Search

Your search keyword '"Clara Sanchez"' showing total 116 results
Start Over Author "Clara Sanchez"
116 results on '"Clara Sanchez"'

1. Defects in AMPAR trafficking and microglia activation underlie socio-cognitive deficits associated to decreased expression of phosphodiesterase 2 a

Author

Sébastien Delhaye, Marielle Jarjat, Asma Boulksibat, Clara Sanchez, Alessandra Tempio, Andrei Turtoi, Mauro Giorgi, Sandra Lacas-Gervais, Gabriele Baj, Carole Rovere, Viviana Trezza, Manuela Pellegrini, Thomas Maurin, Enzo Lalli, and Barbara Bardoni

Subjects
cAMP and cGMP pathways, PDE2A, Autism Spectrum disorder, Intellectual disability, Mouse model, mGluR-dependent LTD, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Phosphodiesterase 2 A (PDE2A) is an enzyme involved in the homeostasis of cAMP and cGMP and is the most highly expressed PDE in human brain regions critical for socio-cognitive behavior. In cerebral cortex and hippocampus, PDE2A expression level is upregulated in Fmr1-KO mice, a model of the Fragile X Syndrome (FXS), the most common form of inherited intellectual disability (ID) and autism spectrum disorder (ASD). Indeed, PDE2A translation is negatively modulated by FMRP, whose functional absence causes FXS. While the pharmacological inhibition of PDE2A has been associated to its pro-cognitive role in normal animals and in models of ID and ASD, homozygous PDE2A mutations have been identified in patients affected by ID, ASD and epilepsy. To clarify this apparent paradox about the role of PDE2A in brain development, we characterized here Pde2a+/− mice (homozygote animals being not viable) at the behavioral, cellular, molecular and electrophysiological levels. Pde2a+/− females display a milder form of the disorder with reduced cognitive performance in adulthood, conversely males show severe socio-cognitive deficits throughout their life. In males, these phenotypes are associated with microglia activation, elevated glutathione levels and increased externalization of Glutamate receptor (GluR1) in CA1, producing reduced mGluR-dependent Long-term Depression. Overall, our results reveal molecular targets of the PDE2A-dependent pathway underlying socio-cognitive performance. These results clarify the mechanism of action of pro-cognitive drugs based on PDE2A inactivation, which have been shown to be promising therapeutic approaches for Alzheimer's disease, schizophrenia, FXS as well as other forms of ASD.

Published
2024
Full Text
View/download PDF

2. Soil-borne fungi alter the apoplastic purinergic signaling in plants by deregulating the homeostasis of extracellular ATP and its metabolite adenosine

Author

Christopher Kesten, Valentin Leitner, Susanne Dora, James W Sims, Julian Dindas, Cyril Zipfel, Consuelo M De Moraes, and Clara Sanchez-Rodriguez

Subjects
Fusarium oxysporum, Adenosine, apoplast, Medicine, Science, Biology (General), QH301-705.5
Abstract

Purinergic signaling activated by extracellular nucleotides and their derivative nucleosides trigger sophisticated signaling networks. The outcome of these pathways determine the capacity of the organism to survive under challenging conditions. Both extracellular ATP (eATP) and Adenosine (eAdo) act as primary messengers in mammals, essential for immunosuppressive responses. Despite the clear role of eATP as a plant damage-associated molecular pattern, the function of its nucleoside, eAdo, and of the eAdo/eATP balance in plant stress response remain to be fully elucidated. This is particularly relevant in the context of plant-microbe interaction, where the intruder manipulates the extracellular matrix. Here, we identify Ado as a main molecule secreted by the vascular fungus Fusarium oxysporum. We show that eAdo modulates the plant’s susceptibility to fungal colonization by altering the eATP-mediated apoplastic pH homeostasis, an essential physiological player during the infection of this pathogen. Our work indicates that plant pathogens actively imbalance the apoplastic eAdo/eATP levels as a virulence mechanism.

Published
2023
Full Text
View/download PDF

4. The companion of cellulose synthase 1 confers salt tolerance through a Tau-like mechanism in plants

Author

Christopher Kesten, Arndt Wallmann, René Schneider, Heather E. McFarlane, Anne Diehl, Ghazanfar Abbas Khan, Barth-Jan van Rossum, Edwin R. Lampugnani, Witold G. Szymanski, Nils Cremer, Peter Schmieder, Kristina L. Ford, Florian Seiter, Joshua L. Heazlewood, Clara Sanchez-Rodriguez, Hartmut Oschkinat, and Staffan Persson

Subjects
Science
Abstract

The Arabidopsis CC1 protein maintains microtubule array stability and cellulose synthesis during salt stress. Here the authors show that CC1 engages microtubules via an intrinsically disordered N-terminus that suggests it controls microtubule dynamics in a similar way to the mammalian Tau protein.

