29 results on '"Heissigerova J"'
Search Results
2. Metronidazole Attenuates the Intensity of Inflammation in Experimental Autoimmune Uveitis
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Seidler Stangova, P., primary, Dusek, O., additional, Klimova, A., additional, Heissigerova, J., additional, Kucera, T., additional, and Svozilkova, Petra, additional
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- 2019
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3. Metronidazole Attenuates the Intensity of Inflammation in Experimental Autoimmune Uveitis.
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STANGOVA, P. SEIDLER, DUSEK, O., KLIMOVA, A., HEISSIGEROVA, J., KUCERA, T., and SVOZILKOVA, P.
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UVEITIS ,METRONIDAZOLE ,CIPROFLOXACIN ,PERTUSSIS toxin ,BIOTHERAPY ,CARRIER proteins - Abstract
Autoimmune uveitis is a serious sightthreatening disease that in many cases fails to respond to conventional immunosuppressive or biological therapy. Experimental models used in research allow more detailed study of pathogenesis of the autoimmune process and testing new therapeutic strategies. Recent results show that infection can trigger autoimmune diseases, and some commensal microorganisms are essential in causing disease activity. The aim of this work was to assess the effect of broadspectrum antibiotics - combination of metronidazole and ciprofloxacin or metronidazole alone - on the intensity of intraocular inflammation in experimental autoimmune uveitis (EAU). EAU was induced in mouse strain C57BL/6J by interphotoreceptor retinoid- binding protein in complete Freund's adjuvant and pertussis toxin. The grade of uveitis was assessed clinically and histologically in haematoxylin and eosin- stained tissues. Lymphocytes and macrophages were detected in cryosections using the immunoperoxidase method with antibodies. The therapy was commenced one week before EAU induction and continued throughout the experiment. In addition, metronidazole treatment was also started two weeks before EAU induction. Antibiotics significantly reduced the intensity of uveitis compared to the control group (P < 0.05). The effects of combination of ciprofloxacin and metronidazole and of metronidazole alone were similar when the therapy started one week before EAU induction (P < 0.05). Metronidazole commenced two weeks before EAU induction and throughout the experiment suppressed the intensity of EAU with even higher statistical significance (P < 0.0001). It can be assumed that the high protective effect of metronidazole on EAU intensity may be due not only to its antimicrobial effect, but also to its immunomodulatory activity. [ABSTRACT FROM AUTHOR]
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- 2019
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4. Microbiota diversity in patients with Diffuse Large B‐Cell Lymphoma and Immune‐Privileged Sites Lymphoma.
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Dlouha, L., Tenglerova, M., Rehakova, J., Brichova, M., Kostovcik, M., Benesova, K., Kverka, M., Heissigerova, J., Svozilkova, P., Ondeckova, I., Senavova, J., Herman, V., Kostovcikova, K., and Trneny, M.
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DIFFUSE large B-cell lymphomas ,LYMPHOMAS - Abstract
Microbiota diversity in patients with Diffuse Large B-Cell Lymphoma and Immune-Privileged Sites Lymphoma There is however still limited knowledge about MB diversity in different lymphoma subtypes especially in large B-cell lymphoma of immune-privileged sites (LBCL-IPS). B Conclusions: b Both lymphoma groups - diffuse large B-cell lymphoma as well as large B-cell lymphoma of immune-privileged sites - are characterized by unique microbiota composition. [Extracted from the article]
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- 2023
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5. Sarilumab for the treatment of posterior segment non-infectious uveitis (NIU): The SATURN (SARIL-NIU) study
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Heissigerova, J., primary, Sundaram, P.A., additional, Erickson, K., additional, Varona, R., additional, Corp-dit-Genti, V., additional, Vitti, R., additional, Buggage, R., additional, Rihova, E., additional, and Nguyen, Q.D., additional
- Published
- 2015
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6. Mycophenolate mofetil and cyclophosphamide treatment suppress inflammation intensity in experimental model of autoimmune uveitis
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KLIMOVA, A, primary, SEIDLER STANGOVA, P, additional, HEISSIGEROVA, J, additional, SVOZILKOVA, P, additional, and KUCERA, T, additional
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- 2014
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7. Tumor necrosis factor polymorphisms associated with tumor necrosis factor production influence the risk of idiopathic intermediate uveitis
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Atan, D., Heissigerova, J., Kuffová, L., Hogan, A., Kilmartin, D. J., Forrester, J. V., Bidwell, J. L., Andrew Dick, and Churchill, A. J.
8. A possible coincidence of cytomegalovirus retinitis and intraocular lymphoma in a patient with systemic non-Hodgkin’s lymphoma
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Svozílková Petra, Heissigerová Jarmila, Brichová Michaela, Kalvodová Bohdana, Dvořák Jan, and Říhová Eva
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Cytomegalovirus ,Cytomegalovirus retinitis ,Foscarnet ,Non-Hodgkin’s lymphoma ,Rituximab ,Valganciclovir ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Purpose To present a possible coincidence of cytomegalovirus retinitis and intraocular lymphoma in a patient with systemic non-Hodgkin’s lymphoma. Case presentation A 47-year-old woman presented with decreased visual acuity associated with white retinal lesions in both eyes. A history of pneumonia of unknown aetiology closely preceded the deterioration of vision. Five years previously the patient was diagnosed with follicular non-Hodgkin’s lymphoma. She was treated with a chemotherapy regimen comprised of cyclophosphamide, adriamycin, vincristin, and prednisone with later addition of the anti-CD20 antibody rituximab. She experienced a relapse 19 months later with involvement of the retroperitoneal lymph nodes, and commenced treatment with rituximab and 90Y-ibritumomab tiuxetan. A second relapse occurred 22 months after radioimmunotherapy and was treated with a combination of fludarabine, cyclophosphamide, and mitoxantrone followed by rituximab. The patient experienced no further relapses until the current presentation (April, 2010). Pars plana vitrectomy with vitreous fluid analysis was performed in the right eye. PCR testing confirmed the presence of cytomegalovirus in the vitreous. Atypical lymphoid elements, highly suspicious of malignancy were also found on cytologic examination. Intravenous foscarnet was administered continually for three weeks, followed by oral valganciclovir given in a dose of 900 mg twice per day. In addition, the rituximab therapy continued at three monthly intervals. Nevertheless, cessation of foscarnet therapy was followed by a recurrence of retinitis on three separate occasions during a 3-month period instigating its reinduction to the treatment regime after each recurrence. Conclusions Cytomegalovirus retinitis is an opportunistic infection found in AIDS patients as well as in bone marrow and solid organ transplant recipients being treated with systemic immunosuppressive drugs. This case presents a less common incidence of cytomegalovirus retinitis occurring in a patient with non-Hodgkin’s lymphoma. We demonstrated a possible coexistence of cytomegalovirus retinitis and intraocular lymphoma in this particular patient. The final diagnosis was based on clinical manifestations together with the course of uveitis and its response to treatment alongside the results of vitreous fluid analysis. This report highlights the importance of intraocular fluid examination in cases with nonspecific clinical manifestations. Such an examination allows for the detection of simultaneously ongoing ocular diseases of differing aetiologies and enables the prompt initiation of effective treatment.
