190 results on '"Fernando de Ory"'
Search Results
2. Absence of IgG antibodies among high-risk contacts of two confirmed cases of Crimean-Congo haemorrhagic fever in the autonomous region of Madrid (Spain)
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Pello Latasa, Fernando de Ory, José Ramón Arribas, Maria Ángeles Sánchez-Uriz, Ignacio Sanchez-Arcilla, María Ordobás, Anabel Negredo, Elena Trigo, Pilar Delgado, Marco Marzola, Maria Ángeles Lopaz, María Paz Sánchez-Seco, Fernando de la Calle-Prieto, Pilar Ferrera, Elena Rodriguez, Alejandro Martín, Maria Felipa del Cerro, Esther Córdoba, Marta Mora-Rillo, and Maria José Esteban
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Health surveillance ,Infectious disease transmission ,Serology ,Crimean-Congo haemorrhagic fever virus ,Spain ,Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Crimean-Congo haemorrhagic fever (CCHF) is a widely distributed tick-borne disease. In Spain, the disease has emerged as outbreak associated with high-risk exposures. Our goal was to evaluate the prevalence of antibodies against the CCHF virus (CCHFV) in high-risk contacts. A cross-sectional study was conducted. Three hundred eighty-six high-risk contacts were identified comprising family contacts and hospital workers who had attended the cases. Fifty-seven cases with closer exposure were selected. However, forty-nine cases participated in the study. IgG antibodies were detected by immunoenzymatic techniques. All determinations tested negative for anti-CCHFV IgG antibodies. Most of the responders were women (73.5%), and belong to the intensive care department (53.1%). In relation to other possible sources of exposures, 18.4% travelled to countries with CCHF transmission risk. No CCHF positivity was recorded among selected high-risk contacts. This highlights the importance of standard precautions which might have protected healthcare workers and care providers from CCHF infection.
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- 2020
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3. Hantavirus Pulmonary Syndrome in Traveler Returning from Nepal to Spain
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Elena Sulleiro, Maria Luisa Aznar, Núria Serre-Delcor, Fernando Salvador, Adrian Sanchez-Montalvá, Mateu Espasa, Daniel Molina, Fernando de Ory, M. Paz Sanchez-Seco, Israel Molina, Candido Diaz-Lagares, Miguel J. Martinez, Tomàs Pumarola, and Inés Oliveira
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hantavirus ,cardiopulmonary syndrome ,Nepal ,traveler ,India ,Sri Lanka ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Most human hantavirus infections occur in Asia, but some cases have been described in Europe in travelers returning from Asia. We describe a case of hantavirus pulmonary syndrome in a previously healthy traveler occurring shortly after he returned to Spain from Nepal. Serologic tests suggested a Puumala virus–like infection.
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- 2020
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4. New circulation of genotype V of Crimean-Congo haemorrhagic fever virus in humans from Spain.
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Lia Monsalve Arteaga, Juan Luis Muñoz Bellido, Ana Isabel Negredo, Jorge García Criado, Maria Carmen Vieira Lista, Jesús Ángel Sánchez Serrano, María Belén Vicente Santiago, Amparo López Bernús, Fernando de Ory Manchón, María Paz Sánchez Seco, Nuria Leralta, Montserrat Alonso Sardón, Antonio Muro, and Moncef Belhassen-García
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundCrimean-Congo haemorrhagic fever (CCHF) is a widespread tick-borne viral disease caused by the Crimean-Congo haemorrhagic fever virus (CCHFV). CCHFV has been implicated in severe viral haemorrhagic fever outbreaks. During the summer of 2016, the first two cases with genotype III (Africa 3) were reported in Spain. The first objective of our study was to determine the presence of CCHFV among patients with febrile illness during the spring and summer periods in 2017 and 2018. Finally, we perform a phylogenetic analysis to determine the genotype of the virus.MethodologyWe prospectively evaluated patients aged 18 years and older who came to the emergency department at the Salamanca's University Hospital (HUS) with fever. Specific IgM and IgG antibodies against CCHFV by ELISA and one immunofluorescence assay against two different proteins (nucleoprotein and glycoprotein C) was done. Moreover, molecular detection by Real Time PCR was performed in all collected samples. A phylogenetic analysis was carried out to genetically characterize CCHFV detected in this study.Principal findingsA total of 133 patients were selected. The mean age was 67.63 years and 60.9% were male. One-third of the patients presented an acute undifferentiated febrile illness. Three patients had anti-CCHFV IgG antibodies, suggesting a previous infection. One patient had anti-CCHFV IgM antibodies and a confirmatory RT-PCR. Phylogenetic analysis indicated that the virus corresponds to the European genotype V. This patient came to the emergency department at HUS in August 2018 presenting an acute febrile syndrome with thrombopenia and liver impairment.ConclusionsWe describe a new circulation of European genotype V CCHFV in Spain. Moreover, this study suggests that CCHFV is an identifiable cause of febrile illness of unknown origin in Spain. Thus, CCHF could be suspected in patients with fever, liver damage, and/or haemorrhagic disorders, particularly in people with risk activities who present in the spring or summer.
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- 2021
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5. Measles in Vaccinated People: Epidemiology and Challenges in Surveillance and Diagnosis in the Post-Elimination Phase. Spain, 2014–2020
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Noemí López-Perea, Aurora Fernández-García, Juan Emilio Echevarría, Fernando de Ory, Mayte Pérez-Olmeda, and Josefa Masa-Calles
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measles ,surveillance ,elimination ,vaccination ,vaccine failure ,waning immunity ,Microbiology ,QR1-502 - Abstract
The MMR vaccination program was introduced in Spain in 1981. Consistently high vaccination coverage has led to Spain being declared free of endemic measles transmission since 2014. A few imported and import-related cases were reported during the post-elimination phase (2014 to 2020), with very low incidence: three cases per million of inhabitants a year, 70% in adults. In the post-elimination phase an increasing proportion of measles appeared in two-dose vaccinated individuals (up to 14%), posing a challenge to surveillance and laboratory investigations. Severity and clinical presentation were milder among the vaccinated. The IgM response varied and the viral load decreased, making the virus more difficult to detect. A valid set of samples (serum, urine and throat swab) is strongly recommended for accurate case classification. One third of measles in fully vaccinated people was contracted in healthcare settings, mainly in doctors and nurses, consistent with the important role of high intensity exposure in measles breakthrough cases. Surveillance protocols and laboratory algorithms should be adapted in advanced elimination settings. Reinforcing the immunity of people working in high exposure environments, such as healthcare settings, and implementing additional infection control measures, such as masking and social distancing, are becoming crucial for the global aim of measles eradication.
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- 2021
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6. La infección por virus Zika: una nueva emergencia de salud pública con gran impacto mediático
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Joan A. Caylà, Ángela Domínguez, Elena Rodríguez Valín, Fernando de Ory, Ana Vázquez, and Claudia Fortuny
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Public aspects of medicine ,RA1-1270 - Abstract
Resumen: La infección por virus Zika (VZ) está afectando intensamente a los países latinoamericanos y se ha convertido en una nueva epidemia mediática. Su posible asociación con microcefalia y síndrome de Guillain-Barré motivó que la Organización Mundial de la Salud (OMS) declarase el 1 de febrero de 2016 que esta epidemia constituye una emergencia de salud pública de importancia internacional. Los datos epidemiológicos muestran una incidencia creciente en países como Brasil y Colombia, y que la epidemia sigue expandiéndose por muchos otros países. Desde enero de 2007 hasta el 27 de abril de 2016, la OMS ha detectado transmisión autóctona en 55 países (en 42 de ellos ha sido el primer brote de Zika), y 1198 microcefalias y otros trastornos neurológicos en Brasil. Así mismo, durante 2015 y 2016, 13 países detectaron un incremento de los casos de síndrome de Guillain-Barré y de confirmación de VZ asociado a este. En relación a las microcefalias y otras graves alteraciones cerebrales en recién nacidos de madres afectadas por VZ, las investigaciones ya evidencian una relación causal. Clínicamente muchos casos son asintomáticos y el diagnóstico ofrece dificultades con otras arbovirosis. El control de vectores en España es prioritario, dada la existencia de Aedes albopictus (mosquito tigre). También se recomienda el diagnóstico precoz, evitar viajes a zonas endémicas, mantener relaciones sexuales protegidas y procurar que la prioridad política, que puede evitar que esta epidemia se convierta en una enfermedad endémica de alta prevalencia, no nos haga olvidar otros problemas de salud. Abstract: Infection with Zika virus (ZV) has become a new epidemic, with great impact on the media, and is having a strong effect in Latin American countries. Its possible association with microcephaly and Guillain-Barré syndrome prompted the World Health Organization (WHO) to declare on 1 February 2016 that this epidemic is a public health emergency of international concern. Epidemiological data show an increasing incidence in countries like Brazil and Colombia, and that the epidemic is still expanding in many other countries. Between January 2007 and 27 April 2016, the WHO detected transmission in 55 countries (in 42 of these, this was the first outbreak of Zika) and 1,198 microcephalies and other neurological disorders in Brazil. Also, during 2015-2016, 13 countries detected an increase in Guillain-Barré syndrome and/or confirmation of ZV associated with Guillain-Barré syndrome. Research has already demonstrated a causal relationship between microcephaly and other serious brain disorders in newborns and ZV infection in the mother. Clinically, many cases are asymptomatic and it can be difficult to distinguish this diagnosis from that of other arboviruses. Vector control in Spain is a priority because of the presence of the Aedes albopictus (tiger mosquito). Early diagnosis is recommended, as is avoiding travel to endemic areas and unprotected sex, and ensuring that the high political profile, which can prevent this epidemic from becoming a high prevalence endemic disease, does not cause us to forget about other health problems. Palabras clave: Zika, Salud pública, Emergencia, Epidemias mediáticas, Vectores, control, Guillain-Barré, Microcefalia, Keywords: Zika, Public health, Emergency, Media epidemics, Vector, control, Guillain-Barré, Microcephaly
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- 2016
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7. BROTES EPIDÉMICOS Y SITUACIONES DE ALERTA SANITARIA DE PROBABLE ETIOLOGÍA VÍRICA ESTUDIADOS EN EL CENTRO NACIONAL DE MICROBIOLOGÍA DURANTE EL PERÍODO 2012-2013
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José Manuel Echevarría Mayo, Ana Avellón Calvo, María Cabrerizo Sanz, Inmaculada Casas Flecha, Juan Emilio Echevarría Mayo, Fernando de Ory Manchón, Anabel Negredo Antón, Francisco Pozo Sánchez, María Paz Sánchez-Seco Fariñas, David Tarragó Asensio, and Gloria Trallero Masó
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Medicine ,Public aspects of medicine ,RA1-1270 - Published
- 2016
8. Zika Virus Screening among Spanish Team Members After 2016 Rio de Janeiro, Brazil, Olympic Games
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Natalia Rodriguez-Valero, Alberto M. Borobia, Mar Lago, Maria Paz Sánchez-Seco, Fernando de Ory, Ana Vázquez, Jose Luis Pérez-Arellano, Cristina Carranza Rodríguez, Miguel J. Martínez, Alicia Capón, Elias Cañas, Joaquin Salas-Coronas, Arkaitz Azcune Galparsoro, and Jose Muñoz
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Zika virus ,viruses ,Spain ,Rio de Janeiro ,Brazil ,Olympic Games ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We evaluated the risk for the Spanish Olympic Team acquiring Zika virus in Rio de Janeiro, Brazil, during 2016. We recruited 117 team members, and all tested negative for Zika virus. Lack of cases in this cohort supports the minimum risk estimates made before the Games.
