Search

Background: Traditionally, Portulaca oleracea Linn. is used for treating abscesses, dysentery and liver diseases. In addition, recent studies have reported its efficacy as an analgesic, as neuroprotective, anti-inflammatory, bronchodilatory, and anticancer agent, besides antioxidant, wound healing and other important pharmacological actions. Aim and Objective: For curing liver diseases, Chinese use juice from the fresh leaves of Portulaca oleracea Linn. Hence, to prove this claim, an in-vivo hepatoprotective study of extracts from Portulaca oleracea Linn. against carbon tetrachloride hepatotoxic rats was carried out. Methods: The in-vitro antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and hepatoprotective activity of extracts of Portulaca oleracea Linn. was assessed against the carbon tetrachloride-induced toxicity rat model. Results: The extracts of Portulaca oleracea Linn produced concentration-dependent percentage inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and most significantly, by methanol extract. Among the extracts, methanol extract showed a significant decrease in Serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) levels and a significant increase in total protein (TP) levels against carbon tetrachloride-induced liver toxicity Conclusion: From the results obtained, it was concluded that methanol extract of Portulaca oleracea Linn. possesses significant DPPH free radical scavenging and hepatoprotective potential.

BACKGROUND: Diarrhea is the particular disease that still affects children in Indonesia, with rotavirus being the most common etiology among children under 5 years old. Rotavirus and non-rotavirus diarrhea can spread to the extraintestinal and localized to the liver which causes liver cell damage, thus, the level of the glutamic oxaloacetic and glutamic pyruvic transaminase enzymes increases. AIM: The objective of the study was to prove that there are differences in serum levels of glutamic oxaloacetic and glutamic pyruvic transaminase in children with acute diarrhea due to rotavirus and non-rotavirus infection. METHODS: This study used a cross-sectional design, the research subjects were children aged 6 months old until 60 months old with acute diarrhea in Denpasar Public Health Center, Sanglah, and Wangaya General Hospital within the period of March 2018 until March 2021. Statistical analysis used the Mann–Whitney. RESULTS: A total of 70 subjects were analyzed in this study. There were 24.28% of subjects with rotavirus. Each group had nearly the same degree of severity of 29.4% for rotavirus and 30.2% for non-rotavirus, with a median of serum levels of glutamic oxaloacetic transaminase (SGOT) 47 (19–261) and glutamic pyruvic transaminase (SGPT) 25 (7–217). The results of this study showed that the median difference in aspartate aminotransferase and alanine aminotransferase levels was not significant in rotavirus and non-rotavirus diarrhea (SGOT 45 [16–168], 32 [11–261], p = 0.077; (SGPT 22 [14–91], 18 [5–217], p = 0.12). CONCLUSION: This study concluded that there is a higher median level of SGOT and SGPT in children with acute diarrhea due to rotavirus infection compared to non-rotavirus infection, although it is not statistically significant.

The current study was designed for two groups, each group contain (20) nonsmokers healthy persons males, first group was get (more than 7 hours sleep / day) , while the second group did not get enough sleep hours (less than 7 hours sleep / day). The results showed a significant increase in the level of body mass index (BMI) , the activity of glutamic pyruvic transaminase enzyme (GPT), glutamic oxaloacetic transaminase enzyme (GOT) and concentration of total serum bilirubin (TSB) at probability level (P≤0.049 , P≤0.047 , P≤0.032 , P≤0.035) Respectively in Persons with second group compared to Persons with first group, as well as the study showed a positive correlation between the studied parameters , with increase of all parameters in the second group with each other as a result of the same reason.

The menopause correlate with changes in physiological, psychological and biochemical characterized, and also body mass index. The study was analyzed the level of glutamic pyruvic transaminase activity (GPT) , glutamic oxaloacetic transaminase activity (GOT) , alkaline phosphatase activity (ALP) and estimation of body mass index (BMI) in postmenopausal women (n=20) (50-70)years old and premenopausal women (n=20) (30-40) years old. In postmenopausal women the activity of glutamic pyruvic transaminase (GPT) , glutamic oxaloacetic transaminase activity (GOT) , alkaline phosphatase activity (ALP) and body mass index (BMI) was elevated significantly as compared to premenopausal women (p

Objectives: To identify the phytoconstituents and determine the effect of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats. Methods: Phytochemical screening was performed using the drop Test. For hepatoxicity study, albino rats were randomized into 5 groups of 6 each. Healthy group: without hepatoxicity; Control group: hepatotoxicity; Curative group: hepatotoxicity plus fluid extract of N. albopunctatissimum Lellinger; Standard group: hepatotoxicity plus silymarin; Preventive group: Fluid extract of N. albopunctatissimum Lellinger for one week, then hepatotoxicity. Hepatotoxicity was induced with 56% (w / v) ethanol at doses of 7.6 mL / kg p.c every 12 hours for 7 days. Glutamic pyruvic transaminase (GPT) values were determined at baseline, 7 days after induction and 14 days after. A histopathological study of the livers was performed. Results: Phytochemical screening revealed the presence of polyphenols, anthocyanidins, saponins, flavonoids and tannins. The preventive and standard groups showed a very significant decrease in serum GPT levels compared to control group (p

This experiment was conducted to evaluate the growth performance and some hemato-biochemical parameters of broilers fed different levels of Pakia biglobosa leaf extract (BPE). Two hundred and fifty (250) one day old (Ross 308) broiler chicks were randomly assigned to five treatments with five replicate consisting of ten (10) bird each in a completely randomized design. Treatment 1 contained 0% BPE, Treatment 2, 3, 4 and 5 contained 5ml, 10ml, 15ml and 20ml per liter of water respectively. Clean feed and water were offered ad libitum throughout the experiment which lasted for 7 weeks during which data on performance, hematology and serum biochemical traits were collected. Results revealed that BPE had a significant (p0.05) on the feed intake of the birds. There was no significant (p>0.05) difference in PCV, Hb, RBC, MCV, MCH and MCHC values among the treatment. However, WBC and its differentials were significantly (p

