47 results on '"Han-chun Gao"'
Search Results
2. Human Bocavirus Infection, People’s Republic of China
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Xiao-Wang Qu, Zhao-jun Duan, Zheng-Yu Qi, Zhi-ping Xie, Han-chun Gao, Wen-Pei Liu, Can-Ping Huang, Fu-Wang Peng, Li-Shu Zheng, and Yun-de Hou
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human bocavirus ,China ,children ,dispatch ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
A newly identified parvovirus, human bocavirus (HBoV), was found in 21 (8.3%) of 252 nasopharyngeal aspirates from hospitalized children with lower respiratory tract infection in Hunan Province, People’s Republic of China. Viral loads were 104 to 1010 copies/mL. Phylogenetic analysis of the VP1 gene showed a single genetic lineage of HBoV worldwide.
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- 2007
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3. Development and biological activity of long-acting recombinant human interferon-α2b
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Luyan Zhu, Fenlian Ma, Chao Wang, Qian Zhang, Lihong Yao, Li-shu Zheng, and Han-chun Gao
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Interferon α2b ,Hepatitis B virus ,Glycosylation ,Recombinant Fusion Proteins ,lcsh:Biotechnology ,Antineoplastic Agents ,CHO Cells ,Biology ,Interferon alpha-2 ,Protein Engineering ,Antiviral Agents ,Chromatography, Affinity ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cricetulus ,Affinity chromatography ,law ,Interferon ,In vivo ,lcsh:TP248.13-248.65 ,medicine ,HBV ,Animals ,Humans ,Chorionic Gonadotropin, beta Subunit, Human ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Chinese hamster ovary cell ,Biological activity ,Interferon-alpha ,Molecular biology ,Half-life ,In vitro ,chemistry ,030220 oncology & carcinogenesis ,Delayed-Action Preparations ,Recombinant DNA ,Biotechnology ,medicine.drug ,HeLa Cells ,Research Article - Abstract
Background The type I human interferon (IFN) family consists of a group of cytokines with a multiplicity of biological activities, including antiviral, antitumor, and immunomodulatory effects. However, because the half-life of IFN is short, its clinical application is limited. Increasing the yield and biological activity of IFN while extending its half-life is currently the focus of IFN research. Results Two novel long-acting recombinant human IFN-α2b (rhIFN-α2b) proteins were designed in which the carboxyl-terminal peptide (CTP) of the human chorionic gonadotropin β su bunit and N-linked glycosylation sequences were linked to rhIFN-α2b. They were designated IFN-1CTPON (fused at the C-terminus of rhIFN-α2b) and IFN-2CTPON (fused at both the C-terminus and N-terminus of rhIFN-α2b). Monoclonal CHO cell strains stably and efficiently expressing the IFNs were successfully selected with methotrexate (MTX), and the highest expression levels were 1468 mg/l and 1196 mg/l for IFN-1CTPON and IFN-2CTPON, respectively. The proteins were purified with affinity chromatography and molecular sieve chromatography. IFN-1CTPON and IFN-2CTPON showed antiviral and antiproliferative activities in vitro. Notably, the half-life of IFN-1CTPON and IFN-2CTPON in vivo were three-fold and two-fold longer than that of commercially available rhIFN-α2b. Conclusions CHO cell strains stably expressing long-acting rhIFN-α2b were screened. The purified IFN-CTPON protein has biological activity and an extended half-life, and therefore potential applications.
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- 2020
4. Human bocavirus and human metapneumovirus in hospitalized children with lower respiratory tract illness in Changsha, China
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Jie-ying Zhou, Ying Peng, You-De Cao, Xiao-you Peng, Han-chun Gao, Le-Yun Xie, Zhi-ping Xie, Ya-ping Sun, Zhao-jun Duan, and Li-Li Zhong
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0301 basic medicine ,Male ,Epidemiology ,viruses ,Nasopharynx ,Prevalence ,Respiratory system ,Child ,human metapneumovirus ,Paramyxoviridae Infections ,biology ,Respiratory tract infections ,Coinfection ,Human bocavirus ,virus diseases ,Viral Load ,Hospitalization ,medicine.anatomical_structure ,Infectious Diseases ,Child, Preschool ,Original Article ,Female ,Seasons ,Viral load ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,China ,Adolescent ,030106 microbiology ,human bocavirus ,Real-Time Polymerase Chain Reaction ,Parvoviridae Infections ,03 medical and health sciences ,Age Distribution ,Human metapneumovirus ,Internal medicine ,medicine ,Bronchopneumonia ,Humans ,business.industry ,Infant, Newborn ,Public Health, Environmental and Occupational Health ,Infant ,Original Articles ,Length of Stay ,biology.organism_classification ,medicine.disease ,respiratory tract diseases ,Pneumonia ,Metapneumovirus ,business ,Respiratory tract - Abstract
BACKGROUND Lower respiratory tract illness is a major cause of morbidity and mortality in children worldwide, however, information about the epidemiological and clinical characteristics of LRTIs caused by HMPV and HBoV in China is limited. OBJECTIVES Human bocavirus (HBoV) and human metapneumovirus (HMPV) are two important viruses for children with lower respiratory tract infections (LRTI). We aimed to assay the correlation between viral load and clinical characteristics of HBoV and HMPV with LRTI in Changsha, China. METHODS Nasopharyngeal aspirates (NPAs) from children with LRTI were collected. Real-time PCR was used to screen HBoV and HMPV. Analyses were performed using SPSS 16.0 software. RESULTS Pneumonia was the most frequent diagnosis. There was no significant difference between HBoV- and HMPV-positive patients in age (P = .506) or hospitalization duration (P = .280); 24.1% and 18.2% were positive for HBoV and HMPV. HBoV infections peaked in summer (32.2%), and HMPV infections peaked in winter (28.9%). The HBoV-positive patients had a shorter hospitalization duration than the HBoV-negative patients (P = .021), and the HMPV-positive patients had a higher prevalence of fever than the HMPV-negative patients (P = .002). The HBoV viral load was significantly higher among patients aged
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- 2018
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5. Clinical characteristics and viral load of respiratory syncytial virus and human metapneumovirus in children hospitaled for acute lower respiratory tract infection
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Zhao-jun Duan, Zhi-ping Xie, Li‐jun Liu, Han-chun Gao, Xue‐mei Yang, Yi‐jie Tang, Yuning Li, Xiao‐li Yan, and Yu‐mei Li
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Male ,0301 basic medicine ,China ,medicine.medical_specialty ,Adolescent ,respiratory syncytial virus ,viruses ,030106 microbiology ,Respiratory Syncytial Virus Infections ,Real-Time Polymerase Chain Reaction ,Virus ,03 medical and health sciences ,0302 clinical medicine ,Human metapneumovirus ,Nasopharynx ,Virology ,Epidemiology ,medicine ,Humans ,030212 general & internal medicine ,Respiratory system ,Child ,Respiratory Tract Infections ,Research Articles ,Paramyxoviridae Infections ,human metapneumovirus ,biology ,Respiratory tract infections ,business.industry ,Infant, Newborn ,Infant ,virus diseases ,Viral Load ,medicine.disease ,biology.organism_classification ,Hospitalization ,Infectious Diseases ,Bronchiolitis ,Child, Preschool ,Respiratory Syncytial Virus, Human ,Immunology ,Coinfection ,Female ,epidemiology ,Metapneumovirus ,business ,Viral load ,Research Article - Abstract
Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are two common viral pathogens in acute lower respiratory tract infections (ALRTI). However, the association of viral load with clinical characteristics is not well-defined in ALRTI. To explore the correlation between viral load and clinical characteristics of RSV and HMPV in children hospitalized for ALRTI in Lanzhou, China. Three hundred and eighty-seven children hospitalized for ALRTI were enrolled. Nasopharyngeal aspirates (NPAs) were sampled from each children. Real-time PCR was used to screen RSV, HMPV, and twelve additional respiratory viruses. Bronchiolitis was the leading diagnoses both in RSV and HMPV positive patients. A significantly greater frequency of wheezing (52% vs. 33.52%, P = 0.000) was noted in RSV positive and negative patients. The RSV viral load was significant higher in children aged < 1 year (P = 0.003), children without fever and wheezing (P = 0.015 and P = 0.000), days of illness < 14 days (P = 0.002), children with bronchiolitis (P = 0.012) and children with RSV single infections (P = 0.000). No difference was found in the clinical features of HMPV positive and negative patients. The HMPV viral load had no correlation with any clinical characteristics. The incidences of severe disease were similar between single infection and coinfection for the two viruses (RSV, P = 0.221; HMPV, P = 0.764) and there has no statistical significance between severity and viral load (P = 0.166 and P = 0.721). Bronchiolitis is the most common disease caused by RSV and HMPV. High viral load or co-infection may be associated with some symptoms but neither has a significant impact on disease severity for the two viruses. J. Med. Virol. 89: 589-597, 2017. (C) 2016 Wiley Periodicals, Inc.
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- 2016
6. Identification of 12 Cases of Acute Measles Encephalitis Without Rash: Table 1
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Jie Xiong, Han-chun Gao, Zhao-jun Duan, Saizhen Zeng, Tian Yu, Bing Zhang, Yan Zhang, Le-Yun Xie, and Zhi-ping Xie
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0301 basic medicine ,Microbiology (medical) ,biology ,business.industry ,Viral encephalitis ,030106 microbiology ,Disease ,medicine.disease ,biology.organism_classification ,Virology ,Measles ,Rash ,Measles virus ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Genotype ,medicine ,030212 general & internal medicine ,Measles vaccine ,medicine.symptom ,business ,Encephalitis - Abstract
Twelve cases of acute measles encephalitis without rash were identified from October 2011 to July 2013 in Changsha city, China; 5 were found to be genotype H1 and 2 were B3. Our data suggest that screening for measles virus is necessary in children with viral encephalitis, to eliminate the disease.
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- 2016
7. Human parainfluenza virus types 1-4 in hospitalized children with acute lower respiratory infections in China
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Han Huang, Zhi-ping Xie, Zhao-jun Duan, Ni-Guang Xiao, Han-chun Gao, Sai-Zhen Zeng, Li-Li Zhong, and Bing Zhang
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0301 basic medicine ,Respiratory tract infections ,business.industry ,030106 microbiology ,medicine.disease_cause ,Virology ,Virus ,03 medical and health sciences ,Human Parainfluenza Virus ,0302 clinical medicine ,Infectious Diseases ,medicine.anatomical_structure ,Nasopharyngeal aspirate ,Genotype ,medicine ,030212 general & internal medicine ,Respiratory system ,Rhinovirus ,business ,Respiratory tract - Abstract
Human parainfluenza viruses (HPIVs) are an important cause of acute lower respiratory tract infections (ALRTIs). HPIV-4, a newly identified virus, has been associated with severe ALRTIs recently. A total of 771 nasopharyngeal aspirate samples were collected from hospitalized children between March 2010 and February 2011. HPIVs were detected by Nest-PCR, and other known respiratory viruses were detected by RT-PCR and PCR. All amplification products were sequenced. HPIVs were detected in 151 (19.58%) patients, of whom 28 (3.63%) were positive for HPIV-4, 12(1.55%) for HPIV-1, 4 (0.51%) for HPIV-2, and 107 (13.87%) for HPIV-3. Only three were found to be co-infected with different types of HPIVs. All HPIV-positive children were under 5 years of age, with the majority being less than 1 year. Only the detection rate of HPIV-3 had a significant statistical difference (χ2 = 29.648, P = 0.000) between ages. HPIV-3 and HPIV-4 were detected during the summer. Sixty (39.74%) were co-infected with other respiratory viruses, and human rhinovirus (HRV) was the most common co-infecting virus. The most frequent clinical diagnosis was bronchopneumonia, and all patients had cough; some patients who were infected with HPIV-3 and HPIV-4 had polypnea and cyanosis. No significant difference was found in clinical manifestations between those who were infected with HPIV-4 and HPIV-3. Two genotypes for HPIV-4 were prevalent, although HPIV-4a dominated. HPIV-4 is an important virus for children hospitalized with ALRTIs in China. HRV was the most common co-infecting virus. Two genotypes for HPIV-4 are prevalent, HPIV-4a dominated. J. Med. Virol. 88:2085-2091, 2016. © 2016 Wiley Periodicals, Inc.
