57 results on '"Gridley, Thomas"'
Search Results
2. Notch2 is required in somatic cells for breakdown of ovarian germ-cell nests and formation of primordial follicles.
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Xu, Jingxia and Gridley, Thomas
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SOMATIC cells , *INSECT reproduction , *OOGENESIS , *GERM cells , *GRANULOSA cells , *LABORATORY mice , *CELL proliferation - Abstract
Background: In the mouse ovary, oocytes initially develop in clusters termed germ-cell nests. Shortly after birth, these germ-cell nests break apart, and the oocytes individually become surrounded by somatic granulosa cells to form primordial follicles. Notch signaling plays essential roles during oogenesis in Drosophila, and recent studies have suggested that Notch signaling also plays an essential role during oogenesis and ovary development in mammals. However, no in vivo loss-of-function studies have been performed to establish whether Notch family receptors have an essential physiological role during normal ovarian development in mutant mice Results: Female mice with conditional deletion of the Notch2 gene in somatic granulosa cells of the ovary exhibited reduced fertility, accompanied by the formation of multi-oocyte follicles, which became hemorrhagic by 7 weeks of age. Formation of multi-oocyte follicles resulted from defects in breakdown of the primordial germ-cell nests. The ovaries of the Notch2 conditional mutant mice had increased numbers of oocytes, but decreased numbers of primordial follicles. Oocyte numbers in the Notch2 conditional mutants were increased not by excess or extended cellular proliferation, but as a result of decreased oocyte apoptosis. Conclusions: Our work demonstrates that Notch2-mediated signaling in the somatic-cell lineage of the mouse ovary regulates oocyte apoptosis non-cell autonomously, and is essential for regulating breakdown of germ-cell nests and formation of primordial follicles. This model provides a new resource for studying the developmental and physiological roles of Notch signaling during mammalian reproductive biology. [ABSTRACT FROM AUTHOR]
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- 2012
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3. Notch Signaling during Oogenesis in Drosophila melanogaster.
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Jingxia Xu and Gridley, Thomas
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OOGENESIS , *NOTCH genes , *CELLULAR signal transduction , *DROSOPHILA melanogaster , *INSECT reproduction , *EMBRYOLOGY , *CELL differentiation , *STEM cells - Abstract
The Notch signaling pathway is an evolutionarily conserved intercellular signaling mechanism that is required for embryonic development, cell fate specification, and stem cell maintenance. Discovered and studied initially in Drosophila melanogaster, the Notch pathway is conserved and functionally active throughout the animal kingdom. In this paper, we summarize the biochemical mechanisms of Notch signaling and describe its role in regulating one particular developmental pathway, oogenesis in Drosophila [ABSTRACT FROM AUTHOR]
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- 2012
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4. Snail1 Gene Function During Early Embryo Patterning in Mice.
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Murray, Stephen A. and Gridley, Thomas
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- 2006
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5. Snail family genes are required for left-right asymmetry determination, but not neural crest formation, in mice.
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Murray, Stephen A. and Gridley, Thomas
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GENES , *GENETIC repressors , *EPITHELIAL cells , *MESENCHYME , *MESODERM , *NEURAL crest , *SNAILS , *ASYMMETRY (Chemistry) - Abstract
Snail family genes encode zinc finger transcriptional repressors that are key regulators of epithelial-mesenchymal transitions in vertebrates, including the transitions that generate the mesoderm and neural crest. Here, we show that, contrary to observations in frog and avian embryos, the Snai1 family genes Snail (Snai1) and Slug (Snai2) are not required for formation and delamination of the neural crest in mice. However, embryos with conditional inactivation of Snail function exhibit defects in left-right asymmetry determination. This work demonstrates that although some aspects of Snail family gene function, such as a role in left-right asymmetry determination, appear to be evolutionarily conserved, their role in neural crest cell formation and delamination is not. [ABSTRACT FROM AUTHOR]
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- 2006
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6. Mutations in Snail Family Genes Enhance Craniosynostosis of Twist1 Haplo-insufficient Mice: Implications for Saethre-Chotzen Syndrome.
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Oram, Kathleen F. and Gridley, Thomas
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CRANIAL sutures , *CRANIOSYNOSTOSES , *SKULL abnormalities , *SNAILS , *GENETICS - Abstract
In Drosophila, mutations in the Twist gene interact with mutations in the Snail gene. We show that the mouse Twist1 mutations interacts with Snail1 and Snail2 mutations to enhance aberrant cranial suture fusion, demonstrating that genetic interactions between genes of the Twist and Snail families have been conserved during evolution. [ABSTRACT FROM AUTHOR]
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- 2005
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7. Cell movements during gastrulation: Snail dependent and independent pathways
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Ip, Y. Tony and Gridley, Thomas
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GASTRULATION , *SNAILS , *PROTEINS , *CELL motility - Abstract
The morphogenetic process of gastrulation requires multiple inputs and intricate coordination. Genetic analyses demonstrate critical roles of vertebrate and invertebrate Snail proteins in this process. Together with other regulatory molecules including Wnt and BMP, the Snail pathways specify cell fate and reorganize cellular machineries to coordinate morphological changes and cell movements during gastrulation. [Copyright &y& Elsevier]
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- 2002
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8. Defects in somite formation in lunatic fringe-deficient mice.
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Zhang, Nian and Gridley, Thomas
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SOMITE , *MICE , *VERTEBRATES , *NOTCH genes , *DEVELOPMENTAL biology , *GENETIC engineering - Abstract
Presents research which showed that mice homozygous for a targeted mutation of the lunatic fringe (Lfng) gene have defects in somite formation and anterior-posterior patterning of the somites. Development of segmentation in vertebrates; Role of the Notch signaling pathway; Fringe gene in Drosophila; Irregularities of somites; Results of marker analysis; Importance of Lfng encoding.
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- 1998
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9. Lightening up a notch: Notch regulation of energy metabolism.
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Gridley, Thomas and Kajimura, Shingo
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NOTCH genes , *BROWN adipose tissue , *HOMEOSTASIS , *GENE expression - Abstract
The article discusses a study conducted by P. Bi and colleagues on the multiple roles played by Notch signalling in the regulation of adipose browning and energy homeostasis in mammals. It mentions the deletion of gene encoding the Notch 1 receptor or the gene encoding the Notch signaling pathway primary transcriptional effector by the authors to assess the direct relationship between Notch signaling levels and brown adipose tissue (BAT).
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- 2014
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10. Vascular Biology: Vessel guidance.
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Gridley, Thomas
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VASCULAR endothelial growth factors , *NOTCH genes , *NEOVASCULARIZATION , *TUMOR blood vessels , *CANCER treatment - Abstract
The article presents a discovery of the new role of Notch pathway in vascular biology. An over view of the papers published in the journal on vascular endothelial growth factor (VEGF) and Notch signaling pathways and role for Dll 4 Notch signaling during vascular development, is presented. The new findings are supposed to help a better understanding of cancer and in the treatment of it.
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- 2007
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11. Laser surgery for mouse geneticists.
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Gridley, Thomas and Woychik, Rick
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LASER surgery , *GENETICISTS , *LABORATORY mice , *PHENOTYPES , *EMBRYONIC stem cell research , *MICROINJECTIONS , *ANIMAL mutation breeding - Abstract
The authors focus on the laser surgery on mice which are subjected to genetic testing. In order to accelerate phenotype analysis, mutant mice from embryonic stem cells are being generated. The eight cell microinjection procedure in two approaches is being suggested for production of homozygotes. The authors conclude that the need for cost effectiveness for breeding, phenotyping and maintaining the mice will be met by laser surgery.