Objective: The objective of the study was to evaluate the subchronic toxicity of Sauropus androgynus L. Merr. leaves soup. Methods: Subchronic oral toxicity tests were carried out for 28 days in female Wistar rats using conventional methods. Thirty rats were divided into six groups, namely, one control group and three test groups with each extract being given at a dose of 500 mg/kg body weight (BW), 1000 mg/kg BW, and 3000 mg/kg BW, and one group satellite control and satellite test group with doses of 3000 mg/kg BW were carried out for 14 days after 28 days of treatment to see the effects of reversibility. All rat groups were observed for behavior, development of BW, serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), creatinine serum, ratio of liver and lung organs to BW, and histology of liver and lung. Results: The macroscopic observation of rat’s lung and rat weight ratio did not show a significant difference to the control group (p>0.05). In addition, the ratio of liver volume to BW was significantly different between the doses of 1000 mg/kg BW and 3000 mg/kg BW with satellite groups 3000 mg/kg BW (p0.05). However, creatinine serum had the highest increase at a dose of 500 mg/kg BW and a dose of 1000 mg/kg BW. Reversibility effects were not seen after 14 days of the past day given Sauropus androgynous soup for 28 days in female Wistar rats. Conclusion: The given of S. androgynous soup for 28 days for female Wistar rats did not show any specific toxicity effect so that its use was relatively safe for the consumption under 30 days. This study is expected to be the information source about the safety profile of S. androgynus leaves soup consumption.

Objective: To determine effect of citrullus lanatus juice on hemoglobin and Serum Glutamic Pyruvic Transaminase (SGPT) level. Study design and setting: It was an experimental study conducted on healthy rabbits for sixty days in the research department of pharmacy University of Karachi. Methodology: Current study was planned to evaluate effect of Citrullus lanatus juice on red blood cells, hemoglobin and liver enzyme. 60 days study was performed at 2 different doses i.e 3and 6ml/kg on rabbits. These rabbits were from either gender and were divided into 3 groups their weight ranges from 1200 to 1800 grams. Group A is control group (Normal saline 6ml/kg), Group B is treated group (3 ml/kg), Group C is treated group (6ml/kg). After taking mean of all values they are compared with control group. Significance of mean can be estimated by Tukes Post Hoc Test. P

This study aimed to analyse the antioxidant activity, total phenols, and flavonoids of kersen leaves (Muntingia calabura L.) extract (KLE) and determine the hepatoprotective activity of KLE in soft drink induced mice. Soft drinks induction causes liver injuries which increases the serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) levels. Mice were divided into five treatment groups (n=5) and were given Tween 80 (1%) (normal control), Tween 80 (1%) and 7 ml soft drinks (negative control), KLE (1000, 1500, and 2000 mg/kg), and 7 ml soft drinks per oral once daily for 4 weeks. Antioxidant activity was measured by 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenols and flavonoids were analyzed by Folin-ciocalteu colorimetric and aluminium chloride (AlCl3) method. The blood samples were collected for biochemical analysis. The result showed that the KLE antioxidant activity were 11.11+0.50 ppm. Total phenols and flavonoids detected in the KLE samples were 2.19+0.12 mgGAE/g and 2.43+0.24 mgQE/g respectively. Mice treated with KLE had a significantly lower (p

Eclipta Alba has been used in traditional and folklore medicine to treat Hyperlipidemia and hepatic disorders. The present study was aimed to investigate the Antihyperlipidemic and hepatoprotective potentials of Eclipta Alba in high-fat diet -induced Albino rats and to determine the underlying mechanism. A total of 30 adult albino rats of Wistar strain weighing 165–215 g were utilized. Animals were treated with high-fat diet for 8 weeks followed by post-treatment of E. Alba for 1 week, 2 weeks, and 3 weeks, respectively. After 12 h of fasting on the last day of the experiment, serum blood samples were collected in EDTA vials and processed for biochemical analysis. A significant decrease in levels of total cholesterol and triglycerides was noted on animals treated with E. alba compared to high-fat diet animals. Treatment of hypercholesterolemic rats with E. Alba showed a marked decrease of serum low-density lipoprotein (LDL) and very LDL cholesterol concentrations compared to the hypercholesterolemic rats. High-fat diet feeding worsened the levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase enzymes, whereas the same markers were significantly improved by supplementation with E. alba compared to the normal group. E. alba acts as an antihyperlipidemic agent in hyperlipidemic conditions and helps for better health.

A quick prediction method may help confirm the diagnosis of Kawasaki disease (KD), and reduce the risk of coronary artery lesions. The purpose of this study was to evaluate potential candidate diagnostic serum proteins in KD using isobaric tagging for relative and absolute quantification (iTRAQ) gel-free proteomics. Ninety two subjects, including 68 KD patients (1.6 ± 1.2 years, M/F 36/32) and 24 fever controls with evident respiratory tract infection (2.1 ± 1.2 years, M/F 13/11) were enrolled. Medical records were reviewed for demographic and laboratory data. The iTRAQ gel-free proteomics was used to screen serum proteins completely and compare the difference between two groups followed by specific validation with ELISA. The candidate proteins and conventional laboratory items were selected for the prediction model of KD diagnosis by support vector machine. Five selected candidate proteins, including protein S100-A8, protein S100-A9, protein S100-A12, neutrophil defensin 1, and alpha-1-acid glycoprotein 1 were identified for developing the prediction model of KD diagnosis. They were used to develop an efficient KD prediction model with an area under receiver operating characteristic (auROC) value of 0.92 (95% confidence interval: 0.84, 0.98). These protein biomarkers were significantly correlated with the conventional laboratory items as follows: C-reactive protein, glutamic pyruvic transaminase, white blood count, platelet, segment and hemoglobin. These conventional laboratory items were used to develop a prediction model of KD diagnosis with an auROC value of 0.88 (95% confidence interval: 0.80, 0.96). Our result demonstrated that the prediction model with combined five selected candidate protein levels may be a good diagnostic tool of KD. Further prediction model with combined six conventional laboratory data is also an acceptable alternative method for KD diagnosis.

In the treatment of tuberculosis (TB), the patient is generally directly given Anti Tuberculosis Drugs (Obat Anti Tuberculosis/OAT) without examining Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT) to see whether or not there is liver damage before treatment. Because of the side effects by OAT, it is important to know how the condition of the liver function of TB patients who consume OAT in Kendari City General Hospital (Rumah Sakit Umum Daerah/RSUD) Kota Kendari by looking at SGOT and SGPT levels in order to provide maximum treatment to TB patients. The method of this research was descriptive analysis with cross sectional approach. The sample is all patients had previously been diagnosed with TB by a doctor based on medical records by in RSUD Kota Kendari after 1-2 months OAT treatment without any sign of liver injury before. Examination of SGOT and SGPT levels was performed on all samples in the Laboratory of RSUD Kota Kendari using clinical chemistry analyzer. The results of this study showed that 20% patients with TB had elevated levels of SGPT and SGOT with average SGOT is 51 U/L and SGPT is 42.5. The Inference of this study that there is an increase in SGOT and SGPT levels in patients who consume OAT 1-2 months in RSUD Kota Kendari. Elevated levels of SGPT and SGOT due to Anti Tuberculosis (OAT) drugs did not occur in all patients and only increased in 3 people, but qualitatively the effect of administering anti-tuberculosis drugs significantly increased levels of SGPT and SGPT. Thus, monitoring of liver physiology remains recommended especially for elderly patients.

