31 results on '"Fernanda de Carvalho-Niebel"'
Search Results
2. Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells
- Author
-
Audrey Kelner, Nuno Leitão, Mireille Chabaud, Myriam Charpentier, and Fernanda de Carvalho-Niebel
- Subjects
Medicago truncatula ,Arabidopsis thaliana ,root symbiosis ,root hairs ,root elongation zone ,calcium ,Plant culture ,SB1-1110 - Abstract
Spatiotemporal changes in cellular calcium (Ca2+) concentrations are essential for signal transduction in a wide range of plant cellular processes. In legumes, nuclear and perinuclear-localized Ca2+ oscillations have emerged as key signatures preceding downstream symbiotic signaling responses. Förster resonance energy transfer (FRET) yellow-based Ca2+ cameleon probes have been successfully exploited to measure the spatiotemporal dynamics of symbiotic Ca2+ signaling in legumes. Although providing cellular resolution, these sensors were restricted to measuring Ca2+ changes in single subcellular compartments. In this study, we have explored the potential of single fluorescent protein-based Ca2+ sensors, the GECOs, for multicolor and simultaneous imaging of the spatiotemporal dynamics of cytoplasmic and nuclear Ca2+ signaling in root cells. Single and dual fluorescence nuclear and cytoplasmic-localized GECOs expressed in transgenic Medicago truncatula roots and Arabidopsis thaliana were used to successfully monitor Ca2+ responses to microbial biotic and abiotic elicitors. In M. truncatula, we demonstrate that GECOs detect symbiosis-related Ca2+ spiking variations with higher sensitivity than the yellow FRET-based sensors previously used. Additionally, in both M. truncatula and A. thaliana, the dual sensor is now able to resolve in a single root cell the coordinated spatiotemporal dynamics of nuclear and cytoplasmic Ca2+ signaling in vivo. The GECO-based sensors presented here therefore represent powerful tools to monitor Ca2+ signaling dynamics in vivo in response to different stimuli in multi-subcellular compartments of plant cells.
- Published
- 2018
- Full Text
- View/download PDF
3. api, A Novel Medicago truncatula Symbiotic Mutant Impaired in Nodule Primordium Invasion
- Author
-
Alice Teillet, Joseph Garcia, Françoise de Billy, Michèle Gherardi, Thierry Huguet, David G. Barker, Fernanda de Carvalho-Niebel, and Etienne-Pascal Journet
- Subjects
MtENOD ,nitrogen fixation ,short root hair ,Microbiology ,QR1-502 ,Botany ,QK1-989 - Abstract
Genetic approaches have proved to be extremely useful in dissecting the complex nitrogen-fixing Rhizobium–legume endosymbiotic association. Here we describe a novel Medicago truncatula mutant called api, whose primary phenotype is the blockage of rhizobial infection just prior to nodule primordium invasion, leading to the formation of large infection pockets within the cortex of noninvaded root outgrowths. The mutant api originally was identified as a double symbiotic mutant associated with a new allele (nip-3) of the NIP/LATD gene, following the screening of an ethylmethane sulphonate–mutagenized population. Detailed characterization of the segregating single api mutant showed that rhizobial infection is also defective at the earlier stage of infection thread (IT) initiation in root hairs, as well as later during IT growth in the small percentage of nodules which overcome the primordium invasion block. Neither modulating ethylene biosynthesis (with L-α-(2-aminoethoxyvinylglycine or 1-aminocyclopropane-1-carboxylic acid) nor reducing ethylene sensitivity in a skl genetic background alters the basic api phenotype, suggesting that API function is not closely linked to ethylene metabolism or signaling. Genetic mapping places the API gene on the upper arm of the M. truncatula linkage group 4, and epistasis analyses show that API functions downstream of BIT1/ERN1 and LIN and upstream of NIP/LATD and the DNF genes.
- Published
- 2008
- Full Text
- View/download PDF
4. MtENOD11 Gene Activation During Rhizobial Infection and Mycorrhizal Arbuscule Development Requires a Common AT-Rich-Containing Regulatory Sequence
- Author
-
Aurélien Boisson-Dernier, Andry Andriankaja, Mireille Chabaud, Andreas Niebel, Etienne-P. Journet, David G. Barker, and Fernanda de Carvalho-Niebel
- Subjects
AATAA motif ,Agrobacterium rhizogenes ,Glomus ,Meloidogyne ,nodFnodL ,Microbiology ,QR1-502 ,Botany ,QK1-989 - Abstract
The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont. To identify the regulatory sequences involved in symbiosis-related MtENOD11 expression, a series of promoter deletions driving the β-glucuronidase reporter gene were analyzed in transgenic M. truncatula roots. These studies have revealed that distinct regulatory regions are involved in infection-related MtENOD11 expression compared with preinfection (Nod factor-mediated) expression. In particular, the 257-bp promoter sequence immediately upstream from the start codon is sufficient for infection-related expression, but is unable to drive gene transcription in response to the Nod factor elicitor. This truncated promoter is also sufficient to confer MtENOD11 expression during both the arbuscular mycorrhizal (AM) association and the parasitic interaction with root-knot nematodes. Site-directed mutagenesis further showed that a previously identified nodule-specific AT-rich motif is required for high-level MtENOD11 expression during S. meliloti infection as well as during AM colonization. However, mutation of this motif does not affect gene expression associated with nematode-feeding sites. Taken together, these results suggest a close link between regulatory mechanisms controlling transcriptional early nodulin gene activation during both rhizobial and AM root endosymbioses.
- Published
- 2005
- Full Text
- View/download PDF
5. Transcription reprogramming during root nodule development in Medicago truncatula.
- Author
-
Sandra Moreau, Marion Verdenaud, Thomas Ott, Sébastien Letort, Françoise de Billy, Andreas Niebel, Jérôme Gouzy, Fernanda de Carvalho-Niebel, and Pascal Gamas
- Subjects
Medicine ,Science - Abstract
Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are shared with other plant developmental programs also remains an open question. Here detailed microarray analyses have been used to study the transcriptome of root nodules induced by either wild type or mutant strains of Sinorhizobium meliloti. In this way we have defined eight major activation patterns in nodules and identified associated potential regulatory genes. We have shown that transcription reprogramming during consecutive stages of nodule differentiation occurs in four major phases, respectively associated with (i) early signalling events and/or bacterial infection; plant cell differentiation that is either (ii) independent or (iii) dependent on bacteroid differentiation; (iv) nitrogen fixation. Differential expression of several genes involved in cytokinin biosynthesis was observed in early symbiotic nodule zones, suggesting that cytokinin levels are actively controlled in this region. Taking advantage of databases recently developed for M. truncatula, we identified a small subset of gene expression regulators that were exclusively or predominantly expressed in nodules, whereas most other regulators were also activated under other conditions, and notably in response to abiotic or biotic stresses. We found evidence suggesting the activation of the jasmonate pathway in both wild type and mutant nodules, thus raising questions about the role of jasmonate during nodule development. Finally, quantitative RT-PCR was used to analyse the expression of a series of nodule regulator and marker genes at early symbiotic stages in roots and allowed us to distinguish several early stages of gene expression activation or repression.
- Published
- 2011
- Full Text
- View/download PDF
6. Developmental Modulation of Root Cell Wall Architecture Confers Resistance to an Oomycete Pathogen
- Author
-
Abhishek Chatterjee, Justine Toulotte, Edouard Evangelisti, Etienne-Pascal Journet, Frédéric Debellé, Aleksandr Gavrin, Thomas Rey, Thomas A. Torode, Siobhan A. Braybrook, Hiroki Takagi, Sebastian Schornack, Ryohei Terauchi, David Rengel, Varodom Charoensawan, Fernanda de Carvalho-Niebel, J L Kaplan, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Apollo-University Of Cambridge Repository, University of Cambridge [UK] (CAM), Iwate Biotechnology Research Center (IBRC), Mahidol University [Bangkok], Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AGroécologie, Innovations, teRritoires (AGIR), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of California [Los Angeles] (UCLA), University of California, ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Gavrin, Aleksandr [0000-0003-0179-8491], Schornack, Sebastian [0000-0002-7836-5881], and Apollo - University of Cambridge Repository
- Subjects
Phytophthora ,0301 basic medicine ,disease resistance ,[SDV]Life Sciences [q-bio] ,Mutant ,Rhizobia ,Plant Roots ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell wall ,QH301 ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Wall ,Gene Expression Regulation, Plant ,Medicago truncatula ,susceptibility gene ,Endomembrane system ,Secretion ,Symbiosis ,Actin ,ComputingMilieux_MISCELLANEOUS ,Plant Diseases ,Plant Proteins ,Oomycete ,biology ,root endosymbiosis ,Plants, Genetically Modified ,biology.organism_classification ,SCAR/WAVE ,Actins ,Cell biology ,Xyloglucan ,030104 developmental biology ,chemistry ,Host-Pathogen Interactions ,Mutation ,susceptibility gen ,General Agricultural and Biological Sciences ,030217 neurology & neurosurgery ,Rhizobium - Abstract
Summary The cell wall is the primary interface between plant cells and their immediate environment and must balance multiple functionalities, including the regulation of growth, the entry of beneficial microbes, and protection against pathogens. Here, we demonstrate how API, a SCAR2 protein component of the SCAR/WAVE complex, controls the root cell wall architecture important for pathogenic oomycete and symbiotic bacterial interactions in legumes. A mutation in API results in root resistance to the pathogen Phytophthora palmivora and colonization defects by symbiotic rhizobia. Although api mutant plants do not exhibit significant overall growth and development defects, their root cells display delayed actin and endomembrane trafficking dynamics and selectively secrete less of the cell wall polysaccharide xyloglucan. Changes associated with a loss of API establish a cell wall architecture with altered biochemical properties that hinder P. palmivora infection progress. Thus, developmental stage-dependent modifications of the cell wall, driven by SCAR/WAVE, are important in balancing cell wall developmental functions and microbial invasion., Graphical Abstract, Highlights • The SCAR protein API controls actin and endomembrane trafficking dynamics • SCAR proteins of several plant species can support symbiosis and pathogen infection • A mutation in API affects specific biochemical properties of plant cell walls • An altered wall architecture results in root resistance to Phytophthora palmivora, Subtly altered plant cell walls can be decisive for disease. Gavrin et al. show that the Medicago SCAR/WAVE complex protein API controls actin cytoskeleton dynamics in roots. Local changes associated with a loss of API establish a cell wall architecture with altered biochemical properties that hinder infection progress by an oomycete pathogen.