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- 2016
8. Human adenovirus load in respiratory tract secretions are predictors for disease severity in children with human adenovirus pneumonia
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Zhao-jun Duan, Han Huang, Jieying Zhou, Bing Zhang, Zhi-ping Xie, Le-Yun Xie, Qin Liu, Sai-Zhen Zeng, Han-chun Gao, and Li-Li Zhong
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Male ,0301 basic medicine ,Serotype ,medicine.medical_specialty ,Adolescent ,Respiratory System ,030106 microbiology ,HAdV ,Biology ,Severity of Illness Index ,lcsh:Infectious and parasitic diseases ,Adenovirus Infections, Human ,03 medical and health sciences ,0302 clinical medicine ,Community-acquired pneumonia ,Virology ,Internal medicine ,Severity of illness ,medicine ,Humans ,lcsh:RC109-216 ,030212 general & internal medicine ,Risk factor ,Child ,Respiratory Tract Infections ,Children ,Respiratory tract infections ,Coinfection ,Adenoviruses, Human ,Research ,Infant ,virus diseases ,Pneumonia ,Viral Load ,medicine.disease ,eye diseases ,Community-Acquired Infections ,Infectious Diseases ,Child, Preschool ,Female ,HAdV- load ,Viral load - Abstract
Background Pneumonia is a serious public health issue and is concerned around the world. This study is to investigate the association between viral load in children with human adenovirus (HAdV) pneumonia and disease severity. Methods A total of 1313 cases of children hospitalized in Hunan Provincial People’s Hospital due to community acquired pneumonia (CAP) from April 2011 to May 2014 were enrolled in this study. Samples of nasopharyngeal aspirate were collected for the cohort. WHO criteria for CAP grading was emerged for pneumonia severity classification. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect 12 kinds of respiratory viruses. HAdV types were identified by nested PCR. The relationship between HAdV load and severity of disease was there by analyzed. Results Finally, 174 cases (174/1313, 13.3%) were positive for HAdV, and HAdV type 7 (HAdV-7) was the main serotype (76/174, 43.7%). Among the 174 cases, 70 cases were with HAdV infection alone and 104 cases were accompanied by other viruses. The patients were divided into mild pneumonia group (n = 108 cases) and severe pneumonia group (n = 66 cases). HAdV load of children in severe pneumonia group was higher than that in mild pneumonia group. Similar result was obtained in the 70 cases with HAdV infection alone after subgrouping. Relevant factors analysis results showed that severe pneumonia children presented lower onset age, more prone to fever, longer fever time, and longer hospital stay compared with that of mild pneumonia children. Children with HAdV-7 infection developed more frequently severe pneumonia. Multivariate regression analysis showed that HAdV load, age, and fever time were risk factors for pneumonia severity. Conclusion The severity of HAdV infection is significantly correlated with viral load and serotype.
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- 2018
9. Gradual replacement of all previously circulating respiratory syncytial virus A strain with the novel ON1 genotype in Lanzhou from 2010 to 2017
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Zhao-jun Duan, Wei-kai Wang, Yong-sheng Shi, Rong-fang Zhang, Donghai Liu, Zhi-ping Xie, Xuan Liang, De Chen, Yong-Jun Wang, Hui Yang, Li Guo, and Han-chun Gao
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China ,Genotype ,Observational Study ,Respiratory Syncytial Virus Infections ,Virus ,03 medical and health sciences ,0302 clinical medicine ,Gene duplication ,Humans ,Medicine ,030212 general & internal medicine ,Respiratory system ,Lanzhou ,Retrospective Studies ,Respiratory tract infections ,business.industry ,Strain (biology) ,Infant ,Outbreak ,General Medicine ,Virology ,Respiratory Syncytial Viruses ,RSV Infections ,Child, Preschool ,ON1 genotype ,030220 oncology & carcinogenesis ,respiratory syncytial virus A ,business ,Research Article - Abstract
ON1 is a novel genotype of human respiratory syncytial virus (HRSV) subtype A, in children with acute respiratory tract infections (ARTIs). However, there is not much data on the prevalence and clinical and molecular characterization in China. Our study is based on the children who had respiratory infections positive for RSV-A admitted by Gansu Provincial Maternity and Child-care Hospital in Lanzhou (northwestern China) during the last 7 epidemic seasons from 2010 to 2017. In our study, different strains of the novel RSV-A genotype ON1, first identified in Canada in December 2010, were first detected in Gansu Provincial Maternity and Child-care Hospital in August 2012 and then followed by an abrupt expansion in the number of ON1 variants in the beginning of 2014 and eventually replaced all other RSV-A strains from 2015 to 2017. ON1 is characterized by a 72-nt duplication in the C-terminal region of the highly variable attachment glycoprotein (G), predicted to lengthen the polypeptide with 24 amino acids, including a 23-aa duplication, which likely changes antigenicity. New N-glycosylation sites occurred within the 23-aa duplication and 24-aa insertion of the ON1 viruses in our study. Notably, RSV infections occurred later, but peaked sooner from the 2014/2015 to 2016/2017 epidemic seasons, compared with the previous 4 seasons. Our study concluded that genotype ON1 has caused larger outbreaks and became the predominate genotype for HRSV subgroup A in Lanzhou from 2013 to 2017, and became the sole genotype of RSV-A in 2015/2016 and 2016/2017. Our data indicate that northwest of China and the world will eventually be dominated by the ON1 RSV-A genotype, including the possibility for vaccine development. Based on trends seen in RSV-B BA genotype, which predominated for decades, there is a possibility to develop a vaccine for children in the next 10 years.
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- 2019
10. Prevalence of human metapneumovirus in children with acute lower respiratory infection in Changsha, China†
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Zhi-ping Xie, Jia Li, Rong-fang Zhang, Jing-rong Song, Li-Li Zhong, Ni-Guang Xiao, Bing Zhang, Han-chun Gao, Qiong-hua Zhou, Xiao-Fang Ding, Zhao-jun Duan, and Yun-de Hou
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Male ,China ,Adolescent ,Genotype ,viruses ,Virus ,Human metapneumovirus ,children ,Nasopharyngeal aspirate ,Virology ,Nasopharynx ,Prevalence ,Medicine ,acute lower respiratory infection ,Humans ,Child ,Respiratory Tract Infections ,Research Articles ,Phylogeny ,Molecular Epidemiology ,Paramyxoviridae Infections ,Molecular epidemiology ,biology ,Respiratory tract infections ,business.industry ,Coinfection ,Reverse Transcriptase Polymerase Chain Reaction ,Human bocavirus ,Infant, Newborn ,virus diseases ,Infant ,Sequence Analysis, DNA ,biology.organism_classification ,medicine.disease ,respiratory tract diseases ,Infectious Diseases ,reverse transcription‐polymerase chain reaction ,Child, Preschool ,Immunology ,hMPV ,RNA, Viral ,Female ,Metapneumovirus ,business ,Research Article - Abstract
Human metapneumovirus (hMPV) causes acute respiratory infections in children. The prevalence and clinical characteristics of hMPV were determined in nasopharyngeal aspirates of children in Changsha, China. Reverse transcription‐polymerase chain reaction (RT‐PCR) or PCR was employed to screen for both hMPV and other common respiratory viruses in 1,165 nasopharyngeal aspirate specimens collected from children with lower respiratory tract infections from September 2007 to August 2008. All PCR products were sequenced, and demographic and clinical data were collected from all patients. Seventy‐six of 1,165 (6.5%) specimens were positive for hMPV, of which 85.5% (65/76) occurred in the winter and spring seasons. The hMPV coinfection rate was 57.9% (44/76), and human bocavirus was the most common virus detected in conjunction with hMPV. Phylogenetic analysis revealed that 94.7% of the hMPV detected were of subgroup A2, 5.3% were subgroup B2, and none belonged to either the A1 or B1 subgroups. No significant differences were found in terms of the frequency of diagnosis and clinical signs between either the co‐ and mono‐infection groups, or between patients with and without underlying diseases. It was concluded that hMPV is an important viral pathogen in pediatric patients with lower respiratory tract infections in Changsha. Only hMPV genotypes A2 and B2 were co‐circulating in this locality; human bocavirus was the most common coinfecting virus, and coinfection did not affect disease severity. J. Med. Virol. 85:546–553, 2013. © 2013 Wiley Periodicals, Inc.
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- 2013
11. Epidemiology of human adenovirus infection in children hospitalized with lower respiratory tract infections in Hunan, China
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Zhi-ping Xie, Xin Zhao, Ni-Guang Xiao, Fei Zhang, Han-chun Gao, Bing Zhang, Li-Li Zhong, Zhao-jun Duan, Le-Yun Xie, and Qin Liu
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Male ,polymerase chain reaction ,law.invention ,Adenovirus Infections, Human ,0302 clinical medicine ,law ,Nasopharynx ,Genotype ,Epidemiology ,Prevalence ,Medicine ,030212 general & internal medicine ,Child ,Respiratory Tract Infections ,Research Articles ,Respiratory tract infections ,Coinfection ,Incidence (epidemiology) ,Incidence ,virus diseases ,adenovirus ,Intensive care unit ,Hospitalization ,Infectious Diseases ,Child, Preschool ,Acute Disease ,030211 gastroenterology & hepatology ,Female ,Research Article ,medicine.medical_specialty ,China ,Adolescent ,03 medical and health sciences ,acute lower respiratory tract infection ,children ,Virology ,Internal medicine ,Humans ,Adenovirus infection ,business.industry ,Adenoviruses, Human ,Infant, Newborn ,Infant ,Pneumonia ,medicine.disease ,eye diseases ,business - Abstract
To investigate the current genotypes of circulating human adenovirus (HAdV) strains, we molecularly genotyped HAdV in the nasopharyngeal aspirates (NPAs) of patients with acute lower respiratory tract infections (ALRTIs) and attempted to determine their associations with clinical symptoms. A total of 4751 NPA samples were collected from 4751 patients admitted to Hunan Provincial People's Hospital from September 2007 to March 2014, of which 447 (9.4%) samples were HAdV positive. Fourteen different HAdV types were identified; HAdV types 1 to 7 (HAdV 1‐7) were identified in 95.7% of the 447 NPA samples with HAdV‐7 and HAdV‐3 being the most prevalent. In addition, 93.3% (417 of 447) of patients were younger than 5 years. The incidence of HAdV infection peaked in summer. Different HAdV types showed a predilection for different age groups and different seasonal distribution patterns. Coinfection of HAdVs and other respiratory viruses was detected in 63.3% (283 of 447) of the HAdV‐positive samples. The most common clinical diagnosis was pneumonia and the most common symptoms were fever and cough. In comparison with children infected with HAdV‐3 alone, those infected with HAdV‐7 alone had an increased frequency of severe pneumonia involvement (11.6% vs 32.4%; P = 0.031), higher intensive care unit admission rates (7.0% vs 26.5%; P = 0.019), and a longer length of hospital stay (P = 0.03). Mixed infections in younger children were associated with a longer hospital stay (P = 0.023). Our results demonstrate the recent changes in the trends of circulating HAdV genotypes associated with ALRTIs in Hunan China.