Resumen Objetivos Buscar puntos de corte de la glutamico-piruvica transaminasa (GPT) y de ADN del virus de hepatitis B (ADN-VHB) al diagnostico, en pacientes con infeccion cronica VHB antigeno e negativo (AgHBe(–)), que puedan ser predictores de la evolucion, pronostico y/o de la necesidad de terapia antiviral. Metodos Estudio observacional de cohortes retrospectivo de pacientes diagnosticados de infeccion cronica por VHB AgHBe(–) (2005-2012). Se investigo un punto de corte de GPT normal al diagnostico que predijera la alteracion de esta en la evolucion, de ADN-VHB basal que predijera la elevacion de este por encima de 2.000 UI/ml, y de GPT y ADN-VHB como predictores de la necesidad de tratamiento, mediante curvas ROC. Resultados Se incluyeron 126 pacientes (seguimiento: 42,1 ± 21,5 meses), de los cuales 93 tenian GPT normal al diagnostico. En el analisis de curvas ROC el punto de corte de ADN-VHB que mejor predijo la elevacion de este por encima de 2.000 UI/ml fue 900 UI/ml (sensibilidad: 90%; especificidad: 88%; VPP: 79%; VPN: 100%; precision diagnostica: 89%), y el que mejor predijo la alteracion de GPT normal al diagnostico posteriormente elevada fue 25 mU/ml (sensibilidad: 95,4%; especificidad: 81,6%; VPP: 67%; VPN: 96%; precision diagnostica: 80,6%). Los pacientes con GPT 26-40 mU/ml al diagnostico presentaron mas complicaciones o necesidad de tratamiento que aquellos con GPT ≤ 25 mU/ml (p Conclusion Los pacientes VHB AgHBe(–) con GPT

Bali cattle are known for having high adaptability to any environmental condition and have proven tosurvive in critical environments including landfills. The aim of this study was to determine the relationshipbetween Serum Glutamic Pyruvic Transaminase /Serum Glutamic Oxaloacetic Transaminase (SGPT/SGOT) levels and lead (Pb) level in blood of bali cattle reared in the landfill Denpasar. A total of 22Balicattle were used in this study was. The blood was drawn from the jugular vein and 10 ml blood werecollected using tubes containing EDTA 0.5% and 10 mL without EDTA tubes. Levels of SGPT and SGOTwere measured by using Auto analyzer (Refloton (R) plus) method, while the measurement of Pb in bloodplasma was conducted by using atomic absorption spectrophotometry (AAS) method. Result of the SGPTand SGOT measurement obtained were relatively varied with an average of SGPT = 29.96±5.67 U/L andSGOT = 78.82±17.62.U/L. Similarly, Pb measurement results vary widely between 4.004 to 10.216 withan average of 6.595±1.85 ppm. Correlation analysis showed that there was an association between SGOTwith Pb levels. However, no correlation was observed between the SGPT with Pblevels. These resultsindicate that the levels of lead may cause pathological tissue cells in organs other than liver. Its concludedthat heavy metalPb is found in the blood of bali cattle reared in Denpasar city landfill, and it may becorrelated with the level of the SGOT and may cause pathological tissue cells organs.

Background: Although prolonged (30years) coffee consumption has been associated with reduced or increased frequency of liver (SGOT, SGPT, GGT) enzymes and cholesterol levels, it is unclear whether the effect is from coffee or caffeine. Methods: A self-administered questionnaire ascertained lifestyle characteristics, including alcohol consumption, cigarette smoking, Diabetes mellitus and Dietary habits. As for drinking habit, examinees were first asked about their current drinking frequency (none, 1-2 times/ week, or almost daily) past or current smokers about the number of cigarettes smoked per day and the duration of smoking in years. As regards coffee, examinees were asked their usual daily intake in cups. Results: A total No of 200 cases were studied by dividing them into two group’s controls and cases. The results so obtain were compared with 50 healthy controls (not to consumed caffeine contained things that include nor coffee, tea, any type of cola’s). Statistical evaluation was carried out to confirm any deviation from the normal values. In men the mean serum SGOT, SGPT, GGT and cholesterol of cases is having higher level as compared to the mean value of controls. This increase is statistically highly significant (SGOT

Anabas testudineus (Bloch) was exposed to 0.71 mg/L and 1.42 mg/L (25 and 50% of LC50 value respectively) of naphthalene, a polycyclic aromatic hydrocarbon (PAH), for 21 days. Blood biochemical parameters and erythrocytic morphological alterations were assessed to describe the naphthalene toxicity. Biochemical analysis showed a significant increase in glutamic pyruvic transaminase, GPT (576.7 ± 11.79 and 608.9 ± 12.08 U/L, respectively) and alkaline phosphatase, ALP (12.9 ± 0.69 and 13.4 ± 0.64 U/L, respectively) activities under two doses compared with control. Protein and albumin (ALB) content in blood decreased significantly, in comparison with control value in the tune of 22.67 ± 1.04 and 23.97 ± 1.24 g/dl, respectively and 10.7 ± 0.79 and 11.1 ± 0.67 g/dl, respectively. Erythrocytes showed varied symptomatic morphological changes under naphthalene exposure, which included severe denaturation, swelling in cells, appearance of sickle and tear drop cells, and cellular vacuolation. In particularly, the changes were more prominent under higher naphthalene exposure. Following the results, it has been able to establish that GPT, ALP, protein and ALB, and the morphological manifestations of erythrocytes would be good tools of biomarker in monitoring toxicological paradigm, especially to naphthalene exposure in aquatic bodies.