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- 2017
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9. Clinical Outcomes of a Zika Virus Mother–Child Pair Cohort in Spain
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Antoni Soriano-Arandes, Marie Antoinette Frick, Milagros García López-Hortelano, Elena Sulleiro, Carlota Rodó, María Paz Sánchez-Seco, Marta Cabrera-Lafuente, Anna Suy, María De la Calle, Mar Santos, Eugenia Antolin, María del Carmen Viñuela, María Espiau, Ainara Salazar, Borja Guarch-Ibáñez, Ana Vázquez, Juan Navarro-Morón, José-Tomás Ramos-Amador, Andrea Martin-Nalda, Eva Dueñas, Daniel Blázquez-Gamero, Resurrección Reques-Cosme, Iciar Olabarrieta, Luis Prieto, Fernando De Ory, Claire Thorne, Thomas Byrne, Anthony E. Ades, Elisa Ruiz-Burga, Carlo Giaquinto, María José Mellado-Peña, Alfredo García-Alix, Elena Carreras, and Pere Soler-Palacín
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zika virus ,microcephaly ,congenital infection ,adverse outcome ,arboviruses ,Medicine - Abstract
Background: Zika virus (ZIKV) infection has been associated with congenital microcephaly and other neurodevelopmental abnormalities. There is little published research on the effect of maternal ZIKV infection in a non-endemic European region. We aimed to describe the outcomes of pregnant travelers diagnosed as ZIKV-infected in Spain, and their exposed children. Methods: This prospective observational cohort study of nine referral hospitals enrolled pregnant women (PW) who travelled to endemic areas during their pregnancy or the two previous months, or those whose sexual partners visited endemic areas in the previous 6 months. Infants of ZIKV-infected mothers were followed for about two years. Results: ZIKV infection was diagnosed in 163 PW; 112 (70%) were asymptomatic and 24 (14.7%) were confirmed cases. Among 143 infants, 14 (9.8%) had adverse outcomes during follow-up; three had a congenital Zika syndrome (CZS), and 11 other potential Zika-related outcomes. The overall incidence of CZS was 2.1% (95%CI: 0.4–6.0%), but among infants born to ZIKV-confirmed mothers, this increased to 15.8% (95%CI: 3.4–39.6%). Conclusions: A nearly 10% overall risk of neurologic and hearing adverse outcomes was found in ZIKV-exposed children born to a ZIKV-infected traveler PW. Longer-term follow-up of these children is needed to assess whether there are any later-onset manifestations.
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- 2020
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10. Imported Human West Nile Virus Lineage 2 Infection in Spain: Neurological and Gastrointestinal Complications
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María Velasco, María Paz Sánchez-Seco, Carolina Campelo, Fernando de Ory, Oriol Martin, Laura Herrero, Octavio J. Salmerón Béliz, Teodora Minguito, Mª Carmen Campos, Francisca Molero, Alejandro Algora, and Ana Vázquez
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west nile virus lineage 2 ,human ,spain ,neurological symptoms ,digestive complications ,Microbiology ,QR1-502 - Abstract
We report the first human case of West Nile virus (WNV) lineage 2 infection imported to Spain by a traveler returning from Romania. Serum, cerebrospinal fluid and urine samples were analyzed and West Nile virus infection was identified by PCR and serological tests. The patient developed fever, diarrhea and neurological symptoms, accompanied by mild pancreatitis, described previously in very few cases as a complication of WNV infection and by alithiasic cholecystitis. Viral RNA was detected in urine until 30 days after the onset of symptoms and neutralizing antibodies were detected at very low titers. The phylogenetic analysis in a fragment of the NS5 gene of the virus showed a homology with sequences from WNV lineage 2 belonging to the monophyletic Central/Southern European group.
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- 2020
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11. Evaluation of the LIAISON XL Zika Capture IgM II for the Diagnosis of Zika Virus Infections
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Mayte Pérez-Olmeda, María Paz Sánchez-Seco, Ana Vázquez, Pilar Balfagón, Jesús de la Fuente, María Ángeles Murillo, Teodora Minguito, and Fernando de Ory
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zika virus ,chemiluminescent immunoassay ,enzyme immunoassay ,dengue viruses ,chikungunya virus ,Microbiology ,QR1-502 - Abstract
The aim of this study is to evaluate the performance characteristics of the LIAISON XL Zika Capture IgM II. For this purpose we tested 128 samples obtained from recent infections caused by the Zika (ZIKV; 74 samples), dengue (DENV; 10 samples), chikungunya (CHIK V; 11 samples), rubella (RUBV; 10 samples) and measles (MeV; 10 samples) viruses, as well as human parvovirus B19 (HPVB19; 13 samples). The results of the assay under evaluation are compared with those obtained from an indirect immunofluorescence (IIF) assay, and the discrepancies are resolved by considering other laboratory results (PCR and a plaque-reduction neutralization test). The LIAISON showed excellent sensitivity (100%). The specificity (91.25%) was hampered by some false-positive results in recent dengue virus, chikungunya virus, measles virus and human parvovirus B19 infections. The method evaluated is adequate, but the low specificity makes it necessary to consider the clinical and epidemiological contexts of patients, as well as other laboratory results.
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- 2020
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12. Measles virus genotype D4 strains with non-standard length M-F non-coding region circulated during the major outbreaks of 2011-2012 in Spain.
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Horacio Gil, Aurora Fernández-García, María Mar Mosquera, Judith M Hübschen, Ana M Castellanos, Fernando de Ory, Josefa Masa-Calles, and Juan E Echevarría
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Medicine ,Science - Abstract
In recent decades, vaccination has substantially reduced the number of measles cases to levels close to the elimination stage. However, major measles outbreaks occurred in Europe during 2010-2012, after the introduction of the D4-Enfield lineage. We have performed a molecular characterization of 75 measles virus genotype D4 strains from patients infected in Spain between 2004 and 2012 by sequencing the N-450 region and the M-F non-coding region (M-F NCR) in order to identify genetic features of these viruses. The analysis of the N-450 region confirmed that all samples obtained since 2008 belonged to variants or sets of identical sequences of the D4-Enfield lineage, including a new one named MVs/Madrid.ESP/46.10/. Analysis of the M-F NCR showed insertions and deletions associated with previously described, uncommon non-standard genome length measles viruses. This genetic feature was identified in the D4-Enfield lineage viruses, but not in the other D4 viruses that were circulating in Spain before 2008, suggesting that these non-standard length M-F NCR sequences are characteristic of the D4-Enfield lineage. The results of the phylogenetic analysis of Spanish M-F NCRs suggest higher resolution in discriminating strains than did the N-450 analysis. In addition, the results of the analysis of the M-F NCR on the MVs/Madrid.ESP/46.10/ sub-lineage seem to support the potential utility of this region as a tool for epidemiological surveillance complementary to the N-450 region, as previously suggested. Further investigation on this question, as well as the surveillance of new potentially emerging strains with non-standard length M-F NCR are strongly recommended as part of future strategies for measles elimination.
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- 2018
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13. VIGILANCIA MICROBIOLÓGICA DEL SARAMPIÓN Y LA RUBÉOLA EN ESPAÑA. RED DE LABORATORIOS
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Juan Emilio Echevarría, Aurora Fernández García, and Fernando de Ory
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Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
El laboratorio es un elemento imprescindible en la vigilancia del saram-pión y la rubéola, ya que los casos han de ser adecuadamente confirmados para poder estimar la incidencia de forma precisa, las cepas han de ser ca-racterizadas genéticamente para conocer el patrón de circulación de los virus y estudiar de forma completa los brotes y las cadenas de transmisión y la susceptibilidad de la población debe de ser determinada mediante encuestas de seroprevalencia. El diagnóstico de laboratorio de las infecciones agudas por estos agentes se basa en la detección de la respuesta inmune específica de clase IgM, que debe de complementarse con la detección del genoma del virus en exudado faríngeo y/u orina para poder alcanzar un rendimiento diagnóstico óptimo, especialmente si la recogida de las muestras es muy temprana. El genotipado de la cepa se realiza por secuenciación genómica de acuerdo a protocolos de referencia de la OMS. La vigilancia de labo-ratorio de sarampión y rubéola en España se estructura en forma de red, con laboratorios autonómicos de capacidades diferentes y un Laboratorio Nacional de Referencia (LNR), que es el Centro Nacional de Microbiología, que garantiza la disponibilidad de las técnicas en todo el territorio nacional, vela por la calidad de los resultados y representa a la Red Nacional en la Red Europea de laboratorios. El LNR está actualmente implantando nuevas he-rramientas de caracterización molecular basadas en regiones hipervariables del genoma para la caracterización de las cepas a nivel subgenotípico y su aplicación a la vigilancia.
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- 2015
14. The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections
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Fátima Amaro, María P. Sánchez-Seco, Ana Vázquez, Maria J. Alves, Líbia Zé-Zé, Maria T. Luz, Teodora Minguito, Jesús De La Fuente, and Fernando De Ory
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Zika virus ,dengue virus ,secondary infections ,cross-reactions ,IgA ,IgG avidity tests ,Microbiology ,QR1-502 - Abstract
In the absence of viremia, the diagnostics of Zika virus (ZIKV) infections must rely on serological techniques. In order to improve the serological diagnosis of ZIKV, ZIKV-IgA and ZIKV-IgG avidity assays were evaluated. Forty patients returning from ZIKV endemic areas, with confirmed or suspected ZIKV infections were studied. Samples were classified as early acute, acute and late acute according to the number of days post illness onset. Low avidity IgG was only detected at acute and late acute stages and IgA mostly at the early acute and acute stages. The date of sampling provides useful information and can help to choose the best technique to use at a determined moment in time and to interpret low avidity IgG and IgA results, improving the serological diagnosis of ZIKV.
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- 2019
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15. Casos autodeclarados de síndrome gripal en trabajadores sanitarios españoles durante la pandemia de gripe A (H1N1) 2009 Self-reported cases of influenza among Spanish healthcare workers during the 2009 influenza A(H1N1) pandemic
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Julián Olalla, Fernando de Ory, Inmaculada Casas, Javier García-Alegría, and Francisco Rivas-Ruiz
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Trabajadores sanitarios ,Gripe A (H1N1) 2009 ,Vacuna ,Healthcare workers ,Influenza A (H1N1) 2009 ,Vaccination ,Public aspects of medicine ,RA1-1270 - Abstract
Objetivos: Describir la prevalencia de síndrome gripal en el invierno de 2009 y los factores asociados a su ocurrencia. Método: Estudio transversal en 18 hospitales españoles. Los voluntarios respondieron un cuestionario de salud, informando sobre si habían sufrido síndrome gripal y su estado vacunal. Resultados: Participaron 1289 trabajadores sanitarios, y de ellos, 72 (5,6%) refirieron gripe en su familia, 195 (15,1%) se vacunaron frente al virus A/California/7/2009/H1N1 y 75 (5,8%, intervalo de confianza del 95% [IC95%]: 4,5-7,1) sufrieron síndrome gripal. Hubo diferencias entre comunidades autónomas. En el análisis de regresión logística, se asoció a síndrome gripal trabajar en la Comunidad de Madrid (odds ratio [OR]=8,31, IC95%: 1.05-65.39), tener casos de gripe en la familia (OR=2,84, IC95%: 1,41-5,73) y no estar vacunado frente a la gripe A (OR=2,68, IC95%: 1,05-6,82). Conclusiones: La presencia de casos en la familia y la comunidad donde se trabaja determinaron una diferente prevalencia de síndrome gripal. La vacuna se asoció a una menor prevalencia de la enfermedad.Objectives: To describe the prevalence of influenza-like syndrome in winter 2009 and the factors associated with its occurrence. Methods: A cross-sectional study was carried out in 18 hospitals in Spain. Volunteers completed a health questionnaire in which they reported the occurrence of influenza-like syndrome and vaccination and demographic status. Results: A total of 1,289 healthcare workers participated. Of these, 72 (5.6%) reported influenza in their family, 195 (15.1%) had been vaccinated against the A/California/7/2009/H1N1 virus and 75 (5.8%, 95%CI: 4.5-7.1%) had been diagnosed with influenza like-syndrome. There were differences among regions. In logistic regression analysis, the following factors were associated with a higher prevalence of influenza-like syndrome: working in Madrid (OR=8.31, 95%CI: 1.05-65.39), the occurrence of cases of influenza in the family (OR=2.84, 95%CI: 1.41-5.73) and not having been vaccinated against influenza A (H1N1) (OR=2.68, 95% CI: 1.05-6.82). Conclusions: Differences in the prevalence of influenza-like syndrome were due to the occurrence of familiar cases and region. Vaccination against influenza A (H1N1) was associated with a lower prevalence of the disease.
