Author: "François-Xavier Cantrelle" - Searchworks@Jio Institute Digital Library Search Results

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1. Dynamic interactions and Ca2+-binding modulate the holdase-type chaperone activity of S100B preventing tau aggregation and seeding

Author: Guilherme G. Moreira, François-Xavier Cantrelle, Andrea Quezada, Filipa S. Carvalho, Joana S. Cristóvão, Urmi Sengupta, Nicha Puangmalai, Ana P. Carapeto, Mário S. Rodrigues, Isabel Cardoso, Güenter Fritz, Federico Herrera, Rakez Kayed, Isabelle Landrieu, and Cláudio M. Gomes
Subjects: Science
Abstract: The calcium binding protein S100B is an abundantly expressed protein in the brain and has neuro-protective functions by inhibiting Aβ aggregation and metal ion toxicity. Here, the authors combine cell biology and biochemical experiments with chemical kinetics and NMR measurements and show that S100B protein is an extracellular Tau chaperone and further characterize the interactions between S100B and Tau.
Published: 2021
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2. Phosphorylation and O-GlcNAcylation of the PHF-1 Epitope of Tau Protein Induce Local Conformational Changes of the C-Terminus and Modulate Tau Self-Assembly Into Fibrillar Aggregates

Author: François-Xavier Cantrelle, Anne Loyens, Xavier Trivelli, Oliver Reimann, Clément Despres, Neha S. Gandhi, Christian P. R. Hackenberger, Isabelle Landrieu, and Caroline Smet-Nocca
Subjects: Alzheimer’s disease, microtubule-associated protein tau, phosphorylation, O-GlcNAc glycosylation, protein aggregation, NMR spectroscopy, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract: Phosphorylation of the neuronal microtubule-associated Tau protein plays a critical role in the aggregation process leading to the formation of insoluble intraneuronal fibrils within Alzheimer’s disease (AD) brains. In recent years, other posttranslational modifications (PTMs) have been highlighted in the regulation of Tau (dys)functions. Among these PTMs, the O-β-linked N-acetylglucosaminylation (O-GlcNAcylation) modulates Tau phosphorylation and aggregation. We here focus on the role of the PHF-1 phospho-epitope of Tau C-terminal domain that is hyperphosphorylated in AD (at pS396/pS404) and encompasses S400 as the major O-GlcNAc site of Tau while two additional O-GlcNAc sites were found in the extreme C-terminus at S412 and S413. Using high resolution NMR spectroscopy, we showed that the O-GlcNAc glycosylation reduces phosphorylation of PHF-1 epitope by GSK3β alone or after priming by CDK2/cyclin A. Furthermore, investigations of the impact of PTMs on local conformation performed in small peptides highlight the role of S404 phosphorylation in inducing helical propensity in the region downstream pS404 that is exacerbated by other phosphorylations of PHF-1 epitope at S396 and S400, or O-GlcNAcylation of S400. Finally, the role of phosphorylation and O-GlcNAcylation of PHF-1 epitope was probed in in-vitro fibrillization assays in which O-GlcNAcylation slows down the rate of fibrillar assembly while GSK3β phosphorylation stimulates aggregation counteracting the effect of glycosylation.
Published: 2021
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3. Structural Basis of Tau Interaction With BIN1 and Regulation by Tau Phosphorylation

Author: Alessia Lasorsa, Idir Malki, François-Xavier Cantrelle, Hamida Merzougui, Emmanuelle Boll, Jean-Charles Lambert, and Isabelle Landrieu
Subjects: protein-protein interaction, nuclear magnetic resonance spectroscopy, Alzheimer’s disease, bridging integrator-1 BIN1, SH3 domain, tau, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract: Bridging integrator-1 (BIN1) gene is associated with an increased risk to develop Alzheimer’s disease, a tauopathy characterized by intra-neuronal accumulation of phosphorylated Tau protein as paired helical filaments. Direct interaction of BIN1 and Tau proteins was demonstrated to be mediated through BIN1 SH3 C-terminal domain and Tau (210–240) peptide within Tau proline-rich domain. We previously showed that BIN1 SH3 interaction with Tau is decreased by phosphorylation within Tau proline-rich domain, of at least T231. In addition, the BIN1/Tau interaction is characterized by a dynamic equilibrium between a closed and open conformations of BIN1 isoform 1, involving an intramolecular interaction with its C-terminal BIN1 SH3 domain. However, the role of the BIN1/Tau interaction, and its potential dysregulation in Alzheimer’s disease, is not yet fully understood. Here we showed that within Tau (210–240) peptide, among the two proline-rich motifs potentially recognized by SH3 domains, only motif P216TPPTR221 is bound by BIN1 SH3. A structural model of the complex between BIN1 SH3 and Tau peptide (213–229), based on nuclear magnetic resonance spectroscopy data, revealed the molecular detail of the interaction. P216 and P219 within the proline-rich motif were in direct contact with the aromatic F588 and W562 of the BIN1 SH3 domain. The contact surface is extended through electrostatic interactions between the positively charged R221 and K224 residues of Tau peptide and those negatively charged of BIN1 SH3, corresponding to E556 and E557. We next investigated the impact of multiple Tau phosphorylations within Tau (210–240) on its interaction with BIN1 isoform 1. Tau (210–240) phosphorylated at four different sites (T212, T217, T231, and S235), contrary to unphosphorylated Tau, was unable to compete with the intramolecular interaction of BIN1 SH3 domain with its CLAP domain. In accordance, the affinity of BIN1 SH3 for phosphorylated Tau (210–240) peptide was reduced, with a five-fold increase in the dissociation constant, from a Kd of 44 to 256 μM. This study highlights the complexity of the regulation of BIN1 isoform 1 with Tau. As abnormal phosphorylation of Tau is linked to the pathology development, this regulation by phosphorylation might have important functional consequences.
Published: 2018
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4. Direct Crosstalk Between O-GlcNAcylation and Phosphorylation of Tau Protein Investigated by NMR Spectroscopy

Author: Gwendoline Bourré, François-Xavier Cantrelle, Amina Kamah, Béatrice Chambraud, Isabelle Landrieu, and Caroline Smet-Nocca
Subjects: Tau protein, phosphorylation, O-GlcNAcylation, crosstalk, NMR spectroscopy, Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract: The formation of intraneuronal fibrillar inclusions of tau protein is associated with several neurodegenerative diseases referred to as tauopathies including Alzheimer's disease (AD). A common feature of these pathologies is hyperphosphorylation of tau, the main component of fibrillar assemblies such as Paired Helical Filaments (PHFs). O-β-linked N-acetylglucosaminylation (O-GlcNAcylation) is another important posttranslational modification involved in regulation of tau pathophysiology. Among the benefits of O-GlcNAcylation, modulation of tau phosphorylation levels and inhibition of tau aggregation properties have been described while decreased O-GlcNAcylation could be involved in the raise of tau phosphorylation associated with AD. However, the molecular mechanisms at the basis of these observations remain to be defined. In this study, we identify by NMR spectroscopy O-GlcNAc sites in the longest isoform of tau and investigate the direct role of O-GlcNAcylation on tau phosphorylation and conversely, the role of phosphorylation on tau O-GlcNAcylation. We show here by a systematic examination of the quantitative modification patterns by NMR spectroscopy that O-GlcNAcylation does not modify phosphorylation of tau by the kinase activity of ERK2 or a rat brain extract while phosphorylation slightly increases tau O-GlcNAcylation by OGT. Our data suggest that indirect mechanisms act in the reciprocal regulation of tau phosphorylation and O-GlcNAcylation in vivo involving regulation of the enzymes responsible of phosphate and O-GlcNAc dynamics.
Published: 2018
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5. Structural basis for oxygen degradation domain selectivity of the HIF prolyl hydroxylases

Author: Rasheduzzaman Chowdhury, Ivanhoe K. H. Leung, Ya-Min Tian, Martine I. Abboud, Wei Ge, Carmen Domene, François-Xavier Cantrelle, Isabelle Landrieu, Adam P. Hardy, Christopher W. Pugh, Peter J. Ratcliffe, Timothy D. W. Claridge, and Christopher J. Schofield
Subjects: Science
Abstract: The response to hypoxia involves multiple genes regulated by the hypoxia inducible transcription factors (HIFs), whose stability is regulated by prolyl hydroxylation. Here the authors provide a molecular basis for the substrate selectivity of the HIF prolyl hydroxylases that can be altered in erythrocytosis and cancer.
Published: 2016
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6. Backbone NMR resonance assignment of the apo human Tsg101-UEV domain

Author: Danai Moschidi, François-Xavier Cantrelle, Emmanuelle Boll, and Xavier Hanoulle
Subjects: Structural Biology, Biochemistry
Published: 2023

7. Conformation and Affinity Modulations by Multiple Phosphorylation Occurring in the BIN1 SH3 Domain Binding Site of the Tau Protein Proline-Rich Region

Author: Alessia Lasorsa, Krishnendu Bera, Idir Malki, Elian Dupré, François-Xavier Cantrelle, Hamida Merzougui, Davy Sinnaeve, Xavier Hanoulle, Jozef Hritz, Isabelle Landrieu, Biologie Structurale Intégrative (ERL 9002 - BSI ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), CHU Lille, Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Masaryk University [Brno] (MUNI), ANR-15-CE16-0002,BINALZ,Interaction entre BIN1 et Tau: consequences physiologiques et pathophysiologiques pour la maladie d'Alzheimer(2015), and ANR-11-LABX-0009,DISTALZ,Développement de stratégies innovantes pour une approche transdisciplinaire de la maladie d'Alzheime(2011)
Subjects: phosphorylation, protein-protein interactions, intrinsically disordered proteins, Alzheimer's disease, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Biochemistry, tau protein, molecular dynamics, nuclear magnetic resonance spectroscopy
Abstract: International audience; An increase in phosphorylation of the Tau protein is associated with Alzheimer's disease (AD) progression through unclear molecular mechanisms. In general, phosphorylation modifies the interaction of intrinsically disordered proteins, such as Tau, with other proteins; however, elucidating the structural basis of this regulation mechanism remains challenging. The bridging integrator-1 gene is an AD genetic determinant whose gene product, BIN1, directly interacts with Tau. The proline-rich motif recognized within a Tau(210-240) peptide by the SH3 domain of BIN1 (BIN1 SH3) is defined as 216PTPP219, and this interaction is modulated by phosphorylation. Phosphorylation of T217 within the Tau(210-240) peptide led to a 6-fold reduction in the affinity, while single phosphorylation at either T212, T231, or S235 had no effect on the interaction. Nonetheless, combined phosphorylation of T231 and S235 led to a 3-fold reduction in the affinity, although these phosphorylations are not within the BIN1 SH3-bound region of the Tau peptide. Using nuclear magnetic resonance (NMR) spectroscopy, these phosphorylations were shown to affect the local secondary structure and dynamics of the Tau(210-240) peptide. Models of the (un)phosphorylated peptides were obtained from molecular dynamics (MD) simulation validated by experimental data and showed compaction of the phosphorylated peptide due to increased salt bridge formation. This dynamic folding might indirectly impact the BIN1 SH3 binding by a decreased accessibility of the binding site. Regulation of the binding might thus not only be due to local electrostatic or steric effects from phosphorylation but also to the modification of the conformational properties of Tau.
Published: 2023

8. A single domain antibody targeting Tau nucleation core inhibits Tau seeding

Author: Clément Danis, Elian Dupré, Orgeta Zejneli, Raphaëlle Caillierez, Alexis Arrial, Séverine Bégard, Anne Loyens, Justine Mortelecque, François-Xavier Cantrelle, Xavier Hanoulle, Jean-Christophe Rain, Morvane Colin, Luc Buée, and Isabelle Landrieu
Published: 2023