Background: Aegle marmelos (L.) Correa is a widely found plant in India as well as in South Asia. For more than several centuries, it is being widely used for its medicinal properties. Objective: The objective of this study was to evaluate the biochemical changes in alloxan-induced diabetic rats treated with methanolic leaf extracts of A. marmelos. Materials and Methods: Six treatment groups, namely control, diseased, standard (glimepiride), low dose (100 mg/kg), medium dose (250 mg/kg), and high dose (500 mg/kg) of methanolic leaf extracts, were used in the study. The biochemical effects were evaluated by the determination of bodyweight, blood glucose, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), total proteins, serum albumin, serum creatinine, and alkaline phosphatase. Results: A significant increase in the bodyweight of the animals was observed in the high-dose treated animals (350.0 ± 6.15) when compared to the diseased group animals (241.0 ± 7.23). A significant decrease in the blood glucose, SGOT, and SGPT levels was observed in the high-dose treated animals (142.3 ± 20.52, 71.6 ± 4.8, and 24.5 ± 2.42) when compared to the diseased group animals (292.8 ± 29.34, 146.3 ± 11.12, and 74.5 ± 2.88), respectively. Similarly, total proteins, serum albumin, serum creatinine, and alkaline phosphatase levels of the high-dose treated animals were also significantly decreased (6.1 ± 0.26, 4.2 ± 0.22, 0.4 ± 0.18, and 109.2 ± 14.58) when compared to the diseased group animals (9.7 ± 0.27, 5.4 ± 0.26, 1.0 ± 0.22, and 257.2 ± 8.22), respectively. Conclusion: Through the biochemical changes, it is evident that the high dose of methanolic leaf extract of A. marmelos can be used in the treatment of diabetes and its complications.

EFFECT OF POMEGRANATE PEEL ON THE BLOOD LIPID PROFILE OF EXPERIMENTAL RATS This study was carried to investigate the effects of pomegranate peels(Punic granatumL), on the blood lipid level, some physiological parameters as liver function, kidney function, histological properties of Heart in hypercholestrmic. Rats were divided into 2 groups first group as negative group and the second group thypercholestrmic group which divided into 4 groups positive control, and 3 thypercholestrmic groups, fed on several diets containing 5%, 10%,15% of pomegranate peels for 28 days. Peel was cleaned from impurities and washed with tap water. Drying Oven air dry at 45 °C was used for 48 hours to dry the peel, then ground in a Multi Mill apparatus to passe through a 50 mesh sieve to obtain a fine peel powder . Bodyweight gain, feed intake and feed efficiency ratio were calculated at the end of experiment. Fasting blood sample were taken on day 28 for the determination of serum total cholesterol (TC) , serum triglycerides (TG) , HDL cholesterol,LDL cholesterol ,creatinine, serum urea, aspartate aminotransferase (AST) and glutamic pyruvic transaminase (GPT). The heart of all animals were carefully removed and slices from them were fixed in 85% alcohol for 24 hours, and then processed for paraffin embedding. Histological sections, 7 micron-thick, were cut and stained with heamatoxylin and eosin. They were then microscopically examined to evaluate the effects of the three differ concentration of formalin on these organs. It was concluded that treatment using pomegranate peel (Punic granatum L), reduced significantly LDL-c ,Serum triglycerides in hypercholestremic rats which fed different levels of pomegranate peel and the decrease was the best as the level of supplement peels increased. تأثير قشور الرمان علي مستوي دهون الدم لفئران التجارب *وفائي زکي عازر ميخائيل،* حسن محمد صبحي، ** سحر عبد العزيز خيري، ** ماجدة رمزي قسطندي، ** علا علي علي قسم الإحتياجات الغذائية والنمو- المعهد القومى للتغذية- القاهرة** *معهد البحوث الافريقية قسم الموارد الطبيعية ،جامعة القاهرة يهدف هذا البحث إلى إمکانية إستخدام بعض مخلفات الثمار مثل قشور الرمان لإعداد وجبات غنية بالألياف عن طريق إضافتها بنسبة 5% ،10%، 15 % من القشور. فى هذة التجربة تم تقسيم الفئران إلى خمسة مجموعات المجموعة الأولى هى الکنترول السالب والأربع مجموعات الأخرى فتم إصابتها بإرتفاع الکوليتسرول وذلک بتغذيتها على 1,5% کوليسترول لمدة أسبوعين وترکت إحداهما کمجموعة ضابطة موجبة والثلاث مجموعات الأخرى تم إضافة مسحوق قشور الرمان (بعد تنظيفها بالماء وتجفيفها على درجة حرارة 45م لمدة 48 ساعة وطحنها) بنسب 5%، 10%، 15% وتم تغذية الفئران على الوجبات لمدة 28 يوم .فى نهاية التجربة تم ذبح الفئران وقياس مستوى الدهون في الدم والجليسريدات الثلاثية (TG) والکوليسترول HDLC, ووظائف الکلى والکبد وإجراء الفحص الهستولوباثولوجى للقلب. أوضحت النتائج انخفاض وزن الفئران عند تغذيتهم على القشور ولوحظ أيضا أن الالياف الموجودة فى القشور ادت الى انخفاض مستوى کلا من الکوليسترول الکلى والجليسريدات الثلاثية. وقد وجد ان تناول النسب العالية من القشور تؤدى إلى نتائج أفضل . الکلمات الکاشفة :- قشور الرمان - الکوليسترول- تراي جليسريد- وظائف الکلى- وظائف الکبد

Background : Liver participates in various metabolic processes in human body. Exposures to toxins such as carbon tetrachloride (CCl4) results in hepatocyte destruction and release the cell contents. Enzymes such as serum glutamic pyruvic transaminase (SGPT) were used as a parameter to diagnose liver damage. Pepino (Solanum muricatum Aiton) contains antioxidants that protect liver from hepatoxicity. The aim of this experiment is to determine the effect of pepino in protecting the hepatocyte from hepatotoxic effect of CCl4. Methods : A total of 16 Wistar rats used as the subject were divided into Control and Therapeutic Group. The Control group was induced with CCl4 but was not given pepino juice. Meanwhile, the Therapeutic Group was given pepino juice for 10 days. For induction of hepatotoxicity, CCl4 10% was given at a dosage of 2.0 ml/kg intra-peritoneal. Each Wistar rat in Therapeutic Group was given 1.0 ml of 300 g/ml pepino juice via oral feeding. Spectrophotometer with adjusted wavelength of 340 nm was used to measure SGPT level of the Wistar rats and statistical T-test was used to analyze the data. Results : Wistar rats in Therapeutic group showed a larger decrease (6898.9 IU/L–79.1 IU/L) in SGPT level compared to the Control group (6469.8 IU/L–418.5 IU/L). The SGPT level of the Wistar rat in Therapeutic group reached normal baseline (50 IU/L–150 IU/L). The significance of the experiment was supported by the T-test, P-value

