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Made available in DSpace on 2020-12-12T02:20:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Breast cancer is one of the most lethal types of cancer and a leading cause of mortality among Women worldwide. Citrinin (CIT), a polyketide extracted from the fungus Penicillium citrinum, exhibits a wide range of biological activities such as antibacterial, antifungal, and cytotoxic effects. The aim of the current study was to evaluate the antitumoral effects of CIT against 7,12-dimethylbenzanthracene (DMBA)-induced mammary carcinoma in Swiss mice For this, CIT, DMBA and the standard cyclophosphamide (CPA) induced behavioral changes in experimental animals, and these changes were screened by using the rota rod and open field tests. Additionally, hematological, biochemical, immuno-histochemical, and histopathological analyses were carried out. Results suggest that CIT did not alter behavioral, hematological, and biochemical parameters in mice. DMBA induced invasive mammary carcinoma and showed genotoxic effects in the breasts, bone marrow, lymphocytes, and hepatic cells. It also caused mutagenic effects in the formation of micronuclei, bridges, shoots, and binucleate cells in bone marrow and liver. CIT and CPA genotoxic effects were observed after 3 weeks of therapy, where CIT exhibited a repair capacity and induced significant apoptotic damage in mouse lymphocytes. In conclusion, CIT showed antitumoral effects in Swiss mice, possibly through induction of apoptosis. Northeast Biotechnology Network (RENORBIO) Postgraduate Program in Biotechnology Federal University of Piauí – UFPI Laboratory of Research in Toxicological Genetics – LAPGENIC Federal University of Piauí Federal Institute of Piauí (IFPI) Nucleus of Applied Research to Sciences (NIAC) Federal Institute of Education Science and Technology of Maranhão (IFMA) Nucleus of Bioassays Biosynthesis and Ecophysiology of Natural Products (NuBBE) Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) Laboratory of Genetics and Molecular Biology Federal University of Maranhão Laboratory of Pharmaceutical Nanosystems – NANOSFAR Federal University of Piauí Laboratory of Theoretical and Computational Biophysics Ton Duc Thang University Faculty of Pharmacy Ton Duc Thang University Department of Chemistry The University of Jordan Nucleus of Bioassays Biosynthesis and Ecophysiology of Natural Products (NuBBE) Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)

Made available in DSpace on 2019-10-06T15:42:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 BDNF is associated with brain health and positively modulated by exercise; however, the influence of physical fitness status on BDNF is incipient. This study investigated the BDNF response after acute-exercise sessions performed at low, moderate, and high intensities and the relationship between physical fitness status and BDNF response. Twenty-eight men, divided according to physical fitness status (50th percentile for VO 2max ), performed three randomised acute exercise sessions at low (90% of VT1), moderate (midpoint between VT1-VT2), and high (midpoint between VT2-W max ) intensities until exhaustion or for up to 60 min. Lactate and BDNF were determined pre and post-exercises. For BDNF, there were main effects of time (p = 0.003) and interaction (p < 0.001), showing an increase post high-intensity exercise (p < 0.001). Changes in BDNF presented differences between conditions (p < 0.001) with greater increase in high-intensity compared with the others (p = 0.003). For lactate, there were main effects of time (p < 0.001), condition (p < 0.001), and interaction (p < 0.001) with greater concentration in high-intensity. High-intensity exercise exhibited inverse correlation between the changes in BDNF and lactate (r=−0.38, p = 0.044). There was significant correlation between BDNF and VO 2max for moderate (r = −0.57, p = 0.002) and a trend for high-intensity condition (r = −0.37, p = 0.050) and when evaluating BDNF according to physical fitness level, it was observed that subjects with lower physical fitness levels had greater increases in BDNF in short-time high-intensity exercise (p = 0.041). In conclusion, short-time high-intensity exercise seems to be more efficient in increasing BDNF concentration, and physical fitness level influences this response, as healthy individuals with lower physical fitness levels were more responsive. Exercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP) Immunometabolism of Skeletal Muscle and Exercise Research Group Department of Physical Education Federal University of Piauí Research center for Sport and Physical Activity (UID/PTD/04213/2016) Faculty of Sport Sciences and Physical Education University of Coimbra Associate Graduate Program in Health Science Federal University of Piauí (UFPI) Exercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP)

Made available in DSpace on 2022-04-28T17:22:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-09 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: Considering the importance of leptospirosis in sheep farming and public health and the significance of identifying which serogroups circulate in sheep within each region, the objective of this study was to determine the seroprevalence and spatial distribution of the most frequent serogroups causing infection by Leptospira sp. in ovine herds in the Northeast region of Brazil. Materials, Methods & Results: Blood samples were collected from 4197 sheep from 229 herds in 7 Northeastern States. Sera were analyzed via microscopic agglutination test (MAT). The frequency of seroreactive sheep for Leptospira sp. was 14.06%. The states of Alagoas, Ceara, Paraiba, Piaui, Rio Grande do Norte, and Sergipe, located in the Caatinga biome, had the highest frequencies of serologically reactive sheep, and Maranhao, in the Cerrado biome, had the lowest frequency. The most frequent serogroups were Autumnalis (19.49%), Australis (15.76%) and Serjoe (14.41%). In the states of Ceara, Paraiba, Rio Grande do Norte, and Sergipe, 100% of their municipalities had at least one seroreactive animal. The highest frequencies of seropositive animals were found in the municipalities of Uniao (50%), Passagem (49.06%), Caninde (48.89%), Igaci (28.95%), Gararu (31.2%), Pirapemas (17.5%), and Angicos (16%) located in the states of Piaui, Paraiba, Ceara, Alagoas, Sergipe, Maranhao and Rio Grande do Norte, respectively. Discussion: The animal-level prevalence (14.06%) obtained in the present study is significant, especially considering the rustic nature of the species and the adverse conditions of the region for the infectious agent. In semi-arid conditions, it has been suggested that perhaps sheep do not seroconvert detectable titers on MAT with a cut-off point of 1:100. It is important to highlight that the ovine population in the Northeast region of Brazil is composed of mixed animals, which have been considered more resistant to infection by Leptospira spp. Also, environmental factors hostile to the survival of the infectious agent in the studied region should be taken into consideration, since they may have influenced the seropositive animal-level prevalence. A noteworthy variation was observed in agglutinin titers, which ranged from 100 to 1,600, where 80.2% of the positive samples had titers

This study aimed to evaluate the yield and physicochemical characteristics of three West Indian Cherry genotypes cultivated in the semi-arid region of the state of Piauí, Brazil. The experiment was conducted in a randomized block design (RBD) with three replications and three treatments, corresponding to the West Indian Cherry genotypes ‘Clone 14’, ‘BRS 366 Jaburu’, and ‘Junko’ cultivated in a 4 x 3 m spacing. Combined genotype analysis revealed the following mean variations: yield from 22.96 to 47.53 t ha -1; fruit mass from 4.18 to 5.52 g; longitudinal diameter from 20.28 to 22.80 mm; transverse diameter from 17.32 to 18.42 mm, pulp yield from 27.58 % to 34.54%; red Hue° varying from 19.00° to 26.00°; soluble solids from 7.2 to 8.1 °Brix; titratable acidity from 0.82 to 1.34; ratio of total soluble solids to titratable acidity from 5.00 to 10.37; pH from 3.52 to 3.74; total anthocyanins from 2.56 to 12.11 mg.100 g-1 of pulp; flavonoids from 4.30 to 7.44 mg.100 g-1 of pulp; lycopene from 0.30 to 4.71 mg.100 g-1 of pulp; β-carotene from 14.00 to 32.51 mg 100 g-1 of pulp; mean ascorbic acid content of 2.676 mg.100 g-1 of pulp. Under the present experimental conditions, “BRS 366 Jaburu” was the most promising among the studied genotypes. Made available in DSpace on 2022-10-04T17:05:54Z (GMT). No. of bitstreams: 1 Yield-and-physicochemical-characteristics-of-west-indian-cherry-2021.pdf: 194786 bytes, checksum: db79017efe819066e30dcbfe4fffdfba (MD5) Previous issue date: 2021

Cajui (Anacardium spp.) is a native fruit tree (small cashew) of the Brazilian Cerrado and possesses the potential for commercialization. However, cajui exploitation occurs exclusively through extractivism in the absence of conservation strategies. The lack of conservation strategies may lead to a decrease in genetic diversity of Anacardium. In this work, the genetic diversity and population structure of three natural populations in Sete Cidades National Park (PNSC; PI, Brazil) were assessed using ISSR analysis of 56 cajui accessions and two A. occidentale accessions (outgroup) from Pacajus (CE, Brazil). A total of 112 markers were obtained, 93 (83.04%) of which were polymorphic. Made available in DSpace on 2022-01-26T13:00:30Z (GMT). No. of bitstreams: 1 Bioscience-Journal.pdf: 624978 bytes, checksum: f4f34e1b0ea05dce52d31d4ef8bcab9b (MD5) Previous issue date: 2021

International audience; We consider the constrained multi-objective optimization problem of finding Pareto critical points of difference of convex functions. The new approach proposed by Bento et al. (SIAM J Optim 28:1104–1120, 2018) to study the convergence of the proximal point method is applied. Our method minimizes at each iteration a convex approximation instead of the (non-convex) objective function constrained to a possibly non-convex set which assures the vector improving process. The motivation comes from the famous Group Dynamic problem in Behavioral Sciences where, at each step, a group of (possible badly informed) agents tries to increase his joint payoff, in order to be able to increase the payoff of each of them. In this way, at each step, this ascent process guarantees the stability of the group. Some encouraging preliminary numerical results are reported.

Beans from cowpea cultivars fertilized with mineral N or inoculated with various rhizobium strainsmay contain different nitrogen concentrations and nitrogen metabolite composition, which affects the beans? defense mechanisms against pests. In this study, the population growth of Callosobruchus maculatus reared on beans from four cowpea cultivars fertilized with different nitrogen sources was evaluated. The factors tested were beans from four cowpea cultivars and seven different nitrogen sources: mineral N fertilization, inoculation with five strains of symbiotic diazotrophic bacteria, and soil nitrogen (absolute control).