9. Hepatitis E virus RNA‐dependent RNA polymerase is involved in RNA replication and infectious particle production

Author: Jérôme Gouttenoire, Noémie Oechslin, Nathalie Da Silva, François-Xavier Cantrelle, Xavier Hanoulle, Darius Moradpour, Dagmara Szkolnicka, Université de Lausanne = University of Lausanne (UNIL), Biologie Structurale Intégrative (ERL 9002 - BSI ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), This work was supported by the Swiss National Science Foundation (31003A-179424 to DM and CRSK-3_190706 to JG), the Novartis Foundation (18C140 to DM)as well as the French Agency for Research on AIDS and Viral Hepatitis (ECTZ101316 to XH). The NMR facilities were funded by the North Region Council, CNRS, European Union (FEDER), French Research Ministry and University of Lille., HANOULLE, Xavier, and Université de Lausanne (UNIL)
Subjects: Protein Conformation, alpha-Helical, RdRp, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], viruses, particle production, RNA-dependent RNA polymerase, Biology, Virus Replication, medicine.disease_cause, Genome, Virus, Structure-Activity Relationship, reverse genetics, 03 medical and health sciences, chemistry.chemical_compound, Hepatitis E virus, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RNA polymerase, medicine, Humans, replicase, 030304 developmental biology, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 0303 health sciences, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Hepatology, 030306 microbiology, thumb subdomain, RNA, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Hep G2 Cells, RNA-Dependent RNA Polymerase, Virology, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Reverse genetics, Hepatitis E, Open reading frame, chemistry, HEV, Mutation, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RNA, Viral, replicon
Abstract: International audience; Background and Aims: Hepatitis E virus (HEV) is one of the most common causes of acute hepatitis worldwide. Its positive-strand RNA genome encodes three open reading frames (ORF). ORF1 is translated into a large protein composed of multiple domains and known as the viral replicase. The RNA-dependent RNA polymerase (RdRp) domain is responsible for the synthesis of viral RNA.Approach and Results: Here, we identified a highly conserved α-helix located in the RdRp thumb subdomain. Nuclear magnetic resonance demonstrated an amphipathic α-helix extending from amino acids 1628 to 1644 of the ORF1 protein. Functional analyses revealed a dual role of this helix in HEV RNA replication and virus production, including assembly and release. Mutations on the hydrophobic side of the amphipathic α-helix impaired RNA replication and resulted in the selection of a second-site compensatory change in the RdRp palm subdomain. Other mutations enhanced RNA replication but impaired virus assembly and/or release.Conclusions: Structure-function analyses identified a conserved amphipathic α-helix in the thumb subdomain of the HEV RdRp with a dual role in viral RNA replication and infectious particle production. This study provides structural insights into a key segment of the ORF1 protein and describes the successful use of reverse genetics in HEV, revealing functional interactions between the RdRp thumb and palm subdomains. On a broader scale, it demonstrates that the HEV replicase, similar to those of other positive-strand RNA viruses, is also involved in virus production
Published: 2021

10. Dynamic interactions and Ca2+-binding modulate the holdase-type chaperone activity of S100B preventing tau aggregation and seeding

Author: Urmi Sengupta, Guilherme G. Moreira, Ana P. Carapeto, Filipa S. Carvalho, Andrea Quezada, Mário Rodrigues, Isabel Cardoso, Guenter Fritz, Nicha Puangmalai, Isabelle Landrieu, Federico Herrera, Rakez Kayed, Cláudio M. Gomes, Joana S. Cristóvão, François Xavier Cantrelle, Universidade de Lisboa = University of Lisbon (ULISBOA), Biologie Structurale Intégrative (ERL 9002 - BSI ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), The University of Texas Medical Branch (UTMB), Universidade do Porto = University of Porto, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), University of Hohenheim, This work was funded by Fundação para a Ciência e Tecnologia (Portugal) through research grants PTDC/NEU-NMC/2138/2014 (to C.M.G.), PTDC/BIA-BQM/29963/2017 (F.S.C.), PTDC/MED-NEU/31417/2017 (to F.H.), and POCI-01-0145-FEDER-007274 (to I.C.), investigator grants CEECIND/00031/2017 (to A.P.C.) and IF/00094/2013/CP1173/CT0005 (to F.H.), PhD fellowship SFRH/BD/101171/2014 (to J.S.C.) and DFA/BD/6443/2020 (to G.G.M.), and center grants UIDB/04046/2020 and UID/MULTI/04046/2020 (to BioISI) and Norte-01-0145-FEDER-000008 (to IBMC/I3S)., Landrieu, Isabelle, Universidade de Lisboa (ULISBOA), and Universidade do Porto
Subjects: [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Science, Tau protein, General Physics and Astronomy, tau Proteins, S100 Calcium Binding Protein beta Subunit, Protein Aggregation, Pathological, Article, Biophysical Phenomena, General Biochemistry, Genetics and Molecular Biology, Cell Line, 03 medical and health sciences, 0302 clinical medicine, Microtubule, Biophysical chemistry, Calcium-binding protein, mental disorders, Humans, Protein folding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Nuclear Magnetic Resonance, Biomolecular, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], 030304 developmental biology, 0303 health sciences, Multidisciplinary, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Chemistry, Neurodegenerative Diseases, General Chemistry, Kinetics, Proteostasis, Structural biology, Chaperone (protein), biology.protein, Biophysics, Protein Structural Elements, 030217 neurology & neurosurgery, Molecular Chaperones, Protein Binding, Binding domain
Abstract: The microtubule-associated protein tau is implicated in the formation of oligomers and fibrillar aggregates that evade proteostasis control and spread from cell-to-cell. Tau pathology is accompanied by sustained neuroinflammation and, while the release of alarmin mediators aggravates disease at late stages, early inflammatory responses encompass protective functions. This is the case of the Ca2+-binding S100B protein, an astrocytic alarmin which is augmented in AD and which has been recently implicated as a proteostasis regulator, acting over amyloid β aggregation. Here we report the activity of S100B as a suppressor of tau aggregation and seeding, operating at sub-stoichiometric conditions. We show that S100B interacts with tau in living cells even in microtubule-destabilizing conditions. Structural analysis revealed that tau undergoes dynamic interactions with S100B, in a Ca2+-dependent manner, notably with the aggregation prone repeat segments at the microtubule binding regions. This interaction involves contacts of tau with a cleft formed at the interface of the S100B dimer. Kinetic and mechanistic analysis revealed that S100B inhibits the aggregation of both full-length tau and of the microtubule binding domain, and that this proceeds through effects over primary and secondary nucleation, as confirmed by seeding assays and direct observation of S100B binding to tau oligomers and fibrils. In agreement with a role as an extracellular chaperone and its accumulation near tau positive inclusions, we show that S100B blocks proteopathic tau seeding. Together, our findings establish tau as a client of the S100B chaperone, providing evidence for neuro-protective functions of this inflammatory mediator across different tauopathies., The calcium binding protein S100B is an abundantly expressed protein in the brain and has neuro-protective functions by inhibiting Aβ aggregation and metal ion toxicity. Here, the authors combine cell biology and biochemical experiments with chemical kinetics and NMR measurements and show that S100B protein is an extracellular Tau chaperone and further characterize the interactions between S100B and Tau.
Published: 2021

11. Novel dithiocarbamates selectively inhibit 3CL protease of SARS-CoV-2 and other coronaviruses

Author: Lucile Brier, Haitham Hassan, Xavier Hanoulle, Valerie Landry, Danai Moschidi, Lowiese Desmarets, Yves Rouillé, Julie Dumont, Adrien Herledan, Sandrine Warenghem, Catherine Piveteau, Paul Carré, Sarah Ikherbane, François-Xavier Cantrelle, Elian Dupré, Jean Dubuisson, Sandrine Belouzard, Florence Leroux, Benoit Deprez, Julie Charton, Médicaments et molécules pour agir sur les Systèmes Vivants - U 1177 (M2SV), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Université de Bordeaux (UB), Biologie Structurale Intégrative (ERL 9002 - BSI ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), CHU Lille, Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 (PLBS), and Réseau International des Instituts Pasteur (RIIP)
Subjects: Coronavirus, 3CL protease, Pharmacology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], SARS-CoV-2, Inhibitors, Organic Chemistry, Drug Discovery, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, General Medicine, Antiviral
Abstract: International audience; Since end of 2019, the global and unprecedented outbreak caused by the coronavirus SARS-CoV-2 led to dramatic numbers of infections and deaths worldwide. SARS-CoV-2 produces two large viral polyproteins which are cleaved by two cysteine proteases encoded by the virus, the 3CL protease (3CLpro) and the papain-like protease, to generate non-structural proteins essential for the virus life cycle. Both proteases are recognized as promising drug targets for the development of anti-coronavirus chemotherapy. Aiming at identifying broad spectrum agents for the treatment of COVID-19 but also to fight emergent coronaviruses, we focused on 3CLpro that is well conserved within this viral family. Here we present a high-throughput screening of more than 89,000 small molecules that led to the identification of a new chemotype, potent inhibitor of the SARS-CoV-2 3CLpro. The mechanism of inhibition, the interaction with the protease using NMR and X-Ray, the specificity against host cysteine proteases and promising antiviral properties in cells are reported.
Published: 2023

12. Inhibition of Tau seeding by targeting Tau nucleation core within neurons with a single domain antibody fragment

Author: Clément Danis, Elian Dupré, Orgeta Zejneli, Raphaëlle Caillierez, Alexis Arrial, Séverine Bégard, Justine Mortelecque, Sabiha Eddarkaoui, Anne Loyens, François-Xavier Cantrelle, Xavier Hanoulle, Jean-Christophe Rain, Morvane Colin, Luc Buée, Isabelle Landrieu, Biologie Structurale Intégrative (ERL 9002 - BSI ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Lille Neurosciences & Cognition - U 1172 (LilNCog), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Hybrigenics [Paris], Hybrigenics, ANR-18-CE44-0016,ToNIC,Caractérisation moléculaire et cellulaire de nanobodies dirigés contre Tau(2018), and Landrieu, Isabelle
Subjects: Neurons, Pharmacology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], tau Proteins, [SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy, Single-Domain Antibodies, Repressor Proteins, Disease Models, Animal, Mice, Tauopathies, Alzheimer Disease, Drug Discovery, Genetics, Animals, Molecular Medicine, [SDV.IMM.IMM] Life Sciences [q-bio]/Immunology/Immunotherapy, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS
Abstract: Tau proteins aggregate into filaments in brain cells in Alzheimer's disease and related disorders referred to as tauopathies. Here, we used fragments of camelid heavy-chain-only antibodies (VHHs or single domain antibody fragments) targeting Tau as immuno-modulators of its pathologic seeding. A VHH issued from the screen against Tau of a synthetic phage-display library of humanized VHHs was selected for its capacity to bind Tau microtubule-binding domain, composing the core of Tau fibrils. This parent VHH was optimized to improve its biochemical properties and to act in the intra-cellular compartment, resulting in VHH Z70. VHH Z70 precisely binds the PHF6 sequence, known for its nucleation capacity, as shown by the crystal structure of the complex. VHH Z70 was more efficient than the parent VHH to inhibit in vitro Tau aggregation in heparin-induced assays. Expression of VHH Z70 in a cellular model of Tau seeding also decreased the aggregation-reporting fluorescence signal. Finally, intra-cellular expression of VHH Z70 in the brain of an established tauopathy mouse seeding model demonstrated its capacity to mitigate accumulation of pathological Tau. VHH Z70, by targeting Tau inside brain neurons, where most of the pathological Tau resides, provides an immunological tool to target the intra-cellular compartment in tauopathies.
Published: 2022

13. NMR spectroscopy of the main protease of SARS‐CoV‐2 and fragment‐based screening identify three protein hotspots and an antiviral fragment

Author: Florence Leroux, Julie Charton, François-Xavier Cantrelle, Xavier Hanoulle, Sandrine Belouzard, Danai Moschidi, Isabelle Landrieu, Lucile Brier, Jean Dubuisson, Valérie Landry, Emmanuelle Boll, Benoit Deprez, Frédérique Dewitte, Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Biologie Structurale Intégrative (ERL 9002 - BSI ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Médicaments et molécules pour agir sur les Systèmes Vivants - U 1177 (M2SV), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Projet 3CLPRO-SCREEN-NMR (I-SITE Université Lille Nord-Europe)Institut Pasteur de Lille, HANOULLE, Xavier, Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)
Subjects: Protein Conformation, medicine.medical_treatment, Drug Evaluation, Preclinical, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 01 natural sciences, Protein structure, Chlorocebus aethiops, Coronavirus 3C Proteases, Research Articles, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 0303 health sciences, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Drug discovery, Chemistry, fragment screening, Nuclear magnetic resonance spectroscopy, General Medicine, 3. Good health, Protein Structure | Hot Paper, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Research Article, Stereochemistry, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], [CHIM.THER] Chemical Sciences/Medicinal Chemistry, Allosteric regulation, Context (language use), Microbial Sensitivity Tests, Cysteine Proteinase Inhibitors, 010402 general chemistry, Antiviral Agents, Catalysis, drug discovery, Small Molecule Libraries, 03 medical and health sciences, NMR spectroscopy, medicine, Animals, viruses, Binding site, protein structure, Nuclear Magnetic Resonance, Biomolecular, Vero Cells, 030304 developmental biology, Protease, Binding Sites, SARS-CoV-2, 010405 organic chemistry, Active site, General Chemistry, 0104 chemical sciences, biology.protein, Protein Multimerization
Abstract: The main protease (3CLp) of the SARS‐CoV‐2, the causative agent for the COVID‐19 pandemic, is one of the main targets for drug development. To be active, 3CLp relies on a complex interplay between dimerization, active site flexibility, and allosteric regulation. The deciphering of these mechanisms is a crucial step to enable the search for inhibitors. In this context, using NMR spectroscopy, we studied the conformation of dimeric 3CLp from the SARS‐CoV‐2 and monitored ligand binding, based on NMR signal assignments. We performed a fragment‐based screening that led to the identification of 38 fragment hits. Their binding sites showed three hotspots on 3CLp, two in the substrate binding pocket and one at the dimer interface. F01 is a non‐covalent inhibitor of the 3CLp and has antiviral activity in SARS‐CoV‐2 infected cells. This study sheds light on the complex structure‐function relationships of 3CLp and constitutes a strong basis to assist in developing potent 3CLp inhibitors., We report the liquid‐sate NMR spectroscopy analysis of the dimeric SARS‐CoV‐2 main protease (3CLp), including its backbone assignments, to study its complex conformational regulation. Using fragment‐based NMR screening, we highlighted three hotspots on the protein, two in the substrate binding pocket and one at the dimer interface, and we identified a non‐covalent reversible inhibitor of 3CLp that has antiviral activity in infected cells.
Published: 2021

