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Airborne nanoplastics can enter alveolar cells and trigger intracellular oxidative stress primarily. Herein, taking advantage of the high electrochemical resolution of SiC@Pt nanoelectrodes, we achieved the quantitative discrimination of the major ROS/RNS within A549 cells, disclosed the sources of their precursors, and observed that the NO (RNS precursor) level significantly increased, whereas O2˙− (ROS precursor) remained relatively stable during the nanoplastics exposure. This establishes that iNOS or mitochondrion-targeted treatment may be a preventive or therapeutic strategy for nanoplastic-induced lung injury. [ABSTRACT FROM AUTHOR]

[Display omitted] • The PEI-Im-CTG CEMs were modified via Debus-Radziszewski in-situ crosslinking. • The PEI-Im layer had rigid structure and strong cation-surface interaction. • The PEI-Im-CTG membrane exhibited high permselectivity between Li+ and Mg2+. • The PEI-Im-CTG membrane showed a high Li+ purity during two-stage ED process. Lithium extraction from salt-lakes is a crucial way to solve the global shortage of lithium resources. With high sustainability and efficient ion separation, electrodialysis (ED) has a high potential for lithium extraction from salt-lakes, yet suffering from the challenges of cation-cation selectivity. In this work, a facile Debus-Radziszewski in-situ cross-linking approach was proposed to construct a polyethyleneimine modification layer containing imidazole groups (PEI-Im) on a common cation exchange membrane (commercial membrane, i.e., CTG membrane) surface to enhance the lithium selectivity. The pore size, architecture rigidity and charged characteristic of this PEI-Im layer were controlled via adjusting the reaction conditions. The resultant membrane (PEI-Im-CTG) exhibited high Li+ permeation rate (0.89 mol·m−2·h−1) and excellent Li+/Mg2+ permselectivity (13.1), which are 4.0 times and 19.8 times higher than that of the pristine CTG membrane, respectively. Moreover, the PEI-Im-CTG membrane based two-stage ED process showed a high Li+ purity (∼99.90 %) and low energy consumption (∼0.158 kWh·mol-1Li+) for Li+ extraction from simulated salt-lakes. This work offers a facile and effective strategy for membrane modification with high Li+ selectivity, which is promising in lithium extraction from salt-lakes. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate effects of Funneliformis mosseae on plant growth and root exudate compositions and contents, soil and root phosphatase activity, soil glomalin concentrations, and thus soil aggregate stability and distribution in trifoliate orange under well-watered (75% of maximum water holding capacity) and drought stress (55% of maximum water holding capacity) conditions. After eight weeks of drought treatment, mycorrhizal fungal inoculation improved plant growth and exhibited altered composition of root exudates than non-inoculated treatment. Mycorrhizal fungal inoculation dramatically increased the relative abundance of phenolics (e.g., 2 H,8 H-Benzo[1,2-b:3,4-b'] dipyran-2-one,8,8-dimethyl), terpenoids (e.g., geijerene), and acids (n-hexadecanoic acid), while notably reduced the relative abundance of alkanes (e.g., tridecane, 2-methyl-), esters (e.g., hexanedioic acid and dimethyl ester), and amides (e.g.,13-docosenamide) in root exudates. Mycorrhizal fungal colonization profoundly increased easily extractable and total glomalin-related soil protein levels under two soil water regimes, which cemented soil macroaggregate (2–4 mm size) formation, thereby, improving soil aggregate stability. Mycorrhizal fungal-inoculated plants represented higher soil acid, alkaline and total phosphatase activities, irrespective of well-watered and drought treatment. The results suggest that mycorrhizal plants had improved root microenvironment to mitigate drought damage through changes in root exudate components along with glomalin, phosphatase, and soil aggregate stability in the mycorrhizosphere. [ABSTRACT FROM AUTHOR]

Background: Direct moxibustion (DM) is reported to be useful for cervical spondylotic radiculopathy (CSR), but the analgesic mechanism remains unknown. Autophagy plays a protective role in neuronal apoptosis, Act A/Smads signaling pathway has been confirmed to be associated with the activation of autophagy. The study aimed to explore the effect of DM on autophagy in rats with CSR and the involvement of Act A/Smads signaling pathway. Methods: Rats were randomly divided into Sham, CSR, CSR + DM, CSR + DM + 3‐MA (PI3K inhibitor), and CSR + DM + SB (Act A inhibitor) group. Three days after establishment of CSR model with a fish line inserted under the axilla of the nerve roots, DM at Dazhui (GV14) was performed six times once for seven consecutive days. Western blot and immunofluorescence staining were used to observe the expression of the neuronal autophagy molecule LC3II/I, Atg7, and Act A/Smads signaling molecule Act A, p‐Smad2, and p‐Smad3. Bcl‐2/Bax mRNA expression was measured by real time PCR. Results: DM improved the pain threshold and motor function of CSR rats and promoted the expression of Act A, p‐Smad2, p‐Smad3, LC3II/I, and Atg7 in the entrapped‐nerve root spinal dorsal horn. DM reduced the expression of Bax mRNA and decreased the number of apoptotic neurons. 3‐MA and Act A inhibitor SB suppressed the expression of above‐mentioned proteins and reduced the protective effect of DM on apoptotic neurons. Conclusion: DM exerts analgesic effects by regulating the autophagy to reduce cell apoptosis and repair nerve injury, and this feature may be related to the Act A/Smads signaling pathway. [ABSTRACT FROM AUTHOR]

Titin, one of the largest proteins in humans, is a major component of muscle sarcomeres. Pathogenic variants in the titin gene (TTN) have been reported to cause a range of skeletal muscle diseases, collectively known as titinopathy. Titinopathy is a heterogeneous group of disabling diseases characterized by muscle weakness. In our study, we aimed to establish the clinicopathological‐genetic spectrum of titinopathy from a single neuromuscular center. Three patients were diagnosed as having definite titinopathy, and additional three patients were diagnosed as having possible titinopathy according to the diagnostic criteria. All the patients showed initial symptoms from age one to 40 years. Physical examination revealed that five patients had muscle weakness, and that one patient experienced behavioral changes. Muscle biopsy specimens obtained from all six patients demonstrated multiple myopathological changes, including increased fiber size variation, muscle fiber hypertrophy or atrophy, formation of centralized cell nuclei, necklace cytoplasmic bodies, and formation of rimmed vacuoles and cores. Genetic testing revealed 11 different TTN alterations, including missense (6/11), nonsense (2/11), frameshift (2/11), and splicing (1/11) mutations. Our study provides further evidence that TTN mutations are more likely to be responsible for an increasing proportion of various myopathies, such as hereditary myopathy with early respiratory failure (HMERF), core myopathy, and distal myopathy with rimmed vacuoles, than currently recognized mutations. Our findings expand the clinical, pathohistological and genetic spectrum of titinopathy. [ABSTRACT FROM AUTHOR]

