1. Effect of 4 hydroxy fatty acids on lipid accumulation in the 3T3-L1 cells: a comparative study.
- Author
-
Kaji N, Omae T, Matsuzaki H, and Yamamoto Y
- Subjects
- Animals, Mice, Cell Survival drug effects, Stearic Acids pharmacology, Fatty Acids metabolism, Oleic Acids pharmacology, 3T3-L1 Cells, Lipid Metabolism drug effects, Glycerolphosphate Dehydrogenase metabolism, Glycerolphosphate Dehydrogenase genetics, RNA, Messenger genetics, RNA, Messenger metabolism
- Abstract
Notwithstanding the several investigations of the hydroxy fatty acids (hFAs)' physiological functions, studies focusing on their anti-obesity effects are limited. This study investigated the anti-obesity effects of 4 hFAs-10-hydroxy stearic acid (10-hSA), 12-hydroxy stearic acid (12-hSA), 9,12-hydroxy stearic acid (9,12-dhSA), and 12-hydroxy oleic acid (12-hOA)-on the 3T3-L1 cells. All hFAs suppressed lipid accumulation, with 10-hSA and 12-hOA exhibiting the strongest suppression, followed by 12-hSA and 9,12-hSA. This trend was similar to that observed for the glycerol-3-phosphate dehydrogenase (GPDH) activity level. Contrastingly, only 9,12-dhSA suppressed cell viability. The mRNA levels of HK1 and Aldoa were markedly suppressed by 10-hSA and 12-hSA compared to the control. Additionally, mRNA expression of Gyk was considerably suppressed by 12-hSA. Thus, all hFAs suppressed lipid accumulation by suppressing GPDH activity, although their molecular mechanisms were different. These findings will aid the application of hFAs in the food and medical industries., (© The Author(s) 2024. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
- Published
- 2024
- Full Text
- View/download PDF