molecular, Medical, Genetics, Oregon Health Science University, Portland, Usa, Or, Valadares, Eugênia R., Hospital das Clínicas, Faculdade de Medicina da Universidade Federal de Minas Gerais UFMG, Avenida Professor Alfredo Balena, Belo Horizonte Minas Gerais, Bonito Victor, Md, Lewis Waber, Md, Phd, Pediatric Genetics, Metabolism, University of Texas Southwest Medical Center, Dallas, Usa, Tx, John, Waterson, Whitley, Chester B., University of Minnesota Medical School, Minneapolis, Usa, Mn, Edmond Wraith, J., Royal Manchester Children's Hospital, Md, and Manchester, U. k.
Subjects: 0301 basic medicine, Arylsulfatase B, Male, Lysosomal storage disorder, N-Acetylgalactosamine-4-Sulfatase, Endocrinology, Diabetes and Metabolism, Mucopolysaccharidosis, Growth, Biochemistry, Gastroenterology, chemistry.chemical_compound, Endocrinology, Child, Mucopolysaccharidosis VI, Age Factors, Enzyme replacement therapy, Recombinant Proteins, Diabetes and Metabolism, Galsulfase, Height, Maroteaux-Lamy syndrome, Molecular Biology, Genetics, Child, Preschool, Female, medicine.symptom, medicine.medical_specialty, Adolescent, Urinary system, Short stature, 03 medical and health sciences, Internal medicine, medicine, Humans, Enzyme Replacement Therapy, Creatinine, business.industry, Infant, Newborn, Infant, medicine.disease, Body Height, Maroteaux–Lamy syndrome, 030104 developmental biology, chemistry, Immunology, business, Follow-Up Studies
Abstract: Mucopolysaccharidosis (MPS) VI is an autosomal recessive lysosomal storage disorder arising from deficient activity of N-acetylgalactosamine-4-sulfatase (arylsulfatase B) and subsequent intracellular accumulation of the glycosaminoglycans (GAGs) dermatan sulfate and chondroitin-4-sulfate. Manifestations are multi-systemic and include skeletal abnormalities such as dysostosis multiplex and short stature. Reference height-for-age growth charts for treatment-naive MPS VI patients have been published for both the slowly and rapidly progressing populations. Categorization of disease progression for these charts was based on urinary GAG (uGAG) level; high (>200μg/mg creatinine) levels identified subjects as rapidly progressing. Height data for 141 patients who began galsulfase treatment by the age of 18years were collected and stratified by baseline uGAG level and age at ERT initiation in 3-year increments. The reference MPS VI growth charts were used to calculate change in Z-score from pre-treatment baseline to last follow-up. Among patients with high baseline uGAG levels, galsulfase ERT was associated with an increase in Z-score for those beginning treatment at 0-3, >3-6, >6-9, >9-12, and >12-15years of age (p
Published: 2017

13. On the distribution and ecology of Leposternon octostegum: Putting a subterranean reptile species on the map

Author: José Duarte de Barros Filho; Universidade Estadual do Rio de Janeiro, Instituto de Biologia Roberto Alcântara Gomes, Departamento de Zoologia, Laboratório de Zoologia de Vertebrados – Tetrapoda. Rua São Francisco Xavier 524, Maracanã. CEP 20550-013. Rio de Janeiro, RJ, Marco Antonio de Freitas; Universidade Estadual de Santa Cruz, Departamento de Biologia, Programa de pós-graduação em Zoologia. CEP 45600-000. Ilhéus, BA, Brazil., Thais Figueiredo Santos Silva; Biogeographia Publicações e Consultoria Ambiental, Rua E quadra D lote 11, Jardim Aeroporto, CEP 42700-000, Lauro de Freitas, BA, Brazil., Maria Celeste Costa Valverde; Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas. Avenida Transnordestina, S/N, Novo Horizonte, Km3 Br116 Norte, CEP: 44036-900, Feira de Santana, BA, Brazil., Mariana Fiuza de Castro Loguercio; Universidade Estadual do Rio de Janeiro, Instituto de Biologia Roberto Alcântara Gomes, Departamento de Zoologia, Laboratório de Zoologia de Vertebrados – Tetrapoda. Rua São Francisco Xavier 524, Maracanã. CEP 20550-013. Rio de Janeiro, RJ, Brazil., Diogo Veríssimo; Durrell Institute of Conservation and Ecology, University of Kent, CT2 7NR, UK Canterbury, Kent, United Kingdom., José Duarte de Barros Filho; Universidade Estadual do Rio de Janeiro, Instituto de Biologia Roberto Alcântara Gomes, Departamento de Zoologia, Laboratório de Zoologia de Vertebrados – Tetrapoda. Rua São Francisco Xavier 524, Maracanã. CEP 20550-013. Rio de Janeiro, RJ, Marco Antonio de Freitas; Universidade Estadual de Santa Cruz, Departamento de Biologia, Programa de pós-graduação em Zoologia. CEP 45600-000. Ilhéus, BA, Brazil., Thais Figueiredo Santos Silva; Biogeographia Publicações e Consultoria Ambiental, Rua E quadra D lote 11, Jardim Aeroporto, CEP 42700-000, Lauro de Freitas, BA, Brazil., Maria Celeste Costa Valverde; Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas. Avenida Transnordestina, S/N, Novo Horizonte, Km3 Br116 Norte, CEP: 44036-900, Feira de Santana, BA, Brazil., Mariana Fiuza de Castro Loguercio; Universidade Estadual do Rio de Janeiro, Instituto de Biologia Roberto Alcântara Gomes, Departamento de Zoologia, Laboratório de Zoologia de Vertebrados – Tetrapoda. Rua São Francisco Xavier 524, Maracanã. CEP 20550-013. Rio de Janeiro, RJ, Brazil., and Diogo Veríssimo; Durrell Institute of Conservation and Ecology, University of Kent, CT2 7NR, UK Canterbury, Kent, United Kingdom.
Abstract: Basic information on species distribution and ecology is essential for effective species conservation and management. However, several groups even within the vertebrates have received little attention. One of such groups is the subterranean herpetofauna which, although making up about 20% of all known reptile and amphibian species, includes many species for which virtually nothing is known. This situation is showcased by Leposternon octostegum, a worm lizard species from Brazil. In this study we present first verifiable fine scale distribution records for the species, which confirm the presence of the species in the Salvador Metropolitan region and enable the drawing of the first species distribution map. We furthermore present the first information on the species ecology, establishing its presence in a variety of habitats and soil types. Further research will need to be done to clarify aspects of the conservation biology of subterranean herpetofauna, especially as these species are likely to perform important ecosystem services. This will however be especially challenging in megadiverse countries like Brazil, which still harbor a myriad of undescribed and understudied species.
Published: 2013

14. Endocytosis and intracellular trafficking of nanomaterials

Author: Ferreira, L. A. B., Nano-Cell Interactions Lab., Departamento de Bioquimica, Instituto de Biologia CP 6109, Universidade Estadual de Campinas - UNICAMP, 13083-970, Campinas, SP, Brasil, Radaic, A., Pugliese, G. O., Valentini, M. B., Oliveira, M. R., and de Jesus, M. B.
Subjects: Endocitose, nanopartícula, tráfego intracelular, endocytosis nanoparticle, intracellular trafficking
Published: 2015

15. Investigação Química da Marthasterias Glacialis da Costa Portuguesa, como Fonte de Compostos com Actividade Farmacêutica

Author: Pereira, D.M., REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Laboratório de Bioquímica, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Ferreres, F., Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain, Valentão, P., REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Portugal, Teixeira, Natércia, Laboratório de Bioquímica, Departamento de Ciências Biológicas, Faculdade de Farmácia, IBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal, and P.B., Andrade
Subjects: bioactive molecules, Marthasterias glacialis, portuguese coast, actividade biofarmacêutica, Marthasterias glacialis, costa portuguesa
Published: 2014

16. O Sistema Endocanabinóide – uma perspetiva terapêutica

Author: Fonseca, B.M., Departamento de Ciências Biológicas, Laboratório de Bioquímica da Faculdade de Farmácia da Universidade do Porto. Portugal - Instituto de Biologia Molecular e Celular da Universidade do Porto. Portugal, Costa, M.A., Almada, M., Soares, A., Serviços Farmacêuticos, Centro Hospitalar Tâmega e Sousa – Unidade S. Gonçalo. Portugal, Correia-da-Silva, G., and Teixeira, Natércia A.
Subjects: Endocanabinóides, Recetores canabinóides, Farmacoterapia, Endocannabinoids, Cannabinoid receptors, Pharmacotherapy
Published: 2013

17. Aspetos hematológicos do envelhecimento

Author: Andrade, Rita, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal., Costa, Elísio, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal. Instituto de Biologia Molecular e Celular da Universidade do Porto, Porto, Portugal., and Santos-Silva, Alice
Subjects: anaemia, aging, inflammation, hematopoiesis, renal disease, anemia, envelhecimento, inflamação, hematopoiese, doença renal
Published: 2012

18. Cryopreservation of swine ovarian tissue: effect of different cryoprotectants on the structural preservation of preantral follicle oocytes.

Author: Universidade de Brasília - Instituto de Biologia, Borges, E N, Silva, R C, Futino, D O, Rocha-Junior, C M C, Andrade Amorim, Christiani, Báo, S N, Lucci, C M, Universidade de Brasília - Instituto de Biologia, Borges, E N, Silva, R C, Futino, D O, Rocha-Junior, C M C, Andrade Amorim, Christiani, Báo, S N, and Lucci, C M
Abstract: The present study aimed to test different cryoprotectants on cryopreservation of pig ovarian tissue. Pig ovaries (n=3) were collected at a local slaughterhouse. From each ovary, ten cortex samples were taken. One was immediately fixed (control) and another placed in short-term tissue incubation (STTI control). The other 8 samples were cryopreserved, in pairs, using 4 different cryoprotectants: dimethyl sulphoxide (Me2SO - 1.5M), ethylene glycol (EG - 1.5M), propanediol (PROH - 1.5M) and glycerol (GLY - 10%), all with 0.4% sucrose. Samples were slow cooled and stored in liquid nitrogen for 7 days. After thawing and cryoprotectant removal, one sample from each treatment was immediately fixed and the other was placed in short-term tissue incubation (STTI) for 2h and then fixed. Samples were processed for histology and transmission electron microscopy. The percentages of morphologically normal follicles (MNF) in cryopreserved tissue using Me2SO (67.0+/-4.9), EG (81.8+/-1.4) and PROH (55.9+/-9.9) were significantly lower (P<0.05) than observed in fresh control tissue (97.7+/-1.2). When ovarian tissue was cryopreserved with GLY, no morphologically normal follicles could be found (0%). After STTI, PROH showed a significantly lower percentage of MNF when compared with all other treatments and the control. After ultrastructural analysis, follicles cryopreserved with Me2SO and EG showed some small alterations, but no signs of advanced degeneration. Overall, these were similar to follicles from the control group. In conclusion, it is possible to cryopreserve preantral follicles from pig ovarian tissue using Me2SO or EG.
Published: 2009

19. Evolução da soroprevalência da Leucose Enzoótica Bovina em um rebanho bovino leiteiro universitário

Author: Meirelles, Ciro; Pontifícia Universidade Católica do Paraná (PUCPR), Dittrich, Thaís; Instituto de Biologia Molecular do Paraná (IBMP), Cipriano, Fábio; Pontifícia Universidade Católica do Paraná (PUCPR)., Ollhoff, Rüdiger Daniel; Universidade Católica do Paraná – PUCPR, Meirelles, Ciro; Pontifícia Universidade Católica do Paraná (PUCPR), Dittrich, Thaís; Instituto de Biologia Molecular do Paraná (IBMP), Cipriano, Fábio; Pontifícia Universidade Católica do Paraná (PUCPR)., and Ollhoff, Rüdiger Daniel; Universidade Católica do Paraná – PUCPR
Abstract: Determinou-se a soroprevalência do vírus da Leucose Enzoótica Bovina (LEB) em vacas adultas de um rebanho leiteiro da Pontifícia Universidade Católica do Paraná nos anos de 2000, 2003 e 2006 através da imunodifusão em ágar-gel. Avaliou-se a evolução da prevalência da infecção neste rebanho, verificando-se respectivamente uma prevalência nos animais em lactação de 63,33%, 81,08% e 55,56%. Em 2006 determinou-se a soroprevalência para a LEB de todas as faixas etárias do rebanho, verificando-se após a queda dos anticorpos colostrais uma prevalência crescente, culminando com mais de 80% de animais soropositivos após os 60 meses de idade. Discutiram-se as possíveis causas destas soroprevalências ao vírus da LEB considerando-se a particularidade deste rebanho visar o ensino e a extensão., The prevalence of Enzootic Bovine Leukosis virus infection was determined in adult cows of the catholic university of Paraná (Pontifícia Universidade Católica do Paraná) dairy herd in 2000, 2003 and 2006 using the agar-gel immunodiffusion. The prevalence of the infection in adult lactating cows was respectively, over the years, 63.33%, 81.08% and 55.56%. Serum samples of all animals were taken in 2006, observing a continuously rise in antibody prevalence over the time up to more than 80% in animals older than 60 months, after vanishing of colostral maternal antibodies. The possible causes of this high prevalence were discussed under the particular circumstances of a teaching and research farm of an university.
Published: 2009

20. Effects of storing pig ovaries at 4 or 20 degrees C for different periods of time on the morphology and viability of pre-antral follicles.