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- 2016
12. Identification of 12 Cases of Acute Measles Encephalitis Without Rash
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Sai-Zhen, Zeng, Bing, Zhang, Yan, Zhang, Le-Yun, Xie, Jie, Xiong, Tian, Yu, Zhi-Ping, Xie, Han-Chun, Gao, and Zhao-Jun, Duan
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Male ,China ,Child, Preschool ,Acute Disease ,Measles Vaccine ,Humans ,Infant ,Female ,Exanthema ,Child ,Measles - Abstract
Twelve cases of acute measles encephalitis without rash were identified from October 2011 to July 2013 in Changsha city, China; 5 were found to be genotype H1 and 2 were B3. Our data suggest that screening for measles virus is necessary in children with viral encephalitis, to eliminate the disease.
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- 2016
13. A novel hepatovirus identified in wild woodchuck Marmota himalayana
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Zhi-ping Xie, Han-chun Gao, Lili Pang, Cui-yuan Zhang, Jie-mei Yu, Lili Li, Xiaoman Sun, Zhao-jun Duan, W. Ian Lipkin, Shan Lu, Jian-guo Xu, Yuanyun Ao, and Guang-Cheng Xie
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0301 basic medicine ,Most recent common ancestor ,Genotype ,Picornavirus ,viruses ,Genome, Viral ,Article ,Virus ,Evolution, Molecular ,Epitopes ,Open Reading Frames ,03 medical and health sciences ,Animals ,Hepatovirus ,Codon ,Antigens, Viral ,Phylogeny ,Tropism ,Genetics ,Base Composition ,Multidisciplinary ,biology ,Phylogenetic tree ,fungi ,virus diseases ,Bayes Theorem ,Genomics ,biochemical phenomena, metabolism, and nutrition ,Marmota himalayana ,biology.organism_classification ,Virology ,digestive system diseases ,030104 developmental biology ,Marmota ,Codon usage bias ,Nucleic Acid Conformation ,RNA, Viral - Abstract
Hepatitis A virus (HAV) is a hepatotropic picornavirus that causes acute liver disease worldwide. Here, we report on the identification of a novel hepatovirus tentatively named Marmota Himalayana hepatovirus (MHHAV) in wild woodchucks (Marmota Himalayana) in China. The genomic and molecular characterization of MHHAV indicated that it is most closely related genetically to HAV. MHHAV has wide tissue distribution but shows tropism for the liver. The virus is morphologically and structurally similar to HAV. The pattern of its codon usage bias is also consistent with that of HAV. Phylogenetic analysis indicated that MHHAV groups with known HAVs but forms an independent branch and represents a new species in the genus Hepatovirus within the family Picornaviridae. Antigenic site analysis suggested MHHAV has a new antigenic property to other HAVs. Further evolutionary analysis of MHHAV and primate HAVs led to a most recent common ancestor estimate of 1,000 years ago, while the common ancestor of all HAV-related viruses including phopivirus can be traced back to 1800 years ago. The discovery of MHHAV may provide new insights into the origin and evolution of HAV and a model system with which to explore the pathogenesis of HAV infection.
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- 2016
14. Human Respiratory Syncytial Virus in Children with Acute Respiratory Tract Infections in China
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Xin-hui Yuan, Yun-de Hou, Yu Jin, Rong-fang Zhang, Ming-wei Guo, Qiong-hua Zhou, Han-chun Gao, Na Liu, Ni-Guang Xiao, Zhi-ping Xie, Jing-rong Song, Kun-Long Yan, and Zhao-jun Duan
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Male ,Microbiology (medical) ,China ,Adolescent ,Genotype ,Paramyxoviridae ,Molecular Sequence Data ,Sequence Homology ,Respiratory Syncytial Virus Infections ,Virology ,Nasopharynx ,Prevalence ,medicine ,Cluster Analysis ,Humans ,Child ,Mononegavirales ,Respiratory Tract Infections ,Phylogeny ,Respiratory tract infections ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Respiratory disease ,Infant, Newborn ,Infant ,Sequence Analysis, DNA ,Pneumovirus ,medicine.disease ,biology.organism_classification ,medicine.anatomical_structure ,Child, Preschool ,Respiratory Syncytial Virus, Human ,Immunology ,Coinfection ,RNA, Viral ,Respiratory virus ,Female ,Respiratory tract - Abstract
There are limited data on the prevalence and clinical and molecular characterization of human respiratory syncytial virus (HRSV) in children with acute respiratory tract infections (ARTIs) in China. From December 2006 to March 2009, 894 nasopharyngeal aspirates (NPAs) were collected from children under 14 years of age with ARTIs. Samples were screened for HRSV and genotyped by reverse transcription-PCR (RT-PCR) and sequencing. Demographic and clinical information was recorded. A total of 38.14% (341/894) of samples were positive for HRSV. Phylogenetic analysis revealed that 60.4% of the selected 227 RSV strains were GA2, 34.4% were BA, 4.8% were GB2, and 0.4% were GB3. A total of 40.47% of all of the RSV-positive samples were coinfected with other respiratory viruses, and adenovirus was the most common additional respiratory virus. No statistical differences were found in the frequency of diagnosis and symptoms between the coinfection group and monoinfection group. Additionally, no statistical differences were found in epidemiological characterizations or disease severity between genotype BA- and GA2-positive patients, except for a greater frequency of lower respiratory tract infections (LRTIs) (mostly bronchitis)with BA. HRSV is the most important viral pathogen in Chinese children with ARTIs. Four genotypes (i.e., GA2, BA, GB2, and GB3) circulate locally, and the predominant genotype may shift between seasons. Coinfection with other viruses does not affect disease severity. HRSV genotypes were not associated with different epidemiological characterizations or disease severity.
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- 2010
15. Prevalence and clinical characteristics of human CoV-HKU1 in children with acute respiratory tract infections in China
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Zi-qian Xu, Jingrong Song, Yang Zhao, Zhi-ping Xie, Ni-Guang Xiao, Yun-de Hou, Xin-hui Yuan, Kun-long Yan, Han-chun Gao, Yu Jin, and Zhao-jun Duan
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Male ,medicine.medical_specialty ,China ,Acute respiratory tract infection ,Adolescent ,Coronaviridae ,Coronaviridae Infections ,Molecular Sequence Data ,Prevalence ,Comorbidity ,Article ,HCoV-HKU1 ,stomatognathic system ,Nasopharyngeal aspirate ,Virology ,Internal medicine ,Nasopharynx ,Epidemiology ,Genotype ,medicine ,Cluster Analysis ,Humans ,Child ,Children ,Respiratory Tract Infections ,Phylogeny ,Aged ,Polymorphism, Genetic ,Respiratory tract infections ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Respiratory disease ,Infant, Newborn ,virus diseases ,Infant ,Sequence Analysis, DNA ,medicine.disease ,Pneumonia ,Infectious Diseases ,Child, Preschool ,Immunology ,Reverse transcription polymerase chain reaction ,RNA, Viral ,Female ,Seasons ,business - Abstract
Background: Human CoV-HKU1 (HCoV-HKU1) has been isolated from a 71-year-old man with pneumonia; however, the impact and role of emerging HCoV-HKU1 have not been defined in children with acute respiratory tract infection (ARTI). Objective: To investigate the Prevalence and clinical characteristics of HCoV-HKU1 in children with ARTI in Lanzhou, China. Study design: The reverse transcription polymerase chain reaction (RT-PCR) or PCR was employed to screen HCoV-HKU1 and other common respiratory viruses in 645 nasopharyngeal aspirate (NPA) specimens collected from children with ARTI from November 2006 to October 2008. All PCR positive products were sequenced. And the demographic and clinical data were collected for all patients. Results: Nineteen of 645 (2.95%) specimens tested positive for HCoV-HKU1, and all HCoV-HKU1 positive specimens were distributed in the winter and spring season. The HCoV-HKU1 co-infection rate with other respiratory viruses was 47.37% (9/19). There was no statistically significant difference in the detection rate between groups by age or gender, except between patients with and without underlying diseases. The phylogenetic analysis indicated that HCoV-HKU1 genotype B was circulating in the years 2007 and 2008 in children with ARTI in Lanzhou, China. Conclusions: HCoV-HKU1 is an uncommon virus existing among Chinese children with ARTI. Children with underlying diseases are more vulnerable to viral infection. Only HCoV-HKU1 genotype B circulated locally.
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- 2010
16. Novel Human Bocavirus in Children with Acute Respiratory Tract Infection
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Yang Zhao, Yu Jin, Yun-De Hou, Jing-rong Song, Zi-qian Xu, Han-Chun Gao, Zhi-Ping Xie, Wei-xia Chen, Ni-Guang Xiao, Zhao-jun Duan, and Kun-long Yan
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Microbiology (medical) ,Male ,medicine.medical_specialty ,China ,Epidemiology ,Parvoviridae Infections ,lcsh:Medicine ,Respiratory Syncytial Virus Infections ,Polymerase Chain Reaction ,lcsh:Infectious and parasitic diseases ,respiratory infections ,children ,Human bocavirus ,Medicine ,Humans ,lcsh:RC109-216 ,viruses ,Respiratory system ,Acute respiratory tract infection ,Respiratory Tract Infections ,Phylogeny ,Respiratory tract infections ,biology ,business.industry ,Incidence ,lcsh:R ,Dispatch ,Infant ,acute respiratory tract infection ,biology.organism_classification ,Virology ,respiratory tract diseases ,Infectious Diseases ,Child, Preschool ,Immunology ,Female ,Seasons ,business - Abstract
Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.
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- 2010
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17. Prevalence and Clinical and Molecular Characterization of Human Metapneumovirus in Children With Acute Respiratory Infection in China
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Zhao-jun Duan, Jing-rong Song, Yunde Hou, Zhi-Ping Xie, Bing Zhang, Han-Chun Gao, Ni-Guang Xiao, Rong-fang Zhang, and Xin-hui Yuan
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Male ,Microbiology (medical) ,China ,Genotype ,viruses ,Prevalence ,Sequence Homology ,Comorbidity ,Viral Proteins ,stomatognathic system ,Human metapneumovirus ,Nasopharynx ,medicine ,Cluster Analysis ,Humans ,Child ,Respiratory Tract Infections ,Phylogeny ,Molecular Epidemiology ,Paramyxoviridae Infections ,Respiratory tract infections ,biology ,Molecular epidemiology ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Incidence ,Incidence (epidemiology) ,Respiratory disease ,Infant, Newborn ,Infant ,virus diseases ,Respiratory infection ,Sequence Analysis, DNA ,medicine.disease ,biology.organism_classification ,Virology ,respiratory tract diseases ,Infectious Diseases ,medicine.anatomical_structure ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Immunology ,Female ,Metapneumovirus ,Seasons ,business ,Respiratory tract - Abstract
Human metapneumovirus (HMPV), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (ARTIs). However, the prevalence and molecular characteristics of HMPV in China are still unclear.A total of 661 nasopharyngeal aspirates (NPA) specimens were collected from 661 children with ARTIs between December 2006 and November 2008. Specimens were screened for HMPV by reverse transcription-polymerase reaction. All positive amplification products were confirmed by sequencing.HMPV was detected in 45 patients (6.80%) of the 661 children. The HMPV-infected patients were from 29 days to 9 years of age. A high incidence of HMPV infection (84.4%) was observed during the winter-spring season. Of the 45 HMPV-positive patients, 25 (55.6%) were co-infected with other respiratory viruses, and respiratory syncytial virus (RSV) was the most common additional respiratory virus. The most common clinical diagnosis was bronchopneumonia (57.8%) and cough (88.9%) was the most common clinical symptom. Phylogenetic analysis of the F gene revealed that 80% of the HMPV detected were A2, 2.2% were A1, and 17.8% were B1. Statistical analyses showed that sex, ages, seasons, and severity of the disease did not correlate with HMPV genotype (P = 0.986, 0.347, 0.660, 0.252), but viral coinfection with HMPV increased hospitalization rates (P = 0.005).HMPV was frequently detected in the pediatric patients with ARTI in China. RSV was the most common coinfection virus and coinfection increased hospitalization rates. All HMPV subgroups except B2 cocirculated and there was no association found between HMPV genotypes and severity of disease.
