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Evaluation of the LIAISON XL Zika Capture IgM II for the Diagnosis of Zika Virus Infections
- Source :
- Repisalud, Instituto de Salud Carlos III (ISCIII), Viruses, Viruses, Vol 12, Iss 1, p 69 (2020), Volume 12, Issue 1
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute (MDPI), 2020.
-
Abstract
- The aim of this study is to evaluate the performance characteristics of the LIAISON XL Zika Capture IgM II. For this purpose we tested 128 samples obtained from recent infections caused by the Zika (ZIKV<br />74 samples), dengue (DENV<br />10 samples), chikungunya (CHIK V<br />11 samples), rubella (RUBV<br />10 samples) and measles (MeV<br />10 samples) viruses, as well as human parvovirus B19 (HPVB19<br />13 samples). The results of the assay under evaluation are compared with those obtained from an indirect immunofluorescence (IIF) assay, and the discrepancies are resolved by considering other laboratory results (PCR and a plaque-reduction neutralization test). The LIAISON showed excellent sensitivity (100%). The specificity (91.25%) was hampered by some false-positive results in recent dengue virus, chikungunya virus, measles virus and human parvovirus B19 infections. The method evaluated is adequate, but the low specificity makes it necessary to consider the clinical and epidemiological contexts of patients, as well as other laboratory results.
- Subjects :
- 0301 basic medicine
viruses
030106 microbiology
lcsh:QR1-502
Enzyme-Linked Immunosorbent Assay
Dengue virus
medicine.disease_cause
Antibodies, Viral
Rubella
Measles
Sensitivity and Specificity
Virus
lcsh:Microbiology
Article
Enzyme immunoassay
Dengue fever
Zika virus
Measles virus
03 medical and health sciences
Virology
medicine
Humans
False Positive Reactions
Serologic Tests
Chemiluminescent immunoassay
Chikungunya
chemiluminescent immunoassay
dengue viruses
chikungunya virus
biology
business.industry
Zika Virus Infection
virus diseases
Zika Virus
medicine.disease
biology.organism_classification
enzyme immunoassay
030104 developmental biology
Infectious Diseases
Immunoglobulin M
Virus Diseases
Dengue viruses
business
Chikungunya virus
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repisalud, Instituto de Salud Carlos III (ISCIII), Viruses, Viruses, Vol 12, Iss 1, p 69 (2020), Volume 12, Issue 1
- Accession number :
- edsair.doi.dedup.....a5ece944d6ef0a3ab13e318a1052b3d0