Author: Universidade de Brasília - Instituto de Biologia, Lucci, C M, Schreier, L L, Machado, G M, Andrade Amorim, Christiani, Báo, S N, Dobrinsky, J R, Universidade de Brasília - Instituto de Biologia, Lucci, C M, Schreier, L L, Machado, G M, Andrade Amorim, Christiani, Báo, S N, and Dobrinsky, J R
Abstract: The purpose of this study was to evaluate the effect of cooling ovarian tissue on pig pre-antral follicles. Ovaries were maintained in saline solution (0.9%) at 4 or 20 degrees C for 6, 12 or 18 h. After storage, pre-antral follicles were morphologically evaluated. While primordial follicles were not affected by the storage, the percentage of morphologically normal growing follicles was significantly reduced in ovarian tissue stored at 20 degrees C for 12 or 18 h. To test the viability of stored follicles, growing follicles isolated from ovaries stored at 4 degrees C for 18 h and at 20 degrees C for 6 h were cultured for 3 days. Follicles stored in either condition presented the same growth pattern in vitro as fresh follicles. We conclude that storage of pig ovaries at 4 degrees C for up to 18 h or at 20 degrees C for up to 6 h does not affect the morphology of growing follicles or their ability to grow in vitro.
Published: 2007

21. MYOGLOBIN CONTENT AND ENZYMATIC ACTIVITY OF PROCESS OF ADAPTATION TO HIGH ALTITUDE

Author: INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), REYNAFARJE, BALTAZAR, INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), and REYNAFARJE, BALTAZAR
Abstract: Quantitative determinations of myoglobin were made in the sartorius muscle of healthy human subjects, native of sea-level and high-altitude areas. The specific activity of the reduced form of diphosphopyridine nucleotide oxidase (DPNH-oxidase), DPNH- and TPNH-cytochrome C reductases, transhydrogenase, and isocitric and lactic dehydrogenases were also examined. A significantly higher myoglobin concentration was found in the muscle of the high-altitude natives as compared with sea-level residents. The enzyme systems DPNH-oxidase, TPNH-cytochrome C reductase, and transhydrogenase similarly showed a significantly higher activity in altitude residents. It was concluded that the respiratory capacity of the muscle was apparently higher in natives living at high altitude than in those living at sea level. The enhanced enzymatic activity was probably related to the higher pigment content of the skeletal muscle. Results on myoglobin determinations in several other muscles from certain sea-level patients are discussed.
Published: 1962

22. PYRIDINE NUCLEOTIDE OXIDASES AND TRANSHYDROGENASE IN ACCLIMATIZATION TO HIGH ALTITUDE

Author: INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), REYNAFARJE, BALTAZAR, INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), and REYNAFARJE, BALTAZAR
Abstract: Activity of pyridine nucleotide oxidases and transhydrogenase has been examined in heart, liver, and rectus femoris muscle of guinea pigs native of sea level and high altitude. There was an enhanced, reduced form of diphosphopyridine nucleotide oxidase (DPNH-oxidase) and transhydrogenase activity in heart and muscle from animals adapted to high altitude. The higher activity in muscle at altitude was due solely to increase in ratio of red to white portions. Both groups showed the pigmented portion twice as active as the white one. In liver, neither the DPNH-oxidase system nor the transhydrogenase was significantly changed in their activity on a fresh-weight basis. Nevertheless, the DPNH-oxidase is higher at altitude when the activity is expressed per gram of nitrogen. The reduced form of triphosphopyridine nucleotide oxidase activity was not appreciably changed in any of the tissues. It was concluded that adaptation to high altitude is associated with apparent changes in the magnitude of the electron transport pathway. Increased activity in skeletal muscle is probably related to the tissue pigment content.
Published: 1962

23. THERMAL AND METABOLIC RESPONSES TO COLD OF PERUVIAN INDIANS NATIVE TO HIGH ALTITUDE

Author: INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), Elsner,Robert W., Bolstad,Atle, INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), Elsner,Robert W., and Bolstad,Atle
Abstract: Thermal and metabolic responses of eight male Peruvian Indians native to an altitude of 4000 to 4500 meters were studied during nightlong exposures to moderate outdoor cold (2 to 5C). In addition, four of the subjects were studied during cold exposure while chewing coca leaves, alleged by them to have protective effects during cold exposure. The metabolic rate of the eight subjects was elevated during cold exposure but did not differ significantly from averaged values for 17 Caucasian control subjects studied under similar conditions in previous experiments. Differences between the thermal responses of the Indian subjects and of the same Caucasian controls were noted in the generally higher hand and foot temperatures, and the correspondingly lower rectal temperatures, of the high altitude dwellers. The Indian subjects using coca did not display any measurable differences in their responses with or without the drug but, by observation and by their own report, they did sleep longer and more comfortably when using coca. (Author)
Published: 1963

24. THE INFLUENCE OF HIGH ALTITUDES ON THE ELECTRICAL ACTIVITY OF THE HEART. ELECTROCARDIOGRAPHIC AND VECTORCARDIOGRAPHIC OBSERVATIONS IN ADOLESCENCE AND ADULTHOOD

Author: INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), PENALOZA, DANTE, GAMBOA, RAUL, INSTITUTO DE BIOLOGIA ANDINA LIMA (PERU), PENALOZA, DANTE, and GAMBOA, RAUL
Published: 1962

25. SCHISTOSOMIASIS DRUG SCREENING.

Author: MINAS GERAIS UNIV BELO HORIZONTE (BRAZIL) INSTITUTO DE BIOLOGIA, Pellegrino,Jose, MINAS GERAIS UNIV BELO HORIZONTE (BRAZIL) INSTITUTO DE BIOLOGIA, and Pellegrino,Jose
Abstract: After a preliminary toxicity test, 1,005 WR compounds were tested in mice experimentally infected with Schistosoma mansoni. The oogram method was used for assessing antischistosomal activity. Fifteen WR compounds displayed antischistosomal activity in mice: Triphenyl bismuth; Quinine, derivatives and isomers (10 compounds); Thiosinamine; Diaminodiphenyl sulfone; 4,4 diformamido diphenylsulfone; and one Quinoline methanol. Some of the active compounds were further studied in mice, hamsters and Cebus monkeys experimentally infected with S. mansoni. Besides oogram changes, the hepatic shift of worms was also used for the evaluation of antischistosomal activity. The results are discussed. (Author)
Published: 1968

26. BASIC BIOLOGY AND TROPISTIC BEHAVIOR OF SCHISTOSOMA MANSONI CERCARTA RELATING TO CERCARICIDAL AGENTS AND CERCARIAL REPELLENTS.

Author: MINAS GERAIS UNIV BELO HORIZONTE (BRAZIL) INSTITUTO DE BIOLOGIA, PELLEGRINO, J., MINAS GERAIS UNIV BELO HORIZONTE (BRAZIL) INSTITUTO DE BIOLOGIA, and PELLEGRINO, J.
Abstract: In the search for plant oils as protective agents against the infection of mice by Schistosoma mansoni cercariae, two additional oils (Fennel and Vetiver oils) were found highly effective (Pterodon oil had already been reported as active). The cheek pouch of the hamster is being used for in vivo studies of the mechanisms involved in the protection afforded by these active oils. No protection has been conferred by cedrene and caryophylene - the chief known constituents of Pterodon oil - to mice against the infection by S. mansoni cercariae. Fractionating studies of Pterodon oil are in progress. Remarkable cercaricidal activity is displayed by Pterodon oil. Cedrene slightly affects S. mansoni cercariae and caryophylene was found to be inactive. Pterodon oil does not possess any systemic activity on mature schistosome infections in mice. Three proteolytic components were obtained by column chromatography from S. mansoni cercarial extracts. The major proteolytic fraction shows a chymotryptic like specificity on synthetic substrates. Inhibitors of alpha chymotrypsin also inhibit the cercarial enzyme. The cutaneous response to the purified cercarial enzyme complex is being investigated. Laboratory and field experiments demonstrated that the guppy (Lebistes reticulatus) is an active predator of S. mansoni cercariae and that under special conditions it represents an important limiting factor to the infection of vertebrate hosts. It was shown that most of molluscicides in current use are remarkably toxic for the guppy. Some problems related to the biological control of schistosomiasis snail vectors are discussed. (Author)
Published: 1966

27. Acto-myosin force organization modulates centriole separation and PLK4 recruitment to ensure centriole fidelity

Author: Helder Maiato, Irène Wang, Amel Mettouchi, Jorge G Ferreira, Philippe Moreau, Elisa Vitiello, Martial Balland, Vanessa Nunes, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Chromosome Instability and Dynamics Laboratory, Universidade do Porto-Instituto de Biologia Molecular e Celular, Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto, Toxines bactériennes - Bacterial Toxins, Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC (UMR_7057)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), This work was funded by grants from the ANR, Arc, PHC-Pessoa Campus France/Fundação para a Ciência e Tecnologia, FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalization (POCI), Portugal 2020, and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the project PTDC/BEX-BCM/1758/2014 (POCI-01–0145-FEDER-016589). This work has been partially supported by the LabeX Tec 21 (Investissements d’Avenir: grant agreement No. ANR-11-LABX-0030)., ANR-11-LABX-0030,TEC XXI,Ingénierie de la Complexité : la mécanique et ses interfaces au service des enjeux sociétaux du 21iè(2011), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Universidade do Porto [Porto]-Instituto de Biologia Molecular e Celular, Universidade do Porto [Porto], Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0030/11-LABX-0030,TEC XXI,Ingénierie de la Complexité : la mécanique et ses interfaces au service des enjeux sociétaux du 21iè(2011), Instituto de Investigação e Inovação em Saúde, Universidade do Porto = University of Porto-Instituto de Biologia Molecular e Celular, Universidade do Porto = University of Porto, Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), and Matière et Systèmes Complexes (MSC)
Subjects: 0301 basic medicine, Intravital Microscopy, Centriole, General Physics and Astronomy, 02 engineering and technology, Myosin, Thymidine / pharmacology, Time-Lapse Imaging / methods, lcsh:Science, Actins / metabolism, Centrioles, Microscopy, Confocal, Multidisciplinary, Cell Cycle, Centriole duplication, 021001 nanoscience & nanotechnology, Cell biology, 0210 nano-technology, PLK4, Cell Cycle / physiology, Science, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], macromolecular substances, Myosins, Protein Serine-Threonine Kinases, Biology, Cell Cycle / drug effects, Time-Lapse Imaging, Article, General Biochemistry, Genetics and Molecular Biology, Centrioles / physiology, Myosins / physiology, 03 medical and health sciences, Centrioles / metabolism, Humans, Centrosome duplication, Myosins / metabolism, Actins / physiology, Protein-Serine-Threonine Kinases / physiology, General Chemistry, Aneuploidy, Actins, 030104 developmental biology, Centrosome, Intravital Microscopy / methods, lcsh:Q, Protein-Serine-Threonine Kinases / metabolism, HeLa Cells, Thymidine
Abstract: The presence of aberrant number of centrioles is a recognized cause of aneuploidy and hallmark of cancer. Hence, centriole duplication needs to be tightly regulated. It has been proposed that centriole separation limits centrosome duplication. The mechanism driving centriole separation is poorly understood and little is known on how this is linked to centriole duplication. Here, we propose that actin-generated forces regulate centriole separation. By imposing geometric constraints via micropatterns, we were able to prove that precise acto-myosin force arrangements control direction, distance and time of centriole separation. Accordingly, inhibition of acto-myosin contractility impairs centriole separation. Alongside, we observed that organization of acto-myosin force modulates specifically the length of S-G2 phases of the cell cycle, PLK4 recruitment at the centrosome and centriole fidelity. These discoveries led us to suggest that acto-myosin forces might act in fundamental mechanisms of aneuploidy prevention., Centriolar separation is thought to be crucial for centriole duplication, but the mechanism behind separation is poorly understood. Here, using micropatterning, the authors report that actomyosin forces influence the direction, distance, and time of centriole separation.
Published: 2019
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28. Mucosal T follicular helper cells in SIV-infected rhesus macaques: contributing role of IL-27