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- 2010
18. Prevalence and Clinical Characterization of a Newly Identified Human Rhinovirus C Species in Children with Acute Respiratory Tract Infections
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Zhao-jun Duan, Zhi-ping Xie, Rong-fang Zhang, Han-chun Gao, Xin-hui Yuan, Li-shu Zheng, Yu Jin, Zi-qian Xu, Yun-de Hou, and Jing-rong Song
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Male ,Microbiology (medical) ,China ,Adolescent ,Rhinovirus ,Molecular Sequence Data ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Virus ,stomatognathic system ,Nasopharynx ,Virology ,Genotype ,Bronchopneumonia ,Prevalence ,otorhinolaryngologic diseases ,medicine ,Humans ,Child ,Respiratory Tract Infections ,Phylogeny ,Nose ,Viral Structural Proteins ,Picornaviridae Infections ,Sequence Homology, Amino Acid ,Respiratory tract infections ,Reverse Transcriptase Polymerase Chain Reaction ,Respiratory disease ,Infant, Newborn ,Infant ,virus diseases ,Sequence Analysis, DNA ,medicine.disease ,medicine.anatomical_structure ,Bronchiolitis ,Child, Preschool ,Female ,Seasons ,circulatory and respiratory physiology ,Respiratory tract - Abstract
Human rhinovirus C (HRV-C) is a newly identified genotype of HRV found in patients with respiratory tract infections (RTIs); however, its epidemiological profile and clinical characteristics are not well understood. In this study, Chinese children with RTIs were screened for HRV-C and their epidemiological and clinical characteristics were analyzed. From December 2006 to November 2007, 406 nasopharyngeal aspirates from children younger than 14 years of age with RTIs were screened for HRV and other common respiratory viruses by PCR or reverse transcription-PCR. Two-hundred twenty-four (55.2%) of the specimens were infected with at least one virus, including 53 patients with HRV (13%). HRV-A, HRV-B, and HRV-C were detected in 22, 12, and 19 specimens, respectively. HRV-C was detected mainly from December 2006 to April 2007 and from October to November 2007, with peaks in December and April (10/19). Acute upper respiratory infection and bronchopneumonia were observed in 53 and 37% of the cases, respectively. The most common symptoms were cough (82%), runny nose (53%), and fever (37%). Wheezing and bronchiolitis were less common in patients infected with HRV-C than in those infected with respiratory syncytial virus (RSV). Partial sequencing of the genes coding for VP4 and VP2 revealed that the HRV-C strains were 56 to 62% identical at the amino acid level to HRV-B and HRV-A reference strains and 80 to 99% identical to HRV-C reference strains. In conclusion, HRV-C is an important cause of RTIs in children, and highly diversified strains of HRV-C are prevalent in China. HRV-C may produce different epidemiological features, and patients infected with HRV-C may exhibit different clinical features from patients infected with RSV or HRV-A/B.
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- 2009
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19. Purification of recombinant human interferon-ε and oligonucleotide microarray analysis of interferon-ε-regulated genes
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Zhi-ping Xie, Wu-ping Li, Zhao-jun Duan, Han-chun Gao, Yun-De Hou, Li-shu Zheng, Hui Zhang, and Fu-wang Peng
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Cytotoxicity, Immunologic ,DNA, Complementary ,Gene Expression ,In Vitro Techniques ,Interferon alpha-2 ,medicine.disease_cause ,Antiviral Agents ,law.invention ,Interferon ,law ,Complementary DNA ,Gene expression ,Escherichia coli ,medicine ,Humans ,Gene ,Cell Proliferation ,Oligonucleotide Array Sequence Analysis ,Base Sequence ,biology ,Brain ,Interferon-alpha ,biology.organism_classification ,Molecular biology ,Recombinant Proteins ,Killer Cells, Natural ,Gene expression profiling ,Vesicular stomatitis virus ,Interferon Type I ,Recombinant DNA ,HeLa Cells ,Biotechnology ,medicine.drug - Abstract
Recently identified interferon-epsilon (IFN-epsilon) belongs to type I interferons. IFN-epsilon is highly and constitutively expressed in the brain, but its biochemical and biological characteristics are poorly understood. In this study, full-length IFN-epsilon cDNA was cloned from human peripheral blood lymphocyte by RT-PCR, and was expressed in Escherichia coli (E. coli). Reverse phase high pressure liquid chromatography was used to purify recombinant human IFN-epsilon (rhIFN-epsilon) and to facilitate refolding of the protein. About 0.8mg of highly purified rhIFN-epsilon protein was obtained from 100ml of E. coli culture. Functional study of rhIFN-epsilon demonstrated that the antiviral activity of rhIFN-epsilon was 6+/-0.5x10(5)IU/mg, which was lower than that of rhIFN-alpha-2b in the WISH-VSV (WISH cells infected with vesicular stomatitis virus) assay system. As for the activity to promote NK cytotoxicity and antiproliferation activities, rhIFN-epsilon was about 60 times less potent than rhIFN-alpha-2b. However, oligonucleotide microarray analyses revealed dramatic differences in gene expression profiles of cultured human cells treated with IFN-epsilon and IFN-alpha-2b. Particularly, differential regulation of genes related to central nervous system by rhIFN-epsilon suggests a role for IFN-epsilon in maintenance of the structure and function of brain.
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- 2007
20. Outbreak of febrile illness caused by coxsackievirus A4 in a nursery school in Beijing, China
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Zhao-jun Duan, Zhi-ping Xie, Jinsong Li, Han-chun Gao, Xiao-Gen Dong, Jie Li, Xiao-Xin Yang, Dandi Li, Meng Qin, and Jun-Yong Yang
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Serotype ,Male ,Fever ,Molecular Sequence Data ,Short Report ,Coxsackievirus Infections ,Disease ,Biology ,Coxsackievirus ,Disease cluster ,medicine.disease_cause ,Schools, Nursery ,Disease Outbreaks ,Beijing ,Virology ,Control ,medicine ,Infection control ,Humans ,Preschool ,Child ,Enterovirus ,Infection Control ,Prevention ,Outbreak ,Infant ,Sequence Analysis, DNA ,biology.organism_classification ,Infectious Diseases ,Child, Preschool ,RNA, Viral ,Female ,Coxsackievirus A4 - Abstract
Background Coxsackievirus A4 (CV-A4) is classified as human enterovirus A according to its serotype. CV-A4, an etiological agent of hand, foot, and mouth disease, affects children worldwide and can circulate in closed environments such as schools and hospitals for long periods. Findings An outbreak of febrile illness at a nursery school in Beijing, China, was confirmed to be caused by CV-A4. Phylogenetic analysis of the complete genome of the isolated strain showed that the virus belongs to the same cluster as the predominant CV-A4 strain in China. This outbreak was controlled by effective measures. Conclusions The early identification of the pathogen and timely intervention may be the most critical factors in controlling an outbreak caused by CV-A4 in a preschool.
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- 2015
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21. Immunogenicity of recombinant human bocavirus-1,2 VP2 gene virus-like particles in mice
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Zhong-Hua Deng, Zhi-Ping Xie, You-De Cao, Bing Zhang, Ye-xia Hao, Zhao-Jun Duan, Le-Yun Xie, Li-Li Zhong, Han-Chun Gao, and Li-Hong Yao
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Male ,Enzyme-Linked Immunospot Assay ,Time Factors ,Injections, Intradermal ,viruses ,Immunology ,chemical and pharmacologic phenomena ,Enzyme-Linked Immunosorbent Assay ,Biology ,Cross Reactions ,Antibodies, Viral ,complex mixtures ,Injections, Intramuscular ,Immunoglobulin G ,Mice ,Immune system ,Virus-like particle ,Adjuvants, Immunologic ,Human bocavirus ,Immunology and Allergy ,Animals ,Avidity ,Alum adjuvant ,Research Articles ,Immunization Schedule ,Immunity, Cellular ,Mice, Inbred BALB C ,Immunogenicity ,Virion ,Respiratory infection ,Viral Vaccines ,T-Lymphocytes, Helper-Inducer ,biochemical phenomena, metabolism, and nutrition ,Virology ,Immunity, Humoral ,biology.protein ,Alum Compounds ,Capsid Proteins ,Antibody - Abstract
Human bocavirus (HBoV), a recently identified pathogen with a worldwide distribution is closely related to paediatric acute respiratory infection and gastroenteritis. The present study was performed to evaluate the immunogenicity of HBoV1 and HBoV2 virus-like particles (VLPs) as vaccine candidates in mice. Both HBoV1 and HBoV2 VLPs were expressed in the bacmid virus–SF9 cell system. Mice were inoculated three times at 3-week intervals with HBoV VLPs at one dose intramuscular (i.m.) or intradermal (i.d.) with or without the addition of the alum adjuvant. ELISA was used to detected antibody, and ELISPOT was used to test cellular immune responses. HBoV-specific IgG antibodies were induced and alum adjuvant improved the antibody titres and avidity, while the inoculation pathway had no influence. T helper type 1/ type 2 immune responses were balanced induced by HBoV1 VLPs but not HBoV2 VLPs. Serum IgG antibody cross-reactivity rates of the two subtypes were similar, but cross-reactions of HBoV1 immunization groups were higher. The single i.m. group had more interferon-γ-secreting splenocytes. These data indicate that HBoV VP2 VLPs have good immunogenicity with induction of strong humoral and cellular immune responses, and they may be potential candidate vaccines for HBoV infection.
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- 2014
22. [Establishment and clinical application of TapMan real-time RT-PCR method for detection of HHV-6]
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Qian-Qian, Chen, Bing, Zhang, Zhi-Ping, Xie, Jin-Song, Li, Han-Chun, Gao, Ni-Guang, Xiao, Le-Yun, Xie, Tian, Yu, Sai-Zhen, Zeng, Ping, Gong, and Zhao-Jun, Duan
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Reverse Transcriptase Polymerase Chain Reaction ,Herpesvirus 6, Human ,Humans ,Reproducibility of Results ,Real-Time Polymerase Chain Reaction - Abstract
To establish a rapid, sensitive and specific real-time PCR method for detection of Human Herpesvirus-6 (HHV-6).According to the reference, a pair of primers and a probe were designed located in U65-66 gene and to set up the standards. We established a real-time RT-PCR method for detection of HHV-6, and to verify the specificity, sensitivity, reproducibility.The correlation coefficient was 0.999, E = 97.9%, the coefficient of variation values of Ct were 0.61% and 3.13% in real-time PCR assay for inter and intra assay, respectively. The results of all viruses were negative except of HHV-6 for the assay. The quantitative detection limit of the assay was 3 x 10(0) copies/microl.The real-time PCR assay is highly specific, sensitive and reproducible, which can be used to quatitative detecting clinical samples.