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- 2013
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9. Blau Syndrome: Challenging Molecular Genetic Diagnostics of Autoinflammatory Disease.
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Brichova M, Klimova A, Heissigerova J, Svozilkova P, Vaneckova M, Dolezalova P, Nemcova D, Michalickova M, Jedlickova J, Dudakova L, and Liskova P
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- Humans, Arthropathy, Neurogenic genetics, Arthropathy, Neurogenic diagnosis, Central Nervous System Diseases, Exome Sequencing, Hereditary Autoinflammatory Diseases, Lymphedema genetics, Lymphedema diagnosis, Arthritis genetics, Arthritis diagnosis, Mutation, Nod2 Signaling Adaptor Protein genetics, Pedigree, Sarcoidosis genetics, Sarcoidosis diagnosis, Synovitis genetics, Synovitis diagnosis, Uveitis genetics, Uveitis diagnosis
- Abstract
The aim of this study was to describe the clinical and molecular genetic findings in seven individuals from three unrelated families with Blau syndrome. A complex ophthalmic and general health examination including diagnostic imaging was performed. The NOD2 mutational hot spot located in exon 4 was Sanger sequenced in all three probands. Two individuals also underwent autoinflammatory disorder gene panel screening, and in one subject, exome sequencing was performed. Blau syndrome presenting as uveitis, skin rush or arthritis was diagnosed in four cases from three families. In two individuals from one family, only camptodactyly was noted, while another member had camptodactyly in combination with non-active uveitis and angioid streaks. One proband developed two attacks of meningoencephalitis attributed to presumed neurosarcoidosis, which is a rare finding in Blau syndrome. The probands from families 1 and 2 carried pathogenic variants in NOD2 (NM_022162.3): c.1001G>A p.(Arg334Gln) and c.1000C>T p.(Arg334Trp), respectively. In family 3, two variants of unknown significance in a heterozygous state were found: c.1412G>T p.(Arg471Leu) in NOD2 and c.928C>T p.(Arg310*) in NLRC4 (NM_001199139.1). In conclusion, Blau syndrome is a phenotypically highly variable, and there is a need to raise awareness about all clinical manifestations, including neurosarcoidosis. Variants of unknown significance pose a significant challenge regarding their contribution to etiopathogenesis of autoinflammatory diseases.
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- 2024
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10. Experimental model of primary intraocular lymphoma based on BALB/CaNn strain and A20 cells is optimal for investigational research.
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Skrlova E, Uherkova E, Klimova A, Malarikova D, Svozilkova P, Matous P, Herynek V, Kucera T, Klener P, and Heissigerova J
- Abstract
Aim: The purpose of this project was to compare the characteristics of two experimental murine models of primary intraocular lymphoma (PIOL) and determine which experimental model is most suitable for further investigational research to elucidate the pathophysiology of PIOL and to find new therapeutical strategies., Methods: In both experimental models PIOL was induced in immunocompetent mice with intravitreal injection of syngeneic B-cell lymphoma cell lines. Murine strain C3H/HeN and cell line 38C13 were used in the first model and BALB/CaNn mice and cell line A20 in the second model. During the experiments, thorough clinical evaluation (using photo documentation, ultrasonography, and MRI) and histological evaluation were performed., Results: In both models, the percentage of PIOL development was high, reaching nearly 80%. Disease progression was faster in C3H/HeN with exophthalmos occurring on average on day 10. Vitreous involvement was a predominant sign in the clinical presentation of this group. In BALB/CaNn mice exophthalmos occurred on average on day 22. The predominant clinical sign in the BALB/CaNn group was tumorous infiltration of the retina, optic disc, and tumorous retinal detachment., Conclusion: Slower progression of the disease in BALB/CaNn mice, greater possibility to examine the retina due to mild vitreous involvement, and later occurrence of exophthalmos makes this strain more suitable for further investigational research., Competing Interests: The authors report no conflicts of interest in this work.
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- 2024
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11. Ocular sarcoidosis in adults and children: update on clinical manifestation and diagnosis.
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Bazewicz M, Heissigerova J, Pavesio C, Willermain F, and Skrzypecki J
- Abstract
Sarcoidosis-associated uveitis, is the predominant ocular sarcoidosis presentation, which affects both adults and children. For adults, international ocular sarcoidosis criteria (IWOS) and sarcoidosis-associated uveitis criteria (SUN) are defined. However, for children they are not yet established internationally. Due to the specificity of pediatric manifestations of sarcoidosis, this task is even more challenging. In children, sarcoidosis is subdivided into Blau syndrome and early-onset sarcoidosis (BS/EOS) affecting younger children (< 5 years) and the one affecting older children with clinical presentation resembling adults. Differential diagnosis, clinical work-up as well as diagnostic criteria should be adapted to each age group. In this article, we review the clinical manifestation of sarcoidosis-associated uveitis in adults and children and the sensitivity and specificity of various ocular sarcoidosis diagnostic modalities, including chest X-ray and CT, FDG PET-CT, gallium-67 scintigraphy, bronchoalveolar lavage fluid, genetic testing for NOD2 mutations and serum biomarkers, such as ACE, lysozyme and IL2R., (© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)
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- 2023
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12. Trends in management of ocular syphilis in tertiary uveitis centre in Prague, Czech Republic.
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Klimova A, Brichova M, Heissigerova J, Rihova E, and Svozilkova P
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- Humans, Retrospective Studies, Czech Republic epidemiology, Syphilis complications, Syphilis drug therapy, Syphilis diagnosis, Papilledema complications, Coinfection complications, Uveitis drug therapy, Uveitis etiology, Uveitis diagnosis, Eye Infections, Bacterial drug therapy, Eye Infections, Bacterial diagnosis, Eye Infections, Bacterial epidemiology, Uveitis, Posterior complications, HIV Infections complications, HIV Infections diagnosis, HIV Infections drug therapy
- Abstract
Aims: To analyse the hallmarks of ocular manifestations of and therapeutic modalities for syphilis in the last two decades. The clinical features of syphilitic uveitis, and association with the human immunodeficiency virus (HIV) coinfection are described., Methods: Retrospective study of 16 patients diagnosed with ocular syphilis confirmed by serological tests in the General University Hospital in Prague between the years 2004 and 2021. General characteristics of ocular and systemic manifestations and visual functions were analysed., Results: An increasing incidence of syphilitic uveitis correlates with a general rise in syphilis cases. In our study, the ocular manifestation of syphilis was panuveitis (44%), posterior uveitis (31%) and anterior uveitis (25%). Posterior uveitis was found in 3 patients (19%) associated with preretinal infiltrates, that are often present in syphilitic uveitis. The worst visual outcomes were among patients with human immunodeficiency virus (HIV) coinfection and/or neurosyphilis, however the data were not significant. Optic disc edema was present in 56%, macular involvement in 37% of patients. Overall, 31% of patients in our cohort had persistent visual field defects due to impairment of their optic nerve or macula despite the final median Snellen visual acuity of 1.0. Two out of sixteen patients were treated with corticosteroids in addition to antibiotics., Conclusion: Posterior uveitis with preretinal infiltrates and optic disc edema should arouse suspicion of ocular syphilis. Recent data show the advantages of adjacent systemic corticosteroid treatment for severe forms of syphilitic uveitis and/or neuritis. Our observation supports this finding., Competing Interests: The authors report no conflicts of interest in this work.