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- 2007
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12. Kick it up a Notch: NOTCH1 activation in T-ALL
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Gridley, Thomas
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T cells , *LYMPHOBLASTIC leukemia , *PATIENTS , *GENETIC mutation , *ETIOLOGY of diseases - Abstract
While the human NOTCH1 gene initially was cloned as part of a translocation breakpoint in T cell acute lymphoblastic leukemia (T-ALL) tumors, this translocation is present in only a small percentage of T-ALL patients. A recent paper by demonstrates that novel types of activating mutations in the NOTCH1 gene occur in more than half of all T-ALL cases, implicating NOTCH1 as a major player in the etiology of T-ALL. [Copyright &y& Elsevier]
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- 2004
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13. Notch signaling during vascular development.
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Gridley, Thomas
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BLOOD-vessel abnormalities , *ENDOTHELIUM - Abstract
Comments on the article 'Vascular patterning defects associated with expression of activated Notch4 in embryonic endothelium,' by H. Uyttendaele, J. Ho, et al, published in the May 8, 2001, issue of the 'Proceedings of the National Academy of Sciences of the United States of America' journal.
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- 2001
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14. Notch signaling in vascular development and physiology.
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Gridley, Thomas
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NOTCH genes , *CELL communication , *CELL differentiation , *MUSCLE cells , *BLOOD vessels , *NEOVASCULARIZATION , *CARDIOVASCULAR diseases - Abstract
Notch signaling is an ancient intercellular signaling mechanism that plays myriad roles during vascular development and physiology in vertebrates. These roles include regulation of artery/vein differentiation in endothelial and vascular smooth muscle cells, regulation of blood vessel sprouting and branching during both normal development and tumor angiogenesis, and the differentiation and physiological responses of vascular smooth muscle cells. Defects in Notch signaling also cause inherited vascular and cardiovascular diseases. In this review, I summarize recent findings and discuss the growing relevance of Notch pathway modulation for therapeutic applications in disease. [ABSTRACT FROM AUTHOR]
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- 2007
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15. Snail family genes are required for left–right asymmetry determination but not neural crest formation in mice
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Murray, Stephen A. and Gridley, Thomas
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- 2006
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16. Notch, stroke and dementia.
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Gridley, Thomas
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NOTCH genes , *CEREBROVASCULAR disease , *GENETICS - Abstract
Offers background and analysis of a paper that provides important data linking the Notch signalling pathway to familial causes of stroke in humans. The familial syndrome, cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL); The study by Tournier-Lasserve and colleagues found in the October 24, 1996 issue of `Nature'; The possible relationship to Alzheimer's disease.
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- 1996
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17. The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life.
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Zander, Mark A., Cancino, Gonzalo I., Gridley, Thomas, Kaplan, David R., and Miller, Freda D.
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TRANSCRIPTION factors , *SNAILS , *PROTEIN precursors , *NEURONS , *CYTOLOGY , *NEURAL stem cells , *MOLECULAR biology - Abstract
The Snail transcription factor regulates diverse aspects of stem cell biology in organisms ranging from Drosophila to mammals. Here we have asked whether it regulates the biology of neural precursor cells (NPCs) in the forebrain of postnatal and adult mice, taking advantage of a mouse containing a floxed Snail allele (Snailfl/fl mice). We show that when Snail is inducibly ablated in the embryonic cortex, this has long-term consequences for cortical organization. In particular, when Snailfl/fl mice are crossed to Nestin-cre mice that express Cre recombinase in embryonic neural precursors, this causes inducible ablation of Snail expression throughout the postnatal cortex. This loss of Snail causes a decrease in proliferation of neonatal cortical neural precursors and mislocalization and misspecification of cortical neurons. Moreover, these precursor phenotypes persist into adulthood. Adult neural precursor cell proliferation is decreased in the forebrain subventricular zone and in the hippocampal dentate gyrus, and this is coincident with a decrease in the number of adult-born olfactory and hippocampal neurons. Thus, Snail is a key regulator of the numbers of neural precursors and newborn neurons throughout life. [ABSTRACT FROM AUTHOR]
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- 2014
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18. Mmp15 is a direct target of Snai1 during endothelial to mesenchymal transformation and endocardial cushion development
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Tao, Ge, Levay, Agata K., Gridley, Thomas, and Lincoln, Joy
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GENE targeting , *ENDOTHELIUM , *HEART valves , *MORPHOGENESIS , *EMBRYOLOGY , *ZINC-finger proteins , *TRANSCRIPTION factors - Abstract
Abstract: Cardiac valves originate from endocardial cushions (EC) formed by endothelial-to-mesenchymal transformation (EMT) during embryogenesis. The zinc-finger transcription factor Snai1 has previously been reported to be important for EMT during organogenesis, yet its role in early valve development has not been directly examined. In this study we show that Snai1 is highly expressed in endothelial, and newly transformed mesenchyme cells during EC development. Mice with targeted snai1 knockdown display hypocellular ECs at E10.5 associated with decreased expression of mesenchyme cell markers and downregulation of the matrix metalloproteinase (mmp) family member, mmp15. Snai1 overexpression studies in atrioventricular canal collagen I gel explants indicate that Snai1 is sufficient to promote mmp15 expression, cell transformation, and mesenchymal cell migration and invasion. However, treatment with the catalytically active form of MMP15 promotes cell motility, and not transformation. Further, we show that Snai1-mediated cell migration requires MMP activity, and caMMP15 treatment rescues attenuated migration defects observed in murine ECs following snai1 knockdown. Together, findings from this study reveal previously unappreciated mechanisms of Snai1 for the direct regulation of MMPs during EC development. [Copyright &y& Elsevier]
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- 2011
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19. Patent ductus arteriosus in mice with smooth muscle-specific Jag1 deletion.
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Xuesong Feng,, Krebs, Luke T., and Gridley, Thomas
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DUCTUS arteriosus , *BLOOD vessels , *FETUS , *CONGENITAL heart disease , *LIGANDS (Biochemistry) , *SMOOTH muscle - Abstract
The ductus arteriosus is an arterial vessel that shunts blood flow away from the lungs during fetal life, but normally occludes after birth to establish the adult circulation pattern. Failure of the ductus arteriosus to close after birth is termed patent ductus arteriosus and is one of the most common congenital heart defects. Mice with smooth muscle cell-specific deletion of Jag1, which encodes a Notch ligand, die postnatally from patent ductus arteriosus. These mice exhibit defects in contractile smooth muscle cell differentiation in the vascular wall of the ductus arteriosus and adjacent descending aorta. These defects arise through an inability to propagate the JAG1-Notch signal via lateral induction throughout the width of the vascular wall. Both heterotypic endothelial smooth muscle cell interactions and homotypic vascular smooth muscle cell interactions are required for normal patterning and differentiation of the ductus arteriosus and adjacent descending aorta. This new model for a common congenital heart defect provides novel insights into the genetic programs that underlie ductus arteriosus development and closure. [ABSTRACT FROM AUTHOR]
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- 2010
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20. Epiblast-specifìc Snail deletion results in embryonic lethality due to multiple vascular defects.