The aim of present study was to compare levels of serum zinc and haptoglobin between patients with chronic active hepatitis C and controls. Two groups including Azeri patients with chronic active hepatitis C (n = 274) and their healthy family members (n = 100) were studied. Serum zinc and haptoglobin were measured using colorimetric and nephelometric methods, respectively. Patients were 156 males (56.9 %) and 118 females (43.1 %) with the mean age of 40.15 ± 12.24 years. Controls were 56 males (56 %) and 44 females (44 %) with the mean age of 39.32 ± 4.40 (p = 0.87 for sex and 0.51 for age). Both the mean levels of serum zinc (72.04 ± 19.08 vs. 106.18 ± 25.52 μg/dL) and haptoglobin (0.72 ± 0.45 vs. 1.08 ± 0.34 g/L) were significantly lower in patients than in controls (p

Glutamic-pyruvic transaminase (GPT) is one of the most important enzymes in human liver and has a valuable clinical reference for the diagnosis of many liver diseases. Here a method for the determination of GPT activity based on transdeamination is presented. In this method, a three electrode setup was used for the cyclic voltammetric determination of the enzyme. With the electrochemical detection of reduced nicotinamide adenine dinucleotide (NADH) produced from transdeamination, the GPT activity was characterized under optimal circumstances. Firstly, to verify the response of the electrode to NADH, a series of NADH concentrations varying from 39 μM to 2.5 mM were calibrated with cyclic voltammetry (CV). A linear relationship between the NADH concentration and the peak current with R2 0.9999 was obtained. Then the concentration of α-ketoglutarate (α-KG) which can exert great influence on transdeamination was also optimized and the most sensitive response was achieved at the point of 0.75 mM α-KG. Finally, the GPT activity was determined using both the unmodified screen-printed carbon electrode (SPCE) and the electrode modified with CNTs. The results showed that the relationship between the GPT activity and the peak current of the CV curve was linear between 60 U L−1 and 300 U L−1 and the modified electrode exhibited a slightly better linear relationship than the unmodified electrode. This work proposes a new enzymatic reactive system based on transdeamination for the electrochemical detection of GPT activity and combines the electrochemical detection of NADH with the determination of GPT activity.

It is well known that Cu(II) can coordinate with L-alanine (Cu–Ala), which can be destroyed through the addition of glutamic-pyruvic transaminase (GPT) since GPT can effectively catalyze the conversion of L-alanine into keto-acetic acid. As a result, the free Cu(II) ion can combine with bovine serum albumin (BSA) and in turn quench the fluorescence of BSA. In this context, a simple and sensitive GPT activity detection via fluorescence quenching method has been developed. The fluorescence intensity of the system shows a linear relationship with the GPT concentration in the range of 5 and 400 U L−1 with a detection limit down to 3 U L−1 (S/N = 3). Avoiding any labels or complicated operations, this cost-effective and convenient method holds the potential for the rapid diagnosis of GPT-related diseases.

Background: Kasni ( Cichorium intybus L.) reported to play an important role in the effective management of serum liver enzymes SGPT & SGOT in various animal models and this study is extension to newly diagnosed patients of type 2 diabetes mellitus. Methods: Newly diagnosed 90 patients of Type2 DM, age 35-65years, of either sex were divided into 3 groups. In group I only Metformin sustained release once a day and in group II/III 6 grams crude seed powder or 50 ml decoction of crude seed powder was given twice a day for 90 days in combination with Metformin sustained release orally once in a day. Serum liver enzyme levels of SGPT & SGOT were measured at zero, 30 th , 60 th and 90 th day. Results: All the three groups showed a significant reduction in SGPT & SGOT across the four time periods. Post hoc Tukey HSD test shown that there was a significant difference between group I & II (p=0.011) and group I & III (p=0.000) for SGPT and group I & II (p=0.012) and group I & III (p=0.000) for SGOT. Conclusions: The add on therapy with Kasni seed preparations is more effective for the management of altered SGPT and SGOT levels in Type2 diabetes mellitus patients than only oral hypoglycaemic agent in decreasing SGPT & SGOT of selected patients. Among Kasni seed preparation treated groups, decoction was found more effective than crude seed powder.

China is one of the leading nations in the use of medicinal plants, and medicinal plants play an outstanding role within the framework of the official health services in China. Modern research on Chinese medicinal plants included as a necessary prerequisite, the comprehensive understanding of their origin, distribution, resources, and therapeutic usage. In order to increase and promote the utilization of medicinal plants in various regions, it is necessary to train professionals. Medicinal plants and their preparations are controlled by the Chinese Pharmacopoiea and the Local Standard. Thus, Chinese medicinal plants have already made and will continue to make a great contribution to the health care of the Chinese people. The traditional Chinese drug has been commonly used as an astringent, but it was found clinically to exhibit therapeutic effects for certain forms of hepatitis, particularly in lowering the elevated serum glutamic pyruvic transaminase level.

The activity of glutamic pyruvic transaminase (GPT) is an important clinical evidence for some acute diseases such as acute hepatopathy and myocardial infarction. Thus, there is a demand for rapid determination of GPT in small formats at point-of-need. Herein, we describe a novel method of electrochemical determination of GPT with microfluidic technique. GPT activity was indirectly determined via the electrochemical (EC) detection of nicotinamide adenine dinucleotide (NADH) produced from the GPT transdeamination reaction. A type of microfluidic chip was developed, in which a passive mixer comprising 100 sub-ribs and a three-electrode strip for EC were integrated. To verify the response to NADH, a series of NADH concentrations varying from 19 µM to 5 mM were calibrated with cyclic voltammetry within the microfluidic chip. And a linear relationship with R 2 0.9982 between the peak current and the concentration of NADH was obtained. Then, the GPT activity was determined using the chips containing and not containing a ribs-type mixer. And a linear relationship which contained two sections between the GPT activity and the peak current was obtained. The chip with a ribs-type mixer exhibited the sensitivity of 0.0341 μA U−1 L in the range of 10–50 U L−1 and 0.0236 μA U−1 L in the range of 50–250 U L−1. And the detection limit of the chip with a ribs-type mixer was 9.25 U L−1. The complete detection process of GPT activity within the microfluidic chip was realized, and the time-consuming problem was remarkably improved too.