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- 2013
23. [Virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia]
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Le-Yun, Xie, Li-Li, Zhong, Bing, Zhang, Zhao-Jun, Duan, Zhi-Ping, Xie, Han-Chun, Gao, Qian-Qian, Chen, Zhong-Hua, Deng, Lin, Lin, Xiao-Qian, Gao, and Ni-Guang, Xiao
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Male ,Adolescent ,Reverse Transcriptase Polymerase Chain Reaction ,Adenoviruses, Human ,Child, Preschool ,Viruses ,Humans ,Infant ,Female ,Pneumonia ,Seasons ,Child ,Bronchoalveolar Lavage Fluid - Abstract
To investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.Bronchoalveolar lavage fluid [BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011. Nested- or reverse transcription Polymerase chain reaction (PCR or RT-PCR) was used to screen Adenovirus (ADV), Human Bocavirus (HBoV), Parainfluenzaviruses1-4 (PIV1-4), Human Respiratory Syneytial virus (RSV), Influenza virus A (IFVA), Influenza virus B (IFVB), Human Rhinovirus(HRV), Human Metapneumovirus (HMPV), human coronaviruses NL63 and HKU1 (HCoV-NL63, HCoV- HKU1).Among the 122 bronchoalveolar lavage fluid, viral agents were detected in 60 samples(49.1%), among which ADV (40.98%) was the most common virus, followed by RSV (7.37%) and HBoV (7.37%). Two viruses were detected in 21 individual (35%) samples, of which 20 were dual positive for ADV (40%).ADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year. And its Coinfection with other respiratory viruses was common.
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- 2013
24. Prevalence of adenovirus in children with acute respiratory tract infection in Lanzhou, China
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Kun-long Yan, Rong-fang Zhang, Xin-hui Yuan, Yun-de Hou, Jing-rong Song, Zhi-ping Xie, Han-chun Gao, Zhao-jun Duan, and Yu Jin
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Male ,Serotype ,China ,Adolescent ,Respiratory tract infection ,Adenoviridae Infections ,Polymerase Chain Reaction ,Nasopharynx ,Virology ,Genotype ,Prevalence ,medicine ,Humans ,Adenovirus ,Serotyping ,Child ,Respiratory Tract Infections ,Children ,Acute respiratory tract infection ,Respiratory tract infections ,business.industry ,Adenoviruses, Human ,Research ,Incidence (epidemiology) ,Infant, Newborn ,Infant ,virus diseases ,Respiratory infection ,eye diseases ,PCR ,Infectious Diseases ,medicine.anatomical_structure ,Child, Preschool ,Female ,Crackles ,medicine.symptom ,business ,Respiratory tract - Abstract
Background Human adenovirus (HAdV) is an important agent causing respiratory tract infection in children. Information on the epidemiological and clinical features of HAdV is limited in children with acute respiratory tract infections (ARTIs) in China, especially those of a novel genotype, Ad55. Methods In total, 1169 nasopharyngeal aspirates were collected from children younger than 14 years with ARTIs between November 2006 and November 2009. The polymerase chain reaction (PCR) was used to screen HAdVs. All PCR-positive products were sequenced. Results 74 of 1169 (6.33%) specimens were positive for HAdVs. Among positive cases, AdV3 (58/74) was detected most frequently, followed by AdV11 (10/74), AdV2 (2/74), AdV7 (2/69), AdV6 (1/74), and AdV1 (1/74). AdV55 was found in one case. The incidence of HAdV infection peaked in children aged 3–7 years. The most common clinical diagnosis was upper respiratory infection, and the most common syndrome was fever and cough.The comparison of HAdV and RSV group revealed that Children infected with group AdV were significant older than children infected with group RSV, had more fever but less frequently wheezing, and cough, crackles, and cyanosis, The duration of hospitalization between the AdV group and RSV group was not significant, but a greater frequency of LRTIs was observed in RSV group. Conclusions HAdV is an important viral agent in children with ARTIs in Lanzhou City, China. Multiple HAdV serotypes co-circulated with Ad3, which was predominant in this 3-year study. The novel AdV55 genotype was found in one case. No fixed seasonal rhythm could be identified.
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- 2013
25. [The detection and clinical feature of HcoV-nL63 in children with acute respiratory tract infection in Lanzhou city]
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Yu, Jin, Rong-Fang, Zhang, Zhi-Ping, Xie, Han-Chun, Gao, Kun-Long, Yan, Xin-Hui, Yuan, Jing-Rong, Song, Yun-De, Hou, and Zhao-Jun, Duan
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Male ,China ,Coronavirus NL63, Human ,Adolescent ,Child, Preschool ,Acute Disease ,Infant, Newborn ,Prevalence ,Humans ,Infant ,Female ,Child ,Respiratory Tract Infections - Abstract
To investigate the prevalence and clinical characterization of HCoV-NL63 (NL63) in children with acute respiratory tract infections (ARTIs) in Lanzhou with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously city,China.From November 2006 to October 2009,1169 nasopharyngeal aspirates (NPA) were collected from children under 14 years old with ARTIs. Samples were screened for NL63 using a reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Demography and clinical information were recorded.NL63 was detected in 35 (2.99%) of the 1169 children. The peak of the positive rate were in August, September 2007, July, August 2008 (23.53%,17.65%, 50%, 33.33% separately). There are no NL63 positive samples was detected in December, 2007 to February 2009. 25 (25/35, 71.43%) were co-infected with other respiratory viruses, and human rhinovirus (HRV) were the most common additional respiratory virus. No significant differences of infective rate of NL63 was found betweenor = 3 years age group and3 years age group. Bronchiolitis and pneumonia were the most frequent diagnoses in NL63 positive patients and the major symptoms were fever and cough in our study. Between the monoinfection group and the coinfection group of NL63-positive patients, no differences were found in symptoms and clinical diagnoses except symptoms of gastrointestinal.HCoV-NL63 is an important pathogen of acute respiratory tract infection in children in Lanzhou city. The peak of HCoV-NL63 infections was in summer. There were annual differences in the prevalence of HCoV-NL63. HCoV-NL63 infections existed a high rate of mixed infection, and mixed infection does not increase the severity of the disease.
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- 2013
26. [The epidemiological study of adenovirus in children with respiratory tract infections in Nanjing area from 2010 to 2011]
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Xiao-Qian, Gao, Yu, Jin, Zhi-Ping, Xie, Han-Chun, Gao, Le-Yun, Xie, Jian, Zhang, and Zhao-Jun, Duan
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Male ,China ,Adenoviridae Infections ,Child, Preschool ,Molecular Sequence Data ,Humans ,Infant ,Female ,Child ,Respiratory Tract Infections ,Phylogeny ,Adenoviridae - Abstract
To investigate the epidemiological features and types of human adenoviruses (ADV) in children with acute respiratory tract infection in Nanjing area, China. Nasopharyngeal aspirates and nasopharyngeal swabs were collected from 644 outpatients or hospitalized pediatric patients with ARTI at the Children Hospital of Nanjing, Jiangsu Province, China, between August 2010 and July 2011. Adenoviruses were identified and typed from the collected clinical specimens by nested-PCR based on the partial region of the hexon gene. Other 12 respiratory viruses including human bocavirus (HBoV), respiratory syncytial virus (RSV), human rhinovirus (HRV), parainfluenza viruses 1-4 (PIV1-4), influenza virus A/B (IFVA/B), human metapneumovirus (HMPV), human coronavirus NL63 and HKU1 (HCoV-HKU1 and HCoV-NL63) were also identified by PCR method. All PCR positive products were sequenced and phylogenetic analysis was conducted. It was showed that adenoviruses were detected in 171 patients out of 644 (26. 55%) children, 120 (70.18%, 120/171) for ADV3, 16 (9.36%,16/171) for ADV7, 12 (7.02%, 12/171) for ADV1, 10 (5.85%, 10/171) for ADV2, 6 (3.51%, 6/171) for ADV5, 3 (1.75%, 3/171) for ADV6, 3 (1.75%, 3/171) for ADV57, and 1 (0.58%,1/171) for ADV41. ADV infection could occur in any season. There was a higher possibility of ADV infection from April to July in 2011. Most cases (96.49%) were younger than 7 years old. A total of 99 of the 171 ADV-positive children (57.89%) were co-infected with other respiratory viruses. Respiratory syncytial virus (RSV) and human rhinovirus (HRV) were the most common additional respiratory viruses, Lower respiratory tract infections were the most frequent diagnoses made in the hospital, in which there were 52 pneumonia (30.4%) cases.ADV is one of the most important pathogens of acute respiratory tract infection in children in Nanjing area, and adenovirus type 3 was the most prevalent serotype. It is important to develop long-term surveillance.
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- 2012
27. [Prevalence and clinical characteristics of coronavirus NL63 infection in children hospitalized for acute lower respiratory tract infections in Changsha]
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Fei, Zhang, Bing, Zhang, Zhi-Ping, Xie, Han-Chun, Gao, Xin, Zhao, Li-Li, Zhong, Qiong-Hua, Zhou, Yun-De, Hou, and Zhao-Jun, Duan
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Hospitalization ,Male ,China ,Child, Preschool ,Acute Disease ,Prevalence ,Humans ,Infant ,Female ,Coronavirus Infections ,Respiratory Tract Infections - Abstract
The main objective of this study was to explore the prevalence and clinical characteristics of human coronavirus NL63 infection in hospitalized children with acute lower respiratory tract infection (ALRTI) in Changsha.Nasopharyngeal aspirates (NPA) samples were collected from 1185 hospitalized children with ALRTI at the People's Hospital of Hunan province, between September 2008 and October 2010. Reverse transcriptase polymerase chain reaction (RT-PCR) was employed to screen for coronavirus NL63, which is a 255 bp fragment of a part of N gene. All positive amplification products were confirmed by sequencing and compared with those in GenBank.The overall frequency of coronavirus NL63 infection was 0.8%, 6 (60%) out of the coronavirus NL63 positive patients were detected in summer, 2 in autumn, 1 in spring and winter, respectively. The patients were from 2 months to two and a half years old. The clinical diagnosis was bronchopneumonia (60%), bronchiolitis (30%), and acute laryngotracheal bronchitis (10%). Four of the 10 cases had critical illness, 4 cases had underlying diseases, and 7 cases had mixed infection with other viruses. The homogeneity of coronavirus NL63 with those published in the GenBank at nucleotide levels was 97%-100%.Coronavirus NL63 infection exists in hospitalized children with acute lower respiratory tract infection in Changsha. Coronavirus NL63 infections are common in children under 3 years of age. There is significant difference in the infection rate between the boys and the girls: the boys had higher rate than the girls. The peak of prevalence of the coronavirus NL63 was in summer. A single genetic lineage of coronavirus NL63 was revealed in human subjects in Changsha. Coronavirus NL63 may also be one of the lower respiratory pathogen in China.
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- 2012
28. [Molecular epidemiological and clinical feature of human metapneumovirus in children with acute lower respiratory tract infection in Changsha area from 2007 to 2011]
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Xin, Zhao, Bing, Zhang, Zhao-Jun, Duan, Zhi-Ping, Xie, Han-Chun, Gao, Ni-Guang, Xiao, Li-Li, Zhong, Qiong-Hua, Zhou, Fei, Zhang, Jing, Zhang, and Chang-Qing, Cao
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Male ,China ,Molecular Epidemiology ,Paramyxoviridae Infections ,Time Factors ,Acute Disease ,Humans ,Infant ,Female ,Metapneumovirus ,Respiratory Tract Infections - Abstract
To understand the epidemiological characteristics of human metapneumovirus (hMPV) infection in children with acute lower respiratory tract infection (ALRTI) in Changsha area, China.A total of 2613 nasopharyngeal aspirate (NPA) specimens were collected from hospitalized children with ARTIs between September 2007 and February 2011 (a period of 3.5 years) in Changsha area, Hunan Province, China. Respiratory specimens were screened for hMPV M gene by reverse-transcription polymerase chain reaction (RT-PCR). All RT-PCR positive' amplification products were confirmed by sequencing or analyzed and compared with those in GenBank known hMPV reference strain.hMPV was detected in 135 (5.2%) out of the 2613 children. The detected rate was significantly different between male and female (chi2 = 8.007, P = 0.003). The majority of the hMPV-positive patients (63.2%) were 0-1 year old infants. The majority of hMPV-positive patients were detected during spring season. Seasonal distribution showed that subtype A2b circulated predominantly in winter and spring, subtype B2 circulated predominantly in summer and spring. The most common genotype in 135 hMPV-positive patients were A and B in human subjects in Changsha. The predominant genotype of hMPV changed over the 3.5 year study period from genotype A2b to combined A2b and B2, and to B2. The 66 (48.9%) out of the 135 hMPV-positive patients were found to be co-infected with other respiratory viruses, and human bocavirus (HBoV) was the most common additional respiratory virus.These data suggest that some cases of acute lower respiratory infection in young children in Changsha area are related to hMPV and the majority of children infected with hMPV were less than 1 years old, the detected rate of boys was higher than that of girls. It circulated predominantly in spring. The subtype A2b genetic lineage and the subtype B1 genetic lineage of hMPV were revealed alternately in human subjects in Changsha area and the hMPV exhibited high co-infection rate with other respiratory viruses.