Published
2019
Full Text
View/download PDF

5. Estabilidad y nivel óseo periimplantario de implantes postextractivos en pacientes de la tercera edad

Author

Orlando Guerra Cobian, Clara Sanchez Silot, and Jorge Luis García Romero

Subjects
implantes postextractivos, estabilidad, pérdida ósea periimplantaria, adulto mayor., Medicine (General), R5-920, Public aspects of medicine, RA1-1270
Abstract

Introducción: Los implantes postextractivos acortan el tiempo en lograr la rehabilitación del paciente, resulta esta condicionante un factor esencial para devolver la calidad de vida en corto plazo a un adulto mayor y mejorar rápidamente su función masticatoria. Objetivo: Determinar los valores de estabilidad y la pérdida ósea periimplantaria en implantes postextractivos en pacientes de la tercera edad. Material y Método: Se realizó un estudio cuasi-experimental en 99 pacientes de la tercera edad en la Facultad de Estomatología ¨Raúl González Sánchez¨, 2017-2019. Bajo su consentimiento se colocaron 173 implantes postextractivos. Se determinó tipo de hueso de soporte, estabilidad primaria y secundaria según análisis de frecuencia de resonancia con Osstel Mentor. Se midió el nivel óseo periimplantario y la pérdida ósea hasta 12 meses de colocada la rehabilitación. Resultados: Se posicionaron mayoritariamente implantes en el sitio de implantación incisivo maxilar en 43,3 % de los casos. Los valores promedio de estabilidad primaria y secundaria fueron 48 ISQ y 68 ISQ respectivamente. La pérdida ósea promedio tras un año de rehabilitación fue de 1,04±0,22mm. Conclusiones: Los implantes dentales postextractivos en pacientes de la tercera edad se insertaron preferentemente en el grupo incisivo maxilar y en hueso tipo D2, registraron una estabilidad primaria promedio moderada y una estabilidad secundaria promedio substancial. La pérdida ósea vertical periimplantaria exhibió valores semejantes a los implantes en zonas curadas y dentro del valor estandarizado para pérdida ósea periimplantaria para el primer año tras su colocación.

Published
2020

6. The Neuropeptide Cortistatin Alleviates Neuropathic Pain in Experimental Models of Peripheral Nerve Injury

Author

Clara P. Falo, Raquel Benitez, Marta Caro, Maria Morell, Irene Forte-Lago, Pedro Hernandez-Cortes, Clara Sanchez-Gonzalez, Francisco O’Valle, Mario Delgado, and Elena Gonzalez-Rey

Subjects
neuropathic pain, neuropeptide, neuroprotection, neuroregeneration, peripheral nerve injury, neuroinflammation, Pharmacy and materia medica, RS1-441
Abstract

Neuropathic pain is one of the most severe forms of chronic pain caused by the direct injury of the somatosensory system. The current drugs for treating neuropathies have limited efficacies or show important side effects, and the development of analgesics with novel modes of action is critical. The identification of endogenous anti-nociceptive factors has emerged as an attractive strategy for designing new pharmacological approaches to treat neuropathic pain. Cortistatin is a neuropeptide with potent anti-inflammatory activity, recently identified as a natural analgesic peptide in several models of pain evoked by inflammatory conditions. Here, we investigated the potential analgesic effect of cortistatin in neuropathic pain using a variety of experimental models of peripheral nerve injury caused by chronic constriction or partial transection of the sciatic nerve or by diabetic neuropathy. We found that the peripheral and central injection of cortistatin ameliorated hyperalgesia and allodynia, two of the dominant clinical manifestations of chronic neuropathic pain. Cortistatin-induced analgesia was multitargeted, as it regulated the nerve damage-induced hypersensitization of primary nociceptors, inhibited neuroinflammatory responses, and enhanced the production of neurotrophic factors both at the peripheral and central levels. We also demonstrated the neuroregenerative/protective capacity of cortistatin in a model of severe peripheral nerve transection. Interestingly, the nociceptive system responded to nerve injury by secreting cortistatin, and a deficiency in cortistatin exacerbated the neuropathic pain responses and peripheral nerve dysfunction. Therefore, cortistatin-based therapies emerge as attractive alternatives for treating chronic neuropathic pain of different etiologies.

Published
2021
Full Text
View/download PDF

7. Unmarried Mothers on the English Stage after the Godly Revolution/Madres solteras en la escena inglesa tras la Revolucion gloriosa

Author

Trigo, Clara Sanchez

Subjects
The Marriage-Hater Matched (Play) -- Criticism and interpretation, The Squire of Alsatia (Play) -- Criticism and interpretation, Dramatists -- Criticism and interpretation, Unmarried mothers -- Portrayals, Languages and linguistics, Literature/writing, Criticism and interpretation, Portrayals
Abstract