This study was done to evaluate possible hepatoprotective effects of aqueous leaf extracts of Basella alba in comparison with silymarin in paracetamol-induced hepatotoxicity in albino rats. Six groups of six albino rats each received orally for 6 weeks, vehicle, paracetamol (2 g/kg/day), paracetamol (2 g/kg/day) plus silymarin (50 mg/kg/day), paracetamol (2 g/kg/day) plus B. alba extract (60 mg/kg/day), paracetamol (2 g/kg/day) plus B. alba extract (80 mg/kg/day) and paracetamol (2 g/kg/day) plus B. alba extract (100 mg/kg/day). Hepatoprotective effect was evaluated by comparing serum bilirubin, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, proteins, alkaline phosphatase and liver histopathology. Results were represented as mean ± SEM. One-way ANOVA was done followed by post hoc Tukey's test with a highly significance level of P0.001. Aqueous leaf extracts of B. alba 100 mg/kg/day orally had significant hepatoprotective effect in paracetamol-induced hepatotoxicity in albino rats. The results were well comparable and even in some respects superior to standard drug silymarin.

The purpose of this study was to investigate the protective effects of fucoidan on acute alcohol-induced liver injury in rats. Experimental animals were randomly divided into four groups: (1) a control group, (2) a group fed 25% ethanol, (3) a group fed 25% ethanol and 250 mg/kg BW of fucoidan (25% ethanol+FUCO250), and (4) a group fed 25% ethanol and 500 mg/kg BW of fucoidan (25% ethanol+FUCO500). Each group was fed orally two times per day for 15 days. Liver weights in the 25% ethanol group were increased compared to the control group, while liver weights in the 25% ethanol+FUCO500 group were significantly decreased compared to the 25% ethanol group (p

This study measured activities of serum enzymes alkaline phosphatase, acid phosphatase, glutamic oxaloacetic transaminase (SGOT), and glutamic pyruvic transaminase (SGPT), markers of liver function in albino rats after continuous ingestion of arsenic trioxide (As203). For the study, treated animals were given AS2O3 (0.2 mg/100 g/day) orally for 180 days. After the completion of treatment, the blood was collected for the estimation of serum biochemical markers. The results obtained were compared with control group. Data showed a significant increase in SGOT and SGPT activity after 60 days of As203 administration. The level of alkaline phosphatase and acid phosphatase increased significantly after 90 and 120 days, respectively. Since the elevation of these serum enzymes is an indicator of hepatic damage, data indicate that As2O3 produces hepatotoxicity. When taken continuously, arsenic was also deposited in liver and blood and affected the enzymatic pathways. Total accumulated arsenic was determined by HG-A...

The present investigation was aimed to examine the safety parameters in the traditional use of Prunus persica (L) Batsch and Clerodendrum colebrookianum Walp as antihypertensive agents in northeast India. The subchronic toxicity of aqueous extracts of the leaves of C. colebrookianum (AECc) and P. persica (AEPp) at an oral dose 150 mg/kg bw/day was evaluated. The behavioral studies were carried out for locomotors activity (LMA) and muscle grip strength (MGS) activity in mice before and after two weeks of treatment. The biochemical estimation was carried out on the 14th day using blood and brain tissue samples of mice. One group of mice was used as control. In behavioral studies, 32.29% LMA and 39.38% MGS were decreased in AEPp-treated animals, whereas in AECc-treated animals 4.97% LMA and 24.57% MGS were increased on the 14th day using actophotometer and rotarod apparatus, respectively. The biochemical serum activities of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase as we...

Background: Inhalant (or solvent) abuse is the purposeful inhalation of vapors or gases, intended to produce pleasurable psychoactive effects. There is a dearth of Indian studies on inhalant users. Aim: The present study aimed to describe the socio-demographic, clinical, and psychosocial characteristics of inhalant users visiting a Tertiary Care Center in North India. Materials and Methods: The study was a retrospective chart review for 50 inhalant users who sought treatment for the first time from the center over a period of 2 years. All patients seeking treatment for inhalant use at the center were evaluated by a psychiatrist. Results: Mean age of the sample was 17.16±4.09 years and majority comprised of children and adolescents (72.2%). There were only three girls (6%). Majority comprised of school drop-outs (82%), from lower socio-economic status (80%). Mean age of initiation of first substance was 14.13±4.27 years and inhalants were first drugs for 38%. Duration of inhalant use ranged between 1 month and 7.5 years. Use was mostly uninterrupted, and 88% were dependent users. Correction fluid was the commonest product, used by huffing or sniffing. A large majority (86%) had used at least one other substance besides inhalants, and 8% reported involvement in high-risk sexual behaviors. Comorbid psychiatric disorder was seen in 8% of sample. Positive family history was observed in 30% of the sample. The mean hemoglobin of the sample was 11.88±0.60, with low hemoglobin in 25% of users. Neutrophils, lymphocytes, eosinophils, and monocytes were elevated beyond normal in 10.8%, 6.5%, 15.2%, and 7.5%, respectively. There was no evidence of leucopenia. Bilirubin and serum glutamic pyruvic transaminase was elevated in 6.6% and 13% of inhalant users, respectively. Conclusion: The study adds to the limited date available on the treatment-seeking inhalant users from Indian settings. There is a need to examine the pattern of inhalant use in larger samples, across multiple sites in a prospective manner.

This study was conducted to investigate the dietary effect of conventional or lutein-fortified chlorella on milk production and lutein incorporation in milk. Fifteen Holstein cows in mid-lactation were used in a 3 × 3 Latin square design each with a 21-day period. Cows were top-dressed daily with 30 g of conventional or lutein-fortified chlorella for 3 weeks. Cows without chlorella served as the control. The feed intake and milk yield were not affected by dietary treatments. The concentrations of milk protein and solids non-fat in groups fed diets containing both conventional and lutein-fortified chlorella were significantly higher than those of the control group (P

Purpose: to discuss the biologic and chemical mechanism of the influence on the organisms' health exerted by different athletic loads. Method: experimental method is adopted to observe the influence of different athletic loads for six weeks continuously on such indices as IgG, CK, LDH, GPT-ALT and so on in the rats' blood. Results: The IgG in the rats' serum of 15 normal exercise rats in this test significantly rises with significant difference compared with the control group (P