Multiple Acyl‐CoA dehydrogenase deficiency (MADD), one of the most common lipid storage myopathies (LSMs), is a heterogeneous inherited muscular disorder that is pathologically characterized by numerous lipid droplets in muscle fibers due to lipid metabolism disturbance. MADD exhibits a wide range of clinical features, including skeletal muscle weakness and multisystem dysfunctions. However, MADD, as well as other types of LSM, associated with peripheral neuropathy has rarely been reported during the past four decades. Here, we present four Chinese patients affected by MADD with peripheral neuropathy in our neuromuscular center. Clinically, these four patients showed skeletal muscle weakness and prominent paresthesia. Muscle biopsy detected characteristic myopathological patterns of LSM, such as obvious lipid droplets in muscle fibers. Sural nerve biopsy revealed a severe reduction in number of myelinated nerve fibers, which is a typical neuropathological pattern of peripheral neuropathy. Causative ETFDH mutations were found in all four cases. The skeletal muscle weakness was rapidly improved after some treatments while paresthesia showed unsatisfactory improvement. The features of previously reported patients of this specific type are also summarized in this paper. We propose that MADD with peripheral neuropathy may be a new phenotypic subtype because the pathology and reaction to riboflavin treatment are different from those of traditional MADD, although further research on the precise pathogenesis and mechanisms is needed. [ABSTRACT FROM AUTHOR]

It is important to manage water resources of the upper Yellow River basin for the new Silk Road economic belt. In recent decades, under the combined human activities and influence of climate, the hydrologic regime of the upper Yellow River basin shows remarkable variations that have caused many issues. So potential human indicated influence has been drawing increasing attention from hydrologists and local governments. The aim of this study is to determine the changes in the hydrological characteristic parameters and mean annual runoff series of the upper Yellow River basin. This paper took the representative Lanzhou Station in the upstream Yellow River as an example, used the TFPW-MK mutation test and rank sum test to analyze the location of the variation points of hydrological series. By contrasting two different analysis results between natural and measured river runoff series, the impacts of human activities on the long-term hydrological regime were investigated. The variation range of hydrological ecological indexes before and after variation were analyzed by the method of indicators of hydrologic alteration (IHA). The results show: a) the hydrological series of Lanzhou Station is a significant decreasing trend can be observed in the natural stream flow series in 1985 with a high degree of hydrological variability, while human activities play an important role; b) various ecological indicators have changed in different degrees that have caused the deterioration of ecological conditions around 1985; and c) continuous decreasing stream flow in the upper Yellow River basin will trigger serious shortages of fresh water in the future, which may challenge the sustainability and safety of water resource development in the river basin, and should be paid great attention before 2020. Variation analysis and diagnosis of eco-hydrological indexes in the upper reaches of the Yellow River can provide a basis for the development, utilization, and protection of water resources in this area. [ABSTRACT FROM AUTHOR]

In order to resolve the situations of nonuniform coolant flow distribution and insufficient vortex suppression, the existing Pressurized Water Reactor (PWR) usually adopts complex coolant mixing structures. However, those structures will greatly increase the complexity and maintenance cost of the system. To solve this problem, a trimming-based design method is proposed in this paper for the complex system and applies it to the design process of the PWR coolant flow distribution device. The function model of the coolant flow distribution system is built based on its functional analysis, and, according to the result of the component feature analysis, the columns and part of the basket are trimmed in order to simplify the overall structure of the system. To further solve the technical contradictions occurred in the simplified system, the contradiction solving tools of TRIZ theory are adopted. By setting the stereo flow equalizing plate, which can strengthen the function of flow distribution and vortex suppression, a coolant flow distribution device for PWR based on dome structure is obtained finally. This device owns a simple structure with good effect on coolant flow distribution and vortex suppression, which can achieve the goal of uniform coolant flow distribution of the system effectively. [ABSTRACT FROM AUTHOR]

Energy-filtered transmission electron microscopy and electron tomography were applied to in situ studies of the formation, shape, and diameter of nanopores formed in a silicon nitride membrane in a transmission electron microscope. The nanopore geometry was observed in three dimensions by electron tomography. Drilling conditions, such as probe current, beam convergence angle, and probe position, affect the formation rate and the geometry of the pores. With a beam convergence semi-angle of α = 22 mrad, a conical shaped nanopore is formed but at α = 45 mrad, double-cone (hourglass-shaped) nanopores were produced. Nanopores with an effective diameter between 10 nm and 1.8 nm were fabricated by controlling the drilling time. [ABSTRACT FROM AUTHOR]

Polyamide (PA) thin film nanocomposite (TFN) reverse osmosis (RO) membranes have been vigorously developed, which could be used in saline water desalination, wastewater treatment and chemicals separation and purification. Aiming at further improving the TFN RO membrane comprehensive performances, such as the perm-selectivity, stability and anti-fouling/-microbial properties remains a great challenge. Herein, we proposed a biomimetic nanoparticle redox strategy to in-situ integration of multiple functional nanoparticles, i.e., biomimetic nanoparticles (BNPs) and silver (Ag) nanoparticles into the PA matrix for simultaneously regulating the membrane microstructure and optimizing the surface property. A much hydrophilic selective layer with interior transporting channel structure renders the resultant RO membrane PA-BNP-Ag with an enhanced water permeance of ∼4.2 L m−2·h−1bar−1 without sacrificing NaCl rejection (∼98.1%). The BNPs induce Ag nanoparticles uniformly distributed within the PA matrix, which significantly improves the membrane anti-fouling/-bacterial property. The water flux recovery ratio of PAM-BNP-Ag was improved to be higher than 96% during several cycles filtration of saline solution containing bovine serum albumin foulants. More importantly, the membrane sterilization rate against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) achieves nearly 100%. This work provides a new strategy for the construction of advanced PA TFN membranes with both high perm-selectivity and anti-fouling/-microbial property in RO desalination. [Display omitted] • The TFN RO membrane was fabricated with in-situ integration of multiple functional nanoparticles. • The TFN RO membrane with high hydrophilic surface and tunable microstructure. • The TFN RO membrane exhibits both high water permeance and NaCl rejection. • The TFN RO membrane represents excellent anti-fouling/-bacterial property. [ABSTRACT FROM AUTHOR]

For the first time, a preliminary foaming treatment for poly(vinyl chloride) (PVC) was adopted to facilitate the chlorination of PVC with the assistance of supercritical carbon dioxide and acetone. The chlorine content of chlorinated poly(vinyl chloride) (CPVC) obtained from pretreated PVC had exceeded 0.66 g g-1, while that of CPVC using nonpretreated PVC only reached about 0.60 g g-1. Analysis of the particle diameter and morphology of pretreated PVC showed that the preliminary treatment had enlarged the particle diameter and inner pore structure. Gel permeation chromatography, differential scanning calorimetry, and 13C NMR analysis showed that the obtained CPVC had a narrow molecular weight distribution, excellent thermal properties, and uniform chlorine distribution. Furthermore, an improved kinetics model was proposed to indicate the crucial step of chlorination. The frequency factor was expressed as the ratio of the particle volume of the foamed PVC to that of the original PVC. Consequently, the model values of the chlorine content agreed well with the experimental values. [ABSTRACT FROM AUTHOR]