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Lomelí, Hilda, Starling, Christa, and Gridley, Thomas
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GENE expression , *ZINC-finger proteins , *GENETIC regulation , *VERTEBRATES , *EMBRYOLOGY , *BLOOD flow , *BIOMOLECULES , *HEMODYNAMICS , *BLOOD circulation - Abstract
Background: Members of the Snail gene family, which encode zinc finger proteins that function as transcriptional repressors, play essential roles during embryonic development in vertebrates. Mouse embryos with conditional deletion of the Snail1 (Snai1) gene in the epiblast, but not in most extraembryonic membranes, exhibit defects in left-right asymmetry specification and migration of mesoderm cells through the posterior primitive streak. Here we describe phenotypic defects that result in death of the mutant embryos by 9.5 days of gestation. Findings: Endothelial cells differentiated in epiblast-specific Snai1-deficient embryos, but formation of an interconnected vascular network was abnormal. To determine whether the observed vascular defects were dependent on disruption of blood flow, we analyzed vascular remodeling in cultured allantois explants from the mutant embryos. Similar vascular defects were observed in the mutant allantois explants. Conclusion: These studies demonstrate that lethality in the Snai1-conditional mutant embryos is caused by multiple defects in the cardiovascular system. [ABSTRACT FROM AUTHOR]
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- 2009
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21. Notch Signaling Regulates Bile Duct Morphogenesis in Mice.
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Lozier, Julie, McCright, Brent, and Gridley, Thomas
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HUMAN abnormalities , *GENETIC mutation , *BILE duct diseases , *MORPHOGENESIS , *LABORATORY mice , *NOTCH genes , *CELL determination , *GENETIC carriers , *GENE frequency - Abstract
Background: Alagille syndrome is a developmental disorder caused predominantly by mutations in the Jagged1 (JAG1) gene, which encodes a ligand for Notch family receptors. A characteristic feature of Alagille syndrome is intrahepatic bile duct paucity. We described previously that mice doubly heterozygous for Jag1 and Notch2 mutations are an excellent model for Alagille syndrome. However, our previous study did not establish whether bile duct paucity in Jag1/Notch2 double heterozygous mice resulted from impaired differentiation of bile duct precursor cells, or from defects in bile duct morphogenesis. Methodology/Principal Findings: Here we characterize embryonic biliary tract formation in our previously described Jag1/Notch2 double heterozygous Alagille syndrome model, and describe another mouse model of bile duct paucity resulting from liver-specific deletion of the Notch2 gene. Conclusions/Significance: Our data support a model in which bile duct paucity in Notch pathway loss of function mutant mice results from defects in bile duct morphogenesis rather than cell fate specification. [ABSTRACT FROM AUTHOR]
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- 2008
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22. The Notch Ligand JAG1 Is Required for Sensory Progenitor Development in the Mammalian Inner Ear.
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Kiernan, Amy E., Jingxia Xu, Gridley, Thomas, and Beier, David
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INNER ear , *HAIR cells , *SENSE organs , *CELL differentiation , *GENE targeting , *CELL cycle - Abstract
In mammals, six separate sensory regions in the inner ear are essential for hearing and balance function. Each sensory region is made up of hair cells, which are the sensory cells, and their associated supporting cells, both arising from a common progenitor. Little is known about the molecular mechanisms that govern the development of these sensory organs. Notch signaling plays a pivotal role in the differentiation of hair cells and supporting cells by mediating lateral inhibition via the ligands Delta-like 1 and Jagged (JAG) 2. However, another Notch ligand, JAG1, is expressed early in the sensory patches prior to cell differentiation, indicating that there may be an earlier role for Notch signaling in sensory development in the ear. Here, using conditional gene targeting, we show that the Jag1 gene is required for the normal development of all six sensory organs within the inner ear. Cristae are completely lacking in Jag1-conditional knockout (cko) mutant inner ears, whereas the cochlea and utricle show partial sensory development. The saccular macula is present but malformed. Using SOX2 and p27kip1 as molecular markers of the prosensory domain, we show that JAG1 is initially expressed in all the prosensory regions of the ear, but becomes down-regulated in the nascent organ of Corti by embryonic day 14.5, when the cells exit the cell cycle and differentiate. We also show that both SOX2 and p27kip1 are down-regulated in Jag1-cko inner ears. Taken together, these data demonstrate that JAG1 is expressed early in the prosensory domains of both the cochlear and vestibular regions, and is required to maintain the normal expression levels of both SOX2 and p27kip1. These data demonstrate that JAG1-mediated Notch signaling is essential during early development for establishing the prosensory regions of the inner ear. [ABSTRACT FROM AUTHOR]
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- 2006
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23. Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells.
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Anthony, Todd E., Mason, Heather A., Gridley, Thomas, Fishell, Gord, and Heintz, Nathaniel
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NEUROGLIA , *GENES , *NEURONS , *NOTCH genes , *PROTEINS - Abstract
Radial glia function during CNS development both as neural progenitors and as a scaffolding supporting neuronal migration. To elucidate pathways involved in these functions, we mapped in vivo the promoter for Blbp, a radial glial gene. We show here that a binding site for the Notch effector CBF1 is essential for all Blbp transcription in radial glia, and that BLBP expression is significantly reduced in the forebrains of mice lacking the Notch1 and Notch3 receptors. These results identify Blbp as the first predominantly CNS-specific Notch target gene and suggest that it mediates some aspects of Notch signaling in radial glia. [ABSTRACT FROM AUTHOR]
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- 2005
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24. Role of the Notch signalling pathway in tooth morphogenesis
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Mitsiadis, Thimios A., Regaudiat, Laure, and Gridley, Thomas
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LABORATORY mice , *EPITHELIAL cells , *TEETH abnormalities , *DENTISTRY - Abstract
Summary: Notch receptors are involved in cell fate decisions through the process of lateral inhibition or inductive signalling. Jagged2 belongs to the family of transmembrane proteins that serve as the ligands for Notch receptors. We have analysed the expression of the Jagged2 gene in developing mouse teeth. Jagged2 expression is restricted in inner enamel epithelial cells that give rise to the ameloblasts. We have also examined the role of Jagged2 in tooth development using mutant mice that lack the domain of the Jagged2 protein required for interaction with the Notch receptors (DSL domain). Homozygous mutant mice die after birth, exhibit abnormal tooth morphology and fusions between the palatal and mandibular shelves. These results demonstrate that Notch signalling plays an essential role in tooth development. [Copyright &y& Elsevier]
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- 2005
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25. Notch1 and Notch2 collaboratively maintain radial glial cells in mouse neurogenesis.
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Mase, Shun, Shitamukai, Atsunori, Wu, Quan, Morimoto, Mitsuru, Gridley, Thomas, and Matsuzaki, Fumio
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NEUROGLIA , *NEURAL stem cells , *NOTCH genes , *DEVELOPMENTAL neurobiology , *MICE - Abstract
[Display omitted] • Notch signaling is essential to maintain radial glial cells (RGCs). • The functional relationship between Notch1 and Notch2 in RGCs is elusive. • Notch1 knockout affected RGC maintenance in early to mid-neurogenesis. • Notch1 and Notch2 function together for RGC maintenance in late neurogenesis. During mammalian corticogenesis, Notch signaling is essential to maintain neural stem cells called radial glial cells (RGCs) and the cortical architecture. Because the conventional knockout of either Notch1 or Notch2 causes a neuroepithelial loss prior to neurogenesis, their functional relationship in RGCs remain elusive. Here, we investigated the impacts of single knockout of Notch1 and Notch2 genes, and their conditional double knockout (DKO) on mouse corticogenesis. We demonstrated that Notch1 single knockout affected RGC maintenance in early to mid-neurogenesis whereas Notch2 knockout caused no apparent defect. In contrast, Notch2 plays a role in the RGC maintenance as Notch1 does at the late stage. Notch1 and Notch2 DKO resulted in the complete loss of RGCs, suggesting their cooperative function. We found that Notch activity in RGCs depends on the Notch gene dosage irrespective of Notch1 or Notch2 at late neurogenic stage, and that Notch1 and Notch2 have a similar activity, most likely due to a drastic increase in Notch 2 transcription. Our results revealed that Notch1 has an essential role in establishing the RGC pool during the early stage, whereas Notch1 and Notch2 subsequently exhibit a comparable function for RGC maintenance and neurogenesis in the late neurogenic period in the mouse telencephalon. [ABSTRACT FROM AUTHOR]
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- 2021
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26. Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
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Basch, Martin L., Brown II, Rogers M., Jen, Hsin-I, Semerci, Fatih, Depreux, Frederic, Edlund, Renée K., Hongyuan Zhang, Norton, Christine R., Gridley, Thomas, Cole, Susan E., Doetzlhofer, Angelika, Maletic-Savatic, Mirjana, Segil, Neil, and Groves, Andrew K.