- Published
- 2020
7. A simple Agrobacterium tumefaciens-mediated transformation method for rapid transgene expression in Medicago truncatula root hairs
- Author
-
Mireille Chabaud, Joëlle Fournier, Fernanda de Carvalho-Niebel, Céline Remblière, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Plant Health & Environment Division of INRA : ANR project RIPOSTE, ANR-14-CE19-0024, Marie Slodowska-Curie Actions of the European Commission : CIG-334036, EMBO grant (Long-Term Fellowship) : ALTF 1627-2011, EMBO grant (Marie Curie Actions EMBOCOFUND) : GA-2010-267146, French Laboratory of Excellence project 'TULIP' : ANR-10-LABX-41, European Project: 336808,EC:FP7:ERC,ERC-2013-StG,VARIWHIM(2013), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-14-CE35-0007,COME-IN,Cell-specific reprogramming of legume roots for endosymbiotic infection(2014), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)
- Subjects
Fluorescent protein sub-cellular localization ,0106 biological sciences ,0301 basic medicine ,Agrobacterium ,Transgene ,Transient transformation ,Horticulture ,Root hair ,01 natural sciences ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Medicago truncatula ,Botany ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Transgene expression ,biology ,fungi ,food and beverages ,Plant physiology ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Agrobacterium tumefaciens ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Fusion protein ,Cell biology ,Transformation (genetics) ,030104 developmental biology ,010606 plant biology & botany - Abstract
International audience; Medicago truncatula is widely used as a model legume for symbiotic and pathogenic microbial interaction studies. Although a number of Agrobacterium-mediated transformation methods have been developed for M. truncatula, a rapid root transformation system was not yet available for this model plant. Here, we describe an easy method for rapid transgene expression in root hairs of M. truncatula, using young seedlings co-cultivated with the disarmed hypervirulent A. tumefaciens strain AGL1. This method leads to efficient expression of various GUS and fluorescent reporters in M. truncatula root hairs. We showed that transgene expression is detected as soon as 2 days following co-culture, in root hairs of a particular responsive zone lying 0.5–2 cm behind the root tip. This method can be used with a variety of M. truncatula genotypes, and is particularly useful for rapid investigation of the sub-cellular localization of fluorescent fusion proteins. Moreover, combining distinct Agrobacterium strains during the initial co-culture step efficiently generates co-transformed root hairs, suitable for co-localization of different fluorescent fusion proteins in the same cell.
- Published
- 2017
8. The <scp>ERN</scp> 1 transcription factor gene is a target of the <scp>CC</scp> a <scp>MK</scp> / <scp>CYCLOPS</scp> complex and controls rhizobial infection in Lotus japonicus
- Author
-
Jessica Folgmann, Lisa Frances, Andreas Klingl, Trevor L. Wang, Martin Parniske, Paul Stolz, Fernanda de Carvalho-Niebel, J. Allan Downie, Fang Xie, Xiaolin Li, Quanhui Wang, Katja Katzer, Anne Birgitte Lau Heckmann, and Marion R. Cerri
- Subjects
0106 biological sciences ,0301 basic medicine ,education.field_of_study ,Physiology ,fungi ,Mutant ,Lotus japonicus ,Population ,food and beverages ,Plant Science ,Biology ,biology.organism_classification ,01 natural sciences ,Microbiology ,Cell biology ,03 medical and health sciences ,Transactivation ,030104 developmental biology ,Transcription (biology) ,Transcription Factor Gene ,education ,Transcription factor ,Gene ,010606 plant biology & botany - Abstract
Bacterial accommodation inside living plant cells is restricted to the nitrogen-fixing root nodule symbiosis. In many legumes, bacterial uptake is mediated via tubular structures called infection threads (ITs). To identify plant genes required for successful symbiotic infection, we screened an ethyl methanesulfonate mutagenized population of Lotus japonicus for mutants defective in IT formation and cloned the responsible gene, ERN1, encoding an AP2/ERF transcription factor. We performed phenotypic analysis of two independent L. japonicus mutant alleles and investigated the regulation of ERN1 via transactivation and DNA-protein interaction assays. In ern1 mutant roots, nodule primordia formed, but most remained uninfected and bacterial entry via ITs into the root epidermis was abolished. Infected cortical nodule cells contained bacteroids, but transcellular ITs were rarely observed. A subset exhibited localized cell wall degradation and loss of cell integrity associated with bacteroid spread into neighbouring cells and the apoplast. Functional promoter studies revealed that CYCLOPS binds in a sequence-specific manner to a motif within the ERN1 promoter and in combination with CCaMK positively regulates ERN1 transcription. We conclude that the activation of ERN1 by CCaMK/CYCLOPS complex is an important step controlling IT-mediated bacterial progression into plant cells.
- Published
- 2017
9. NIN Acts as a Network Hub Controlling a Growth Module Required for Rhizobial Infection
- Author
-
Guru V. Radhakrishnan, Kirsty Jackson, Caitlin Bone, Fernanda de Carvalho-Niebel, Fran Robson, Suyu Jiang, Nicola Stacey, Giles E. D. Oldroyd, Jeremy D. Murray, Cheng-Wu Liu, Martin Trick, Dian Guan, Marion R. Cerri, Sonali Roy, Andreas Niebel, Christian Rogers, Andrew Breakspear, Department of Cell Biology, John Innes Centre, Norwich Research Park, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Chinese Academy of Sciences [Beijing] (CAS)-Shanghai Institutes for Biological Sciences-Institute of Plant Physiology and Ecology, Department of Cell and Developmental Biology, University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC)-University of North Carolina System (UNC), John Innes Centre [Norwich], Sainsbury Laboratory Cambridge, University of Cambridge [UK] (CAM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Chinese Academy of Sciences [Changchun Branch] (CAS), Biotechnology and Biological Sciences Research Council (BBSRC) : BB/L010305/1, John Innes Foundation, ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-15-CE20-0012,NODCCAAT,Comprendre le mode d'action du facteur de transcription NF-YA1 spécifique de l'intearction symbiotique rhizobium-légumineuses chez Medicago truncatula(2015), and ANR-14-CE35-0007,COME-IN,Cell-specific reprogramming of legume roots for endosymbiotic infection(2014)
- Subjects
0106 biological sciences ,Physiology ,[SDV]Life Sciences [q-bio] ,Plant Science ,Cyclopentanes ,Root hair ,01 natural sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Medicago truncatula ,Genetics ,Gene Regulatory Networks ,Oxylipins ,Gene ,Transcription factor ,ComputingMilieux_MISCELLANEOUS ,Plant Proteins ,Regulation of gene expression ,biology ,Jasmonic acid ,Articles ,biology.organism_classification ,Gibberellins ,Cell biology ,Biosynthetic Pathways ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Regulon ,chemistry ,Pectate lyase ,Rhizobium ,010606 plant biology & botany - Abstract
International audience; The symbiotic infection of root cells by nitrogen-fixing rhizobia during nodulation requires the transcription factor Nodule Inception (NIN). Our root hair transcriptomic study extends NIN's regulon to include Rhizobium Polar Growth and genes involved in cell wall modification, gibberellin biosynthesis, and a comprehensive group of nutrient (N, P, and S) uptake and assimilation genes, suggesting that NIN's recruitment to nodulation was based on its role as a growth module, a role shared with other NIN-Like Proteins. The expression of jasmonic acid genes in nin suggests the involvement of NIN in the resolution of growth versus defense outcomes. We find that the regulation of the growth module component Nodulation Pectate Lyase by NIN, and its function in rhizobial infection, are conserved in hologalegina legumes, highlighting its recruitment as a major event in the evolution of nodulation. We find that Nodulation Pectate Lyase is secreted to the infection chamber and the lumen of the infection thread. Gene network analysis using the transcription factor mutants for ERF Required for Nodulationl and Nuclear Factor-Y Subunit Al confirms hierarchical control of NIN over Nuclear Factor-Y Subunit Al and shows that ERF Required for Nodulationl acts independently to control infection. We conclude that while NIN shares functions with other NIN-Like Proteins, the conscription of key infection genes to NIN's control has made it a central regulatory hub for rhizobial infection.