Background Childhood obesity is a major health concern. Obesity is due to an expansion of adipose tissue mass. This tissue produces pro􀁞inflarmnatory cytokines, such as interleukin􀁞6 (IL--6). IL􀁞6 is considered to be the chief stimulator of the production of highly sensitive C􀁞reactive protein (hsCRP) in the liver. Both molecules are responsible for the chronic low􀁞grade inflammatory state in obese individuals. Objective To assess a correlation between IL􀁞6 and hsCRP in obese adolescents. Methods This cross􀁞sectional study was conducted from March to June 2011 in Manado. Subjects were obese and normal body mass index (BMI) teens aged 13􀁞18 years. Serum glutamic oxaloacetic transaminase (SOOT) and serum glutamic pyruvic transaminase (SOPT) levels were measured to rule out liver impairment. IL􀁞6 and hsCRP levels were also measured. Data was analyzed by Pearson's correlation and linear regression to test for correlation between IL--6 and hsCRP levels. Results There was a strongly positive correlation between IL􀁞6 and hsCRP levels in obese adolescents (r=0.79 with P

Objective To study the hepatoprotective activity of methanol and aqueous extracts of Amorphophallus paeoniifolius tubers against paracetamol induced liver damage in rats. Methods Hepatotoxicity was induced by paracetamol and the biochemical parameters such as serum glutamic pyruvic transaminase (sGPT), serum glutamic oxaloacetic transaminase (sGOT) and serum alkaline phosphatase (sALP), serum bilirubin (SB) and histopathological changes in liver were studied along with silymarin and Liv-52 as standard hepatoprotective agents. Results The phytochemical investigation of the extracts showed presence of carbohydrates, proteins, steroids and flavonoids. Pre-treatment of the rats with methanol and aqueous extract prior to paracetamol administration caused a significant reduction in the values of sGOT, sGPT, sALP and sB ( P Conclusions From the results it can be concluded that Amorphophallus paeoniifolius possesses hepatoprotective effect against paracetamol-induced liver damage in rats.

This study was designed to investigate the ability of Lactobacillus plantarum as hepatoprotective microorganism against Salmonella typhimurium in mice. Twelve albino mice were divided into 4 groups designated as CC, PC, SC and PS. Each group consisted of 3 mice, and was subjected to the following treatments; CC; this group was used as a control. PC; this group was dosed with 0.1ml of 10 9 cfu/ml Lb plantarum culture.SC; this group was dosed with 0.1ml. of 2.5×10 7 cfu/ml Sal. typhimurium culture. PS; this group was dosed with 0.1ml of 10 9 cfu/ml Lb. plantarum culture, and infected with 0.1ml of 2.5 ×10 7 cfu/ml culture of Sal. typhimurium, then serum levels of Glutamic Pyruvic Transaminase (GPT) and Glutamic Oxaloacetic Transaminase (GOT) of treated mice were measured and histological sections were made from liver to evaluate protective effect of Lb. plantarum . Results showed that mice treated with Lb plantarum exhibited a significant (p≤ 0.05) decrease in serum level of GPT and GOT (43.25 U/L and 95.50 U/L, respectively) in comparison with their levels in serum of control group. Mice infected with Sal. typhimurium showed a significant (p≤ 0.05) increase in serum level of GPT (87.3 U/L), and a significant increase (p≤0.05) in serum level of GOT (227 U/L) in comparison with their levels (55.23 U/L and 113 U/L) in control group. Mice treated with Lb. plantarum and infected with Sal. typhimurium showed a significant (p≤ 0.05) decrease in serum GPT level (47.5U/L) and significant decrease (P≤0.05) in serum GOT level (121 U/L) in comparison with their levels in mice infected with Sa l. typhimurium. Histopathological study showed that infection with Sal. typhimurium caused a necrosis, degenerative changes and inflammatory cells infiltration as compared with control while, treatment with Lb plantarum prevented the histopathological effe ct of Sal. typhimurium on mice liver.

Background: Administration of high dose paracetamol may increase the metabolic pathways that produce N-acetyl-p-benzoquinonimin (NAPQI). NAPQI is a reactive substance, resulting liver tissue damage and increase SGPT activity. Red algae extract contains flavonoids that act as antioxidants. Objectives: This research aimed to determine the effect of red algae ( Gracilaria verrucosa ) extract to the activity of serum glutamic pyruvic transaminase (SGPT) in male Wistar rats ( Rattus norvegicus ) induced by high dose paracetamol. Methodology: This research used 24 animal of Male Wistar rats. These rats were divided into 3 groups: 1) group of rats fed with standard food; 2) group of rats given paracetamol 1750 mg/kg on day 10; and 3) group of rats given red algae extract 200 mg/kg/day on day 1-11 and paracetamol 1750mg/kg on day 10. On day 11, all rats were sacrificed and SGPT activities were measured by spectrophotometry method. Result: Mann-Whitney test results showed significant difference (p = 0.021) between SGPT activity of group of rats fed with standard food (=81.95±10.103 U/L) and group of rats induced by high dose paracetamol (=97.06±11.19 U/L). Furthermore, there was significant difference (p = 0.016) between SGPT activity of group of rats induced by high dose paracetamol (=97.06±11.19 U/L) and group of rats induced by high doses of paracetamol and fed with red algae extract (=82.73±11.233 U/L). Conclusion: The conclusion of this research showed that high dose paracetamol significantly increased SGPT activity and red algae extract significantly decreased SGPT activity because it contains flavonoids that act as antioxidants.