International audience; We propose an inertial proximal point method for variational inclusion involving difference of two maximal monotone vector fields in Hadamard manifolds. We prove that if the sequence generated by the method is bounded, then every cluster point is a solution of the non-monotone variational inclusion. Some sufficient conditions for boundedness and full convergence of the sequence are presented. The efficiency of the method is verified by numerical experiments comparing its performance with classical versions of the method for monotone and non-monotone problems.

International audience; In this paper we present a subgradient method with non-monotone line search for the minimization of convex functions with simple convex constraints. Different from the standard subgradient method with prefixed step sizes, the new method selects the step sizes in an adaptive way. Under mild conditions asymptotic convergence results and iteration-complexity bounds are obtained. Preliminary numerical results illustrate the relative efficiency of the proposed method.

In poultry, the reduction in genetic variability of native chicken populations has led to the use of microsatellites in many genetic studies of chicken ecotypes. To be of maximum usefulness as a genetic marker, microsatellite primers should be amplifying the same locus other than the source of the primer sequence in different populations. Even in closely related lines or breeds microsatellite genotyping errors may be introduced from primer mismatches as a result of mutations in the primer binding sites. Therefore, the selection, use and optimization of microsatellites are considered to be a fundamental step towards full success in genetic studies. Herein, 20 microsatellite loci are presented with great potential for diversity studies in Brazilian chicken ecotypes. The analyses of these ecotypes revealed a total of 191 robust alleles, ranging from three to 18, with an average of 9.6 alleles per locus. The average observed heterozygosity was 0.785, while the mean expected heterozygosity was 0.688. Additionally, the mean polymorphic information content value (0.731) further reflected high level of polymorphism across all microsatellite loci. The topology of the dendrogram constructed with the neighbour-joining method showed probable patterns of relationship and genetic differentiation among the individual ecotypes. Overall, microsatellite loci have proven to be highly useful for studying the variability of chicken ecotypes in the Mid-North region of Brazil.

Made available in DSpace on 2021-06-25T10:29:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 This study aimed to assess the growth, nutrition and visual deficiency symptoms of cucumber plants as a function of macronutrient omission. The experiment was conducted in a completely randomized design, with seven treatments and four replicates, corresponding to nutrient solution without nutrient omission (CS) of Hoagland and Arnon, nitrogen (−N), phosphorus (−P), potassium (−K), calcium (−Ca), magnesium (−Mg) and sulfur (−S) omission. Omissions decreased the accumulation of macronutrients with detrimental effects in physiological variables and in the efficiency of absorption, transport and use of macronutrients, causing losses in growth and on dry mass production. Since the losses in dry mass in the shoot were up to 85%, 85%, 83%, 47%, 40% and 40% to the omission of Ca, Mg, N, P, K and S, respectively. The accumulation of macronutrients in the aerial part of cucumber plants that received the complete solution (CS) in relation to each omission (−) was 1058/75, 157/20, 958/152, 578/9, 147/5 and 142/12 for CSN/−NN, SCP/−PP, SCK/−KK, SCCa/−CaCa, SCMg/−MgMg and SCS/−SS, respectively. Therefore, the lowest macronutrients accumulation, the green color index, plant growth and dry matter production were reduced in cucumber plants. The order of limitation of macronutrients in the shoot dry matter production in cucumber plants was N = Ca = Mg > P > K = S. Federal University of Mato Grosso do Sul (UFMS) Laboratory of Plant Nutrition Department of Soils and Fertilizers São Paulo State University (UNESP) Department of Agronomy Mato Grosso State University (UNEMAT) Department of Agronomy Federal University of Piauí (UFPI) Laboratory of Plant Nutrition Department of Soils and Fertilizers São Paulo State University (UNESP)

Made available in DSpace on 2021-06-25T10:47:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Casearia sylvestris is an outstanding representative of the Casearia genus. This representability comes from its distinctive chemical profile and pharmacological properties. This species is widespread from North to South America, occurring in all Brazilian biomes. Based on their morphology, 2 varieties are recognized: C. sylvestris var. sylvestris and C. sylvestris var. lingua. Despite the existence of data about their chemical composition, a deeper understanding of the specialized metabolism correlation and variation in respect to environmental factors and its repercussion over their biological activities was still pending. In this study, an UHPLC-DAD-based metabolomics approach was employed for the investigation of the chemical variation of 12 C. sylvestris populations sampled across 4? Brazilian biomes and ecotones. The correlation between infraspecific chemical variability and the cytotoxic and antioxidant activities was achieved by multivariate data analysis. The analyses showed that C. sylvestris var. lingua prevailed at Cerrado areas, and it was correlated with lower cytotoxic activity and high level of glycosylated flavonoids. Among them, narcissin and isorhamnetin-3- O-α -L-rhamnopyranosyl-(1 → 2)- α -L-arabinopyranoside showed good correlation with the antioxidant activity. Conversely, C. sylvestris var. sylvestris prevailed at the Atlantic Forest areas, and it was associated with high cytotoxic activity and high content of clerodane diterpenoids. Different casearins showed good correlation (R 2= 0.3?-?0.70) with the cytotoxic activity. These findings highlighted the great complexity among different C. sylvestris populations, their chemical profile, and the related biological activities. Consequently, it can certainly influence the medicinal properties, as well as the quality and efficacy, of C. sylvestris phytomedicines. Institute of Chemistry Department of Biochemistry and Organic Chemistry São Paulo State University (UNESP), CEP 14800-060, Rua Prof. Francisco Degni 55 Natural Products Laboratory Institute of Biology Leiden University Natl. Inst. of Alternative Technol. for Detection Toxicological Assess. Removal of Micropollutants and Radioactive Substances Araraquara Agronomic Institute of Campinas Herbarium IAC Department of Biophysics and Physiology Laboratory of Experimental Cancerology Federal University of Piauí Department of Physiology and Pharmacology Faculty of Medicine Federal University of Ceará Institute of Chemistry Department of Biochemistry and Organic Chemistry São Paulo State University (UNESP), CEP 14800-060, Rua Prof. Francisco Degni 55

Made available in DSpace on 2020-12-12T01:29:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Despite the importance of nitrogen (N) supply to plants, there are still doubts concerning the optimal relations of ammonium and nitrate in the nutrition of yellow passion fruit seedlings. This study aims to evaluate the interaction between nitrogen concentrations and ammonium and nitrate proportions in the nutrition, growth, and dry matter production of passion fruit seedlings grown in a substrate with a nutrient solution. The experiment was conducted in a greenhouse in randomized complete block design with three replications in a 4 × 5 factorial design, consisting of four N concentrations (2.5, 5.0, 10.0 and 20.0 mmol L−1) and five ammonium proportions (0, 25, 50, 75 and 100% in relation to the total N supply). At 60 days after transplanting, green color index; accumulation of N, potassium, calcium, and magnesium in roots and shoots; stem diameter; leaf area; root length; nitrogen use efficiency (NUE); and dry matter of roots and shoots were evaluated. For the formation of seedlings of yellow passion fruit, the nutrient solution should have 13 mmol L−1 of N, with 40% of this nutrient in the form of ammonium. The passion fruit is a plant tolerant to ammonium. However, a critical concentration above 5.7 mmol L−1 of NH4+ in the nutrient solution decreases absorption of cations, NUE, and production of dry matter. Department of Plant Science Research Center of Agricultural Sciences Campus of Ministro Petrônio Portella Federal University of Piauí Department of Soils and Fertilizers Faculty of Agriculture and Veterinary Sciences São Paulo State University São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences Department of Agronomy Federal Institute of Maranhão Department of Agronomy Federal University of Mato Grosso do Sul Campus Chapadão do Sul Department of Agronomy Universidad de Pamplona Paulo Donato Castellane Department of Soils and Fertilizers Faculty of Agriculture and Veterinary Sciences São Paulo State University São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences

Made available in DSpace on 2020-12-12T02:08:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Knowledge of the spatial variability of compaction would minimize the movement of soil and optimize the operation and economic viability of sugarcane activities. The present study aimed to evaluate different protocols for soil loosening, using subsoiling at fixed and varying depths. The following four protocols were assessed: (i) total area subsoiling with a 7-shank sub-soiler at a fixed depth of 0.4 m in both row and inter-row zones; (ii) total area subsoiling with a 7-shank sub-soiler at varying depths in both row and inter-row zones; (iii) localized subsoiling with a 4-shank sub-soiler at a fixed depth of 0.4 m in a row; and (iv) localized subsoiling with a 4-shank sub-soiler at varying depths in a row. Soil resistance to penetration was measured at 111 sites within a 10 ha area, at a depth of 0.4 m. Data were subjected to descriptive and geostatistical analyses. Localized subsoiling at varying depths had an operational capacity 61.7% greater than the total area operation at a fixed depth. Moreover, along with producing a savings of 57.1% for diesel oil, the emission of equivalent CO2 was reduced by approximately the same amount. Mapping based on site-specific management presented good sustainability indicators, such as reduced energy consumption and lower CO2 emission. Department of Soils and Fertilizers Research Group CSME - Soil Characterization for Specific Management Campus in Jaboticabal (UNESP-FCAV) College of Agricultural and Veterinary Sciences São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n Federal University of Piauí, Campus Cinobelina Elvas, Rodovia Municipal Bom Jesus-Viana, km 01, Planalto Horizonte, s/n Department of Rural Engineering Campus in Jaboticabal (UNESP-FCAV) College of Agricultural and Veterinary Sciences São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n Department of Soils and Fertilizers Research Group CSME - Soil Characterization for Specific Management Campus in Jaboticabal (UNESP-FCAV) College of Agricultural and Veterinary Sciences São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n Department of Rural Engineering Campus in Jaboticabal (UNESP-FCAV) College of Agricultural and Veterinary Sciences São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n