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- 2013
16. Genetic Characterization of Rubella Virus Strains Detected in Spain, 1998-2014.
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Alex O Martínez-Torres, María M Mosquera, Fernando De Ory, Alejandro González-Praetorius, and Juan E Echevarría
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Medicine ,Science - Abstract
The National Plan for the Elimination of Rubella was implemented in Spain in 2008 using the logistics of the National Plan for the Elimination of Measles that have been employed since year 2000. Molecular characterization of rubella virus (RUBV) is important for disease surveillance and for monitoring elimination of the disease throughout the world. We describe the first complete series of data regarding the circulation of RUBV genotypes in Spain. The 739-nucleotide fragment designated by the WHO for RUBV genotyping was sequenced in 88 selected cases collected from 1998 to 2014. Five genotypes were identified: 1E, 2B, 1J, 1I, and 1a. Genotype 1E was predominant between 1998 and 2003 but was replaced by genotype 2B, which was detected in sporadic cases in 2004, 2006, 2008, 2012, 2013 and 2014. There was an outbreak of genotype 2B in Algeciras (Andalusia) in 2008. Genotype 1J caused an outbreak in Madrid in 2004/2005 and sporadic cases in 2005 and 2007. Genotype 1I was found to have infected an immune-suppressed patient with neurological symptoms in 2008. Finally, vaccine strain RA 27/3 was detected in three sporadic cases, two of them immune-suppressed and without a recent history of vaccination. This suggests that during these years there were a series of imported sporadic cases and outbreaks, confirming the findings of epidemiological data analysis. The importation sources were generally consistent with our geographic and cultural ties, mainly with Europe (genotypes 1E, 2B, 1I) and Latin America (1J).
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- 2016
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17. Lymphocytic Choriomeningitis Virus–associated Meningitis, Southern Spain
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Mercedes Pérez-Ruiz, José-María Navarro-Marí, María-Paz Sánchez-Seco, María-Isabel Gegúndez, Gustavo Palacios, Nazir Savji, W. Ian Lipkin, Giovanni Fedele, and Fernando de Ory-Manchón
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Lymphocytic choriomeningitis virus ,meningitis ,Spain ,encephalitis ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Lymphocytic choriomeningitis virus (LCMV) was detected in 2 patients with acute meningitis in southern Spain within a 3-year period. Although the prevalence of LCMV infection was low (2 [1.3%] of 159 meningitis patients), it represents 2.9% of all pathogens detected. LCMV is a noteworthy agent of neurologic illness in immunocompetent persons.
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- 2012
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18. Infección asintomática por el virus influenza H1N1 (2009) en personal sanitario. Estudio Marbegrip. Resultados preliminares
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Julián Olalla Sierra, Fernando de Ory Manchón, Inmaculada Casas Flecha, Natalia Montiel Quezel-Guerraz, Daniel Salas Bravo, and Grupo de Trabajo para el Estudio MARBEGRIP
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Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
Fundamento: La proporción de individuos asintomáticos que se infectan por gripe AH1N1v varía según las series. Los sanitarios están expuestos al virus de la gripe AH1N1v por su condición laboral, por lo que cabe esperar una prevalencia elevada de individuos con serología positiva sin presentar cuadro clínico asociado. El objetivo del estudio fue determinar la prevalencia de sanitarios asintomáticos con serología positiva para el virus de la gripe AH1N1v. Métodos: Se propuso un estudio prospectivo de cohortes en personal hospitalario en función de un hipotético gradiente decreciente de exposición al virus: personal de urgencias, área médica, área quirúrgica y celadores no de urgencias. Se extrajo sangre de los participantes en septiembre- octubre de 2009, noviembre-diciembre de 2009 y en abril-mayo de 2010, junto con la extracción se rellenaba un cuestionario de salud. Se ofreció la participación voluntaria a los miembros de los diferentes servicios. En sangre se procedió a determinar anticuerpos específicos frente al virus de la gripe AH1N1v por medio de inhibición de la hemaglutinación. Participaron 18 hospitales con un total de 1.371 participantes. Resultados: Se dispone de cuestionario de salud y de resultados serológicos de cuatro hospitales. Se observó una proporción variable de sanitarios asintomáticos con serología positiva sin haber sido vacunados (entre el 5,6! y el 83!). Sólo se vacunaron un 19,4! de los sanitarios, con un porcentaje de serologías positivas también variable (entre un 18,8! y 64,7!). El porcentaje de serologías positivas fue significativamente menor entre los celadores y el resto de categorías profesionales. La vacunación fue mayor entre los médicos que en el resto de estamentos profesionales. Conclusiones: Existe un porcentaje variable de individuos con serología positiva sin haber sufrido síntomas, con claras diferencias geográficas. Se observan también diferencias en la efectividad vacunal en términos serológicos.
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- 2011
19. Comparative Evaluation of Indirect Immunofluorescence and NS-1-Based ELISA to Determine Zika Virus-Specific IgM
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Fernando De Ory, María Paz Sánchez-Seco, Ana Vázquez, María Dolores Montero, Elena Sulleiro, Miguel J. Martínez, Lurdes Matas, Francisco J. Merino, and Working Group for the Study of Zika Virus Infections
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Zika virus ,dengue viruses ,flavivirus ,ELISA ,indirect immunofluorescence ,plaque reduction neutralization test ,polymerase chain reaction ,cross-reactions ,Microbiology ,QR1-502 - Abstract
Differential diagnosis of the Zika virus (ZIKV) is hampered by cross-reactivity with other flaviviruses, mainly dengue viruses. The aim of this study was to compare two commercial methods for detecting ZIKV immunoglobulin M (IgM), an indirect immunofluorescence (IIF) and an enzyme immunoassay (ELISA), using the non-structural (NS) 1 protein as an antigen, both from EuroImmun, Germany. In total, 255 serum samples were analyzed, 203 of which showed laboratory markers of ZIKV infections (PCR-positive in serum and/or in urine and/or positive or indeterminate specific IgM). When tested with IIF, 163 samples were IgM-positive, while 13 samples were indeterminate and 78 were negative. When IIF-positive samples were tested using ELISA, we found 61 positive results, 14 indeterminate results, and 88 negative results. Among the indeterminate cases tested with IIF, ELISA analysis found two positive, two indeterminate, and nine negative results. Finally, 74 of the 78 IIF-negative samples proved also to be negative using ELISA. For the calculations, all indeterminate results were considered to be positive. The agreement, sensitivity, and specificity between ELISA and IIF were 60.2%, 44.9%, and 94.9%, respectively. Overall, 101 samples showed discrepant results; these samples were finally classified on the basis of other ZIKV diagnostic approaches (PCR-positive in serum and/or in urine, IgG determinations using IIF or ELISA, and ZIKV Plaque Reduction Neutralization test—positive), when available. A final classification of 228 samples was possible; 126 of them were positive and 102 were negative. The corresponding values of agreement, sensitivity, and specificity of IIF were 86.0%, 96.8%, and 72.5%, respectively. The corresponding figures for ELISA were 81.1%, 65.9%, and 100%, respectively. The ELISA and IIF methods are both adequate approaches for detecting ZIKV-specific IgM. However, considering their respective weaknesses (low sensitivity in ELISA and low specificity in IIF), serological results must be considered jointly with other laboratory results.
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- 2018
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20. ENCUESTAS SEROEPIDEMIOLÓGICAS EN ENFERMEDADES NO INMUNOPREVENIBLES Y SU INTERÉS EN SALUD PÚBLICA
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Fernando de Ory Manchón
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Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
La seroepidemiología es el estudio epidemiológico que se realiza mediante del uso de pruebas serológicas para detectar infección; es de aplicación a las infecciones en general, y a las enfermedades inmunoprevenibles. Entre otras aplicaciones, los estudios seroepidemiológicos son de utilidad para determinar las poblaciones en riesgo para una enfermedad; para evaluar los mecanismos de transmisión, y para determinar los grupos de población que son críticos para mantener la transmisión de los agentes infecciosos. Se han analizado resultados derivados de estudios de seroprevalencia realizados en España en relación con enfermedades no inmunoprevenibles de transmisión vertical (Toxoplasma gondii, Treponema pallidum, citomegalovirus, virus herpes simple tipo 2, y parvovirus humano B19), enfermedades de transmisión sanguínea (virus de la hepatitis C, virus de la inmunodeficiencia humana y Trypanosoma cruzi) y enfermedades emergentes (Virus del Nilo occidental, virus Toscana, y virus de la coriomeningitis linfocitaria). Se observa reducción en la seroprevalencia en la población autóctona española frente a Toxoplasma gondii, Treponema pallidum, citomegalovirus y virus de la inmunodeficiencia humana. Los patrones obtenidos en población inmigrante reflejan la situación de estas enfermedades en sus países de origen; pudiendo ser causa de situaciones de impacto en la salud pública (Trypanosoma cruzi, en relación con la donación de sangre). Se pone en evidencia la circulación en España de virus del Nilo Occidental, y la importancia de la infección por virus Toscana.
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- 2009
21. Towards elimination: measles susceptibility in Australia and 17 European countries
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Nick Andrews, Annedore Tischer, Annette Siedler, Richard G Pebody, Christopher Barbara, Suzanne Cotter, Arnis Duks, Nina Gacheva, Kriz Bohumir, Kari Johansen, Joel Mossong, Fernando de Ory, Katarina Prosenc, Margareta Sláciková, Heidi Theeten, Marios Zarvou, Adriana Pistol, Kalman Bartha, Dani Cohen, Jo Backhouse, and Algirdas Griskevicius
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Public aspects of medicine ,RA1-1270 - Abstract
OBJECTIVE: To evaluate age-specific measles susceptibility in Australia and 17 European countries. METHODS: As part of the European Sero-Epidemiology Network 2 (ESEN2), 18 countries collected large national serum banks between 1996 and 2004. These banks were tested for measles IgG and the results converted to a common unitage to enable valid intercountry comparisons. Historical vaccination and disease incidence data were also collected. Age-stratified population susceptibility levels were compared to WHO European Region targets for measles elimination of < 15% in those aged 2-4 years, < 10% in 5-9-year-olds and < 5% in older age groups. FINDINGS: Seven countries (Czech Republic, Hungary, Luxembourg, Spain, Slovakia, Slovenia and Sweden) met or came very close to the elimination targets. Four countries (Australia, Israel, Lithuania and Malta) had susceptibility levels above WHO targets in some older age groups indicating possible gaps in protection. Seven countries (Belgium, Bulgaria, Cyprus, England and Wales, Ireland, Latvia and Romania) were deemed to be at risk of epidemics as a result of high susceptibility in children and also, in some cases, adults. CONCLUSION: Although all countries now implement a two-dose measles vaccination schedule, if the WHO European Region target of measles elimination by 2010 is to be achieved higher routine coverage as well as vaccination campaigns in some older age cohorts are needed in some countries. Without these improvements, continued measles transmission and outbreaks are expected in Europe.