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- 2023
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13. The Prevalence of MRI-Defined Sacroiliitis and Classification of Spondyloarthritis in Patients with Acute Anterior Uveitis: A Longitudinal Single-Centre Cohort Study.
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Bubova K, Hasikova L, Mintalova K, Gregova M, Kasalicky P, Klimova A, Brichova M, Svozilkova P, Heissigerova J, Vencovsky J, Pavelka K, and Senolt L
- Abstract
Background: Acute anterior uveitis (AAU) is a relatively common extra-musculoskeletal manifestation of axial spondyloarthritis (axSpA); however, data on the prevalence of active sacroiliitis in patients with AAU are limited., Methods: 102 patients with AAU and 39 healthy subjects (HS) underwent clinical assessment and sacroiliac joint MRI. Patients with absence of active sacroiliitis were reassessed after two years. International Spondyloarthritis Society (ASAS) classification criteria for axSpA (regardless of patient's age) and expert opinion for definitive diagnosis of axSpA were applied., Results: Although chronic back pain was equally present in both groups, bone marrow edema (BME) in SIJ and BME highly suggestive of axSpA was found in 52 (51%) and in 33 (32%) patients with AAU compared with 11 (28%) and none in HS, respectively. Out of all AAU patients, 41 (40%) patients fulfilled the ASAS classification criteria for axSpA, and 29 (28%) patients were considered highly suggestive of axSpA based on clinical features. Two out of the 55 sacroiliitis-negative patients developed active sacroiliitis at the two-year follow-up., Conclusions: One-third of patients with AAU had active inflammation on SIJ MRI and clinical diagnosis of axSpA. Therefore, patients with AAU, especially those with chronic back pain, should be referred to a rheumatologist, and the examination should be repeated if a new feature of SpA appears.
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- 2022
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14. Efficiency of 123 I-ioflupane SPECT as the marker of basal ganglia damage in acute methanol poisoning: 6-year prospective study.
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Kotikova K, Zogala D, Ptacnik V, Trnka J, Kupka K, Vaneckova M, Seidl Z, Diblik P, Heissigerova J, Navratil T, Komarc M, Zak I, Polakova K, Brozova H, and Zakharov S
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- Adult, Basal Ganglia diagnostic imaging, Basal Ganglia Diseases diagnostic imaging, Female, Humans, Iodine Radioisotopes, Longitudinal Studies, Magnetic Resonance Imaging, Male, Middle Aged, Neuroimaging, Nortropanes, Prospective Studies, Putamen diagnostic imaging, Putamen drug effects, Radiopharmaceuticals, Tomography, Emission-Computed, Single-Photon methods, Basal Ganglia drug effects, Basal Ganglia Diseases chemically induced, Methanol poisoning
- Abstract
Context: Investigate whether
123 I-ioflupane SPECT (DaT SPECT) has the potential as a marker of basal ganglia damage in acute methanol poisoning., Methods: Prospective, single-centre, cohort study of patients with confirmed methanol poisoning was conducted. DaT SPECT was performed twice with semi-quantification using DaTQUANTTM and MRI-based volumetry was calculated. Specific binding ratios (SBR) of striatum, caudate nucleus, and putamen were correlated with laboratory parameters of outcome, volumetric data, and retinal nerve fibres layer (RNFL) thickness measurements., Results: Forty-two patients (mean age 46.3 ± 4.2 years; 8 females), including 15 with MRI-detected putamen lesions (group I) and 27 patients with intact putamen (group II), underwent DaT SPECT. Volumetry was calculated in 35 of the patients assessed. SBR values for the left putamen correlated with putamen volume ( r = 0.665; p < 0.001). Decreased bilateral SBR values were determined for the striatum and the putamen, but not for the nucleus caudate, in group I ( p < 0.05). Significant correlation was observed between the SBR of the posterior putamen and arterial blood pH ( r = 0.574; p < 0.001) and other toxicological parameters of severity of poisoning/outcome including serum lactate, glucose, and creatinine concentrations ( p < 0.05). The SBR of the posterior putamen positively correlated with the global RNFL thickness ( p < 0.05). ROC analysis demonstrated a significant discriminatory ability of SBR of the posterior putamen with AUC = 0.753 (95%CI 0.604-0.902; p = 0.007). The multivariate regression model demonstrated that arterial blood pH, age, and gender were the most significant factors associated with SBR of the posterior putamen., Conclusion: DaT SPECT demonstrates significant potential for the diagnosis of methanol-induced basal ganglia damage.- Published
- 2021
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15. Immune Privilege: The Microbiome and Uveitis.
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Mölzer C, Heissigerova J, Wilson HM, Kuffova L, and Forrester JV
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- Animals, Bacteria metabolism, Blood-Brain Barrier immunology, Blood-Brain Barrier metabolism, Dysbiosis, Genetic Predisposition to Disease, HLA-B27 Antigen genetics, HLA-B27 Antigen immunology, Humans, Risk Factors, T-Lymphocytes, Regulatory immunology, T-Lymphocytes, Regulatory metabolism, T-Lymphocytes, Regulatory microbiology, Uvea metabolism, Uveitis genetics, Uveitis metabolism, Uveitis microbiology, Bacteria immunology, Gastrointestinal Microbiome, Immune Privilege, Intestines microbiology, Uvea immunology, Uveitis immunology
- Abstract
Immune privilege (IP), a term introduced to explain the unpredicted acceptance of allogeneic grafts by the eye and the brain, is considered a unique property of these tissues. However, immune responses are modified by the tissue in which they occur, most of which possess IP to some degree. The eye therefore displays a spectrum of IP because it comprises several tissues. IP as originally conceived can only apply to the retina as it contains few tissue-resident bone-marrow derived myeloid cells and is immunologically shielded by a sophisticated barrier - an inner vascular and an outer epithelial barrier at the retinal pigment epithelium. The vascular barrier comprises the vascular endothelium and the glia limitans. Immune cells do not cross the blood-retinal barrier (BRB) despite two-way transport of interstitial fluid, governed by tissue oncotic pressure. The BRB, and the blood-brain barrier (BBB) mature in the neonatal period under signals from the expanding microbiome and by 18 months are fully established. However, the adult eye is susceptible to intraocular inflammation (uveitis; frequency ~200/100,000 population). Uveitis involving the retinal parenchyma (posterior uveitis, PU) breaches IP, while IP is essentially irrelevant in inflammation involving the ocular chambers, uveal tract and ocular coats (anterior/intermediate uveitis/sclerouveitis, AU). Infections cause ~50% cases of AU and PU but infection may also underlie the pathogenesis of immune-mediated "non-infectious" uveitis. Dysbiosis accompanies the commonest form, HLA-B27-associated AU, while latent infections underlie BRB breakdown in PU. This review considers the pathogenesis of uveitis in the context of IP, infection, environment, and the microbiome., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Mölzer, Heissigerova, Wilson, Kuffova and Forrester.)