14. Azobenzene: a Visible‐Light Chemical Actinometer for the Characterization of Fluidic Photosystems

Author: Xavier Trivelli, François Xavier Cantrelle, Laëtitia Chausset-Boissarie, Maël Penhoat, Mélanie Roseau, Vincent De Waele, Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - USR 3290 (MSAP), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Institut de Chimie du CNRS (INC), Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille Institut (CLIL), Institut Michel Eugène Chevreul - FR 2638 (IMEC), Université d'Artois (UA)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Biologie Structurale Intégrative (ERL 9002 - BSI ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), The authors gratefully acknowledge the CNRS, Lille University and ANR (ANR-19-CE07-0014) for the financial support., ANR-19-CE07-0014,FloRyn,Photochimie en flux continu et intermédiaires réactifs à courte durée de vie une combinaison gagnante(2019), Chausset-Boissarie, laetitia, Photochimie en flux continu et intermédiaires réactifs à courte durée de vie une combinaison gagnante - - FloRyn2019 - ANR-19-CE07-0014 - AAPG2019 - VALID, Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 (MSAP), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: azo compounds, actinometry, 010402 general chemistry, Photochemistry, 01 natural sciences, Biochemistry, Catalysis, law.invention, Inorganic Chemistry, chemistry.chemical_compound, law, Drug Discovery, [CHIM] Chemical Sciences, [CHIM]Chemical Sciences, Fluidics, Physical and Theoretical Chemistry, microfluidic photoreactor, visible light, Photosystem, Actinometer, photochemistry, 010405 organic chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Organic Chemistry, [CHIM.ORGA] Chemical Sciences/Organic chemistry, 0104 chemical sciences, Characterization (materials science), azobenzene, Azobenzene, chemistry, Visible spectrum
Abstract: International audience; (E)-Azobenzene is introduced as a suitable chemical actinometer in the visible spectral range (440–540 nm) for photon flux determination of fluidic microphotoreactors or for assessing efficiency of visible light photo-induced reactions, its evaluation is straightforward without tedious analytics. Photoisomerization quantum yields (ΦE→Z) of (E)-azobenzene were accurately determined upon irradiation at several wavelengths and in different solvents based on well-known diarylethene.
Published: 2021

15. Set-up and screening of a fragment library targeting the 14-3-3 protein interface

Author: Dimitrios Tzalis, Christian Ottmann, Laura Levy, Domenico Lentini Santo, Stanimira Hristeva, Isabelle Landrieu, João Neves, Tomas Obsil, Dario Valenti, François Xavier Cantrelle, Xavier Hanoulle, Taros Chemicals GmbH & Co. KG, Technische Universiteit Eindhoven (TU/e), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Charles University [Prague] (CU), Universität Duisburg-Essen [Essen], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Charles University [Prague], Chemical Biology, Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Universität Duisburg-Essen = University of Duisburg-Essen [Essen]
Subjects: Pharmacology, Gene isoform, 010405 organic chemistry, Chemistry, Organic Chemistry, Chemie, Pharmaceutical Science, Signal transducing adaptor protein, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Nuclear magnetic resonance spectroscopy, Computational biology, 01 natural sciences, Biochemistry, 0104 chemical sciences, Selective modulation, 010404 medicinal & biomolecular chemistry, Fragment (logic), Drug Discovery, Molecular Medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 14-3-3 protein
Abstract: Protein-protein interactions (PPIs) are at the core of regulation mechanisms in biological systems and consequently became an attractive target for therapeutic intervention. PPIs involving the adapter protein 14-3-3 are representative examples given the broad range of partner proteins forming a complex with one of its seven human isoforms. Given the challenges represented by the nature of these interactions, fragment-based approaches offer a valid alternative for the development of PPI modulators. After having assembled a fragment set tailored on PPIs' modulation, we started a screening campaign on the sigma isoform of 14-3-3 adapter proteins. Through the use of both mono-and bi-dimensional nuclear magnetic resonance spectroscopy measurements, coupled with differential scanning fluorimetry, three fragment hits were identified. These molecules bind the protein at two different regions distant from the usual binding groove highlighting new possibilities for selective modulation of 14-3-3 complexes.
Published: 2019

16. Phosphorylated full-length Tau interacts with 14-3-3 proteins via two short phosphorylated sequences, each occupying a binding groove of 14-3-3 dimer

Author: João Filipe Neves, François Xavier Cantrelle, Tomas Obsil, Isabelle Landrieu, Gavin O'Mahony, Xavier Hanoulle, Olivia Petrvalska, Francesco Bosica, Hamida Merzougui, Chemical Biology, Biologie Structurale Intégrative (ERL 9002 - BSI ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Charles University [Prague] (CU), AstraZeneca, European Project: 675179,H2020,H2020-MSCA-ITN-2015,TASPPI(2016), Landrieu, Isabelle, and Targeted small-molecule Stabilisation of Protein-Protein Interactions - TASPPI - - H20202016-02-01 - 2020-01-31 - 675179 - VALID
Subjects: 0301 basic medicine, Magnetic Resonance Spectroscopy, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Protein Conformation, Dimer, Tau protein, tau Proteins, protein–protein interactions, Biochemistry, Epitope, Protein–protein interaction, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, NMR spectroscopy, Alzheimer Disease, Serine, Humans, Phosphorylation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Binding Sites, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Chemistry, Drug discovery, 14-3-3 proteins, Cell Biology, Nuclear magnetic resonance spectroscopy, Surface Plasmon Resonance, Alzheimer's disease, Cyclic AMP-Dependent Protein Kinases, 030104 developmental biology, Monomer, Protein-Protein Interactions, 030220 oncology & carcinogenesis, Exoribonucleases, Mutation, Biophysics, biology.protein, Protein Multimerization, Alzheimer’s disease, analytical ultracentrifugation, Protein Binding
Abstract: Protein-protein interactions (PPIs) remain poorly explored targets for the treatment of Alzheimer’s disease (AD). The interaction of 14-3-3 proteins with Tau was shown to have detrimental effects on neuronal cells and to be linked to Tau pathology. This PPI is therefore seen as a potential target for AD. When Tau is phosphorylated by PKA (Tau-PKA), two 14-3-3 binding epitopes are generated, surrounding the phosphorylated serines 214 and 324 of Tau. The crystal structures of 14-3-3 in complex with peptides surrounding these Tau phosphosites show that both these motifs are anchored in the amphipathic binding groove of 14-3-3. However, in the absence of structural data with the full-length Tau protein, the stoichiometry of the complex or the interface and affinity of the partners, are still unclear. In this work, we addressed these points, using a broad range of biophysical techniques. The interaction of the long disordered Tau-PKA protein with 14-3-3σ is restricted to two short sequences, containing phosphorylated serines, which bind in the amphipathic binding groove of 14-3-3. Phosphorylation of Tau is fundamental for the formation of this stable complex, and the affinity of the Tau-PKA/14-3-3 interaction is in the 1-10 micromolar range. Each monomer of the 14-3-3σ dimer binds one of the two different phosphorylated peptides of Tau-PKA, suggesting a 14-3-3/Tau-PKA stoichiometry of 2:1, confirmed by analytical ultracentrifugation. These results contribute to a better understanding of this PPI and provide useful insights for drug discovery projects aiming at the inhibition of this interaction.
Published: 2021

17. A Novel Natural Siderophore Antibiotic Conjugate Reveals a Chemical Approach to Macromolecule Coupling

Author: Thibault Caradec, Ernesto Anoz-Carbonell, Ravil Petrov, Muriel Billamboz, Kevin Antraygues, Francois-Xavier Cantrelle, Emmanuelle Boll, Delphine Beury, David Hot, Herve Drobecq, Xavier Trivelli, and Ruben C. Hartkoorn
Subjects: Chemistry, QD1-999
Published: 2023
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18. The O-β-linked N-acetylglucosaminylation of the Lamin B receptor and its impact on DNA binding and phosphorylation

Author: Caroline Smet-Nocca, Eleni Nikolakaki, Isabelle Landrieu, Adeline Page, François-Xavier Cantrelle, Thomas Giannakouros, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS), Aristotelian University of Thessaloniki, Aristotle University of Thessaloniki, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Turkeys, Glycosylation, Proto-Oncogene Proteins c-akt, [SDV]Life Sciences [q-bio], Biophysics, Receptors, Cytoplasmic and Nuclear, Lamin B receptor, N-Acetylglucosaminyltransferases, Biochemistry, Acetylglucosamine, 03 medical and health sciences, CDC2 Protein Kinase, Animals, Humans, Inner membrane, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Protein phosphorylation, Amino Acid Sequence, Phosphorylation, Nuclear protein, Molecular Biology, Integral membrane protein, ComputingMilieux_MISCELLANEOUS, Binding Sites, Sequence Homology, Amino Acid, Chemistry, DNA, Rats, Chromatin, Cell biology, 030104 developmental biology, Peptides, Protein Binding
Abstract: Lamin B Receptor (LBR) is an integral protein of the interphase inner nuclear membrane that is implicated in chromatin anchorage to the nuclear envelope. Phosphorylation of a stretch of arginine-serine (RS) dipeptides in the amino-terminal nucleoplasmic domain of LBR regulates the interactions of the receptor with other nuclear proteins, DNA and RNA and thus modulates tethering of heterochromatin to the nuclear envelope. While phosphorylation has been extensively studied, very little is known about other post-translational modifications of the protein. There is only one report on the O-β-linked N-acetyl-glucosaminylation (O-GlcNAcylation) of a serine residue downstream of the RS domain of rat LBR. In the present study we identify additional O-GlcNAcylation sites by using as substrates of O-β-N-acetylglucosaminyltransferase (OGT) a set of peptides containing the entire LBR RS domain or parts of it as well as flanking sequences. The in vitro activity of OGT was assessed by tandem mass spectrometry and NMR spectroscopy. Furthermore, we provide evidence that O-GlcNAcylation hampers DNA binding while it marginally affects RS domain phosphorylation mediated by SRPK1, Akt2 and cdk1 kinases. General significance Our methodology providing a quantitative description of O-GlcNAc patterns based on a combination of mass spectrometry and high resolution NMR spectroscopy on short peptide substrates allows subsequent functional analyses. Hence, our approach is of general interest to a wide audience of biologists aiming at deciphering the functional role of O-GlcNAc glycosylation and its crosstalk with phosphorylation.
Published: 2018

19. Overall Structural Model of NS5A Protein from Hepatitis C Virus and Modulation by Mutations Confering Resistance of Virus Replication to Cyclosporin A