Unmarried mothers and children born out of wedlock are notoriously absent from the Carolean stage, which, on the other hand, saw an abundance of rakish gallants and prostitutes populating sexually charged plots. It is not a coincidence that the only two plays premiered between 1660 and 1700 featuring an unmarried woman with a living child arrive just around the time that William and Mary take the throne. This paper analyses the ways in which these two plays--The Squire of Alsatia (1688) by Thomas Shadwell and The Marriage-Hater Matched (1692) by Thomas Durfey--use the unmarried mother to convey a moral message that echoed William and Mary's project of moral reform. However, these women are portrayed in radically different ways in the two comedies. Although she is customarily portrayed as a despicable character in The Squire of Alsatia, the unmarried mother in The Marriage-Hater Matched gets to be a good-hearted heroine who is eventually redeemed. This was a very challenging position for Durfey to portray, requiring of intra- and extra textual devices to support her redemption without appearing to condone vice. Keywords: Restoration comedy; mothers; moral reform; Thomas Durfey; Thomas Shadwell Las madres solteras y los ninos nacidos fuera del matrimonio estan significativamente ausentes de la escena carolina, en la cual, por otra parte, existia un abundante numero de galantes libertinos y prostitutas que formaban parte de argumentos cargados de sexualidad. No es casualidad que las dos unicas obras estrenadas entre 1660 y 1700 con una madre soltera entre sus personajes se vean justo en la epoca en que Guillermo y Maria suben al trono. Este articulo analiza las formas en que estas dos obras--The Squire of Alsatia (1688), de Thomas Shadwell y The Marriage-Hater Matched (1692), de Thomas Durfey--utilizan a la madre soltera para transmitir un mensaje moral que se hace eco del proyecto de reforma de indole moral de Guillermo y Maria. Sin embargo, estas mujeres son descritas de maneras radicalmente diferentes en las dos comedias. Mientras que en The Squire of Alsatia se representa a la madre soltera como un personaje despreciable, en The Marriage-Hater Matched esta se convierte en una heroina de buen corazon que finalmente es redimida, una posicion muy dificil de retratar para Durfey, que requirio de recursos intratextuales y extratextuales para transmitir la idea de redencion sin que pareciera que se admitia el vicio. Palabras clave: Comedia de la Restauracion; madres; reforma moral; Thomas Durfey; Thomas Shadwell, Carolean comedy revelled in complex plots where prostitutes, hypocritical Puritans, rascals and bullies rubbed shoulders with ladies and gentlemen of quality. Debauchery and licentiousness were necessary attitudes for comic gentlemen [...]

Published
2024
Full Text
View/download PDF

8. Guidelines for naming and studying plasma membrane domains in plants

Author

Jaillais, Yvon, Bayer, Emmanuelle, Bergmann, Dominique C., Botella, Miguel A., Boutté, Yohann, Bozkurt, Tolga O., Caillaud, Marie-Cecile, Germain, Véronique, Grossmann, Guido, Heilmann, Ingo, Hemsley, Piers A., Kirchhelle, Charlotte, Martinière, Alexandre, Miao, Yansong, Mongrand, Sebastien, Müller, Sabine, Noack, Lise C., Oda, Yoshihisa, Ott, Thomas, Pan, Xue, Pleskot, Roman, Potocky, Martin, Robert, Stéphanie, Rodriguez, Clara Sanchez, Simon-Plas, Françoise, Russinova, Eugenia, Van Damme, Daniel, Van Norman, Jaimie M., Weijers, Dolf, Yalovsky, Shaul, Yang, Zhenbiao, Zelazny, Enric, and Gronnier, Julien

Published
2024
Full Text
View/download PDF

10. Neurodevelopmental and growth outcomes after invasive Group B Streptococcus in early infancy: A multi-country matched cohort study in South Africa, Mozambique, India, Kenya, and Argentina

Author

Madhi, Shabir A., Dangor, Ziyaad, Leahy, Shannon, Harden, Lois, Ghoor, Azra, Mbatha, Sibongile, Lowick, Sarah, Laughton, Barbara, Jaye, Tamara, Lala, Sanjay G, Sithole, Pamela, Msayi, Jacqueline, Kumalo, Ntombifuthi, Msibi, Tshepiso Nompumelelo, Santhanam, Sridhar, John, Hima B., Arumugam, Asha, Murugesan, Nandhini, Rajendraprasad, Nandhini, Priya, Mohana, Abubakar, Amina, Nasambu, Carophine, Adan, Adam Mabrouk, Katana, Patrick Vidzo, Mwangome, Eva, Newton, Charles R., Bassat, Quique, Bardají, Azucena, Bramugy, Justina, Mucasse, Humberto, Aerts, Celine, Massora, Sergio, Libster, Romina, Yanotti, Clara Sánchez, Medina, Valeria, Rojas, Andrea, Amado, Daniel, Llapur, Conrado J., Hossain, A.K.M. Tanvir, Sadeq-ur Rahman, Qazi, Paul, Proma, Chandna, Jaya, Procter, Simon R., Seedat, Farah, Horváth-Puhó, Erzsébet, Jit, Mark, Milner, Kate, Gonçalves, Bronner P., and Lawn, Joy E.

Published
2022
Full Text
View/download PDF

15. Tu sei parte di me

Author

Federica Bosco, Cristina Caboni, Valentina D'Urbano, Evita Greco, Clara Sanchez, Carmela Scotti, Simona Sparaco

Published
2018

17. Evidence for Constitutive Microbiota-Dependent Short-Term Control of Food Intake in Mice: Is There a Link with Inflammation, Oxidative Stress, Endotoxemia, and GLP-1?