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NOTCH signaling pathway , *CELLULAR signal transduction , *CORTI'S organ , *HAIR cells , *COCHLEA physiology , *PHYSIOLOGY - Abstract
The signals that induce the organ of Corti and define its boundaries in the cochlea are poorly understood. We show that two Notch modifiers, Lfng and Mfng, are transiently expressed precisely at the neural boundary of the organ of Corti. Cre-Lox fate mapping shows this region gives rise to inner hair cells and their associated inner phalangeal cells. Mutation of Lfng and Mfng disrupts this boundary, producing unexpected duplications of inner hair cells and inner phalangeal cells. This phenotype is mimicked by other mouse mutants or pharmacological treatments that lower but not abolish Notch signaling. However, strong disruption of Notch signaling causes a very different result, generating many ectopic hair cells at the expense of inner phalangeal cells. Our results show that Notch signaling is finely calibrated in the cochlea to produce precisely tuned levels of signaling that first set the boundary of the organ of Corti and later regulate hair cell development. [ABSTRACT FROM AUTHOR]
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- 2016
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27. Multiple functions of Snail family members in palate development and craniofacial morphogenesis
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Murray, Stephen A., Oram, Kathleen F., and Gridley, Thomas
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- 2007
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28. Parent stem cells can serve as niches for their daughter cells.
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Pardo-Saganta, Ana, Tata, Purushothama Rao, Law, Brandon M., Saez, Borja, Chow, Ryan Dz-Wei, Prabhu, Mythili, Gridley, Thomas, and Rajagopal, Jayaraj
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STEM cell niches , *HEMATOPOIETIC stem cells , *STEM cell research , *CELLULAR control mechanisms , *CELLULAR signal transduction , *CELL differentiation - Abstract
Stem cells integrate inputs from multiple sources. Stem cell niches provide signals that promote stem cell maintenance, while differentiated daughter cells are known to provide feedback signals to regulate stem cell replication and differentiation. Recently, stem cells have been shown to regulate themselves using an autocrine mechanism. The existence of a 'stem cell niche' was first postulated by Schofield in 1978 to define local environments necessary for the maintenance of haematopoietic stem cells. Since then, an increasing body of work has focused on defining stem cell niches. Yet little is known about how progenitor cell and differentiated cell numbers and proportions are maintained. In the airway epithelium, basal cells function as stem/progenitor cells that can both self-renew and produce differentiated secretory cells and ciliated cells. Secretory cells also act as transit-amplifying cells that eventually differentiate into post-mitotic ciliated cells . Here we describe a mode of cell regulation in which adult mammalian stem/progenitor cells relay a forward signal to their own progeny. Surprisingly, this forward signal is shown to be necessary for daughter cell maintenance. Using a combination of cell ablation, lineage tracing and signalling pathway modulation, we show that airway basal stem/progenitor cells continuously supply a Notch ligand to their daughter secretory cells. Without these forward signals, the secretory progenitor cell pool fails to be maintained and secretory cells execute a terminal differentiation program and convert into ciliated cells. Thus, a parent stem/progenitor cell can serve as a functional daughter cell niche. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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29. Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium.
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Horvay, Katja, Jardé, Thierry, Casagranda, Franca, Perreau, Victoria M, Haigh, Katharina, Nefzger, Christian M, Akhtar, Reyhan, Gridley, Thomas, Berx, Geert, Haigh, Jody J, Barker, Nick, Polo, Jose M, Hime, Gary R, and Abud, Helen E
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SNAILS , *STEM cells , *EPITHELIAL cells , *INTESTINAL cancer , *APOPTOSIS , *KNOCKOUT mice - Abstract
Snail family members regulate epithelial-to-mesenchymal transition ( EMT) during invasion of intestinal tumours, but their role in normal intestinal homeostasis is unknown. Studies in breast and skin epithelia indicate that Snail proteins promote an undifferentiated state. Here, we demonstrate that conditional knockout of Snai1 in the intestinal epithelium results in apoptotic loss of crypt base columnar stem cells and bias towards differentiation of secretory lineages. In vitro organoid cultures derived from Snai1 conditional knockout mice also undergo apoptosis when Snai1 is deleted. Conversely, ectopic expression of Snai1 in the intestinal epithelium in vivo results in the expansion of the crypt base columnar cell pool and a decrease in secretory enteroendocrine and Paneth cells. Following conditional deletion of Snai1, the intestinal epithelium fails to produce a proliferative response following radiation-induced damage indicating a fundamental requirement for Snai1 in epithelial regeneration. These results demonstrate that Snai1 is required for regulation of lineage choice, maintenance of CBC stem cells and regeneration of the intestinal epithelium following damage. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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30. The Snail Family Gene Snai3 Is Not Essential for Embryogenesis in Mice.
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Bradley, Cara K., Norton, Christine R., Chen, Ying, Han, Xianghua, Booth, Carmen J., Yoon, Jeong Kyo, Krebs, Luke T., and Gridley, Thomas
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MICE embryology , *TRANSCRIPTION factors , *GENETIC repressors , *CANCER invasiveness , *MORPHOGENESIS , *DEVELOPMENTAL biology , *METASTASIS - Abstract
The Snail gene family encodes zinc finger-containing transcriptional repressor proteins. Three members of the Snail gene family have been described in mammals, encoded by the Snai1, Snai2, and Snai3 genes. The function of the Snai1 and Snai2 genes have been studied extensively during both vertebrate embryogenesis and tumor progression and metastasis, and play critically important roles during these processes. However, little is known about the function of the Snai3 gene and protein. We describe here generation and analysis of Snai3 conditional and null mutant mice. We also generated an EYFP-tagged Snai3 null allele that accurately reflects endogenous Snai3 gene expression, with the highest levels of expression detected in thymus and skeletal muscle. Snai3 null mutant homozygous mice are viable and fertile, and exhibit no obvious phenotypic defects. These results demonstrate that Snai3 gene function is not essential for embryogenesis in mice. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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31. Blockade of individual Notch ligands and receptors controls graft-versus-host disease.