Author: Stéphane Sarrazin, Véronique Receveur-Bréchot, Guy Lippens, Marie-Laure Fogeron, Ralf Bartenschlager, Roland Montserret, Sylvie Ricard-Blum, Volker Lohmann, Aurélie Badillo, Jennifer Molle, François-Xavier Cantrelle, Xavier Hanoulle, François Penin, Anja Böckmann, Frédéric Delolme, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Department of Infectious Diseases [Heidelberg, Germany], Heidelberg University Hospital [Heidelberg], Agence Nationale de Recherches sur le Sida et les Hepatites Virales (ANRS) [A02007-2, A02011-2], French National Agency for Research [ANR-11-JSV8-005], [LABEX ECOFECT ANR-11-LABX-0048], French National Agency for Research (MAPPING Project) [ANR-11-BINF-0003], Deutsche Forschungsgemeinschaft [SFB/TRR83 TP13], CNRS (TGIR RMN THC) [FR-3050], Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)
Subjects: Models, Molecular, 0301 basic medicine, Protein Conformation, [SDV]Life Sciences [q-bio], Hepatitis C virus, Hepacivirus, Microbial Sensitivity Tests, Viral Nonstructural Proteins, Biology, Virus Replication, medicine.disease_cause, Antiviral Agents, Biochemistry, Mass Spectrometry, 03 medical and health sciences, Protein structure, Cyclosporin a, Drug Resistance, Viral, Genotype, medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, NS5A, Mutation, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], 3. Good health, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 030104 developmental biology, Viral replication, Phosphoprotein, Cyclosporine
Abstract: International audience; Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is a RNA-binding phosphoprotein composed of a N-terminal membrane anchor (AH), a structured domain 1 (D1), and two intrinsically disordered domains (D2 and D3). The knowledge of the functional architecture of this multifunctional protein remains limited. We report here that NS5A-D1D2D3 produced in a wheat germ cell-free system is obtained under a highly phosphorylated state. Its NMR analysis revealed that these phosphorylations do not change the disordered nature of D2 and D3 domains but increase the number of conformers due to partial phosphorylations. By combining NMR and small angle X-ray scattering, we performed a comparative structural characterization of unphosphorylated recombinant D2 domains of JFH1 (genotype 2a) and the Con1 (genotype 1b) strains produced in Escherichia coli. These analyses highlighted a higher intrinsic folding of the latter, revealing the variability of intrinsic conformations in HCV genotypes. We also investigated the effect of D2 mutations conferring resistance of HCV replication to cyclophilin A (CypA) inhibitors on the structure of the recombinant D2 Con1 mutants and their binding to CypA. Although resistance mutations D320E and R318W could induce some local and/or global folding perturbation, which could thus affect the kinetics of conformer interconversions, they do not significantly affect the kinetics of CypA/D2 interaction measured by surface plasmon resonance (SPR). The combination of all our data led us to build a model of the overall structure of NS5A, which provides a useful template for further investigations of the structural and functional features of this enigmatic protein.
Published: 2017

20. Metabolic phenotyping of human plasma by1H-NMR at high and medium magnetic field strengths: a case study for lung cancer

Author: Liesbet Mesotten, Peter Adriaensens, Michiel Thomeer, Karolien Vanhove, François-Xavier Cantrelle, Gunter Reekmans, Guy Lippens, Liene Bervoets, and Evelyne Louis
Subjects: 0301 basic medicine, Field (physics), Spectrometer, Chemistry, General Chemistry, Nuclear magnetic resonance spectroscopy, equipment and supplies, Spectral line, Magnetic field, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Metabolomics, Nuclear magnetic resonance, 030220 oncology & carcinogenesis, Proton NMR, General Materials Science, Spectral resolution
Abstract: Accurate identification and quantification of human plasma metabolites can be challenging in crowded regions of the NMR spectrum with severe signal overlap. Therefore, this study describes metabolite spiking experiments on the basis of which the NMR spectrum can be rationally segmented into well-defined integration regions, and this for spectrometers having magnetic field strengths corresponding to 1 H resonance frequencies of 400 MHz and 900 MHz. Subsequently, the integration data of a case-control dataset of 69 lung cancer patients and 74 controls were used to train a multivariate statistical classification model for both field strengths. In this way, the advantages/disadvantages of high versus medium magnetic field strength were evaluated. The discriminative power obtained from the data collected at the two magnetic field strengths is rather similar, i.e. a sensitivity and specificity of respectively 90 and 97% for the 400 MHz data versus 88 and 96% for the 900 MHz data. This shows that a medium-field NMR spectrometer (400-600 MHz) is already sufficient to perform clinical metabolomics. However, the improved spectral resolution (reduced signal overlap) and signal-to-noise ratio of 900 MHz spectra yield more integration regions that represent a single metabolite. This will simplify the unraveling and understanding of the related, disease disturbed, biochemical pathways. Copyright © 2017 John Wiley & Sons, Ltd.
Published: 2017

21. Cyclophilin A allows the allosteric regulation of a structural motif in the disordered domain 2 of NS5A and thereby fine-tunes HCV RNA replication

Author: François-Xavier Cantrelle, Xavier Hanoulle, Ralf Bartenschlager, Marie Dujardin, Guy Lippens, Vanesa Madan, Isabelle Huvent, Hélène Launay, Neha S. Gandhi, Université de Lille, Universität Heidelberg [Heidelberg] = Heidelberg University, School of Mathematical Sciences, Queensland University of Technology, School of Mathematical Sciences [Brisbane], Queensland University of Technology [Brisbane] (QUT)-Queensland University of Technology [Brisbane] (QUT), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Department of Molecular Virology (DMV), Toulouse Biotechnology Institute (TBI), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), ANRS French National Research Agency (ANR) A02014-2, Lille University, German Research Foundation (DFG) 112927078-TRR 83, Research Federation 'structural and functional biochemistry of BIOmolecular Assemblies' (CNRS) FR-3688, ANR-11-JSV8-0005,StruFunc5A5B,Etude des relations structurales et fonctionnelles entre les protéines NS5A et NS5B du virus de l'hépatite C et la Cyclophiline A humaine.(2011), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Universität Heidelberg [Heidelberg], Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Synthèse, structure et fonction des biomolécules (SSFB), Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), CNRS, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, nuclear magnetic resonance (NMR), [SDV]Life Sciences [q-bio], Allosteric regulation, prolyl isomerase, Cypa, nonstructural protein 5A (NS5A), Molecular Dynamics Simulation, Viral Nonstructural Proteins, Virus Replication, Intrinsically disordered proteins, Biochemistry, 03 medical and health sciences, Cyclophilin A, conformer ensemble, Prolyl isomerase, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Structural motif, NS5A, Nuclear Magnetic Resonance, Biomolecular, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Cyclophilin, intrinsically disordered protein, hepatitis C virus (HCV), cyclophilin, allosteric regulation, RNA replication, 030102 biochemistry & molecular biology, biology, Chemistry, 030402 Biomolecular Modelling and Design, Cell Biology, biology.organism_classification, 3. Good health, Cell biology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, 030104 developmental biology, 030406 Proteins and Peptides, Protein Structure and Folding, RNA, Viral, 060112 Structural Biology (incl. Macromolecular Modelling)
Abstract: International audience; Implicated in numerous human diseases, intrinsically disordered proteins (IDPs) are dynamic ensembles of interconverting conformers that often contain many proline residues. Whether and how proline conformation regulates the functional aspects of IDPs remains an open question, however. Here, we studied the disordered domain 2 of nonstructural protein 5A (NS5A-D2) of hepatitis C virus (HCV). NS5A-D2 comprises a short structural motif (PW-turn) embedded in a proline-rich sequence , whose interaction with the human prolyl isomerase cyclophilin A (CypA) is essential for viral RNA replication. Using NMR, we show here that the PW-turn motif exists in a conformational equilibrium between folded and disordered states. We found that the fraction of conformers in the NS5A-D2 ensemble that adopt the structured motif is allosteri-cally modulated both by the cis/trans isomerization of the surrounding prolines that are CypA substrates and by substitutions conferring resistance to cyclophilin inhibitor. Moreover, we noted that this fraction is directly correlated with HCV RNA replication efficiency. We conclude that CypA can fine-tune the dynamic ensemble of the disordered NS5A-D2, thereby regulating viral RNA replication efficiency.
Published: 2019

22. Single Domain Antibody Fragments as New Tools for the Detection of Neuronal Tau Protein in Cells and in Mice Studies

Author: Clément Danis, Morvane Colin, Isabelle Landrieu, Elian Dupre, Jean-Christophe Rain, François-Xavier Cantrelle, Mégane Homa, Hamida Merzougui, Alexis Arrial, Luc Buée, Xavier Hanoulle, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Hybrigenics [Paris], Hybrigenics, Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, ANR-16-IDEX-0004,ULNE,ULNE(2016), ANR-18-CE44-0016,ToNIC,Caractérisation moléculaire et cellulaire de nanobodies dirigés contre Tau(2018), Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer (JPArc - U1172 Inserm), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université Lille 2 - Faculté de Médecine, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), Centre de recherche Jean-Pierre Aubert-Neurosciences et Cancer, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, and Landrieu, Isabelle
Subjects: Genetically modified mouse, Physiology, Cognitive Neuroscience, [SDV]Life Sciences [q-bio], Tau protein, Variable domain of the heavy-chain only antibodies, Mice, Transgenic, tau Proteins, Context (language use), Biochemistry, Epitope, Mice, 03 medical and health sciences, 0302 clinical medicine, Alzheimer Disease, Cell Line, Tumor, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, Immunoglobulin Fragments, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, nuclear magnetic resonance spectroscopy, Neurons, 0303 health sciences, biology, Chemistry, tauopathies, Cell Biology, General Medicine, Single-Domain Antibodies, Alzheimer's disease, In vitro, nanobodies, Cell biology, [SDV] Life Sciences [q-bio], Single-domain antibody, Cell culture, biology.protein, Antibody, 030217 neurology & neurosurgery
Abstract: Tau is a neuronal protein linked to pathologies called tauopathies, including Alzheimer's disease. In Alzheimer's disease, tau aggregates into filaments, leading to the observation of intraneuronal fibrillary tangles. Molecular mechanisms resulting in tau aggregation and in tau pathology spreading through the brain regions are still not fully understood. New tools are thus needed to decipher tau pathways involved in the diseases. In this context, a family of novel single domain antibody fragments, or VHHs, directed against tau were generated and characterized. Among the selected VHHs obtained from screening of a synthetic library, a family of six VHHs shared the same CDR3 recognition loop and recognized the same epitope, located in the C-terminal domain of tau. Affinity parameters characterizing the tau/VHHs interaction were next evaluated using surface plasmon resonance spectroscopy. The equilibrium constants KD were in the micromolar range, but despite conservation of the CDR3 loop sequence, a range of affinities was observed for this VHH family. One of these VHHs, named F8-2, was additionally shown to bind tau upon expression in a neuronal cell line model. Optimization of VHH F8-2 by yeast two-hybrid allowed the generation of an optimized VHH family characterized by lower KD than that of the F8-2 wild-type counterpart, and recognizing the same epitope. The optimized VHHs can also be used as antibodies for detecting tau in transgenic mice brain tissues. These results validate the use of these VHHs for in vitro studies, but also their potential for in-cell expression and assays in mouse models, to explore the mechanisms underlying tau physiopathology.
Published: 2019