Author: Calayselvy Soundaramourty, Henintsoa Rabezanahary, Félicien Moukambi, Jérôme Estaquier, Ouafa Zghidi-Abouzid, Mireille Laforge, Julien Clain, Ghita Benmadid-Laktout, Yasmina Fortier, Vasco Rodrigues, Parasite Disease Group, Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde-Universidade do Porto [Porto], Mort cellulaire programmée et Signalisation (FR 3636 ), Fédération de Recherche en Neurosciences (FR 3636), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biochimie et biophysique moléculaire et cellulaire (IBBMC), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Universidade do Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), and CNRS FR3636, Faculty of Medecine des Saint-Pères, Paris Descartes University , Paris , France.
Subjects: 0301 basic medicine, Interleukin-27, [SDV]Life Sciences [q-bio], Immunology, Simian Acquired Immunodeficiency Syndrome, Gene Expression, Biology, medicine.disease_cause, Article, Immunophenotyping, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Animals, Immunology and Allergy, Mesenteric lymph nodes, Lymphocyte Count, RNA, Messenger, Phosphorylation, Interleukin 27, Intraepithelial Lymphocytes, STAT5, ComputingMilieux_MISCELLANEOUS, B-Lymphocytes, Germinal center, T-Lymphocytes, Helper-Inducer, Simian immunodeficiency virus, Immunohistochemistry, Macaca mulatta, 3. Good health, 030104 developmental biology, medicine.anatomical_structure, biology.protein, Intraepithelial lymphocyte, [SDV.IMM]Life Sciences [q-bio]/Immunology, Simian Immunodeficiency Virus, Lymph Nodes, Immunologic Memory, Biomarkers, 030215 immunology
Abstract: International audience; Mesenteric lymph nodes (MLNs), that drain the large and small intestine, are critical sites for the induction of oral tolerance. Although depletion of CD4 T cells in the intestinal lamina propria is a hallmark of HIV infection, CD4 T cell dynamics in MLNs is less known due to the lack of accessibility to these LNs. We demonstrate the early loss of memory CD4 T cells, including T follicular helper cells (Tfh) and a remodeling of MLN architecture in SIV-infected rhesus macaques (RMs). Along with the loss of Tfh cells, we observe the loss of memory B cells and of germinal center B cells. Tfh cells display a Th1 profile with increased levels of the transcription factors that negatively impact on Tfh differentiation and of Stat5 phosphorylation. MLNs of SIV-infected RMs display lower mRNA transcripts encoding for IL-12, IL-23, and IL-35, whereas those coding for IL-27 are not impaired in MLNs. In vitro, IL-27 negatively impacts on Tfh cells and recapitulates the profile observed in SIV-infected RMs. Therefore, early defects of memory CD4 T cells, as well of Tfh cells in MLNs, which play a central role in regulating the mucosal immune response, may have major implications for Aids.
Published: 2019
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29. Targeting the delivery of dietary plant bioactives to those who would benefit most: from science to practical applications

Author: Nada Knežević, Anna Marja Aura, Baukje de Roos, María Teresa Garcia Conesa, Maria Rosário Bronze, Dragan Milenkovic, Arno Greyling, Tom Van de Wiele, Christine Morand, Aedin Cassidy, Jim Kaput, Ana Rodriguez-Mateos, Paul A. Kroon, Rikard Landberg, Eileen R. Gibney, Zohar Kerem, Claudine Manach, Francisco A. Tomás-Barberán, The Rowett Research Institute, University of Aberdeen, VTT Technical Research Centre of Finland (VTT), Instituto de Biologia Experimental e Tecnológica, Department of Nutrition and Preventive Medicine, Norwich Medical School, University of East Anglia, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), UCD Institute of Food and Health, University College Dublin (UCD), Unilever Research and Development, Vydiant, The Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Podravka d.d, Quadram Institute Bioscience, Division of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of Technology, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Department of Nutritional Sciences, Mount Sinai Hospital (MSH), King’s College London, Center for Microbial Ecology and Technology (CMET), Gent University, Instituto de Biologia Experimental e Tecnológica (IBET), Centro de Edafologia y Biologia aplicada del Segura (CEBAS - CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Chalmers University of Technology [Gothenburg, Sweden], Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Mount Sinai Hospital [Toronto, Canada] (MSH), King‘s College London, Universiteit Gent = Ghent University [Belgium] (UGENT), University of East Anglia [Norwich] (UEA), Quadram Institute Bioscience [Norwich, U.K.] (QIB), Biotechnology and Biological Sciences Research Council (BBSRC), Universiteit Gent = Ghent University (UGENT), and de Roos, B.
Subjects: Agriculture and Food Sciences, 0301 basic medicine, and promotion of well-being, Food industry, Phytochemicals, Medicine (miscellaneous), Review, Gut flora, Cardiovascular, Oral and gastrointestinal, 0302 clinical medicine, Stakeholders, Cost action, Marketing, Cancer, 2. Zero hunger, Potential impact, education.field_of_study, Nutrition and Dietetics, biology, Dietary intake, Diet, Vegetarian, Cardiometabolic diseases, 3. Good health, Cardiovascular Diseases, Alimentation et Nutrition, HEALTH, Healthy diet Cardiometabolic diseases Inter-individual variability in responses Stakeholders Food industry, BIOMARKERS, Population, 030209 endocrinology & metabolism, Health Promotion, METABOLISM, 03 medical and health sciences, Vegetarian, Metabolic Diseases, Inter-individual variability in responses, Food and Nutrition, Humans, Obesity, 3.3 Nutrition and chemoprevention, education, Metabolic and endocrine, Nutrition, Healthy diet, 030109 nutrition & dietetics, Nutrition & Dietetics, business.industry, Prevention, Clinical study design, Prevention of disease and conditions, biology.organism_classification, Diet, Business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: Background A healthy diet and optimal lifestyle choices are amongst the most important actions for the prevention of cardiometabolic diseases. Despite this, it appears difficult to convince consumers to select more nutritious foods. Furthermore, the development and production of healthier foods do not always lead to economic profits for the agro-food sector. Most dietary recommendations for the general population represent a “one-size-fits-all approach” which does not necessarily ensure that everyone has adequate exposure to health-promoting constituents of foods. Indeed, we now know that individuals show a high variability in responses when exposed to specific nutrients, foods, or diets. Purpose This review aims to highlight our current understanding of inter-individual variability in response to dietary bioactives, based on the integration of findings of the COST Action POSITIVe. We also evaluate opportunities for translation of scientific knowledge on inter-individual variability in response to dietary bioactives, once it becomes available, into practical applications for stakeholders, such as the agro-food industry. The potential impact from such applications will form an important impetus for the food industry to develop and market new high quality and healthy foods for specific groups of consumers in the future. This may contribute to a decrease in the burden of diet-related chronic diseases., Key messages Individual differences in ADME (Absorption, Digestion, Metabolism and Excretion) is believed to underpin much of the inter-individual variation in responses.Recent developments in the area of food metabolome databases and fast improvements in innovative metabotyping technologies hold great promise for improved profiling of dietary intake, exposure to individual ingredients, foods and dietary patterns, as well as our ability to identify individual responsiveness.The food industry needs well-defined population clusters or targets in order to be able to design “personalized products”.There are indeed excellent industrial opportunities for foods that modulate gut microbiota, and thereby enable the delivery of food bioactive metabolites.It is currently not clear whether knowledge on individual nutrient needs, based on genetic or metagenomic data, would affect long-term dietary and health behaviours.Data to support the development of dietary recommendations may need to be generated by new n-of-1-based study designs in the future.
Published: 2019
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30. The harmonized INFOGEST in vitro digestion method: From knowledge to action

Author: Neil M. Rigby, Lonneke M. JanssenDuijghuijsen, Didier Dupont, Irene Comi, Beatriz Miralles, Anne Meynier, Helena Stoffers, Paula Alvito, Anne Pihlanto, Laurie Eve Rioux, Sibel Karakaya, Brent S. Murray, Ana Tavares, María Jesús Lagarda, Steven Le Feunteun, Guadalupe Garcia-Llatas, Sebnem Simsek, Alfonso Clemente, Isidra Recio, Alan R. Mackie, Gianluca Picariello, Lotti Egger, Ellen Kathrine Ulleberg, Olivia Ménard, Reto Portmann, Reyes Barberá, André Brodkorb, Gerd E. Vegarud, Cristina Delgado-Andrade, Lucélia Tavares, Uri Lesmes, Sylvie L. Turgeon, Carla Martins, Guy Vergères, Simon Balance, Thomas Cattenoz, Ricardo Assunção, Cláudia N. Santos, Agroscope, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Department of Physiology and Biochemistry of Animal Nutrition, Estacion Experimental del Zaidin, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge I.P., National Institute of Health Doutor Ricardo Jorge, I.P, Nofima AS, Nutrition and Food Science Area, University of Valencia, Teagasc Food Research Centre [Fermoy, County Cork, Ireland], Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Wageningen University and Research Centre, Food and Biobased Research, Ege University, Engineering Faculty Department of Food Engineering, Department of Biotechnology and Food Engineering, Technion, Israel Institute of Technology, Institute of Food Research, National Institute of Health Doutor Ricardo Jorge, I.P., Food and Nutrition Department, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Instituto de Investigaciòn en Ciencias de la Alimentaciòn (CIAL, CSIC-UAM), School of Food Science and Nutrition, University of Leeds, Natural Resource Institute Finland (LUKE) New Business Opportunities, Institute of Food Sciences, National Research Council of Italy (CNR), Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (NOVA), iBET, Instituto de Biologia Experimental e Tecnológica, Apartado, Institute of Food Research, Norwich, STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Chercheur indépendant, Cost Infogest, COST Action FA1005 INFOGEST, MycoMix project, Fundação para a Ciência e Tecnologia, iNOVA4Health, PTDC/DTP-FTO/0417/2012, UID/Multi/04462/2013, IF/01097/2013, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Teagasc Food Research Centre [Fermoy, Ireland], AgroParisTech-Institut National de la Recherche Agronomique (INRA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Instituto de Biologia Experimental e Tecnológica (IBET), and Ege Üniversitesi
Subjects: harmonisation de méthode, Harmonized IVD protocol, Hydrolyzed protein, partage de connaissance, connaissance partagée, Regulation of gastric function, méthode de recherche, digestion, 0404 agricultural biotechnology, Pepsin, digestion in vitro, Inter-laboratory trial, INFOGEST network, Levensmiddelenchemie, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Medicine, Knowledge to action, Cost action, Food research, Food science, Food, Health & Consumer Research, 2. Zero hunger, collaboration scientifique, biology, Food Chemistry, Mass spectrometry, business.industry, In vitro digestion, collaboration européenne, 04 agricultural and veterinary sciences, 040401 food science, scientific co-operation, Composição dos Alimentos, research method, Health & Consumer Research, Biochemistry, Food, biology.protein, Dairy proteins, Digestion, business, Peptides, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, méthode d'harmonisation, Food Science
Abstract: 4th International Conference on Food Digestion -- MAR, 2015 -- Naples, ITALY, WOS: 000384789000006, Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas beta-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The present work gives an overview of all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future. (C) 2015 The Authors. Published by Elsevier Ltd.
Published: 2016
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31. Tracking atmospheric dispersion of metals in Rio de Janeiro Metropolitan region (Brazil) with epiphytes as bioindicators

Author: Douglas P. Panetto, Rémi Losno, Aguinaldo Nepomuceno Marques Júnior, Fabrice Monna, Rodrigue Guillon, Fernando Lamego, F.O. Nepomuceno, Programa de Biologia Marinha e Ambientes Costeiros, Departamento de Biologia Marinha, Universidade Federal Fluminense [Rio de Janeiro] (UFF)-Instituto de Biologia-Universidade Federal Fluminense [Rio de Janeiro] (UFF)-Instituto de Biologia, Departamento de Geologia, Universidade Federal do Rio de Janeiro (UFRJ)-Instituto de Geociencias, Archéologie, Terre, Histoire, Sociétés [Dijon] (ARTeHiS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Ministère de la Culture et de la Communication (MCC), Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal do Rio de Janeiro [Rio de Janeiro] (UFRJ)-Instituto de Geociencias, Ministère de la Culture et de la Communication (MCC)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-IPG PARIS-Institut national des sciences de l'Univers (INSU - CNRS)
Subjects: 010504 meteorology & atmospheric sciences, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, Lichens, Atmospheric circulation, 010501 environmental sciences, Atmospheric sciences, Dispersion (geology), 01 natural sciences, Parmotrema crinitum (Ach.), Tillandsia usneoides (L.), Stable lead isotopes, Lichen, lcsh:Science, 0105 earth and related environmental sciences, Air Pollutants, Multidisciplinary, Tillandsia, biology, Environmental Biomarkers, atmospheric pollution, 15. Life on land, Atmospheric dispersion modeling, biology.organism_classification, Plume, 13. Climate action, Metals, [SHS.ENVIR]Humanities and Social Sciences/Environmental studies, Environmental science, lcsh:Q, Epiphyte, Bioindicator, Brazil, Environmental Monitoring
Abstract: International audience; The atmospheric dispersion atmospheric plume of the metropolitan region and neighborhoods of the city of Rio de Janeiro was investigated through elemental analyzes (Na, K, Al, Mn, Pb, Ni, Cr) and stable lead isotopes in two epiphytes; the lichen Parmotrema crinitum (Ach.) and the bromeliad Tillandsia usneoides (L.). All the elements had lower concentrations in epiphytes than in rocks of the local geological basement, with the exception of K, which was similar to rocks. This behavior was attributed to the nutritional essentiality and abundance of this element in the environment. The concentrations of Na, Pb and the 206Pb/207Pb and 208Pb/206Pb ratios indicated the presence of spatial gradients in the path (a) sea shore → continent, and (b) urban center → farmlands. These patterns were associated with the mesoscale atmospheric circulation regime, in which, during the arrival of cold fronts, air masses from the metropolitan region of Rio de Janeiro are transported towards the mountainous region of Teresópolis, which acts as a barrier to the dispersion of pollutants. The bromeliad Tillandsia usneoides and the lichen Parmotrema crinitum were linked to Na and Pb atmospheric levels, respectively.
Published: 2018
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32. Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases

Author: Daniel da Rosa Farias, Alice G. Martins, Nelson J. M. Saibo, Isabel A. Abreu, Diego M. Almeida, M. Margarida Oliveira, Antonio Costa de Oliveira, Inês S. Pires, Margarida T.G. Rosa, Luciano Carlos da Maia, Abreu, Isabel A., Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Instituto de Biologia Experimental e Tecnológica (IBET), Frontiers Media SA, Plant Genomics and Breeding Center, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas = Federal University of Pelotas (UFPel), Bioresources 4 Sustainability (GREEN-IT), Universidade Nova de Lisboa (NOVA), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and iBET, Instituto de Biologia Experimental e Tecnológica, Apartado
Subjects: 0106 biological sciences, 0301 basic medicine, SUMO-1 Protein, SUMO protein, Plant Science, Genes, Plant, 01 natural sciences, SUMO proteases, 03 medical and health sciences, Gene Expression Regulation, Plant, lcsh:Botany, Arabidopsis, Rice (Oryza sativa), cis-elements, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Post-transcriptional regulation, Gene, Phylogeny, Plant Proteins, 2. Zero hunger, Oryza sativa, Vegetal Biology, biology, Alternative splicing, sumoylation, food and beverages, Oryza, protéase, biology.organism_classification, Phenotype, SUMOylation, T-DNA, lcsh:QK1-989, Cell biology, arabidopsis, 030104 developmental biology, oryza sativa, RNA splicing, Biologie végétale, Research Article, Peptide Hydrolases, 010606 plant biology & botany
Abstract: Background SUMOylation is an essential eukaryotic post-translation modification that, in plants, regulates numerous cellular processes, ranging from seed development to stress response. Using rice as a model crop plant, we searched for potential regulatory points that may influence the activity of the rice SUMOylation machinery genes. Results We analyzed the presence of putative cis-acting regulatory elements (CREs) within the promoter regions of the rice SUMOylation machinery genes and found CREs related to different cellular processes, including hormone signaling. We confirmed that the transcript levels of genes involved in target-SUMOylation, containing ABA- and GA-related CREs, are responsive to treatments with these hormones. Transcriptional analysis in Nipponbare (spp. japonica) and LC-93-4 (spp. indica), showed that the transcript levels of all studied genes are maintained in the two subspecies, under normal growth. OsSUMO3 is an exceptional case since it is expressed at low levels or is not detectable at all in LC-93-4 roots and shoots, respectively. We revealed post-transcriptional regulation by alternative splicing (AS) for all genes studied, except for SUMO coding genes, OsSIZ2, OsOTS3, and OsELS2. Some AS forms have the potential to alter protein domains and catalytic centers. We also performed the molecular and phenotypic characterization of T-DNA insertion lines of some of the genes under study. Knockouts of OsFUG1 and OsELS1 showed increased SUMOylation levels and non-overlapping phenotypes. The fug1 line showed a dwarf phenotype, and significant defects in fertility, seed weight, and panicle architecture, while the els1 line showed early flowering and decreased plant height. We suggest that OsELS1 is an ortholog of AtEsd4, which was also supported by our phylogenetic analysis. Conclusions Overall, we provide a comprehensive analysis of the rice SUMOylation machinery and discuss possible effects of the regulation of these genes at the transcriptional and post-transcriptional level. We also contribute to the characterization of two rice SUMO proteases, OsELS1 and OsFUG1. Electronic supplementary material The online version of this article (10.1186/s12870-018-1547-3) contains supplementary material, which is available to authorized users.
Published: 2018
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33. Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2

Author: PINHEIRO, A. F., ROLOFF, B. C., MOREIRA, A. da S., BERNE, M. E. A., ANDREOTTI, R., LEITE, F. P. L., Amanda Fernandes Pinheiro, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel, Angelita da Silveira Moreira, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel, RENATO ANDREOTTI E SILVA, CNPGC, Fábio Pereira Leivas Leite, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas – UFPel., Maria Elisabeth Aires Berne, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel, and Bárbara Couto Roloff, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel
Subjects: Vaccines, Cytokines, Proteins, Adjuvants, Neospora Caninum
Abstract: The parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis. Made available in DSpace on 2018-12-20T23:42:13Z (GMT). No. of bitstreams: 1 Identificationofsuitableadjuvantforvaccine.pdf: 757006 bytes, checksum: 58f3997d59d3635ff7d47d9dca5a2dc7 (MD5) Previous issue date: 2018-12-18
Published: 2018

34. A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection

Author: Paula Ferreira, Camila C. Portugal, Arnaud Firon, Ana Puga, Patrick Trieu-Cuot, Joana Bravo, Adília Ribeiro, Elva Bonifácio Andrade, Madalena Costa, Augusto Faustino, Teresa Summavielle, Margarida Correia-Neves, Ana Magalhães, Repositório Científico do Instituto Politécnico do Porto, Instituto de Investigação e Inovação em Saúde, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto = University of Porto, Instituto de Investigação e Inovação em Saúde (I3S), Instituto de Biologia Molecular e Celular - institute for molecular and cell biology [Porto, Portugal] (IBMC), Instituto Politécnico do Porto = Polytechnic Institute of Porto, Instituto de Biologia Molecular e Celular (IBMC), Life and Health Sciences Research Institute [Braga] (ICVS), University of Minho [Braga], Karolinska Institutet [Stockholm], Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by funds from Foundation for Science and Technology (FCT), European Regional Development Fund (FEDER) and Compete under project POCI-01-0145-FEDER-016607 (PTDC/IMI-MIC/1049/2014) and from the project NORTE-01-0145-FEDER-000012, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). T.S. and A.M. were supported by Investigador FCT (IF/00875/2012 and IF/00753/2014), POPH and Fundo Social Europeu. E.B.A. and C.C.P. hold postdoctoral fellowships from FCT (PTDC/IMI-MIC/1049/2014 and SFRH/BPD/91962/2012). Ar.F. and P.T.C. were supported by Laboratoire d’Excellence (LABEX) Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Universidade do Porto, Instituto Politécnico do Porto = Oporto Polytechnic Institute, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Instituto de Ciências Biomédicas Abel Salazar, and Universidade do Minho
Subjects: Male, MESH: Inflammation, 0301 basic medicine, [SDV]Life Sciences [q-bio], General Physics and Astronomy, medicine.disease_cause, MESH: Animals, Newborn, Group B, Hemolysin Proteins, Mice, MESH: Pregnancy, MESH: Streptococcal Infections, Pregnancy, MESH: Behavior, Animal, MESH: Animals, lcsh:Science, Mice, Inbred BALB C, Multidisciplinary, Behavior, Animal, Transmission (medicine), MESH: Pregnancy, Animal, 3. Good health, MESH: Infectious Disease Transmission, Vertical, MESH: Meningitis, Bacterial, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Hemolysin Proteins, Vagina, Female, Meningitis, Offspring, Science, MESH: Mice, Inbred BALB C, Group B Streptococcal Infection, Virulence, Biology, Article, General Biochemistry, Genetics and Molecular Biology, Meningitis, Bacterial, Streptococcus agalactiae, 03 medical and health sciences, MESH: Meningitis, Streptococcal Infections, medicine, Animals, Maze Learning, MESH: Mice, Inflammation, Science & Technology, Perforin, MESH: Maze Learning, Body Weight, General Chemistry, medicine.disease, MESH: Streptococcus agalactiae, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, Infectious Disease Transmission, Vertical, MESH: Perforin, MESH: Body Weight, Disease Models, Animal, 030104 developmental biology, Animals, Newborn, MESH: Vagina, Immunology, Pregnancy, Animal, lcsh:Q, Cytolysin, MESH: Disease Models, Animal, MESH: Female
Abstract: Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis., The authors gratefully acknowledge the help of Encarnaca̧ ̃o Ribeiro for excellent technical assistance, Joana Tavares for assisting with IVIS Lumina LT, Susana Roque for the luminex instrument experiments, the Molecular Microbiology group at i3S for microscope use, and the Portuguese architect and artist Gil Ferreira da Silva for the artworks included in the last figure. This work was supported by funds from Foundation for Science and Technology (FCT), European Regional Development Fund (FEDER) and Compete under project POCI-01-0145-FEDER-016607 (PTDC/IMI-MIC/1049/2014) and from the project NORTE-01-0145-FEDER-000012, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). T.S. and A.M. were supported by Investigador FCT (IF/00875/2012 and IF/00753/2014), POPH and Fundo Social Europeu. E.B.A. and C.C.P. hold postdoctoral fellowships from FCT (PTDC/IMI-MIC/1049/2014 and SFRH/BPD/91962/2012). Ar.F. and P.T.C. were supported by Laboratoire d’Excellence (LABEX) Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID)., info:eu-repo/semantics/publishedVersion
Published: 2018
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35. Challenges in Antibody Development against Tn and Sialyl-Tn Antigens

Author: Ana Barbas, Carlos Novo, Mylène A. Carrascal, Liliana R. Loureiro, José S. Ramalho, Philippe Delannoy, Paula A. Videira, Centro de Estudos de Doenças Crónicas (CEDOC), Instituto de Higiene e Medicina Tropical (IHMT), DCV - Departamento de Ciências da Vida, UCIBIO - Applied Molecular Biosciences Unit, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Instituto de Biologia Experimental e Tecnológica (IBET), NOVA Medical School - Faculdade de Ciências Médicas (NMS), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Centro de Recursos Microbiológicos (CREM), Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), CNRS, Université de Lille, Instituto de Biologia Experimental e Tecnológica [IBET], Universidade Nova de Lisboa = NOVA University Lisbon [NOVA], Centro de Estudos de Doenças Crónicas [CEDOC], and Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Subjects: Phage display, medicine.drug_class, medicine.medical_treatment, Tn antigen, lcsh:QR1-502, Sialyl Tn antigen, therapeutic antibodies, Review, Monoclonal antibody, Biochemistry, lcsh:Microbiology, immune response, Antibodies, 03 medical and health sciences, 0302 clinical medicine, Immune system, Antigen, SDG 3 - Good Health and Well-being, Neoplasms, medicine, Journal Article, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Antigens, Tumor-Associated, Carbohydrate, Molecular Biology, 030304 developmental biology, antibody production, 0303 health sciences, biology, Research Support, Non-U.S. Gov't, Immunotherapy, 3. Good health, 030220 oncology & carcinogenesis, Immunology, biology.protein, Hybridoma technology, Antibody, Genetic Engineering
Abstract: PMID: 26270678 PMCID:4598775 The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to their low immunogenicity. Alternatively, anti-Tn and/or STn antibodies may be used to harness the immune system against tumor cells. Whilst the development of antibodies against these antigens had a boost two decades ago for diagnostic use, so far no such antibody entered into clinical trials. Possible limitations are the low specificity and efficiency of existing antibodies and that novel antibodies are still necessary. The vast array of methodologies available today will allow rapid antibody development and novel formats. Following the advent of hybridoma technology, the immortalization of human B cells became a methodology to obtain human monoclonal antibodies with better specificity. Advances in molecular biology including phage display technology for high throughput screening, transgenic mice and more recently molecularly engineered antibodies enhanced the field of antibody production. The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment. publishersversion published
Published: 2015