- Published
- 2021
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16. Severity of Experimental Autoimmune Uveitis Is Reduced by Pretreatment with Live Probiotic Escherichia coli Nissle 1917.
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Dusek O, Fajstova A, Klimova A, Svozilkova P, Hrncir T, Kverka M, Coufal S, Slemin J, Tlaskalova-Hogenova H, Forrester JV, and Heissigerova J
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- Animals, Disease Models, Animal, Female, Intestinal Mucosa pathology, Mice, Mice, Inbred C57BL, Autoimmune Diseases therapy, Escherichia coli, Inflammation therapy, Probiotics administration & dosage, Probiotics pharmacology, Uveitis therapy
- Abstract
Non-infectious uveitis is considered an autoimmune disease responsible for a significant burden of blindness in developed countries and recent studies have linked its pathogenesis to dysregulation of the gut microbiota. We tested the immunomodulatory properties of two probiotics, Escherichia coli Nissle 1917 (EcN) and E. coli O83:K24:H31 (EcO), in a model of experimental autoimmune uveitis (EAU). To determine the importance of bacterial viability and treatment timing, mice were orally treated with live or autoclaved bacteria in both preventive and therapeutic schedules. Disease severity was assessed by ophthalmoscopy and histology, immune phenotypes in mesenteric and cervical lymph nodes were analyzed by flow cytometry and the gut immune environment was analyzed by RT-PCR and/or gut tissue culture. EcN, but not EcO, protected against EAU but only as a live organism and only when administered before or at the time of disease induction. Successful prevention of EAU was accompanied by a decrease in IRBP-specific T cell response in the lymph nodes draining the site of immunization as early as 7 days after the immunization and eye-draining cervical lymph nodes when the eye inflammation became apparent. Furthermore, EcN promoted an anti-inflammatory response in Peyer's patches, increased gut antimicrobial peptide expression and decreased production of inducible nitric oxide synthase in macrophages. In summary, we show here that EcN controls inflammation in EAU and suggest that probiotics may have a role in regulating the gut-eye axis.
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- 2020
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17. Infectious uveitis in immunodeficient HIV-negative patients: A retrospective study.
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Svozilkova P, Rihova E, Brichova M, Havlikova A, Klimova A, and Heissigerova J
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- Adolescent, Adult, Aged, Aged, 80 and over, Czech Republic, Eye Infections, Viral physiopathology, Female, Humans, Male, Middle Aged, Retrospective Studies, Uveitis physiopathology, Young Adult, Eye Infections, Viral diagnosis, Eye Infections, Viral etiology, Eye Infections, Viral therapy, Immunocompromised Host, Uveitis diagnosis, Uveitis etiology, Uveitis therapy
- Abstract
Aims: Cases of infectious uveitis in immunodeficient patients may present with atypical clinical features because the clinical course of disease is usually affected by the compromised immune system. Therefore, it is sometimes difficult to determine the correct diagnosis. The aim of this study was to evaluate a prevalence of immunodeficient HIV-negative individuals among patients with infectious uveitis and to assess diagnostic and therapeutic approaches., Methods: A retrospective study., Results: In years 2003-2017, we diagnosed 594 patients with infectious uveitis. In 35 of them, infectious uveitis occurred on the basis of immunodeficiency (malignancy, immunosuppressive therapy etc.). The most common infectious uveitis was cytomegalovirus retinitis, followed by acute retinal necrosis, herpetic anterior uveitis, endogenous fungal endophthalmitis, toxoplasmic retinochoroiditis, progressive outer retinal necrosis and syphilis. In indicated cases, intraocular fluid examination was a valuable diagnostic tool. After initiation of treatment, improvement of BCVA was observed in 13 eyes, stabilization in 13 eyes and deterioration in 11 eyes. In some patients who experienced adverse effects of medication, the recommended doses were decreased., Conclusion: Our experience shows that patients with diagnoses of acute retinal necrosis or progressive outer retinal necrosis have a poor visual prognosis. The occurrence of cytomegalovirus retinitis signifies a very unfavorable survival prognosis in patients who underwent hematopoietic stem cell transplantation; the patients in our study died within 1 year from cytomegalovirus retinitis diagnosis.
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- 2020
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18. Health-related quality of life determinants in survivors of a mass methanol poisoning outbreak: six-year prospective cohort study.
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Rulisek J, Waldauf P, Belohlavek J, Balik M, Kotikova K, Hlusicka J, Vaneckova M, Seidl Z, Diblik P, Bydzovsky J, Heissigerova J, Urban P, Miovsky M, Sejvl J, Pelclova D, and Zakharov S
- Subjects
- Adult, Alcoholism complications, Brain diagnostic imaging, Cohort Studies, Ethanol administration & dosage, Evoked Potentials, Visual, Female, Follow-Up Studies, Fomepizole administration & dosage, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Prospective Studies, Retina pathology, Surveys and Questionnaires, Survivors, Antidotes administration & dosage, Disease Outbreaks, Methanol poisoning, Quality of Life
- Abstract
Purpose: The effect of acute methanol poisoning on the follow-up quality of life of survivors in mass poisoning outbreaks is not known. The objective of this is to study the impact of visual and central nervous system (CNS) sequelae of methanol poisoning on long-term health-related quality of life (QoL) of survivors, its clinical determinants, and dynamics. Materials and methods: A total of 54 patients with confirmed methanol poisoning (mean age 46.7 ± 13.4 years, 9 females) were examined consequently three times within six-year prospective cohort study and compared to 23 controls with the history of chronic alcohol abuse. The following tests were performed: SF-36 QoL questionnaire, visual evoked potentials (VEP) of optic nerve, ocular examination with retinal nerve fiber layer (RNFL) thickness measurement, brain magnetic resonance imaging (MRI), and biochemical and toxicological tests. Results: Acute methanol poisoning led to significant decrease in physical component summary (PCS) compared to PCS of age-adjusted controls (mean score with SD 46.8 ± 11.0 versus 52.3 ± 9.4 points; p = .003). In 17/40 (42.5%) patients with three rounds of examination, signs of severe disability (≤30 points in at least one score) were present six years after discharge, with negative dynamics of PCS score during the observation period. The patients with abnormal RNFL thickness had lower PCS (mean difference 10.5 points; 95%CI 3.5-17.5, p = .004) and mental component summary score (9.5 points; 95%CI 1.9-17.1, p = .015) compared to the patients with normal RNFL. Signs of physical and mental adaptation to long-term visual sequelae were registered with gradual reduction of difference in most of physical and mental components scores compared to the patients with normal RNFL during six years of observation. Signs of hemorrhagic brain lesions were associated with permanent decrease of PCS score (mean difference 7.4 points; 95%CI 0.6-14.0; p = .033), bodily pain (8.7 points; 95%CI 1.6-17.6; p = .018), and social functioning (8.2 points; 95%CI 3.0-17.4; p = .005) six years after discharge. No effect of type of antidote (fomepizole versus ethanol) and extracorporeal enhanced elimination modality (intermittent hemodialysis versus continuous renal replacement therapy) applied in hospital on long-term QoL was found (all p > .05). Conclusion: Acute methanol poisoning was associated with a significant decrease of health-related quality of life of survivors persisting for at least six years after discharge. The more pronounced decrease in QoL scores was observed in the patients with hemorrhagic brain lesions and visual sequelae of poisoning with abnormal RNFL thickness.