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- 2008
22. SEROEPIDEMIOLOGÍA FRENTE A CITOMEGALOVIRUS EN LA COMUNIDAD DE MADRID
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Fernando de Ory Manchón, Juan Carlos Sanz Moreno, Rosario Castañeda López, Rosa Ramírez Fernández, Pilar León Rega, and Isabel Pachón del Amo
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Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
Fundamento: La infección por citomegalovirus (CMV) es frecuentemente asintomática. Sin embargo, constituye una importante causa de infección congénita y de patología severa en sujetos inmunodeprimidos, por lo que representa un problema en Salud Pública. El objeto de este trabajo fue conocer la prevalencia de IgG frente a CMV (IgG-CMV) en la población general madrileña. Métodos: Estudio transversal en el que se detectó IgG-CMV en una muestra representativa de la población general de la Comunidad de Madrid entre 2 y 60 años (n=2030). Se realizó un muestro bietápico estratificado por conglomerados (octubre de 1993 y febrero de 1994). Para el análisis estadístico se emplearon los test de 2 y 2 de tendencia lineal y se calcularon los porcentajes de seroprevalencia y los odds ratios con intervalos de confianza del 95%. Resultados: La seroprevalencia global fue del 62,8% (IC95%: 60,6-64,9); 66,7% (IC95%: 63,7-69,5) en mujeres y del 58,4% (IC95%: 55,2-61,5) en hombres. Se observó una asociación significativa entre el aumento de la edad y el incremento de la seroprevalencia. Fueron factores significativos de riesgo la consulta al dentista, antecedentes de cirugía, acupuntura y tatuajes. Los estudios superiores constituyeron un factor protector. Conclusión: Aunque los factores de riesgo detectados indican una posible transmisión vía sanguínea, la elevada prevalencia apunta a la existencia de otras vías mas comunes. El aumento de la seroprevalencia dependiente de la edad sugiere que se produce un número importante de infecciones en la edad adulta. No se puede excluir, sin embargo, que este aumento responda a un efecto cohorte debido a mejoras socioeconómicas similar al detectado para otros virus.
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- 2001
23. Importation of West Nile Virus Infection from Nicaragua to Spain
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Begoña Monge Maillo, Rogelio López-Vélez, Francesca F. Norman, Fernando de Ory, María Paz Sanchez-Seco, and Cesare Giovanni Fedele
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Nicaragua ,Spain ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2008
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24. First report of sylvatic DENV-2-associated dengue hemorrhagic fever in West Africa.
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Leticia Franco, Gustavo Palacios, José Antonio Martinez, Ana Vázquez, Nazir Savji, Fernando De Ory, María Paz Sanchez-Seco, Dolores Martín, W Ian Lipkin, and Antonio Tenorio
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
Dengue virus (DENV) circulates in human and sylvatic cycles. Sylvatic strains are both ecologically and evolutionarily distinct from endemic viruses. Although sylvatic dengue cycles occur in West African countries and Malaysia, only a few cases of mild human disease caused by sylvatic strains and one single case of dengue hemorrhagic fever in Malaysia have been reported. Here we report a case of dengue hemorrhagic fever (DHF) with thrombocytopenia (13000/µl), a raised hematocrit (32% above baseline) and mucosal bleeding in a 27-year-old male returning to Spain in November 2009 after visiting his home country Guinea Bissau. Sylvatic DENV-2 West African lineage was isolated from blood and sera. This is the first case of DHF associated with sylvatic DENV-2 in Africa and the second case worldwide of DHF caused by a sylvatic strain.
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- 2011
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25. Human West Nile Virus Infection, Catalonia, Spain
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Domingo Bofill, Cristina Domingo, Neus Cardeñosa, Joan Zaragoza, Fernando de Ory, Sofia Minguell, María Paz Sánchez-Seco, Angela Domínguez, and Antonio Tenorio
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West Nile virus ,serosurvey ,flavivirus ,Spain ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2006
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26. Brote de sarampión en el área sanitaria de Guadalajara (España): dificultad en el diagnóstico microbiológico en la era de su eliminación
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Sergio Gilaberte-Reyzabal, Begoña Caballero-López, Mayte Pérez-Olmeda, Alejandro González-Praetorius, Aurora Fernández-García, Elena Román-Marcos, M. Victoria García-Rivera, Juan Emilio Echevarría-Mayo, and Fernando de Ory-Machón
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Microbiology (medical) ,business.industry ,Medicine ,business ,Humanities - Abstract
Resumen Introduccion En Espana, al igual que en otros paises donde el sarampion endemico ha sido eliminado, es necesario utilizar de forma rutinaria las herramientas diagnosticas que confirmen los casos para su prevencion y control de la diseminacion. Se describen los diferentes ensayos microbiologicos utilizados para su diagnostico durante un brote de sarampion en 2019 en la provincia de Guadalajara (Espana). Metodos Las pruebas serologicas y moleculares se realizaron en el laboratorio de Microbiologia del Hospital Universitario de Guadalajara y en el Centro Nacional de Microbiologia del Instituto de Salud Carlos III (Majadahonda, Espana). Los datos de los pacientes se obtuvieron del sistema epidemiologico de vigilancia. Resultados Se diagnosticaron de sarampion un total de 43 pacientes por metodos microbiologicos: 29 casos por PCR (exudado faringeo u orina) junto con IgM especifica positiva, 11 pacientes solamente por PCR, y 3 pacientes exclusivamente por presencia de IgM. El genotipo D8 fue identificado en 35 pacientes y el genotipo A en 2 casos descartados como postvacunal. La PCR en suero fue positiva en 11 de 14 pacientes con ausencia de IgM en su primera muestra recogida de suero. Once casos confirmados habian recibido una o 2 dosis de la vacuna. Doce adultos fueron ingresados, todos diagnosticados de hepatitis. Conclusiones La combinacion de pruebas moleculares y la presencia de IgG e IgM especificas son necesarias para un diagnostico correcto y la clasificacion de los pacientes como fallo vacunal (primario o secundario). El genotipado es una herramienta fundamental para la correcta clasificacion de los pacientes en el contexto de un programa de eliminacion del sarampion.
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- 2022
27. Comparative evaluation of assays for IgM detection of rubella and measles infections
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Desirée Dafouz, Fernando de Ory, José Luis Muñoz, Pilar Balfagón, Mayte Pérez Olmeda, A. García, Juan Carlos Sanz, Jesús de la Fuente, María Ángeles Murillo, and Juan Camacho
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0301 basic medicine ,Microbiology (medical) ,030106 microbiology ,Antibodies, Viral ,medicine.disease_cause ,Measles ,Rubella ,Comparative evaluation ,Serology ,law.invention ,Measles virus ,03 medical and health sciences ,0302 clinical medicine ,law ,medicine ,Humans ,Electrochemiluminescence ,030212 general & internal medicine ,Chemiluminescence ,biology ,business.industry ,Rubella virus ,biology.organism_classification ,medicine.disease ,Virology ,Immunoglobulin M ,business - Abstract
Background Serological diagnosis of infections due to measles and rubella viruses is done by IgM detection. The aim of this study was to comparatively evaluate commercial systems for detecting IgM against both viruses, including those of ELISA, in indirect and capture formats, chemiluminescence and electrochemiluminescence. Methods Seven (for rubella) and six (for measles) assays were studied. One hundred and sixty two samples were included in the study (from 90 rubella and 72 measles cases), and all were analyzed in all the assays. Results The ranges of sensitivity, specificity and agreement for rubella were 94.8–100%, 52.4–100% and 75.5–98.1%, respectively. The corresponding ranges for measles assays were 87.0–100%, 53.3–100%, and 73.0–99.4%. Conclusion The best-performing assays were chemiluminescence (for measles and rubella IgM), and electrochemiluminescence (for rubella IgM).
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- 2022
28. Fatal Case of Crimean-Congo Hemorrhagic Fever Caused by Reassortant Virus, Spain, 2018
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Fernando de Ory, L. Hernández, Paz Sánchez-Seco, Almudena Gutiérrez-Arroyo, Carmen Grande, Francisco Díez-Fuertes, Marco Antonio Budiño, Ángeles Garcinuño, César de la Hoz González, Ana Vázquez, Anabel Negredo, Rafael Sánchez-Arroyo, Instituto de Salud Carlos III, Red de Investigación Cooperativa en Enfermedades Tropicales (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), and European Regional Development Fund (ERDF/FEDER)
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Crimean–Congo hemorrhagic fever ,Epidemiology ,viruses ,vector-borne infections ,lcsh:Medicine ,molecular epidemiology ,0302 clinical medicine ,Ticks ,Zoonoses ,phylogenetic analyses ,030212 general & internal medicine ,Phylogenetic analyses ,biology ,Dispatch ,Crimean Congo hemorrhagic fever virus ,Tick-borne illness ,Infectious Diseases ,reassortant ,Molecular epidemiology ,Viruses ,Fatal Case of Crimean-Congo Hemorrhagic Fever Caused by Reassortant Virus, Spain, 2018 ,Hemorrhagic Fever Virus, Crimean-Congo ,Reassortant Viruses ,Microbiology (medical) ,tick-borne illness ,Reassortant virus ,030231 tropical medicine ,RT-PCR ,Genome, Viral ,ticks ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Hyalomma ,medicine ,Humans ,lcsh:RC109-216 ,Reassortant ,business.industry ,lcsh:R ,Vector-borne infections ,medicine.disease ,biology.organism_classification ,Virology ,zoonoses ,Spain ,Hemorrhagic Fever, Crimean ,business - Abstract
In August 2018, a fatal autochthonous case of Crimean-Congo hemorrhagic fever was confirmed in western Spain. The complete sequence of the viral genome revealed circulation of a new virus because the genotype differs from that of the virus responsible for another case in 2016. Practitioners should be alert to possible new cases. This study was partially funded by ISCIII, RD16CIII/0003/0003, “Red de Enfermedades Tropicales,” Subprogram RETICS Plan Estatal de I+D+I 2013-2016, and co-funded by FEDER “Una manera de hacer Europa.” Sí
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- 2021
29. Hantavirus Pulmonary Syndrome in Traveler Returning from Nepal to Spain
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María Luisa Aznar, Miguel J. Martínez, Fernando Salvador, M. Paz Sanchez-Seco, Mateu Espasa, Fernando de Ory, Elena Sulleiro, Adrián Sánchez-Montalvá, Núria Serre-Delcor, Tomás Pumarola, Israel Molina, Candido Diaz-Lagares, Inés Oliveira, and Daniel Molina
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Adult ,Male ,Microbiology (medical) ,Epidemiology ,viruses ,030231 tropical medicine ,India ,lcsh:Medicine ,Hantavirus Pulmonary Syndrome ,Puumala virus ,hantavirus ,lcsh:Infectious and parasitic diseases ,Serology ,Hantavirus Pulmonary Syndrome in Traveler Returning from Nepal to Spain ,respiratory infections ,03 medical and health sciences ,0302 clinical medicine ,Nepal ,Cardiopulmonary syndrome ,Zoonoses ,Research Letter ,Humans ,Medicine ,lcsh:RC109-216 ,030212 general & internal medicine ,Sri Lanka ,Hantavirus ,Travel ,Hantavirus pulmonary syndrome ,traveler ,business.industry ,lcsh:R ,cardiopulmonary syndrome ,Respiratory infections ,virus diseases ,Virology ,zoonoses ,American sigmodontine hantaviruses ,Infectious Diseases ,Spain ,Viruses ,European arvicoline hantaviruses ,Sri lanka ,Hantavirus Infection ,business ,Traveler - Abstract
Most human hantavirus infections occur in Asia, but some cases have been described in Europe in travelers returning from Asia. We describe a case of hantavirus pulmonary syndrome in a previously healthy traveler occurring shortly after he returned to Spain from Nepal. Serologic tests suggested a Puumala virus-like infection. Sí