23. Additional file 10: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Abstract: Co-localization measurement of BIN1-Tau complexes with actin, clathrin-coated membranes and synapse terminal markers. PLA Tau-BIN1 staining (green) with (A) actin staining, (B) clathrin staining, (C) the pre-synaptic marker synaptophysin and (D) the post-synaptic marker PSD95 (red) in primary neuron cultures. The right-hand panels show the pixels with co-localization (in white) of PLA and the various markers. The co-localization coefficient was calculated according to Mander’s method using ZEN 2012 software. The red channel was used as a reference. E. A graph showing the mean ± SD (error bar) coefficient for co-localization between PLA BIN1/Tau and the indicated markers (n = 6). For details of the methods, see Additional file 12. (PDF 3475 kb)
Published: 2019
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24. Additional file 5: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Abstract: Phosphorylation of Tau with the CDK2/CycA3 kinase. A. Details of 2D [1H, 15N] HSQC spectra of 125 μM 15N Tau[165–245] (Tau-F5) phosphorylated with CDK2/CycA3 kinase. B. Details of 2D [1H, 15N] HSQC spectra of 100 μM 15N Tau phosphorylated with CDK2/CycA3 kinase. Shifted resonances corresponding to phosphorylated Ser and Thr residues are labelled [29]. Resonances located within the SH3 binding site are annotated in red. (PDF 76 kb)
Published: 2019
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25. Additional file 8: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Abstract: Interaction of GST-BIN1/SH3 with phospho-Tau. A. In vitro phosphorylation of Tau by ERK kinase. Details of 2D [1H, 15N] HSQC spectra of 15N Tau-F5 [165–245] phosphorylated with ERK kinase. Shifted resonances corresponding to phosphorylated Ser and Thr residues are labelled [29]. Resonances of pT231 and pS235 are broader and less intense. Resonances located within the SH3 binding site are annotated in red. B. Interaction of GST BIN1/SH3 with phospho Tau-F5. HSQC spectra of 125 μM 15N ERK-phosphorylated Tau-F5 [165–245] free in solution (gray) and with a 1 molar amount of GST-BIN1/SH3(red, superimposed) C. Overlaid detail of 2D [1H, 15N] HSQC spectra presented in B. For details of the methods, see Additional file 12. (PDF 123 kb)
Published: 2019
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26. Additional file 9: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Subjects: macromolecular substances, environment and public health
Abstract: Interaction of GST-BIN1/SH3 with phospho-Tau. A. in vitro phosphorylation of Tau by rat brain extracts. Details of 2D [1H, 15N] HSQC spectra of 15N Tau phosphorylated with rat brain extracts. Shifted resonances corresponding to phosphorylated Ser and Thr residues are labelled [29]. Resonances of pT231 and pS235 are broader and less intense. Resonances located within the SH3 binding site are annotated in red. B. Interaction of phosphorylated Tau FL with GST-BIN1/SH3. 2D [1H, 15N] HSQC spectra of 100 μM 15N Tau phosphorylated with rat brain extract, free in solution (gray) or with a 1.6 molar amount of GST-BIN1/SH3 (blue, superimposed). C. Overlaid detail of 2D [1H, 15N] HSQC spectra presented in B. For details of the methods, see Additional file 12. (PDF 137 kb)
Published: 2019
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27. Additional file 7: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Abstract: Tau phosphorylation precludes the BIN1-Tau interaction in vitro. 2D [1H, 15N] HSQC spectra of 100 μM 15N CDK-phosphorylated 2N4R Tau, free in solution (gray) or with a 1.6 molar amount of GST-BIN1/SH3 (blue, superimposed). No CS perturbations or peak broadening were observed – indicating the absence of interaction between BIN1 and Tau Fl. (PDF 82 kb)
Published: 2019
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28. Additional file 4: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Abstract: BIN1-Tau interaction is mediated by the Tau sequence from aa 212 to aa 231. Overlaid detail of 2D [1H, 15N] HSQC spectra of 100 μM 15N Tau-F5 [165–245] free in solution (gray) or with a 1.2 molar amount of GST-BIN1/SH3 (red, superimposed). Blue arrows link the corresponding resonances in the free (gray) and bound (red) states. (PDF 74 kb)
Published: 2019
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29. Additional file 6: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Author: Sottejeau, Yoann, Bretteville, Alexis, François-Xavier Cantrelle, Malmanche, Nicolas, Demiaute, Florie, Mendes, Tiago, Delay, Charlotte, Alves, Harmony Alves Dos, Flaig, Amandine, Davies, Peter, Dourlen, Pierre, Dermaut, Bart, Laporte, Jocelyn, Amouyel, Philippe, Lippens, Guy, Chapuis, Julien, Landrieu, Isabelle, and Jean-Charles Lambert
Abstract: Tau phosphorylation precludes the BIN1-Tau interaction in vitro. 2D [1H, 15N] HSQC spectra of 125 μM 15N CDK-phosphorylated Tau-F5[165–245] free in solution (gray) and with a 1 molar amount of GST-BIN1/SH3 (red, superimposed). No CS perturbations or peak broadening were observed - indicating the absence of interaction between BIN1 and Tau-F5. (PDF 67 kb)
Published: 2019
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30. BIN1 recovers tauopathy-induced long-term memory deficits in mice and interacts with Tau through Thr348 phosphorylation

Author: Mikko Hiltunen, Idir Malki, Alessia Lasorsa, Amandine Flaig, Maxime Sartori, Yann Herault, Shruti Desai, Isabelle Landrieu, Mikael Marttinen, Jean-Charles Lambert, Tiago Mendes, Julien Chapuis, Philippe Amouyel, Jocelyn Laporte, Devrim Kilinc, Benoit Deprez, Anais-Camille Vreulx, Nicolas Malmanche, Laurent Pradier, Petra Mäkinen, Florence Leroux, Adrien Herldean, Damien Marechal, and François-Xavier Cantrelle
Subjects: Genetically modified mouse, 0303 health sciences, Long-term memory, Kinase, Hippocampus, Biology, medicine.disease, 3. Good health, Cell biology, Calcineurin, 03 medical and health sciences, 0302 clinical medicine, medicine, Phosphorylation, Tauopathy, Alzheimer's disease, 030217 neurology & neurosurgery, 030304 developmental biology
Abstract: The bridging integrator 1 gene (BIN1) is a major genetic risk factor for Alzheimer’s disease (AD). In this report, we investigated how BIN1-dependent pathophysiological processes might be associated with Tau. We first generated a cohort of control and transgenic mice either overexpressing human MAPT (TgMAPT) or both human MAPT and BIN1 (TgMAPT;TgBIN1), which we followed-up from 3 to 15 months. In TgMAPT;TgBIN1 mice short-term memory deficits appeared earlier than in TgMAPT mice; however – unlike TgMAPT mice – TgMAPT;TgBIN1 mice did not exhibit any long-term or spatial memory deficits for at least 15 months. After sacrifice of the cohort at 18 months, immunohistochemistry revealed that BIN1 overexpression prevents both Tau mislocalization and somatic inclusion in the hippocampus, where an increase in BIN1-Tau interaction was also observed. We then sought mechanisms controlling the BIN1-Tau interaction. We developed a high-content screening approach to characterize modulators of the BIN1-Tau interaction in an agnostic way (1,126 compounds targeting multiple pathways), and we identified – among others – an inhibitor of Calcineurin, a Ser/Thr phosphatase. We determined that Calcineurin dephosphorylates BIN1 on a Cyclin-dependent kinase phosphorylation site at T348, promoting the open conformation of the neuronal BIN1 isoform. Phosphorylation of this site increases the availability of the BIN1 SH3 domain for Tau interaction, as demonstrated by nuclear magnetic resonance experiments and in primary neurons. Finally, we observed that the levels of the neuronal BIN1 isoform were decreased in AD brains, whereas phospho-BIN1(T348):BIN1 ratio was increased, suggesting a compensatory mechanism. In conclusion, our data support the idea that BIN1 modulates the AD risk through an intricate regulation of its interaction with Tau. Alteration in BIN1 expression or activity may disrupt this regulatory balance with Tau and have direct effects on learning and memory.
Published: 2018
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31. Direct Crosstalk Between

Author: Gwendoline, Bourré, François-Xavier, Cantrelle, Amina, Kamah, Béatrice, Chambraud, Isabelle, Landrieu, and Caroline, Smet-Nocca
Subjects: Endocrinology, O-GlcNAcylation, NMR spectroscopy, phosphorylation, mental disorders, Tau protein, macromolecular substances, Original Research, crosstalk
Abstract: The formation of intraneuronal fibrillar inclusions of tau protein is associated with several neurodegenerative diseases referred to as tauopathies including Alzheimer's disease (AD). A common feature of these pathologies is hyperphosphorylation of tau, the main component of fibrillar assemblies such as Paired Helical Filaments (PHFs). O-β-linked N-acetylglucosaminylation (O-GlcNAcylation) is another important posttranslational modification involved in regulation of tau pathophysiology. Among the benefits of O-GlcNAcylation, modulation of tau phosphorylation levels and inhibition of tau aggregation properties have been described while decreased O-GlcNAcylation could be involved in the raise of tau phosphorylation associated with AD. However, the molecular mechanisms at the basis of these observations remain to be defined. In this study, we identify by NMR spectroscopy O-GlcNAc sites in the longest isoform of tau and investigate the direct role of O-GlcNAcylation on tau phosphorylation and conversely, the role of phosphorylation on tau O-GlcNAcylation. We show here by a systematic examination of the quantitative modification patterns by NMR spectroscopy that O-GlcNAcylation does not modify phosphorylation of tau by the kinase activity of ERK2 or a rat brain extract while phosphorylation slightly increases tau O-GlcNAcylation by OGT. Our data suggest that indirect mechanisms act in the reciprocal regulation of tau phosphorylation and O-GlcNAcylation in vivo involving regulation of the enzymes responsible of phosphate and O-GlcNAc dynamics.
Published: 2018

32. Interaction study between HCV NS5A-D2 and NS5B using 19F NMR

Author: Guy Lippens, Xavier Hanoulle, Marie Dujardin, François-Xavier Cantrelle, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), ANR-11-JSV8-005, French National Agency for Research on AIDS and Viral Hepatitis [A02014-2], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), ANR-11-JSV8-0005,StruFunc5A5B,Etude des relations structurales et fonctionnelles entre les protéines NS5A et NS5B du virus de l'hépatite C et la Cyclophiline A humaine.(2011), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Stereochemistry, Hepatitis C virus, viruses, [SDV]Life Sciences [q-bio], constante de dissociation, RNA-dependent RNA polymerase, HCV NS5A-D2, Fluorine-19 NMR, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Biochemistry, Protein–protein interaction, protéine non structurale, 03 medical and health sciences, chemistry.chemical_compound, arn, labelling method, medicine, méthode de marquage, Surface plasmon resonance, hepatitis c, NS5A, NS5B, Spectroscopy, réplication, 19F protein labelling, 19F NMR spectroscopy, RNA, virus diseases, biochemical phenomena, metabolism, and nutrition, digestive system diseases, hépatite c, 0104 chemical sciences, 3. Good health, HCV NS5B, 030104 developmental biology, chemistry
Abstract: The non structural protein 5A (NS5A) regulates the replication of the hepatitis C viral RNA through a direct molecular interaction of its domain 2 (NS5A-D2) with the RNA dependent RNA polymerase NS5B. Because of conflicting data in the literature, we study here this molecular interaction using fluorinated versions of the NS5A-D2 protein derived from the JFH1 Hepatitis C Virus strain. Two methods to prepare fluorine-labelled NS5A-D2 involving the biosynthetic incorporation of a F-19-tryptophan using 5-fluoroindole and the posttranslational introduction of fluorine by chemical conjugation of 2-iodo-N-(trifluoromethyl)acetamide with the NS5A-D2 cysteine side chains are presented. The dissociation constants (K-D) between NS5A-D2 and NS5B obtained with these two methods are in good agreement, and yield values comparable to those derived previously from a surface plasmon resonance study. We compare benefits and limitations of both labeling methods to study the interaction between an intrinsically disordered protein and a large molecular target by F-19 NMR.
Published: 2018

33. F2-06-01: MAJOR DIFFERENCES BETWEEN THE SELF-ASSEMBLY, SEEDING BEHAVIOR, AND INTERACTION WITH MODULATORS OF HEPARIN-INDUCED VERSUS IN-VITRO PHOSPHORYLATED TAU

Author: Chunyu Wang, Fuming Zhang, Guy Lippens, Isabelle Huvent, Thomas Schrader, Shiguo Chen, Zizheng Li, Jing Zhao, François-Xavier Cantrelle, Isabelle Landrieu, Robert J. Linhardt, Jianle Chen, Gal Bitan, Jing Di, Caroline Smet-Nocca, Béatrice Chambraud, Frank-Gerrit Klärner, and Clément Despres
Subjects: Epidemiology, Chemistry, Health Policy, Heparin, In vitro, Psychiatry and Mental health, Cellular and Molecular Neuroscience, Developmental Neuroscience, Tau phosphorylation, medicine, Biophysics, Seeding, Neurology (clinical), Geriatrics and Gerontology, medicine.drug
Published: 2019

34. Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7

Author: Zoé Lens, Xavier Hanoulle, Frédérique Dewitte, François-Xavier Cantrelle, Riccardo Peruzzini, Isabelle Landrieu, Marc Aumercier, Elisabeth Ferreira, Jean-Luc Baert, Vincent Villeret, Didier Monté, Alexis Verger, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), The NMR facilities were funded by the Hauts-de-France Regional Council, CNRS, Pasteur Institute of Lille, European Community (FEDER), French Research Ministry and the University of Sciences and Technologies of Lille I. We acknowledge support from the TGE RMN THC (FR-3050, France), FRABio (FR-3688, France), 'Projets Emergents' Nord-Pas-de-Calais Regional Council and Lille NMR and RPE Health and Biology core facility. R.P. was funded by a fellowship from Nord-Pas-de-Calais Regional Council., The FP7 WeNMR (project no. 261572) and H2020 West-Life (project no. 675858) European e-Infrastructure projects are acknowledged for the use of their Web portals., Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE12-0021,MEDNET,Le complexe Médiateur: d'un réseau d'interactions protéine-protéine à la régulation génique in vivo(2016), and Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Magnetic Resonance Spectroscopy, Protein Conformation, Protein subunit, RNA polymerase II, Biology, MESH: Transcription Factor TFIID/metabolism, 03 medical and health sciences, chemistry.chemical_compound, NMR spectroscopy, MESH: Protein Conformation, Structural Biology, Transcription (biology), RNA polymerase, Protein Interaction Mapping, Humans, MESH: Mediator Complex/metabolism, MESH: Protein Binding, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, protein structure, Molecular Biology, TATA-Binding Protein Associated Factors, MESH: Transcription Factors/metabolism, MESH: Humans, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], MEDiator complex, MESH: Magnetic Resonance Spectroscopy, MESH: Protein Interaction Mapping, MED26, MESH: Transcription Factor TFIID/chemistry, TAF7, Dissociation constant, Crystallography, MESH: Mediator Complex/chemistry, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 030104 developmental biology, protein–protein interaction, chemistry, MESH: TATA-Binding Protein Associated Factors/chemistry, MESH: TATA-Binding Protein Associated Factors/metabolism, Helix, biology.protein, Biophysics, Transcription Factor TFIID, transcription regulation, Protein Binding, Transcription Factors
Abstract: International audience; MED26 is a subunit of Mediator, a large complex central to the regulation of gene transcription by RNA Polymerase II. MED26 plays a role in the switch between the initiation and elongation phases of RNA Polymerase II-mediated transcription process. Regulation of these steps requires successive binding of MED26 N-terminal domain (NTD) to TATA-binding protein-associated factor 7 (TAF7) and Eleven-nineteen lysine-rich in leukemia-Associated Factor 1 (EAF1). In order to investigate the mechanism of regulation by MED26, MED26-NTD structure was solved by NMR, revealing a 4-helix bundle. EAF1 (239-268) and TAF7 (205-235) peptide interactions were both mapped to the same groove formed by H3 and H4 helices of MED26-NTD. Both interactions are characterized by dissociation constants in the 10-μM range. Further experiments revealed a folding-upon-binding mechanism that leads to the formation of EAF1 (N247-S260) and TAF7 (L214-S227) helices. Chemical shift perturbations and nuclear Overhauser enhancement contacts support the involvement of residues I222/F223 in anchoring TAF7 helix to a hydrophobic pocket of MED26-NTD, including residues L48, W80 and I84. In addition, Ala mutations of charged residues located in the C-terminal disordered part of TAF7 and EAF1 peptides affected the binding, with a loss of affinity characterized by a 10-time increase of dissociation constants. A structural model of MED26-NTD/TAF7 complex shows bi-partite components, combining ordered and disordered segments, as well as hydrophobic and electrostatic contributions to the binding. This study provides molecular detail that will help to decipher the mechanistic basis for the initiation to elongation switch-function mediated by MED26-NTD.
Published: 2017

35. Regulation of the interaction between the neuronal BIN1 isoform 1 and Tau proteins - role of the SH3 domain

Author: Isabelle Landrieu, François-Xavier Cantrelle, Yoann Sottejeau, Guy Lippens, Idir Malki, Jean-Charles Lambert, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), The NMR facilities were funded by the Nord Region Council, CNRS, Pasteur Institute of Lille, European Community (FEDER), French Research Ministry and Lille University. We acknowledge support from TGE RMN THC (FR-3050, France) and FRABio (Univ. Lille, CNRS, FR 3688). This study was supported by a grant from the LabEx (Laboratory of Excellence), DISTALZ (Development of Innovative Strategies for a Transdisciplinary approach to Alzheimer's disease), by the French government funding agency Agence Nationale de la Recherche ANR-15-CE16-0002 BinAlz and by the Alzheimer's association (BFG-14-318355)., Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), CNRS, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE], and Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Subjects: 0301 basic medicine, Models, Molecular, Protein Conformation, alpha-Helical, Alzheimer’s disease, BIN1, nuclearmagnetic resonance spectroscopy, protein–protein interaction, SH3 domain, Tau, MESH: Sequence Homology, Amino Acid, MESH: Adaptor Proteins, Signal Transducing/metabolism, Amino Acid Motifs, MESH: Tumor Suppressor Proteins/genetics, Gene Expression, Peptide, Biochemistry, MESH: Amino Acid Motifs, 0302 clinical medicine, MESH: Adaptor Proteins, Signal Transducing/genetics, MESH: Nuclear Magnetic Resonance, Biomolecular, Protein Isoforms, Cloning, Molecular, MESH: tau Proteins/chemistry, chemistry.chemical_classification, Neurons, biology, MESH: Kinetics, Chemistry, Nuclear Proteins, MESH: Neurons/metabolism, Nuclear magnetic resonance spectroscopy, MESH: Peptides/genetics, MESH: Tumor Suppressor Proteins/chemistry, MESH: tau Proteins/metabolism, Recombinant Proteins, MESH: Tumor Suppressor Proteins/metabolism, MESH: Recombinant Proteins/chemistry, MESH: tau Proteins/genetics, MESH: Peptides/metabolism, Domain (ring theory), MESH: Protein Conformation, beta-Strand, MESH: Protein Isoforms/metabolism, MESH: Models, Molecular, MESH: Nuclear Proteins/genetics, Protein Binding, Gene isoform, MESH: Recombinant Proteins/metabolism, MESH: Gene Expression, MESH: Sequence Alignment, tau Proteins, MESH: Escherichia coli/genetics, Clathrin, Protein–protein interaction, MESH: Peptides/chemistry, 03 medical and health sciences, Escherichia coli, Humans, MESH: Protein Binding, Protein Interaction Domains and Motifs, MESH: Adaptor Proteins, Signal Transducing/chemistry, MESH: Recombinant Proteins/genetics, MESH: Cloning, Molecular, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Nuclear Proteins/metabolism, Molecular Biology, Gene, Nuclear Magnetic Resonance, Biomolecular, Adaptor Proteins, Signal Transducing, MESH: Protein Conformation, alpha-Helical, MESH: Protein Interaction Domains and Motifs, Binding Sites, MESH: Protein Isoforms/genetics, MESH: Humans, Sequence Homology, Amino Acid, Tumor Suppressor Proteins, Cell Biology, MESH: Nuclear Proteins/chemistry, Kinetics, MESH: Neurons/chemistry, 030104 developmental biology, MESH: Binding Sites, MESH: Protein Isoforms/chemistry, Biophysics, biology.protein, MESH: Escherichia coli/metabolism, Protein Conformation, beta-Strand, Peptides, Sequence Alignment, 030217 neurology & neurosurgery
Abstract: International audience; Bridging integrator 1 (bin1) gene is a genetic determinant of Alzheimer's disease (AD) and has been reported to modulate Alzheimer's pathogenesis through pathway(s) involving Tau. The functional impact of Tau/BIN1 interaction as well as the molecular details of this interaction are still not fully resolved. As a consequence, how BIN1 through its interaction with Tau affects AD risk is also still not determined. To progress in this understanding, interaction of Tau with two BIN1 isoforms was investigated using Nuclear Magnetic Resonance spectroscopy. 1H, 15N spectra showed that the C-terminal SH3 domain of BIN1 isoform 1 (BIN1Iso1) is not mobile in solution but locked with the core of the protein. In contrast, the SH3 domain of BIN1 isoform 9 (BIN1Iso9) behaves as an independent mobile domain. This reveals an equilibrium between close and open conformations for the SH3 domain. Interestingly, a 334–376 peptide from the clathrin and AP-2-binding domain (CLAP) domain of BIN1Iso1, which contains a SH3-binding site, is able to compete with BIN1-SH3 intramolecular interaction. For both BIN1 isoforms, the SH3 domain can interact with Tau(210–240) sequence. Tau(210–240) peptide can indeed displace the intramolecular interaction of the BIN1-SH3 of BIN1Iso1 and form a complex with the released domain. The measured Kd were in agreement with a stronger affinity of Tau peptide. Both CLAP and Tau peptides occupied the same surface on the BIN1-SH3 domain, showing that their interaction is mutually exclusive. These results emphasize an additional level of complexity in the regulation of the interaction between BIN1 and Tau dependent of the BIN1 isoforms.
Published: 2017

36. Identification of the Tau phosphorylation pattern that drives its aggregation

Author: François-Xavier Cantrelle, Haoling Qi, Yves Jacquot, Guy Lippens, Isabelle Huvent, Isabelle Landrieu, Cillian Byrne, Clément Despres, Béatrice Chambraud, Etienne-Emile Baulieu, Caroline Smet-Nocca, CNRS, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Laboratoire des biomolécules [LBM UMR 7203], Université Pierre et Marie Curie - Paris 6 [UPMC], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Petites Molécules de neuroprotection, neurorégénération et remyélinisation, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Neuroprotection et neurogénération (UMR788), Neuroprotection et Neurodégénération (UMR788), TGE RMN THC (Tres Grands Equipements Resonance Magnetique Nucleaire Tres Hauts Champs, France) [FR-3050], FRABio (Federation de Recherche 'Biochimie Structurale & Fonctionnelle des Assemblages Biomoleculaires', France [FR 3688], MetaToul [MetaboHUB-AR-11-INBS-0010], Université de Lille-Centre National de la Recherche Scientifique (CNRS), Landrieu, Isabelle, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), and Lippens, Guy
Subjects: 0301 basic medicine, Models, Molecular, Threonine, inorganic chemicals, Phosphorylation sites, Magnetic Resonance Spectroscopy, Tau, phosphorylation, aggregation, Alzheimer's disease, NMR, tau Proteins, Biotechnologies, macromolecular substances, Protein Aggregation, Pathological, environment and public health, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Microscopy, Electron, Transmission, Protein Domains, Alzheimer’s disease, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Serine, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, ComputingMilieux_MISCELLANEOUS, Multidisciplinary, Binding Sites, Kinase, Nuclear magnetic resonance spectroscopy, Biological Sciences, Fluorescence, In vitro, Peptide Fragments, 3. Good health, enzymes and coenzymes (carbohydrates), 030104 developmental biology, chemistry, Biochemistry, Tau phosphorylation, Phosphorylation, bacteria, Thioflavin, 030217 neurology & neurosurgery
Abstract: International audience; Determining the functional relationship between Tau phosphorylation and aggregation has proven a challenge owing to the multiple potential phosphorylation sites and their clustering in the Tau sequence. We use here in vitro kinase assays combined with NMR spectroscopy as an analytical tool to generate well-characterized phosphorylated Tau samples and show that the combined phosphorylation at the Ser202/Thr205/Ser208 sites, together with absence of phosphorylation at the Ser262 site, yields a Tau sample that readily forms fibers, as observed by thioflavin T fluorescence and electron microscopy. On the basis of conformational analysis of synthetic phosphorylated peptides, we show that aggregation of the samples correlates with destabilization of the turn-like structure defined by phosphorylation of Ser202/Thr205.
Published: 2017

37. Interaction study between HCV NS5A-D2 and NS5B using

Author: Marie, Dujardin, François-Xavier, Cantrelle, Guy, Lippens, and Xavier, Hanoulle
Subjects: Fluorine-19 Magnetic Resonance Imaging, Isotope Labeling, Fluorine, Hepacivirus, Viral Nonstructural Proteins, Nuclear Magnetic Resonance, Biomolecular
Abstract: The non structural protein 5A (NS5A) regulates the replication of the hepatitis C viral RNA through a direct molecular interaction of its domain 2 (NS5A-D2) with the RNA dependent RNA polymerase NS5B. Because of conflicting data in the literature, we study here this molecular interaction using fluorinated versions of the NS5A-D2 protein derived from the JFH1 Hepatitis C Virus strain. Two methods to prepare fluorine-labelled NS5A-D2 involving the biosynthetic incorporation of a
Published: 2017

38. The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein

Author: Jeremy Gunawardena, Caroline Smet-Nocca, Isabelle Landrieu, François-Xavier Cantrelle, Haoling Qi, Béatrice Chambraud, Clément Despres, Guy Lippens, Sudhakaran Prabakaran, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Harvard Medical School [Boston] (HMS), Petites Molécules de neuroprotection, neurorégénération et remyélinisation, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nocca Smet, Caroline, ANR-11-LABX-0009,DISTALZ,Développement de stratégies innovantes pour une approche transdisciplinaire de la maladie d'Alzheime(2011), and Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Recombinant proteins, biology, Kinase, Chemistry, Tau protein, Creb-binding protein, Acetylation, Nuclear magnetic resonance spectroscopy, ERK kinase, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, NMR spectroscopy, Biochemistry, Acetyltransferase, biology.protein, Phosphorylation, Kinase activity, CREB-binding protein, 030217 neurology & neurosurgery
Abstract: Nuclear magnetic resonance (NMR) spectroscopy can be used as an analytical tool to investigate posttranslational modifications of protein. NMR is a valuable tool to map the interaction regions of protein partners. Here, we present protocols that have been developed in the course of our studies of the neuronal Tau protein. Tau is found aggregated in the neurons of Alzheimer’s disease patients. Development of the disease is accompanied by increased, abnormal phosphorylation and acetylation of Tau. We have used NMR to investigate how these posttranslational modifications of Tau affect the interactions with its partners. We present here detailed protocols of in vitro phosphorylation of Tau by recombinant kinase, ERK2, or kinase activity of rat brain extracts, and acetylation by recombinant Creb-binding protein (CBP) acetyltransferase. The analytical characterization of the modified Tau by NMR spectroscopy is additionally described.
Published: 2017