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- 2020
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19. The impact of co-morbidities on a 6-year survival after methanol mass poisoning outbreak: possible role of metabolic formaldehyde.
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Zakharov S, Rulisek J, Hlusicka J, Kotikova K, Navratil T, Komarc M, Vaneckova M, Seidl Z, Diblik P, Bydzovsky J, Heissigerova J, Zogala D, Hubacek JA, Miovsky M, Sejvl J, Vojtova L, and Pelclova D
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- Adolescent, Adult, Cohort Studies, Disease Outbreaks statistics & numerical data, Female, Follow-Up Studies, Formaldehyde metabolism, Humans, Longitudinal Studies, Male, Methanol pharmacokinetics, Middle Aged, Poisoning epidemiology, Prospective Studies, Risk Factors, Survival Rate, Young Adult, Formaldehyde poisoning, Hospital Mortality, Hospitalization statistics & numerical data, Methanol poisoning, Poisoning mortality
- Abstract
Context: The influence of co-morbid conditions on the outcome of acute methanol poisoning in mass poisoning outbreaks is not known. Objective: The objective of this is to study the impact of burden of co-morbidities, complications, and methanol-induced brain lesions on hospital, follow-up, and total mortality. Methods: All patients hospitalized with methanol poisoning during a mass poisoning outbreak were followed in a prospective cohort study until death or final follow-up after 6 years. The age-adjusted Charlson co-morbidity index (ACCI) score was calculated for each patient. A multivariate Cox regression model was used to calculate the adjusted hazards ratio (HR) for death. The survival was modeled using the Kaplan-Meier method. Results: Of 108 patients (mean age with SD 50.9 ± 2.6 years), 24 (54.4 ± 5.9 years) died during hospitalization (mean survival with SD 8 ± 4 days) and 84 (49.9 ± 3.0 years; p = .159) were discharged, including 27 with methanol-induced brain lesions. Of the discharged patients, 15 (56.3 ± 6.8 years) died during the follow-up (mean survival 37 ± 11 months) and 69 (48.5 ± 3.3 years; p = .044) survived. The hospital mortality was 22%, the follow-up mortality was 18%; the total mortality was 36%. Cardiac/respiratory arrest, acute respiratory failure, multiorgan failure syndrome, and arterial hypotension increased the HR for hospital and total (but not follow-up) mortality after adjustment for age, sex, and arterial pH (all p < .05). All patients who died in the hospital had at least one complication. A higher ACCI score was associated with greater total mortality (HR 1.22; 1.00-1.48 95% CI; p = .046). Of those who died, 35 (90%) had a moderate-to-high ACCI. The Kaplan-Meier curve demonstrated that patients with a high ACCI had greater follow-up mortality compared to ones with low ( p = .027) or moderate ( p = .020) scores. For the patients who died during follow-up, cancers of different localizations were responsible for 7/15 (47%) of the deaths. Conclusions: The character and number of complications affected hospital but not follow-up mortality, while the burden of co-morbidities affected follow-up mortality. Methanol-induced brain lesions did not affect follow-up mortality. Relatively high cancer mortality rate may be associated with acute exposure to metabolic formaldehyde produced by methanol oxidation.
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- 2020
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20. Clinical and genetic determinants of chronic visual pathway changes after methanol - induced optic neuropathy: four-year follow-up study.
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Nurieva O, Hubacek JA, Urban P, Hlusicka J, Diblik P, Kuthan P, Sklenka P, Meliska M, Bydzovsky J, Heissigerova J, Kotikova K, Navratil T, Komarc M, Seidl Z, Vaneckova M, Vojtova L, and Zakharov S
- Subjects
- Adult, Case-Control Studies, Czech Republic, Evoked Potentials, Visual, Female, Follow-Up Studies, Gene Frequency, Genetic Predisposition to Disease, Humans, Longitudinal Studies, Male, Middle Aged, Optic Nerve physiopathology, Optic Nerve Diseases diagnosis, Optic Nerve Diseases genetics, Optic Nerve Diseases physiopathology, Prognosis, Prospective Studies, Reaction Time, Risk Factors, Time Factors, Vision Disorders diagnosis, Vision Disorders genetics, Vision Disorders physiopathology, Vision, Ocular genetics, Apolipoprotein E4 genetics, Methanol poisoning, Optic Nerve drug effects, Optic Nerve Diseases chemically induced, Vision Disorders chemically induced, Vision, Ocular drug effects
- Abstract
Context: Methanol poisoning induces acute optic neuropathy with possible long-term visual damage., Objective: To study the dynamics and key determinants of visual pathway functional changes during 4 years after acute methanol poisoning., Methods: A total of 42 patients with confirmed methanol poisoning (mean age 45.7 ± 4.4 years) were examined 4.9 ± 0.6, 25.0 ± 0.6, and 49.9 ± 0.5 months after discharge. The following tests were performed: visual evoked potential (VEP), retinal nerve fiber layer (RNFL) measurement, brain magnetic resonance imaging (MRI), complete ocular examination, biochemical tests, and apolipoprotein E (ApoE) genotyping., Results: Abnormal VEP P1 latency was registered in 18/42 right eyes (OD) and 21/42 left eyes (OS), abnormal N1P1 amplitude in 10/42 OD and OS. Mean P1 latency shortening during the follow-up was 15.0 ± 2.0 ms for 36/42 (86%) OD and 14.9 ± 2.4 ms for 35/42 (83%) OS, with maximum shortening up to 35.0 ms. No significant change of mean N1P1 amplitude was registered during follow-up. A further decrease in N1P1 amplitude ≥1.0 mcV in at least one eye was observed in 17 of 36 patients (47%) with measurable amplitude (mean decrease -1.11 ± 0.83 (OD)/-2.37 ± 0.66 (OS) mcV versus -0.06 ± 0.56 (OD)/-0.83 ± 0.64 (OS) mcV in the study population; both p < .001). ApoE4 allele carriers had lower global and temporal RNFL thickness and longer initial P1 latency compared to the non-carriers (all p < .05). The odds ratio for abnormal visual function was 8.92 (3.00-36.50; 95%CI) for ApoE4 allele carriers (p < .001). The presence of ApoE4 allele was further associated with brain necrotic lesions (r = 0.384; p = .013) and brain hemorrhages (r = 0.395; p = .011)., Conclusions: Improvement of optic nerve conductivity occurred in more than 80% of patients, but evoked potential amplitude tended to decrease during the 4 years of observation. ApoE4 allele carriers demonstrated lower RNFL thickness, longer P1 latency, and more frequent methanol-induced brain damage compared to non-carriers.