Author

Selma Ben Fradj, Emmanuelle Nédélec, Juliette Salvi, Mélanie Fouesnard, Marine Huillet, Gaëtan Pallot, Céline Cansell, Clara Sanchez, Catherine Philippe, Vincent Gigot, Aleth Lemoine, Doriane Trompier, Thomas Henry, Virginie Petrilli, Benedicte F. Py, Hervé Guillou, Nicolas Loiseau, Sandrine Ellero-Simatos, Jean-Louis Nahon, Carole Rovère, Jacques Grober, Gaelle Boudry, Véronique Douard, Alexandre Benani, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Agro Dijon, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Toxicologie Intégrative & Métabolisme (ToxAlim-TIM), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the French ‘‘Investissements d’Avenir’’ program, project ISITE-BFC (contract National Research Agency (ANR)-15-IDEX-0003), by the ANR (contract ANR-21-CE14-0033), by the Re´gion Bourgogne and FEDER program (contract 2019-6200FEO047S00409) toA.B., by the INRAE grant ‘‘Action Prioritaire du De´partement Alimentation Humaine’’ to A.B., G.B., and V.D., by the Medisite Foundation and the French government, managed by the ANR, through the UCAJEDI Investments in the Future project (contract ANR-15-IDEX-01) to C.C., C.S.,J.L.N., and C.R., by the LABEX SIGNALIFE program (contract ANR-11-LABX-0028-01) to J.L.N. and C.R., and by the Fondation pour la Recherche Me´dicale (contract DEQ20170336744) to V.P., ANR-15-IDEX-0003,BFC,ISITE ' BFC(2015), ANR-21-CE14-0033,MicroFlamEAT,Rôle de la réponse inflammatoire microgliale postprandiale dans le contrôle cérébral du comportement alimentaire(2021), ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Physiologie de la Nutrition et du Comportement Alimentaire (PNCA (UMR 0914)), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Département cancer environnement (Centre Léon Bérard - Lyon), Centre Léon Bérard [Lyon], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Institut Agro Dijon, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
food intake, Physiology, [SDV]Life Sciences [q-bio], satiety, Clinical Biochemistry, eating disorders, digestive system, Biochemistry, Eating, Mice, Glucagon-Like Peptide 1, Mice, Inbred NOD, Appetite Depressants, microbiota, Animals, energy homeostasis, Molecular Biology, General Environmental Science, Inflammation, digestive, oral, and skin physiology, Cell Biology, Endotoxemia, Oxidative Stress, gut, General Earth and Planetary Sciences, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; AIMS: Although prebiotics, probiotics, and fecal transplantation can alter the sensation of hunger and/or feeding behavior, the role of the constitutive gut microbiota in the short-term regulation of food intake during normal physiology is still unclear. RESULTS: An antibiotic-induced microbiota depletion study was designed to compare feeding behavior in conventional and microbiota-depleted mice. Tissues were sampled to characterize the time profile of microbiota-derived signals in mice during consumption of either standard or high-fat food for 1 hour. Pharmacological and genetic tools were used to evaluate the contribution of postprandial endotoxemia and inflammatory responses in the short-term regulation of food intake. We observed constitutive microbial and macronutrient-dependent control of food intake at the time scale of a meal, i.e., within 1 hour of food introduction. Specifically, microbiota depletion increased food intake and the microbiota-derived anorectic effect became significant during the consumption of high-fat but not standard food. This anorectic effect correlated with a specific postprandial microbial metabolic signature and did not require postprandial endotoxemia or an NLRP3 (NOD-, LRR- and Pyrin domain-containing protein 3)-inflammasome mediated inflammatory response. Innovation and Conclusion: These findings show that the gut microbiota controls host appetite at the time scale of a meal under normal physiology. Interestingly, a microbiota-derived anorectic effect develops specifically with a high-fat meal, indicating that gut microbiota activity is involved in the satietogenic properties of foods.

Published
2022
Full Text
View/download PDF

22. DIAGNÓSTICO DAS LEIS ESTADUAIS DE INCENTIVO AO ESPORTE NO BRASIL

Author

Sabrina de Lima Vitório, Leandro Eduardo Cerbi, Bruna Lindman Bueno, Clara Sanchez da Trindade Santos, and Leandro Carlos Mazzei

Subjects
General Medicine
Abstract

Com a criação da Lei Federal de Incentivo ao Esporte em 2006 e na onda dos Megaeventos Esportivos no Brasil, muitos Estados criaram suas próprias iniciativas relacionadas à isenção fiscal para favorecimento do Esporte. No entanto, a bibliografia sobre este fenômeno é escassa. Assim, este estudo teve por objetivo apresentar um diagnóstico das Leis de Incentivo ao Esporte (LIEs) no âmbito Estadual, indicando as características destas ações realizadas pelas unidades federativas. Trata-se de um estudo de caráter descritivo, utilizando de fontes documentais para a coleta de dados obtidas através dos sites oficiais de cada Secretaria ou órgão semelhante responsável pelas Políticas Públicas do Esporte nos Estados. Foram identificadas ações relacionadas ao incentivo do esporte via isenção fiscal em 15 Estados e todas envolvem o tributo ICMS. Verificou-se ainda uma variação grande com relação às porcentagens de valor destinadas, bem como uma diversificação das manifestações esportivas em cada Estado.

Published
2021
Full Text
View/download PDF

26. Wafer‐scale Ge epitaxial foils grown at high growth rates and released from porous substrates for triple‐junction solar cells

Author

Valérie Depauw, Clément Porret, Myriam Moelants, Emma Vecchio, Koen Kennes, Han Han, Roger Loo, Jinyoun Cho, Guillaume Courtois, Rufi Kurstjens, Kristof Dessein, Víctor Orejuela, Clara Sanchez‐Perez, Ignacio Rey‐Stolle, and Iván García

Subjects
Renewable Energy, Sustainability and the Environment, Electrical and Electronic Engineering, Condensed Matter Physics, Electronic, Optical and Magnetic Materials
Published
2022
Full Text
View/download PDF

27. Predictors of cardiovascular events and all‐cause of death in patients with transfusion‐dependent myelodysplastic syndrome