Made available in DSpace on 2022-04-28T19:47:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 The objective of this survey was to determine the seroprevalence of Leptospira spp., the most frequent serogroups, and the geospatial distribution in goats in seven states in the Northeast region of Brazil. Samples (n = 4,718) were collected in the states of Alagoas, Ceará, Maranhão, Paraíba, Piauí, Rio Grande do Norte and Sergipe and were analyzed using the microscopic agglutination test (MAT) with a cut-off point of 1:100. Additionally, geographic data were analyzed to construct epidemiological maps for characterization of leptospirosis in the municipalities with greater importance for goat farming in each state. Of the sampled animals, 17.7% were serologically reactive for Leptospira spp. Animal-level prevalence ranged within the states, from 7.07% (Alagoas) to 30.23% (Paraíba) with statistical difference (P < 0.001). For herds, 83.4% had at least one serologically reactive animal. Paraíba had the highest herd-level prevalence (98.36%) and Piauí the lowest (65.91%) (P < 0.001). The states of Ceará, Rio Grande do Norte, and Sergipe had 91.18%, 69.64%, and 92.59% of positive herds, respectively. The most frequently identified serogroups were Autumnalis (23.06%) and Australis (20.98%) and titers ranged from 100 to 1,600. In conclusion, leptospirosis is present in states in the Northeast region of Brazil, especially in environments with dry and deforested areas, demonstrating a possible adaptation of the infectious agent to areas of adverse climates and an apparent interspecies transmission. Also, it was possible to verify the circulation of serogroups that may be adapted to small ruminants maintained in semiarid conditions, causing the asymptomatic disease with reproductive losses. Transmissible Disease Research Group Federal University of Campina Grande Postgraduate Program in Animal Science Federal University of Piauí Brazilian Agricultural Research Corporation Embrapa Caprinos e Ovinos Department of Veterinary Sciences UFPB Federal University of Paraíba Postgraduate Program in Animal Bioscience University of Cuiabá State University of São Paulo

Made available in DSpace on 2022-04-28T19:49:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 We sought to investigate the acute effects of capsaicin supplementation on the rate of perceived exertion (RPE) and heart rate during high-intensity intermittent exercise (HIIE) and resistance exercise performance executed after HIIE in resistance-trained men. Eleven resistance-trained men completed 2 randomized, double-blind trials: capsaicin condition or a placebo condition. The concurrent exercise session was composed of a 5-km intermittent run (1:1 effort and passive recovery ratio) and subsequent resistance exercise (4 × 70% of 1RM until muscle failure in the half-squat exercise). Heart rate was recorded during HIIE and after the protocol RPE (0–10 scale) was accessed. The resistance exercise performance was analyzed by the maximum number of repetitions performed for each set and the total volume (repetitions 3 weight lifted). During HIIE, the RPE (capsaicin = 7 ± 1 vs. placebo = 8 ± 1 points, t = -3.674, p = 0.005) and mean heart rate (capsaicin = 153 ± 13 vs. placebo = 158 ± 12 bpm, t = -2.292, p = 0.048) were significantly lower in the capsaicin compared with placebo condition. For subsequent resistance exercise, there was a significant decrease in volume across time (F = 19.889, p< 0.001, η2 = 0.69) with maximal number of repetitions performed in capsaicin than placebo condition (2,077.6 ± 465.2 kg vs. 1,838.9 ± 624.1 kg, p = 0.028, d = 0.43), but the condition vs. set was not significant (F = 0.582, p = 0.632, η2 = 0.06). Acute capsaicin supplementation induced a lower mean heart rate and RPE during HIIE and improved subsequent resistance exercise performance in resistance-trained men. Skeletal Muscle Assessment Laboratory (LABSIM) Department of Physical Education School of Technology and Sciences São Paulo State University (UNESP) Department of Physical Education Center University of Adamantina (UNIFAI) Department of Nutrition University of Western São Paulo (UNOESTE) Department of Kinesiology Coastal Carolina University School of Physical Education and Sport University of São Paulo Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP), SP Immunometabolism of Skeletal Muscle and Exercise Research Group Department of Physical Education Federal University of Piauí (UFPI), PI Associate Graduate Program in Health Science Federal University of Piauí (UFPI), PI Skeletal Muscle Assessment Laboratory (LABSIM) Department of Physical Education School of Technology and Sciences São Paulo State University (UNESP) Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP), SP

Made available in DSpace on 2022-05-01T11:07:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The production of edible film from onion (Allium cepa L.) to be applied as packaging is attractive, due to its chemical properties and biodegradable characteristics. Thus, we tested the hypothesis that edible onion film can positively influence the sensory properties, quality and increasing shelf life of beef burgers patties. The experiment was designed in a 4 × 2 factorial scheme, with two treatments (beef burgers patties with or without edible onion film) at an interval of four storage times (0, 3, 6 and 9 days) at 4◦ C. The uncoated burger patties (control) suffered the most intense color modifications during the storage (p < 0.05). The luminosity index was higher (p < 0.05) in the control at all storage times, except at day 6, and redness, yellowness and chrome were higher (p < 0.05) in the edible onion film patties at all storage times. The pH of the beef burger patties was lower (p < 0.05) at all storage times when the edible onion film was applied. For the texture profile, only the chewiness was affected, as the inclusion of the edible onion film improved the chewing of the beef burgers patties over the storage time (p < 0.05). Additionally, there was an inhibition of the microbial growth of mesophiles and psychrophiles with the application of the edible onion film in beef burgers patties. The use of edible onion film improved the perception of panelists for the variables texture, color, flavor, odor and overall appearance, and increased the preference of panelists. The edible onion film is recommended for preserving beef burgers patties, as it delays the proliferation of unwanted microorganisms, stabilizes and improves the color parameters and sensory attributes, and increases the overall acceptance of the consumer. Department of Animal Science Animal Health and Science Graduate Federal University of Campina Grande Chemistry Department Laboratory of Advanced Materials Federal University of Piauí (UFPI) Department of Biotechnology University Center of Araraquara (UNIARA) Chemical Institute São Paulo State University (UNESP) BioSmart Nano Technology Health Department Federal Institute of Education Science and Technology of Paraiba/Campus Patos Department of Animal Science Federal University of Bahia Chemical Institute São Paulo State University (UNESP) FAPESP: 16/15504-2

Made available in DSpace on 2022-04-28T19:46:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-15 Cowpea [Vigna unguiculata (L.) Walp] is cultivated in tropical and subtropical regions worldwide, but its production is usually limited by boron (B) deficiency, which can be mitigated by applying B via foliar spraying. In plants with nutrient mobility, the residual effect of foliar fertilization increases, which might improve its efficiency. An experiment was carried out to evaluate the concentration and mobility of the B isotopic tracer (10B) in different organs of cowpea plants, after the application of this micronutrient in the growing media and also to leaves. Treatments were designed based on B fertilization as follows: without B in the growth media, with 10B applied via foliar spraying (10B-L), with B in the growth media (substrate) and 10B via foliar spraying (10B-L + B-S), and with 10B in the growth media (substrate) without foliar spraying (10B-S), and a control without fertilization. A redistribution of 10B was observed in new leaves when the element was supplied via foliar spraying, resulting in greater leaf area, dry mass and dry matter production of aerial parts, and also the whole plant. 10Boron was redistributed when applied via foliar spraying in cowpea plants, regardless of the plant's nutritional status, which in turn might increase internal B cycling. Department of Agronomy Federal Institute of Maranhão Department of Agricultural Production Sciences Soils and Fertilizers Sector São Paulo State University (UNESP) Center for Nuclear Energy in Agriculture Universidade de São Paulo (USP) Soil Department State University of Mato Grosso (UNEMAT) Department of Plant Science Research Center of Agricultural Sciences Federal University of Piauí Department of Agricultural Production Sciences Soils and Fertilizers Sector São Paulo State University (UNESP)

Made available in DSpace on 2020-12-12T01:14:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 The purpose of present study was to compare the effects of moderate-load versus high-load resistance exercise (RE) on hunger response, blood lactate, glucose, and autonomic modulation in trained men, and to examine the correlations between these parameters. For this, 11 recreationally resistance-trained males performed 2 randomized trials: moderate-load (6 sets at 70% 1-repetition maximum (1RM) and a 90-s rest interval between sets) and high-load (6 sets at 90% 1RM and a 180-s rest interval between sets) leg-press exercise until movement failure. The subjective rating of hunger was obtained through a visual analog scale. Glucose and lactate concentration were evaluated at rest, immediately after exercise, and 30 min after exercise. Heart rate variability was recorded at baseline and during recovery (until 30 min after exercise) to assess autonomic modulation. The moderate-load condition induced lower subjective hunger ratings than the strength condition immediately after exercise (19.7 ± 16.6 vs 47.3 ± 27.7 mm), 30 min after exercise (33.6 ± 22.9 vs 58.5 ± 29.9 mm), and 60 min after exercise (43.8 ± 26.6 vs 67.8 ± 27.9 mm) (p < 0.05) and lower area under the curve hunger in relation to the high-load condition (p < 0.006). Moderate-load RE presented greater lactate concentration and induced slower heart rate variability recovery in relation to high-load RE (p < 0.05), but no difference was found in glucose, as well as no correlations between any of the variables investigated. In conclusion, moderate-load RE induced lower subjective hunger ratings, slower recovery of the parasympathetic nervous system, and higher lactate concentration in relation to high-load RE, but the metabolic variables were not correlated with hunger suppression. Skeletal Muscle Assessment Laboratory (LABSIM) Department of Physical Education School of Technology and Sciences São Paulo State University (UNESP) School of Technology and Sciences São Paulo State University (UNESP) Department of Nutrition University of Western São Paulo (UNOESTE) School of Physical Education and Sport University of São Paulo Department of Biomedicine University of Western São Paulo (UNOESTE) Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP) Immunometabolism of Skeletal Muscle and Exercise Research Group Federal University of Piauí (UFPI) Federal University of Piauí (UFPI) Skeletal Muscle Assessment Laboratory (LABSIM) Department of Physical Education School of Technology and Sciences São Paulo State University (UNESP) School of Technology and Sciences São Paulo State University (UNESP) Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP)