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- 2020
30. Pertussis seroprevalence among adults of reproductive age (20–39 years) in fourteen European countries
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Waldemar Rastawicki, H. O. Hallander, Kris Huygen, Julie Haider, Arno Hänninen, Evi Petridou, Sabrina Bacci, Clara M. Ausiello, Margaretha Ljungman, Fernando de Ory Manchón, Tine Dalby, Vilnele Lipnickiene, Lena Wehlin, Sharon Kühlmann-Berenzon, Tove Karin Herstad, Audun Aase, Zsuzsanna Molnár, Carla Rio, Alex-Mikael Barkoff, Qiushui He, Ilias Galanis, Odette Popovici, Denis Pierard, Maria Carollo, Supporting clinical sciences, Microbiology and Infection Control, and Clinical Biology
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Adult ,Male ,Microbiology (medical) ,Veterinary medicine ,Bordetella pertussis ,Vaccination Coverage ,Whooping Cough ,Reproductive age ,Pertussis toxin ,Europe/epidemiology ,Whooping Cough/epidemiology ,Pathology and Forensic Medicine ,Young Adult ,Elisa kit ,Age groups ,Seroepidemiologic Studies ,Humans ,Immunology and Allergy ,Medicine ,Seroprevalence ,Medicine(all) ,biology ,business.industry ,Incidence ,Incidence (epidemiology) ,Antibodies, Bacterial/blood ,General Medicine ,biology.organism_classification ,Antibodies, Bacterial ,Vaccination Coverage/statistics & numerical data ,Europe ,Vaccination ,Immunoglobulin G/blood ,Immunoglobulin G ,Female ,business ,Bordetella pertussis/immunology - Abstract
The reported incidence of pertussis in European countries varies considerably. We aimed to study specific Bordetella pertussis seroprevalence in Europe by measuring serum IgG antibody levels to pertussis toxin (anti-PT IgG). Fourteen national laboratories participated in this study including Belgium, Denmark, Finland, Greece, Hungary, Italy, Lithuania, Malta, Norway, Poland, Portugal, Romania, Spain, and Sweden. Each country collected approximately 250 samples (N = 7903) from the age groups 20-29 years (N = 3976) and 30-39 years (N = 3927) during 2010-2013. Samples were anonymous residual sera from diagnostic laboratories and were analyzed at the national laboratories by a Swedish reference method, a commercial ELISA kit, or were sent to Sweden for analysis. The median anti-PT IgG concentrations ranged from 4 to 13.6 IU/mL. The proportion of samples with anti-PT IgG ≥100 IU/mL, indicating a recent infection ranged from 0.2% (Hungary) to 5.7% (Portugal). The highest proportion of sera with anti-PT IgG levels between 50 and
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- 2021
31. Measles outbreak in the sanitary area of Guadalajara (Spain): difficulty in microbiological diagnosis in the era of its elimination
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Alejandro González-Praetorius, Aurora Fernández-García, Mayte Pérez-Olmeda, M. Victoria García-Rivera, Begoña Caballero-López, Sergio Gilaberte-Reyzabal, Elena Román-Marcos, Fernando de Ory-Machón, and Juan Emilio Echevarría-Mayo
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Adult ,Immunoglobulin M ,Measles virus ,Spain ,Humans ,Antibodies, Viral ,Measles ,Disease Outbreaks - Abstract
In Spain, like in other countries where endemic measles has been eliminated, there is a need for available diagnostic tools for confirming any cases in order to prevent and control its transmission. We describe the different microbiological tests used for the diagnosis of measles during an outbreak that occurred in 2019 in the province of Guadalajara (Spain).Serological and molecular tests were performed at the Microbiology laboratory of the Guadalajara University Hospital and at the National Center for Microbiology of the Carlos III Health Institute (Majadahonda, Spain). Patient data were obtained from the surveillance system.A total of 43 patients had a laboratory diagnosis of measles: 29 cases by PCR (pharyngeal exudate or urine) and positive specific IgM, 11 cases by PCR, and 3 cases only by a positive IgM. Genotype D8 was identified in 35 confirmed cases and genotype A in 2 that were discarded as post-vaccination cases. PCR was positive in the acute sera of 11 out of 14 patients with a negative IgM. Eleven confirmed cases had recieved one or 2 vaccine doses. Twelve adult patients were hospitalizated, all of them with a diagnostic of hepatitis.The combination of molecular tests and the presence of specific IgG and IgM are necessary for a correct diagnosis of measles and also to classify patients with a breakthrough infection or vaccine failures (primary or secondary). Genotyping is essential for the correct classification of the patients in the context of a measles elimination program.
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- 2021
32. Caracterización de los resultados correspondientes al brote de sarampión de Madrid de 2019 en las muestras clínicas procesadas en el Laboratorio Regional de Salud Pública
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Teresa García Gómez, Fernando de Ory, Juan Carlos Sanz Moreno, Juan Emilio Echevarría, Isabel Vadillo, and Aurora Arjones Fernández
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0301 basic medicine ,igm ,business.industry ,rt-pcr ,lcsh:Public aspects of medicine ,030106 microbiology ,Outbreak ,Rubella virus ,muestras clínicas ,lcsh:RA1-1270 ,medicine.disease ,medicine.disease_cause ,Virology ,Rubella ,Measles ,Serology ,Medical Terminology ,03 medical and health sciences ,PHARYNGEAL EXUDATE ,0302 clinical medicine ,medicine ,030212 general & internal medicine ,business ,Medical Assisting and Transcription ,sarampión - Abstract
En la primavera de 2019 se ha producido en la Comunidad de Madrid un aumento de casos de sarampión. El objetivo de este estudio es describir el tipo de muestras clínicas estudiadas y los resultados obtenidos en este brote. Entre el 1 de abril y el 25 de junio de 2019 se procesaron 193 muestras biológicas correspondientes a 109 sospechas clínicas de sarampión. Las determinaciones serológicas (IgM e IgG frente a sarampión y rubéola) se llevaron a cabo por ELISA indirecto. La amplificación de ácidos nucleicos de los virus del sarampión y la rubéola se efectuó mediante RT-PCR. Se confirmaron 44 casos de sarampión. Dos de ellos habían sido vacunados muy recientemente y ambos presentaban el genotipo vacunal A. Los amplificados de todas las cepas salvajes (21) eran D8. En esta serie, el tiempo medio observado entre recepción de muestras y obtención del resultado positivo fue de 0,5 días. En 32 casos confirmados se dispuso de muestras tanto de exudado faríngeo para RT-PCR como de sangre para serología y en 32 la RT-PCR resultó positiva (sensibilidad 100%, IC95% 86,7- 99,7) mientras que en 22 la IgM fue positiva (sensibilidad 68,8%, IC95% 49,9- 83,3). Inversamente, en 49 sospechas descartadas se dispuso de muestras de exudado faríngeo para RT-PCR como de sangre para serología y en 49 la RT-PCR resultó negativa (especificidad 100%, IC95% 90,9- 99,8). En 1 muestra de una sospecha descartada la IgM fue positiva (especificidad 98,0%, IC95% 87,8- 99,9). En la etapa de eliminación del sarampión y la rubéola los estándares de vigilancia deben ir dirigidos a detectar todos los posibles casos. Para ello es preciso la obtención en tiempos correctos de las muestras biológicas adecuadas (suero y especialmente exudado faríngeo).
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- 2019
33. New circulation of genotype V of Crimean-Congo haemorrhagic fever virus in humans from Spain
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Moncef Belhassen-García, Lía Monsalve Arteaga, Juan Luis Muñoz Bellido, Jorge García Criado, María Belén Vicente Santiago, Nuria Leralta, María Carmen Vieira Lista, Jesús Ángel Sánchez Serrano, Antonio Muro, Amparo López Bernús, Fernando de Ory Manchón, Montserrat Alonso Sardón, María Paz Sánchez Seco, Ana Isabel Negredo, Instituto de Salud Carlos III - ISCIII, European Regional Development Fund (ERDF/FEDER), Instituto de Salud Carlos III, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), and Red de Investigación Cooperativa en Enfermedades Tropicales
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Male ,Crimean–Congo hemorrhagic fever ,Critical Care and Emergency Medicine ,Pulmonology ,Epidemiology ,RC955-962 ,Social Sciences ,Geographical locations ,0302 clinical medicine ,Arctic medicine. Tropical medicine ,Genotype ,Psychology ,Prospective Studies ,030212 general & internal medicine ,Phylogeny ,Neglected tropical diseases ,media_common ,Aged, 80 and over ,Geography ,Animal Behavior ,Middle Aged ,Europe ,Phylogeography ,Infectious Diseases ,Biogeography ,Hemorrhagic Fever Virus, Crimean-Congo ,Crimean-Congo hemorrhagic fever ,Ethnology ,Female ,Public aspects of medicine ,RA1-1270 ,Research Article ,Medical conditions ,030231 tropical medicine ,Crimean-Congo haemorrhagic fever virus ,Viral diseases ,Respiratory Disorders ,03 medical and health sciences ,Genetics ,medicine ,Humans ,media_common.cataloged_instance ,European Union ,European union ,Aged ,Medicine and health sciences ,Evolutionary Biology ,Behavior ,Population Biology ,Tropical diseases ,Ecology and Environmental Sciences ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,Sequence Analysis, DNA ,medicine.disease ,Cross-Sectional Studies ,Immunoglobulin M ,Spain ,Immunoglobulin G ,Medical Risk Factors ,DNA, Viral ,Respiratory Infections ,Earth Sciences ,Hemorrhagic Fever, Crimean ,Animal Migration ,People and places ,Zoology ,Viral hemorrhagic fevers ,Population Genetics - Abstract
Background Crimean-Congo haemorrhagic fever (CCHF) is a widespread tick-borne viral disease caused by the Crimean-Congo haemorrhagic fever virus (CCHFV). CCHFV has been implicated in severe viral haemorrhagic fever outbreaks. During the summer of 2016, the first two cases with genotype III (Africa 3) were reported in Spain. The first objective of our study was to determine the presence of CCHFV among patients with febrile illness during the spring and summer periods in 2017 and 2018. Finally, we perform a phylogenetic analysis to determine the genotype of the virus. Methodology We prospectively evaluated patients aged 18 years and older who came to the emergency department at the Salamanca’s University Hospital (HUS) with fever. Specific IgM and IgG antibodies against CCHFV by ELISA and one immunofluorescence assay against two different proteins (nucleoprotein and glycoprotein C) was done. Moreover, molecular detection by Real Time PCR was performed in all collected samples. A phylogenetic analysis was carried out to genetically characterize CCHFV detected in this study. Principal findings A total of 133 patients were selected. The mean age was 67.63 years and 60.9% were male. One-third of the patients presented an acute undifferentiated febrile illness. Three patients had anti-CCHFV IgG antibodies, suggesting a previous infection. One patient had anti-CCHFV IgM antibodies and a confirmatory RT-PCR. Phylogenetic analysis indicated that the virus corresponds to the European genotype V. This patient came to the emergency department at HUS in August 2018 presenting an acute febrile syndrome with thrombopenia and liver impairment. Conclusions We describe a new circulation of European genotype V CCHFV in Spain. Moreover, this study suggests that CCHFV is an identifiable cause of febrile illness of unknown origin in Spain. Thus, CCHF could be suspected in patients with fever, liver damage, and/or haemorrhagic disorders, particularly in people with risk activities who present in the spring or summer., Author summary Crimean Congo hemorrhagic fever (CCHF) is a widespread tick-borne viral disease caused by a Nairovirus of the Bunyaviridae family. CCHFV has been considered to be one of the eight priority emergent pathogens for the last 3 years by the World Health Organization (WHO), requiring urgent attention in Research, Development and Innovation (R&D&I) because of its epidemic potential in the near future In this study, we describe a new circulation of European genotype V CCHFV in Spain. Moreover, this study suggests that CCHFV is an identifiable cause of febrile illness of unknown origin in Spain. Thus, CCHF could be suspected in patients with fever, liver damage, and/or haemorrhagic disorders, particularly in people with risk activities who present in the spring or summer.