36. Perturbation vectors to evaluate air quality using lichens and bromeliads: a Brazilian case study

Author: A.N. Marques, Fabrice Monna, Gaetano Dongarra, Daniela Varrica, Nicolas Navarro, Rémi Losno, Carmela Chateau, F.O. Nepomuceno, Rodrigue Guillon, E. Tamburo, Sébastien Couette, Archéologie, Terre, Histoire, Sociétés [Dijon] ( ARTeHiS ), Ministère de la Culture et de la Communication ( MCC ) -Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Programa de Biologia Marinha e Ambientes Costeiros, Departamento de Biologia Marinha, Universidade Federal Fluminense [Rio de Janeiro] ( UFF ) -Instituto de Biologia-Universidade Federal Fluminense [Rio de Janeiro] ( UFF ) -Instituto de Biologia, Institut de Physique du Globe de Paris ( IPGP ), Institut national des sciences de l'Univers ( INSU - CNRS ) -IPG PARIS-Université Paris Diderot - Paris 7 ( UPD7 ) -Université de la Réunion ( UR ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Paléobiodiversité et Evolution ( PALEVO ), École pratique des hautes études ( EPHE ), Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Dipartimento Scienze dellaTerra e del Mare, UFR Sciences de la Vie, de la Terre et de l'Environnement (Université de Bourgogne) ( UFR SVTE ), Université de Bourgogne ( UB ), Departamento de Geologia, Universidade Federal do Rio de Janeiro [Rio de Janeiro] ( UFRJ ) -Instituto de Geociencias, Archéologie, Terre, Histoire, Sociétés [Dijon] (ARTeHiS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Ministère de la Culture et de la Communication (MCC), Universidade Federal Fluminense [Rio de Janeiro] (UFF)-Instituto de Biologia-Universidade Federal Fluminense [Rio de Janeiro] (UFF)-Instituto de Biologia, Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Paléobiodiversité et Evolution (PALEVO), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), UFR Sciences de la Vie, de la Terre et de l'Environnement (Université de Bourgogne) (UFR SVTE), Université de Bourgogne (UB), Universidade Federal do Rio de Janeiro (UFRJ)-Instituto de Geociencias, Ministère de la Culture et de la Communication (MCC)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-IPG PARIS-Institut national des sciences de l'Univers (INSU - CNRS), Universidade Federal do Rio de Janeiro [Rio de Janeiro] (UFRJ)-Instituto de Geociencias, Monna, F., Marques Jr, A., Guillon, R., Losno, R., Couette, S., Navarro, N., Dongarrà, G., Tamburo, E., Varrica, D., Chateau, C., and Nepomuceno, F.
Subjects: Pollution, Bromeliaceae, Lichens, media_common.quotation_subject, [SDE.MCG]Environmental Sciences/Global Changes, Air pollution, chemistry.chemical_element, 010501 environmental sciences, Management, Monitoring, Policy and Law, medicine.disease_cause, 01 natural sciences, 010104 statistics & probability, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry, Air Pollution, Metals, Heavy, Environmental monitoring, Biomonitoring, Parmotrema crinitum, medicine, Tillandsia usneoides, 0101 mathematics, Lichen, Air quality index, 0105 earth and related environmental sciences, General Environmental Science, media_common, CoDA, Cadmium, Air Pollutants, Tillandsia, biology, armotrema crinitum, Tillandsia usneoide, Chemistry, Metal pollution, General Medicine, biology.organism_classification, [ SDU.STU.GC ] Sciences of the Universe [physics]/Earth Sciences/Geochemistry, Settore GEO/08 - Geochimica E Vulcanologia, [ SDE.MCG ] Environmental Sciences/Global Changes, 13. Climate action, [SDU.STU.CL]Sciences of the Universe [physics]/Earth Sciences/Climatology, Environmental chemistry, [ SDU.STU.CL ] Sciences of the Universe [physics]/Earth Sciences/Climatology, Brazil, Environmental Monitoring
Abstract: 10 pages; International audience; Samples of one lichen species, Parmotrema crinitum, and one bromeliad species, Tillandsia usneoides, were collected in the state of Rio de Janeiro, Brazil, at four sites differently affected by anthropogenic pollution. The concentrations of aluminum, cadmium, copper, iron, lanthanum, lead, sulfur, titanium, zinc, and zirconium were determined by inductively coupled plasma-atomic emission spectroscopy. The environmental diagnosis was established by examining compositional changes via perturbation vectors, an underused family of methods designed to circumvent the problem of closure in any compositional dataset. The perturbation vectors between the reference site and the other three sites were similar for both species, although body concentration levels were different. At each site, perturbation vectors between lichens and bromeliads were approximately the same, whatever the local pollution level. It should thus be possible to combine these organisms, though physiologically different, for air quality surveys, after making all results comparable with appropriate correction. The use of perturbation vectors seems particularly suitable for assessing pollution level by biomonitoring, and for many frequently met situations in environmental geochemistry, where elemental ratios are more relevant than absolute concentrations.
Published: 2017
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37. High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection

Author: FLORES, J. C., MOFATTO, L. S., FREITAS-LOPES, R. do L., FERREIRA, S. S., ZAMBOLIM, E. M., CARAZZOLLE, M. F., ZAMBOLIM, L., CAIXETA, E. T., Juan Carlos Florez, BIAGRO/BioCafé/UFV, Luciana Souto Mofatto, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas, Rejane do Livramento Freitas?Lopes, BIAGRO/BioCafé/UFV, Sávio Siqueira Ferreira, BIAGRO/BioCafé/UFV, Eunize Maciel Zambolim, BIAGRO/BioCafé/UFV, Marcelo Falsarella Carazzolle, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas, Laércio Zambolim, Departamento de Fitopatologia/Universidade Federal de Viçosa, and EVELINE TEIXEIRA CAIXETA, SAPC.
Subjects: Disease resistance, Coffee rust, Biotrophic interaction, Transcriptome
Abstract: Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdTexclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee?H. vastatrix interaction over a time course in susceptible and resistant coffee plants. Made available in DSpace on 2017-12-15T23:19:15Z (GMT). No. of bitstreams: 1 Highthroughputtranscriptomeanalysis.pdf: 3011305 bytes, checksum: 8f5e7ed12af0ad3d0f2a2e76a2091c4b (MD5) Previous issue date: 2017-12-15
Published: 2017

38. Transcriptome Analysis of Leaves, Flowers and Fruits Perisperm of Coffea arabica L. Reveals the Differential Expression of Genes Involved in Raffinose Biosynthesis

Author: IVAMOTO, S. T., REIS JÚNIOR, O., DOMINGUES, D. S., SANTOS, T. B. dos, OLIVEIRA, F. F. de, POT, D., LEROY, T., VIEIRA, L. G. E., CARAZZOLLE, M. F., PEREIRA, G. A. G., PEREIRA, L. F. P., Suzana Tiemi Ivamoto, Programa de Pós-Graduação em Genética e Biologia Molecular/Centro de Ciências Bioloógicas/Universidade Estadual de Londrina - UEL, Osvaldo Reis Júnior, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas - UNICAMP, Douglas Silva Domingues, Departamento de Botânica/Instituto de Biociências de Rio Claro, Universidade Estadual Paulista - UNESP, Tiago Benedito dos Santos, Laboratório de Biotecnologia Vegetal/Instituto Agronômico do Paraná -IAPAR, Fernanda Freitas de Oliveira, Laboratório de Biotecnologia Vegetal/Instituto Agronômico do Paraná -IAPAR, David Pot, CIRAD/UMR AGAP, Thierry Leroy, CIRAD/UMR AGAP, Luiz Gonzaga Esteves Vieira, Programa de Pós Graduação em Agronomia/Universidade do Oeste Paulista - UNOESTE, Marcelo Falsarella Carazzolle, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas - UNICAMP, Gonçalo Amarante Guimarães Pereira, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas - UNICAMP, and LUIZ FILIPE PROTASIO PEREIRA, SAPC.
Subjects: Knowledge, Coffea Arábica, Raffinose, Biosynthesis
Abstract: Coffea arabica L. is an important crop in several developing countries. Despite its economic importance, minimal transcriptome data are available for fruit tissues, especially during fruit development where several compounds related to coffee quality are produced. To understand the molecular aspects related to coffee fruit and grain development, we report a largescale transcriptome analysis of leaf, flower and perisperm fruit tissue development. Illumina sequencing yielded 41,881,572 high-quality filtered reads. De novo assembly generated 65,364 unigenes with an average length of 1,264 bp. A total of 24,548 unigenes were annotated as protein coding genes, including 12,560 full-length sequences. In the annotation process, we identified nine candidate genes related to the biosynthesis of raffinose family oligossacarides (RFOs). These sugars confer osmoprotection and are accumulated during initial fruit development. Four genes from this pathway had their transcriptional pattern validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we identified ~24,000 putative target sites for microRNAs (miRNAs) and 134 putative transcriptionally active transposable elements (TE) sequences in our dataset. This C. arabica transcriptomic atlas provides an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Our results are the starting point for enhancing our knowledge about the coffee genes that are transcribed during the flowering and initial fruit development stages. Made available in DSpace on 2017-12-23T23:24:11Z (GMT). No. of bitstreams: 1 Transcriptomeanalysisofleavesflowers.pdf: 1159910 bytes, checksum: 5f3ef06fee3d068f8acaf1b6093f3d96 (MD5) Previous issue date: 2017-12-21
Published: 2017

39. Extensive recent secondary contacts between four European white oak species

Author: Jorge A. P. Paiva, Laure Villate, Carole Dossat, Catherine Bodénès, Jean-Marc Aury, Christophe Plomion, Jonathan Romiguier, Thibault Leroy, Camille Roux, Antoine Kremer, Biodiversité, Gènes et Communautés, Institut National de la Recherche Agronomique (INRA), Department of Ecology and Evolution, Université de Lausanne, Instituto de Biologia Experimental e Tecnológica, Institute of Plant Genetics, Polska Akademia Nauk (PAN), Institut de Genomique (IG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Instituto de Biologia Experimental e Tecnológica (IBET), Polska Akademia Nauk = Polish Academy of Sciences (PAN), and Université de Lausanne = University of Lausanne (UNIL)
Subjects: 0301 basic medicine, genetical analysis, polymorphisme nucléotidique simple (SNP), arbre forestier, Genetic Speciation, Physiology, [SDV]Life Sciences [q-bio], Introgression, flux de gènes, paléobotanique, Plant Science, forest tree, Article, Gene flow, paleobotany, quercus pubescens, Quercus, 03 medical and health sciences, congènere, Species Specificity, Phylogenetics, quaternaire, quercus pyrenaica, Genetic variation, calcul bayésien, pedunculate oak, Phylogeny, bayesian method, Likelihood Functions, Principal Component Analysis, biology, Ecology, Genetic Variation, Bayes Theorem, Reproductive isolation, sessile oak, 15. Life on land, biology.organism_classification, Europe, 030104 developmental biology, Evolutionary biology, quercus petraea, interfertilité, Quercus petraea, Approximate Bayesian computation, analyse génétique, quercus robur
Abstract: *Historical trajectories of tree species during the late Quaternary have been well reconstructed through genetic and palaeobotanical studies. However, many congeneric tree species are interfertile, and the timing and contribution of introgression to species divergence during their evolutionary history remains largely unknown.*We quantified past and current gene flow events between four morphologically divergent oak species (Quercus petraea, Q. robur, Q. pyrenaica, Q. pubescens), by two independent inference methods: diffusion approximation to the joint frequency spectrum (∂a∂i) and approximate Bayesian computation (ABC). For each pair of species, alternative scenarios of speciation allowing gene flow over different timescales were evaluated.*Analyses of 3524 single nucleotide polymorphisms (SNPs) randomly distributed in the genome, showed that these species evolved in complete isolation for most of their history, but recently came into secondary contact, probably facilitated by the most recent period of postglacial warming.*We demonstrated that: there was sufficient genetic differentiation before secondary contact for the accumulation of barriers to gene flow; and current European white oak genomes are a mosaic of genes that have crossed species boundaries and genes impermeable to gene flow.
Published: 2017
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40. Nuclear receptors connect progenitor transcription factors to cell cycle control