Obesity is characterized by aberrant fat accumulation. However, the intracellular signaling pathway that senses dietary fat and leads to fat storage remains elusive. Here, we have observed that the levels of histone deacetylase 6 (HDAC6) and the related family member HDAC10 are markedly reduced in adipose tissues of obese animals and humans. Mice with adipocyte-specific depletion of Hdac6 exhibited increased fat accumulation and reduced insulin sensitivity. In normal adipocytes, we found that reversal of P300/CBP-associated factor-induced (PCAF-induced) acetylation at K56 on cell death-inducing DFFA-like effector C (CIDEC, also known as FSP27) critically regulated lipid droplet fusion and lipid storage. Importantly, HDAC6 deacetylates CIDEC, leading to destabilization and reduced lipid droplet fusion. Accordingly, we observed elevated levels of CIDEC and its acetylated form in HDAC-deficient adipocytes as well as the adipose tissue of obese animals and humans. Fatty acids (FAs) prevented CIDEC deacetylation by promoting the dissociation of CIDEC from HDAC6, which resulted in increased association of CIDEC with PCAF on the endoplasmic reticulum. Control of CIDEC acetylation required the conversion of FAs to triacylglycerols. Thus, we have revealed a signaling axis that is involved in the coordination of nutrient availability, protein acetylation, and cellular lipid metabolic responses. [ABSTRACT FROM AUTHOR]

In this paper, an approach to the design of robust observer for linear discrete-time periodic systems is proposed. By utilizing some algebraic techniques, a robustness index is deduced. On the robustness, the needed computation mainly consists in designing parameterized observers for this type of systems and solving an optimization problem. The proposed approach is finally illustrated by a simulation example. [ABSTRACT FROM AUTHOR]

A highly efficient asymmetric synthesis of dihydronaphthalenes is disclosed. The process represents a new addition to the limited asymmetric reactions of isobenzopyryliums, a family of versatile 10π-electron aromatic species. Excellent asymmetric induction is achieved for the first time without an anchoring group in the 4-position or a metal catalyst, both of which were required previously in these reactions. The success is attributed to the unusual chiral counteranion (meanwhile also the nucleophile) generated in situ from the chiral phosphate and the boronic acid as well as the leaving group. Preliminary control experiments provided important insight into the reaction mechanism. [ABSTRACT FROM AUTHOR]

In this paper, an approach to the design of robust observer for linear discrete-time periodic systems is proposed. By utilizing some algebraic techniques, a robustness index is deduced. On the robustness, the needed computation mainly consists in designing parameterized observers for this type of systems and solving an optimization problem. The proposed approach is finally illustrated by a simulation example. [ABSTRACT FROM AUTHOR]

Glutathione S -transferases (GSTs) are an essential multifunctional protein family with common detoxifying enzymes. In this study, 34 GST genes were identified from the melon fly, Zeugodacus cucurbitae , one of the most destructive pests worldwide. These GSTs include 32 cytosolic genes and two microsomal genes. Furthermore, these cytosolic GSTs were classified into six classes: 11 delta, 13 epsilon, three theta, one sigma, two zeta, and two omega. Most of these showed dynamic expression during the developmental stage, some of which showed stage-specific expression. The expression in various adult tissues showed that most of them were expressed in anti-stress-related tissues. The transcriptional response of the delta and epsilon families was determined when Z. cucurbitae was exposed to three insecticides, abamectin, dinotefuran, and β -cypermethrin. Seven genes were significantly up-regulated by abamectin exposure. Moreover, five and four genes were significantly up-regulated with dinotefuran and β -cypermethrin exposure, respectively, demonstrating their involvement in the detoxification of these such toxic substances in Z. cucurbitae. One example of these genes, ZcGSTe4 , was randomly selected to explore its function in response to β -cypermethrin exposure. Over-expressed ZcGSTe4 in E. coli showed significant tolerance to β -cypermethrin, and RNAi-mediated suppression of ZcGSTe4 also increased the sensitivity of melon fly to this agent. This study provides a foundation for further studies on the mechanism of detoxification metabolism in the melon fly. [ABSTRACT FROM AUTHOR]

Mechanistic investigations have shown that, upon agonist activation, hydroxy-carboxylic acid receptor-1(HCA1) couples to a Gi protein and inhibits adenylate cyclase activity, leading to inhibition of liberation of free fatty acid. However, the underlying molecular mechanisms for HCA1 signaling remain largely unknown. Using CHO-K1 cells stably expressing HCA1, and L6 cells, which endogenously express rat HCA1 receptors, we found that activation of ERK1/2 by HCA1 was rapid, peaking at 5 min, and was significantly blocked by pertussis toxin. Furthermore, time course experiments with different kinase inhibitors demonstrated that HCA1 induced ERK1/2 activation via the extracellular Ca2+, PKC and IGF-I receptor transactivation-dependent pathways. In addition, we observed that pretreated the cells with M119K, an inhibitor of Gβγ subunit-dependent signaling, effectively attenuated the ERK1/2 activation triggered by HCA1, suggesting a critical role for βγ-subunits in HCA1-activated ERK1/2 phosphorylation. Furthermore, the present results also indicated that the arrestin2/3 were not required for ERK1/2 activation. In conclusion, our findings demonstrate that upon binding to agonist, HCA1 receptors initially activate Gi, leading to dissociation of the Gβγ subunit from activated Gi, and subsequently induce ERK1/2 activation via two distinct pathways: one PKC-dependent pathway and the other IGF-IR transactivation-dependent pathway. Our results provide the first in-depth evidence that defines the molecular mechanism of HCA1-mediated ERK1/2 activation. [ABSTRACT FROM AUTHOR]

The first efficient intermolecular addition of nitroalkanes to activated enynes for asymmetric synthesis of 2,3-allenoates is described. It is a new addition to the limited available strategies for catalytic asymmetric synthesis of allenoates. Enabled by a new bifunctional catalyst, a range of trisubstituted allenoates can be obtained in excellent chemical and optical purity. These allenoate products with a pendant 2-nitroethyl α-substituent are useful chiral building blocks. [ABSTRACT FROM AUTHOR]

Mesenchymal stem cell‐derived conditioned medium (MSC‐CM) improves cardiac function after myocardial infarction; however, this cardioprotective effect is moderate and transient. Lipopolysaccharide (LPS) pretreatment partially improves MSC‐CM‐mediated cardioprotective effects owing to the presence of paracrine factors. However, the mechanism underlying these improved effects remains unknown. To study the effect of LPS‐pretreated MSC‐CM on hypoxia/reoxygenation (H/R)‐induced injury, MSCs were treated with or without LPS (400 ng/mL) for 48 h, and the supernatant was collected (MSC‐CM). Subsequently, H9c2 cells were co‐cultured with Nor‐CM (CM derived from LPS‐untreated MSCs) and LPS‐CM (CM derived from LPS‐pretreated MSCs) for 24 h and subjected to H/R. MSC‐CM inhibited the progression of H/R‐induced injury in H9c2 cells, and this protective effect was enhanced via LPS pretreatment as evidenced by the improved apoptosis assessment index (i.e. caspase‐3 and B‐cell lymphoma‐2 [Bcl‐2] expression) and decreased levels of lactic dehydrogenase (LDH) and cardiac troponin (cTn). In addition, the results of haematoxylin–eosin staining (H&E), transmission electron microscopy (TEM) and TdT‐mediated dUTP nick‐end labelling (TUNEL) validated that MSC‐CM inhibited H/R‐induced injury in H9c2 cardiomyocytes. LPS pretreatment downregulated the expression of high mobility group box‐1 (HMGB1) and BTB and CNC homology‐1 (Bach1) proteins in MSCs but upregulated the expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF) and insulin‐like growth factor (IGF). HMGB1 knockdown (MSC/siHMGB1‐CM) significantly decreased the expression of Bach1 and increased the expression of VEGF, HGF and IGF. Bach1 knockdown (MSC/siBach1‐CM) did not alter the production of HMGB1 but increased the expression of VEGF and IGF. LPS pretreatment did not alter the expression of the paracrine factors VEGF and HGF in the MSC/siHMGB1 group but increased their expression in the MSC/siBach1 group. The myocyte anti‐apoptotic effects of MSCs/siBach1‐CM were similar to those of untreated MSCs, which were not enhanced by LPS. LPS‐pretreated MSC‐CM protects H9c2 cells against H/R‐induced injury partly through the HMGB1/Bach1 signalling pathway. [ABSTRACT FROM AUTHOR]