Made available in DSpace on 2020-12-12T02:46:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Composting has been recommended as a suitable alternative for recycling wastes and could improve tannery sludge (TS) before its use. However, the long-term application of composted tannery sludge (CTS) may bring concerns about its effects on soil properties and, consequently, on plants and environment, mainly when considering Cr contamination. In this study, we summarize the responses of soil chemical and biological parameters in a 10-year study with yearly applications of CTS. Chemical and biological parameters were assessed in soil samples, and the multivariate analysis method principal response curve (PRC) was used to show the temporal changes in all the biological and chemical properties caused by CTS. The PRC analysis showed different long-term response patterns of chemical and biological parameters according to the rates of CTS. Interestingly, Cr content increased strongly in the first 5 years and only increased slightly in the following 5 years. The yearly applications of CTS changed the biological and chemical parameters of the soil, negatively and positively, respectively. Organic matter, K and P, increased during the 10 years of application, while soil pH and Cr concentration increased, and soil microbial biomass and enzymes activity decreased. Soil Quality Lab. Agricultural Science Center Federal University of Piauí School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP) University of Brazil Campus Descalvado, Hilário da Silva Passos Avenue, 950, University Park University of West São Paulo UNOESTE Wageningen Environmental Research Aquatic Ecology and Water Quality Management Group Wageningen University School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)

Introduction: In a saturated market with an over-supply of undergraduate and graduate programs, social media have become attractive means of advertising in Dentistry. However, posts frequently contain ethical violations and lead to service commodification, and their contents are often in disagreement with the Code of Consumer Protection. Objective: This article, which focuses on ethical and legal developments, contributes to the discussion and elucidation of questions associated with advertising that uses clinical images and photographs of patients in social media for commercial purposes and self-promotion. Conclusion: Social media and networks are valuable tools of dissemination and exchange of information because of their flexibility, democratic character and low cost. However, their abusive or misleading use, as well as the ethical and legal violations associated with the difficulty in controlling their use, may lead to serious damages and unfavorable court decisions. RESUMO Introdução: diante de um mercado saturado e com excesso de oferta de cursos de graduação e pós-graduação, as mídias sociais tornaram-se um atrativo veículo para publicidade em Odontologia. Entretanto, são frequentes as postagens com violações éticas, mercantilização e conteúdo em desacordo com o Código de Defesa do Consumidor. Objetivo: o presente artigo tem como objetivo contribuir para a discussão e elucidação das questões relativas à publicação de imagens clínicas e de pacientes nas mídias sociais, com o intuito comercial e autopromoção, enfocando os desdobramentos éticos e legais. Conclusão: as mídias/redes sociais são valiosas ferramentas de divulgação e troca de informação, por sua agilidade, caráter democrático e baixo custo. Contudo, sua utilização de forma abusiva e enganosa, com infrações éticas e legais, associada à dificuldade de fiscalização, pode trazer sérios prejuízos, resultando em jurisprudências desfavoráveis.

Made available in DSpace on 2019-10-06T17:04:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-06-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The aim of this study was to select bacterial strains with biochemical capability to tolerate high concentration of chromium (Cr) in soils. Plant growth-promoting bacteria (PGPB) were isolated from the root nodules of Phaseolus lunatus and grown in Cr-contaminated soil with the application of composted tannery sludge. Soils were collected from the experimental field with the application of composted tannery sludge (CTS) in five rates: 0, 2.5, 5, 10, and 20 t ha−1 CTS. Bacterial strains were isolated and evaluated for their biochemical capabilities for production of urease, protease, amylase, lipase, catalase, gelatinase, and indole-3-acetic acid, P solubilization, and Cr tolerance. A total of 54 PGPB were isolated from the nodules, being 40%, 37%, 13%, and 10% found in the treatments with 2.5, 5, 10, and 20 t ha−1, respectively. The majority of these isolates presented positive responses for the tests of urease, catalase, and phosphate solubilization, while some isolates were positive for the test of protease, lipase, carboxymethyl cellulose, gelatinase, and amylase. We also observed a decrease in the number of isolates able to tolerate high concentration of Cr. Three strains (UFPI-LCC61, UFPI-LCC64, and UFPI-LCC87) presented high biochemical capability and tolerance to Cr. However, the isolate UFPI-LCC87 showed high biochemical capability and tolerance to the highest concentration of Cr. Our results indicated bacterial strains that present potential to be used in soils contaminated with Cr and also for promoting plant growth. Soil Quality Lab. Agricultural Science Center Federal University of Piauí School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), Prof. Paulo Donato Castellane, Km 5 University of Brazil: University campus Descalvado, Hilário da Silva Passos Avenue, 950 - University park Cell and Molecular Biology Laboratory Center for Nuclear Energy in Agriculture CENA University of Sao Paulo USP School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), Prof. Paulo Donato Castellane, Km 5 CNPq: 201005/2014-0

Background: Several studies proved that anodic oxidation improves osseointegration. This study aimed to optimize osseointegration through anodization in dental implants, obtaining anatase phase and controlled nanotopography. Methods: The division of the groups with 60 titanium implants was: control (CG), sandblasted (SG), anodized (AG): anodized pulsed current (duty cycle 30%, 30 V, 0.2 A and 1000 Hz). Before surgery, surface characterization was performed using Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), X-ray Dispersive Energy Spectroscopy (EDS) and Raman Spectroscopy. For in vivo tests, 10 New Zealand white rabbits received an implant from each group. The sacrifice period was 2 and 6 weeks (n = 5) and the specimens were subjected to computed microtomography (μCT) and reverse torque test. Results: AFM and SEM demonstrated a particular nanotopography on the surface in AG, the anatase phase was proved by Raman spectroscopy. In the μCT and in the reverse torque test, the AG group presented better results than the other groups. Conclusion: The chemical composition and structure of the TiO2 film were positively affected by the anodizing technique, intensifying the biological characteristics in osseointegration.

Made available in DSpace on 2021-06-25T10:26:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Additives used to improve feed efficiency of beef cattle on high-grain diets requires products that not only increase animal performance but also provide food safety for consumers. Since phytogenic additives such as essential oils (EO) are the main substitutes for monensin in the diet of cattle fed high-grain diets, this study aimed to evaluate, through meta-analysis, the effects of EO as an alternative to monensin in diets for beef cattle on feed intake, performance, carcass characteristics and ruminal fermentative parameters. Ten peer-reviewed publications with 27 treatment means were included in the data set. These effects were evaluated using random-effect models to examine the weighted mean differences (WMD) between EO treatment and control treatment (diets with monensin). Heterogeneity was explored by meta-regression and subgroup analysis. The substitution of EO for monensin did not affect methane production, ruminal pH values, average daily gain, feed efficiency or carcass weight. However, carcass dressing percentage (WMD = 0.38%; P = 0.03), ribeye area (WMD = 0.82 cm2; P < 0.0001) and subcutaneous fat thickness (WMD = 0.56 mm; P < 0.0001) values increased. Although the use of EO instead of monensin had no influence on the performance of beef cattle fed high-grain diets, the prevalence of hepatic abscesses increased 84.9% and the replacement of monensin by EO increased the risk ratio of hepatic abscess prevalence by 107%. Therefore, the use of EO in high-grain beef cattle diets was ineffective in protecting the liver against abscesses. Animal Unit of Digestive and Metabolic Studies Department of Animal Science School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) Federal University of Piauí Department of Biological Safety German Federal Institute for Risk Assessment Federal University of Espírito Santo Department of Animal Science Animal Unit of Digestive and Metabolic Studies Department of Animal Science School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)

Made available in DSpace on 2022-05-01T08:45:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This meta-analysis was carried to evaluate the effect of the use of crude glycerin in diets for beef cattle on the ruminal fermentation, animal performance, physical and chemical characteristics, and fatty acid profile of meat through meta-analysis. Data from forty-eight peer-reviewed publications with 170 treatments means was included in the data set. The effect glycerin in diet were evaluated by examining the weighted mean differences (WMD) between glycerin treatment (diets with crude glycerin) and control diet (without crude glycerin). Heterogeneity was explored by meta-regression and subgroup analysis using genetic type, treatment period, crude glycerin in the diet (g/kg DM), feed systems (pasture or total mixed ration), and concentrate in the diet (g/kg DM). The inclusion of crude glycerin had no effect on the average daily gain, but increased feed efficiency by 3.15% while reducing subcutaneous fat thickness by 3.13%. Inclusion of crude glycerin reduced meat cholesterol by 9.13%, and total saturated fatty acids by 1.05%, and increased total unsaturated (2.02%), monounsaturated (3.17%) fatty acids. However, it did not affect the concentrations of conjugated linoleic acid and omega-3. Crude glycerin inclusions up to 200 g/kg DM did not promote a negative effect on animal performance, carcass and physical-chemical characteristics of meat, and supported an increase in total monounsaturated (1.73%), oleic acid (12.29 mg) and palmitoleic acid (1.24 mg), while reducing myristic acid (3.08 mg), stearic acid (12.00 mg) in beef cattle meat. Animal Unit of Digestive and Metabolic Studies in the Department of Animal Science School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP) Federal University of Piauí Federal University of Espírito Santo Departament of Animal Science Animal Unit of Digestive and Metabolic Studies in the Department of Animal Science School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)