- Published
- 2019
10. PUB1 Interacts with the Receptor Kinase DMI2 and Negatively Regulates Rhizobial and Arbuscular Mycorrhizal Symbioses through Its Ubiquitination Activity in Medicago truncatula
- Author
-
Richard D. Thompson, Ton Timmers, Tatiana Vernié, Céline Camps, Sylvie Camut, Malick Mbengue, Christine Hervé, Fernanda de Carvalho-Niebel, Julie V. Cullimore, Virginie Gasciolli, Christine Le Signor, Benoit Lefebvre, Céline Remblière, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agence Nationale Recherche [ANR-12-BSV7-0001, ANR-09-BLAN-0241-01], Grain Legumes Integrated Project of the European Commission FP6 Framework Program [FOOD-CT-2004-506223], TILLING program, ANR-12-BSV7-0001,SYMNALING,Signalisation symbiotique : mécanismes d' activation et spécificité dans la transduction des signaux Myc et Nod(2012), ANR-09-BLAN-0241,MycSignalling(2009), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), and European Project: ,FP6 Framework Program_FOOD-CT-2004-506223
- Subjects
0106 biological sciences ,0301 basic medicine ,Rhizophagus irregularis ,lotus-japonicus ,Root nodule ,Physiology ,[SDV]Life Sciences [q-bio] ,Colony Count, Microbial ,plant ,Plant Science ,01 natural sciences ,Glomeromycota ,Mycorrhizae ,Phosphorylation ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,Plant Proteins ,biology ,Kinase ,plasma-membrane ,Articles ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Medicago truncatula ,Ubiquitin ligase ,Cell biology ,Signal transduction ,Rhizobium ,Ubiquitin-Protein Ligases ,Saccharomyces cerevisiae ,03 medical and health sciences ,nod factor ,Protein Domains ,Symbiosis ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Botany ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,pathway ,fungi ,Ubiquitination ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,biology.organism_classification ,infection ,root-nodules ,arabidopsis ,030104 developmental biology ,biology.protein ,protein ,010606 plant biology & botany - Abstract
International audience; PUB1, an E3 ubiquitin ligase, which interacts with and is phosphorylated by the LYK3 symbiotic receptor kinase, negatively regulates rhizobial infection and nodulation during the nitrogen-fixing root nodule symbiosis in Medicago truncatula. In this study, we show that PUB1 also interacts with and is phosphorylated by DOES NOT MAKE INFECTIONS 2, the key symbiotic receptor kinase of the common symbiosis signaling pathway, required for both the rhizobial and the arbuscular mycorrhizal (AM) endosymbioses. We also show here that PUB1 expression is activated during successive stages of root colonization by Rhizophagus irregularis that is compatible with its interaction with DOES NOT MAKE INFECTIONS 2. Through characterization of a mutant, pub1-1, affected by the E3 ubiquitin ligase activity of PUB1, we have shown that the ubiquitination activity of PUB1 is required to negatively modulate successive stages of infection and development of rhizobial and AM symbioses. In conclusion, PUB1 represents, to our knowledge, a novel common component of symbiotic signaling integrating signal perception through interaction with and phosphorylation by two key symbiotic receptor kinases, and downstream signaling via its ubiquitination activity to fine-tune both rhizobial and AM root endosymbioses.
- Published
- 2016
11. The NIN Transcription Factor Coordinates Diverse Nodulation Programs in Different Tissues of the Medicago truncatula Root
- Author
-
Tatiana Vernié, Lisa Frances, Giles E. D. Oldroyd, Miriam L. Gifford, Andreas Niebel, Fernanda de Carvalho-Niebel, Jongho Sun, Dian Guan, Jiyoung Kim, Yiliang Ding, John Innes Centre [Norwich], Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), University of Warwick [Coventry], Biotechnology and Biological Sciences Research Council (BBSRC) : BB/J001872/1, Biotechnology and Biological Sciences Research Council (BBSRC) : BB/K003712/1, Biotechnology and Biological Sciences Research Council (BBSRC) : BB/J001503/1, Biotechnology and Biological Sciences Research Council (BBSRC) : BBS/E/J/000CA413, European Project: 255467,EC:FP7:PEOPLE,FP7-PEOPLE-2009-IEF,GRAS NOD SIGNALLING(2010), Biotechnology and Biological Sciences Research Council (BBSRC), University of California [San Diego] (UC San Diego), University of California, and Norwegian University of Life Sciences (NMBU)
- Subjects
Root nodule ,Cytokinins ,[SDV]Life Sciences [q-bio] ,Organogenesis ,Plant Science ,Plant Root Nodulation ,Plant Roots ,Nod factor ,chemistry.chemical_compound ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Genes, Reporter ,Nitrogen Fixation ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Botany ,Medicago truncatula ,Tobacco ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Symbiosis ,Research Articles ,Plant Proteins ,biology ,Epidermis (botany) ,fungi ,food and beverages ,Cell Biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Plants, Genetically Modified ,Cortex (botany) ,Cell biology ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,chemistry ,Cytokinin ,Calcium ,Root Nodules, Plant ,human activities ,Signal Transduction ,Sinorhizobium meliloti ,Transcription Factors - Abstract
International audience; Biological nitrogen fixation in legumes occurs in nodules that are initiated in the root cortex following Nod factor recognition at the root surface, and this requires coordination of diverse developmental programs in these different tissues. We show that while early Nod factor signaling associated with calcium oscillations is limited to the root surface, the resultant activation of Nodule Inception (NIN) in the root epidermis is sufficient to promote cytokinin signaling and nodule organogenesis in the inner root cortex. NIN or a product of its action must be associated with the transmission of a signal between the root surface and the cortical cells where nodule organogenesis is initiated. NIN appears to have distinct functions in the root epidermis and the root cortex. In the epidermis, NIN restricts the extent of Early Nodulin 11 (ENOD11) expression and does so through competitive inhibition of ERF Required for Nodulation (ERN1). In contrast, NIN is sufficient to promote the expression of the cytokinin receptor Cytokinin Response 1 (CRE1), which is restricted to the root cortex. Our work in Medicago truncatula highlights the complexity of NIN action and places NIN as a central player in the coordination of the symbiotic developmental programs occurring in differing tissues of the root that combined are necessary for a nitrogen-fixing symbiosis.
- Published
- 2016
12. The Symbiosis-Related ERN Transcription Factors Act in Concert to Coordinate Rhizobial Host Root Infection
- Author
-
Audrey Kelner, Monique Erard, Marion R. Cerri, Joëlle Fournier, Jiangqi Wen, Patrick H. Middleton, David G. Barker, Kirankumar S. Mysore, Fernanda de Carvalho-Niebel, Giles E. D. Oldroyd, Marie-Christine Auriac, Lisa Frances, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), University of California [San Diego] (UC San Diego), University of California, The Samuel Roberts Noble Foundation, Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), ANR-14-CE35-0007-01 COME-IN, LABEX TULIP (grant nos. ANR-10-LABX-41 and ANR-11-IDEX-0002-02), Ludwig Maximilian University [Munich] (LMU), John Innes Centre [Norwich], Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, French Ministry of Education, National Science Foundation (NSF)/NSF - Directorate for Biological Sciences (BIO) : 1127155, ANR-14-CE35-0007,COME-IN,Cell-specific reprogramming of legume roots for endosymbiotic infection(2014), and ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
- Subjects
0106 biological sciences ,0301 basic medicine ,Root nodule ,Transcription, Genetic ,Physiology ,Organogenesis ,[SDV]Life Sciences [q-bio] ,Mutant ,Plant Science ,Plant Roots ,01 natural sciences ,Plant Epidermis ,Gene Expression Regulation, Plant ,Mycorrhizae ,Promoter Regions, Genetic ,ComputingMilieux_MISCELLANEOUS ,Plant Proteins ,integumentary system ,biology ,Articles ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Phenotype ,Medicago truncatula ,Cell biology ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,[SDE]Environmental Sciences ,Rhizobium ,Root Nodules, Plant ,Protein Binding ,Signal Transduction ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Rhizobia ,03 medical and health sciences ,Symbiosis ,Nitrogen Fixation ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Botany ,otorhinolaryngologic diseases ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Amino Acid Sequence ,Transcription factor ,Alleles ,Plant Diseases ,Base Sequence ,biology.organism_classification ,030104 developmental biology ,Mutation ,sense organs ,Transcription Factors ,010606 plant biology & botany - Abstract
International audience; Legumes improve their mineral nutrition through nitrogen-fixing root nodule symbioses with soil rhizobia. Rhizobial infection of legumes is regulated by a number of transcription factors, including ERF Required for Nodulation1 (ERN1). Medicago truncatula plants defective in ERN1 are unable to nodulate, but still exhibit early symbiotic responses including rhizobial infection. ERN1 has a close homolog, ERN2, which shows partially overlapping expression patterns. Here we show that ern2 mutants exhibit a later nodulation phenotype than ern1, being able to form nodules but with signs of premature senescence. Molecular characterization of the ern2-1 mutation reveals a key role for a conserved threonine for both DNA binding and transcriptional activity. In contrast to either single mutant, the double ern1-1 ern2-1 line is completely unable to initiate infection or nodule development. The strong ern1-1 ern2-1 phenotype demonstrates functional redundancy between these two transcriptional regulators and reveals the essential role of ERN1/ERN2 to coordinately induce rhizobial infection and nodule organogenesis. While ERN1/ERN2 act in concert in the root epidermis, only ERN1 can efficiently allow the development of mature nodules in the cortex, probably through an independent pathway. Together, these findings reveal the key roles that ERN1/ERN2 play at the very earliest stages of root nodule development.