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- 2012
29. [Viral etiology and risk factors for severe community-acquired pneumonia in children]
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Xiao-Fang, Ding, Bing, Zhang, Li-Li, Zhong, Ni-Guang, Xiao, Qiong-Hua, Zhou, Zhao-Jun, Duan, Zhi-Ping, Xie, and Han-Chun, Gao
- Subjects
Community-Acquired Infections ,Male ,Logistic Models ,Risk Factors ,Child, Preschool ,Human bocavirus ,Pneumonia, Viral ,Infant, Newborn ,Humans ,Infant ,Female ,Respiratory Syncytial Viruses - Abstract
To study the virus spectrum of severe community-acquired pneumonia (CAP) and risk factors for the disease in children.Respiratory secretion specimens were collected from 1096 children hospitalized with CAP from June 2007 to November 2008, including 100 cases of severe CAP. Respiratory viruses were detected by PCR, nest-PCR or RT-PCR. Clinical data on the children were analyzed by univariate and multivariate logistic regression analysis for examining risk factors for severe CAP.Viral pathogens were isolated from 82 (82%) of the 100 cases with severe CAP. RSV was the most common (37%), followed by HBoV (25%) and HRV (18%). Mixed infection was noted in 32 cases (32%). The presence of underlying diseases (OR=6.623, P0.01) and RSV infection (OR=1.672, P0.05) were risk factors for severe CAP in children, while age was a protective factor (OR=0.475, P0.01).RSV is the most frequent viral pathogen in children with severe CAP. The presence of underlying diseases and RSV infection may be risk factors for severe CAP, while age is a protective factor.
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- 2012
30. [Viral etiology of 1165 hospitalized children with acute lower respiratory tract infection]
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Ni-Guang, Xiao, Bing, Zhang, Zhao-Jun, Duan, Zhi-Ping, Xie, Qiong-Hua, Zhou, Li-Li, Zhong, Han-Chun, Gao, Xiao-Fang, Ding, Sai-Zhen, Zeng, Han, Huang, and Yun-De, Hou
- Subjects
Male ,Adolescent ,Infant, Newborn ,Infant ,Age Distribution ,Child, Preschool ,Nasopharynx ,Viruses ,Humans ,Female ,Seasons ,Sex Distribution ,Child ,Child, Hospitalized ,Respiratory Tract Infections - Abstract
To explore the viral etiology of acute low respiratory tract infection (ALRTI) among hospitalized children in Changsha of Hunan Province of China.Nasopharyngeal aspirates were collected from 1165 hospitalized children with ALRTI in Changsha from September 2007 to August 2008. Respiratory syncytin virus (RSV), human rhinovirus (HRV), influenza virus A (IFVA), influenza virus B (IFVB), parainfluenza 1-3 (PIV 1-3), human metapneumovirus (hMPV), human coronaviruses NL63 (HCoV-NL63), and human coronaviruses HKU1 (HCoV-HKU1) were detected by reverse transcription polymerase chain reaction (RT-PCR). Adenovirus (ADV) and human bocavirus (HBoV) were detected by standard polymerase chain reaction (PCR). WU polyomaviruses (WUPyV) and KI polyomaviruses(KIPyV) were detected by nested PCR. The positive samples further underwent genetic sequencing.Among the 1165 nasopharyngeal aspirates, viruses were detected in 871 samples (74.76%), among which RSV (27.03%) was the most common virus, followed by HRV (17.33%), PIV3 (13.73%), HBoV (8.67%) and hMPV (6.52%). The overall positive rate of viral detection showed no significant differences between males and females (X2=2.241, P=0.134), whereas the positive rates of PIV3, hMPV, and HBoV in males were higher than in females. The positive rate of viral detection showed significant differences among different age groups (X2=10.934, P=0.027), and the highest positive rate was noted in the age group of 6 months to 1 year. Furthermore, the overall positive rate of viral detection showed a significant difference in term of seasonal distribution, with a peak prevalence in winter.Virues predominate in the etiology of pediatric ALRTI in Changsha, and RSV, HRV and PIV3 are the main viruses for ALRTI. HBoV and hMPV have become increasingly important. Viral infection-associated ALRTI shows a prevail in the age group of 6 months to 1 year as well as in winter.
- Published
- 2012
31. [Detection and clinical study on coronavirus HKU1 with acute lower respiratory tract infections of hospitalized children in Changsha]
- Author
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Ni-guang, Xiao, Zhi-ping, Xie, Qiong-hua, Zhou, Rong-fang, Zhang, Li-li, Zhong, Han-chun, Gao, Xiao-fang, Ding, Jia, Li, Jing-rong, Song, Yun-de, Hou, Bing, Zhang, and Zhao-jun, Duan
- Subjects
Coronavirus ,Male ,China ,Child, Preschool ,Acute Disease ,Infant, Newborn ,Humans ,Infant ,Female ,Child, Hospitalized ,Polymerase Chain Reaction ,Respiratory Tract Infections ,Phylogeny - Abstract
In order to understand the epidemiological and virologic characteristics of coronavirus HKU1 infection in hospitalized children with acute respiratory tract infection (ARTI) in Changsha.1165 nasopharyngeal aspirates (NPA) specimens were collected from hospitalized children with ARTI between September 2007 and August 2008 in Changsha. Specimens were screened for pol gene of coronavirus HKU1 by polymerase chain reaction. All positive amplification products were confirmed by sequencing and compared with those in GenBank.Coronavirus HKU1 were detected in 12 patients (1.03%) out of the 1165 children. The patients were from 8 days to 3 years. The most common clinical diagnosis was bronchopneumonia(83.33%). Similarity of coronavirus HKU1 with those published in the GenBank at nucleotide levels was 98.18% - 100%.Coronavirus HKU1 may be important pathogens in children with acute lower respiratory tract infection. Coronavirus HKU1 infections are common in children under 3 years old. There is no significant difference in the infectious rate between the boys and the girls. The peak of its prevalence is in spring and winter. A single genetic lineage of Coronavirus HKU1 was revealed in human subjects in Changsha.
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- 2011
32. [The research of saffold virus in children with lower respiratory tract infection in Changsha]
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Qiong-hua, Zhou, Bing, Zhang, Zhi-ping, Xie, Han-chun, Gao, Ming-wei, Guo, Fei, Zhang, Kun-long, Yan, Rong-fang, Zhang, Jing, Zhang, Chang-qing, Cao, and Zhao-jun, Duan
- Subjects
Male ,China ,Cardiovirus ,Adolescent ,Child, Preschool ,Infant, Newborn ,Humans ,Infant ,Female ,Child ,Polymerase Chain Reaction ,Respiratory Tract Infections - Abstract
To investigate prevalence of Saffold virus (SAFV) in Changsha area of hospitalized children with respiratory tract infection, and to discuss whether this virus is related to respiratory tract infection of children.643 nasopharyngeal aspirates samples were collected from hospitalized children with respiratory tract infection of the first affiliated hospital of Hunan nomal university during Nov. 2007 to Oct. 2008. Real-time fluorescent quanti-tative PCR(FQ-PCR) performed to screen the 5'UTR gene. And then analyze clinical data.SAFV were detected in 67 patients (10.42%) out of the 643 children, it was not detected over 5 years of age. The virus were detected in 8 patients (25.81%) out of the 31 children with persistent pneumonia and chronic pneumonia, there was statistically significant.There existed SAFV infection in hospitalized children with lower respiratory infection in Changsha area; SAFV maybe related to disease onset with lower respiratory tract infection of children.
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- 2011
33. [Detection and clinical characteristics analysis of human bocavirus 1-3 in children for acute respiratory infection in Lanzhou area]
- Author
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Chang-qing, Cao, Yu-ning, Li, Yu, Jin, Zhi-ping, Xie, Han-chun, Gao, Qiong-hua, Zhou, Xiao-qian, Gao, Ya-ting, Zhang, Jian, Zhang, and Zhao-jun, Duan
- Subjects
Male ,China ,Human bocavirus ,Acute Disease ,Humans ,Infant ,Female ,Respiratory Tract Infections ,Phylogeny - Abstract
To study the clinical and molecular epidemiology characteristics of human Bocavirus 1-3 (HBoV1-3) in children for acute respiratory infection in Lanzhou area.Nasopharyngeal aspiration samples and throat swabs were collected from 524 children with ARTI at the First Hospital of Lanzhou University, Gansu Province, China, between December 2009 and November 2010. Nested PCR was employed to screening HBoV1-3, which amplified a 518-bp fragment of the partial NS1 gene. Furthermore, a standard reverse transcription-PCR was used to screen for other common respiratory viruses.The overall frequency of HBoV was 8.2% (43/524), lining up behind human rhinovirus, RSV, parainfluenza virus 3. Thirty of the HBoV-postive children(69.8%) were co-infected with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously higher than that in AURI. The 2 HBoV2 NS1 sequences shared 99% and 100% nucleotide sequence identity with HBoV2 strain CU47TH respectively. Two cases of HBoV2 postive children appears gastrointestinal symptoms. The one HBoV3 NS1 sequences shared 99% nucleotide sequence identity with HBoV3 isolate 46-BJ07.The HBoV3 was detected at the first time in lanzhou area. HBoV1-3 infection exists in children with acute respiratory tract infections in Lanzhou region, HBoV1 were dominant. The mixed infection rate was higher.
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- 2011
34. [Molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in Nanjing City]
- Author
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Yu, Jin, Jing, Zhang, De-yu, Zhao, Zhi-ping, Xie, Han-chun, Gao, Xiao-qian, Gao, Ya-ting, Zhang, and Zhao-jun, Duan
- Subjects
Male ,China ,Reverse Transcriptase Polymerase Chain Reaction ,Child, Preschool ,Acute Disease ,Humans ,Infant ,Female ,Metapneumovirus ,Respiratory Tract Infections ,Phylogeny - Abstract
To investigate the molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in Nanjing city, China.Nasopharyngeal aspirates and nasopharyngeal swab were taken from 642 outpatients or hospitalized pediatric patients with acute at the Children Hospital of Nanjing, Jiangsu Province, China, between August 2009 and July 2010. Respiratory speciments were tested for the M gene of hMPV by reverse-transcription polymerase chain reaction (RT-PCR). All RT-PCR positive products were sequenced and phlogenetic analysis was conducted.hMPV was detected in 35 (5.5%) of the 642 children. Phylogenetic analysis revealed that 51.4% of the hMPV were B1, 31.4% were A2b. The peak of the positive rate was in April. The majority of the hMPV-positive patients(71.4%) were 0-1 years old. Of the 35 hMPV-positive patients, 15 (42.8%) were co-infected with other respiratory viruses, and human rhinovirus (HRV) were the most common additional respiratory virus. The most common clinical diagnosis was pneumonia (48.6%).Human metapneumovirus is an important pathogen of acute respiratory tract infection in children in Nanjing city. The subtype B1 was the predominating lineage in 2009-2010 in Nanjing city. No significant differences were found for clinical characteristics between genotype A and genotype B human metapneumovirus infection in children in Nanjing.