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Tran, Ivy T, Sandy, Ashley R, Carulli, Alexis J, Ebens, Christen, Chung, Jooho, Shan, Gloria T, Radojcic, Vedran, Friedman, Ann, Gridley, Thomas, Shelton, Amy, Reddy, Pavan, Samuelson, Linda C, Yan, Minhong, Siebel, Christian W, and Maillard, Ivan
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GRAFT versus host disease prevention , *T cells , *ANIMAL experimentation , *BONE marrow transplantation , *CELL physiology , *CELL receptors , *CELLULAR signal transduction , *DIARRHEA , *GRAFT versus host disease , *GROWTH factors , *HETEROCYCLIC compounds , *HOMOGRAFTS , *IMMUNOGLOBULINS , *INTERFERONS , *INTERLEUKIN-2 , *INTESTINES , *MEMBRANE proteins , *MICE , *PROTEOLYTIC enzymes , *REGENERATION (Biology) , *RESEARCH funding , *CHEMICAL inhibitors , *PHYSIOLOGY - Abstract
Graft-versus-host disease (GVHD) is the main complication of allogeneic bone marrow transplantation. Current strategies to control GVHD rely on global immunosuppression. These strategies are incompletely effective and decrease the anticancer activity of the allogeneic graft. We previously identified Notch signaling in T cells as a new therapeutic target for preventing GVHD. Notch-deprived T cells showed markedly decreased production of inflammatory cytokines, but normal in vivo proliferation, increased accumulation of regulatory T cells, and preserved anticancer effects. Here, we report that γ-secretase inhibitors can block all Notch signals in alloreactive T cells, but lead to severe on-target intestinal toxicity. Using newly developed humanized antibodies and conditional genetic models, we demonstrate that Notch1/Notch2 receptors and the Notch ligands Delta-like1/4 mediate all the effects of Notch signaling in T cells during GVHD, with dominant roles for Notch1 and Delta-like4. Notch1 inhibition controlled GVHD, but led to treatment-limiting toxicity. In contrast, Delta-like1/4 inhibition blocked GVHD without limiting adverse effects while preserving substantial anticancer activity. Transient blockade in the peritransplant period provided durable protection. These findings open new perspectives for selective and safe targeting of individual Notch pathway components in GVHD and other T cell-mediated human disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
32. Blockade of individual Notch ligands and receptors controls graft-versus-host disease.
- Author
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Tran, Ivy T., Sandy, Ashley R., Carulli, Alexis J., Ebens, Christen, Chung, Jooho, Shan, Gloria T., Radojcic, Vedran, Friedman, Ann, Gridley, Thomas, Shelton, Amy, Reddy, Pavan, Samuelson, Linda C., Minhong Yan, Siebel, Christian W., and Maillard, Ivan
- Subjects
- *
GRAFT versus host disease , *BONE marrow transplant complications , *IMMUNOSUPPRESSION , *CELLULAR signal transduction , *T cells , *CYTOKINES , *SECRETASE inhibitors , *CELL-mediated cytotoxicity - Abstract
Graft-versus-host disease (GVHD) is the main complication of allogeneic bone marrow transplantation. Current strategies to control GVHD rely on global immunosuppression. These strategies are incompletely effective and decrease the anticancer activity of the allogeneic graft. We previously identified Notch signaling in T cells as a new therapeutic target for preventing GVHD. Notch-deprived T cells showed markedly decreased production of inflammatory cytokines, but normal in vivo proliferation, increased accumulation of regulatory T cells, and preserved anticancer effects. Here, we report that γ-secretase inhibitors can block all Notch signals in alloreactive T cells, but lead to severe on-target intestinal toxicity. Using newly developed humanized antibodies and conditional genetic models, we demonstrate that Notch1/Notch2 receptors and the Notch ligands Delta-like1/4 mediate all the effects of Notch signaling in T cells during GVHD, with dominant roles for Notch1 and Delta-like4. Notch1 inhibition controlled GVHD, but led to treatment-limiting toxicity. In contrast, Delta-like1/4 inhibition blocked GVHD without limiting adverse effects while preserving substantial anticancer activity. Transient blockade in the peritransplant period provided durable protection. These findings open new perspectives for selective and safe targeting of individual Notch pathway components in GVHD and other T cell--mediated human disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
33. Blockade of individual Notch ligands and receptors controls graft-versus-host disease.
- Author
-
Tran, Ivy T., Sandy, Ashley R., Carulli, Alexis J., Ebens, Christen, Jooho Chung, Shan, Gloria T., Radojcic, Vedran, Friedman, Ann, Gridley, Thomas, Shelton, Amy, Reddy, Pavan, Samuelson, Linda C., Minhong Yan, Siebel, Christian W., and Maillard, Ivan
- Abstract
Graft-versus-host disease (GVHD) is the main complication of allogeneic bone marrow transplantation. Current strategies to control GVHD rely on global immunosuppression. These strategies are incompletely effective and decrease the anticancer activity of the allogeneic graft. We previously identified Notch signaling in T cells as a new therapeutic target for preventing GVHD. Notch-deprived T cells showed markedly decreased production of inflammatory cytokines, but normal in vivo proliferation, increased accumulation of regulatory T cells, and preserved anticancer effects. Here, we report that γ-secretase inhibitors can block all Notch signals in alloreactive T cells, but lead to severe on-target intestinal toxicity. Using newly developed humanized antibodies and conditional genetic models, we demonstrate that Notch1/Notch2 receptors and the Notch ligands Delta-like1/4 mediate all the effects of Notch signaling in T cells during GVHD, with dominant roles for Notch1 and Delta-like4. Notch1 inhibition controlled GVHD, but led to treatment-limiting toxicity. In contrast, Delta-like1/4 inhibition blocked GVHD without limiting adverse effects while preserving substantial anticancer activity. Transient blockade in the peritransplant period provided durable protection. These findings open new perspectives for selective and safe targeting of individual Notch pathway components in GVHD and other T cell-mediated human disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
34. Jagged1-mediated Notch signaling regulates mammalian inner ear development independent of lateral inhibition.
- Author
-
Hao, Jin, Koesters, Robert, Bouchard, Maxime, Gridley, Thomas, Pfannenstiel, Susanna, Plinkert, Peter K., Zhang, Luo, and Praetorius, Mark
- Subjects
- *
INNER ear , *HAIR cells , *SENSITIVITY analysis , *GENE targeting , *BRAIN stem , *GENE expression , *ANIMAL experimentation , *GENETICS , *MAMMALS , *MICE , *PROTEINS , *RESEARCH funding , *DATA analysis software , *MANN Whitney U Test - Abstract
Conclusion: Jagged1-mediated Notch signaling regulates hair cell (HC) production in a distinct way rather than lateral inhibition mediated by Hes1 and Hes5. Jagged1 may interact with Notch3, probably via candidate downstream mediators Hesr1 and Hesr2, regulating the prosensory formation in the early stage. Objectives: To explore the function of the Jagged1-mediated Notch signaling pathway in mammalian inner ear development and its possible mechanism. Methods: Using conditional gene targeting, a novel Jagged1 conditional knockout (Jag1-cko), Pax8cre/+; Jag1flox/flox, was established. The auditory brainstem response and swim ability test were utilized to identify functional disability. The expression of Jagged1, Notch3, Hes1, Hesr1, and Hesr2 was detected by immunofluorescence and immunohistochemistry. Results: Our Jag1-cko model was established and survived well. It presented hearing impairment and balance disturbance with 'waltzing' behavior. Cochleae and vestibular apparatus were all found in our Jag1-cko model. Patch deficiency of outer hair cells (OHCs) was found on the apical and middle turns of the auditory epithelium. OHCs were totally missing on the basal turn. The stereociliary bundles were disorientated on the cristae. Unlike Hes1, no expression of Notch3, Hesr1, and Hesr2 was found on embryonic day 13.5 of the Jag1-cko model. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
35. Conditional Deletion of Notch1 and Notch2 Genes in Excitatory Neurons of Postnatal Forebrain Does Not Cause Neurodegeneration or Reduction of Notch mRNAs and Proteins.