Made available in DSpace on 2021-06-25T10:24:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Citrus companies have sought and developed alternative systems of tillage or implanting orchards so as not to significantly alter the physical and chemical attributes of the soil and, consequently, the root development of plants. Therefore, the aim of this study was to identify the physical and chemical attributes of the soil that most influence the root volume of the orange crop in different tillage systems. The experiment was carried out in the region of Avaré, state of São Paulo, Brazil, in Utissol and Oxisol. For the planting of the orange crop, the following tillages were made: minimum tillage, subsoiler tillage and soil tillage using a triple tillage implement. The physical and chemical attributes evaluated were bulk density, macroporosity, microporosity, total porosity, soil moisture, soil mean weight-diameter, soil resistance to penetration, sum of bases, cation exchange capacity, base saturation, pH, exchangeable cations, potential acidity, available phosphorus, analysis of mi-cronutrients such as copper, iron, manganese and zinc, and organic carbon content. The root system was evaluated using SIARCS® software. For data classification, data mining techniques were used such as attribute selection and decision tree induction. Regardless of the soil type, the use of the triple operation implement provided greater root volume for orange plants. For the Utisol area, the pH value of 4.2 was the main attribute that provided a high root volume. For the Oxisol, the presence of copper, in levels that did not generate toxicity for the plants, provided a high volume of root for the crop. Campus Cinobelina Elvas-Av. Manoel Gracindo Federal University of Piauí (UFPI), km 01, Planalto Horizonte, Bom Jesus School of Agricultural Engineering (FEAGRI) University of Campinas (UNICAMP), Av. Cândido Rondon, 501, Barão Geraldo School of Agricultural and Veterinarian Sciences (FCAV) State University (UNESP), Street Professor Paulo Donato Castelane, S/N, Vila Industrial School of Agricultural and Veterinarian Sciences (FCAV) State University (UNESP), Street Professor Paulo Donato Castelane, S/N, Vila Industrial CAPES: 001 FAPESP: 2010/14850–8

Made available in DSpace on 2022-04-28T19:41:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Background: Acute capsaicinoid and capsinoid supplementation has endurance and resistance exercise benefits; however, if these short-term performance benefits translate into chronic benefits when combined with resistance training is currently unknown. This study investigated changes of chronic Capsiate supplementation on muscular adaptations, inflammatory response and performance in untrained men. Methods: Twenty untrained men were randomized to ingest 12 mg Capsiate (CAP) or placebo in a parallel, double-blind design. Body composition and performance were measured at pre-training and after 6 weeks of resistance training. An acute resistance exercise session test was performed pre and post-intervention. Blood samples were collected at rest and post-resistance exercise to analyze Tumor necrosis factor- (TNF-), Soluble TNF- receptor (sTNF-r), Interleukin-6 (IL-6) and Interleukin-10 (IL-10). Results: Exercise and CAP supplementation increased fat-free mass in comparison to baseline by 1.5 kg (P < 0.001), however, the majority of the increase (1.0 kg) resulted from an increase in total body water. The CAP change scores for fat-free mass were significantly greater in comparison to the placebo (CAP ∆%= 2.1 ± 1.8 %, PLA ∆%= 0.7 ± 1.3 %, P = 0.043) and there was a significant difference between groups in the bench press exercise (P = 0.034) with greater upper body strength change score for CAP (∆%= 13.4 ± 9.1 %) compared to placebo (∆%= 5.8 ± 5.2 %), P = 0.041. CAP had no effect on lower body strength and no supplementation interactions were observed for all cytokines in response to acute resistance exercise (P > 0.05). Conclusion: Chronic Capsiate supplementation combined with resistance training during short period (6 weeks) increased fat-free mass and upper body strength but not inflammatory response and performance in young untrained men. Immunometabolism of Skeletal Muscle and Exercise Research Group Department of Physical Education Federal University of Piauí (UFPI), “Ministro Petrônio Portella” Campus Department of Exercise Physiology University of Lynchburg Increnovo LLC, 2138 E Lafayette Pl Laboratory of Cellular and Molecular Biology of Skeletal Muscle (LABCEMME) Department of Physical Education Federal University of Maranhão Department of Nutrition University of Western São Paulo (UNOESTE) Department of Biophysics and Physiology Federal University of Piaui, Campus Minister Petrônio Portela Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP) University São Judas Tadeu Associate Professor at Graduation Program in Science and Health Federal University of Piaui (UFPI) Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP)

Made available in DSpace on 2022-04-28T19:44:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The aim of this study was to evaluate the quality of Paredão grass silage (Panicum maximum Jacq.) with fermented juice produced from corn silage in different fermentation periods, as bioinoculant, through the characteristics: gas and effluent losses, dry matter recovery, chemical composition, microbiological profile and aerobic stability. The treatments consisted of fermented corn silage juices obtained in different fermentation periods, which were: 0, 5, 10, 15 and 30 days of fermentation, and were used as a bioinoculant additive in Paredão grass silage. The lowest effluent losses were observed in silages containing corn silage juice fermented for 10 days, while the highest dry matter recovery values were observed in silages containing corn silage juice fermented for 30 days. The highest values of crude protein were observed in silages containing corn silage juice fermented for 10 days. The highest content of lactic acid (53 ± 2.2 g/kg DM) was observed in the Paredão grass silage containing corn silage juice fermented for 10 days. The inclusion of corn silage juice fermented for 10 days as bioinoculant improves the quality of Paredão grass silage, as it presented greater recovery of the silage dry matter, higher crude protein content and greater amount of beneficial acids São Paulo State University (Unesp) Federal University of Piauí Federal University of Campina Grande São Paulo State University (Unesp)

Made available in DSpace on 2022-04-28T17:20:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Federal University of Piaui This study aimed to evaluate the agronomic parameters and the chemical composition of the cactus pear variety Doce (Nopalea cochenillifera) in different Brazilian savannah localities, subjected to different nitrogen fertilization strategies. A randomized block design was used in this experiment, with a 2x 4 x 2 factorial arrangement with five replications. The factors corresponded to two application frequencies of nitrogen fertilization (30 days and 30/60 days), four nitrogen doses (0, 100, 200, 400 kg ha(-1) year(-1)), and two cactus pear cultivation localities, with Locality 1 corresponding to the municipality of Curimata and Locality 2 corresponding to the municipality of Riacho Frio. After one year of cultivation, there was no interaction (P>0.05) for frequency x doses x locality, doses x locality, and frequency x doses. The means observed for fresh and dry matter production varied from 57.0 to 68.0 +/- 2.9 t ha(-1) and from 2.4 to 3.7 +/- 0.2 t ha(-1), respectively. There was an interaction (P

Made available in DSpace on 2021-06-25T10:58:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Purpose: Regular physical activity is a good strategy to maintain the health of athletes, and prevent pain and decreased joint flexibility during the pandemic. On the other hand, higher sedentary time during the pandemic period can have deleterious effects. The objective of this study was to compare physical activity levels, sedentary time, and sleep parameters during the pre-COVID period and the COVID-19 pandemic period in young badminton athletes. Methods: Fifteen young badminton athletes were evaluated during a pre-COVID period (July 2019) and during the COVID-19 period (July 2020). Sleep parameters, physical activity level, and sedentary time were measured using a tri-axial accelerometer. Participants wore the accelerometer on their dominant wrist for 7 days consecutively. In addition, the average of each sleep parameter [time in bed and total sleep time in hours per day, sleep efficiency (%), wake after sleep onset (WASO, total per day), and sleep latency (minutes per day)] was reported over the 7-day period. Results: Athletes presented increased sedentary time (pre-COVID = 7.0 ± 1.1 vs.COVID-19 = 8.9 ± 1.9 h/day, p = 0.004, d = 1.30) and significant decreases in the total PA observed in counts per day (pre-COVID = 2,967,064.4 ± 671,544.1 vs. COVID-19 = 1,868,210.2 ± 449,768.4 counts/day, p = 0.001, d = 1.99), time in vigorous PA (pre-COVID = 7.7 ± 0.9 vs. COVID-19 = 6.1 ± 1.2 h/day, p = 0.001, d = 1.56), and time in moderate-to-vigorous PA (pre-COVID = 8.1 ± 0.9 vs. COVID-19 = 6.5 ± 1.3 h/day, p = 0.001, d = 1.48). There were no significant differences for time in light and moderate PA or in sleep parameters (p > 0.05). Conclusion: Young badminton athletes presented increased sedentary time, and decreased total physical activity, time in MVPA, and time in vigorous activities during the COVID-19 pandemic compared to the pre-COVID period, however, there were no significant differences in sleep parameters. Immunometabolism of Skeletal Muscle and Exercise Research Group Department of Physical Education Federal University of Piauí (UFPI) Ministro Petrônio Portella” Campus Department of Psychiatry Post-Graduation Program in Health Sciences Santo Amaro University Sao Paulo Brazil and Bipolar Disorder Program (PROMAN) University of Sao Paulo Medical School Department of Physical Education São Paulo State University (UNESP) Presidente Prudente Department of Biophysics and Physiology Federal University of Piauí (UFPI), Campus Minister Petrônio Portela Department of Physical Education São Paulo State University (UNESP) Presidente Prudente