Calsequestrin 1 (CASQ1) is the most crucial Ca2+ binding protein localized in the sarcoplasmic reticulum (SR) of skeletal muscle. With high capacity and low affinity for Ca2+, CASQ1 plays a significant role in maintaining a large amount of Ca2+ necessary for muscle contraction. However, only five mutations in CASQ1 have been identified to date. Here, we report a 42‐year‐old Chinese female patient who presented with a 12 years history of slowly progressive upper limb weakness, predominantly affecting distal muscles, which was uncommon comparing to other CASQ1‐related patients. Next‐generation sequencing (NGS) analysis revealed a novel heterozygous mutation (c.766G > A, p.Val256Met) in CASQ1. Functional studies confirmed the likely pathogenicity of this variant. Muscle histopathology revealed rare optically empty vacuoles in myofibers and atypical eosinophilic granules in the cytoplasm, which has not been observed before. We also performed a literature review on all the pathogenic mutations in CASQ1 and summarized their genetic and clinical characteristics. This is the first report on CASQ1‐related myopathy from China, further expanding the mutation spectrum of CASQ1 gene and provides new insights into the function of CASQ1. [ABSTRACT FROM AUTHOR]

• Five organotin complexes derived from hydroxycinnamic acids were synthesized. • These complexes exhibit 1D, 2D and 3D supramolecular structures. • The n -butyl and phenyl organotin complexes exhibit high cytostatic activity. • These complexes could quench the intrinsic fluorescence of BSA in a static way. Five new organotin(IV) hydroxycinnamates, [(Me 3 Sn)HL1] n (1), [(Ph 3 Sn) 2 L1] n (2), (n -Bu 2 Sn)(HL1) 2 (3), [(Me 3 Sn)HL2] n (4), [(Ph 3 Sn) 2 L2] n (5), have been synthesized by the reactions of R 3 SnCl (R = Me, Ph) or n -Bu 2 SnO with the corresponding hydroxycinnamic acid proligands [H 2 L1 = 4-OH-3-CH 3 OC 6 H 3 CH=CHCOOH, ferulic acid; H 2 L2 = 4-OH-3,5-(CH 3 O) 2 C 6 H 2 CH=CHCOOH, sinapic acid]. All the complexes have been characterized by X-ray diffraction analysis, elemental analysis, NMR (1H, 13C and 119Sn), FT-IR, and PXRD (Powder X-ray diffraction). The structural analyses reveal that complexes 1, 2 and 5 display 1D infinite zig-zag chain structure, in which the 1D chains are further linked into a 3D supramolecular structure through intermolecular O-H ··· O interactions (for complex 1) or C-H ··· O interactions (for complex 5). Complex 3 presents mononuclear tin monomer and further constructs 2D planar supramolecular structure through intermolecular O-H ··· O interactions. Complex 4 displays 1D right-handed helical chain and further connects into a 3D supramolecular architecture through intermolecular O-H ··· O interactions. Furthermore, the in vitro cytostatic activity of complexes 1-5 was preliminarily evaluated by MTT method, and the results showed that complexes 2, 3 and 5 exhibited high cytostatic activity. The protein-binding properties of complexes 2, 3 and 5 were also investigated, which indicated these complexes could quench the intrinsic fluorescence of BSA in a static way. Five new organotin(IV) complexes have been designed and synthesized from the reactions of two hydroxycinnamic acids (ferulic acid and sinapic acid) with the corresponding triorganotin(IV) chloride or diorganotin(IV) oxide. Meanwhile, complexes 1 - 5 were evaluated for their in vitro cytostatic activity against HeLa cell lines and HepG-2 cell lines. And partial complexes were evaluated for their binding interactions with BSA. [Display omitted] [ABSTRACT FROM AUTHOR]

This paper presents a preliminary study on the evaluation of an innovative energy dissipation system with shape memory alloys (SMAs) for structural seismic protection. A recentering shapememory alloy damper (RSMAD), in which superelastic nitinol wires are utilized as energy dissipation components, is proposed. Improved constitutive equations based on Graesser and Cozzarelli model are proposed for superelastic nitinol wires used in the damper. Cyclic tensile-compressive tests on the damper with various prestrain under different loading frequencies and displacement amplitudes were conducted. The results show that the hysteretic behaviors of the damper can bemodified to best fit the needs for passive structural control applications by adjusting the pretension of the nitinol wires, and the damper performance is not sensitive to frequencies greater than 0.5Hz. To assess the effectiveness of the dampers for structural seismic protection, nonlinear time history analysis on a ten-story steel frame with and without the dampers subjected to representative earthquake ground motions was performed. The simulation results indicate that superelastic SMA dampers are effective in mitigating the structural response of building structures subjected to strong earthquakes. [ABSTRACT FROM AUTHOR]

Shapememory alloys (SMAs) are a relatively new class of functional materials, exhibiting special thermo mechanical behaviors, such as shapememory effect and superelasticity, which enable their applications in seismic engineering as energy dissipation devices. This paper investigates the properties of superelastic NiTi shape memory alloys, emphasizing the influence of strain rate on superelastic behavior under various strain amplitudes by cyclic tensile tests. A novel constitutive equation based on Graesser and Cozzarelli's model is proposed to describe the strain-rate-dependent hysteretic behavior of superelastic SMAs at different strain levels. A stress variable including the influence of strain rate is introduced into Graesser and Cozzarelli's model. To verify the effectiveness of the proposed constitutive equation, experiments on superelastic NiTi wires with different strain rates and strain levels are conducted. Numerical simulation results based on the proposed constitutive equation and experimental results are in good agreement. The findings in this paper will assist the future design of superelastic SMA-based energy dissipation devices for seismic protection of structures. [ABSTRACT FROM AUTHOR]

Background: Mesenchymal stem cells (MSCs) promote tumor growth by differentiating into carcinoma-associated fibroblasts (CAFs) and composing the tumor microenvironment. However, the mechanisms responsible for the transition of MSCs to CAFs are not well understood. Exosomes regulate cellular activities by mediating cell-cell communication. In this study, we aimed to investigate whether cancer cell-derived exosomes were involved in regulating the differentiation of human umbilical cord-derived MSCs (hucMSCs) to CAFs. Methodology/Principal Findings: We first showed that gastric cancer cell-derived exosomes induced the expression of CAF markers in hucMSCs. We then demonstrated that gastric cancer cell-derived exosomes stimulated the phosphorylation of Smad-2 in hucMSCs. We further confirmed that TGF-β receptor 1 kinase inhibitor attenuated Smad-2 phosphorylation and CAF marker expression in hucMSCs after exposure to gastric cancer cell-derived exosomes. Conclusion/Significance: Our results suggest that gastric cancer cells triggered the differentiation of hucMSCs to CAFs by exosomes-mediated TGF-β transfer and TGF-β/Smad pathway activation, which may represent a novel mechanism for MSCs to CAFs transition in cancer. [ABSTRACT FROM AUTHOR]