- Published
- 2011
35. [Molecular epidemiological and clinical features of coronavirus HKU1 in children with acute respiratory tract infection in Lanzhou]
- Author
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Jing-rong, Song, Yu, Jin, Zhao-jun, Duan, Xin-hui, Yuan, Kun-long, Yan, Yang, Zhao, Zhi-ping, Xie, and Han-chun, Gao
- Subjects
Coronavirus ,Male ,China ,Molecular Epidemiology ,Child, Preschool ,Infant, Newborn ,Humans ,Infant ,Female ,Child ,Respiratory Tract Infections - Abstract
Human coronavirus (CoV)-HKU1 (HCoV-HKU1) was first isolated by Woo et al in Hong Kong. Several successive reports confirmed retrospectively that this new human coronavirus was circulating in different countries worldwide. However, the impact and the role of the emerging HCoV-HKU1 were not defined in children with ARTI. The objective of this study was to investigate the molecular epidemiology and clinical characteristics of HCoV-HKU1 infection in children with ARTI in Lanzhou, China.Nasopharyngeal aspiration (NPA) samples were collected from 301 children with ARTI at the First Hospital of Lanzhou University, Gansu Province, China, between November 2007 and October 2008. Demographic data and clinical findings of these children were collected at the same time. The informed consent was obtained from their parents. This study protocol was approved by the hospital ethics committee. The reverse transcription polymerase chain reaction (RT-PCR) was employed to screen HCoV-HKU1. Furthermore, other common respiratory viruses were screened in HCoV-HKU1 positive samples. All PCR positive products were sequenced, and phylogenetic analysis was conducted.The overall frequency of HCoV-HKU1 infection was 5.0% (15/301). The HCoV-HKU1 pol gene sequences shared a 95.8% - 99.6% nucleotide identity with the human coronavirus-HKU1 strain, whereas the amino acid identity was 90.7% - 99.3%. The phylogenetic analysis revealed that the HCoV-HKU1 strain pol gene clustered with the HCoV-HKU1 strain N15 genotype B (no. DQ415911); 11 of 15 HCoV-HKU1 positive sample tested were mixed-infection. HCoV-HKU1 was detected only from November to April. Positive specimens peaked in November. Children with HCoV-HKU1 infection varied in age from 15 day to 12-years (median age, 10 months). The clinical diagnoses of HCoV-HKU1 positive patients included those with AURI and LURI. The clinical presentations of HCoV-HKU1 positive children included fever, cough, sputum production, diarrhea, vomiting; pharynx engorgement, crackles, and wheezing. The mean hospital stay of the 14 patients was 9.9 days. Six of 15 HCoV-HKU1 positive patients had an underlying illness, and they were all inpatients (hospital stay, mean, 11.2 days). There was no statistically significant difference in the detection rate between the two groups with and without underlying illnesses.Human CoV-HKU1 infection exists in children with respiratory tract infections in Lanzhou region. A single HCoV-HKU1 genotype B was circulating locally. The symptoms and clinical diagnoses of those infected with HCoV-HKU1 had no specificity as compared with patients with other common respiratory viruses infection.
- Published
- 2010
36. Human bocavirus infection in young children with acute respiratory tract infection in Lanzhou, China
- Author
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Li-shu Zheng, Yu Jin, Zhao-jun Duan, Zi-qian Xu, Xin-hui Yuan, Rong-fang Zhang, Yun-de Hou, Jing-rong Song, Han-chun Gao, and Zhi-ping Xie
- Subjects
Male ,China ,Adolescent ,Molecular Sequence Data ,human bocavirus ,Comorbidity ,Polymerase Chain Reaction ,Severity of Illness Index ,Virus ,Article ,Parvoviridae Infections ,Viral Proteins ,children ,Virology ,Nasopharynx ,medicine ,Prevalence ,Cluster Analysis ,Humans ,Respiratory system ,Child ,Acute respiratory tract infection ,Respiratory Tract Infections ,Phylogeny ,Polymorphism, Genetic ,biology ,business.industry ,Human bocavirus ,Respiratory disease ,Infant, Newborn ,Infant ,Sequence Analysis, DNA ,Viral Load ,acute respiratory tract infection ,biology.organism_classification ,medicine.disease ,coinfection ,Infectious Diseases ,medicine.anatomical_structure ,Child, Preschool ,Immunology ,Coinfection ,Female ,Seasons ,business ,Viral load ,Respiratory tract ,Research Article - Abstract
Human bocavirus (HBoV) is a recognized human parvovirus associated with acute respiratory tract infection. However, HBoV has yet to be established as a causative agent of respiratory disease. In this study, the epidemiological and virological characteristics of HBoV infection were studied in children with acute respiratory tract infection in China. In total, 406 children younger than 14 years of age with acute respiratory tract infection were included in this prospective 1‐year study. HBoV was detected in 29 (7.1%) of the 406 children. No clear seasonal fluctuation was observed in infection rates of HBoV. Of the 29 children infected with HBoV, 16 (55.2%) were coinfected with other respiratory viruses, most commonly respiratory syncytial virus (RSV). Viral coinfection with HBoV did not affect the severity of the respiratory disease (P = 0.291). The number of HBoV genome copies ranged from 5.80 × 102 to 9.72 × 108 copies/ml in nasopharyngeal aspirates among HBoV‐positive specimens by real‐time PCR, and neither coinfection nor the severity of disease correlated with the viral load (P = 0.148, P = 0.354, respectively). The most common clinical features were cough and acute upper respiratory infection, and acute bronchopneumonia. Additionally, the NP‐1 gene of HBoV showed minimal sequence variation. These data suggest that HBoV is frequent in young children with acute respiratory tract infection in Lanzhou, China, and RSV is the most common coinfecting virus. There was no apparent association between the viral load of HBoV and coinfection or disease severity. The NP‐1 gene was highly conserved in HBoV. J. Med. Virol. 82:282–288, 2010. © 2009 Wiley‐Liss, Inc.
- Published
- 2009
37. [Evaluation of reverse transcription loop-mediated isothermal amplification for detection of avian influenza A H5N1 virus]
- Author
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Qi-Ming, Li, Xue-Jun, Ma, Han-Chun, Gao, Rui, Zhou, Zhi-Zhou, Kuang, and Yun-De, Hou
- Subjects
Birds ,Influenza A Virus, H5N1 Subtype ,Reverse Transcriptase Polymerase Chain Reaction ,Influenza in Birds ,Animals ,Reproducibility of Results ,Nucleic Acid Amplification Techniques ,Sensitivity and Specificity - Abstract
A simple and sensitive Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) method was established to provide a new alternative for clinical diagnosis of Avian influenza A H5N1 virus. The method employed a set of six specially designed primers that recognized eight distinct sequences of the target for amplification of nucleic acid under isothermal conditions. In current study, fifty-one experimentally infected animal specimens and viral cultures that had been tested were analyzed by RT-LAMP for NA gene and HA gene, respectively. The amplification process of LAMP was monitored in real-time by the addition of SYBR Green dye. Meanwhile, the result showed high correlation between nested PCR and RT-LAMP. The specificity of the RT-LAMP assay was confirmed by restriction enzyme digestion analysis and sequencing of the amplified product. When the sensitivity of this assay was tested by serial 10-fold dilutions of RNA molecules from specimens, it was found that the RT-LAMP method achieved theoretically a sensitivity of 10 RNA molecules. Thus, we concluded that the RT-LAMP assay has potential usefulness for rapid detection of the Avian influenza A H5N1 virus.
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- 2008
38. Prevalence of Human KI and WU Polyomaviruses in Children with Acute Respiratory Tract Infection in China ▿
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Li-shu Zheng, Yu Jin, Xin-hui Yuan, Zhi-ping Xie, Zi-qian Xu, Jing-rong Song, Zhao-jun Duan, Rong-fang Zhang, Han-chun Gao, and Yun-De Hou
- Subjects
Microbiology (medical) ,Male ,medicine.medical_specialty ,China ,Molecular Sequence Data ,Biology ,Disease cluster ,Virology ,Nasopharynx ,Epidemiology ,medicine ,Prevalence ,Cluster Analysis ,Humans ,Respiratory system ,Child ,Acute respiratory tract infection ,Respiratory Tract Infections ,Phylogeny ,Polyomavirus Infections ,Respiratory tract infections ,Respiratory disease ,Infant, Newborn ,Infant ,Sequence Analysis, DNA ,medicine.disease ,Tumor Virus Infections ,medicine.anatomical_structure ,Child, Preschool ,Immunology ,DNA, Viral ,Female ,Polyomavirus ,Respiratory tract - Abstract
The KI and WU polyomaviruses were found in 11 (2.7%) and 17 (4.2%) of 406 nasopharyngeal aspirates, respectively, from children with acute respiratory tract infection (ARTI). The phylogenetic analysis indicates that they are all in the same cluster as the prototype strains. Our findings suggest that they are common in children with ARTI in China.
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- 2008
39. [Analysis of human metapneumovirus genotype in Hunan, China and its attachment protein G sequence character]
- Author
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Wen-pei, Liu, Bing, Zhang, Zhi-ping, Xie, Li-li, Zhong, Xiao-wang, Qu, Li-shu, Zheng, Han-chun, Gao, Zheng-yu, Qi, Zhi-zhou, Kuang, Wan-ju, Zhang, Zhou-jun, Duan, and Yun-de, Hou
- Subjects
China ,Viral Proteins ,Genotype ,Sequence Homology, Amino Acid ,Molecular Sequence Data ,Humans ,RNA, Viral ,Amino Acid Sequence ,Metapneumovirus ,Respiratory Syncytial Virus Infections ,Child ,Phylogeny ,Glycoproteins - Abstract
To understand the genotypes of human metapneumovirus (hMPV) and the genetic character of hMPV attachment protein G sequence in Hunan, China.232 nasopharyngeal aspirates (NPA) samples from hospitalized children with acute respiratory infections were collected from Hunan, China in 2005. HMPV was detected. The full length of G glycoprotein genes were amplified and sequenced. Bioinformatics soft-wares were employed to analyze the sequences.17/232 (7.3%) were showed hMPV positive. And co-infection rate with other viruses is 35%. The diagnoses of these hMPV positive cases are pneumonia, bronchiolitis and bronchopneumonia. Phylogenetic analysis for G genes from 13 hMPVs revealed the existence of four major subgroups: A1, A2, B1, B2 in Hunan, China in 2005. There are four types of sequence lengths of hMPV G glycoprotein, which are 711, 675, 660, 696nt. It is different in potential N-linked glycosylation sites and number of cysteine residues among these hMPVs of Hunan, China and Beijing, China. Also it is different from those in Japan and North America.The investigation of hMPV from Hunan, China in 2005 revealed the high speed of genetic variation and the marked character of geographic epidemic differences.
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- 2008
40. [WU polyomavirus and KI polyomavirus detected in specimens from children with acute respiratory tract infection in China]
- Author
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Xin-hui, Yuan, Zi-qian, Xu, Zhi-ping, Xie, Han-chun, Gao, Rong-fang, Zhang, Jing-rong, Song, Li-shu, Zheng, Dan-di, Li, Miao, Jin, Wei-xia, Cheng, Yu, Jin, Zhao-jun, Duan, and Yun-de, Hou
- Subjects
Male ,China ,Adolescent ,Infant, Newborn ,Infant ,Sequence Analysis, DNA ,Polymerase Chain Reaction ,Child, Preschool ,Humans ,Female ,Child ,Polyomavirus ,Respiratory Tract Infections ,Phylogeny - Abstract
To investigate newly identified polyomavirus WUV and WUV and KIPyV are associated with acute respiratory infections in China, tests were developed to detect WUV and KIPyV gene fragments from nasopharyngeal aspirates collected from children with ARI fron Nov. 2006 to Oct. 2007.A total of 318 clinical samples were tested for WUV and KIPyV using PCR method. The positive products were sequenced and compared with those in GenBank.14 of the 318 Samples were positive (WUV was 2.2%, KIPyV was 2.2%). All of children who were positive for WUV or KIPyV had respiratory illness.Polyomavirus WU and KIPyV infection may be associated with upper and lower respiratory diseases.