Author: Stein Aerts, Marina Naval-Sanchez, Dirk Geerts, Marta Neto, Fernando Casares, Delphine Potier, Paulo S. Pereira, Medical Biology, Ministerio de Economía y Competitividad (España), University of Leuven, Research Foundation - Flanders, Andalusian Centre for Developmental Biology (CABD), Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto, Instituto de Biologia Molecular e Celular - institute for molecular and cell biology [Porto, Portugal] (IBMC), School of Medicine, Instituto de Biologia Molecular e Celular (IBMC), Department of Medical Biology, Amsterdam University Medical Center, University of Amsterdam [Amsterdam] (UvA), School of medecine, CABD, Andalusian Centre for Developmental Biology (CABD), and Hematology laboratory
Subjects: 0301 basic medicine, [SDV]Life Sciences [q-bio], Science, Receptors, Cytoplasmic and Nuclear, Biology, Bioinformatics, Eye, Article, 03 medical and health sciences, Animals, Drosophila Proteins, Progenitor cell, Receptor, Transcription factor, Cell Proliferation, Homeodomain Proteins, Multidisciplinary, Cell growth, Gene Expression Profiling, Cell Cycle Checkpoints, Cell cycle, Cell Cycle Gene, Chromatin, Cell biology, Repressor Proteins, 030104 developmental biology, Nuclear receptor, Medicine, Drosophila, Signal transduction, Signal Transduction
Abstract: The specification and growth of organs is controlled simultaneously by networks of transcription factors. While the connection between these transcription factors with fate determinants is increasingly clear, how they establish the link with the cell cycle is far less understood. Here we investigate this link in the developing Drosophila eye, where two transcription factors, the MEIS1 homologue hth and the Zn-finger tsh, synergize to stimulate the proliferation of naïve eye progenitors. Experiments combining transcriptomics, open-chromatin profiling, motif analysis and functional assays indicate that these progenitor transcription factors exert a global regulation of the proliferation program. Rather than directly regulating cell cycle genes, they control proliferation through an intermediary layer of nuclear receptors of the ecdysone/estrogen-signaling pathway. This regulatory subnetwork between hth, tsh and nuclear receptors might be conserved from Drosophila to mammals, as we find a significant co-overexpression of their human homologues in specific cancer types., PSP is a recipient of a Portuguese “Investigator FCT” contract. Grants BFU2012-34324 and BFU2015-66040 (MINECO, Spain) to F.C. Grants from Research Foundation Flanders (FWO, grants G. 0640.13 and G. 0791.14), University of Leuven (OT/13/103) to SA. MNS was funded by a PhD fellowship from FWO.
Published: 2017
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41. Establishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant

Author: Brigitte Raynaud-Messina, Aimie Jourdan, Alain Debec, Nicolas Lecland, Clémence Dupré, Sara Moutinho-Pereira, Helder Maiato, Nicolas Malmanche, Anaïs Bouissou, Antoine Guichet, Audrey Delmas, Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre de biologie du développement (CBD), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Chromosome Instability and Dynamics Laboratory, Universidade do Porto-Instituto de Biologia Molecular e Celular, Department of Experimental Biology, Universidade do Porto-Faculdade de Medicina - Porto - Portugal, ANR 'Blanche' grant Cymempol, Blan06-3-139786, ARC grant SL220100601358, 'Ligue Contre le Cancer' grant RS11/75-34, laboratory of Andreas Merdes, ARC (4720XP023OF), Centre National de la Recherche Scientifique, Pierre Fabre Laboratorie, the laboratory of H.M. is funded by grants PTDC/SAU-GMG/099704/2008 and PTDC/SAU-ONC/112917/2009 from Fundação para a Ciência e a Tecnologia of Portugal (COMPETE-FEDER), the Human Frontier Research Program and the 7th framework program grant PRECISE from the European Research Council, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Universidade do Porto [Porto]-Instituto de Biologia Molecular e Celular, and Universidade do Porto [Porto]-Faculdade de Medicina - Porto - Portugal
Subjects: Spindle, Centriole, QH301-705.5, Science, Mitosis, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Bioinformatics, General Biochemistry, Genetics and Molecular Biology, Spindle pole body, 03 medical and health sciences, 0302 clinical medicine, Microtubule, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Biology (General), [SDV.BDD]Life Sciences [q-bio]/Development Biology, Cytoskeleton, 030304 developmental biology, 0303 health sciences, Kinetochore, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Anastral, Chromatin, Cell biology, Spindle checkpoint, Centrosome, Cell lines, Drosophila, General Agricultural and Biological Sciences, 030217 neurology & neurosurgery, Research Article
Abstract: Summary In animal cells the centrosome is commonly viewed as the main cellular structure driving microtubule (MT) assembly into the mitotic spindle apparatus. However, additional pathways, such as those mediated by chromatin and augmin, are involved in the establishment of functional spindles. The molecular mechanisms involved in these pathways remain poorly understood, mostly due to limitations inherent to current experimental systems available. To overcome these limitations we have developed six new Drosophila cell lines derived from Drosophila homozygous mutants for DSas-4, a protein essential for centriole biogenesis. These cells lack detectable centrosomal structures, astral MT, with dispersed pericentriolar proteins D-PLP, Centrosomin and γ-tubulin. They show poorly focused spindle poles that reach the plasma membrane. Despite being compromised for functional centrosome, these cells could successfully undergo mitosis. Live-cell imaging analysis of acentriolar spindle assembly revealed that nascent MTs are nucleated from multiple points in the vicinity of chromosomes. These nascent MTs then grow away from kinetochores allowing the expansion of fibers that will be part of the future acentriolar spindle. MT repolymerization assays illustrate that acentriolar spindle assembly occurs “inside-out” from the chromosomes. Colchicine-mediated depolymerization of MTs further revealed the presence of a functional Spindle Assembly Checkpoint (SAC) in the acentriolar cells. Finally, pilot RNAi experiments open the potential use of these cell lines for the molecular dissection of anastral pathways in spindle and centrosome assembly.
Published: 2013
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42. Data publication with the structural biology data grid supports live analysis

Author: Pa, Meyer, Socias S, Key J, Ransey E, Ec, Tjon, Alejandro Buschiazzo, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Ks, Anderson, Rh, Baxter, Sc, Blacklow, Tj, Boggon, Am, Bonvin, Borek D, Tj, Brett, Caflisch A, Ci, Chang, Department of Biological Chemistry and Molecular Pharmacology [Boston] (DBCMP), Harvard Medical School [Boston] (HMS), Molecular and structural microbiology = Microbiología Molecular y Estructural [Montevideo], Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Shanghai Institutes for Biological Sciences (Institute of Biochemistry and Cell Biology), Chinese Academy of Sciences [Beijing] (CAS), NE-CAT and department of chemistry and chemical biology [Argonne], Cornell University, Departments of pharmacology and molecular biophysics and biochemistry [New Haven], Yale University School of Medicine, Department of Chemistry and Molecular Biophysics & Biochemistry [New Haven], Yale University [New Haven], Bijovet center [Utrecht], Utrecht University [Utrecht], Department of Biophysics and Biochemistry [Dallas], University of Texas Southwestern Medical Center [Dallas], Department of Internal Medicine [St Louis], Washington University School of Medicine, Department of Biochemistry [Zurich], University of Zürich [Zürich] (UZH), Institute of Biological Chemistry (IBC Sinica), Academia Sinica, Departments of biochemistry and chemistry [Nashville], Vanderbilt University [Nashville], Ludwig Institute for Cancer Research, University of California [San Diego] (UC San Diego), University of California, SUNY Upstate Medical University, State University of New York (SUNY), University of Chicago, Dana-Farber Cancer Institute [Boston], Saint Louis University School of Medicine [St Louis], Massachusetts Institute of Technology (MIT), Department of Biological Chemistry and Molecular Pharmacology (DBCMP), Columbia University [New York], National Heart, Lung, and Blood Institute [Bethesda] (NHLBI), Weill Institute for Cell and Molecular Biology, Howard Hughes Medical Institute [Stanford], Stanford University School of Medicine [CA, USA], Harvard University [Cambridge], Wuhan Institute of Virology, Howard Hughes Medical Institute [Boston], Tufts University School of Medicine [Boston], Queen's University [Kingston], La Trobe University [Melbourne], Geisel School of Medicine at Dartmouth, University of Cambridge [UK] (CAM), University of Texas at Dallas [Richardson] (UT Dallas), Campbell Family Institute for Breast Cancer Research, University Health Network, University of Toronto, Instituto de Biologia Molecular e Celular (IBMC), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), University of Maryland [Baltimore County] (UMBC), University of Maryland System, National Institute of Neurological Disorders and Stroke [Bethesda] (NINDS), National Institutes of Health [Bethesda] (NIH), Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch (UTMB), Rice University [Houston], University of Washington School of Medicine, University of Kentucky, Boston Children's Hospital, Department of Computer Science, University of Chicago, University of California [San Francisco] (UCSF), Uppsala University, Lawrence Berkeley National Laboratory [Berkeley] (LBNL), Howard Hughes Medical Institute [Ashburn], Fachbereich Biologie, University of Konstanz, Institute for Quantitative Social Sciences, Development of the Structural Biology Data Grid is funded by The Leona M. and Harry B. Helmsley Charitable Trust 2016PG-BRI002 to PS and MC. Development of citation workflows is supported NSF 1448069 (to PS). DAA is being developed as a pilot project of the National Data Service, with additional funds to support storage and technology development, including NIH P41 GM103403 (NE-CAT) and 1S10RR028832 (HMS) and DOE DE-AC02-06CH11357, NIH 1U54EB020406-01, Big Data for Discovery Science Center, and NIST 60NANB15D077 (Globus Project), Department of Biological Chemistry and Molecular Pharmacology [Boston] ( DBCMP ), Harvard Medical School [Boston] ( HMS ), Laboratory of molecular and structural microbiology, Institut Pasteur Montevideo-Réseau International des Instituts Pasteur ( RIIP ), Shanghai Institutes for Biological Sciences ( Institute of Biochemistry and Cell Biology ), Chinese Academy of Sciences [Beijing] ( CAS ), Yale University, Faculty of Science, Utrecht University, University of Zürich [Zürich] ( UZH ), Institute of Biological Chemistry ( IBC Sinica ), Vanderbilt University of Nashville, University of California [San Diego] ( UC San Diego ), Massachusetts Institute of Technology ( MIT ), Department of Biological Chemistry and Molecular Pharmacology ( DBCMP ), National Heart, Lung, and Blood Institute [Bethesda] ( NHLBI ), Stanford University School of Medicine, Havard Medical School, University of Cambridge [UK] ( CAM ), University of Texas at Dallas [Richardson] ( UT Dallas ), Instituto de Biologia Molecular e Celular ( IBMC ), Institut de biologie structurale ( IBS - UMR 5075 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), University of Maryland Baltimore County [Baltimore] ( UMBC ), National Institute of Neurological Disorders and Stroke [Bethesda] ( NINDS ), National Institutes of Health [Bethesda] ( NIH ), The University of Texas Medical Branch ( UTMB ), Yale School of Medicine, University of California [San Francisco] ( UCSF ), Lawrence Berkeley National Laboratory [Berkeley] ( LBNL ), Massachusetts Institute of Technology. Department of Biology, Massachusetts Institute of Technology. Department of Chemistry, Drennan, Catherine L., Schwartz, Thomas, Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS), and Instituto de Investigação e Inovação em Saúde
Subjects: Data set, Data quality, General Physics and Astronomy, Experimental data, General Chemistry, Data grid, General Biochemistry, Genetics and Molecular Biology, Data science, Genetics, Paradigm shift, Data access, Data mining, Biology, The Internet
Abstract: The validation and analysis of X-ray crystallographic data is essential for reproducibility and the development of crystallographic methods. Here, the authors describe a repository for crystallographic datasets and demonstrate some of the ways it could serve the crystallographic community., Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models and indispensable for development of structural biology processing methods. Here, we established a diffraction data publication and dissemination system, Structural Biology Data Grid (SBDG; data.sbgrid.org), to preserve primary experimental data sets that support scientific publications. Data sets are accessible to researchers through a community driven data grid, which facilitates global data access. Our analysis of a pilot collection of crystallographic data sets demonstrates that the information archived by SBDG is sufficient to reprocess data to statistics that meet or exceed the quality of the original published structures. SBDG has extended its services to the entire community and is used to develop support for other types of biomedical data sets. It is anticipated that access to the experimental data sets will enhance the paradigm shift in the community towards a much more dynamic body of continuously improving data analysis.
Published: 2016
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43. NF-κB pathway controls mitochondrial dynamics