- Published
- 2016
13. TheMedicago truncatulaE3 Ubiquitin Ligase PUB1 Interacts with the LYK3 Symbiotic Receptor and Negatively Regulates Infection and Nodulation
- Author
-
Susana Rivas, Benoit Lefebvre, Ton Timmers, Dörte Klaus-Heisen, Laurent Deslandes, Christine Hervé, Malick Mbengue, Fernanda de Carvalho-Niebel, Julie V. Cullimore, Solène Froidure, Sylvie Camut, Sandra Moreau, Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), European Community funded Research Training Network 'NODPERCEPTION', French Ministry for Higher Education and Research, and ANR-05-BLAN-0243,NodBindsLysM,Etude multidisciplinaire des Interactions signal Nod / LysM récepteurs kinases dans la symbiose Rhizobia-Légumineuses(2005)
- Subjects
0106 biological sciences ,symbiotic receptor ,Plant Science ,luzerne ,Plant Root Nodulation ,01 natural sciences ,nitrogen ,Nod factor ,Ubiquitin ,Gene Expression Regulation, Plant ,medicago trunculata ,Génétique des plantes ,sinorhizobium meliloti ,nodulation ,Research Articles ,Plant Proteins ,azote ,0303 health sciences ,Sinorhizobium meliloti ,fourrage ,biology ,Kinase ,ubiquitin ligase ,fabaceae ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Plants, Genetically Modified ,Medicago truncatula ,Ubiquitin ligase ,Cell biology ,Biochemistry ,RNA Interference ,Signal transduction ,symbiose ,récepteur ,Signal Transduction ,Ubiquitin-Protein Ligases ,Molecular Sequence Data ,Plants genetics ,légumineuse ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Two-Hybrid System Techniques ,Tobacco ,Symbiosis ,030304 developmental biology ,fungi ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Biology ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,infection ,[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding ,Protein kinase domain ,biology.protein ,bacteria ,010606 plant biology & botany - Abstract
L'article original est publié par The American Society of Plant Biologists; International audience; LYK3 is a lysin motif receptor-like kinase of Medicago truncatula, which is essential for the establishment of the nitrogenfixing, root nodule symbiosis with Sinorhizobium meliloti. LYK3 is a putative receptor of S. meliloti Nod factor signals, but little is known of how it is regulated and how it transduces these symbiotic signals. In a screen for LYK3-interacting proteins, we identified M. truncatula Plant U-box protein 1 (PUB1) as an interactor of the kinase domain. In planta, both proteins are localized and interact in the plasma membrane. In M. truncatula, PUB1 is expressed specifically in symbiotic conditions, is induced by Nod factors, and shows an overlapping expression pattern with LYK3 during nodulation. Biochemical studies show that PUB1 has a U-box–dependent E3 ubiquitin ligase activity and is phosphorylated by the LYK3 kinase domain. Overexpression and RNA interference studies in M. truncatula show that PUB1 is a negative regulator of the LYK3 signaling pathway leading to infection and nodulation and is important for the discrimination of rhizobia strains producing variant Nod factors. The potential role of PUB E3 ubiquitin ligases in controlling plant–microbe interactions and development through interacting with receptor-like kinases is discussed.
- Published
- 2010
14. A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
- Author
-
Martin Crespi, Pascal Gamas, Lisa Frances, Maël Baudin, Fernanda de Carvalho-Niebel, Carolina Rípodas, Flavio Antonio Blanco, Tom Laloum, Agnes Lepage, Federico Ariel, María Eugenia Zanetti, Andreas Niebel, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Universidad Nacional de Mar del Plata [Mar del Plata] (UNMdP), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French Ministry of Education and Research, Institut National de la Recherche Agronomique Contrat Jeune Scientifique, Centre National de la Recherche Scientifique-Consejo Nacional de Investigaciones Cientificas y Tecnicas exchange program Projet International de Cooperation Scientifique : PICS06688, ANR-09-BLAN-0033,HAPIHUB(2009), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), and Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
0106 biological sciences ,Physiology ,Otras Ciencias Biológicas ,CAAT box ,Plant Science ,01 natural sciences ,Rhizobia ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,03 medical and health sciences ,Bimolecular fluorescence complementation ,Heterotrimeric G protein ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Genetics ,Transcription factors ,Dna binding ,nodulation ,purl.org/becyt/ford/1.6 [https] ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,Sinorhizobium meliloti ,biology ,C SUBUNIT ,Plant Root Nodulation ,fungi ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,medicago ,Medicago truncatula ,symbiosis ,Srtess-response ,Gras family ,Rhizobium ,Factor NF-Y ,Root nodule development ,CIENCIAS NATURALES Y EXACTAS ,010606 plant biology & botany - Abstract
The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation of symbiotic genes that are controlled by a set of early transcription factors (TFs). MtNF-YA1 and MtNF-YA2 are two TFs playing partially redundant functions during several steps of the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti. NF-Y proteins are part of a transcriptional complex composed of three proteins (NF-YA, NF-YB and NF-YC) which bind DNA at CCAAT-boxes, a motif present in most eukaryotic promoters. In plants, each subunit is encoded by small gene families, potentially leading to a multitude of heterotrimeric NF-Y complexes. Here, using yeast two hybrid screenings, we identified the MtNF-YB and MtNF-YC subunits that interact with MtNF-YA1 and A2. Further, we confirmed, both in yeast and in planta, the formation of trimeric NF-Y complexes and showed that these complexes are functional during nodulation using reverse genetic approaches and ChIP-PCR. Finally, as orthologs of the characterized NF-Y subunits also control nodulation in other legumes, we showed in common bean that similar NF-Y trimers could form in planta. Our results suggest that we have identified a group of evolutionary conserved NF-Y proteins that interact to control nodulation in leguminous plants. Fil: Baudin, Maël. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia Fil: Laloum, Tom. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia Fil: Lepage, Agnes. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia Fil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre D'etudes de Saclay; Francia Fil: Frances, Lisa. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia Fil: Crespi, Martin. Centre D'etudes de Saclay; Francia Fil: Gamas, Pascal. Centre National de la Recherche Scientifique; Francia Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: de Carvalho-Niebel, Fernanda. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia Fil: Niebel, Andreas. Centre National de la Recherche Scientifique; Francia. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
- Published
- 2015
15. Remodeling of the infection chamber before infection thread formation reveals a two-step mechanism for rhizobial entry into the host legume root hair
- Author
-
David G. Barker, Erik Limpens, Joëlle Fournier, Mireille Chabaud, Sergey Ivanov, Andrea Genre, Alice Teillet, Fernanda de Carvalho-Niebel, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research Centre (WUR), Universita di Torino, French National Research Agency [ANR-08-BLAN-0029-01], Partenariat Hubert Curien Galilee [30111WJ], French National Laboratoire d'Excellence TULIP initiative [ANR-10-LABX-41], Wageningen University and Research [Wageningen] (WUR), University of Turin, The Partenariat Hubert Curien Galilee (grant no. 30111WJ to A.G. and D.G.B.), ANR-08-BLAN-0029,SYMDYNAMICS,Mécanismes d'accommodation endosymbiotique chez les végétaux: Dynamique intracellulaire et signalisation calcique(2008), and ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
- Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,Physiology ,[SDV]Life Sciences [q-bio] ,Mutant ,Plant Science ,Root hair ,Exocytosis ,Rhizobia ,Cell wall ,Symbiosis ,Botany ,Genetics ,Laboratorium voor Moleculaire Biologie ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Life Science ,Sinorhizobium meliloti ,EPS-1 ,biology ,fungi ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Medicago truncatula ,Cell biology ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Laboratory of Molecular Biology - Abstract
International audience; In many legumes, root entry of symbiotic nitrogen-fixing rhizobia occurs via host-constructed tubular tip-growing structures known as infection threads (ITs). Here, we have used a confocal microscopy live-tissue imaging approach to investigate early stages of IT formation in Medicago truncatula root hairs (RHs) expressing fluorescent protein fusion reporters. This has revealed that ITs only initiate 10 to 20 h after the completion of RH curling, by which time major modifications have occurred within the so-called infection chamber, the site of bacterial entrapment. These include the accumulation of exocytosis (M. truncatula Vesicle-Associated Membrane Protein721e)- and cell wall (M. truncatula EARLY NODULIN11)-associated markers, concomitant with radial expansion of the chamber. Significantly, the infection-defective M. truncatula nodule inception-1 mutant is unable to create a functional infection chamber. This underlines the importance of the NIN-dependent phase of host cell wall remodeling that accompanies bacterial proliferation and precedes IT formation, and leads us to propose a two-step model for rhizobial infection initiation in legume RHs.