- Author
-
Jin Zheng, Watanabe, Hirotaka, Wines-Samuelson, Mary, Huailong Zhao, Gridley, Thomas, Kopan, Raphael, and Jie Shen
- Subjects
- *
NOTCH genes , *PROSENCEPHALON , *MESSENGER RNA , *SCISSION (Chemistry) , *INTRACELLULAR membranes , *NEUROPLASTICITY - Abstract
Activation of Notch signaling requires intramembranous cleavage by γ-secretase to release the intracellular domain. We previously demonstrated that presenilin and nicastrin, components of the γ-secretase complex, are required for neuronal survival in the adult cerebral cortex. Here we investigate whether Notch1 and/or Notch2 are functional targets of presenilin/γ-secretase in promoting survival of excitatory neurons in the adult cerebral cortex by generating Notch1, Notch2, and Notch1/Notch2 conditional knock-out (cKO) mice. Unexpectedly, we did not detect any neuronal degeneration in the adult cerebral cortex of these Notch cKO mice up to ∼2 years of age, whereas conditional inactivation of presenilin or nicastrin using the same αCaMKII-Cre transgenic mouse caused progressive, striking neuronal loss beginning at 4 months of age. More surprisingly, we failed to detect any reduction of Notch1 and Notch2 mRNAs and proteins in the cerebral cortex of Notch1 and Notch2 cKO mice, respectively, even though Cre-mediated genomic deletion of the floxed Notch1 and Notch2 exons clearly took place in the cerebral cortex of these cKO mice. Furthermore, introduction of Cre recombinase into primary cortical cultures prepared from postnatal floxed Notch1/Notch2 pups, where Notch1 and Notch2 are highly expressed, completely eliminated their expression, indicating that the floxed Notch1 and Notch2 alleles can be efficiently inactivated in the presence of Cre. Together, these results demonstrate that Notch1 and Notch2 are not involved in the age-related neurodegeneration caused by loss of presenilin or γ-secretase and suggest that there is no detectable expression of Notch1 and Notch2 in pyramidal neurons of the adult cerebral cortex. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
36. Notch controls the magnitude of T helper cell responses by promoting cellular longevity.
- Author
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Helbig, Christina, Gentek, Rebecca, Flavell, Richard A., de Souza, Yevan, Derks, Ingrid A. M., Eldering, Eric, Wagner, Koen, Jankovic, Dragana, Gridley, Thomas, Moerland, Perry D., Flavel, Richard A., and Amsen, Derk
- Subjects
- *
T helper cells , *IMMUNOREGULATION , *CD4 antigen , *CELL proliferation , *IMMUNOGLOBULIN structure , *T cell receptors , *APOPTOSIS , *GENE expression - Abstract
Generation of effective immune responses requires expansion of rare antigen-specific CD4+ T cells. The magnitude of the responding population is ultimately determined by proliferation and survival. Both processes are tightly controlled to limit responses to innocuous antigens. Sustained expansion occurs only when innate immune sensors are activated by microbial stimuli or by adjuvants, which has important implications for vaccination. The molecular identity of the signals controlling sustained T-cell responses is not fully clear. Here, we describe a prominent role for the Notch pathway in this process. Coactivation of Notch allows accumulation of far greater numbers of activated CD4+ T cells than stimulation via T-cell receptor and classic costimulation alone. Notch does not overtly affect cell cycle entry or progression of CD4+ T cells. Instead, Notch protects activated CD4+ T cells against apoptosis after an initial phase of clonal expansion. Notch induces a broad antiapoptotic gene expression program that protects against intrinsic, as well as extrinsic, apoptosis pathways. Both Notch 1 and Notch2 receptors and the canonical effector RBPJ (recombination signal binding protein for immunoglobulin kappa J region) are involved in this process. Correspondingly, CD4+ T-cell responses to immunization with protein antigen are strongly reduced in mice lacking these components of the Notch pathway. Our findings, therefore, show that Notch controls the magnitude of CD4+ T-cell responses by promoting cellular longevity. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
37. Multiple functions of Snail family genes during palate development in mice.
- Author
-
Murray, Stephen A., Oram, Kathleen F., and Gridley, Thomas
- Subjects
- *
GENES , *CELLS , *GENE expression , *PALATE , *TRANSCRIPTION factors , *GENETIC transcription - Abstract
Palate development requires precise regulation of gene expression changes, morphogenetic movements and alterations in cell physiology. Defects in any of these processes can result in cleft palate, a common human birth defect. The Snail gene family encodes transcriptional repressors that play essential roles in the growth and patterning of vertebrate embryos. Here we report the functions of Snail (Snai1) and Slug (Snai2) genes during palate development in mice. Snai2-/- mice exhibit cleft palate, which is completely penetrant on a Snai1 heterozygous genetic background. Cleft palate in Snai1+/- Snai2-/- embryos is due to a failure of the elevated palatal shelves to fuse. Furthermore, while tissue-specific deletion of the Snai1 gene in neural crest cells does not cause any obvious defects, neural-crest-specific Snai1 deletion on a Snai2-/- genetic background results in multiple craniofacial defects, including a cleft palate phenotype distinct from that observed in Snai1+/- Snai2-/- embryos. In embryos with neural-crest-specific Snai1 deletion on a Snai2-/- background, palatal clefting results from a failure of Meckel's cartilage to extend the mandible and thereby allow the palatal shelves to elevate, defects similar to those seen in the Pierre Robin Sequence in humans. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
38. Notch2 governs the rate of generation of mouse long- and short-term repopulating stem cells.
- Author
-
Varnum-Finney, Barbara, Halasz, Lia M., Mingyi Sun, Gridley, Thomas, Radtke, Freddy, Bernstein, Irwin D., and Sun, Mingyi
- Subjects
- *
STEM cell research , *NOTCH proteins , *CELL proliferation , *BONE marrow cells , *LABORATORY mice ,HEMATOPOIETIC stem cell development - Abstract
HSCs either self-renew or differentiate to give rise to multipotent cells whose progeny provide blood cell precursors. However, surprisingly little is known about the factors that regulate this choice of self-renewal versus differentiation. One candidate is the Notch signaling pathway, with ex vivo studies suggesting that Notch regulates HSC differentiation, although a functional role for Notch in HSC self-renewal in vivo remains controversial. Here, we have shown that Notch2, and not Notch1, inhibits myeloid differentiation and enhances generation of primitive Sca-1(+)c-kit(+) progenitors following in vitro culture of enriched HSCs with purified Notch ligands. In mice, Notch2 enhanced the rate of formation of short-term repopulating multipotential progenitor cells (MPPs) as well as long-term repopulating HSCs, while delaying myeloid differentiation in BM following injury. However, consistent with previous reports, once homeostasis was achieved, neither Notch1 nor Notch2 affected repopulating cell self-renewal. These data indicate a Notch2-dependent role in assuring orderly repopulation by HSCs, MPPs, myeloid cells, and lymphoid cells during BM regeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
39. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis.
- Author
-
Xu, Keli, Nieuwenhuis, Erica, Cohen, Brenda L., Wang, Wei, Canty, Angelo J., Danska, Jayne S., Coultas, Leigh, Rossant, Janet, Wu, Megan Y. J., Piscione, Tino D., Nagy, Andras, Gossler, Achim, Hicks, Geoff G., Hui, Chi-Chung, Henkelman, R. Mark, Yu, Lisa X., Sled, John G., Gridley, Thomas, and Egan, Sean E.