Zusammenfassung Der Einflus eines Kalteschocks auf GOT- und GPT-Freisetzung von Bullen- und Widder-Spermatozoen In einer experimentellen Arbeit wird die Freisetzung von GOT und GPT bei Bullen-und Widder-Spermatozoen durch einen Kalteschock untersucht. Hierzu wurden Ejakulate bei beiden Tierarten durch artifizielle Vagina bzw. Elektrostimulation gewonnen. Von den Bullen wurden die Samenproben 1:10 mit Natrium-Zitrat-Puffer und TrisZitronensaure-Fructose-Losung verdunnt; die Proben von den Widdern wurden ebenfalls 1:10 verdunnt allerdings nur mit Natrium-Zitrat-Puffer. Die Ejakulate wurden einem Kalteschock ausgesetzt (10 Min. auf 0° C) und anschliesend wurden GOT und GPT in der Extracellularflussigkeit bestimmt. Dabei ergaben sich hochsignifikante Unterschiede (p < 0,01) in der Freisetzung von GOT bei Bullen- und Widder-Spermatozoen, die in Natriumzitratlosung verdunnt waren. Die GOT-Freisetzung in Tris enthaltendem Puffer war dagegen nicht signifikant. Die Konzentration von GPT in dem Etrazellular-medium von Widdersperma war hoher als im Bullensamen. Eine signifikante Differenz (p< 0,05) wurde in der Freisetzung von GPT von Bullen-Spermatozoen gefunden, die in Zitratpuffer verdunnt waren, nicht dagegen in Tris. Fur Widdersperma wurde keine signifikante GPT-Freisetzung nachgewiesen. Resumen Efecto del choque frio sobre la liberacion de GOT y GPT de los espermatozoides del toro y del camera Se obtuvieron semenes de toro y carnero mediante vagina artificial y estimulacion electrica respectivamente. Las muestras de semen de toro fueron diluidas (al 1:10) con citrato sodico y solution tris acido citrico y fructosa. Las muestras de carnero fueron diluidas (al 1:10) solo con citrato sodico. Los semenes fueron expuestos a un “choque frio” de 0°C durante 10 minutos, y se determino la liberacion de GOT y GPT en el medio extracelular. Se aprecio una diferencia altamente significativa (p < 0,01) en la liberacion de GOT de los espermatozoides de toro y carnero diluidos con tampon de citrato sodico. La liberacion de GOT en tamon Tris no fue significativa. La concentration de GPT en el medio extracelular de semen de carnero, fue mas alta que en el de semen de toro. Se encontro una diferencia significativa (p < 0,05) en la liberacion de GPT de espermatozoides de toro diluidos con tampon citrato, pero no con Tris. Para el semen de carnero, la liberacion de GPT de los espermatozoides no fue significativa.

Twenty-eight healthy female Kilis goats were used to investigate the changes which occur in certain hematological and biochemical plasma parameters during the peri-partum period. The hematological parameters recorded were Ca, P, total protein, total cholesterol, Glutamic oxalacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT). A marked decrease ( p 6 /mm 3 ) was recorded from early gestation to 16.94 ± 0.23 and 15.40 ± 0.49 and 20.94 ± 1.52 and 16.50 ± 0.54 during mid-pregnancy, late pregnancy, parturition and postpartum, respectively. In addition, no significant change in Hb concentration at 11 and 18 weeks of gestation and the 3rd week after parturition was recorded. The observed leucocytosis postpartum was accompained with lymphopenia and eosinopenia ( p p p p p p p p p

A total of semen samples were collected from Mithun bulls reared at the farm of National Research Centre on Mithun NRCM Nagaland to measure cholesterol mg dl total protein g dl glutamic oxaloacetic transaminase IU L glutamic pyruvic transaminase IU L lactic dehydrogenase IU L glucose mg dl sodium mmol L and potassium mmol L and their mean concentrations were recorded as plusmn plusmn plusmn plusmn plusmn plusmn plusmn and plusmn respectively

The aim of this study was to determine the efficacy of xylanase supplementation in the diet on Thai native chicken performance. 36 birds (12 birds /group) were divided into three groups. The first group was administered a control diet and the second and third group were fed an experimental diet with two different levels, 10g/kg and 30g/kg, respectively. Live weight, feed intake, survivability the primary toxicity effect on organ weights and plasma biochemistries were recorded and evaluated. The results indicate that xylanase supplementation improves Thai native chicken performance by increasing live weight and decreasing feed conversion efficiency (FCE) and has no effect on survivability. Xylanase supplementation lead to a decreased blood urea nitrogen (BUN) level in 30g/kg diet, but was a slightly increased serum glutamic pyruvic transaminase (SGPT) levels. In addition xylanase supplementation had no effect on the internal organs. Therefore, it can be concluded that xylanase supplementation efficiently can improve Thai native chicken.

BACKGROUND Use of liver grafts from non-heart-beating donors (NHBDs) warrants consideration so to expand the donor pool. Because the results of controlled NHBDs (CNHBDs) were acceptable, we have recently tried to expand the criteria to older CNHBDs. Here, we report our experience using liver grafts from older CNHBDs. METHODS We retrospectively studied our donor records from June 1994 through December 2001. CNHBDs were divided into two groups by age: older donors (O) were more than or equal to 55 years old, and younger donors (Y) were less than 55 years old. We compared donor and recipient demographics and peak laboratory values during the first postoperative week. RESULTS Twenty-five grafts from CNHBDs were transplanted in our center. Five livers were harvested from O (63+/-6 years) and 20 were from Y (32+/-15 years). No differences other than age in donor characteristics were noted between O and Y. Mean age of recipients was 50 years in both groups. Mean cold ischemic time (CIT) was 5.4 hours in O and 7.3 hours in Y (P

Objetivo: Analisar os marcadores hepáticos (transaminases) em pacientes com malária causada pelo Plasmodium vivax. Métodos: Trata-se de um estudo transversal retrospectivo, incluindo pacientes que apresentaram monoinfecção pelo P. vivax. O teste de Mann-Whitney U ou o teste t de Student foram utilizados para comparar a atividade média das transaminases de acordo com a classificação clínica e epidemiológica do paciente infectado. O teste de Kruskal-Wallis foi utilizado para comparar a média da atividade das transaminases de acordo com a classificação da infecção. Valores de p

Background: In recent years, occupational health hazards have grown as one of the major public health issues worldwide. In this context, petrol pump workers were studied who by virtue of their occupation is continuously exposed to the various chemical substances and volatile organic compounds liberated in the form of gasoline vapors in their breathing zone. Aims and Objectives: To explore the effects of gasoline vapors on some biochemical parameters (the liver enzymes) among the petrol pump filling attendants working at various petrol pumps of Jaipur city. Materials and Methods: This study was conducted with 80 subjects (40 petrol pump workers working for more than three years at various petrol pumps of Jaipur city as study group and 40 matched male adults working as peons and ward boys in the different departments of the institute were considered as the control group). Liver enzymes were assessed using fully automated analyzer (Beckman Coulter AU680) available at the Central Biochemistry Laboratory in S.M.S Hospital Jaipur. Mean±standard deviation values for each parameter were determined for both the study and control groups and compared using an unpaired t–test, and P < 0.05 was considered significant. Results: Liver enzymes: serum glutamic pyruvic transaminase and serum glutamic oxaloacetic transaminase were found significantly higher among the petrol filling attendants in comparison to the control group, except serum alkaline phosphatase which was although higher in petrol filling attendants but statistically insignificant between the two groups. Conclusions: This study observed that the petrol pump workers are at greater risk of developing biochemical alterations in the hepatic enzymes with time. However, further longitudinal studies with larger sample size and regular follow-ups are required to get better insights into the results.