- Published
- 2015
16. The Medicago truncatula SUNN Gene Encodes a CLV1-like Leucine-rich Repeat Receptor Kinase that Regulates Nodule Number and Root Length
- Author
-
Fernanda de Carvalho-Niebel, Elise Schnabel, Gérard Duc, Etienne-Pascal Journet, Julia Frugoli, Clemson University, Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, UMR 0102 - Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, USDA Grant (2002-35319-12529), CREES SC-1700150 to JF, ACI Ministère de la Recherche (2001-03) (02N60/0546), INRA-SPE Grant (0441-01) (2002-03) to EPJ, and Technical Contribution No. 5054 of the Clemson University Experiment Station
- Subjects
0106 biological sciences ,Mutant ,Lotus japonicus ,Plant Science ,Leucine-rich repeat ,Biology ,Genes, Plant ,Plant Roots ,Synteny ,01 natural sciences ,Gene Expression Regulation, Enzymologic ,03 medical and health sciences ,SUNN ,Gene Expression Regulation, Plant ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Arabidopsis ,Genetics ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,GENETIQUE VEGETALE ,RLP1 ,Gene ,Phylogeny ,030304 developmental biology ,Regulation of gene expression ,0303 health sciences ,Nitrates ,fungi ,Receptor Protein-Tyrosine Kinases ,General Medicine ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Medicago truncatula ,NODULE REGULATION ,Phenotype ,SUPERNODULATION ,Mutation ,MEDICAGO TRUNCATULA ,Agronomy and Crop Science ,Plant Shoots ,010606 plant biology & botany - Abstract
International audience; Four Medicago truncatula sunn mutants displayed shortened roots and hypernodulation under all conditions examined. The mutants, recovered in three independent genetic screens, all contained lesions in a leucine-rich repeat (LRR) receptor kinase. Although the molecular defects among alleles varied, root length and the extent of nodulation were not significantly different between the mutants. SUNN is expressed in shoots, flowers and roots. Although previously reported grafting experiments showed that the presence of the mutated SUNN gene in roots does not confer an obvious phenotype, expression levels of SUNN mRNA were reduced in sunn-1 roots. SUNN and the previously identified genes HAR1 (Lotus japonicus) and NARK (Glycine max) are orthologs based on gene sequence and synteny between flanking sequences. Comparison of related LRR receptor kinases determined that all nodulation autoregulation genes identified to date are the closest legume relatives of AtCLV1 by sequence, yet sunn, har and nark mutants do not display the fasciated clv phenotype. The M. truncatula region is syntenic with duplicated regions of Arabidopsis chromosomes 2 and 4, none of which harbor CLV1 or any other LRR receptor kinase genes. A novel truncated copy of the SUNN gene lacking a kinase domain, RLP1, is found immediately upstream of SUNN and like SUNN is expressed at a reduced level in sunn-1 roots.
- Published
- 2005
17. Two CCAAT-box-binding transcription factors redundantly regulate early steps of the legume-rhizobia endosymbiosis
- Author
-
Benjamin Billault-Penneteau, Andreas Niebel, Marion R. Cerri, Tom Laloum, Maël Baudin, Pascal Gamas, Agnes Lepage, Lisa Frances, Federico Ariel, Marie-Françoise Jardinaud, Fernanda de Carvalho-Niebel, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences du végétal (ISV), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), École nationale supérieure agronomique de Toulouse [ENSAT], French Ministry of Education and Research, INRA CJS (Contrat Jeune Scientifique) contract, European Molecular Biology Organization (EMBO), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-09-BLAN-0033,HAPIHUB(2009), ANR-08-GENM-0015,SYMbiMICS,Dissection moléculaire de l'interaction symbiotique rhizobium-légumineuse : une approche combinée de micro-dissection laser et de séquençage massif d?ESTs(2008), Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Faculty of Biology, Adam Mickiewicz University in Poznań (UAM), Ludwig Maximilians University of Munich, UPR2355 Institut des sciences du végétal, and Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV]Life Sciences [q-bio] ,CAAT box ,Gene Expression ,Plant Science ,Plant Roots ,Rhizobia ,Nod factor ,Transactivation ,Gene Expression Regulation, Plant ,Genes, Reporter ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Tobacco ,Botany ,Medicago truncatula ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,nuclear factor Y ,Symbiosis ,Transcription factor ,Gene ,transcription factor ,Plant Proteins ,Nod factor signaling ,biology ,Endosymbiosis ,Sequence Analysis, RNA ,Cell Biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Cell biology ,legume-rhizobium symbiosis ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,CCAAT-Binding Factor ,RNA, Plant ,CCAAT box-binding factor ,Root Nodules, Plant ,Microdissection ,Signal Transduction ,Sinorhizobium meliloti ,Transcription Factors - Abstract
International audience; During endosymbiotic interactions between legume plants and nitrogen-fixing rhizobia, successful root infection by bacteria and nodule organogenesis requires the perception and transduction of bacterial lipo-chitooligosaccharidic signal called Nod factor (NF). NF perception in legume roots leads to the activation of an early signaling pathway and of a set of symbiotic genes which is controlled by specific early transcription factors (TFs) including CYCLOPS/IPD3, NSP1, NSP2, ERN1 and NIN. In this study, we bring convincing evidence that the Medicago truncatula CCAAT-box-binding NF-YA1 TF, previously associated with later stages of rhizobial infection and nodule meristem formation is, together with its closest homolog NF-YA2, also an essential positive regulator of the NF-signaling pathway. Here we show that NF-YA1 and NF-YA2 are both expressed in epidermal cells responding to NFs and their knock-down by reverse genetic approaches severely affects the NF-induced expression of symbiotic genes and rhizobial infection. Further over-expression, transactivation and ChIP-PCR approaches indicate that NF-YA1 and NF-YA2 function, at least in part, via the direct activation of ERN1. We thus propose a model in which NF-YA1 and NF-YA2 appear as early symbiotic regulators acting downstream of DMI3 and NIN and possibly within the same regulatory complexes as NSP1/2 to directly activate the expression of ERN1.
- Published
- 2014
18. An integrated analysis of plant and bacterial gene expression in symbiotic root nodules using laser-capture microdissection coupled to RNA sequencing
- Author
-
Ludovic Cottret, Sébastien Carrère, Pascal Gamas, Fernanda de Carvalho-Niebel, Jérôme Gouzy, Laurent Sauviac, Sandra Moreau, Claude Bruand, Frédéric Debellé, Ton Timmers, Delphine Capela, Marie-Françoise Jardinaud, Erika Sallet, Nathalie Rodde, Brice Roux, Emmanuel Courcelle, Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, ANR [ANR-08-GENO-106], French Laboratory of Excellence project 'TULIP' [ANR-10-LABX-41, ANR-11-IDEX-0002-02], Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), École nationale supérieure agronomique de Toulouse [ENSAT], the FR AIB microscopy platform (Toulouse), ANR-08-GENM-0015,SYMbiMICS,Dissection moléculaire de l'interaction symbiotique rhizobium-légumineuse : une approche combinée de micro-dissection laser et de séquençage massif d?ESTs(2008), and ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
- Subjects
regulators ,Root nodule ,[SDV]Life Sciences [q-bio] ,Meristem ,Gene Expression ,Plant Science ,Computational biology ,Bacterial genome size ,Laser Capture Microdissection ,Plant Roots ,nitrogen-fixing symbiosis ,Gene Expression Regulation, Plant ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Nitrogen Fixation ,Gene expression ,Botany ,Medicago truncatula ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Symbiosis ,Gene ,Laser capture microdissection ,2. Zero hunger ,Sinorhizobium meliloti ,biology ,Sequence Analysis, RNA ,Gene Expression Profiling ,fungi ,food and beverages ,Cell Biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,15. Life on land ,biology.organism_classification ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Genes, Bacterial ,Root Nodules, Plant ,transcriptome ,model legume - Abstract
International audience; Rhizobium-induced root nodules are specialized organs for symbiotic nitrogen fixation. Indeterminate-type nodules are formed from an apical meristem and exhibit a spatial zonation which corresponds to successive developmental stages. To get a dynamic and integrated view of plant and bacterial gene expression associated with nodule development, we used a sensitive and comprehensive approach based upon oriented high-depth RNA sequencing coupled to laser microdissection of nodule regions. This study, focused on the association between the model legume Medicago truncatula and its symbiont Sinorhizobium meliloti, led to the production of 942million sequencing read pairs that were unambiguously mapped on plant and bacterial genomes. Bioinformatic and statistical analyses enabled in-depth comparison, at a whole-genome level, of gene expression in specific nodule zones. Previously characterized symbiotic genes displayed the expected spatial pattern of expression, thus validating the robustness of our approach. We illustrate the use of this resource by examining gene expression associated with three essential elements of nodule development, namely meristem activity, cell differentiation and selected signaling processes related to bacterial Nod factors and redox status. We found that transcription factor genes essential for the control of the root apical meristem were also expressed in the nodule meristem, while the plant mRNAs most enriched in nodules compared with roots were mostly associated with zones comprising both plant and bacterial partners. The data, accessible on a dedicated website, represent a rich resource for microbiologists and plant biologists to address a variety of questions of both fundamental and applied interest.