Human umbilical cord–derived mesenchymal stem cells (hucMSCs) are particularly attractive cells for cellular and gene therapy in acute kidney injury (AKI). Adenovirus-mediated gene therapy has been limited by immune reaction and target genes selection. However, in the present study, we investigated the therapeutic effects of hepatocyte growth factor modified hucMSCs (HGF-hucMSCs) in ischemia/reperfusion-induced AKI rat models. In vivoanimal models were generated by subjecting to 60 min of bilateral renal injury by clamping the renal pedicles and then introduced HGF-hucMSCs via the left carotid artery. Our results revealed that serum creatinine and urea nitrogen levels decreased to the baseline more quickly in HGF-hucMSCs-treated group than that in hucMSCs- or green fluorescent protein-hucMSCs-treated groups at 72 h after injury. The percent of proliferating cell nuclear antigen-positive cells in HGF-hucMSCs-treated group was higher than that in the hucMSCs or green fluorescent protein-hucMSCs-treated groups. Moreover, injured renal tissues treated with HGF-hucMSCs also exhibited less hyperemia and renal tubule cast during the recovery process. Immunohistochemistry and living body imaging confirmed that HGF-hucMSCs localize to areas of renal injury. Real-time polymerase chain reaction result showed that HGF-hucMSCs also inhibited caspase-3 and interleukin-1β mRNA expression in injured renal tissues. Western blot also showed HGF-hucMSCs-treated groups had lower expression of interleukin-1β. Terminal deoxynucleotidyl transferase biotin-deoxyuridine triphosphate (dUTP) nick end labeling method indicated that HGF-hucMSCs-treated group had the least apoptosis cells. In conclusion, our findings suggest that HGF modification promotes the amelioration of ischemia/reperfusion-induced rat renal injury via antiapoptotic and antiinflammatory mechanisms; thus, providing a novel therapeutic application for hucMSCs in AKI. [ABSTRACT FROM AUTHOR]

Cancer stem cells (CSCs) have the characteristics of self-renewal, unlimited proliferation, and initiating new tumors. However, the origin of CSCs is still controversial. F6, a tumor cell line transformed from human fetal mesenchymal stem cells, was established in our previous study. Whether CSCs exist in this mutated cell line remains unclear. In the present study, we isolated CSCs from F6 cells based on CD133 expression using flow cytometry and investigated the biological characteristics of CD133+ F6 cells (F6-CD133+). We observed that the F6-CD133+ cells grew faster and had a higher capacity of colony formation than the F6-CD133− cells and parental F6 cells in vitro. In addition, F6-CD133+ cells had a higher tumorigenic ability than F6-CD133− cells in vivo since 1,000 F6-CD133+ cells were able to form tumors in nude mice. Cell viability assay revealed that F6-CD133+ cells were more sensitive to cisplatin while less to 5-fluorouracil. Furthermore, gene expression profile analysis showed that there were 673 differentially expressed genes between F6-CD133+ and F6-CD133− cells, many of which were involved in key cell signaling pathways. Taken together, our findings confirm that F6 cells contain a population of CSCs that contribute to its heterogeneity and tumorigenic potential, indicating that transformed stem cells could be the source of CSCs, and targeting this population may lead to more effective tumor treatments. [ABSTRACT FROM AUTHOR]

Cerebral malaria, a severe form of the disease, is one of the most severe complications of infection with Plasmodium parasites and a leading cause of malaria mortality. Currently available antimalarial therapy has proven insufficient to prevent neurological complications and death in all cases of cerebral malaria. Souza and colleagues observed that transplantation of bone marrow-derived mesenchymal stromal cells (BM-MSCs) increased survival, reduced parasitemia, decreased malaria pigment accumulation in the spleen, liver and kidney, elevated Kupffer cell count in liver, alleviated renal injury and lung inflammation, and improved lung mechanics in an experimental mouse model of cerebral malaria. Although plenty of challenges lie ahead, their findings show the promise of BM-MSC therapy for the treatment of cerebral malaria. [ABSTRACT FROM AUTHOR]

The feasibility of reinforcing conventional carbon fiber composites by grafting carbon nanotubes (CNTs) onto the fiber surface has been investigated. Carbon nanotubes were grown on carbon fibers using the chemical vapor deposition (CVD) method. Iron was selected as the catalyst and predeposited using the incipient wetness technique before the growth reaction. The morphology of the products was characterized using scanning electron microscopy (SEM), which showed evidence of a uniform coating of CNTs on the fiber surface. Contact angle measurements on individual fibers, before and after the CNT growth, demonstrated a change in wettability that can be linked to a change of the polarity of the modified surface. Model composites based on CNT-grafted carbon fibers/epoxy were fabricated in order to examine apparent interfacial shear strength (IFSS). A dramatic improvement in IFSS over carbon fiber/epoxy composites was observed in the single fiber pull-out tests, but no significant change was shown in the push-out tests. The different IFSS results were provisionally attributed to a change of failure mechanism between the two types of tests, supported by fractographic analysis. [ABSTRACT FROM AUTHOR]

We present a new method for the imaging of single metallic nanoparticles that provides information about their shape and orientation. Using confocal microscopy in combination with higher order laser modes, scattering images of individual particles are recorded. Gold nanospheres and nonorods render characteristic patterns reflecting the different particle geometries. In the case of nanorods, the scattering patterns also reveal the orientation of the particles. This novel technique provides a promising tool for the visualization of nonbleaching labels in the biosciences. [ABSTRACT FROM AUTHOR]

Studies the controllability and observability issues for a linear time-varying impulsive control system. Variation of parameter for impulsive control systems; Modeling of complex dynamical behaviors of the system.

Cerebral malaria, a severe form of the disease, is one of the most severe complications of infection with Plasmodium parasites and a leading cause of malaria mortality. Currently available antimalarial therapy has proven insufficient to prevent neurological complications and death in all cases of cerebral malaria. Souza and colleagues observed that transplantation of bone marrow-derived mesenchymal stromal cells (BM-MSCs) increased survival, reduced parasitemia, decreased malaria pigment accumulation in the spleen, liver and kidney, elevated Kupffer cell count in liver, alleviated renal injury and lung inflammation, and improved lung mechanics in an experimental mouse model of cerebral malaria. Although plenty of challenges lie ahead, their findings show the promise of BM-MSC therapy for the treatment of cerebral malaria. [ABSTRACT FROM AUTHOR]