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- 2008
41. [Genome cloning and phylogenetic analysis of human bocavirus capsid gene]
- Author
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Zheng-Yu, Qi, Xiao-Wang, Qu, Wen-Pei, Liu, Zhi-Ping, Xie, Han-Chun, Gao, Li-Shu, Zheng, Zhi-Zhou, Kuang, Jian-Ping, Yu, and Zhao-Jun, Duan
- Subjects
Bocavirus ,Base Sequence ,Molecular Sequence Data ,Humans ,Capsid Proteins ,Amino Acid Sequence ,Genome, Viral ,Cloning, Molecular ,Polymerase Chain Reaction ,Conserved Sequence ,Phylogeny - Abstract
The full-length genome of one human bocavirus (HBoV) and the VP1 sequences of nine HBoV were amplified from patients' samples by PCR, cloned into pGEM-T vector separately, and sequenced. In this study, the one full length gemome and nine VP1 sequences of HBoV were aligened with 14 sequences of Parvoviruses which were canonical exemplars in Parvovirinae. Phylogenetic analysis showed that HBoV capsid sequences positioned closely to B19 parvovirus, although they positioned far in phylogenetic tree based on full length genome. Many similarities were found between HBoV and B19 in capsid by alignment on secondary structural elements. Because both B19 and HBoV are the only Parvoviruses that infect mankind, so study on HBoV may be used for reference to B19 which had been studied for about 30 years. By analysis of mutational sites, HBoV capsid protein showed a highly conserved secondary structural elements, but highly active in VP1-U, leading end of VP2 and insertions between the strands of the betaG-H. This cued that HBoV inclined to immune evasion and infectant adaptive faculty.
- Published
- 2007
42. [Biological activities of recombinant human interferon Epsilon]
- Author
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Fu-wang, Peng, Han-chun, Gao, Zhi-ping, Xie, Hui, Zhang, Qi-ming, Li, Zhao-jun, Duan, and Yun-de, Hou
- Subjects
Gene Expression ,Microbial Sensitivity Tests ,Antiviral Agents ,Recombinant Proteins ,Killer Cells, Natural ,Escherichia coli ,Humans ,Electrophoresis, Polyacrylamide Gel ,Interferons ,Cloning, Molecular ,K562 Cells ,Cell Proliferation ,HeLa Cells ,Plasmids - Abstract
To construct a novel recombinant rhIFN-epsilon155ser, and study its biological activities.The whole sequence of rhIFN-epsilon was artificially synthesized and some codons were altered according to the preferred codon using of E.coli. The sequence was cloned into plasmid vector pBV220 to express in E.coli DH5alpha. After purification and re-folding of rhIFN-epsilon155ser inclusion body, the final product was tested for its biological activities, including anti-viral, anti-proliferative and NK cell enhancing activities. At the same time, by using DNA microarray biochips, the gene expression patterns in the rhIFN-epsilon155ser and rhIFN-alpha2b treated cells were compared and analyzed.The re-built rhIFN-epsilon155ser sequence was expressed in E.coli as a form of inclusion body. After purified and re-folded, the rhIFN-epsilon155ser protein reached a purity of above 95%. The rhIFN-epsilon155ser protein had a specific anti-viral activity of about 6 x 10(5) IU/mg in WISH/VSV system. Its anti-proliferative activity and NK cell enhancing activities in vitro seemed to be lower than that of rhIFN-alpha2b. Data obtained from microarray biochips indicated that there were 283 pieces increasing 2 folds and 1489 pieces decreasing 2 folds among totally 22,278 pieces of human genes were found in the rhIFN-epsilon155ser treated cells; more changes in gene expression pattern were detected in the rhIFN-alpha treated cells.A novel recombinant rhIFN-epsilon155ser was constructed, which belonged to type 1 interferon. The biological activities of rhIFN-epsilon155ser were compared with rhIFN-alpha2b. The changes of gene expression pattern in the interferon treated cells were detected, analyzed and discussed.
- Published
- 2005
43. [Cloning, expression and purification of interferon-kappa, a novel human interferon, and its antiviral activity]
- Author
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Hui, Zhang, Zhao-jun, Duan, Jian-gao, Zhu, Fu-wang, Peng, Wu-ping, Li, Han-chun, Gao, Zhi-ping, Xie, Yu-e, Wang, and Yun-de, Hou
- Subjects
DNA, Complementary ,Gene Expression ,Microbial Sensitivity Tests ,Antiviral Agents ,Recombinant Proteins ,Cell Line ,Killer Cells, Natural ,Chlorocebus aethiops ,Interferon Type I ,Escherichia coli ,Animals ,Humans ,Electrophoresis, Polyacrylamide Gel ,Cloning, Molecular ,K562 Cells ,Vero Cells ,Cell Proliferation ,Plasmids - Abstract
To prepare human interferon-k (hIFN-kappa) and study its biological activities.Whole length of hIFN-kappa's cDNA was cloned, and its sequence was chemically synthesized according to the optimized codons of E.coli, then was expressed in E.coli DH5alpha. After purified, the rhIFN-kappa protein was tested for its various kinds of biological activities.The purity of rhIFN-kappa was above 90%. In WHIS-VSV system, the antiviral activity of rhIFN-kappa was 2.0 x 10(6) IU/mg. Compared with rhIFN-alpha-2b, the biological activities of rhIFN-kappa were all feeble, including antiviral activity, promoting NK cell activity and anti-proliferation activity.Antiviral activities of rhIFN-kappa on cell lines of different species are different, different viruses show different sensitivity to rhIFN-kappa.
- Published
- 2005
44. Outbreak of febrile illness caused by coxsackievirus A4 in a nursery school in Beijing, China.
- Author
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Jin-Song Li, Xiao-Gen Dong, Meng Qin, Zhi-Ping Xie, Han-Chun Gao, Jun-Yong Yang, Xiao-Xin Yang, Dan-Di Li, Jie Li, and Zhao-Jun Duan
- Subjects
PATHOGENICITY of enteroviruses ,COXSACKIEVIRUS diseases ,DISEASE outbreaks ,ETIOLOGY of diseases ,SCHOOLS ,DISEASE risk factors ,INFECTIOUS disease transmission - Abstract
Background: Coxsackievirus A4 (CV-A4) is classified as human enterovirus A according to its serotype. CV-A4, an etiological agent of hand, foot, and mouth disease, affects children worldwide and can circulate in closed environments such as schools and hospitals for long periods. Findings: An outbreak of febrile illness at a nursery school in Beijing, China, was confirmed to be caused by CV-A4. Phylogenetic analysis of the complete genome of the isolated strain showed that the virus belongs to the same cluster as the predominant CV-A4 strain in China. This outbreak was controlled by effective measures. Conclusions: The early identification of the pathogen and timely intervention may be the most critical factors in controlling an outbreak caused by CV-A4 in a preschool. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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45. Human Bocavirus Infection, People’s Republic of China
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Zheng-Yu Qi, Han-Chun Gao, Fu-wang Peng, Can-Ping Huang, Wen-Pei Liu, Xiao-Wang Qu, Zhi-Ping Xie, Zhao-jun Duan, Li-shu Zheng, and Yun-De Hou
- Subjects
Microbiology (medical) ,Male ,China ,Lineage (genetic) ,Epidemiology ,viruses ,Parvoviridae Infections ,human bocavirus ,lcsh:Medicine ,lcsh:Infectious and parasitic diseases ,Bocavirus ,children ,Lower respiratory tract infection ,Nasopharynx ,medicine ,Humans ,lcsh:RC109-216 ,Child ,Phylogeny ,biology ,Parvovirus ,Human bocavirus ,lcsh:R ,People's Republic ,Infant ,dispatch ,Viral Load ,biology.organism_classification ,medicine.disease ,Virology ,Infectious Diseases ,Child, Preschool ,DNA, Viral ,Female ,Viral load - Abstract
A newly identified parvovirus, human bocavirus (HBoV), was found in 21 (8.3%) of 252 nasopharyngeal aspirates from hospitalized children with lower respiratory tract infection in Hunan Province, People's Republic of China. Viral loads were 10(4) to 10(10) copies/mL. Phylogenetic analysis of the VP1 gene showed a single genetic lineage of HBoV worldwide.
- Published
- 2007
46. Prevalence of adenovirus in children with acute respiratory tract infection in Lanzhou, China.
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Yu Jin, Rong-fang Zhang, Zhi-ping Xie, Kun-long Yan, Han-chun Gao, Jing-rong Song, Xin-hui Yuan, Yun-de Hou, and Zhao-jun Duan
- Abstract
Background: Human adenovirus (HAdV) is an important agent causing respiratory tract infection in children. Information on the epidemiological and clinical features of HAdV is limited in children with acute respiratory tract infections (ARTIs) in China, especially those of a novel genotype, Ad55. Methods: In total, 1169 nasopharyngeal aspirates were collected from children younger than 14 years with ARTIs between November 2006 and November 2009. The polymerase chain reaction (PCR) was used to screen HAdVs. All PCR-positive products were sequenced. Results: 74 of 1169 (6.33%) specimens were positive for HAdVs. Among positive cases, AdV3 (58/74) was detected most frequently, followed by AdV11 (10/74), AdV2 (2/74), AdV7 (2/69), AdV6 (1/74), and AdV1 (1/74). AdV55 was found in one case. The incidence of HAdV infection peaked in children aged 3–7 years. The most common clinical diagnosis was upper respiratory infection, and the most common syndrome was fever and cough.The comparison of HAdV and RSV group revealed that Children infected with group AdV were significant older than children infected with group RSV, had more fever but less frequently wheezing, and cough, crackles, and cyanosis, The duration of hospitalization between the AdV group and RSV group was not significant, but a greater frequency of LRTIs was observed in RSV group. Conclusions: HAdV is an important viral agent in children with ARTIs in Lanzhou City, China. Multiple HAdV serotypes co-circulated with Ad3, which was predominant in this 3-year study. The novel AdV55 genotype was found in one case. No fixed seasonal rhythm could be identified. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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47. Newly identified respiratory viruses associated with acute lower respiratory tract infections in children in Lanzou, China, from 2006 to 2009
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Han-chun Gao, Yun-De Hou, Zhiqiang Xie, Rong-fang Zhang, Jing-rong Song, Zhao-jun Duan, K.-L. Yan, Xin-hui Yuan, Yu Jin, and Wei-xia Cheng
- Subjects
Microbiology (medical) ,Male ,China ,Adolescent ,viruses ,Article ,Viral genetics ,stomatognathic system ,respiratory viruses ,Acute lower respiratory tract infection ,Medicine ,Humans ,In patient ,Respiratory system ,Respiratory Tract Infections ,child ,Respiratory tract infections ,business.industry ,Incidence (epidemiology) ,Infant, Newborn ,Infant ,virus diseases ,General Medicine ,medicine.disease ,Virology ,Pneumonia ,Infectious Diseases ,Bronchiolitis ,Virus Diseases ,Child, Preschool ,DNA, Viral ,Viruses ,RNA, Viral ,Female ,business - Abstract
Nasopharyngeal aspirates were collected from 813 children
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