Background: Chlamydia abortus infections (Chlamydiosis) can cause reproductive problems in sheep, such as abortions and birth defects, leading to farm productivity loss. The symptoms, which are similar to other reproductive diseases, and the microbial pathogenesis make the clinical diagnosis difficult. Chlamydia abortus is a zoonotic pathogen, making it a public health issue because it can infect and induce abortions in humans. This study investigated anti-C. abortus antibody levels and infection risk factors in sheep in the State of Ceará, Brazil. Materials, Methods & Results: Forty-three properties from 10 municipalities in 4 mesoregions in the State of Ceará, Brazil (Sertões, metropolitan Fortaleza, North Ceará and Northwest Ceará) with sheep, goats, cattle, and horses were visited. Five hundred and four serological samples from sheep were collected and tested for anti-C. abortus antibodies using an Enzyme-linked Immunosorbent Assay (ELISA) [IDEXX®, Australia] and all procedures were performed in accordance with the manufacturer's instructions at the Clinical Pathology Laboratory of EMBRAPA Goats and Sheep (Sobral, Brazil). Individual questionnaires were completed about sheep breeding practices and to identify possible C. abortus risks. Seropositive results were found in 18.45 % (93/504 individuals) of sheep, and 88.37 % (38/43 properties) of the herds had at least one seropositive animal. The number of seropositive individuals was significantly different between adults and ewes [P < 0.01; Odds Ratio (OR) = 0.510; 95% confidence interval (CI) = 0.306 - 0.850]. Logistic regression modeling identified a missing health certificate request for newly acquired animals as a chlamydiosis risk factor [P = 0.038; OR = 2.672; 95% CI = 1.058 - 6.749]. Discussion: The prevalence of anti-C. abortus antibodies in sheep in the State of Ceara emphasizes the importance of testing and tracking the disease spread among herds; these results were similar to studies in other areas of Brazil. Adult sheep that spend more time on the property may have a higher exposure risk because of increased reproductive activity. Misinformation and technical limitations can influence the proper handling of animals avoiding contagion through the correct use of techniques and recommendations. Disease transmission occurs through the digestive tract and between mother and fetus. Therefore, seropositive (infected) sheep may be related to the breeding system practices, such as allowing contact between sheep and other species on the property (goats, cattle, and horses) during breeding. Acquiring animals from external sources without sufficient health information can increase the transmission risk. Contaminated pastures, water, food, and air also increase transmission risk. The lack of technical and practical knowledge regarding disease prevention and control also contributes to disease transmission, resulting in reproductive losses due to high abortion rates. Chlamydia abortus has zoonotic potential and may infect humans without proper safety information. Therefore, future epidemiological studies are required for a better understanding of the primary risk factors for disease occurrence and spread among herds in the region. Chlamydia abortus infection is present in sheep in Ceará, Brazil. Chlamydiosis information programs should be adopted, sanitary measures implemented, and the epidemiological surveillance of sheep herds strengthened. Made available in DSpace on 2021-12-10T12:05:08Z (GMT). No. of bitstreams: 1 CNPC-2021-Art-95.pdf: 180715 bytes, checksum: de689ae28b0c057f2f962d12569f525f (MD5) Previous issue date: 2021

Made available in DSpace on 2022-05-01T06:31:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The process of composting has been proposed as a biological alternative to improve the quality of tannery sludge (TS) by the action of microbial communities. However, there is limited knowledge about the dynamic of these microbial communities during the composting process. This study assessed the responses of bacterial and archaeal communities during TS composting using the 16S rRNA sequencing. The composting process occurred within 90 days, and samples of compost were collected on day 7 (d7; mesophilic stage), 30 (d30; thermophilic stage), 60 (d60; cooling stage), and 90 (d90; maturation stage). The results showed a succession of microbial phyla during the composting with enrichment of Synergistetes, WS1, and Euryarchaeota at the mesophilic stage, while at the thermophilic stage, there was an enrichment of Hydrogenedentes, WPS-2, Chloroflexi, and Deinococcus-Thermus. At the cooling stage, there was an enrichment of Kiritimatiellaeota, and at the maturation stage, there was an enrichment of Entotheonellaeota, Dadabacteria, Nitrospirae, Dependiatiae, and Fibrobacteres. When analyzing the drivers influencing microbial communities, Cr and pH presented more negative correlations with general phyla. In contrast, S, C, K, temperature, and N presented more positive correlations, while Ni, Cd, and P showed fewer correlations. According to niche occupancy, we observed a decreased proportion of generalists with a consequently increased proportion of specialists following the composting process. This study showed that different stages of the composting present a specific microbial community structure and dynamics, which are related to some specific composting characteristics. Soil Quality Lab. Agricultural Science Center Federal University of Piauí Soil Science Department Federal University of Ceará Technology Department São Paulo State University Graduate Program in Environmental Science Campus of Descalvado Brazil University University of West São Paulo Laboratory of Microbial Ecology and Biotechnology Federal University of Ceara Cellular and Molecular Laboratory Center for Nuclear Energy in Agriculture University of Sao Paulo Technology Department São Paulo State University

Background: To investigate the acute effects of a capsaicin analogue supplement on 10 km time-trial performance and physiological responses in amateur athletes. Methods: Twenty-one participants (age = 29.3 &plusmn, 5.5 years, weight 74.2 &plusmn, 11.3 kg, height 176.0 &plusmn, 0.0 cm, fat mass 12.7 &plusmn, 3.8%, V˙O2max 62.7 &plusmn, 8.4 mL&middot, k&minus, 1&middot, min&minus, 1), completed two randomized, double-blind trials: capsaicin analogue condition (Capsiate (CAP) = 24 mg) or a placebo (PLA) condition. The participants consumed two doses of 12 mg of CAP or PLA capsule 45 min before and immediately at the start of each trial. The time required to complete 10 km, lactate concentration, maximum heart rate (HRpeak), and rating of perceived exertion (RPE) were recorded. Results: The 10 km time-trial performance (CAP = 45.07 &plusmn, 6.41 min vs. PLA = 45.13 &plusmn, 6.73, p = 0.828) was not statistically significantly different between conditions. No statistically significant differences between conditions were detected for lactate concentration (p = 0.507), HRpeak (p = 0.897) and RPE (p = 0.517). Conclusion: Two doses of a 12 mg Capsaicin analogue supplement did not improve performance and physiological responses in a 10 km running time-trial in amateur athletes.

Made available in DSpace on 2021-06-25T10:14:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 Lira, FS, Conrado de Freitas, M, Gerosa-Neto, J, Cholewa, JM, and Rossi, FE. Comparison between full-body vs. split-body resistance exercise on the brain-derived neurotrophic factor immunometabolic response. J Strength Cond Res 34(11): 3094-3102, 2020-Intense aerobic exercise seems to increase serum concentrations of brain-derived neurotrophic factor (BDNF) in conjunction with increasing lactate; however, less is known about the BDNF response to differing resistance exercise protocols. We hypothesized that full-body (FB) resistance exercise will elicit a greater increase in serum BDNF and lactate compared with split-body resistance exercise. Twelve recreationally resistance-trained men (age = 25.3 ± 5.9 years) performed 3 randomized trials of 18 sets of exercise: upper-body (UB), lower-body (LB), and FB conditions. Serum BDNF levels were assessed at rest, immediately Post-exercise, Post-1 hour, and Post-2 hours during recovery. Lactate concentration was evaluated at rest, after 9 sets, Post-exercise, Post-5, Post-10, and Post-30 minutes during recovery. In addition, interleukin (IL-6 and IL-10) and the IL-6/IL-10 ratio were calculated. Lactate concentration and total volume were greater in the FB condition compared with LB and UB (p < 0.05). For BDNF, effect sizes were largest in the LB (1.4), followed by the FB (0.75), and moderate to UB (0.33), although no significant differences were observed between conditions. There was a statistically significant relationship between lactate and BDNF only for LB condition (rho = 0.72; p = 0.013). There were a greater IL-10 Post-1 hour for FB condition compared with UB and LB (p < 0.001), and lower IL-6/IL-10 ratio in FB compared with UB (p < 0.001). Lower body induced a great BDNF response, and FB resistance exercise elicited a greater increase of serum cytokines than UB in trained men. We speculate that the volume of work performed by larger muscles has a larger influence on BDNF than overall volume. Department of Physical Education Exercise and Immunometabolism Research Group São Paulo State University (UNESP) Department of Physical Education Skeletal Muscle Assessment Laboratory School of Technology and Sciences São Paulo State University Department of Kinesiology Coastal Carolina University Department of Physical Education Immunometabolism of Skeletal Muscle and Exercise Research Group Federal University of Piauí (UFPI) Department of Physical Education Exercise and Immunometabolism Research Group São Paulo State University (UNESP) Department of Physical Education Skeletal Muscle Assessment Laboratory School of Technology and Sciences São Paulo State University

The network of objects/things embedded with electronics, software, sensors, and network connectivity, Internet of Things (IoT), creates many exciting applications (e.g., smart grids, smart homes, and smart cities) by enabling objects/things to collect and exchange data so that they can be sensed and controlled. To fulfill IoT, one essential step is to connect various objects/things (e.g., mobile phones, cars, and buildings) so that they can “talk” to each other (i.e., collect and exchange data). However, substantial case studies show that simply connecting them without further collaboration among the objects/things when “talking” to each other leads to unnecessary energy consumption, uncertain security, unstable performance, etc., for IoT. Therefore, collaboration for IoT is very important. Specifically, there are a lot of critical issues to consider in terms of how to achieve robust collaboration among the objects/things for IoT. For instance, how to conduct collaboration among the objects/things so that more energy-efficient communication can be achieved for IoT? How to conduct collaboration among the objects/things so that computing with higher performance can be achieved for IoT? How to improve the security of IoT with collaboration among the objects/things? How to enhance the Quality of Service of IoT with collaboration among the objects/things? How to minimize the overhead costs when objects/things are collaborating in IoT?

International audience; In this paper, we extend the general descent method proposed by Attouch, Bolte and Svaiter [Math. Program. 137 (2013), 91-129] to deal with possible asymmetric like-distances. Using a w-distance as regularization term our results guarantee the convergence of bounded sequences, under the assumption that the objective function satisfies the Kurdyka-Łojasiewicz inequality. In particular, it improves some existing works on proximal point methods with quasi-distance as regularization term because we prove convergence of bounded sequences without any additional assumption on the w-distance unlike it have been done with quasi-distances. The last section gives an application relative to the emergence of habits after a succession of worthwhile moves which balance motivation and resistance to move.