There have been numerous research efforts directed towards the synthesis of gold (Au) nanoparticles (NPs) and the understanding of their formation, so that their size, shape, and stability can be well controlled for desired applications. Here, we report a dry photo-reduced method of Au NP formation directly on a membrane, such as a carbon thin film or a quartz slide. The evolution of Au NP formation was revealed by ex-situ experiments in an aberration-corrected scanning transmission electron microscope. The membranes were immersed in Au3+ solution before being taken out and quickly dried in ambient air at room temperature, then irradiated with ultraviolet (UV) light with wavelengths of 189 nm and 254 nm in a low-pressure chamber. The results show that Au3+ ions and ion clusters self-assembled on the membrane surface before UV irradiation and that solid Au NPs with sizes of 3 nm-12 nm were formed after UV irradiation. Annealing at 40 °C for about 30 min helped to further stabilize the nanoparticles. The Au NPs were uniform and well dispersed, and should find applications in the electron microscopy field, for example. [ABSTRACT FROM AUTHOR]

Simple Summary: The melon fly, Zeugodacus cucurbitae, is an important agricultural pest. At present, chemical pesticide treatment is the main method for field control, but this promotes pesticide resistance by Z. cucurbitae, because of its frequent use. ABC transporters are involved in detoxification metabolism, but few studies have yet considered their expression in melon fly. In this study, we identified the ABC transporters genes at a genome-wide level in melon fly, and analysed their spatiotemporal expression patterns, as well as changes in expression after insecticides treatments. A total of 49 ABC transporters were identified, and their expression levels varied at different developmental stages and between tissues. After three insecticides treatment, ZcABCB7 and ZcABCC2 were up-regulated. After β-cypermethrin induction, tissues were dissected at 12, 24 and 48 h, and the expression levels of a number of ABC genes were highly expressed within the fat body. From these results, we conclude that ZcABCB7 and ZcABCC2 may be involved in detoxification metabolism, and that the fat body is the main tissue that plays this role. The ATP-binding cassette (ABC) transporter is a protein superfamily that transports specific substrate molecules across lipid membranes in all living species. In insects, ABC transporter is one of the major transmembrane protein families involved in the development of xenobiotic resistance. Here, we report 49 ABC transporter genes divided into eight subfamilies (ABCA-ABCH), including seven ABCAs, seven ABCBs, 10 ABCCs, two ABCDs, one ABCE, three ABCFs, 16 ABCGs, and three ABCHs according to phylogenetic analysis in Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. The expressions level of 49 ABC transporters throughout various developmental stages and within different tissues were evaluated by quantitative transcriptomic analysis, and their expressions in response to three different insecticides were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). These ABC transporter genes were widely expressed at developmental stages but most highly expressed in tissues of the midgut, fat body and Malpighian tube. When challenged by exposure to three insecticides, abamectin, β-cypermethrin, and dinotefuran, the expressions of ZcABCB7 and ZcABCC2 were significantly up-regulated. ZcABCB1, ZcABCB6, ZcABCB7, ZcABCC2, ZcABCC3, ZcABCC4, ZcABCC5, and ZcABCC7 were significantly up-regulated in the fat body at 24 h after β-cypermethrin exposure. These data suggest that ZcABCB7 and ZcABCC2 might play key roles in xenobiotic metabolism in Z. cucurbitae. Collectively, these data provide a foundation for further analysis of ABCs in Z. cucurbitae. [ABSTRACT FROM AUTHOR]

• Synergistic interaction between sodium cholate and Tween 80 were explored. • The effect of the crowding media on the digestion varies with oil compositions. • The lipolysis rate and transformation increased when using binary emulsifiers. • The denaturation temperature of lipase changed in the crowding media. • The lipolysis in crowding media was affected by the stability of lipase. Control of lipid digestibility by various food components has received great attention in recent decades. However, there is limited literature on investigating the synergistic effect of exogenous emulsifiers and endogenous sodium cholate (SC) on lipid digestion in a simulated physiological crowded medium. In this work, the synergistic interaction of Tween80 and SC according to the regular solution theory, and the hydrolysis of lipid emulsions containing tricaprylin, glyceryltrioleate or soybean oil in crowding medium was studied. The results show that emulsions stabilized by a combination of Tween80 and SC showed higher digestion rate and transformation than those with Tween80 or SC. The digestion rate could be increased by polyethylene glycols (PEGn) with varying crowding degree. The denaturation temperature of the lipase was increased in macromolecular crowded medium. This work allows for better understanding of the interaction between the amphiphiles and the macromolecular crowding effect on lipase digestion in the physiological environment. [ABSTRACT FROM AUTHOR]

Vitelline membrane proteins (VMPs) are major components of the vitelline membrane, which is an essential component of insect eggshell. The melon fly, Zeugodacus cucurbitae (Coquillett), is a destructive pest of many fruits and vegetables. Here, we identified and characterized three VMP genes in Z. cucurbitae, including ZcVMP26Aa, ZcVMP26Aalike, and ZcVMP26Ab. The open reading frames of ZcVMP26Aa, ZcVMP26Aa-like, and ZcVMP26Ab contained 384, 348, and 438 bp, encoding 127, 115, and 145 amino acid residues, respectively. A signal peptide was predicted in the 5'-end of each ZcVMPs. A conserved domain was also predicted within the three ZcVMPs. A phylogenetic tree of insect VMPs showed that the ZcVMPs are closely related to VMPs in Drosophila melanogaster. Spatiotemporal expression analyses showed that all three genes were highly expressed in adult females especially during the vitellogenesis period. Among the different tissues examined, the three genes only had high expression in the ovary. Suppression of the ZcVMP26Ab transcription by RNA interference significantly decreased the target gene expression and resulted in the increased desiccation and low hatchability of newly laid eggs. This indicated an important role of ZcVMP26Ab in preventing water loss in Z. cucurbitae embryos. [ABSTRACT FROM AUTHOR]

The glutathione (GSH) system is one of the most powerful intracellular antioxidant systems for the elimination of reactive oxygen species (ROS) and maintaining cellular redox homeostasis. However, the rapid kinetics information (at the millisecond to the second level) during the dynamic antioxidation process of the GSH system remains unclear. As such, we specifically developed a novel dual‐wire nanosensor (DWNS) that can selectively and synchronously measure the levels of GSH and ROS with high temporal resolution, and applied it to monitor the transient ROS generation as well as the rapid antioxidation process of the GSH system in individual cancer cells. These measurements revealed that the glutathione peroxidase (GPx) in the GSH system is rapidly initiated against ROS burst in a sub‐second time scale, but the elimination process is short‐lived, ending after a few seconds, while some ROS are still present in the cells. This study is expected to open new perspectives for understanding the GSH antioxidant system and studying some redox imbalance‐related physiological. [ABSTRACT FROM AUTHOR]

The glutathione (GSH) system is one of the most powerful intracellular antioxidant systems for the elimination of reactive oxygen species (ROS) and maintaining cellular redox homeostasis. However, the rapid kinetics information (at the millisecond to the second level) during the dynamic antioxidation process of the GSH system remains unclear. As such, we specifically developed a novel dual‐wire nanosensor (DWNS) that can selectively and synchronously measure the levels of GSH and ROS with high temporal resolution, and applied it to monitor the transient ROS generation as well as the rapid antioxidation process of the GSH system in individual cancer cells. These measurements revealed that the glutathione peroxidase (GPx) in the GSH system is rapidly initiated against ROS burst in a sub‐second time scale, but the elimination process is short‐lived, ending after a few seconds, while some ROS are still present in the cells. This study is expected to open new perspectives for understanding the GSH antioxidant system and studying some redox imbalance‐related physiological. [ABSTRACT FROM AUTHOR]