39. NMR reveals the intrinsically disordered domain 2 of NS5A protein as an allosteric regulator of the hepatitis C virus RNA polymerase NS5B

Author: Isabelle Landrieu, Robert Schneider, Guy Lippens, Hélène Launay, Xavier Hanoulle, Luiza M. Bessa, François-Xavier Cantrelle, Marie Dujardin, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), CNRS (IR RMN THC) [FR-3050], ANR-11-JSV8-005, CNRS, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Université de Lille-Centre National de la Recherche Scientifique (CNRS), Schneider, Robert, and Hanoulle, Xavier
Subjects: Models, Molecular, 0301 basic medicine, RNA-protein interaction, Protein Conformation, viruses, Hepacivirus, Viral Nonstructural Proteins, Biochemistry, Protein Refolding, protein-protein interaction, chemistry.chemical_compound, RNA-Protein Interaction, RNA polymerase, chimiothérapie, Enzyme Inhibitors, Polymerase, ComputingMilieux_MISCELLANEOUS, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], virus diseases, Molecular Bases of Disease, hépatite c, Recombinant Proteins, 3. Good health, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Allosteric Site, nuclear magnetic resonance (NMR), Allosteric regulation, Médecine humaine et pathologie, Antiviral Agents, arn, 03 medical and health sciences, Point Mutation, Protein Interaction Domains and Motifs, Isoleucine, NS5A, Nuclear Magnetic Resonance, Biomolecular, Molecular Biology, NS5B, Oligoribonucleotides, génome, RNA, Cell Biology, Triazoles, biochemical phenomena, metabolism, and nutrition, RNA-Dependent RNA Polymerase, Peptide Fragments, digestive system diseases, allosteric regulation, Intrinsically Disordered Proteins, 030104 developmental biology, Solubility, Allosteric enzyme, chemistry, Pyrones, protein dynamic, Mutagenesis, Site-Directed, biology.protein, Biophysics, Human health and pathology, Gene Deletion
Abstract: International audience; Non-structural protein 5B (NS5B) is the RNA-dependent RNA polymerase that catalyzes replication of the hepatitis C virus (HCV) RNA genome and therefore is central for its life cycle. NS5B interacts with the intrinsically disordered domain 2 of NS5A (NS5A-D2), another essential multifunctional HCV protein that is required for RNA replication. As a result, these two proteins represent important targets for anti-HCV chemotherapies. Despite this importance and the existence of NS5B crystal structures, our understanding of the conformational and dynamic behavior of NS5B in solution and its relationship with NS5A-D2 remains incomplete. To address these points, we report the first detailed NMR spectroscopic study of HCV NS5B lacking its membrane anchor (NS5B(21)). Analysis of constructs with selective isotope labeling of the 1 methyl groups of isoleucine side chains demonstrates that, in solution, NS5B(21) is highly dynamic but predominantly adopts a closed conformation. The addition of NS5A-D2 leads to spectral changes indicative of binding to both allosteric thumb sites I and II of NS5B(21) and induces long-range perturbations that affect the RNA-binding properties of the polymerase. We compared these modifications with the short- and long-range effects triggered in NS5B(21) upon binding of filibuvir, an allosteric inhibitor. We demonstrate that filibuvir-bound NS5B(21) is strongly impaired in the binding of both NS5A-D2 and RNA. NS5A-D2 induces conformational and functional perturbations in NS5B similar to those triggered by filibuvir. Thus, our work highlights NS5A-D2 as an allosteric regulator of the HCV polymerase and provides new insight into the dynamics of NS5B in solution.
Published: 2017

40. A functional fragment of Tau forms fibers without the need for an intermolecular cysteine bridge

Author: Amina Kamah, Isabelle Landrieu, Isabelle Huvent, Guy Lippens, Christian Slomianny, François-Xavier Cantrelle, Nicolas Barois, Caroline Smet-Nocca, Université Lille Nord de France (COMUE), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Plateforme BioImaging Center Lille (BICeL), Plateformes Lilloises en Biologie et Santé - UMS 2014 - US 41 (PLBS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie Cellulaire : Canaux ioniques, inflammation et cancer - U 1003 (PHYCELL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Lille, Plate-forme BICeL (IFR142), Physiologie Cellulaire (PHYCEL) - U1003 (PHYCEL), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE), Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 (PLBS)
Subjects: Magnetic Resonance Spectroscopy, Biophysics, tau Proteins, macromolecular substances, Fibril, Biochemistry, law.invention, Aggregation, 03 medical and health sciences, chemistry.chemical_compound, NMR spectroscopy, 0302 clinical medicine, law, Microtubule, mental disorders, Electron microscopy, Humans, Cysteine, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Binding site, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Heparin, Cell Biology, Nuclear magnetic resonance spectroscopy, Fluorescence, Recombinant Proteins, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Crystallography, nervous system, chemistry, Nucleation, Thioflavin, Tau, Electron microscope, 030217 neurology & neurosurgery
Abstract: International audience; We study the aggregation of a fragment of the neuronal protein Tau that contains part of the proline rich domain and of the microtubule binding repeats. When incubated at 37 °C with heparin, the fragment readily forms fibers as witnessed by Thioflavin T fluorescence. Electron microscopy and NMR spectroscopy show bundled ribbon like structures with most residues rigidly incorporated in the fibril. Without its cysteines, this fragment still forms fibers of a similar morphology, but with lesser Thioflavin T binding sites and more mobility for the C-terminal residues.
Published: 2014

41. The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein

Author: Haoling, Qi, Clément, Despres, Sudhakaran, Prabakaran, François-Xavier, Cantrelle, Béatrice, Chambraud, Jeremy, Gunawardena, Guy, Lippens, Caroline, Smet-Nocca, and Isabelle, Landrieu
Subjects: Mitogen-Activated Protein Kinase 1, Neurons, Alzheimer Disease, Animals, Acetylation, tau Proteins, Phosphorylation, CREB-Binding Protein, Nuclear Magnetic Resonance, Biomolecular, Protein Processing, Post-Translational, Recombinant Proteins, Rats
Abstract: Nuclear magnetic resonance (NMR) spectroscopy can be used as an analytical tool to investigate posttranslational modifications of protein. NMR is a valuable tool to map the interaction regions of protein partners. Here, we present protocols that have been developed in the course of our studies of the neuronal Tau protein. Tau is found aggregated in the neurons of Alzheimer's disease patients. Development of the disease is accompanied by increased, abnormal phosphorylation and acetylation of Tau. We have used NMR to investigate how these posttranslational modifications of Tau affect the interactions with its partners. We present here detailed protocols of in vitro phosphorylation of Tau by recombinant kinase, ERK2, or kinase activity of rat brain extracts, and acetylation by recombinant Creb-binding protein (CBP) acetyltransferase. The analytical characterization of the modified Tau by NMR spectroscopy is additionally described.
Published: 2016

42. Nanobodies (VHHs) for targeting tau in Alzheimer's disease and tauopathies

Author: Morvane Colin, François-Xavier Cantrelle, Xavier Hanoulle, Elian Dupre, Alexis Arrial, Clément Danis, Jean-Christophe Rain, Luc Buée, and Isabelle Landrieu
Subjects: business.industry, General Neuroscience, Medicine, Disease, business, Neuroscience
Published: 2019

43. Investigation of Homeodomain Membrane Translocation Properties: Insights from the Structure Determination of Engrailed-2 Homeodomain in Aqueous and Membrane-Mimetic Environments

Author: Olivier Lequin, Alain Joliot, Lucie Khemtémourian, Stéphane Balayssac, Ludovic Carlier, François-Xavier Cantrelle, Gérard Chassaing, Université Pierre et Marie Curie - Paris 6 (UPMC), Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (SPCMIB), Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Synthèse, Structure et Fonction de Molécules Bioactives (SSFMB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Conformational change, Circular dichroism, media_common.quotation_subject, Molecular Sequence Data, Biophysics, Nerve Tissue Proteins, Biology, 010402 general chemistry, 01 natural sciences, Micelle, 03 medical and health sciences, Animals, Humans, Amino Acid Sequence, Internalization, Micelles, 030304 developmental biology, media_common, Homeodomain Proteins, 0303 health sciences, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Cell Membrane, Membrane, Biological membrane, engrailed, 0104 chemical sciences, Protein Structure, Tertiary, Crystallography, Protein Transport, Helix, Drosophila, Chickens
Abstract: International audience; In addition to their well-known DNA-binding properties, homeodomains have the ability to efficiently translocate across biological membranes through still poorly-characterized mechanisms. To date, most biophysical studies addressing the mechanisms of internalization have focused on small synthetic peptides rather than full-length globular homeodomains. In this work, we characterized the conformational properties of chicken Engrailed 2 homeodomain (En2HD) in aqueous solution and in membrane mimetic environments using circular dichroism, Trp fluorescence, and NMR spectroscopy. En2HD adopts a well-defined three-helical bundle fold in aqueous solution. The Trp-48 residue, which is critical for internalization, is fully buried in the hydrophobic core. Circular dichroism and fluorescence reveal that a conformational transition occurs in anionic lipid vesicles and in micelles. En2HD loses its native three-dimensional structure in micellar environments but, remarkably, near-native helical secondary structures are maintained. Long-range interactions could be detected using site-directed spin labels, indicating that the three helices do not adopt extended orientations. Noncovalent paramagnetic probes yielded information about helix positioning and unveiled the burial of critical aromatic and basic residues within the micelles. Our results suggest that electrostatic interactions with membranes may be determinant in inducing a conformational change enabling Trp-48 to insert into membranes.
Published: 2013
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44. A β-Turn Motif in the Steroid Hormone Receptor’s Ligand-Binding Domains Interacts with the Peptidyl-prolyl Isomerase (PPIase) Catalytic Site of the Immunophilin FKBP52

Author: François-Xavier Cantrelle, Guy Lippens, Morkos A. Henen, Haoling Qi, Julien Giustiniani, Amina Kamah, Yves Jacquot, Isabelle Landrieu, Mathilde Belnou, Etienne-Emile Baulieu, Cillian Byrne, Béatrice Chambraud, Université Pierre et Marie Curie - Paris 6 (UPMC), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Neuroprotection et neurogénération (UMR788), Neuroprotection et Neurodégénération (UMR788), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Region Nord, Institut Pasteur of Lille, European Union (FEDER), French Research Ministry, Universite des Sciences et Technologies de Lille 1, University Pierre & Marie Curie (Paris 6) [ED406], Centre National de la Recherche Scientifique (CNRS), Ecole Normale Superieure (ENS), Institut Baulieu, Alanna Schepartz, European Project: 286418,EC:FP7:PEOPLE,FP7-PEOPLE-2011-IAPP,14-3-3STABS(2012), and Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Steroid hormone receptor, Stereochemistry, medicine.medical_treatment, Receptors, Cytoplasmic and Nuclear, Isomerase, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Biology, Ligands, 010402 general chemistry, 01 natural sciences, Biochemistry, 03 medical and health sciences, Catalytic Domain, Prolyl isomerase, medicine, Binding site, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Receptor, Nuclear Magnetic Resonance, Biomolecular, ComputingMilieux_MISCELLANEOUS, Binding Sites, Peptidylprolyl Isomerase, FKBP52, 0104 chemical sciences, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Steroid hormone, 030104 developmental biology, Thermodynamics, Steroids, FKBP1A, Protein Binding
Abstract: International audience; The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor alpha (hER alpha) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II beta-turn conformation in the protein, binds the peptidyl-prolyl isomerase (PPIase or rotamase) FK1 domain of FKBP52. Interestingly, this turn motif displays strong similarities with the FKBP52 FK1 domain-binding moiety of macrolide immunomodulators such as rapamycin and GPI-1046, an immunophilin ligand with neuroprotective characteristics. An increase in the hydrophobicity of the residue preceding the proline and cyclization of the VPGF peptide strengthen its recognition by the FK1 domain of FKBP52. Replacement of the Pro residue with a dimethylproline also enhances this interaction. Our study not only contributes to a better understanding of how the interaction between the FK1 domain of FKBP52 and steroid hormone receptors most likely works but also opens new avenues for the synthesis of FKBP52 FK1 peptide ligands appropriate for the control of hormone-dependent physiological mechanisms or of the functioning of the Tau protein. Indeed, it has been shown that FKBP52 is involved in the intraneuronal dynamics of the Tau protein.
Published: 2016