Made available in DSpace on 2020-12-12T02:24:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-03 Background: Nutritional ergogenic aids are commonly used to boost physiological adaptations to exercise and promote greater fitness gains. However, there is a paucity of data about multi-ingredient pre-workout supplementation (MIPS). Therefore, the aim of the present study was to investigate the acute effects of MIPS on the oxidative, glycolytic and ATP-CP energy systems contribution, time spent above 90% V˙O2max (T90% V˙O2max), excess post-exercise oxygen consumption (EPOC) magnitude, number of efforts and time to exhaustion during a high-intensity interval exercise (HIIE) session. Methods: Twelve physically-active and healthy men completed the HIIE sessions that involved running bouts of 15 s on the treadmill at 120% of the maximum aerobic speed (MAS), interspersed with 15 s of passive recovery. Blood lactate was collected at immediately post, 3, 5, and 7 min post exercise. The contribution of ATP-CP, glycolytic and oxidative systems was analyzed at rest, during the HIIE sessions and for 20 min post. Performance variables (time to exhaustion, number of efforts) and oxygen consumption were also analyzed. Results: MIPS significantly increased the number of efforts performed (MIPS: 41 ± 10 vs Placebo: 36 ± 12, p = 0.0220) and time to exhaustion (MIPS: 20.1 ± 6 min vs Placebo: 17 ± 5 min, p = 0.0226). There was no difference between supplements for both T90% V˙O2max (p = 0.9705) and EPOC (p = 0.4930). Consuming MIPS significantly increased the absolute oxidative energy system contribution by 23.8% (p = 0.0163) and the absolute ATP-CP contribution by 28.4% (p = 0.0055) compared to placebo. There was only a non-significant tendency for a higher glycolytic system contribution after MIPS ingestion (p = 0.0683). Conclusion: Acute MIPS ingestion appears effective at increasing both aerobic and anaerobic alactic energy contribution and time to exhaustion during a HIIE protocol. Exercise and Immunometabolism Research Group Department of Physical Education School of Technology and Sciences Presidente Prudente Post Graduation Program in Physical Therapy São Paulo State University (UNESP) Immunometabolism of Skeletal Muscle and Exercise Research Group Department of Physical Education Associate Graduate Program in Health Science Federal University of Piauí (UFPI) Department of Kinesiology Laval University Laval VP Centro de Nutrição Funcional Post Graduation Program in Physical Therapy São Paulo State University (UNESP)

International audience; Diclofenac sodium (DS) is an emergent pollutant, and among the methods investigated for its removal, adsorption is the most widely utilized technique. Hydroxyapatite and chitosan are biomaterials often used for adsorption. However, both biomaterials are limited due to their low chemical stability in an acidic medium; furthermore, pure hydroxyapatite interacts poorly with diclofenac. In this work, hydroxyapatite was organofunctionalized with 3-aminopropyltrimethoxysilane and further used to obtain amino hydroxyapatite /chitosan hybrids by crosslinking with glutaraldehyde at pH 3, 4, 5, and 6. X-ray diffraction patterns indicated the preservation of the hydroxyapatite phase under all pH conditions. Based on the control reaction of the amino hydroxyapatite with glutaraldehyde and its further reduction in sodium borohydride, the formation of CN moieties was highlighted as the main interaction mechanism between the aldehyde and amino groups. Therefore, crosslinking with glutaraldehyde was evaluated by infrared, Raman spectroscopy, and 13C NMR techniques; the results suggested contributions of imine formation and hydrogen bonding. The hybrid obtained at pH 3 exhibited an enhanced adsorption capacity of 125 mg g−1 at 15 min. The synergy between amino hydroxyapatite and chitosan crosslinked by glutaraldehyde was demonstrated.

Made available in DSpace on 2022-04-30T20:25:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 The Family Ciconiidae currently comprises 19 species distributed primarily in the tropical and sub-tropical regions around the world. Recent studies on phylogeny within the genus and families of birds provided a new opportunity for the analysis of the cranial structure in a phylogenetical context. Consequently, the aim was to describe in detail the cranial osteology of the representative species of Ciconia (C. abdimii, C. ciconia, C. episcopus, C. maguari and C. nigra), aiming to compare the characters found amongst the species that form this genus and these with the one from other members of the Family Ciconiidae and determine the family relationships amongst the species, using the methodology of phylogenetic systematic. Cranial osteology has proven to be a good tool for the cladistic analysis of the genus Ciconia, providing osteologic characters that proves the monophyly of the group. The topology of the tree obtained in the present study has revealed itself as well resolved, presenting positive perspectives to the morphologic studies of the genus. The cladogram presented corroborates the hypothesis of the monophyletic characteristic of the Ciconia, the C. ciconia being the most basal of the genus. The topology of the cladogram is: (C. ciconia ((C. nigra (C. abdimii C. episcopus)) C. maguari)). In general, Ciconia presents a specialized skull, with peculiar characteristics and different from what is observed in other groups of birds. Federal University of Piauí Castanhal Faculty São Paulo State University São Paulo State University

Made available in DSpace on 2020-12-12T01:18:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 BACKGROUND: The purpose of this study was to investigate if a post-activation potentiation (PAP) protocol may attenuate the acute interference induced by high-intensity intermittent exercise (HIIE) and on subsequent strength exercise performance in recreationally trained men. METHODS: Eleven resistance-trained men (age: 25.7±3.7 y) randomly completed three experimental trials: strength exercise (SE) only (4 sets of maximal number of repetitions at 70% on the 45° leg press); concurrent exercise (CE) comprised 5000-m of HIIEat maximal aerobic speed (1:1 effort and pause ratio) followed by SEprotocol; CE with post-activation potentiation (CE-PAP), comprised the same CEprotocol preceded by one set of 2 repetitions at 90% of 1RM on the 45° leg-press before strength exercise. The number of repetitions performed was recorded for each set and total weight lifted was calculated. RESULTS: The CEcondition induced a greater decrement in volume for the leg press compared to SE and CE-PAP in sets 1 (24±21%; 18±25%), 2 (20±21%; 22±22%), and 3 (19±20%; 25±15%), respectively. Total weight lifted was greater after SE(8,795±2,581 kg) and CE-PAP(8.809±2.655 kg) conditions compared to CE(7.049±2.822 kg) (SEvs. CE: P=0.020) and (CE-PAP vs. CE: P=0.010) but there was no significant difference between SE and CE-PAP(P=1.00). CONCLUSIONS: PAP using a heavy load attenuated acute interference (total volume during lower-body strength) generated by HIIEprotocol, but did not enhance volume compared to SEalone. Skeletal Muscle Assessment Laboratory (LABSIM) Department of Physical Education School of Technology and Sciences São Paulo State University (UNESP) Department of Nutrition University of Western São Paulo (UNOESTE) School of Physical Education and Sport University of São Paulo Department of Kinesiology Coastal Carolina University Conway Immunometabolism of Skeletal Muscle and Exercise Research Group Federal University of Piauí (UFPI) Skeletal Muscle Assessment Laboratory (LABSIM) Department of Physical Education School of Technology and Sciences São Paulo State University (UNESP)

Made available in DSpace on 2022-04-28T19:47:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) This study was carried to evaluate the effects of tannins supplementation to sheep diets on their performance, carcass parameters and meat fatty acid profile through meta-analysis. Seventy-four peer-reviewed publications with 183 treatment means were included in the data set. The effects of tannins as an additive in sheep diets were evaluated by examining the weighted mean difference (WMD) between tannins treatment (diets with tannins inclusion) and control treatment (diets with no tannins). Heterogeneity was explored by meta-regression and subgroup analysis performed for: genetic group; animal age; tannins supplementation period, experimental design; amount of condensed tannins and concentrate in diet. Based on our results, tannins should not be fed to lamb younger than 3 months of age due to their negative effect on carcass weight. Enteric methane emissions (CH4 g/kg DMI) reduced by 17.5 % with tannins supplementation. Tannins supplementation also altered N metabolism by increasing N intake (2.10 %), retained N (5.44 %) and fecal N excretion (2.99 %) and reducing urinary N excretion (9.7 %). Nevertheless, the effects of tannins feeding on N metabolism varied according to the experimental design used (rotative vs. continuos). Therefore, the use of rotative experimental design in studies carried to evaluate the response of sheep to tannins can result in responses with confounded error due to the possibility of having residual effects of treatments. Increased meat concentrations of fatty acid with human health benefits were reported with tannins supplementation. Concentrations of eicosapentaenoic acid (EPA C20:5 ω-3; 26.63 %), docosapentaenoic (DPA C22:5 ω-3; 12.08 %), docosahexaenoic (DHA C22:6 ω-3; 10.59 %) and total omega-3 (14.01 %) were all higher in meat from sheep fed tannins compared to those fed control diet (diets with no tannins). Additionally, condensed tannins inclusion to sheep diets in amounts ranging from 20.0 to 25.0 g/kg of DM increased meat concentrations of arachidonic acid, EPA C20:5 ω-3, DPA C22:5 ω-3, DHA C22:6 ω-3, total PUFA, total omega-3 and total omega-6. Animal Unit of Digestive and Metabolic Studies in the Department of Animal Science School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP) Department of Agriculture Nutrition and Food Systems University of New Hampshire Federal University of Piauí Department of Biological Safety German Federal Institute for Risk Assessment Department of Animal Science Federal University of Espírito Santo School of Veterinary Medicine and Animal Science São Paulo State University School of Agricultural and Veterinarian Science São Paulo State University Animal Unit of Digestive and Metabolic Studies in the Department of Animal Science School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP) School of Veterinary Medicine and Animal Science São Paulo State University School of Agricultural and Veterinarian Science São Paulo State University