Extracellular vesicles (EVs) in the field of spinal cord injury (SCI) have garnered significant attention for their potential applications in diagnosis and therapy. However, no bibliometric assessment has been conducted to evaluate the scientific progress in this area. A search of articles in Web of Science (WoS) from January 1, 1991, to May 1, 2023, yielded 359 papers that were analyzed using various online analysis tools. These articles have been cited 10,842 times with 30.2 times per paper. The number of publications experienced explosive growth starting in 2015. China and the United States led this research initiative. Keywords were divided into 3 clusters, including "Pathophysiology of SCI", "Bioactive components of EVs", and "Therapeutic effects of EVs in SCI". By integrating the average appearing year (AAY) of keywords in VoSviewer with the time zone map of the Citation Explosion in CiteSpace, the focal point of research has undergone a transformative shift. The emphasis has moved away from pathophysiological factors such as "axon", "vesicle", and "glial cell" to more mechanistic and applied domains such as "activation", "pathways", "hydrogels" and "therapy". In conclusions, institutions are expected to allocate more resources towards EVs-loaded hydrogel therapy and the utilization of innovative materials for injury mitigation. [ABSTRACT FROM AUTHOR]

The application of an in situ potential difference between carbon fibres and a graphite foil counter electrode (300 V, generating an electric field ca 0.3–0.7 V μm−1), during the chemical vapour deposition synthesis of carbon nanotube (CNT) grafted carbon fibres, significantly improves the uniformity of growth without reducing the tensile properties of the underlying carbon fibres. Grafted CNTs with diameters 55 nm ± 36 nm and lengths around 10 μm were well attached to the carbon fibre surface, and were grown without the requirement for protective barrier coatings. The grafted CNTs increased the surface area to 185 m2 g−1 compared to the as-received sized carbon fibre 0.24 m2 g−1. The approach is not restricted to batch systems and has the potential to improve CNT grafted carbon fibre production for continuous processing. [ABSTRACT FROM AUTHOR]

The article offers information on a study based on catalytic asymmetric synthesis of the enantio-enriched racemic 2, 3-allenoates. Topics discussed include a new cinchona-based bifunctional catalyst which promotes the addition of nitro-alkanes, isomerization of alkynoate products to form final allenoate products, and synthesis of enantioenriched 2, 3-allenoates using nitroalkane nucleophiles. It also presents brief profile of authors involved in this study.

How to efficiently and physically integrate the soil water dynamics with groundwater flow processes has drawn much attention. We present a coupled soil water-groundwater model, considering the two-way feedback coupling scheme, and verified its performance using two synthetic cases (using the fully 3D variably saturated flow (VSF) model simulations as the 'reference') and one real catchment 25 case (using the groundwater table depth and soil moisture profile measurements). By the cross-validation between the observations and various model simulations, the two-way coupling approach is proven physically accurate and is applicable for large-scale groundwater flow problems. Compared to the simulation by groundwater model alone (i.e., only MODFLOW), the coupling of MODFLOW with one soil column reduced the overestimation of groundwater table simulation (taking the VSF model simulations as the reference). The results were further improved as more soil columns were used to represent the heterogeneous soil watergroundwater interactions. Compared to the HYDRUS-MODFLOW, the two-way coupling approach produces a similar spatial distribution of hydraulic heads while better performs in mimicking the temporal dynamics of groundwater table depth and soil moisture profiles. We attribute the better performance to the different coupling strategies across the soil-water and groundwater interface. It is thus suggested to adopt the two-way feedback coupling scheme, together with the moving phreatic boundary and multi-scale water balance analysis, to maintain physical consistency and reduce coupling errors. The realistic implementation of the vadose zone processes (with STEMMUS), coupling approach, and spatiotemporal heterogeneity of soil water-groundwater interactions were demonstrated critical to accurately represent an integrated soil water-groundwater system. The developed STEMMUS-MODFLOW model can be further equipped with different complexities of soil physics (e.g., coupled soil water and heat transfer, freeze-thaw, airflow processes), surface hydrology (snowfall, runoff), soil and plant biogeochemical processes, towards an integrated "from bedrock to atmosphere" modeling framework. [ABSTRACT FROM AUTHOR]

Background: Artificial intelligence (AI) technology is a promising diagnostic adjunct in fracture detection. However, few studies describe the improvement of clinicians' diagnostic accuracy for nasal bone fractures with the aid of AI technology.Objective: This study aims to determine the value of the AI model in improving the diagnostic accuracy for nasal bone fractures compared with manual reading.Methods: A total of 252 consecutive patients who had undergone facial computed tomography (CT) between January 2020 and January 2021 were enrolled in this study. The presence or absence of a nasal bone fracture was determined by two experienced radiologists. An AI algorithm based on the deep-learning algorithm was engineered, trained and validated to detect fractures on CT images. Twenty readers with various experience were invited to read CT images with or without AI. The accuracy, sensitivity and specificity with the aid of the AI model were calculated by the readers.Results: The deep-learning AI model had 84.78% sensitivity, 86.67% specificity, 0.857 area under the curve (AUC) and a 0.714 Youden index in identifying nasal bone fractures. For all readers, regardless of experience, AI-aided reading had higher sensitivity ([94.00 ± 3.17]% vs [83.52 ± 10.16]%, P< 0.001), specificity ([89.75 ± 6.15]% vs [77.55 ± 11.38]%, P< 0.001) and AUC (0.92 ± 0.04 vs 0.81 ± 0.10, P< 0.001) compared with reading without AI. With the aid of AI, the sensitivity, specificity and AUC were significantly improved in readers with 1-5 years or 6-10 years of experience (all P< 0.05, Table 4). For readers with 11-15 years of experience, no evidence suggested that AI could improve sensitivity and AUC (P= 0.124 and 0.152, respectively).Conclusion: The AI model might aid less experienced physicians and radiologists in improving their diagnostic performance for the localisation of nasal bone fractures on CT images. [ABSTRACT FROM AUTHOR]

In the present study, the quality of groundwater for drinking purposes was evaluated. The following parameters were analyzed using American Public Health Association standard method: pH, total hardness (TH), total dissolved solids (TDS), electrical conductivity (EC), bicarbonate (HCO3-), chloride (Cl-), sulphate (SO4 2-), fluoride (F-), calcium (Ca2+), magnesium (Mg2+), sodium (Na+) and potassium (K+). These values were compared with limits recommended by the World Health Organization (WHO) and the Bureau of Indian Standards (BIS) for drinking purposes. The cation and anion dominance of the study region groundwater samples were Na+ > Ca2+ > Mg2+ > K+ and HCO3- > Cl- > SO4 2- > F-, respectively. Bicarbonate was the dominant anion and Na+ was identified as the dominant cation in the groundwater of the study region. The Na+ and Cl- concentration of 43% and 37% of groundwater samples were found to be more than the acceptable limit of WHO in the study region. Most of the groundwater samples in the study region were categorized as a very hard category. The groundwater was highly affected by the fluoride and about 60% of groundwater samples were unfit for drinking purposes in the study region. [ABSTRACT FROM AUTHOR]