45. Kinetically Controlled Chemoselective Cyclization Simplifies the Access to Cyclic and Branched Peptides

Author: Oleg Melnyk, Hervé Drobecq, François-Xavier Cantrelle, Elizabeth Lissy, Emmanuelle Boll, CBC, Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé-Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, IFR 142 (IFR 142), Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Subjects: chemistry.chemical_classification, 010405 organic chemistry, Stereochemistry, Chemistry, Organic Chemistry, Peptide, 010402 general chemistry, 01 natural sciences, Biochemistry, Combinatorial chemistry, 0104 chemical sciences, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Group (periodic table), [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, ComputingMilieux_MISCELLANEOUS
Abstract: A bis(2-sulfanylethyl)amido group reacts significantly faster with cysteinyl peptides when installed on the C-terminal end of a peptide in comparison with the side-chain of Asp and Glu. This property enabled the design of a kinetically controlled chemoselective peptide cyclization reaction, giving straightforward access to cyclic and branched peptides in one pot.
Published: 2016

46. Characterization of Neuronal Tau Protein as a Target of Extracellular Signal-regulated Kinase

Author: Haoling Qi, Jeremy Gunawardena, Guy Lippens, Béatrice Chambraud, François-Xavier Cantrelle, Sudhakaran Prabakaran, Isabelle Landrieu, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Department of Systems Biology, Harvard Medical School [Boston] (HMS), Neuroprotection et neurogénération (UMR788), Neuroprotection et Neurodégénération (UMR788), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), CNRS, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Harvard Medical School [Boston] [HMS], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Petites Molécules de neuroprotection, neurorégénération et remyélinisation, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Landrieu, Isabelle, Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Prabakaran, Sudhakaran [0000-0002-6527-1085], and Apollo - University of Cambridge Repository
Subjects: 0301 basic medicine, MAPK/ERK pathway, Amino Acid Motifs, spectroscopie rmn, Biochemistry, environment and public health, protein-protein interaction, neurodégénérescence, Protein phosphorylation, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Kinase, Neurodegeneration, Molecular Bases of Disease, 3. Good health, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], maladie d'alzheimer, extracellular signal-regulated kinase (ERK), protéine, cerveau, Tau protein (Tau), Alzheimer's disease, Alzheimer disease, inorganic chemicals, nuclear magnetic resonance (NMR), Tau protein, Médecine humaine et pathologie, Hyperphosphorylation, tau Proteins, Biology, 03 medical and health sciences, interaction Tau protein (Tau), mental disorders, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, protein phosphorylation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], protein phosphorylation protein-protein, Nuclear Magnetic Resonance, Biomolecular, Molecular Biology, Cell Biology, medicine.disease, Protein Structure, Tertiary, Rats, enzymes and coenzymes (carbohydrates), 030104 developmental biology, biology.protein, Human health and pathology
Abstract: International audience; Tau neuronal protein has a central role in neurodegeneration and is implicated in Alzheimer disease development. Abnormal phosphorylation of Tau impairs its interaction with other proteins and is associated with its dysregulation in pathological conditions. Molecular mechanisms leading to hyperphosphorylation of Tau in pathological conditions are unknown. Here, we characterize phosphorylation of Tau by extracellular-regulated kinase (ERK2), a mitogen-activated kinase (MAPK) that responds to extracellular signals. Analysis of in vitro phosphorylated Tau by activated recombinant ERK2 with nuclear magnetic resonance spectroscopy (NMR) reveals phosphorylation of 15 Ser/Thr sites. In vitro phosphorylation of Tau using rat brain extract and subsequent NMR analysis identifies the same sites. Phosphorylation with rat brain extract is known to transform Tau into an Alzheimer disease-like state. Our results indicate that phosphorylation of Tau by ERK2 alone is sufficient to produce the same characteristics. We further investigate the mechanism of ERK2 phosphorylation of Tau. Kinases are known to recognize their protein substrates not only by their specificity for a targeted Ser or Thr phosphorylation site but also by binding to linear-peptide motifs called docking sites. We identify two main ERK2 docking sites in Tau sequence using NMR. Our results suggest that ERK2 dysregulation in Alzheimer disease could lead to abnormal phosphorylation of Tau resulting in the pathology of the disease.
Published: 2016

47. 1H, 15N and 13C assignments of the N-terminal domain of the Mediator complex subunit MED26

Author: Isabelle Landrieu, Frédérique Dewitte, Alexis Verger, Riccardo Peruzzini, Elisabeth Ferreira, Vincent Villeret, François-Xavier Cantrelle, Zoé Lens, Jean-Luc Baert, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, congenital, hereditary, and neonatal diseases and abnormalities, Stereochemistry, Protein subunit, RNA polymerase II, 010402 general chemistry, 01 natural sciences, Biochemistry, Protein Structure, Secondary, Protein–protein interaction, 03 medical and health sciences, Mediator, Protein Domains, Structural Biology, Transcription (biology), Transcriptional regulation, Amino Acid Sequence, Nuclear Magnetic Resonance, Biomolecular, ComputingMilieux_MISCELLANEOUS, Genetics, Mediator Complex, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], MED26, Nuclear magnetic resonance spectroscopy, 0104 chemical sciences, Protein Subunits, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 030104 developmental biology, biology.protein
Abstract: MED26 is a subunit of the Mediator, a very large complex involved in regulation of gene transcription by RNA Polymerase II. MED26 regulates the switch between initiation and elongation phases of the transcription. This function requires interaction of its N-terminal domain (NTD) with several protein partners implicated in transcriptional regulation. Molecular details of the structure and interaction mode of MED26 NTD would improve understanding of this complex regulation. As a first step towards structural characterization, sequence specific (1)H, (13)C and (15)N assignments for MED26 NTD was performed based on Nuclear Magnetic Resonance spectroscopy. TALOS+ analysis of the chemical shifts data revealed a domain solely composed of helices. Assignments will be further used to solve NMR structure and dynamics of MED26 NTD and investigate the molecular details of its interaction with protein partners.
Published: 2016

48. Isomerisation and oligomerization of truncated and mutated tau forms by FKBP52 are independent processes

Author: Julien Giustiniani, Yves Jacquot, Isabelle Landrieu, Béatrice Chambraud, Guy Lippens, François-Xavier Cantrelle, Caroline Smet, Etienne-Emile Baulieu, Isabelle Huvent, Kevin Guillemeau, Amina Kamah, Cillian Byrne, Université Lille Nord de France (COMUE), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé-Université de Lille, Sciences et Technologies, Neuroprotection et neurogénération (UMR788), Neuroprotection et Neurodégénération (UMR788), Peptides, glycoconjugués et métaux en biologie (LBM-E1), Laboratoire des biomolécules (LBM UMR 7203), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC), ANR (program MALZ-TAF), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Isomerase activity, Tau protein, Mutant, tau Proteins, Peptide, Protein Aggregation, Pathological, Tacrolimus Binding Proteins, 03 medical and health sciences, 0302 clinical medicine, Isomerism, Structural Biology, mental disorders, Humans, Molecular Biology, FK506 binding, chemistry.chemical_classification, biology, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, aggregation, Alzheimer's disease, FKBP52, prolyl cis/trans isomerase activity, 030104 developmental biology, FKBP, Biochemistry, Biophysics, biology.protein, Protein Multimerization, Tau, 030217 neurology & neurosurgery, Heteronuclear single quantum coherence spectroscopy
Abstract: International audience; The aggregation of the neuronal Tau protein is one molecular hallmark of Alzheimer's disease and other related tauopathies, but the precise molecular mechanisms of the aggregation process remain unclear. The FK506 binding protein FKBP52 is able to induce oligomers in the pathogenic Tau P301L mutant and in a truncated form of the wild-type human Tau protein. Here, we investigate whether FKBP52's capacity to induce Tau oligomers depends on its prolyl cis/trans isomerase activity. We find that FKBP52 indeed can isomerize selected prolyl bonds in the different Tau proteins, and that this activity is carried solely by its first FK506 binding domain. Its capacity to oligomerize Tau is however not linked to this PPIase activity. In addition, we identified a novel molecular interaction implying the PHF6 peptide of Tau and the FK1/FK2 domains of FKBP52 independent of FK506 binding; these data point towards a non-catalytic molecular interaction that might govern the effect of FKBP52 on Tau.
Published: 2016

49. Proline Conformation in a Functional Tau Fragment

Author: Puneet Ahuja, Guy Lippens, Caroline Smet, Jean-Michel Wieruszeski, Juan José Marín López, Xavier Hanoulle, Isabelle Landrieu, François-Xavier Cantrelle, Isabelle Huvent, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Lille, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Magnetic Resonance Spectroscopy, Proline, Stereochemistry, Protein Conformation, Tau protein, tau Proteins, Isomerase, 03 medical and health sciences, Protein structure, Structural Biology, Humans, Phosphorylation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology, Conformational isomerism, ComputingMilieux_MISCELLANEOUS, biology, Chemistry, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Folding (chemistry), 030104 developmental biology, biology.protein, Two-dimensional nuclear magnetic resonance spectroscopy, Protein Processing, Post-Translational, Heteronuclear single quantum coherence spectroscopy
Abstract: The conformational state of distinct prolines can determine the folding of a protein but equally other biological processes when coupled to a conformation-sensitive secondary reaction. For the neuronal tau protein, the importance of proline conformation is underscored by its interaction with different prolyl cis/trans isomerases. The proline conformation would gain even further importance after phosphorylation of the preceding residue by various proline-directed kinases. A number of molecular diseases including Alzheimer's disease and traumatic brain injury were thereby recently qualified as "cistauosis", as they would imply a cis conformation for the pThr231-Pro232 prolyl bond. We here investigate by NMR spectroscopy the conformation of all prolines in a functional Tau fragment, Tau[208-324]. Although we can detect and identify some minor conformers in the cis form, we show that all prolines are for over 90% in the trans conformation. Phosphorylation by CDK2/CycA3, which notably leads to complete modification of the Thr231 residue, does not change this conclusion. Our data hence disagree with the notion that specific prolyl bonds in tau would adopt preferentially the cis conformation.
Published: 2016

50. Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Author: Haoling Qi, Robert Schneider, Xavier Hanoulle, Hamida Merzougui, Isabelle Landrieu, François-Xavier Cantrelle, Clément Despres, Clément Danis, Caroline Smet-Nocca, Luiza M. Bessa, Idir Malki, Guy Lippens, Isabelle Huvent, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer (JPArc - U837 Inserm), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université Lille 2 - Faculté de Médecine, CNRS, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 [JPArc], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), and Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille
Subjects: 0301 basic medicine, Magnetic Resonance Spectroscopy, General Chemical Engineering, Tau protein, tau Proteins, Intrinsically disordered proteins, Biochemistry, General Biochemistry, Genetics and Molecular Biology, Isotopic labeling, 03 medical and health sciences, Alzheimer Disease, Protein purification, mental disorders, Triple-resonance nuclear magnetic resonance spectroscopy, Humans, Protein phosphorylation, Phosphorylation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, biology, General Immunology and Microbiology, Chemistry, General Neuroscience, Nuclear magnetic resonance spectroscopy, Recombinant Proteins, 3. Good health, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Intrinsically Disordered Proteins, 030104 developmental biology, biology.protein, Issue 118, nuclear magnetic resonance spectroscopy, isotopic labeling, intrinsically disordered protein, recombinantprotein, protein purification, protein phosphorylation, NMR data acquisition, resonance assignment
Abstract: Aggregates of the neuronal Tau protein are found inside neurons of Alzheimer's disease patients. Development of the disease is accompanied by increased, abnormal phosphorylation of Tau. In the course of the molecular investigation of Tau functions and dysfunctions in the disease, nuclear magnetic resonance (NMR) spectroscopy is used to identify the multiple phosphorylations of Tau. We present here detailed protocols of recombinant production of Tau in bacteria, with isotopic enrichment for NMR studies. Purification steps that take advantage of Tau's heat stability and high isoelectric point are described. The protocol for in vitro phosphorylation of Tau by recombinant activated ERK2 allows for generating multiple phosphorylations. The protein sample is ready for data acquisition at the issue of these steps. The parameter setup to start recording on the spectrometer is considered next. Finally, the strategy to identify phosphorylation sites of modified Tau, based on NMR data, is explained. The benefit of this methodology compared to other techniques used to identify phosphorylation sites, such as immuno-detection or mass spectrometry (MS), is discussed. 118
Published: 2016

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