Author: Laforge , M, Rodrigues , V, Silvestre , R, Gautier , Claude, Weil , Robert, Corti , O, Estaquier , J, Fédération de Recherche en Neurosciences (FR 3636), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Parasite Disease Group, Universidade do Porto = University of Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Life and Health Sciences Research Institute [Braga] (ICVS), University of Minho [Braga], Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Signalisation Moléculaire et Activation Cellulaire (SMAC), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Infectiologie, CHU de Québec, This work was funded by grants to JE from the Agence Nationale de Recherches sur le Sida et les Hépatites Virales (ANRS), the Association pour la Recherche sur le Cancer (ARC), La Ligue contre le Cancer (Ligue), and the Fondation pour la Recherche Médicale, OC is supported by the Fondation ICM, ‘Investissements d’avenir’ ANR-10-IAIHU-06. ML is supported by fellowships from the ANRS. VR is supported by a fellowship from FCT code SFRH/BD/64064/2009, ANR-10-IAHU-0006,IHU-A-ICM,Institut de Neurosciences Translationnelles de Paris(2010), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Universidade do Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), CNRS FR 3636, Université Paris Descartes, Paris, France. (Fédération des Neurosciences), Universidade do Minho, HAL-UPMC, Gestionnaire, Institut de Neurosciences Translationnelles de Paris - - IHU-A-ICM2010 - ANR-10-IAHU-0006 - IAHU - VALID, Fédération de Recherche en Neurosciences ( FR 3636 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Universidade do Porto [Porto]-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Life and Health Sciences Research Institute [Braga] ( ICVS ), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute ( ICM ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Pitié-Salpêtrière [APHP]-Centre National de la Recherche Scientifique ( CNRS ), Signalisation Moléculaire et Activation Cellulaire ( SMAC ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), and program 'Investissements d'avenir' ANR-10-IAIHU-06,program 'Investissements d'avenir' ANR-10-IAIHU-06
Subjects: Original Paper, endocrine system, Science & Technology, [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology, [SDV]Life Sciences [q-bio], Ubiquitin-Protein Ligases, Intracellular Signaling Peptides and Proteins, NF-kappa B, Gene Expression Regulation, Developmental, Apoptosis, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Fibroblasts, eye diseases, Cell Line, GTP Phosphohydrolases, I-kappa B Kinase, Mitochondria, Mice, Animals, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, Signal Transduction, bcl-2-Associated X Protein
Abstract: The Optic atrophy 1 protein (OPA1) is a key element in the dynamics and morphology of mitochondria. We demonstrated that the absence of I?B kinase-a, which is a key element of the nonclassical NF-?B pathway, has an impact on the mitochondrial network morphology and OPA1 expression. In contrast, the absence of NF-?B essential modulator (NEMO) or I?B kinase-ß, both of which are essential for the canonical NF-?B pathway, has no impact on mitochondrial dynamics. Whereas Parkin has been reported to positively regulate the expression of OPA1 through NEMO, herein we found that PARK2 overexpression did not modify the expression of OPA1. PARK2 expression reduced the levels of Bax, and it prevented stress-induced cell death only in Bak-deficient mouse embryonic fibroblast cells. Collectively, our results point out a role of the nonclassical NF-?B pathway in the regulation of mitochondrial dynamics and OPA1 expression., This work was funded by grants to JE from the Agence Nationale de Recherches sur le Sida et les Hepatites Virales (ANRS), the Association pour la Recherche sur le Cancer (ARC), La Ligue contre le Cancer (Ligue), and the Fondation pour la Recherche Medicale; OC is supported by the Fondation ICM, 'Investissements d'avenir' ANR-10-IAIHU-06. ML is supported by fellowships from the ANRS. VR is supported by a fellowship from FCT code SFRH/BD/64064/2009. We thank C Soundaramourty for her help, and V Baud who kindly provided the I kappa B kinase-alpha-deficient MEF cells IKK beta-/-, and IKK alpha beta-/-. MEF cells were provided by Inder M. Verma, and NEMO MEFs cells were a gift from Marc Schmidt-Supprian. JE acknowledges the support of the Canada Research Chair program.
Published: 2016
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44. Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus

Author: Vicente Pallás, María Carmen Herranz, Nicola Fiore, J. Montealegre, Jesús A. Sánchez-Navarro, S. Prodan, Frederic Aparicio, Thor Vinícius Martins Fajardo, Santiago F. Elena, NICOLA FIORE, Universidade de Chile, THOR VINICIUS MARTINS FAJARDO, CNPUV, SIMONA PRODAN, Universidade de Chile, MARÍA CARMEN HERRANZ, Instituto de Biologia Molecular y Celular de Plantas-Espanha, FREDERIC APARICIO, Instituto de Biologia Molecular y Celular de Plantas-Espanha, JAIME MONTEALEGRE, Universidade de Chile, SANTIAGO F. ELENA, Instituto de Biologia Molecular y Celular de Plantas-Espanha, VICENTE PALLÁS, Instituto de Biologia Molecular y Celular de Plantas-Espanha, and JÉSUS SÁNCHEZ-NAVARRO, Instituto de Biología Molecular y Celular de Plantas-Espanha.
Subjects: Nonsynonymous substitution, Genes, Viral, Molecular Sequence Data, Ilarvirus, Biology, Conserved sequence, Prunus, Ameixa, Virology, Genetic variation, Amino Acid Sequence, Cloning, Molecular, Selection, Genetic, Conserved Sequence, Phylogeny, DNA Primers, Diversidade genética, Genetics, Base Sequence, Sequence Homology, Amino Acid, Phylogenetic tree, Vírus, Genetic Variation, Doença de Planta, General Medicine, South America, biology.organism_classification, Genética, Plant Viral Movement Proteins, Fruticultura, Alfalfa mosaic virus, Prunus necrotic ringspot virus, Pêssego, RNA, Viral, Capsid Proteins
Abstract: Prunus necrotic ringspot virus (PNRSV) is distributed worldwide, but no molecular data have been previously reported from South American isolates. The nucleotide sequences corresponding to the movement (MP) and coat (CP) proteins of 23 isolates of PNRSV from Chile, Brazil, and Uruguay, and from different Prunus species, have been obtained. Phylogenetic analysis performed with full-length MP and CP sequences from all the PNRSV isolates confirmed the clustering of the isolates into the previously reported PV32-I, PV96-II and PE5-III phylogroups. No association was found between specific sequences and host, geographic origin or symptomatology. Comparative analysis showed that both MP and CP have phylogroup-specific amino acids and all of the motifs previously characterized for both proteins. The study of the distribution of synonymous and nonsynonymous changes along both open reading frames revealed that most amino acid sites are under the effect of negative purifying selection.
Published: 2008
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45. Comparative Proteomic Profiling of Ehrlichia ruminantium Pathogenic Strain and Its High-Passaged Attenuated Strain Reveals Virulence and Attenuation-Associated Proteins

Author: Elisabete Pires, Markus Müller, Miguel Ventosa, Thierry Lefrançois, Ana Varela Coelho, Nathalie Vachiery, Isabel Marcelino, Frédérique Lisacek, Instituto de Biologia Experimental e Tecnológica, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (NOVA), Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Proteome Informatics, Swiss Institute of Bioinformatics, EU projects COST action [FA-1002], EPIGENESIS RegPot European project [315988], Instituto de Biologia Experimental e Tecnológica (IBET), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Swiss Institute of Bioinformatics [Lausanne] (SIB), and Université de Lausanne (UNIL)-Université de Lausanne (UNIL)
Subjects: Proteomics, tique, Glycosylation, Proteome, Biodiversité et Ecologie, Rickettsiales, lcsh:Medicine, cowdria, L73 - Maladies des animaux, Protéine microbienne, biodiversité, Pathogénèse, chemistry.chemical_compound, Caprin, Ehrlichia ruminantium, lcsh:Science, protéome, Chèvre, 0303 health sciences, Multidisciplinary, Virulence, analyse statistique, santé animale, Effector, 3. Good health, protéine, Research Article, virulent strain, Hypovirulence, Virulence Factors, Ehrlichia, Biology, Microbiology, Biodiversity and Ecology, 03 medical and health sciences, statistical analysis, Bacterial Proteins, maldi tof, Électrophorèse sur gel, Maladie transmissible par tiques, 030304 developmental biology, Génie génétique, animal health, 030306 microbiology, Proteomic Profiling, Intracellular parasite, lcsh:R, électrophorèse sds page, biology.organism_classification, souche virulente, chemistry, lcsh:Q, U30 - Méthodes de recherche, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, protein, Protein Processing, Post-Translational
Abstract: The obligate intracellular bacterium Ehrlichia ruminantium (ER) causes heartwater, a fatal tick-borne disease in livestock. In the field, ER strains present different levels of virulence, limiting vaccine efficacy, for which the molecular basis remains unknown. Moreover, there are no genetic tools currently available for ER manipulation, thus limiting the knowledge of the genes/proteins that are essential for ER pathogenesis and biology. As such, to identify proteins and/or mechanisms involved in ER virulence, we performed the first exhaustive comparative proteomic analysis between a virulent strain (ERGvir) and its high-passaged attenuated strain (ERGatt). Despite their different behaviors in vivo and in vitro, our results from 1DE-nanoLC-MS/MS showed that ERGvir and ERGatt share 80% of their proteins; this core proteome includes chaperones, proteins involved in metabolism, protein-DNA-RNA biosynthesis and processing, and bacterial effectors. Conventional 2DE revealed that 85% of the identified proteins are proteoforms, suggesting that post-translational modifications (namely glycosylation) are important in ER biology. Strain-specific proteins were also identified: while ERGatt has an increased number and overexpression of proteins involved in cell division, metabolism, transport and protein processing, ERGvir shows an overexpression of proteins and proteoforms (DIGE experiments) involved in pathogenesis such as Lpd, AnkA, VirB9 and B10, providing molecular evidence for its increased virulence in vivo and in vitro. Overall, our work reveals that ERGvir and ERGatt proteomes are streamlined to fulfill their biological function (maximum virulence for ERGvir and replicative capacity for ERGatt), and we provide both pioneering data and novel insights into the pathogenesis of this obligate intracellular bacterium.
Published: 2015
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46. Leishmania infantum Modulates Host Macrophage Mitochondrial Metabolism by Hijacking the SIRT1-AMPK Axis

Author: Moreira, Diana, Rodrigues, Vasco, Abengozar, Maria, Rivas, Luis, Rial, Eduardo, Laforge, Mireille, Li, Xiaoling, Foretz, Marc, Viollet, Benoit, Estaquier, Jérôme, Cordeiro Da Silva, Anabela, Silvestre, Ricardo, Parasite Disease Group, Universidade do Porto [Porto]-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Mort cellulaire programmée et Signalisation ( FR 3636 ), Fédération de Recherche en Neurosciences ( FR 3636 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Unidad Metabolómica, Interacciones y Bioanálisis ( UMIB ), Consejo Superior de Investigaciones Científicas [Spain] ( CSIC ) -Centro de Investigaciones Biológicas (CIB), Department of Cellular and Molecular Medicine, Consejo Superior de Investigaciones Científicas [Spain] ( CSIC ) - Centro de Investigaciones Biológicas (CIB), Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de Recherche du CHU de Québec, Université Laval, Departamento de Ciências Biológicas, Universidade do Porto [Porto], This work was funded by FEDER fundsthrough the Operational CompetitivenessProgramme - COMPETE and by National Fundsthrough FCT - Fundação para a Ciência e aTecnologia under the project FCOMP-01-0124-FEDER-011054 (PTDC/SAU-FCF/100749/2008) andPTDC/BIA-MIC/118644/2010. DM and VR were supported by SFRH/BD/91543/2012 and SFRH/BD/64064/2009,respectively. LR was supported by PN de I+D+I 2008-2011, PI12-02706 and VI PN de I+D+I 2008-2011,ISCIII -Subdirección General de Redes y Centros deInvestigación Cooperativa-FEDER (RICET RD12/0018/0007). ER was supported by a project grant ofthe Spanish Ministerio de Economía y Competitividad(SAF2010-20256). JE was supported by an ANRgrant (LEISH-APO, France) and a Partenariat HubertCurien (PHC) (program Volubilis, MA/11/262). JE isalso supported by the Canada Research Chairprogramme. ML was supported by a fellowship fromANR. RS was supported by Programa Ciência -financed by Programa Operacional PotencialHumano POPH - QREN - Tipologia 4.2 - Promocãodo Emprego Científico, co-funded by Fundo SocialEuropeu and National funding from Ministry ofScience, Technology and Higher Education(MCTES)., Instituto de Investigação e Inovação em Saúde, Universidade do Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Mort cellulaire programmée et Signalisation (FR 3636 ), Fédération de Recherche en Neurosciences (FR 3636), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unidad Metabolómica, Interacciones y Bioanálisis (UMIB), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)- Centro de Investigaciones Biológicas (CIB), National Institutes of Health [Bethesda] (NIH), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Laval [Québec] (ULaval), Universidade do Porto, Bos, Mireille, Universidade do Porto = University of Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Universidade do Porto = University of Porto
Subjects: lcsh:Immunologic diseases. Allergy, Ubiquitination/physiology, AMP-Activated Protein Kinases, Protein Serine-Threonine Kinases, Oxidative Phosphorylation, Protein Transport/physiology, Mice, Sirtuin 1, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Leishmania infantum, lcsh:QH301-705.5, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Immune Evasion, Mice, Knockout, Mice, Inbred BALB C, Glucose Transporter Type 4, Signal Transduction/physiology, Peroxisomes/metabolism, Macrophages, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Mitochondria, Peroxisome-Targeting Signal 1 Receptor/metabolism, lcsh:Biology (General), Leishmaniasis, Visceral, lcsh:RC581-607, Peroxisomal Targeting Signal 2 Receptor/metabolism, Transcription Factors, Research Article
Abstract: Metabolic manipulation of host cells by intracellular pathogens is currently recognized to play an important role in the pathology of infection. Nevertheless, little information is available regarding mitochondrial energy metabolism in Leishmania infected macrophages. Here, we demonstrate that during L. infantum infection, macrophages switch from an early glycolytic metabolism to an oxidative phosphorylation, and this metabolic deviation requires SIRT1 and LKB1/AMPK. SIRT1 or LBK1 deficient macrophages infected with L. infantum failed to activate AMPK and up-regulate its targets such as Slc2a4 and Ppargc1a, which are essential for parasite growth. As a result, impairment of metabolic switch caused by SIRT1 or AMPK deficiency reduces parasite load in vitro and in vivo. Overall, our work demonstrates the importance of SIRT1 and AMPK energetic sensors for parasite intracellular survival and proliferation, highlighting the modulation of these proteins as potential therapeutic targets for the treatment of leishmaniasis., Author Summary Leishmania infantum, a causative agent of visceral leishmaniasis, is able to infect host macrophages and modulate a myriad of signalling pathways that contributes to the disease outcome. In order to survive, L. infantum must compete with the host for the same metabolic resources, however scarce attention has been dedicated to clarify the potential interference of the parasite with the host metabolic pathways and its impact for the infection outcome. We analysed the macrophage metabolic alterations induced by L. infantum focusing on host energetic players exploited by the parasite. We describe that L. infantum induced a metabolic switch from an early aerobic glycolytic environment to a later mitochondrial metabolism. In this process, L. infantum modulates important energetic sensors of the host, such as the SIRT1-LKB1-AMPK axis. This triad is important for the recovery of the host energetic status and also for the parasite survival. With this work, we demonstrate that the host SIRT1-LKB1-AMPK axis has a crucial impact on the parasite survival in vitro and in vivo.
Published: 2015
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47. Comprehensive Genome-Wide Analysis of the Aux/IAA Gene Family in Eucalyptus: Evidence for the Role of EgrIAA4 in Wood Formation