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- 2021
34. Identification of Immunological Parameters as Predictive Biomarkers of Relapse in Patients with Chronic Myeloid Leukemia on Treatment-Free Remission
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José Alcamí, María Rosa López-Huertas, Miguel Piris-Villaespesa, Sara Rodríguez-Mora, Alejandro Luna, Guiomar Bautista, Vicente Planelles, Lorena Vigón, Mayte Pérez-Olmeda, Mayte Coiras, Daniel Fuertes, Valentín García-Gutiérrez, Miguel Galán, Elena Mateos, Esther San José, Juan Luis Steegmann, José Rivera-Torres, Fernando de Ory, Fundación para la Investigación Biomédica del Hospital Universitario Ramón y Caja, Ministerio de Economía y Competitividad (España), Red Española de Investigación en SIDA, Instituto de Salud Carlos III, European Regional Development Fund (ERDF/FEDER), and Instituto de Salud Carlos III - ISCIII
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Proteases ,Biotecnología ,lcsh:Medicine ,Article ,Proinflammatory cytokine ,03 medical and health sciences ,Tratamiento médico ,0302 clinical medicine ,chronic myeloid leukemia ,hemic and lymphatic diseases ,Cytotoxic T cell ,Medicine ,030304 developmental biology ,0303 health sciences ,treatment-free remission ,immunological response ,business.industry ,lcsh:R ,Myeloid leukemia ,Leucemia mielógena crónica BCR-ABL positiva ,General Medicine ,Cáncer ,Natural killer T cell ,Discontinuation ,030220 oncology & carcinogenesis ,Cancer research ,business ,Tyrosine kinase ,CD8 - Abstract
BCR-ABL is an aberrant tyrosine kinase responsible for chronic myeloid leukemia (CML). Tyrosine kinase inhibitors (TKIs) induce a potent antileukemic response mostly based on the inhibition of BCR-ABL, but they also increase the activity of Natural Killer (NK) and CD8+ T cells. After several years, patients may interrupt treatment due to sustained, deep molecular response. By unknown reasons, half of the patients relapse during treatment interruption, whereas others maintain a potent control of the residual leukemic cells for several years. In this study, several immunological parameters related to sustained antileukemic control were analyzed. According to our results, the features more related to poor antileukemic control were as follows: low levels of cytotoxic cells such as NK, (Natural Killer T) NKT and CD8±, TCR&gamma, &beta, + T cells, low expression of activating receptors on the surface of NK and NKT cells, impaired synthesis of proinflammatory cytokines or proteases from NK cells, and HLA-E*0103 homozygosis and KIR haplotype BX. A Random Forest algorithm predicted 90% of the accuracy for the classification of CML patients in groups of relapse or non-relapse according to these parameters. Consequently, these features may be useful as biomarkers predictive of CML relapse in patients that are candidates to initiate treatment discontinuation.
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- 2020
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35. Enfermedades asociadas a flebovirus trasmitidos por flebótomos: ¿qué riesgo tenemos en España?
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Berta Suarez, Susana Monge, Fernando de Ory, Ricardo Molina, Lucía García San Miguel, Javier Lucientes, Jordi Figuerola, Fernando Simón, Beatriz Fernández-Martínez, Ana Vázquez, M. Paz Sanchez-Seco, and M. Jose Sierra
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0301 basic medicine ,Microbiology (medical) ,Phlebovirus ,030106 microbiology ,Flebótomos ,España ,Evaluación de riesgo ,03 medical and health sciences ,0302 clinical medicine ,Spain ,Phlebotomus ,Vector-borne disease ,Flebovirus ,030212 general & internal medicine ,Enfermedades vectoriales ,Risk assessment - Abstract
[ES] Los virus del género Phlebovirus, transmitidos por dípteros de la familia Psychodidae, son una causa de síndrome febril autolimitado durante el verano-otoño en los países mediterráneos. En el caso del virus Toscana, pueden ser causa de meningitis y meningoencefalitis. En España se ha detectado la presencia de los virus Toscana, Granada, Nápoles, Sicilia, Arbia y Arrabida-like. La presencia casi generalizada de vectores del género Phlebotomus, especialmente de Phlebotomus perniciosus, en los que se han detectado varios de estos virus, hace muy probable que aparezcan de manera regular infecciones en humanos en nuestro país, siendo este riesgo moderado para el virus Toscana y bajo para el resto, en las zonas con mayor actividad del vector. La mayor parte de las enfermedades pasarían inadvertidas y solo el virus Toscana puede tener un mayor impacto por la aparición de casos graves., [EN] The genera Phlebovirus transmitted by Diptera belonging to the Psychodidae family are a cause of self-limited febrile syndrome in the Mediterranean basin in summer and autumn. Toscana virus can also cause meningitis and meningoencephalitis. In Spain, Toscana, Granada, Naples, Sicily, Arbia and Arrabida-like viruses have been detected. The almost widespread distribution of Phlebotomus genus vectors, and especially Phlebotomus perniciosus, in which several of these viruses have been detected, makes it very likely that there will be regular human infections in our country, with this risk considered moderate for Toscana virus and low for the other ones, in areas with the highest vector activity. Most of the infections would be undiagnosed, while only Toscana virus would have a greater impact due to the potential severity of the illness.
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- 2020
36. Zika virus infection in pregnant travellers and impact on childhood neurodevelopment in the first two years of life: A prospective observational study
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Raquel González, Anna Goncé, María-Jesús Pinazo, Victoria Fumadó, Aina Casellas, Paola Castillo, Miguel J. Martínez, Azucena Bardají, Adela Saco, Ana Vázquez, Jaume Ordi, Fernando de Ory, Ana Requena-Méndez, Claudia Fortuny, Laura García-Otero, Carolina Esteve, Elisenda Eixarch, Natalia Rodriguez-Valero, Daniel Camprubí, Laura Salazar, Inés Oliveira, Jessica Navero-Castillejos, Joaquim Gascon, Jose Muñoz, Dolors Salvia, Clara Menéndez, Marina Fuente-Moreno, Elena Marbán-Castro, Marta López, Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades (España), Red de Investigación Cooperativa en Enfermedades Tropicales (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Government of Catalonia (España), Fundación La Caixa, European Regional Development Fund (ERDF/FEDER), and Generalitat de Catalunya
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Pediatrics ,medicine.medical_specialty ,Language function ,030231 tropical medicine ,Neurodevelopment ,Zika virus ,Miscarriage ,Fetal ,03 medical and health sciences ,0302 clinical medicine ,Fetus ,Pregnancy ,Epidemiology ,ZikV Infection ,medicine ,Humans ,030212 general & internal medicine ,Prospective Studies ,Pregnancy Complications, Infectious ,Child ,Children ,Surveillance ,biology ,business.industry ,Zika Virus Infection ,Public Health, Environmental and Occupational Health ,Infant ,Zika Virus ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Brain cysts ,Observational study ,Female ,business - Abstract
The emergence of Zika virus (ZIKV) represents a threat with consequences on maternal and children's health. We aimed to assess the clinical and epidemiological characteristics of pregnant women returning from ZIKV affected areas, and the effects of maternal ZIKV infection on birth outcomes and children's health. This was a hospital-based prospective observational study conducted at the Hospital Clínic of Barcelona and Hospital Sant Joan de Déu, Barcelona, Spain, from January 2016 to February 2020. One hundred and ninety-five pregnant women who had travelled to ZIKV affected areas during pregnancy were recruited. Four women (2.1%) had a confirmed ZIKV infection, 40 women (20.5%) a probable infection, and 151 (77.4%) were negative for ZIKV. Among the ZIKV confirmed cases, a pregnant woman suffered a miscarriage, highly plausible to be associated with ZIKV infection. Brain cysts and microcalcifications were detected in 7% of fetuses or infants from women with confirmed or probable ZIKV infection. Neurodevelopmental delay in the language function was found in 33.3% out of the 21 children evaluated. These findings contribute to the understanding of ZIKV prevalence estimates, and the impact of maternal ZIKV infection on pregnancy outcomes and children's health. Results highlight the importance of long-term surveillance in pregnant travellers and their children. This work was supported by the Government of Spain [grant number PI16/0123, ISCIII-AES Proyectos de Investigación en Salud, 2016]; a predoctoral fellowship from “la Caixa” Foundation (ID 100010434) [fellowship LCF/BQ/ES17/11600006]; the Ministry of Science, Innovation and Universities, Government of Spain through a Ramon y Cajal Grant [RYC-2013-14,512]; RICET, a Tropical Disease Cooperative Research Network in Spain [RD12/0018/0010] cofounded by ISCIII and the Fondo Europeo de Desarrollo Regional (FEDER); the Departament d’Universitats I Recerca de la Generalitat de Catalunya, Spain, AGAUR [grant 2017SGR924]; and ISGlobal is a member of the CERCA Programme, Generalitat de Catalunya. Sí
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- 2020
37. Phlebovirus-associated diseases transmitted by phlebotominae in Spain: Are we at risk?
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Lucía García San Miguel, Jordi Figuerola, Ricardo Molina, Berta Suarez, Susana Monge, Javier Lucientes, Ana Vázquez, M. Jose Sierra, Fernando de Ory, Beatriz Fernández-Martínez, Fernando Simón, and M. Paz Sanchez-Seco
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Phlebovirus ,biology ,Phlebotominae ,Toscana virus ,Meningoencephalitis ,Sandfly fever Naples virus ,biology.organism_classification ,medicine.disease ,Virology ,Mediterranean Basin ,Insect Vectors ,Spain ,Vector (epidemiology) ,parasitic diseases ,medicine ,Animals ,Humans ,Phlebotomus ,Psychodidae - Abstract
The genera Phlebovirus transmitted by Diptera belonging to the Psychodidae family are a cause of self-limited febrile syndrome in the Mediterranean basin in summer and autumn. Toscana virus can also cause meningitis and meningoencephalitis. In Spain, Toscana, Granada, Naples, Sicily, Arbia and Arrabida-like viruses have been detected. The almost widespread distribution of Phlebotomus genus vectors, and especially Phlebotomus perniciosus, in which several of these viruses have been detected, makes it very likely that there will be regular human infections in our country, with this risk considered moderate for Toscana virus and low for the other ones, in areas with the highest vector activity. Most of the infections would be undiagnosed, while only Toscana virus would have a greater impact due to the potential severity of the illness.
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- 2020
38. Clinical Outcomes of a Zika Virus Mother-Child Pair Cohort in Spain
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Carlota Rodó, Ana Vázquez, Elena Sulleiro, María Paz Sánchez-Seco, Alfredo García-Alix, Claire Thorne, Elena Carreras, Ainara Salazar, Milagros García López-Hortelano, Fernando de Ory, Marie Antoinette Frick, Marta Cabrera-Lafuente, Anna Suy, Thomas Byrne, Antoni Soriano-Arandes, José Tomás Ramos-Amador, Pere Soler-Palacín, María Del Carmen Viñuela, Elisa Ruiz-Burga, María José Mellado-Peña, Resurrección Reques-Cosme, Daniel Blázquez-Gamero, Luis Prieto, Mar Santos, María de la Calle, Carlo Giaquinto, Borja Guarch-Ibáñez, Iciar Olabarrieta, Andrea Martín-Nalda, A E Ades, María Espiau, Eva Dueñas, Juan Navarro-Morón, E. Antolin, European Union, UAM. Departamento de Obstetricia y Ginecología, UAM. Departamento de Pediatría, and Instituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ)
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Microbiology (medical) ,Pediatrics ,medicine.medical_specialty ,Microcephaly ,Referral ,Medicina ,lcsh:Medicine ,Asymptomatic ,Article ,Zika virus ,Adverse outcome ,03 medical and health sciences ,0302 clinical medicine ,Congenital infection ,030225 pediatrics ,Immunology and Allergy ,Medicine ,030212 general & internal medicine ,Molecular Biology ,Pregnancy ,Arboviruses ,General Immunology and Microbiology ,biology ,business.industry ,Incidence (epidemiology) ,lcsh:R ,medicine.disease ,biology.organism_classification ,3. Good health ,Infectious Diseases ,Cohort ,medicine.symptom ,business ,Cohort study - Abstract
Background: Zika virus (ZIKV) infection has been associated with congenital microcephaly and other neurodevelopmental abnormalities. There is little published research on the effect of maternal ZIKV infection in a non-endemic European region. We aimed to describe the outcomes of pregnant travelers diagnosed as ZIKV-infected in Spain, and their exposed children. Methods: This prospective observational cohort study of nine referral hospitals enrolled pregnant women (PW) who travelled to endemic areas during their pregnancy or the two previous months, or those whose sexual partners visited endemic areas in the previous 6 months. Infants of ZIKV-infected mothers were followed for about two years. Results: ZIKV infection was diagnosed in 163 PW, 112 (70%) were asymptomatic and 24 (14.7%) were confirmed cases. Among 143 infants, 14 (9.8%) had adverse outcomes during follow-up, three had a congenital Zika syndrome (CZS), and 11 other potential Zika-related outcomes. The overall incidence of CZS was 2.1% (95%CI: 0.4&ndash, 6.0%), but among infants born to ZIKV-confirmed mothers, this increased to 15.8% (95%CI: 3.4&ndash, 39.6%). Conclusions: A nearly 10% overall risk of neurologic and hearing adverse outcomes was found in ZIKV-exposed children born to a ZIKV-infected traveler PW. Longer-term follow-up of these children is needed to assess whether there are any later-onset manifestations.