Author

Tamara Jimenez-Solas, Agustín C. Martín-García, Teresa Gonzalez-Martinez, Elena Díaz-Peláez, Marta Alonso-Fernández-Gatta, Ana Martín-García, Félix López-Cadenas, Pedro L. Sánchez, María Díez-Campelo, Clara Sanchez-Pablo, Junta de Castilla y León, and Instituto de Salud Carlos III

Subjects
Male, Risk, medicine.medical_specialty, Iron Overload, Multivariate analysis, Heart disease, Population, Kaplan-Meier Estimate, Disease, 03 medical and health sciences, 0302 clinical medicine, Cause of Death, Internal medicine, Humans, Medicine, Blood Transfusion, Prospective Studies, education, Prospective cohort study, Pathological, Aged, Cause of death, Aged, 80 and over, education.field_of_study, business.industry, Hematology, Middle Aged, T1 mapping, Prognosis, Cardiovascular disease, medicine.disease, Magnetic Resonance Imaging, Log-rank test, Feature tracking, Cardiovascular Diseases, NT-proBNP, Myelodysplastic Syndromes, 030220 oncology & carcinogenesis, Cardiology, Female, business, Myelodysplastic syndrome, Biomarkers, Follow-Up Studies, 030215 immunology
Abstract

Cardiovascular disease (CVD) involves the second cause of death in low-risk myelodysplastic syndrome (MDS) population. Prospective study to characterise the CVD and to identify predictors for the combined event (CE) cardiovascular event and/or all-cause mortality in transfusion dependent low-risk MDS patients. Thirty-one patients underwent a cardiac assessment including biomarkers and cardiac magnetic resonance (cMR) with parametric sequences (T1, T2 and T2* mapping) and myocardial deformation by feature tracking (FT) and were analysed for clonal hematopoiesis of indeterminate potential mutations. Cardiac assessment revealed high prevalence of unknown structural heart disease (51% cMR pathological findings). After 2·2 [0·44] years follow-up, 35·5% of patients suffered the CE: 16% death, 29% cardiovascular event. At multivariate analysis elevated NT-proBNP ≥ 486pg/ml (HR 96·7; 95%-CI 1·135–8243; P = 0·044), reduced native T1 time < 983ms (HR 44·8; 95%-CI 1·235–1623; P = 0·038) and higher left ventricular global longitudinal strain (LV-GLS) (HR 0·4; 95%-CI 0·196–0·973; P = 0·043) showed an independent prognostic value. These variables, together with the myocardial T2* time < 20ms, showed an additive prognostic value (Log Rank: 12·4; P = 0·001). In conclusion, low-risk MDS patients frequently suffer CVD. NT-proBNP value, native T1 relaxation time and longitudinal strain by FT are independent predictors of poor cardiovascular prognosis, thus, their determination would identify high-risk patients who could benefit from a cardiac treatment and follow-up., This work was funded by aGerencia Regional de Salud de Castilla y León grant(GRS1203/A/15) and a Rıo Hortega contract (CM19/00055),supported by the Instituto de Salud Carlos III in Spain (co-funded by the European Social Fund ‘Investing in yourfuture’).

Published
2021
Full Text
View/download PDF

29. Scalable Production of Ambient Stable Hybrid Bismuth‐Based Materials: AACVD of Phenethylammonium Bismuth Iodide Films**

Author

Clara Sanchez-Perez, M. Wang, M. O. Blunt, Claire J. Carmalt, and F. Habib

Subjects
Full Paper, 010405 organic chemistry, Band gap, Chemistry, Organic Chemistry, chemistry.chemical_element, Heterojunction, General Chemistry, Chemical vapor deposition, Full Papers, light-harvesting, 010402 general chemistry, 01 natural sciences, Catalysis, 0104 chemical sciences, Bismuth, Characterization (materials science), lead-free hybrid material, Band bending, Chemical engineering, phenethylammonium bismuth iodide, Hybrid material, scalability, Deposition (law)
Abstract

Large homogeneous and adherent coatings of phenethylammonium bismuth iodide were produced using the cost‐effective and scalable aerosol‐assisted chemical vapor deposition (AACVD) methodology. The film morphology was found to depend on the deposition conditions and substrates, resulting in different optical properties to those reported from their spin‐coated counterparts. Optoelectronic characterization revealed band bending effects occurring between the hybrid material and semiconducting substrates (TiO2 and FTO) due to heterojunction formation, and the optical bandgap of the hybrid material was calculated from UV‐visible and PL spectrometry to be 2.05 eV. Maximum values for hydrophobicity and crystallographic preferential orientation were observed for films deposited on FTO/glass substrates, closely followed by values from films deposited on TiO2/glass substrates., Compact and stable bismuth halide‐based hybrid material thin films were grown using aerosol‐assisted chemical vapor deposition. The dependence of structural and optoelectronic properties of phenethylammonium bismuth iodide films were evaluated on varying substrates, deposition temperatures and curing conditions. The morphology and optical band‐bending effects were related to synthetic parameters, and crack‐free films were obtained with high moisture resistance.