Author: Marçal Soler, Mondher Bouzayen, Jacqueline Grima-Pettenati, Isabelle Mila, Hélène San Clemente, Jorge A. P. Paiva, Hua Cassan-Wang, Alexander Andrew Myburg, Hong Yu, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Instituto de Biologia Experimental e Tecnológica - iBET (PORTUGAL), University of Pretoria (SOUTH AFRICA), Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Instituto de Investigaçao Cientifica Tropical, Instituto de Biologia Experimental e Tecnológica (IBET), University of Pretoria [South Africa], Régulation et Dynamique de la Formation du Bois, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Centre National pour la Recherche Scientifique (CNRS), University Paul Sabatier Toulouse III (UPS), Agence Nationale de la Recherche (ANR), French Laboratory of Excellence (ANR-10-LABX-41, ANR-11-IDEX-0002-02), Plant KBBE TreeForJoules (ANR-2010-KBBE-007-01, P-KBBE/AGR_GPL/0001/2010), project microEGo (PTDC/AGR-GPL/098179/2008), China Scholarship Council, Departament d'Universitats, Recerca i Societat de la Informacio de la Generalitat de Catalunya, Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BPD/92207/2013], Institut National Polytechnique de Toulouse - INPT (FRANCE), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Toulouse-Institut National Polytechnique (Toulouse) (Toulouse INP)
Subjects: Transcription, Genetic, Physiology, Arabidopsis, Plant Science, Aux/IAA, Secondary cell walls, Gene Expression Regulation, Plant, Génétique des plantes, Auxin, Phylogeny, Plant Proteins, chemistry.chemical_classification, Eucalyptus, food and beverages, Cell Differentiation, General Medicine, Plants, Genetically Modified, Wood, Cell biology, Protein Transport, Phenotype, Organ Specificity, Multigene Family, Genome, Plant, Subcellular Fractions, Plant Development, Biology, Environment, Genes, Plant, Chromosomes, Plant, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, Gravitropism, Species Specificity, Xylem, Botany, Vascular cambium, Gene family, Cambium, Genetic Association Studies, Cell Nucleus, Wood formation, Indoleacetic Acids, Gene Expression Profiling, Lateral root, fungi, Cell Biology, Sequence Analysis, DNA, 15. Life on land, biology.organism_classification, Gene expression profiling, chemistry, Gene expression
Abstract: International audience; Auxin plays a pivotal role in various plant growth and development processes, including vascular differentiation.The modulation of auxin responsiveness through the auxin perception and signaling machinery is believed to be a major regulatory mechanism controlling cambium activity and wood formation. To gain more insights into the rolesof key Aux/IAA gene regulators of the auxin response in these processes, we identified and characterized membersof the Aux/IAA family in the genome of Eucalyptus grandis, a tree of worldwide economic importance. We found that the gene family in Eucalyptus is slightly smaller than that in Populus and Arabidopsis, but all phylogenetic groupsare represented. High-throughput expression profiling of different organs and tissues highlighted several Aux/IAAgenes expressed in vascular cambium and/or developing xylem, some showing differential expression in response todevelopmental (juvenile vs. mature) and/or to environmental (tension stress) cues. Based on the expression profiles,we selected a promising candidate gene, EgrIAA4, for functional characterization. We showed that EgrIAA4 protein is localized in the nucleus and functions as an auxin-responsive repressor. Overexpressing a stabilized version of EgrIAA4 in Arabidopsis dramatically impeded plant growth and fertility and induced auxin-insensitive phenotypes such as inhibition of primary root elongation, lateral root emergence and agravitropism. Interestingly, the lignified secondary walls of the interfascicular fibers appeared very late, whereas those of the xylary fibers were virtually undetectable, suggesting that EgrIAA4 may play crucial roles in fiber development and secondary cell wall deposition.
Published: 2015
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48. Interleukin-13 in the skin and interferon-gamma in the liver are key players in immune protection in human schistosomiasis

Author: Virmondes Rodrigues, Nasr Eldin Elwali, Yuesheng Li, Ogobara K. Doumbo, Laurent Argiro, Christophe Chevillard, Bourema Kouriba, Alain Dessein, Carole Eboumbou, Sandrine Marquet, Hélia Dessein, Génétique et immunologie des maladies parasitaires (GIMP), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Parasite Disease Group, Universidade do Porto [Porto]-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Fédération de Recherche en Neurosciences (FR 3636), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Malaria Research and Training Center (MRTC), Faculté de Médecine de Bamako, Université de Douala, Immunologie et Génétique des Maladies Parasitaires [Aix Marseille Université] (IGMP - U399 Inserm - AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de parasitologie-mycologie [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Laboratory of Immunology, Universidade Federal do Triângulo Mineiro (UFTM), Hunan Institute of Parasitic Diseases, Universidade do Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Universidade do Porto = University of Porto-Instituto de Biologia Molecular e Celular (IBMC)-Instituto de Investigação e Inovação em Saúde, Génétique et immunologie des maladies parasitaires ( GIMP ), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Fédération de Recherche en Neurosciences ( FR 3636 ), and Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: [SDV]Life Sciences [q-bio], Immunology, Locus (genetics), Biology, Interferon-gamma, 03 medical and health sciences, 0302 clinical medicine, Immune system, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, Interferon, Immunity, medicine, Animals, Humans, Schistosomiasis, Immunology and Allergy, Interferon gamma, ComputingMilieux_MISCELLANEOUS, Skin, 030304 developmental biology, 0303 health sciences, Interleukin-13, Interleukin, biology.organism_classification, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Liver, Interleukin 13, Schistosoma, Schistosoma mansoni, 030215 immunology, medicine.drug
Abstract: Immunity against schistosomes includes anti-infection immunity, which is mainly active against invading larvae in the skin, and anti-disease immunity, which controls abnormal fibrosis in tissues invaded by schistosome eggs. Anti-infection immunity is T-helper 2 (Th2) cell-dependent and is controlled by a major genetic locus that is located near the Th2 cytokine locus on chromosome 5q31-q33. Mutations in the gene encoding interleukin (IL)-13 that decrease or increase IL-13 production account, at least in part, for that genetic control. In contrast, protection against hepatic fibrosis is dependent on interferon (IFN)-gamma and is controlled by a major genetic locus that is located on 6q23, near the gene encoding the IFN-gamma receptor beta chain. Mutations that modulate IFN-gamma gene transcription are associated with different susceptibility to disease. These data indicate that IL-13 in the skin and IFN-gamma in the liver are key players in protective immunity against schistosomes. These roles relate to the high anti-fibrogenic activities of IFN-gamma and to the unique ability of IL-13 in Th2 priming in the skin and in the mobilization of eosinophils in tissues. The coexistence of strong IFN-gamma and IL-13-mediated immune responses in the same subject may involve the compartmentalization of the anti-schistosome immune response between the skin and the liver.
Published: 2004
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49. Genomic prediction in a multi-generation Eucalyptus globulus breeding population

Author: Geoffrey Haristoy, Laurent Bouffier, Luis Fontes, Luis Leal, Jorge A. P. Paiva, João-Pedro Pina, Jean-Marc Gion, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Altri Florestal, Associaçao CECOLAB, Instituto de Biologia Experimental e Tecnológica (IBET), and European Project: 773383,CORDIS
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Genetics, Forestry, Horticulture, Molecular Biology, Eucalyptus globulus, Genomic selection, GBLUP, Progeny validation, Pedigree error, Breeding programme
Abstract: International audience; Genomic selection is a promising approach for reducing the length of the selection cycle in forest tree breeding. Its efficiency must be evaluated across generations for this purpose, but such studies have been performed for multi-generational breeding programmes in only a few forest tree species to date. We analysed a subset of the Eucalyptus globulus breeding population from the Portuguese company Altri Florestal. In total, 412 genotypes from three successive breeding generations were genotyped with 14,716 SNP markers. A comparison of pedigree-based and marker-based relationship coefficients allowed to correct several documented pedigree errors. Deregressed breeding values were estimated from phenotypic records for growth traits (height and diameter) and survival for 31 field trials distributed in one breeding zone in Portugal and used as pseudo-phenotypes for genomic prediction models. Accuracy was assessed by cross-validation according to two main scenarios: (i) a scenario based on a five random fold number, not taking generation into account, and (ii) scenarios investigating progeny validation using parental generations to predict the progenies. Accuracy was highest after pedigree correction and ranged from 0.46 to 0.60 for the first scenario, from - 0.56 to 0.72 for parent/progeny scenarios and from 0.33 to 0.79 when progenies were added to the calibration population. This genomic selection study provides promising insight for the Altri Florestal Eucalyptus breeding programme.
Published: 2023
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50. Genome-Wide Characterization and Expression Profiling of the AUXIN RESPONSE FACTOR (ARF) Gene Family in Eucalyptus grandis

Author: Mondher Bouzayen, Bruno Savelli, Christophe Dunand, Hua Cassan-Wang, Hong Yu, Isabelle Mila, Marçal Soler, Alexander Andrew Myburg, Jorge A. P. Paiva, Jacqueline Grima-Pettenati, Hélène San Clemente, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Instituto de Biologia Experimental e Tecnológica - iBET (PORTUGAL), Instituto de Investigação Científica Tropical - IICT (PORTUGAL), University of Pretoria (SOUTH AFRICA), Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Dynamique et Evolution des Parois cellulaires végétales, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Instituto de Investigaçao Cientifica Tropical, Instituto de Biologia Experimental e Tecnológica (IBET), University of Pretoria [South Africa], Régulation et Dynamique de la Formation du Bois, The Centre National pour la Recherche Scientifique (CNRS), the University Paul Sabatier Toulouse III (UPS), the French Laboratory of Excellence project ‘‘TULIP’’ (ANR-10-LABX-41, ANR-11-IDEX-0002-02) and the Plant KBBE TreeForJoules project (ANR-2010-KBBE-007-01 (FR) and PKBBE/AGR_GPL/0001/2010 (FCT, PT)), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Toulouse-Institut National Polytechnique (Toulouse) (Toulouse INP), and Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Subjects: Plant Phylogenetics, Plant Evolution, cambial region, woody plant, plant hormone auxin, organogenesis, Organogenesis, Arabidopsis, Plant Science, Plant Genetics, Plant hormone auxin, Biochemistry, Genome, Gene Duplication, Gene duplication, Génétique des plantes, Plant Hormones, Phylogeny, Plant Proteins, Genetics, Eucalyptus, Agroforests, Multidisciplinary, Plant Biochemistry, Protoplasts, Chromosome Mapping, Gene Expression Regulation, Developmental, food and beverages, Agriculture, Forestry, Phylogenetic Analysis, Genomics, Phylogenetics, Multigene Family, Medicine, Genome, Plant, Research Article, Science, Plants genetics, Biology, Molecular Evolution, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, Cambial region, Tobacco, Genome-Wide Association Studies, Vascular cambium, Gene family, Evolutionary Systematics, Gene Prediction, Molecular Biology Techniques, Woody plant, Molecular Biology, Gene, Crop Genetics, Evolutionary Biology, Molecular Biology Assays and Analysis Techniques, Gene Expression Profiling, fungi, Biology and Life Sciences, Computational Biology, Promoter, 15. Life on land, Genome Analysis, Hormones, Organismal Evolution, Gene expression profiling, Auxins, Gene Function, Transcriptome, Agroecology, Transcription Factors
Abstract: International audience; Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF) are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation.
Published: 2014
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