- Subjects
- *
LUNG physiology , *NOTCH proteins , *PULMONARY endothelium , *GLUCOSAMINE , *TRANSFERASES , *MYOFIBROBLASTS , *EMBRYOLOGY - Abstract
Distal lung development occurs through coordinated induction of myofibmblasts, epithelial cells, and capillaries. Lunatic Fringe (Lfrzg) is a β1-3 N-acetylglucosamine transferase that modifies Notch receptors to facilitate their activation by Delta-like (Dll1/4) ligands. Lfng is expressed in the distal lung during saccular development, and deletion of this gene impairs myofibroblast differentiation and alveogenesis in this context. A similar defect was observed in Notch2β-geo/+Notch3β-geo/β-geo compound mutant mice but not in Notch2β-geo/+ single mutants. Finally, to directly test for the role of Notch signaling in myofibroblast differentiation in vivo, we used ROSA26-rtTA/+;tetO-CRE/+; RBPJκflox/flox inducible mutant mice to show that disruption of canonical Notch signaling during late embryonic development prevents induction of smooth muscle actin in mesenchymal cells of the distal lung. In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
40. Jagged1 is the pathological link between Wnt and Notch pathways in colorectal cancer.
- Author
-
Rodilla, Veronica, Villanueva, Alberto, Obrador-Hevia, Antonia, Robert-Moreno, Alex, Fernández-Majada, Vanessa, Grilli, Andrea, Lopez-Bigas, Nuria, Bellora, Nicolàs, Albà, M. Mar, Torres, Ferran, Duñach, Mireia, Sanjuan, Xavier, Gonzalez, Sara, Gridley, Thomas, Capella, Gabriel, Bigas, Anna, and Lluís Espinosa
- Subjects
- *
COLON cancer , *NOTCH genes , *WNT genes , *CARCINOGENESIS , *CELL proliferation , *LABORATORY mice - Abstract
Notch has been linked to β-catenin-dependent tumorigenesis; however, the mechanisms leading to Notch activation and the contribution of the Notch pathway to colorectal cancer is not yet understood. By microarray analysis, we have identified a group of genes downstream of Wnt/β-catenin (down-regulated when blocking Wnt/βcatenin) that are directly regulated by Notch (repressed by γ-secretase inhibitors and up-regulated by active Notchi in the absence of β-catenin signaling). We demonstrate that Notch is downstream of Wnt in colorectal cancer cells through β-catenin-mediated transcriptional activation of the Notch-ligand Jaggedi. Consistently, expression of activated Notchi partially reverts the effects of blocking Wnt/β-catenin pathway in tumors implanted s.c. in nude mice. Crossing APC[supMinl]with Jagged1[sup+/Δ] miceis sufficient to significantly reduce the size of the polyps arising in the APC mutant background indicating that Notch is an essential modulator of tumorigenesis induced by nuclear β-catenin. We show that this mechanism is operating in human tumors from Familial Adenomatous Polyposis patients. We conclude that Notch activation, accomplished by β-catenin-mediated up-regulation of Jagged1, is required for tumorigenesis in the intestine. The Notch-specific genetic signature is sufficient to block differentiation and promote vasculogenesis in tumors whereas proliferation depends on both pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
41. Interaction between Reelin and Notch Signaling Regulates Neuronal Migration in the Cerebral Cortex
- Author
-
Hashimoto-Torii, Kazue, Torii, Masaaki, Sarkisian, Matthew R., Bartley, Christopher M., Shen, Jie, Radtke, Freddy, Gridley, Thomas, Šestan, Nenad, and Rakic, Pasko
- Subjects
- *
CELL migration , *NEURONS , *CEREBRAL cortex , *NEOCORTEX - Abstract
Summary: Neuronal migration is a fundamental component of brain development whose failure is associated with various neurological and psychiatric disorders. Reelin is essential for the stereotypical inside-out sequential lamination of the neocortex, but the molecular mechanisms of its action still remain unclear. Here we show that regulation of Notch activity plays an important part in Reelin-signal-dependent neuronal migration. We found that Reelin-deficient mice have reduced levels of the cleaved form of Notch intracellular domain (Notch ICD) and that loss of Notch signaling in migrating neurons results in migration and morphology defects. Further, overexpression of Notch ICD mitigates the laminar and morphological abnormalities of migrating neurons in Reeler. Finally, our in vitro biochemical studies show that Reelin signaling inhibits Notch ICD degradation via Dab1. Together, our results indicate that neuronal migration in the developing cerebral cortex requires a Reelin-Notch interaction. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
42. Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1.
- Author
-
Robert-Moreno, Àlex, Guiu, Jordi, Ruiz-Herguido, Cristina, López, M. Eugenia, Inglés-Esteve, Julia, Riera, Lluis, Tipping, Alex, Enver, Tariq, Dzierzak, Elaine, Gridley, Thomas, Espinosa, Lluis, and Bigas, Anna
- Subjects
- *
HEMATOPOIESIS , *EMBRYOS , *NOTCH genes , *LIGANDS (Biochemistry) , *GENETIC transduction - Abstract
Specific deletion of Notch1 and RBPjκ in the mouse results in abrogation of definitive haematopoiesis concomitant with the loss of arterial identity at embryonic stage. As prior arterial determination is likely to be required for the generation of embryonic haematopoiesis, it is difficult to establish the specific haematopoietic role of Notch in these mutants. By analysing different Notch-ligand-null embryos, we now show that Jagged1 is not required for the establishment of the arterial fate but it is required for the correct execution of the definitive haematopoietic programme, including expression of GATA2 in the dorsal aorta. Moreover, successful haematopoietic rescue of the Jagged1-null AGM cells was obtained by culturing them with Jagged1-expressing stromal cells or by lentiviral-mediated transduction of the GATA2 gene. Taken together, our results indicate that Jagged1-mediated activation of Notch1 is responsible for regulating GATA2 expression in the AGM, which in turn is essential for definitive haematopoiesis in the mouse. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
43. Notch2 is required for maintaining sustentacular cell function in the adult mouse main olfactory epithelium
- Author
-
Rodriguez, Steve, Sickles, Heather M., DeLeonardis, Chris, Alcaraz, Ana, Gridley, Thomas, and Lin, David M.
- Subjects
- *
NEURONS , *NERVOUS system , *GLUTATHIONE , *ORGANS (Anatomy) - Abstract
Abstract: Notch receptors are expressed in neurons and glia in the adult nervous system, but why this expression persists is not well-understood. Here we examine the role of the Notch pathway in the postnatal mouse main olfactory system, and show evidence consistent with a model where Notch2 is required for maintaining sustentacular cell function. In the absence of Notch2, the laminar nature of these glial-like cells is disrupted. Hes1, Hey1, and Six1, which are downstream effectors of the Notch pathway, are down-regulated, and cytochrome P450 and Glutathione S-transferase (GST) expression by sustentacular cells is reduced. Functional levels of GST activity are also reduced. These disruptions are associated with increased olfactory sensory neuron degeneration. Surprisingly, expression of Notch3 is also down-regulated. This suggests the existence of a feedback loop where expression of Notch3 is initially independent of Notch2, but requires Notch2 for maintained expression. While the Notch pathway has previously been shown to be important for promoting gliogenesis during development, this is the first demonstration that the persistent expression of Notch receptors is required for maintaining glial function in adult. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
44. Direct Regulation of Gata3 Expression Determines the T Helper Differentiation Potential of Notch
- Author
-
Amsen, Derk, Antov, Andrey, Jankovic, Dragana, Sher, Alan, Radtke, Freddy, Souabni, Abdallah, Busslinger, Meinrad, McCright, Brent, Gridley, Thomas, and Flavell, Richard A.