- Published
- 2013
19. Next-generation annotation of prokaryotic genomes with EuGene-P: application to Sinorhizobium meliloti 2011
- Author
-
Marie-Franc¸oise Jardinaud, Pascal Gamas, Claude Bruand, Sébastien Carrère, Brice Roux, Laurent Sauviac, Fernanda de Carvalho-Niebel, Thomas Faraut, Thomas Schiex, Delphine Capela, Jérôme Gouzy, Erika Sallet, Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), École nationale supérieure agronomique de Toulouse [ENSAT], Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), This work was supported by the Agence Nationale de la Recherche under grant ANR-08-GENO-106 'SYMbiMICS'., Schiex, Thomas, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Biométrie et Intelligence Artificielle (UBIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INPT, ANR-08-GENM-0015,SYMbiMICS,Dissection moléculaire de l'interaction symbiotique rhizobium-légumineuse : une approche combinée de micro-dissection laser et de séquençage massif d?ESTs(2008), and ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
- Subjects
RNA, Untranslated ,genome annotation ,[SDV]Life Sciences [q-bio] ,rhizobium ,prokaryote ,rna-seq ,Sigma Factor ,Biology ,Genome ,Regulon ,prokaryotes ,03 medical and health sciences ,Open Reading Frames ,Transcription (biology) ,Nitrogen Fixation ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[INFO]Computer Science [cs] ,[MATH]Mathematics [math] ,Molecular Biology ,Gene ,030304 developmental biology ,0303 health sciences ,Sinorhizobium meliloti ,030306 microbiology ,Chromosome Mapping ,Molecular Sequence Annotation ,General Medicine ,Genome project ,Full Papers ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Non-coding RNA ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Transcription Initiation Site ,Genome, Bacterial ,Software - Abstract
Chantier qualité GA; International audience; The availability of next-generation sequences of transcripts from prokaryotic organisms offers the opportunity to design a new generation of automated genome annotation tools not yet available for prokaryotes. In this work, we designed EuGene-P, the first integrative prokaryotic gene finder tool which combines a variety of high-throughput data, including oriented RNA-Seq data, directly into the prediction process. This enables the automated prediction of coding sequences (CDSs), untranslated regions, transcription start sites (TSSs) and non-coding RNA (ncRNA, sense and antisense) genes. EuGene-P was used to comprehensively and accurately annotate the genome of the nitrogen-fixing bacterium Sinorhizobium meliloti strain 2011, leading to the prediction of 6308 CDSs as well as 1876 ncRNAs. Among them, 1280 appeared as antisense to a CDS, which supports recent findings that antisense transcription activity is widespread in bacteria. Moreover, 4077 TSSs upstream of protein-coding or noncoding genes were precisely mapped providing valuable data for the study of promoter regions. By looking for RpoE2-binding sites upstream of annotated TSSs, we were able to extend the S. meliloti RpoE2 regulon by similar to 3-fold. Altogether, these observations demonstrate the power of EuGene-P to produce a reliable and high-resolution automatic annotation of prokaryotic genomes.
- Published
- 2013
20. Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection
- Author
-
Lisa Frances, David G. Barker, Tom Laloum, Marion R. Cerri, Fernanda de Carvalho-Niebel, Joëlle Fournier, Giles E. D. Oldroyd, Andreas Niebel, Marie-Christine Auriac, Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Dept Cell & Dev Biol, University College London (UCL), Laboratoire d'Excellence program TULIP [ANR-10-LABX-41], French Ministry of Education and Research, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), French Ministry of Education and Research (to M.R.C. and T.L.), Biotechnology and Biological Sciences Research Council (BBSRC) : BBS/E/J/00000603, and ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
- Subjects
0106 biological sciences ,Transcription, Genetic ,Physiology ,DNA-BINDING ,[SDV]Life Sciences [q-bio] ,Regulator ,Plant Science ,01 natural sciences ,Plant Root Nodulation ,Plant Epidermis ,03 medical and health sciences ,Transcription (biology) ,Gene Expression Regulation, Plant ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Medicago truncatula ,Genetics ,Transcriptional regulation ,LOTUS-JAPONICUS ,NODULATION FACTORS ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Plants Interacting with Other Organisms ,DEPENDENT PROTEIN-KINASE ,Promoter Regions, Genetic ,Symbiosis ,Transcription factor ,030304 developmental biology ,GENE-EXPRESSION ,Regulation of gene expression ,Cell Nucleus ,0303 health sciences ,biology ,ROOT HAIRS ,FACTOR SIGNAL-TRANSDUCTION ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,ERN1 ,CALCIUM SPIKING ,Mutation ,RECEPTOR KINASE ,Signal transduction ,Root Nodules, Plant ,010606 plant biology & botany ,SYMBIOTIC NODULE DEVELOPMENT ,Rhizobium ,Signal Transduction ,Transcription Factors - Abstract
Rhizobial nodulation factors (NFs) activate a specific signaling pathway in Medicago truncatula root hairs that involves the complex interplay of Nodulation Signaling Pathway1 (NSP1)/NSP2 GRAS and Ethylene Response Factor Required for Nodulation1 (ERN1) transcription factors (TFs) to achieve full ENOD11 transcription. ERN1 acts as a direct transcriptional regulator of ENOD11 through the activation of the NF-responsive “NF box.” Here, we show that NSP1, when combined with NSP2, can act as a strong positive regulator of ERN1 and ENOD11 transcription. Although ERN1 and NSP1/NSP2 both activate ENOD11, two separate promoter regions are involved that regulate expression during consecutive symbiotic stages. Our findings indicate that ERN1 is required to activate NF-elicited ENOD11 expression exclusively during early preinfection, while NSP1/NSP2 mediates ENOD11 expression during subsequent rhizobial infection. The relative contributions of ERN1 and the closely related ERN2 to the rhizobial symbiosis were then evaluated by comparing their regulation and in vivo dynamics. ERN1 and ERN2 exhibit expression profiles compatible with roles during NF signaling and subsequent infection. However, differences in expression levels and spatiotemporal profiles suggest specialized functions for these two TFs, ERN1 being involved in stages preceding and accompanying infection thread progression while ERN2 is only involved in certain stages of infection. By cross complementation, we show that ERN2, when expressed under the control of the ERN1 promoter, can restore both NF-elicited ENOD11 expression and nodule formation in an ern1 mutant background. This indicates that ERN1 and ERN2 possess similar biological activities and that functional diversification of these closely related TFs relies primarily on changes in tissue-specific expression patterns.
- Published
- 2012
21. AP2-ERF Transcription Factors Mediate Nod Factor–Dependent Mt ENOD11 Activation in Root Hairs via a Novel cis -Regulatory Motif
- Author
-
Alain Jauneau, David G. Barker, Laurent Sauviac, Fernanda de Carvalho-Niebel, Lisa Frances, Aurélien Boisson-Dernier, Andry Andriankaja, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Signalisation cellulaire et biotechnologie végétale (SCBV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-École nationale supérieure agronomique de Toulouse [ENSAT]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées
- Subjects
0106 biological sciences ,Transcriptional Activation ,Response element ,Molecular Sequence Data ,Repressor ,Plant Science ,Root hair ,Biology ,Regulatory Sequences, Nucleic Acid ,Genes, Plant ,01 natural sciences ,Plant Roots ,Plant Epidermis ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Two-Hybrid System Techniques ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Medicago truncatula ,Tobacco ,Amino Acid Sequence ,Promoter Regions, Genetic ,Gene ,Transcription factor ,Base Pairing ,Conserved Sequence ,Research Articles ,030304 developmental biology ,Plant Proteins ,Genetics ,Regulation of gene expression ,Cell Nucleus ,0303 health sciences ,Base Sequence ,Models, Genetic ,Activator (genetics) ,food and beverages ,Cell Biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Cell biology ,Up-Regulation ,Protein Transport ,Regulatory sequence ,010606 plant biology & botany ,Protein Binding ,Transcription Factors - Abstract
Rhizobium Nod factors (NFs) are specific lipochitooligosaccharides that activate host legume signaling pathways essential for initiating the nitrogen-fixing symbiotic association. This study describes the characterization of cis-regulatory elements and trans-interacting factors that regulate NF-dependent and epidermis-specific gene transcription in Medicago truncatula. Detailed analysis of the Mt ENOD11 promoter using deletion, mutation, and gain-of-function constructs has led to the identification of an NF-responsive regulatory unit (the NF box) sufficient to direct NF-elicited expression in root hairs. NF box–mediated expression requires a major GCC-like motif, which is also essential for the binding of root hair–specific nuclear factors. Yeast one-hybrid screening has identified three closely related AP2/ERF transcription factors (ERN1 to ERN3) that are able to bind specifically to the NF box. ERN1 is identical to an ERF-like factor identified recently. Expression analysis has revealed that ERN1 and ERN2 genes are upregulated in root hairs following NF treatment and that this activation requires a functional NFP gene. Transient expression assays in Nicotiana benthamiana have further shown that nucleus-targeted ERN1 and ERN2 factors activate NF box–containing reporters, whereas ERN3 represses ERN1/ERN2-dependent transcription activation. A model is proposed for the fine-tuning of NF-elicited gene transcription in root hairs involving the interplay between repressor and activator ERN factors.