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- 2019
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21. Neuroinflammation markers and methyl alcohol induced toxic brain damage.
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Zakharov S, Hlusicka J, Nurieva O, Kotikova K, Lischkova L, Kacer P, Kacerova T, Urban P, Vaneckova M, Seidl Z, Diblik P, Kuthan P, Heissigerova J, Lesovsky J, Rulisek J, Vojtova L, Hubacek JA, and Navratil T
- Subjects
- Biomarkers blood, Brain diagnostic imaging, Brain drug effects, Brain physiopathology, Evoked Potentials, Visual drug effects, Female, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Neurotoxicity Syndromes diagnosis, Neurotoxicity Syndromes mortality, Neurotoxicity Syndromes physiopathology, Prospective Studies, Reaction Time drug effects, Time Factors, Cytokines blood, Inflammation Mediators blood, Methanol poisoning, Neurotoxicity Syndromes blood
- Abstract
Methyl alcohol intoxication is a global problem with high mortality and long-term visual sequelae and severe brain damage in survivors. The role of neuroinflammation in the mechanisms of methyl alcohol-induced toxic brain damage has not been well studied. We measured the acute concentrations and dynamics of lipoxins LxA4 and LxB4 and the interleukins IL-4, IL-5, IL-9, IL-10, and IL-13 in the serum of patients treated with methyl alcohol poisoning and the follow-up concentrations in survivors two years after discharge from the hospital. A series of acute measurements was performed in 28 hospitalized patients (mean age 54.2 ± 5.2 years, mean observation time 88 ± 20 h) and the follow-up measurements were performed in 36 subjects who survived poisoning (including 12/28 survivors from the acute group). Visual evoked potentials (VEP) and magnetic resonance imaging of the brain (MRI) were performed to detect long-term visual and brain sequelae of intoxication. The acute concentrations of inflammatory mediators were higher than the follow-up concentrations: LxA4, 62.0 ± 6.0 vs. 30.0 ± 5.0 pg/mL; LxB4, 64.0 ± 7.0 vs. 34.0 ± 4.0 pg/mL; IL-4, 29.0 ± 4.0 vs. 15.0 ± 1.0 pg/mL; IL-5, 30.0 ± 4.0 vs. 13.0 ± 1.0 pg/mL; IL-9, 30.0 ± 4.0 vs. 13.0 ± 1.0 pg/mL; IL-10, 38.0 ± 5.0 vs. 16.0 ± 1.0 pg/mL; IL-13, 35.0 ± 4.0 vs. 14.0 ± 1.0 pg/mL (all p < 0.001). The patients with higher follow-up IL-5 concentration had prolonged latency P1 (r = 0.413; p = 0.033) and lower amplitude N1P1 (r = -0.498; p = 0.010) of VEP. The higher follow-up IL-10 concentration was associated with MRI signs of brain necrotic damage (r = 0.533; p = 0.001) and brain hemorrhage (r = 0.396; p = 0.020). Our findings suggest that neuroinflammation plays an important role in the mechanisms of toxic brain damage in acute methyl alcohol intoxication., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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22. Reply.
- Author
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Zakharov S, Nurieva O, Diblik P, Kuthan P, Sklenka P, Meliska M, Bydzovsky J, Heissigerova J, Urban P, Kotikova K, Navratil T, Komarc M, Seidl Z, Vaneckova M, and Pelclova D
- Subjects
- Humans, Methanol, Prospective Studies, Optic Nerve Diseases, Optic Neuritis
- Published
- 2018
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23. Combined treatment of primary vitreoretinal lymphomas significantly prolongs the time to first relapse.
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Klimova A, Heissigerova J, Rihova E, Brichova M, Pytlik R, Spicka I, Mrazova K, Karolova J, and Svozilkova P
- Subjects
- Aged, Disease-Free Survival, Eye Neoplasms drug therapy, Eye Neoplasms mortality, Eye Neoplasms physiopathology, Female, Flow Cytometry, Humans, Intraocular Lymphoma mortality, Intraocular Lymphoma physiopathology, Intravitreal Injections, Male, Methotrexate administration & dosage, Middle Aged, Procarbazine administration & dosage, Prognosis, Retinal Neoplasms mortality, Retinal Neoplasms physiopathology, Retrospective Studies, Rituximab administration & dosage, Survival Rate, Time Factors, Vincristine administration & dosage, Visual Acuity physiology, Vitreous Body pathology, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Intraocular Lymphoma drug therapy, Neoplasm Recurrence, Local physiopathology, Retinal Neoplasms drug therapy, Vitreous Body drug effects
- Abstract
Background: Vitreoretinal lymphomas belong to the family of central nervous system (CNS) lymphomas. The optimal approach for the treatment of isolated primary vitreoretinal lymphoma is unclear because of the lack of large comparative clinical series. Combination of intravitreal and systemic chemotherapy is recommended in many reports. The aim of our retrospective study was to compare the survival rate and prognosis of patients with vitreoretinal lymphoma with and without CNS involvement., Methods: Twenty patients with vitreoretinal lymphomas were observed between the years 2004and2016, 10 patients with primary vitreoretinal lymphoma and 10 with primary CNS lymphoma. To compare survival rates, we included 53 patients diagnosed with primary CNS lymphoma without vitreoretinal involvement between the years 2002and2011 from our haemato-oncology department., Results: The 5-year survival rate was estimated 71% in patients with vitreoretinal lymphoma in our observation. Significantly longer 5-year overall survival (P˂0.01) was observed in patients with vitreoretinal lymphoma compared with patients with primary CNS lymphoma without vitreoretinal involvement. Progression-free survival was almost equal in both groups of patients with primary vitreoretinal lymphoma and primary CNS lymphoma (P=0.363). The relapse of lymphoma was frequent (50%-60%) with the median time to first relapse of 31 months. Combined treatment (local and systemic) in patients without CNS involvement significantly prolonged progression-free survival in our study (P˂0.05)., Conclusion: Combined treatment of primary vitreoretinal lymphoma significantly delays the relapse of lymphoma compared with local therapy alone. Intraocular involvement brings significant positive prognostic value when overall survival is compared., Competing Interests: Competing interests: None declared., (© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.)
- Published
- 2018
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24. Progressive Chronic Retinal Axonal Loss Following Acute Methanol-induced Optic Neuropathy: Four-Year Prospective Cohort Study.