Made available in DSpace on 2022-05-01T04:26:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Soil microbial communities act on important environmental processes, being sensitive to the application of wastes, mainly those potential contaminants, such as tannery sludge. Due to the microbiome complexity, graph-theoretical approaches have been applied to represent model microbial communities interactions and identify important taxa, mainly in contaminated soils. Herein, we performed network and statistical analyses into microbial 16S rRNA gene sequencing data from soil samples with the application of different levels of composted tannery sludge (CTS) to assess the most connected nodes and the nodes that act as bridges to identify key microbes within each community. The network analysis revealed hubs belonging to Proteobacteria in soil with lower CTS rates, while active degraders of recalcitrant and pollutant chemical hubs belonging to Proteobacteria and Actinobacteria were found in soils under the highest CTS rates. The majority of classified connectors belonged to Actinobacteria, but similarly to hubs taxa, they shifted from metabolic functional profile to taxa with abilities to degrade toxic compounds, revealing a soil perturbation with the CTS application on community organization, which also impacted the community modularity. Members of Actinobacteria and Acidobacteria were identified as both hub and connector suggesting their role as keystone groups. Thus, these results offered us interesting insights about crucial taxa, their response to environmental alterations, and possible implications for the ecosystem. Institute of Science and Technology Universidade Federal de São Paulo Department of Agricultural Engineering and Soil Science Federal University of Piauí Laboratório de Ecologia Microbiana e Biotecnologia Federal University of Ceara Center for Nuclear Energy in Agriculture University of Sao Paulo University of Sao Paulo West, Campus II Universidade Estadual Paulista, Campus de Jaboticabal Universidade Brasil Universidade Estadual Paulista, Campus de Jaboticabal CNPq: 305069/2018-1 CNPq: 305102/2014-1 CNPq: 471347/2013-2

International audience; There are users and organizations that resist adopting cloud computing solutions, due to concerns about the security and privacy of their data. A Service Level Agreement (SLA) can be used to address these concerns, increasing trust in the purchased services through the clear description of the guarantees offered by the provider to the subscribers. For this purpose, the authors performed a literature systematic mapping to enumerate existing solutions and open issues in security SLAs in cloud computing. This review is presented in this paper as well as an analysis of the state of art. This paper also presents a classification of the selected papers and a discussion about management of security SLAs in clouds.

International audience; An idiopathic myopathy characterized by central nuclei in muscle fibers, a hallmark of muscle regeneration, has been observed in cancer patients. In cancer cachexia skeletal muscle is incapable of regeneration, consequently, this observation remains unaccounted for. In C26-tumor bearing, cachectic mice, we observed muscle fibers with central nuclei in the absence of molecular markers of bona fide regeneration. These clustered, non-peripheral nuclei were present in NCAM-expressing muscle fibers. Since NCAM expression is upregulated in denervated myofibers, we searched for additional makers of denervation, including AchRs, MUSK, and HDAC. This last one being also consistently upregulated in cachectic muscles, correlated with an increase of central myonuclei. This held true in the musculature of patients suffering from gastrointestinal cancer, where a progressive increase in the number of central myonuclei was observed in weight stable and in cachectic patients, compared to healthy subjects. Based on all of the above, the presence of central myonuclei in cancer patients and animal models of cachexia is consistent with motor neuron loss or NMJ perturbation and could underlie a previously neglected phenomenon of denervation, rather than representing myofiber damage and regeneration in cachexia. Similarly to aging, denervation-dependent myofiber atrophy could contribute to muscle wasting in cancer cachexia.

International audience; The present work describes the synthesis and characterization of hybrid materials based on β-cyclodextrin (β-CD) and cetyltrimethylammonium bromide (CTAB) intercalated into saponite (SAP) and a commercially available powder of anthocyanin dye, Crystal Red Grape (RG). The interactions between the organic dye guest and the organo-clay host were investigated by X-ray diffraction, thermogravimetric analysis, transmission electronic microscopy and 13C solid state nuclear magnetic resonance. The results support complex formation between β-CD and CTAB, their intercalation into the clay interlayer spaces or surface loading, and their interaction with RG (CTAB_SAP-RG, β-CD_SAP-RG and β-CD + CTAB_SAP-RG). The hybrid pigments formed exhibit different colors, enhanced stability against visible light irradiation and basic pH conditions. These hybrid pigments are environmentally friendly and can be promising candidates in different application fields.

International audience; Hybrid pigments based on organo-saponite (cetyltrimethyl ammonium bromide (CTAB-Sap) and a commercial anthocyanin (ACN) dye, Crystal Red Grape, were prepared. The interactions between organic dye guest and hosts (including saponite and organo-saponite) were investigated by X-ray diffraction, transmission electron microscopy and Fourier transform infrared spectroscopy. The pigments exhibit different colors function of their host-guest interactions. The blue color of organo-clay-anthocyanin indicates the stabilization of quinoidal base form of the dye. The hybrids have good stability against visible light irradiation and basic pH conditions. These dyed materials are environmentally friendly and can be promising candidates in different application fields.

Made available in DSpace on 2022-04-29T08:45:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 To evaluate the effect of accelerated artificial aging (AAA) on chemical and physical properties of four different resin cements and two flowable resins. Forty-eight human incisors were prepared and the IPS Empress Esthetic laminate veneers were fabricated. The samples were randomly divided into the groups: dual-polymerizing cements (RelyX ARC and Variolink II), light-polymerizing cements (RelyX Veneer and Variolink Veneer) or flowable composite resins (Filtek Z350 Flow and Tetric N-Flow). The degree of conversion was determined in a Fourier transform infrared spectroscopy. The first cylinder was measuring the initial bond strength and, the remaining cylinder was submitted to AAA before bond strength test. The fracture pattern was analyzed by stereomicroscopy. The results were analyzed by one-way and two-way split-spot ANOVA/ Tukey's test (α =0.05). There were clinically perceptible color changes for all materials. The degree of conversion, the following decreasing sequence was found: RelyX ARC> Variolink II> RelyX Veneer > Filtek Z350 Flow > Variolink Veneer = Tetric N-Flow. For the microshear bond strength, RelyX ARC = Variolink II > Variolink Veneer = RelyX Veneer > flowable composite resins. AAA induced color change and decreased microshear bond strength for all luting systems evaluated. Department of Restorative Dentistry Piracicaba Dental School State University of Campinas Department of Dentistry Faculty of Sciences of Health Federal University of Jequitinhonha and Mucuri Valley Department of Biological Sciences Bauru School of Dentistry University of São Paulo Department of Dental Materials and Prosthesis Paulista State University Alberto Luiz Coimbra Institute for Graduate Studies and Engineering Research Federal University of Rio de Janeiro Department of Dental Clinic Federal University of Piauí Department of Pediatric Dentistry and Orthodontics Federal University of Rio de Janeiro Department of Dental Clinic Federal University of Rio de Janeiro Department of Dental Materials and Prosthesis Paulista State University

Made available in DSpace on 2020-12-12T01:34:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Objectives: Performance in running-based sport depends on the ability to perform repetitive high intensity muscle contractions. Previous studies have shown that capsaicin analog (CAP) (i.e. Capsiate) supplementation may improve this performance. The purpose of this study was to investigate the acute effect of CAP supplementation on short (400 m) and middle distance (3000 m) running time-trial performance, maximum heart rate (HR), and rate of perceived exertion (RPE). Methods: Twelve physically active men completed four randomized, double-blind trials: CAP condition (12 mg) or a placebo condition. Forty-five minutes after supplementation, the participants performed a 400-or 3000-meter running time trial. Time (in seconds) was recorded. HR was analyzed at rest and immediately post-exercise, and RPE was collected immediately after exercise. Results: For both the 400 m time-trial (CAP = 66.4 + 4.2 sec vs Placebo = 67.1 + 4.8 sec, p = 0.046) and the 3000 m time-trial (CAP = 893.9 ± 46.8 sec vs Placebo = 915.2 ± 67.6 sec, p = 0.015), the time in seconds was significantly less in the CAP compared to placebo conditions. There were no statistically significant differences for HR and RPE in any condition. Conclusion: In summary, acute CAP supplementation improved 400 m and 3000 m running time-trial performance in a distance-dependent way but without modifying the HR and RPE. Department of Physical Education Federal University of Piauí (UFPI) Department of Nutrition University of Western São Paulo (UNOESTE) Department of Kinesiology Coastal Carolina University Department of Sport School of Physical Education and Sport University of São Paulo UPE/UFPB Department of Physical Education São Paulo State University (UNESP) Department of Biophysics and Physiology Federal University of Piaui, Campus Minister Petrônio Portela, Ininga Department of Physical Education Federal University of Maranhão Federal University of Piauí (UFPI) Department of Physical Education São Paulo State University (UNESP)

International audience; The polycondensation of amino acids to oligopeptides is an important step in the origins of life, and known to be effective on several mineral surfaces. The data available on clay mineral surfaces are heterogeneous and sometimes contradictory, however. The objective of the present work is to investigate the adsorption of a selected amino acid, alanine, in expanding and non-expanding clays and then study the possible peptide condensation by thermal activation. A multi-technique approach was used, including macroscopic measurements of the adsorption process (adsorption isotherms and pH-metry), and in situ molecular-level characterization of the solids obtained (X-ray diffraction, thermogravimetric analysis and infrared spectroscopy). The results indicate that only weak interaction is established between alanine molecule and kaolinite surfaces, most of the deposited molecules being only physically retained. Thermal activation of alanine/kaolinite only led to desorption. In contrast, higher-energy adsorption mechanisms were at play in hectorite, including cation exchange and coordinative adsorption to the interlayer ions, and alanine species adsorbed in this way were observed to form cyclic dimers upon activation between 160 and 270 °C.

International audience; In this work, hectorite/alizarin pigments were prepared at pH 9 and 12. Hectorite was initially modified with cetyltrimethylammonium and pectine to produce organo-hectorites. The influence of organo-hectorite on the stabilization of alizarin was evaluated. X-ray diffraction suggested the formation of exfoliated/delaminated structures after organofunctionalization of the hectorite. For raw hectorite and their derivatives with pectin, the formation of hybrid pigments was confirmed by infrared spectroscopy. Thermogravimetry curves confirm the hectorite organofunctionalization and that the alizarin loading was higher at pH 12. The hybrids prepared at pH 12 show more photostability than ones prepared at pH 9. This behavior was probably due to the total deprotonation of dye molecules, which promoted better interactions between the hosts and the guests.