Published
2021
Full Text
View/download PDF

30. Early High-Titer Plasma Therapy to Prevent Severe Covid-19 in Older Adults

Author

Eduardo Bergel, Alejandra Debonis, Ignacio Esteban, Sofia Jares Baglivo, Sofia Sol Aranda, Ramiro Manuel Larrea, Jorge Lantos, Nicolás Kreplak, Candela Etchegaray, Yvonne Ritou, Mabel Berrueta, Ricardo Valentini, Fernando Althabe, Pablo Cruz, Gabriel Leberzstein, Alvaro Ciganda, Sofia Lujan Laudanno, Damian Alvarez Paggi, Fernando Alvez, Susana Pastor Arguello, Luz Gibbons, Alejandra Hintze, M Laura Oyarvide, Clara Sanchez Yanotti, Miguel Santiago Gonzalez, Romina Libster, Gaston Ofman, Federico Cesar Etchenique, Rocio Lopez Castelo, Cristian Wood, Fernando P. Polack, Maria Dolores Silveyra, Alejandra Neira, Ivonne Name, Sebastian Esperante, Gisela Caruso, Nicolas Itcovici, Julian De Luca, Silvina Coviello, Anibal Rondan, Rocio Rodriguez, Valeria Fernandez Vina, Gabriela Lescano, Gonzalo Pérez Marc, Federico Dimase, Dario Raul Alvarez, Virginia Braem, Alejandra Bianchi, Julieta Alfonso, Sofia Rapelius, Adrián Ferreti, Eduardo Perez, Mauricio T. Caballero, Juan Molinos, Diego Wappner, and Florencia Nowogrodzki

Subjects
Relative risk reduction, medicine.medical_specialty, Respiratory rate, Population, 030204 cardiovascular system & hematology, Placebo, law.invention, Plasma, purl.org/becyt/ford/3.3 [https], 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, law, Internal medicine, Severity of illness, medicine, Humans, 030212 general & internal medicine, education, COVID-19 Serotherapy, education.field_of_study, SARS-CoV-2, Transfusion Medicine, business.industry, Respiratory disease, Immunization, Passive, COVID-19, General Medicine, medicine.disease, Hospitals, Older adults, Relative risk, purl.org/becyt/ford/3 [https], Original Article, business
Abstract

BACKGROUND: Therapies to interrupt the progression of early coronavirus disease 2019 (Covid-19) remain elusive. Among them, convalescent plasma administered to hospitalized patients has been unsuccessful, perhaps because antibodies should be administered earlier in the course of illness. METHODS We conducted a randomized, double-blind, placebo-controlled trial of convalescent plasma with high IgG titers against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in older adult patients within 72 hours after the onset of mild Covid-19 symptoms. The primary end point was severe respiratory disease, defined as a respiratory rate of 30 breaths per minute or more, an oxygen saturation of less than 93% while the patient was breathing ambient air, or both. The trial was stopped early at 76% of its projected sample size because cases of Covid-19 in the trial region decreased considerably and steady enrollment of trial patients became virtually impossible. RESULTS A total of 160 patients underwent randomization. In the intention-to-treat population, severe respiratory disease developed in 13 of 80 patients (16%) who received convalescent plasma and 25 of 80 patients (31%) who received placebo (relative risk, 0.52; 95% confidence interval [CI], 0.29 to 0.94; P = 0.03), with a relative risk reduction of 48%. A modified intention-to-treat analysis that excluded 6 patients who had a primary end-point event before infusion of convalescent plasma or placebo showed a larger effect size (relative risk, 0.40; 95% CI, 0.20 to 0.81). No solicited adverse events were observed. CONCLUSIONS Early administration of high-titer convalescent plasma against SARS-CoV-2 to mildly ill infected older adults reduced the progression of Covid-19. (Funded by the Bill and Melinda Gates Foundation and the Fundación INFANT Pandemic Fund; Dirección de Sangre y Medicina Transfusional del Ministerio de Salud number, PAEPCC19, Plataforma de Registro Informatizado de Investigaciones en Salud number, 1421, and ClinicalTrials.gov number, NCT04479163.). Fil: Libster, Romina Paula. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Pérez Marc, Gonzalo. Hospital Militar Central, Buenos Aires; Argentina Fil: Wappner, Diego. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Coviello, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Bianchi, Alejandra. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Braem, Virginia. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Esteban, Ignacio. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Caballero, Mauricio Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wood, Cristian. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Berrueta, Mabel. Hospital Militar Central; Argentina Fil: Rondan, Aníbal. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Lescano, Gabriela Mariel. Hospital Dr. Carlos Bocalandro; Argentina Fil: Cruz, Pablo. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Ritou, Yvonne. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Fernández Viña, Valeria Silvina. Hospital Simplemente Evita; Argentina Fil: Álvarez Paggi, Damián Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Esperante, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Ferreti, Adrián. Hospital Dr. Carlos Bocalandro; Argentina Fil: Ofman, Gaston. University of Oklahoma; Estados Unidos Fil: Ciganda, Álvaro. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal Especializado de Agudos y Cronicos San Juan de Dios.; Argentina Fil: Rodriguez, Rocío. Hospital Simplemente Evita; Argentina Fil: Lantos, Jorge. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Valentini, Ricardo. No especifíca; Fil: Itcovici, Nicolás. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Hintze, Alejandra. No especifíca; Fil: Oyarvide, M. Laura. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Etchegaray, Candela. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Neira, Alejandra. Instituto de Efectividad Clínica y Sanitaria; Argentina Fil: Name, Ivonne. Instituto de Efectividad Clínica y Sanitaria; Argentina Fil: Alfonso, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Swiss Medical Group; Argentina Fil: López Castelo, Rocío. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina Fil: Caruso, Gisela. Hospital Militar Central; Argentina Fil: Rapelius, Sofía. Hospital Militar Central; Argentina Fil: Alvez, Fernando. Hospital Militar Central; Argentina Fil: Etchenique, Federico. Hospital Militar Central; Argentina Fil: Dimase, Federico. Hospital Militar Central; Argentina Fil: Alvarez, Darío. Hospital Militar Central; Argentina Fil: Aranda, Sofía S.. Hospital Militar Central; Argentina Fil: Sánchez Yanotti, Clara Inés. Hospital Militar Central; Argentina Fil: De Luca, Julián. Hospital Militar Central; Argentina Fil: Jares Baglivo, Sofía. Hospital Militar Central; Argentina Fil: Laudanno, Sofía. Fundación Hematológica Sarmiento; Argentina Fil: Nowogrodzki, Florencia. Swiss Medical Group; Argentina Fil: Larrea, Ramiro. Hospital Municipal San Isidro; Argentina Fil: Silveyra, María. Hospital Militar Central; Argentina Fil: Leberzstein, Gabriel. No especifíca; Fil: Debonis, Alejandra. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Molinos, Juan. Fundación para la Investigación en Infectología Infantil; Argentina Fil: González, Miguel. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Perez, Eduardo. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Kreplak, Nicolás. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Pastor Argüello, Susana. Fundación para la Investigación en Infectología Infantil; Argentina Fil: Gibbons, Luz. Hospital Municipal de San Isidro; Argentina Fil: Althabe, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina Fil: Bergel, Eduardo. Sanatorio Sagrado Corazón; Argentina Fil: Polack, Fernando Pedro. Provincia de Buenos Aires. Ministerio de Salud; Argentina