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- 2020
39. Cytotoxic cell populations developed during treatment with tyrosine kinase inhibitors protect autologous CD4+ T cells from HIV-1 infection
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Alejandro Luna, Fernando de Ory, Lorena Vigón, Vicente Planelles, Pilar Pérez-Romero, Montserrat Plana, Valentín García-Gutiérrez, Elena Mateos, Mayte Coiras, Juan Luis Steegmann, Virginia Sandonis, Sara Rodríguez-Mora, Núria Climent, José Alcamí, Guiomar Bautista, María Rosa López-Huertas, Ministerio de Economía y Competitividad (España), Red de Investigación Cooperativa en Investigación en Sida, European Regional Development Fund, and National Institutes of Health (Estados Unidos)
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0301 basic medicine ,Adult ,CD4-Positive T-Lymphocytes ,Male ,HIV Infections ,Biochemistry ,Antiviral Agents ,Article ,03 medical and health sciences ,0302 clinical medicine ,Viral reservoir ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,medicine ,Cytotoxic T cell ,Humans ,Protein Kinase Inhibitors ,Aged ,Pharmacology ,Aged, 80 and over ,Innate immune system ,business.industry ,Chronic myeloid leukemia ,Myeloid leukemia ,Src tyrosine kinases ,Middle Aged ,In vitro ,SAMHD1 ,Dasatinib ,030104 developmental biology ,Cytoprotection ,030220 oncology & carcinogenesis ,Cancer research ,HIV-1 ,Female ,business ,Tyrosine kinase ,Ex vivo ,medicine.drug - Abstract
Factor de impacto: 5,858 Q1 Tyrosine kinase inhibitors (TKIs) are successfully used in clinic to treat chronic myeloid leukemia (CML). Our group previously described that CD4+ T cells from patients with CML on treatment with TKIs such as dasatinib were resistant to HIV-1 infection ex vivo. The main mechanism for this antiviral activity was primarily based on the inhibition of SAMHD1 phosphorylation, which preserves the activity against HIV-1 of this innate immune factor. Approximately 50% CML patients who achieved a deep molecular response (DMR) may safely withdraw TKI treatment without molecular recurrence. Therefore, it has been speculated that TKIs may induce a potent antileukemic response that is maintained in most patients even one year after treatment interruption (TI). Subsequent to in vitro T-cell activation, we observed that SAMHD1 was phosphorylated in CD4+ T cells from CML patients who withdrew TKI treatment more than one year earlier, which indicated that these cells were now susceptible to HIV-1 infection. Importantly, these patients were seronegative for HIV-1 and seropositive for cytomegalovirus (CMV), but without CMV viremia. Although activated CD4+ T cells from CML patients on TI were apparently permissive to HIV-1 infection ex vivo, the frequency of proviral integration was reduced more than 12-fold on average when these cells were infected ex vivo in comparison with cells isolated from untreated, healthy donors. This reduced susceptibility to infection could be related to an enhanced NK-dependent cytotoxic activity, which was increased 8-fold on average when CD4+ T cells were infected ex vivo with HIV-1 in the presence of autologous NK cells. Enhanced cytotoxic activity was also observed in CD8 + T cells from these patients, which showed 8-fold increased expression of TCRγδ and more than 18-fold increased production of IFNγ upon activation with CMV peptides. In conclusion, treatment with TKIs induced a potent antileukemic response that may also have antiviral effects against HIV-1 and CMV, suggesting that transient use of TKIs in HIV-infected patients could develop a sustained antiviral response that would potentially interfere with HIV-1 reservoir dynamics. This work was supported by NIH grant R01AI143567; the Spanish Ministry of Economy and Competitiveness (SAF2016-78480-R); the Spanish AIDS Research Network RD16CIII/0002/0001 that is included in Acción Estratégica en Salud, Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica 2016-2020, Instituto de Salud Carlos III, European Region Development Fund (ERDF). The work of María Rosa López-Huertas and Sara Rodríguez-Mora is financed by NIH grant R01AI143567. The work of Lorena Vigón is supported by a pre-doctoral grant from Instituto de Salud Carlos III (FIS PI16CIII/00034-ISCIII-FEDER). The work of Elena Mateos is supported by the Spanish Ministry of Economy and Competitiveness SAF2016-78480-R. Sí
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- 2020
40. Crimean-Congo haemorrhagic fever (CCHF) virus-specific antibody detection in blood donors, Castile-León, Spain, summer 2017 and 2018
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Nuria Leralta, María Paz Sánchez Seco, Roger Hewson, Montserrat Alonso Sardón, Fernando de Ory Manchón, Ana Jiménez del Bianco, María Belén Vicente Santiago, Rufino Alamo-Sanz, Sonia Pérez González, Ana Isabel Negredo, Moncef Belhassen-García, Isabel Bas, Juan Luis Muñoz Bellido, Lydia Blanco Peris, María Carmen Vieira Lista, Pedro Fernández Soto, Lía Monsalve Arteaga, Antonio Muro, Instituto de Salud Carlos III, and Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
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0301 basic medicine ,Male ,Veterinary medicine ,Epidemiology ,Blood Donors ,emerging diseases ,Antibodies, Viral ,viral haemorrhagic fever ,Laboratory ,0302 clinical medicine ,Ticks ,Seroepidemiologic Studies ,Tick-borne diseases ,030212 general & internal medicine ,Animal Husbandry ,Crimean-Congo haemorrhagic fever ,Tick-borne disease ,biology ,Emerging diseases ,Middle Aged ,Specific antibody ,Hemorrhagic Fever Virus, Crimean-Congo ,epidemiology ,Female ,Antibody ,medicine.symptom ,Adult ,medicine.medical_specialty ,Blood donor ,tick-borne diseases ,re-emerging diseases ,Enzyme-Linked Immunosorbent Assay ,Asymptomatic ,Virus ,03 medical and health sciences ,Virology ,CCHF ,medicine ,Animals ,Humans ,CCHF VIRUS ,blood donor ,Viral haemorrhagic fever ,Aged ,Re-emerging diseases ,business.industry ,Research ,Public Health, Environmental and Occupational Health ,medicine.disease ,030104 developmental biology ,Cross-Sectional Studies ,Spain ,biology.protein ,Hemorrhagic Fever, Crimean ,business ,laboratory - Abstract
Background Crimean-Congo haemorrhagic fever virus (CCHFV) is considered an emerging or even a probable re-emerging pathogen in southern Europe. Presence of this virus had been reported previously in Spain in 2010. Aim We aimed to evaluate the potential circulation of CCHFV in western Spain with a serosurvey in asymptomatic adults (blood donors). Methods During 2017 and 2018, we conducted a CCHFV serosurvey in randomly selected asymptomatic blood donors from western Spain. Three assays using specific IgG antibodies against CCHFV were performed: the VectoCrimea ELISA test, an in-house ELISA and indirect immunofluorescence (EuroImmun) test with glycoprotein and nucleoprotein. Results A total of 516 blood donors participated in this cross-sectional study. The majority of the study participants were male (68.4%), and the mean age was 46.3 years. Most of the participants came from rural areas (86.8%) and 68.6% had contact with animals and 20.9% had animal husbandry practices. One in five participants (109/516, 21.1%) were engaged in at-risk professional activities such as agriculture and shepherding, slaughtering, hunting, veterinary and healthcare work (mainly nursing staff and laboratory technicians). A total of 15.3% of the participants were bitten by ticks in the days or months before the date of sampling. We detected anti-CCHFV IgG antibodies with two diagnostic assays in three of the 516 individuals and with one diagnostic assay in six of the 516 individuals. Conclusion Seroprevalence of CCHFV was between 0.58% and 1.16% in Castile-León, Spain. This is the first study in western Spain that showed circulation of CCHFV in healthy people.
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- 2020
41. Evaluation of the LIAISON XL Zika Capture IgM II for the Diagnosis of Zika Virus Infections
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María Paz Sánchez-Seco, Fernando de Ory, Ana Vázquez, Jesús de la Fuente, Pilar Balfagón, Teodora Minguito, Mayte Pérez-Olmeda, María Ángeles Murillo, and Instituto de Salud Carlos III
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0301 basic medicine ,viruses ,030106 microbiology ,lcsh:QR1-502 ,Enzyme-Linked Immunosorbent Assay ,Dengue virus ,medicine.disease_cause ,Antibodies, Viral ,Rubella ,Measles ,Sensitivity and Specificity ,Virus ,lcsh:Microbiology ,Article ,Enzyme immunoassay ,Dengue fever ,Zika virus ,Measles virus ,03 medical and health sciences ,Virology ,medicine ,Humans ,False Positive Reactions ,Serologic Tests ,Chemiluminescent immunoassay ,Chikungunya ,chemiluminescent immunoassay ,dengue viruses ,chikungunya virus ,biology ,business.industry ,Zika Virus Infection ,virus diseases ,Zika Virus ,medicine.disease ,biology.organism_classification ,enzyme immunoassay ,030104 developmental biology ,Infectious Diseases ,Immunoglobulin M ,Virus Diseases ,Dengue viruses ,business ,Chikungunya virus - Abstract
The aim of this study is to evaluate the performance characteristics of the LIAISON XL Zika Capture IgM II. For this purpose we tested 128 samples obtained from recent infections caused by the Zika (ZIKV, 74 samples), dengue (DENV, 10 samples), chikungunya (CHIK V, 11 samples), rubella (RUBV, 10 samples) and measles (MeV, 10 samples) viruses, as well as human parvovirus B19 (HPVB19, 13 samples). The results of the assay under evaluation are compared with those obtained from an indirect immunofluorescence (IIF) assay, and the discrepancies are resolved by considering other laboratory results (PCR and a plaque-reduction neutralization test). The LIAISON showed excellent sensitivity (100%). The specificity (91.25%) was hampered by some false-positive results in recent dengue virus, chikungunya virus, measles virus and human parvovirus B19 infections. The method evaluated is adequate, but the low specificity makes it necessary to consider the clinical and epidemiological contexts of patients, as well as other laboratory results.
