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Two CCAAT-box-binding transcription factors redundantly regulate early steps of the legume-rhizobia endosymbiosis
- Source :
- Plant Journal, Plant Journal, Wiley, 2014, 79 (5), pp.757-768. ⟨10.1111/tpj.12587⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- International audience; During endosymbiotic interactions between legume plants and nitrogen-fixing rhizobia, successful root infection by bacteria and nodule organogenesis requires the perception and transduction of bacterial lipo-chitooligosaccharidic signal called Nod factor (NF). NF perception in legume roots leads to the activation of an early signaling pathway and of a set of symbiotic genes which is controlled by specific early transcription factors (TFs) including CYCLOPS/IPD3, NSP1, NSP2, ERN1 and NIN. In this study, we bring convincing evidence that the Medicago truncatula CCAAT-box-binding NF-YA1 TF, previously associated with later stages of rhizobial infection and nodule meristem formation is, together with its closest homolog NF-YA2, also an essential positive regulator of the NF-signaling pathway. Here we show that NF-YA1 and NF-YA2 are both expressed in epidermal cells responding to NFs and their knock-down by reverse genetic approaches severely affects the NF-induced expression of symbiotic genes and rhizobial infection. Further over-expression, transactivation and ChIP-PCR approaches indicate that NF-YA1 and NF-YA2 function, at least in part, via the direct activation of ERN1. We thus propose a model in which NF-YA1 and NF-YA2 appear as early symbiotic regulators acting downstream of DMI3 and NIN and possibly within the same regulatory complexes as NSP1/2 to directly activate the expression of ERN1.
- Subjects :
- [SDV]Life Sciences [q-bio]
CAAT box
Gene Expression
Plant Science
Plant Roots
Rhizobia
Nod factor
Transactivation
Gene Expression Regulation, Plant
Genes, Reporter
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Tobacco
Botany
Medicago truncatula
Genetics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
nuclear factor Y
Symbiosis
Transcription factor
Gene
transcription factor
Plant Proteins
Nod factor signaling
biology
Endosymbiosis
Sequence Analysis, RNA
Cell Biology
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
biology.organism_classification
Cell biology
legume-rhizobium symbiosis
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
CCAAT-Binding Factor
RNA, Plant
CCAAT box-binding factor
Root Nodules, Plant
Microdissection
Signal Transduction
Sinorhizobium meliloti
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 09607412 and 1365313X
- Database :
- OpenAIRE
- Journal :
- Plant Journal, Plant Journal, Wiley, 2014, 79 (5), pp.757-768. ⟨10.1111/tpj.12587⟩
- Accession number :
- edsair.doi.dedup.....b86fab6e60fa2f994b22b322ee65e693