- Author
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Nurieva O, Diblik P, Kuthan P, Sklenka P, Meliska M, Bydzovsky J, Heissigerova J, Urban P, Kotikova K, Navratil T, Komarc M, Seidl Z, Vaneckova M, Pelclova D, and Zakharov S
- Subjects
- Acute Disease, Axons drug effects, Brain pathology, Chronic Disease, Disease Progression, Female, Follow-Up Studies, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Optic Disk drug effects, Optic Nerve Diseases chemically induced, Optic Nerve Diseases physiopathology, Prospective Studies, Retinal Ganglion Cells drug effects, Retinal Neurons drug effects, Solvents poisoning, Time Factors, Tomography, Optical Coherence methods, Tomography, X-Ray Computed, Visual Acuity, Visual Fields, Axons pathology, Methanol poisoning, Optic Disk pathology, Optic Nerve Diseases diagnosis, Retinal Ganglion Cells pathology, Retinal Neurons pathology
- Abstract
Purpose: To study the dynamics and clinical determinants of chronic retinal nerve fiber layer thickness (RNFL) loss after methanol-induced optic neuropathy., Design: Prospective cohort study., Methods: All patients underwent complete ophthalmic evaluation including spectral-domain optical coherence tomography 3 times during 4 years of observation: 4.9 (±0.6), 25.0 (±0.6), and 49.9 (±0.5) months after discharge., Participants: Eighty-four eyes of 42 survivors of methanol poisoning, mean age (standard deviation) of 45.7 (±4.4) years; and 82 eyes of 41 controls, mean age 44.0 (±4.2) years., Main Outcome Measures: Global and temporal RNFL loss., Results: Abnormal RNFL thickness was registered in 13 of 42 (31%) survivors of methanol poisoning and chronic axonal loss in 10 of 42 (24%) patients. Significant decrease of global/temporal RNFL thickness during the observation period was found in the study population compared to the controls (P < .001). The risk estimate of chronic global RNFL loss for arterial blood pH < 7.3 at admission was 11.65 (95% confidence interval 1.91-71.12) after adjusting for age and sex. The patients with chronic axonal degeneration demonstrated progressive visual loss in 7 of 10 cases. The patients with abnormal RNFL thickness had magnetic resonance signs of brain damage in 10 of 13 vs 8 of 29 cases with normal RNFL thickness (P = .003). Signs of brain hemorrhages were present in 7 of 13 patients with abnormal RNFL thickness vs 5 of 29 cases with normal RNFL thickness (P = .015)., Conclusions: Methanol-induced optic neuropathy may lead to chronic retinal axonal loss during the following years. Arterial blood pH on admission is the strongest predictor of chronic RNFL thickness decrease. Chronic retinal neurodegeneration is associated with the progressive loss of visual functions and necrotic brain lesions., (Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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25. The critical points in induction of experimental autoimmune uveitis.
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Klimova A, Seidler Stangova P, Svozilkova P, Forrester JV, Klaska I, and Heissigerova J
- Subjects
- Adjuvants, Immunologic, Animals, Chronic Disease, Female, Freund's Adjuvant, Irritants, Mice, Inbred C57BL, Pertussis Toxin, Autoimmune Diseases chemically induced, Disease Models, Animal, Eye Proteins, Retinol-Binding Proteins, Uveitis chemically induced
- Abstract
Background: Autoimmune uveitis is a leading cause of visual impairment in developed countries in patients of working age. Animal models of experimental autoimmune uveitis (EAU) have been established to serve as a useful template for novel therapeutic approaches., Methods: Experimental autoimmune uveitis is induced in C57BL/6 mice by subcutaneous application of interphotoreceptor retinoid binding protein in complete Freund's adjuvant and pertussis toxin. Clinical and histological grading is used to assess the inflammation intensity of EAU., Results: The protocol of induction of EAU in mice hides several important aspects, which are crucial for developing the disease. These details have to be addressed to ensure reproducible disease induction. We describe our experience in establishing the model by pointing out the critical steps in EAU protocol which we found important., Conclusion: The mouse model of EAU has practical value for preclinical studies, is robust and well established. However, the induction of inflammation of the eye can be quite challenging when important details of the protocol are not recognized and adhered to.
- Published
- 2016
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26. The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis.
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Heissigerova J, Seidler Stangova P, Klimova A, Svozilkova P, Hrncir T, Stepankova R, Kverka M, Tlaskalova-Hogenova H, and Forrester JV
- Subjects
- Adaptive Immunity, Animals, Anti-Bacterial Agents pharmacology, Autoantigens immunology, Autoimmune Diseases chemically induced, Autoimmune Diseases microbiology, Bacterial Load drug effects, Disease Models, Animal, Eye pathology, Eye Proteins immunology, Female, Flow Cytometry, Germ-Free Life, Interferon-gamma biosynthesis, Interleukin-17 biosynthesis, Lymphocyte Activation, Macrophages immunology, Male, Mice, Mice, Inbred C57BL, Retina immunology, Retinitis chemically induced, Retinitis etiology, Retinitis microbiology, Retinol-Binding Proteins immunology, T-Lymphocytes, Regulatory immunology, Uveitis chemically induced, Uveitis immunology, Autoimmune Diseases immunology, Eye immunology, Microbiota immunology, Retinitis immunology, Uveitis microbiology
- Abstract
The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). We induced EAU in germ-free (GF) or conventionally housed (CV) mice and in CV mice treated with a combination of broad-spectrum antibiotics either from the day of EAU induction or from one week prior to induction of disease. The severity of the inflammation was assessed by fundus biomicroscopy or by histology, including immunohistology. The immunophenotyping of T cells in local and distant lymph nodes was performed by flow cytometry. We found that GF mice and mice where the microbiota was reduced one week before EAU induction were protected from severe autoimmune inflammation. GF mice had lower numbers of infiltrating macrophages and significantly less T cell infiltration in the retina than CV mice with EAU. GF mice also had reduced numbers of IFN-γ and IL-17-producing T cells and increased numbers of regulatory T cells in the eye-draining lymph nodes. These data suggest that the presence of microbiota during autoantigen recognition regulates the inflammatory response by influencing the adaptive immune response.
- Published
- 2016
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27. Tumor necrosis factor polymorphisms associated with tumor necrosis factor production influence the risk of idiopathic intermediate uveitis.