- Published
- 2007
22. The Medicago truncatula Lysine Motif-Receptor-Like Kinase Gene Family Includes NFP and New Nodule-Expressed Genes
- Author
-
Anne Bersoult, Rossana Mirabella, Fernanda de Carvalho-Niebel, Clare Gough, Annick Barre, Thierry Huguet, Pierre Rougé, Michèle Ghérardi, Etienne-Pascal Journet, Lidia Campos Soriano, Besma Ben Amor, Jean-François Arrighi, Jean Dénarié, René Geurts, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR), and Plant Physiology (SILS, FNWI)
- Subjects
0106 biological sciences ,Subfamily ,Physiology ,Amino Acid Motifs ,protein-kinase ,Gene Expression ,Plant Science ,01 natural sciences ,Plant Roots ,Nod factor ,Arabidopsis ,Gene Duplication ,Phosphorylation ,Plant Proteins ,Genetics ,symbiotic genes ,0303 health sciences ,biology ,Autophosphorylation ,food and beverages ,legume ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Medicago truncatula ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Multigene Family ,RNA Interference ,Laboratory of Molecular Biology ,Genome, Plant ,Research Article ,Molecular Sequence Data ,rhizobium-meliloti ,root hairs ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,lysm domains ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Gene family ,Laboratorium voor Moleculaire Biologie ,Amino Acid Sequence ,Symbiosis ,Gene ,030304 developmental biology ,Lysine ,fungi ,biology.organism_classification ,infection ,Protein Structure, Tertiary ,arabidopsis ,Protein kinase domain ,genomic analysis ,Protein Kinases ,nodulation factor-perception ,010606 plant biology & botany ,Sinorhizobium meliloti - Abstract
Rhizobial Nod factors are key symbiotic signals responsible for starting the nodulation process in host legume plants. Of the six Medicago truncatula genes controlling a Nod factor signaling pathway, Nod Factor Perception (NFP) was reported as a candidate Nod factor receptor gene. Here, we provide further evidence for this by showing that NFP is a lysine motif (LysM)-receptor-like kinase (RLK). NFP was shown both to be expressed in association with infection thread development and to be involved in the infection process. Consistent with deviations from conserved kinase domain sequences, NFP did not show autophosphorylation activity, suggesting that NFP needs to associate with an active kinase or has unusual functional characteristics different from classical kinases. Identification of nine new M. truncatula LysM-RLK genes revealed a larger family than in the nonlegumes Arabidopsis (Arabidopsis thaliana) or rice (Oryza sativa) of at least 17 members that can be divided into three subfamilies. Three LysM domains could be structurally predicted for all M. truncatula LysM-RLK proteins, whereas one subfamily, which includes NFP, was characterized by deviations from conserved kinase sequences. Most of the newly identified genes were found to be expressed in roots and nodules, suggesting this class of receptors may be more extensively involved in nodulation than was previously known.
- Published
- 2006
23. MtENOD11 gene activation during rhizobial infection and mycorrhizal arbuscule development requires a common AT-rich-containing regulatory sequence
- Author
-
Aurélien Boisson-Dernier, Etienne P. Journet, Andreas Niebel, Mireille Chabaud, Fernanda de Carvalho-Niebel, David G. Barker, Andry Andriankaja, Laboratoire des interactions plantes micro-organismes (LIPM), and Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Transcriptional Activation ,Nematoda ,Transcription, Genetic ,Physiology ,Molecular Sequence Data ,meloidogyne ,Biology ,Regulatory Sequences, Nucleic Acid ,01 natural sciences ,Nod factor ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Mycorrhizae ,Gene expression ,Medicago truncatula ,Animals ,Promoter Regions, Genetic ,Symbiosis ,Gene ,030304 developmental biology ,Plant Proteins ,Genetics ,Regulation of gene expression ,0303 health sciences ,Sinorhizobium meliloti ,Reporter gene ,food and beverages ,AATAA motif ,General Medicine ,glomus ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Plants, Genetically Modified ,Agrobacterium rhizogenes ,nodFnodL ,AT Rich Sequence ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Regulatory sequence ,Agronomy and Crop Science ,Gene Deletion ,010606 plant biology & botany - Abstract
The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont. To identify the regulatory sequences involved in symbiosisrelated MtENOD11 expression, a series of promoter deletions driving the β-glucuronidase reporter gene were analyzed in transgenic M. truncatula roots. These studies have revealed that distinct regulatory regions are involved in infection-related MtENOD11 expression compared with preinfection (Nod factor-mediated) expression. In particular, the 257-bp promoter sequence immediately upstream from the start codon is sufficient for infection-related expression, but is unable to drive gene transcription in response to the Nod factor elicitor. This truncated promoter is also sufficient to confer MtENOD11 expression during both the arbuscular mycorrhizal (AM) association and the parasitic interaction with root-knot nematodes. Site-directed mutagenesis further showed that a previously identified nodule-specific AT-rich motif is required for high-level MtENOD11 expression during S. meliloti infection as well as during AM colonization. However, mutation of this motif does not affect gene expression associated with nematodefeeding sites. Taken together, these results suggest a close link between regulatory mechanisms controlling transcriptional early nodulin gene activation during both rhizobial and AM root endosymbioses. Additional keywords: AATAA motif, Agrobacterium rhizogenes, Glomus, Meloidogyne, nodFnodL.
- Published
- 2006
24. Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula
- Author
-
Aurélien Boisson-Dernier, Andreas Niebel, Fernanda de Carvalho-Niebel, Laurent Sauviac, David G. Barker, Laboratoire des interactions plantes micro-organismes (LIPM), and Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,legume symbiosis ,MtENOD11 ,Transcription, Genetic ,Physiology ,Plant Science ,Root hair ,01 natural sciences ,Plant Roots ,Polymerase Chain Reaction ,Nod factor ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Transcription (biology) ,Gene Expression Regulation, Plant ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Gene expression ,Botany ,Medicago truncatula ,real time quantitative RT-PCR ,030304 developmental biology ,DNA Primers ,Plant Proteins ,Regulation of gene expression ,0303 health sciences ,biology ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,MtENOD40 ,RNA ,Membrane Proteins ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Cell biology ,root hair-specific expression ,Rhizobium ,010606 plant biology & botany - Abstract
International audience; This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis. After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA. This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations. Furthermore, the use of these enriched RNA samples has revealed for the first time a very significant induction (30-fold) of the MtENOD40 gene in root hairs in response to Nod factors. It is concluded that the rapid and convenient procedure described here will be particularly useful for detecting tissue-specific low-level gene expression in root hairs responding to Rhizobium Nod factors or other exogenous signals.
- Published
- 2005
25. The Nod factor-elicited annexin MtAnn1 is preferentially localised at the nuclear periphery in symbiotically activated root tissues of Medicago truncatula
- Author
-
Mireille Chabaud, Fernanda de Carvalho-Niebel, Antonius C.J. Timmers, David G. Barker, Annie Defaux-Petras, Laboratoire des interactions plantes micro-organismes (LIPM), and Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Lipopolysaccharides ,Cellular differentiation ,Recombinant Fusion Proteins ,root nodulation ,Green Fluorescent Proteins ,Plant Science ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Biology ,01 natural sciences ,Plant Roots ,Nod factor ,03 medical and health sciences ,Annexin ,Gene Expression Regulation, Plant ,Botany ,Gene expression ,Genetics ,Medicago ,Fluorescent Antibody Technique, Indirect ,Symbiosis ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,030304 developmental biology ,Annexin A1 ,Regulation of gene expression ,Cell Nucleus ,0303 health sciences ,symbiotic signalling ,Lateral root ,fungi ,food and beverages ,Gene Expression Regulation, Developmental ,GFP/GUS-reporters ,Cell Differentiation ,Cell Biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,endodermis ,Plants, Genetically Modified ,Medicago truncatula ,Cell biology ,Luminescent Proteins ,Microscopy, Fluorescence ,Endodermis ,010606 plant biology & botany ,Signal Transduction ,Sinorhizobium meliloti - Abstract
International audience; The Medicago truncatula MtAnn1 gene, encoding a putative annexin, is transcriptionally activated in root tissues in response to rhizobial Nod factors. To gain further insight into MtAnn1 function during the early stages of nodulation, we have examined in detail both spatio-temporal gene expression patterns and MtAnn1 activity and localisation in root tissues. Analysis of transgenic Medicago plants expressing a pMtAnn1-GUS fusion has revealed a novel pattern of transcription in both outer and inner cell layers of the root following either Nod factor-treatment or rhizobial inoculation. The highest gene expression levels were observed in the endodermis and outer cortex. These transgenic plants also revealed that MtAnn1 expression is associated with lateral root development and cell differentiation in the root apex independent of nodulation. By purifying recombinant MtAnn1 we were able to demonstrate that this plant annexin indeed possesses the calcium-dependent binding to acidic phospholipids typical of the annexin family. Antisera against recombinant MtAnn1 were then used to show that tissue-specific localisation of the MtAnn1 protein in Medicago roots matches the pMtAnn1-GUS expression pattern. Finally, both immunolabelling and in vivo studies using MtAnn1-GFP reporter fusions have revealed that MtAnn1 is cytosolic and in particular localises to the nuclear periphery in cortical cells activated during the early stages of nodulation. In the light of our findings, we discuss the possible role of this annexin in root tissues responding to symbiotic rhizobial signals.
- Published
- 2002
26. Identification of New Medicago truncatula Nodulin Genes: Comparison of Two Molecular Approaches
- Author
-
Nicole Lescure, Pascal Gamas, Fernanda de Carvalho-Niebel, Julie V. Cullimore, Laboratoire des interactions plantes micro-organismes (LIPM), and Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,2. Zero hunger ,Genetics ,0303 health sciences ,biology ,fungi ,food and beverages ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,01 natural sciences ,Medicago truncatula ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Complementary DNA ,Identification (biology) ,Differential display technique ,Plant genes ,Gene ,030304 developmental biology ,010606 plant biology & botany - Abstract
International audience; This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab.Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.