Contamination of soil potentially toxic elements (PTEs) has observed in many parts of the world, specifically developing countries such as India. In this study, we only obtained the available data in the literature from 2001 to 2020 on soil PTEs in India. To evaluate the soil contamination, geo-accumulation index and contamination factor (CF) were implemented, along with the health risk assessment was also applied which was proposed by the US Environmental Protection Agency. The mean values obtained for soils of India are Cr (412.28mg/kg), Cu (159.78mg/kg), Zn (437.44), Pb (77.25mg/kg) and As (38.61 mg/kg). The geo-accumulation index and CF showed that Cu, Zn, Ni and As are the main contaminants. The results of health risk show that children are susceptible to both non-carcinogenic and carcinogenic risks of the studied PTEs compared to adults. [ABSTRACT FROM AUTHOR]

Complement activation and prefrontal cortical dysfunction both contribute to the pathogenesis of major depressive disorder (MDD), but their interplay in MDD is unclear. We here studied the role of complement C3a receptor (C3aR) in the medial prefrontal cortex (mPFC) and its influence on depressive-like behaviors induced by systematic lipopolysaccharides (LPS) administration. C3aR knockout (KO) or intra-mPFC C3aR antagonism confers resilience, whereas C3aR expression in mPFC neurons makes KO mice susceptible to LPS-induced depressive-like behaviors. Importantly, the excitation and inhibition of mPFC neurons have opposing effects on depressive-like behaviors, aligning with increased and decreased excitability by C3aR deletion and activation in cortical neurons. In particular, inhibiting mPFC glutamatergic (mPFCGlu) neurons, the main neuronal subpopulation expresses C3aR, induces depressive-like behaviors in saline-treated WT and KO mice, but not in LPS-treated KO mice. Compared to hypoexcitable mPFCGlu neurons in LPS-treated WT mice, C3aR-null mPFCGlu neurons display hyperexcitability upon LPS treatment, and enhanced excitation of mPFCGlu neurons is anti-depressant, suggesting a protective role of C3aR deficiency in these circumstances. In conclusion, C3aR modulates susceptibility to LPS-induced depressive-like behaviors through mPFCGlu neuronal excitability. This study identifies C3aR as a pivotal intersection of complement activation, mPFC dysfunction, and depression and a promising therapeutic target for MDD. • C3aR expression in mPFC neurons renders susceptibility to LPS-induced depressive-like behaviors. • C3aR inversely regulates excitability in primary cortical and mPFCGlu neurons. • LPS causes reduced mPFCGlu neuronal excitability, thereby triggering depressive-like behaviors in WT mice. • C3aR deletion reverses the direction of LPS-induced excitability changes in mPFCGlu neurons. [ABSTRACT FROM AUTHOR]

The eukaryotic calcium/calmodulin-dependent protein phosphatase calcineurin is crucial for the environmental adaption of fungi. However, the mechanism of coordinate regulation of the response to salt stress by calcineurin and the high-affinity calcium channel CchA in fungi is not well understood. Here we show that the deletion of cchA suppresses the hyphal growth defects caused by the loss of calcineurin under salt stress in Aspergillus nidulans. Additionally, the hypersensitivity of the ΔcnaA strain to extracellular calcium and cell-wall-damaging agents can be suppressed by cchA deletion. Using the calcium-sensitive photoprotein aequorin to monitor the cytoplasmic Ca2+ concentration ([Ca2+]c) in living cells, we found that calcineurin negatively regulates CchA on calcium uptake in response to external calcium in normally cultured cells. However, in salt-stress-pretreated cells, loss of either cnaA or cchA significantly decreased the [Ca2+]c, but a deficiency in both cnaA and cchA switches the [Ca2+]c to the reference strain level, indicating that calcineurin and CchA synergistically coordinate calcium influx under salt stress. Moreover, real-time PCR results showed that the dysfunction of cchA in the ΔcnaA strain dramatically restored the expression of enaA (a major determinant for sodium detoxification), which was abolished in the ΔcnaA strain under salt stress. These results suggest that double deficiencies of cnaA and cchA could bypass the requirement of calcineurin to induce enaA expression under salt stress. Finally, YvcA, a member of the transient receptor potential channel (TRPC) protein family of vacuolar Ca2+ channels, was proven to compensate for calcineurin-CchA in fungal salt stress adaption. [ABSTRACT FROM AUTHOR]

Mesenchymal stem cell-derived conditioned medium (MSC-CM) improves cardiac function, which is partly attributed to the released paracrine factors. Since such cardioprotection is moderate and transient, it is essential that MSC-CM's effective components are optimized to alleviate myocardial injury. To optimize MSC-CM, MSCs were treated with or without lipopolysaccharides (LPSs) for 48 h (serum-free), and the supernatant was collected. Then, LPS-CM (MSC stimulated by LPS) was further treated with LPS remover (LPS Re-CM) or was concentrated with a 10 kDa cutoff filter (10 kDa-CM). Enzyme-linked immunosorbent assay showed that all the pretreatments increased the levels of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin growth factor (IGF) except LPS Re-CM; 10 kDa-CM was superior to the other CMs. Cell Counting Kit-8 displayed that the viability of injured H9c2 cells was enhanced with the increase in the MSC-CM concentration. We also found that the 10 kDa-CM significantly alleviated H9c2 hypoxia/reoxygenation (H/R) injury, as evidenced by the increased Bcl-2/Bax ratio, and decreased the levels of lactate dehydrogenase and cardiac troponin. Transmission electron microscopy (TEM), TdT-mediated dUTP nick-end labelling (TUNEL), and hematoxylin and eosin staining (H&E) confirmed that 10 kDa-CM inhibited H/R-induced H9c2 morphological changes. Proteomic analysis identified 41 differentially expressed proteins in 10 kDa-CM, among which anti-inflammation, proangiogenesis, and antiapoptosis were related to cardiac protection. This study indicates that 10 kDa-CM protects H9c2 cardiomyocytes from H/R injury by preserving most of the protective factors, such as VEGF, HGF, and IGF, in MSC-CM. [ABSTRACT FROM AUTHOR]

Statistical analyses, a Piper diagram, the saturation index and the correlations of chemical parameters were used to reveal the hydrogeochemistry and hydrogeochemical evolution of shallow groundwater in the southern part of the Zhongwei section of the Yellow River alluvial plain. The water quality for agricultural and domestic uses was also assessed in the study. The results suggest that the shallow groundwater in the study area is fresh to moderately mineralized water. Higher Ca2+ and HCO3- are observed in the less mineralized water, whereas Na+ and SO42- are common ions in the highly mineralized water. The major hydrochemical facies for groundwater with total dissolved solids (TDS) <1 g/L are HCO3-Ca·Mg and HCO3-Ca·Na·Mg, and for groundwater with TDS > 1 g/L, SO4·Cl-Na and SO4·Cl-Na·Mg·Ca are the predominant hydrochemical types. The main reactions in the groundwater system are the dissolution/precipitation of gypsum, fluorite, halite, calcite and dolomite. Cation exchange is also important in controlling the groundwater chemistry. The water samples assessed in the paper are of acceptable quality for agricultural use, but most of them are not fit for direct human consumption (drinking). TDS, total hardness (TH), Cl- and SO42- are the main indices that result in the poor drinking water quality. [ABSTRACT FROM AUTHOR]