Additional file 6: of Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Tau phosphorylation precludes the BIN1-Tau interaction in vitro. 2D [1H, 15N] HSQC spectra of 125 μM 15N CDK-phosphorylated Tau-F5[165–245] free in solution (gray) and with a 1 molar amount of GST-BIN1/SH3 (red, superimposed). No CS perturbations or peak broadening were observed - indicating the absence of interaction between BIN1 and Tau-F5. (PDF 67 kb)