- Subjects
- *
GENE expression , *T cells , *NOTCH genes , *CELL differentiation - Abstract
Summary: CD4+ T helper cells differentiate into T helper 1 (Th1) or Th2 effector lineages, which orchestrate immunity to different types of microbes. Both Th1 and Th2 differentiation can be induced by Notch, but what dictates which of these programs is activated in response to Notch is not known. By using T cell-specific gene ablation of the Notch effector RBP-J or the Notch1 and 2 receptors, we showed here that Notch was required on CD4+ T cells for physiological Th2 responses to parasite antigens. GATA-3 was necessary for Notch-induced Th2 differentiation, and we identified an upstream Gata3 promoter as a direct target for Notch signaling. Moreover, absence of GATA-3 turned Notch from a Th2 inducer into a powerful inducer of Th1 differentiation. Therefore, Gata3 is a critical element determining inductive Th2 differentiation and limiting Th1 differentiation by Notch. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
45. Mutation of a Ubiquitously Expressed Mouse Transmembrane Protein (Tapt1) Causes Specific Skeletal Homeotic Transformations.
- Author
-
Howell, Gareth R., Shindo, Mami, Murray, Stephen, Gridley, Thomas, Wilson, Lawriston A., and Schimenti, John C.
- Subjects
- *
GENETIC mutation , *CHROMOSOMES , *PROTEINS , *LABORATORY mice , *MEDICAL genetics - Abstract
L5Jcs1 is a perinatal lethal mutation uncovered in a screen for ENU-induced mutations on mouse chromosome 5. L5Jcs1 homozygotes exhibit posterior-to-anterior transformations of the vertebral column midsection, similar to mice deficient for Hoxc8 and Hoxc9. Positional cloning efforts identified a mutation in a novel, evolutionarily conserved, and ubiquitously expressed gene dubbed Tapt1 (Transmembrane anterior posterior transformation 1). TAPT1 is predicted to contain several transmembrane domains, and part of the gene is orthologous to an unusual alternatively spliced human transcript encoding the cytomegalovirus gH receptor. We speculate that TAPT1 is a downstream effector of HOXC8 that may act by transducing or transmitting extracellular information required for axial skeletal patterning during development. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
46. Snail regulates p21WAF/CIP1 expression in cooperation with E2A and Twist
- Author
-
Takahashi, Eishi, Funato, Noriko, Higashihori, Norihisa, Hata, Yuiro, Gridley, Thomas, and Nakamura, Masataka
- Subjects
- *
EMBRYOLOGY , *DEVELOPMENTAL biology , *NERVOUS system , *TRANSCRIPTION factors - Abstract
Abstract: Snail, a zinc-finger transcriptional repressor, is essential for mesoderm and neural crest cell formation and epithelial–mesenchymal transition. The basic helix–loop–helix transcription factors E2A and Twist have been linked with Snail during embryonic development. In this study, we examined the role of Snail in cellular differentiation through regulation of p21WAF/CIP1 expression. A reporter assay with the p21 promoter demonstrated that Snail inhibited expression of p21 induced by E2A. Co-expression of Snail with Twist showed additive inhibitory effects. Deletion mutants of the p21 promoter revealed that sequences between −270 and −264, which formed a complex with unidentified nuclear factor(s), were critical for E2A and Snail function. The E2A-dependent expression of the endogenous p21 gene was also inhibited by Snail. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
47. Notch3 is required for arterial identity and maturation of vascular smooth muscle cells.
- Author
-
Domenga, Valérie, Fardoux, Peggy, Lacombe, Pierre, Monet, Marie, Maciazek, Jacqueline, Gridley, Thomas, Krebs, Luke T., Klonjkowski, Bernard, Berrou, Eliane, Mericskay, Matthias, Zhen Li, Tournier-Lasserve, Elisabeth, and Joutel, Anne
- Subjects
- *
ARTERIES , *MICE , *MUSCLE cells , *CELLS , *EXCITABLE membranes , *MYOGENESIS - Abstract
Formation of a fully functional artery proceeds through a multistep process. Here we show that Notch3 is required to generate functional arteries in mice by regulating arterial differentiation and maturation of vascular smooth muscle cells (vSMC). In adult Notch3-/- mice distal arteries exhibit structural defects and arterial myogenic responses are defective. The postnatal maturation stage of vSMC is deficient in Notch3-/- mice. We further show that Notch3 is required for arterial specification of vSMC but not of endothelial cells. Our data reveal Notch3 to be the first cell-autonomous regulator of arterial differentiation and maturation of vSMC. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
48. γ-Secretase Functions through Notch Signaling to Maintain Skin Appendages but Is Not Required for Their Patterning or Initial Morphogenesis
- Author
-
Pan, Yonghua, Lin, Meei-Hua, Tian, Xiaolin, Cheng, Hui-Teng, Gridley, Thomas, Shen, Jie, and Kopan, Raphael
- Subjects
- *
SKIN , *HAIR follicles , *CYSTS (Pathology) , *SEBACEOUS glands , *PROTEOLYSIS - Abstract
The role of Notch signaling during skin development was analyzed using Msx2-Cre to create mosaic loss-of-function alleles with precise temporal and spatial resolution. We find that γ-secretase is not involved in skin patterning or cell fate acquisition within the hair follicle. In its absence, however, inner root sheath cells fail to maintain their fates and by the end of the first growth phase, the epidermal differentiation program is activated in outer root sheath cells. This results in complete conversion of hair follicles to epidermal cysts that bears a striking resemblance to Nevus Comedonicus. Sebaceous glands also fail to form in γ-secretase-deficient mice. Importantly, mice with compound loss of Notch genes in their skin phenocopy loss of γ-secretase in all three lineages, demonstrating that Notch proteolysis accounts for the major signaling function of this enzyme in this organ and that both autonomous and nonautonomous Notch-dependent signals are involved. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
49. Haploinsufficient lethality and formation of arteriovenous malformations in Notch pathway mutants.
- Author
-
Krebs, Luke T., Shutter, John R., Tanigaki, Kenji, Honjo, Tasuku, Stark, Kevin L., and Gridley, Thomas
- Subjects
- *
NOTCH genes , *VERTEBRATES , *LABORATORY mice , *EMBRYOS , *ENDOTHELIUM - Abstract
The Notch signaling pathway is essential for embryonic vascular development in vertebrates. Here we show that mouse embryos heterozygous for a targeted mutation in the gene encoding the DLL4 ligand exhibit haploinsufficient lethality because of defects in vascular remodeling. We also describe vascular defects in embryos homozygous for a mutation in the Rbpsuh gene, which encodes the primary transcriptional mediator of Notch signaling. Conditional inactivation of Rpbsuh function demonstrates that Notch activation is essential in the endothelial cell lineage. Notch pathway mutant embryos exhibit defects in arterial specification of nascent blood vessels and develop arteriovenous malformations. These results demonstrate that vascular remodeling in the mouse embryo is sensitive to Dll4 gene dosage and that Notch activation in endothelial cells is essential for embryonic vascular remodeling. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
50. Notch signaling regulates left-right asymmetry determination by inducing Nodal expression.
- Author
-
Krebs, Luke T., Iwai, Naomi, Nonaka, Shigenori, Welsh, Ian C., Yu Lan, Rulang Jiang, Saijoh, Yukio, O'Brien, Timothy P., Hamada, Hiroshi, and Gridley, Thomas
- Subjects
- *
NOTCH genes , *GENE expression , *CELLULAR signal transduction , *PHYSIOLOGY , *GENETIC regulation - Abstract
Investigates the role of Notch signaling pathway in the establishment of left-right asymmetry in mice. Regulation of Nodal gene expression; Physiology of the Notch signaling pathway; Presence of Nodal cilia in DII1 mutant embryos; Significance of Nodal gene expression to the determination of left-right axis.
- Published
- 2003
- Full Text
- View/download PDF
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