Published
2021
Full Text
View/download PDF

31. TOP-002 Validation of the gender-equity model for liver allocation (GEMA) in a nationwide cohort of liver transplant candidates in Spain

Author

Rodríguez-Perálvarez, Manuel, de la Rosa, Gloria, Gómez-Orellana, Antonio M., Sancho, Victoria Aguilera, Pascual-Vicente, Teresa, Pereira, Sheila, Ortiz, María Luisa, Pagano, Giulia, Suárez, Francisco, González-Grande, Rocio, Cachero, Alba, Tomé, Santiago, Valbuena, Mónica Barreales, Martin-Mateos, Rosa, Pascual, Sonia, Cristóbal, Mario Romero, Bilbao, Itxarone, Martin, Carmen Alonso, Oton, Elena, Dieguez, Maria Luisa Gonzalez, Aguilar, María Dolores Espinosa, Arias, Ana, Blanco, Gerardo, Perez, Sara Lorente, Cuadrado, Antonio, García, Amaya Redín, Cano, Clara Sánchez, Franco, Carmen Cepeda, Pons, Jose Antonio, Colmenero, Jordi, Ferreiro, Alejandra Otero, Aretxabaleta, Nerea Hernández, Moreno, Sarai Romero, Soler, Maria Rodriguez, Hervás, César, and Gastaca, Mikel

Published
2024
Full Text
View/download PDF

35. DIAGNOSIS OF STATE SPORTS INCENTIVE LAWS IN BRAZIL

Author

Vitório, Sabrina de Lima, Cerbi, Leandro Eduardo, Lindman Bueno, Bruna, Santos, Clara Sanchez da Trindade, and Mazzei, Leandro Carlos

Subjects
Políticas Públicas, Leis Estaduais de Incentivo ao Esporte, Esporte, Política Pública, Public Policy, Deporte, Tax Exemption Laws, Leyes de Exención de Impuestos, Sport
Abstract

With the creation of the Federal Sports Incentive Law in 2006 and the Sporting Mega-Events held in Brazil, many states have created their own initiatives related to tax exemption to favor Sports. However, the bibliography on this phenomenon is scarce. Therefore, this study aimed to present a diagnosis of the Sports Incentive Laws at the State level, indicating the actions carried out by the federative units and their main characteristics. This is a descriptive study, using documental sources for data collection obtained through the official websites of each Secretariat or similar body responsible for the Sports Public Policies in the Brazilian States. Were identified 15 States actions related to the incentive to sports via tax exemption and all of them from the ‘ICMS’ tribute. It was also verified a large variation regarding the percentages of value allocated, as well as a great diversification of the sports manifestations in each State., Con la creación de la Ley Federal de Incentivo al Deporte y con los Mega-Eventos Deportivos en Brasil, muchos Estados Brasileños crearon sus iniciativas para favorecer el deporte a través de exenciones fiscales. Sin embargo, la bibliografía sobre este fenómeno es escasa. Por lo tanto, este estudio tuvo como objetivo presentar un diagnóstico de las Leyes de Incentivo al Deporte a nivel de los Estados Brasileños, indicando sus principales características. Se trata de un estudio descriptivo, que utiliza fuentes documentales obtenidos a través de los sitios web oficiales de cada Secretaría u organismo similar responsable de las Políticas Públicas de Deporte en los Estados. Se identificaron acciones relacionadas con el incentivo desportivo en 15 Estados y todos ellos relacionados com el impuestos ICMS. También se verificó una gran variación en cuanto a los porcentajes de valor destinados, así como una diversificación de las manifestaciones deportivas en cada Estado., Com a criação da Lei Federal de Incentivo ao Esporte em 2006 e na onda dos Megaeventos Esportivos no Brasil, muitos Estados criaram suas próprias iniciativas relacionadas à isenção fiscal para favorecimento do Esporte. No entanto, a bibliografia sobre este fenômeno é escassa. Assim, este estudo teve por objetivo apresentar um diagnóstico das Leis de Incentivo ao Esporte (LIEs) no âmbito Estadual, indicando as características destas ações realizadas pelas unidades federativas. Trata-se de um estudo de caráter descritivo, utilizando de fontes documentais para a coleta de dados obtidas através dos sites oficiais de cada Secretaria ou órgão semelhante responsável pelas Políticas Públicas do Esporte nos Estados. Foram identificadas ações relacionadas ao incentivo do esporte via isenção fiscal em 15 Estados e todas envolvem o tributo ICMS. Verificou-se ainda uma variação grande com relação às porcentagens de valor destinadas, bem como uma diversificação das manifestações esportivas em cada Estado.