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- 2020
42. From the sky and atmosphere of the Canarias: the creation of the Instituto de Astrofísica de Canarias
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Fernando de Ory Ajamil
- Subjects
observatorio meteorológico ,Meteorological observatory ,media_common.quotation_subject ,astrofísico ,General Medicine ,Art ,Izaña ,Humanities ,Teide ,media_common - Abstract
Desde el comienzo del siglo xvii son conocidas las excepcionales condiciones del cielo y la atmósfera en las cumbres del archipiélago canario. En un tiempo muy posterior, a mediados del siglo xx, el actual Instituto de Astrofísica de Canarias (IAC) surgió en el Observatorio Meteorológico de Izaña (OMI). En sus dependencias se alojaron y trabajaron los primeros investigadores del IAC. El primer edificio del Observatorio Astronómico del Teide (OAT) y el suministro eléctrico para su funcionamiento fueron sufragados por el OMI. Gran parte del personal del OMI trabajó y colaboró en las primeras observaciones del OAT. La larga serie de observaciones climatológicas del OMI constituyó la base y el fundamento sobre el que se justificó la idoneidad del emplazamiento para la investigación astronómica. Así sucedió en los primeros años, posteriores a 1959, cuando se creó oficialmente el OAT. Since the beginning of the seventeenth century the exceptional conditions of the sky and the atmosphere on the summits of the Canary Islands archipelago have been known. Much later time, in the middle of the 20th century, the current Instituto de Astrofísica de Canarias (IAC) emerged at the Meteorological Observatory of Izaña (OMI). The first researchers of the IAC stayed and worked in its installations. The first building of the Observatorio Astronómico del Teide (OAT) and the electricity supply for its operation were financed by OMI. Much of the OMI staff worked and collaborated on the first observations of the OAT. The long series of IMO climate observations provided the basis and basis on which the suitability of the site for astronomical research was justified. This was the case in the early years, after 1959, when the OAT was officially established.
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- 2020
43. Imported Human West Nile Virus Lineage 2 Infection in Spain: Neurological and Gastrointestinal Complications
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Laura Herrero, Alejandro Algora, Francisca Molero, Fernando de Ory, María Velasco, Carolina Campelo, Teodora Minguito, María Paz Sánchez-Seco, Octavio J Salmerón Béliz, Ana Vázquez, Oriol Martin, Mª Carmen Campos, and Instituto de Salud Carlos III
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0301 basic medicine ,Male ,Gastrointestinal Diseases ,viruses ,lcsh:QR1-502 ,Viral Nonstructural Proteins ,Antibodies, Viral ,lcsh:Microbiology ,Serology ,Disease Outbreaks ,0302 clinical medicine ,Communicable Diseases, Imported ,west nile virus lineage 2 ,Digestive complications ,Phylogeny ,Communication ,virus diseases ,West Nile virus lineage 2 ,Middle Aged ,Diarrhea ,Titer ,Infectious Diseases ,RNA, Viral ,Antibody ,medicine.symptom ,West Nile virus ,Human ,030231 tropical medicine ,Neurological symptoms ,neurological symptoms ,Biology ,Virus ,03 medical and health sciences ,Virology ,medicine ,spain ,Humans ,human ,Romania ,digestive complications ,medicine.disease ,Antibodies, Neutralizing ,030104 developmental biology ,Spain ,Cholecystitis ,biology.protein ,Pancreatitis ,Nervous System Diseases ,Complication ,West Nile Fever - Abstract
We report the first human case of West Nile virus (WNV) lineage 2 infection imported to Spain by a traveler returning from Romania. Serum, cerebrospinal fluid and urine samples were analyzed and West Nile virus infection was identified by PCR and serological tests. The patient developed fever, diarrhea and neurological symptoms, accompanied by mild pancreatitis, described previously in very few cases as a complication of WNV infection and by alithiasic cholecystitis. Viral RNA was detected in urine until 30 days after the onset of symptoms and neutralizing antibodies were detected at very low titers. The phylogenetic analysis in a fragment of the NS5 gene of the virus showed a homology with sequences from WNV lineage 2 belonging to the monophyletic Central/Southern European group. This work was partially funded by the Instituto de Salud Carlos III Projects “PI14CIII/00014” and “PI19CIII_00014”. Sí
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- 2020
44. Initial experience with imported Zika virus infection in Spain
- Author
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Marta Arsuaga, Fernando de Ory, Jose R. Arribas, Ana Vázquez, Silvia García-Bujalance, Dolores Montero, Fernando de la Calle-Prieto, Paz Sánchez Seco, Elena Trigo, María de la Calle, E. Antolin, and Marta Díaz-Menéndez
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Microbiology (medical) ,0301 basic medicine ,medicine.medical_specialty ,Pediatrics ,biology ,business.industry ,030231 tropical medicine ,030106 microbiology ,Outbreak ,Endemic area ,Diagnostic test ,Disease ,biology.organism_classification ,Virology ,Zika virus ,03 medical and health sciences ,0302 clinical medicine ,Tropical medicine ,Cohort ,medicine ,030212 general & internal medicine ,business - Abstract
Introduction A considerable increase of imported Zika virus (ZIKV) infection has been reported in Europe in the last year. This is the result of the large outbreak of the disease in the Americas, along with the increase in the numbers of travellers and immigrants arriving from ZIKV endemic areas. Methods A descriptive study was conducted in the Tropical Medicine Unit of Hospital La Paz-Carlos III in Madrid on travellers returning from an endemic area for ZIKV from January to April 2016. Demographic, clinical and microbiological data were analyzed. Results A total of 185 patients were screened for ZIKV (59.9% women, median age of 37.7 ± 10.3 years). Main purpose of the travel was tourism to Colombia, Brazil, and Mexico. Just under three-quarters (73%) were symptomatic, mostly with fever and headache. A total of 13 patients (7% of those screened) were diagnosed with ZIKV infections, of which four of them were pregnant. All of them were symptomatic patients, the majority immigrants, and mainly from Colombia. Diagnostic tests were based on positive neutralization antibodies (8 cases, 61.6%) and a positive RT-PCR in different organic fluids (7 cases, 53.8%) The four infected pregnant women underwent a neurosonography every 3 weeks, and no alterations were detected. RT-PCR in amniotic fluid was performed in three of them, with negative results. One of the children has already been born healthy. Conclusions Our cases series represents the largest cohort of imported ZIKV to Spain described until now. Clinicians must increase awareness about the progression of the ZIKV outbreak and the affected areas so that they can include Zika virus infection in their differential diagnosis for travellers from those areas.
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- 2018
45. Valoración de un kit comercializado de RT-PCR para el diagnóstico de la infección por el virus del sarampión
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Fernando de Ory, Aurora Fernández-García, Juan Carlos Sanz, and Juan Emilio Echevarría
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Microbiology (medical) ,business.industry ,Medicine ,business - Published
- 2021
46. Valuation of a commercialized RT-PCR kit for the diagnosis of infection caused by the measles virus
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Aurora Fernández-García, Juan Carlos Sanz, Juan Emilio Echevarría, and Fernando de Ory
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Valuation (logic) ,Measles virus ,Real-time polymerase chain reaction ,biology ,business.industry ,Medicine ,business ,biology.organism_classification ,Virology - Published
- 2021
47. Diagnóstico rápido en serología
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Fernando de Ory and Isabel García-Bermejo
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Transmission (medicine) ,business.industry ,030106 microbiology ,Human immunodeficiency virus (HIV) ,Target population ,medicine.disease_cause ,medicine.disease ,Serology ,03 medical and health sciences ,Immunology ,medicine ,Clinical significance ,Syphilis ,Intensive care medicine ,business - Abstract
Serological diagnosis of acute phase infections implies the detection of IgM specific response, an effective marker of primary infection, but with less clinical significance in reactivations or reinfections. The aim of this article is to provide an updated view of the rapid diagnosis in serology by detecting the IgM isotype and reviewing its applications and limitations. Point-of-care (PoC) tests are analyzed. PoC tests are used in geographical areas where traditional tests are not available, as well as in other circumstances where their use brings the diagnosis directly to the target population. Likewise, their use reduces the response time between taking the sample and the diagnosis, making it easier to make clinical decisions. PoC assays have proven cost-effective, especially in preventing vertical transmission of syphilis and HIV infection.
- Published
- 2017
48. Rapid diagnosis in serology
- Author
-
Isabel García-Bermejo and Fernando de Ory
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Time Factors ,business.industry ,Transmission (medicine) ,030106 microbiology ,Human immunodeficiency virus (HIV) ,Target population ,Infections ,medicine.disease ,medicine.disease_cause ,Serology ,Specific igm ,03 medical and health sciences ,Immunology ,medicine ,Humans ,Serologic Tests ,Syphilis ,Clinical significance ,Intensive care medicine ,business - Abstract
Serological diagnosis of acute phase infections implies the detection of IgM specific response, an effective marker of primary infection, but with less clinical significance in reactivations or reinfections. The aim of this article is to provide an updated view of the rapid diagnosis in serology by detecting the IgM isotype and reviewing its applications and limitations. Point-of-care (PoC) tests are analyzed. PoC tests are used in geographical areas where traditional tests are not available, as well as in other circumstances where their use brings the diagnosis directly to the target population. Likewise, their use reduces the response time between taking the sample and the diagnosis, making it easier to make clinical decisions. PoC assays have proven cost-effective, especially in preventing vertical transmission of syphilis and HIV infection.
- Published
- 2017
49. Serological diagnosis of mumps: Value of the titration of specific IgG
- Author
-
Fernando de Ory, Juan Emilio Echevarría, Juan Carlos Sanz, Luis García-Comas, Belén Ramos, and Aurora Arjones Fernández
- Subjects
education.field_of_study ,Saliva ,030505 public health ,business.industry ,Population ,Specific igg ,medicine.disease ,Serum samples ,Measles ,Serology ,03 medical and health sciences ,Rubella vaccine ,0302 clinical medicine ,Immunology ,medicine ,030212 general & internal medicine ,0305 other medical science ,education ,business ,medicine.drug - Abstract
Introduction The aim of this study was to evaluate a cut-off point of the titration of IgG by ELISA in the diagnosis of mumps. Methods A study was made of serum samples from 85 mumps cases (confirmed by PCR in saliva) and 2351 controls of the general population of the Region of Madrid. Results The IgM detection was positive in 21 cases (sensitivity of 24.7%). The best cut-off point corresponded to IgG titres ≥ 4900 (sensitivity of 64.7% and specificity of 86.1%). Among 42 patients immunised with at least one dose of measles mumps, rubella vaccine IgM was detected in 4 cases. However, the detection of IgG ≥ 4900 was positive in 29 (sensitivity of 69.0%). Conclusions An IgG result of ≥4900 was almost 5 times more probable in a patient with mumps than in a non-infected patient. The detection of high titres of IgG against mumps could improve the IgM results in vaccinated people.
- Published
- 2018
50. Diagnóstico serológico de parotiditis epidémica: valor de la titulación de IgG específica
- Author
-
Juan Emilio Echevarría, Luis García-Comas, Aurora Arjones Fernández, Belén Ramos, Juan Carlos Sanz, and Fernando de Ory
- Subjects
0301 basic medicine ,Microbiology (medical) ,03 medical and health sciences ,0302 clinical medicine ,030106 microbiology ,030212 general & internal medicine - Abstract
Resumen Introduccion El objetivo del estudio fue determinar un punto de corte de la titulacion de IgG mediante ELISA en el diagnostico de parotiditis. Metodos Se estudiaron sueros de 85 casos de parotiditis (confirmados por PCR en saliva) y de 2.351 controles de la poblacion de la Comunidad de Madrid. Resultados La deteccion de IgM fue positiva en 21 casos (sensibilidad del 24,7). El mejor punto de corte de IgG correspondia a titulos ≥ 4.900 (sensibilidad del 64,7% y especificidad del 86,1%). De 42 pacientes vacunados con ≥ 1 dosis de triple virica se detecto IgM en 4, mientras que la deteccion de IgG ≥ 4.900 fue positiva en 29 (sensibilidad del 69,0%). Conclusiones Un resultado de IgG ≥ 4.900 fue casi 5 veces mas probable en un paciente con parotiditis que en otro sujeto no infectado. La deteccion de titulos elevados de IgG frente a parotiditis puede mejorar el rendimiento diagnostico de la IgM en vacunados.
- Published
- 2018
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