- Published
- 1997
27. Differential in vitro DNA binding activity to a promoter element of the gn1 beta-1,3-glucanase gene in hypersensitively reacting tobacco plants
- Author
-
Elena Alonso, Patricia Obregón, Marc Van Montagu, Godelieve Gheysen, Carmen Castresana, Dirk Inzé, Fernanda de Carvalho Niebel, ProdInra, Migration, Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Universiteit Gent = Ghent University [Belgium] (UGENT), Hogeschool Gent, Unité associée au Département Biologie et Amélioration des Plantes (Associée BAP), and Institut National de la Recherche Agronomique (INRA)
- Subjects
0106 biological sciences ,DNA, Plant ,Molecular Sequence Data ,Plant Science ,Biology ,Genes, Plant ,01 natural sciences ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics ,Pseudomonas ,Gene expression ,Genes, Regulator ,Tobacco ,Genetics ,Promoter Regions, Genetic ,Gene ,030304 developmental biology ,Glucuronidase ,Regulation of gene expression ,0303 health sciences ,Reporter gene ,Binding Sites ,Base Sequence ,beta-Glucosidase ,Promoter ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Biology ,Glucan 1,3-beta-Glucosidase ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Plants, Genetically Modified ,Molecular biology ,Plants, Toxic ,chemistry ,Regulatory sequence ,Sequence motif ,DNA ,010606 plant biology & botany - Abstract
International audience; In a hypersensitive reaction to pathogen infection, expression of the beta-1,3-glucanase gn1 gene is induced in cells surrounding the necrotic lesions. The 5'-flanking sequence of gn1 was examined to investigate the molecular basis controlling activation of gene expression during this plant defense response. Studies on transgenic tobacco plants containing gn1 promoter deletions fused to the beta-glucuronidase reporter gene revealed the presence of negative and positive regulatory sequences mediating both the level and the spatial distribution of gn1 expression. Promoter sequences to -138 bp were sufficient to confer increased gene expression around the necrotic lesions produced in response to Pseudomonas syringae pv. syringae inoculation. It is demonstrated by electrophoretic mobility shift assays that nuclear proteins in both healthy and hypersensitively reacting tobacco leaves interact with DNA sequences within the regulatory elements identified. Among the binding sequences characterized, the promoter region extending from -250 to -217 bp contained the DNA motif -GGCGGC- found to be conserved in most if not all promoters of genes encoding pathogenesis-related basic proteins. The activity bound by this promoter sequence was stronger in hypersensitively responding tissues than in healthy untreated tobacco leaves.
- Published
- 1995
28. Transcription Reprogramming during Root Nodule Development in Medicago truncatula
- Author
-
Marion Verdenaud, Sébastien Letort, Pascal Gamas, Andreas Niebel, Françoise de Billy, Jérôme Gouzy, Sandra Moreau, Fernanda de Carvalho-Niebel, Thomas Ott, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Marie-Curie Intra-European Fellowship (024587-Nodule Remorin), ANR-06-GPLA-0005,LEGOO,LEGOO : a bioinformatics gateway towards integrative legume biology(2006), ANR-08-GENM-0015,SYMbiMICS,Dissection moléculaire de l'interaction symbiotique rhizobium-légumineuse : une approche combinée de micro-dissection laser et de séquençage massif d?ESTs(2008), European Project: FP6-GLIP, Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), EU INRA, Marie-Curie Intra-European Fellowship : 024587-Nodule Remorin, and Agence Nationale de la Recherche : ANR LEGoo GPLA06026G, ANR SYMbiMICS PCS-08-GENO-106
- Subjects
0106 biological sciences ,Root nodule ,Transcription, Genetic ,Plant Science ,modèle ,Plant Roots ,01 natural sciences ,Transcriptome ,Plant Microbiology ,Gene Expression Regulation, Plant ,Plant Genomics ,Jasmonate ,Regulator gene ,Plant Growth and Development ,Regulation of gene expression ,0303 health sciences ,Sinorhizobium meliloti ,Multidisciplinary ,Ecology ,biology ,food and beverages ,Cell Differentiation ,Genomics ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Medicago truncatula ,Functional Genomics ,Cell biology ,Community Ecology ,Medicine ,Root Nodules, Plant ,symbiose ,Research Article ,nodule ,Science ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,légumineuse ,03 medical and health sciences ,Model Organisms ,Nitrogen Fixation ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Botany ,Symbiosis ,Biology ,030304 developmental biology ,Gene Expression Profiling ,gène ,biology.organism_classification ,racine ,Gene expression profiling ,Species Interactions ,Genome Expression Analysis ,MEDICAGO TRUNCATULA ,Transcription Factors ,Developmental Biology ,010606 plant biology & botany - Abstract
International audience; Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are shared with other plant developmental programs also remains an open question. Here detailed microarray analyses have been used to study the transcriptome of root nodules induced by either wild type or mutant strains of Sinorhizobium meliloti. In this way we have defined eight major activation patterns in nodules and identified associated potential regulatory genes. We have shown that transcription reprogramming during consecutive stages of nodule differentiation occurs in four major phases, respectively associated with (i) early signalling events and/or bacterial infection; plant cell differentiation that is either (ii) independent or (iii) dependent on bacteroid differentiation; (iv) nitrogen fixation. Differential expression of several genes involved in cytokinin biosynthesis was observed in early symbiotic nodule zones, suggesting that cytokinin levels are actively controlled in this region. Taking advantage of databases recently developed for M. truncatula, we identified a small subset of gene expression regulators that were exclusively or predominantly expressed in nodules, whereas most other regulators were also activated under other conditions, and notably in response to abiotic or biotic stresses. We found evidence suggesting the activation of the jasmonate pathway in both wild type and mutant nodules, thus raising questions about the role of jasmonate during nodule development. Finally, quantitative RT-PCR was used to analyse the expression of a series of nodule regulator and marker genes at early symbiotic stages in roots and allowed us to distinguish several early stages of gene expression activation or repression.
- Published
- 2011
29. Post-Transcriptional Cosuppression of b-1,3-Glucanase Genes Does Not Affect Accumulation of Transgene Nuclear mRNA
- Author
-
Fernanda de Carvalho Niebel, Pierre Frendo, Marc Van Montagu, and Marc Cornelissen
- Subjects
Cell Biology ,Plant Science - Published
- 1995
30. Annexins - calcium- and membrane-binding proteins in the plant kingdom: potential role in nodulation and mycorrhization in Medicago truncatula
- Author
-
Karolina Maria Gorecka, Slawomir Pikula, Fernanda de Carvalho-Niebel, Tanuja Talukdar, Julie V. Cullimore, J. Allan Downie, Iwate Biotechnology Research Center (IBRC), Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), John Innes Centre [Norwich], NODPERCEPTION contract (MRTN-CT-2006-035546), Nencki Institute of Experimental Biology, Department of Biochemistry, Hôpital Lapeyronie, Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), BBSRC John Innes Centre, and Partenaires INRAE
- Subjects
0106 biological sciences ,Annexins ,[SDV]Life Sciences [q-bio] ,Molecular Sequence Data ,chemistry.chemical_element ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Calcium ,01 natural sciences ,DNA-binding protein ,Plant Root Nodulation ,General Biochemistry, Genetics and Molecular Biology ,Rhizobia ,Nod factor ,03 medical and health sciences ,Annexin ,Stress, Physiological ,Mycorrhizae ,Nod factors ,Medicago truncatula ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Amino Acid Sequence ,Symbiosis ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,030304 developmental biology ,Plant Proteins ,0303 health sciences ,Sinorhizobium meliloti ,biology ,Calcium-Binding Proteins ,fungi ,Membrane Proteins ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Biochemistry ,chemistry ,Signal transduction ,Sequence Alignment ,plant annexins ,010606 plant biology & botany ,Signal Transduction - Abstract
International audience; Annexins belong to a family of multi-functional membrane- and Ca2+-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca2+-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant counterparts were discovered only in 1989, in tomato, and have not been thoroughly studied yet. In the present review, we discuss the available information about plant annexins with special emphasis on biochemical and functional properties of some of them. In addition, we propose a link between annexins and symbiosis and Nod factor signal transduction in the legume plant, Medicago truncatula. A specific calcium response, calcium spiking, is an essential component of the Nod factor signal transduction pathway in legume plants. The potential role of annexins in the generation and propagation of this calcium signal is considered in this review. M. truncatula annexin 1 (MtAnn1) is a typical member of the plant annexin family, structurally similar to other members of the family. Expression of the MtAnn1 gene is specifically induced during symbiotic associations with both Sinorhizobium meliloti and the mycorrhizal fungus Glomus intraradices. Furthermore, it has been reported that the MtAnn1 protein is preferentially localized at the nuclear periphery of rhizobial-activated cortical cells, suggesting a possible role of this annexin in the calcium response signal elicited by symbiotic signals from rhizobia and mycorrhizal fungi.
31. The Medicago truncatula MtAnn1 Gene Encoding an Annexin Is Induced by Nod Factors and During the Symbiotic Interaction with Rhizobium meliloti
- Author
-
Fernanda de Carvalho Niebel, Nicole Lescure, Julie V. Cullimore, and Pascal Gamas
- Subjects
supernodulating mutant line ,symbiosis ,Microbiology ,QR1-502 ,Botany ,QK1-989 - Abstract
Here we report the characterization of a new Nod factor-induced gene from Medicago truncatula identified by mRNA differential display. This gene, designated MtAnn1, encodes a protein homologous to the annexin family of calcium- and phospholipid-binding proteins. We further show that the MtAnn1 gene is also induced during symbiotic associations with Rhizobium meliloti, both at early stages in bacterial-inoculated roots and in nodule structures. By in situ hybridization, we demonstrate that MtAnn1 expression in nodules is mainly associated with the distal region of invasion zone II not containing infection threads, revealing MtAnn1 as a new marker gene of the pre-infection zone. Moreover, analyses of MtAnn1 expression in response to bacterial symbiotic mutants suggest that the expression of MtAnn1 during nodulation requires biologically active Nod factors and is independent of the infection process.
- Published
- 1998
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.