- Author
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Atan D, Heissigerova J, Kuffová L, Hogan A, Kilmartin DJ, Forrester JV, Bidwell JL, Dick AD, and Churchill AJ
- Subjects
- Adult, Aged, Aged, 80 and over, Case-Control Studies, Female, Gene Frequency, Genetic Association Studies, Genetic Predisposition to Disease, HLA-DRB1 Chains genetics, Haplotypes, Humans, Interleukin-10 genetics, Male, Middle Aged, Multiple Sclerosis genetics, Multiple Sclerosis immunology, Receptors, Tumor Necrosis Factor, Type I genetics, Risk Factors, Uveitis, Intermediate immunology, Young Adult, Polymorphism, Genetic, Tumor Necrosis Factor-alpha biosynthesis, Tumor Necrosis Factor-alpha genetics, Uveitis, Intermediate etiology, Uveitis, Intermediate genetics
- Abstract
Purpose: Idiopathic intermediate uveitis (IIU) is a potentially sight-threatening inflammatory disorder with well-defined anatomic diagnostic criteria. It is often associated with multiple sclerosis, and both conditions are linked to HLA-DRB1*15. Previously, we have shown that non-infectious uveitis (NIU) is associated with interleukin 10 (IL10) polymorphisms, IL10-2849A (rs6703630), IL10+434T (rs2222202), and IL10+504G (rs3024490), while a LTA+252AA/TNFA-238GG haplotype (rs909253/rs361525) is protective. In this study, we determined whether patients with IIU have a similar genetic profile as patients with NIU or multiple sclerosis., Methods: Twelve polymorphisms were genotyped, spanning the tumor necrosis factor (TNF) and IL10 genomic regions, in 44 patients with IIU and 92 population controls from the UK and the Republic of Ireland., Results: IIU was strongly associated with the TNFA-308A and TNFA-238A polymorphisms. We found the combination of TNFA-308 and -238 loci was more strongly associated with IIU than any other loci across the major histocompatibility complex, including HLA-DRB1., Conclusions: TNF polymorphisms, associated with increased TNF production, are highly associated with IIU. These results offer the potential to ascribe therapeutic response and risk (i.e., the influence of HLA-DRB1*15 status and TNFR1 polymorphism) to anti-TNF therapy in IIU.
- Published
- 2013
28. A randomized trial of tacrolimus versus tacrolimus and prednisone for the maintenance of disease remission in noninfectious uveitis.
- Author
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Lee RW, Greenwood R, Taylor H, Amer R, Biester S, Heissigerova J, Forrester JV, and Dick AD
- Subjects
- Administration, Oral, Adult, Drug Therapy, Combination, Female, Glucocorticoids adverse effects, Humans, Immunosuppressive Agents adverse effects, Maintenance Chemotherapy, Male, Prednisone adverse effects, Remission Induction, Tacrolimus adverse effects, Treatment Outcome, Uveitis physiopathology, Visual Acuity physiology, Glucocorticoids therapeutic use, Immunosuppressive Agents therapeutic use, Prednisone therapeutic use, Tacrolimus therapeutic use, Uveitis drug therapy
- Abstract
Purpose: To compare tacrolimus monotherapy with tacrolimus and prednisone therapy for the maintenance of disease remission in subjects with noninfectious posterior segment intraocular inflammation (PSII)., Design: Randomized, controlled, phase 2b, open-label, dual-center noninferiority trial., Participants: Fifty-eight patients with sight-threatening PSII., Methods: Patients requiring a second-line systemic immunosuppressive agent to control their PSII were treated with therapeutic doses of oral tacrolimus. Those subjects who subsequently were able to taper their prednisone dose to 10 mg daily without disease reactivation were assigned randomly either to stop prednisone or to continue 7.5 to 10 mg prednisone daily for 9 months., Main Outcome Measures: Change in logarithm of the minimum angle of resolution (logMAR) visual acuity (VA) and rate of patient withdrawal resulting from treatment inefficacy or intolerance., Results: Thirty-five patients successfully tapered their prednisone to 10 mg daily. Of these, 16 were allocated randomly to receive tacrolimus monotherapy and 19 to continue taking prednisone and tacrolimus dual therapy. The difference in the mean change in VA for monotherapy compared with the dual therapy group was less than 1 logMAR letter (logMAR, -0.008; 95% confidence interval, -0.108 to 0.092; P = 0.870). The proportion of patients who tolerated treatment and maintained disease remission for 9 months after randomization also was similar in both groups (monotherapy, 62.5%; dual therapy, 68.4%; P = 0.694). All monotherapy treatment failures were the result of disease reactivation, whereas 50% of dual-therapy failures were the result of drug intolerance., Conclusions: This study provides preliminary evidence that corticosteroids can be withdrawn in tacrolimus-treated patients who are able to achieve control of PSII with 10 mg prednisone daily, and any advantage of dual therapy in the prevention of disease reactivation was offset by its greater treatment intolerance. These findings support the further evaluation of corticosteroid-free treatment in future phase 3 trials (International Standard Randomised Controlled Trial Number Register identification, ISRCTN46576063)., Financial Disclosure(s): Proprietary or commercial disclosure may be found after the references., (Copyright © 2012 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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29. The role of pars plana vitrectomy in the diagnosis and treatment of uveitis.
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Svozilkova P, Heissigerova J, Brichova M, Kalvodova B, Dvorak J, and Rihova E
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents administration & dosage, Antiprotozoal Agents administration & dosage, Antiviral Agents administration & dosage, Child, Coloring Agents, Female, Flow Cytometry, Fluorescein Angiography, Humans, Indocyanine Green, Male, Middle Aged, Polymerase Chain Reaction, Retrospective Studies, Visual Acuity physiology, Vitreous Body immunology, Vitreous Body microbiology, Young Adult, Eye Infections diagnosis, Eye Infections drug therapy, Uveitis diagnosis, Uveitis drug therapy, Vitrectomy, Vitreous Body pathology
- Abstract
Purpose: To assess the diagnostic yield of vitreous fluid analysis and the therapeutic effect of pars plana vitrectomy in patients with suspected intraocular inflammation., Methods: During 2004-2008, pars plana vitrectomy was performed in 89 patients (101 eyes) out of 1233 patients with uveitis. Vitreous specimens were analyzed by cytologic and histopathologic examination, microbiologic culture, polymerase chain reaction, antibody determination, and flow cytometry. Vitrectomy was performed in 85 eyes for both diagnostic and therapeutic purposes; the remaining 16 eyes underwent only diagnostic pars plana vitrectomy. Preoperative and postoperative best-corrected Snellen visual acuity was compared., Results: Preoperative diagnoses were infection in 40 patients, malignant masquerade syndrome in 10 patients, and idiopathic uveitis in 39 patients. Vitreous analysis contributed to the determination of diagnosis in 54 patients (61%). Final diagnoses were infection in 42 patients, malignant masquerade syndrome in 6 patients, benign masquerade syndrome in 16 patients, and idiopathic uveitis in 25 patients. Vitreous fluid collected from each eye underwent approximately 2.2 (range 1-6) laboratory tests. Therapeutic reasons for vitrectomy were the treatment of complications of uveitis in 42 eyes and/or intravitreal application of anti-infectious or cytostatic drugs in 49 eyes. Overall, the visual acuity improved in 45% of eyes, remained unchanged in 45% of eyes, and decreased in 10% of eyes in 3 months after surgery., Conclusions: Pars plana vitrectomy with carefully selected testing is a valuable tool for assessment of diagnosis in a large proportion of patients with uveitis. Moreover, the therapeutic effect of vitrectomy can improve the visual outcomes in these patients.
- Published
- 2011
- Full Text
- View/download PDF
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