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The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis.
- Source :
-
Journal of immunology research [J Immunol Res] 2016; Vol. 2016, pp. 5065703. Date of Electronic Publication: 2016 May 17. - Publication Year :
- 2016
-
Abstract
- The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). We induced EAU in germ-free (GF) or conventionally housed (CV) mice and in CV mice treated with a combination of broad-spectrum antibiotics either from the day of EAU induction or from one week prior to induction of disease. The severity of the inflammation was assessed by fundus biomicroscopy or by histology, including immunohistology. The immunophenotyping of T cells in local and distant lymph nodes was performed by flow cytometry. We found that GF mice and mice where the microbiota was reduced one week before EAU induction were protected from severe autoimmune inflammation. GF mice had lower numbers of infiltrating macrophages and significantly less T cell infiltration in the retina than CV mice with EAU. GF mice also had reduced numbers of IFN-γ and IL-17-producing T cells and increased numbers of regulatory T cells in the eye-draining lymph nodes. These data suggest that the presence of microbiota during autoantigen recognition regulates the inflammatory response by influencing the adaptive immune response.
- Subjects :
- Adaptive Immunity
Animals
Anti-Bacterial Agents pharmacology
Autoantigens immunology
Autoimmune Diseases chemically induced
Autoimmune Diseases microbiology
Bacterial Load drug effects
Disease Models, Animal
Eye pathology
Eye Proteins immunology
Female
Flow Cytometry
Germ-Free Life
Interferon-gamma biosynthesis
Interleukin-17 biosynthesis
Lymphocyte Activation
Macrophages immunology
Male
Mice
Mice, Inbred C57BL
Retina immunology
Retinitis chemically induced
Retinitis etiology
Retinitis microbiology
Retinol-Binding Proteins immunology
T-Lymphocytes, Regulatory immunology
Uveitis chemically induced
Uveitis immunology
Autoimmune Diseases immunology
Eye immunology
Microbiota immunology
Retinitis immunology
Uveitis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 2314-7156
- Volume :
- 2016
- Database :
- MEDLINE
- Journal :
- Journal of immunology research
- Publication Type :
- Academic Journal
- Accession number :
- 27294159
- Full Text :
- https://doi.org/10.1155/2016/5065703