Published
2021
Full Text
View/download PDF

39. Aerosol-assisted route to low-E transparent conductive gallium-doped zinc oxide coatings from pre-organized and halogen-free precursor

Author

Jawwad A. Darr, Clara Sanchez-Perez, Claire J. Carmalt, Ivan P. Parkin, and Sebastian C. Dixon

Subjects
Materials science, business.industry, Band gap, Doping, chemistry.chemical_element, General Chemistry, Zinc, Chemical vapor deposition, Epitaxy, Chemistry, Glazing, chemistry, Optoelectronics, Charge carrier, Gallium, business
Abstract

Thermal control in low-emission windows is achieved by the application of glazings, which are simultaneously optically transparent in the visible and reflective in the near-infrared (IR). This phenomenon is characteristic of coatings with wide optical band gaps that have high enough charge carrier concentrations for the material to interact with electromagnetic radiation in the IR region. While conventional low-E coatings are composed of sandwiched structures of oxides and thin Ag films or of fluorinated SnO2 coatings, ZnO-based glazing offers an environmentally stable and economical alternative with competitive optoelectronic properties. In this work, gallium-doped zinc oxide (GZO) coatings with properties for low-E coatings that exceed industrial standards (Tvisible > 82%; R2500 nm > 90%; λ(plasma) = 1290 nm; ρ = 4.7 × 10−4 Ω cm; Rsh = 9.4 Ω·□−1) are deposited through a sustainable and environmentally friendly halogen-free deposition route from [Ga(acac)3] and a pre-organized zinc oxide precursor [EtZnOiPr]4 (1) via single-pot aerosol-assisted chemical vapor deposition. GZO films are highly (002)-textured, smooth and compact without need of epitaxial growth. The method herein describes the synthesis of coatings with opto-electronic properties commonly achievable only through high-vacuum methods, and provides an alternative to the use of pyrophoric ZnEt2 and halogenated SnO2 coatings currently used in low-emission glazing and photovoltaic technology., A pre-organised and halogen-free ZnO precursor is used to deposit GZO coatings via aerosol-assisted CVD with properties that exceed industrial standards for low-E coatings and photovoltaics.

Published
2020
Full Text
View/download PDF

40. Dual knockdown of Galectin-8 and its glycosylated ligand, the activated leukocyte cell adhesion molecule (ALCAM/CD166), synergistically delays in vivo breast cancer growth

Author

Karina Mariño, Fátima Ferragut, María T. Elola, Silvia Vanzulli, Lucas L. Colombo, Clara Sanchez Terrero, Gabriel A. Rabinovich, María F. Troncoso, Carlota Wolfenstein-Todel, and Alejandro J. Cagnoni

Subjects
Fetal Proteins, 0301 basic medicine, CIENCIAS MÉDICAS Y DE LA SALUD, Cell Adhesion Molecules, Neuronal, Galectins, Triple Negative Breast Neoplasms, Mice, 03 medical and health sciences, 0302 clinical medicine, Antigens, CD, Cell Line, Tumor, Cell Adhesion, TRIPLE NEGATIVE BREAST CANCER, Animals, Humans, Cell adhesion, Receptor, Molecular Biology, ALCAM, Galectin, ALCAM (CD166), Chemistry, GALECTIN-8, SIALYLATION, Otras Medicina Básica, Activated-Leukocyte Cell Adhesion Molecule, CELL ADHESION AND MIGRATION, purl.org/becyt/ford/3.1 [https], Cell Biology, Adhesion, N-GLYCOSYLATION, Neoplasm Proteins, Cell biology, Medicina Básica, Galectin-8, 030104 developmental biology, Gene Knockdown Techniques, 030220 oncology & carcinogenesis, Immunoglobulin superfamily, purl.org/becyt/ford/3 [https], Female, TUMOR GROWTH
Abstract

Galectin-8 (Gal-8), a ‘tandem-repeat’-type galectin, has been described as a modulator of cellular functions including adhesion, spreading, growth arrest, apoptosis, pathogen recognition, autophagy, and immunomodulation. We have previously shown that activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, serves as a receptor for endogenous Gal-8. ALCAM is a member of the immunoglobulin superfamily involved in cell-cell adhesion through homophilic (ALCAM-ALCAM) and heterophilic (i.e. ALCAM-CD6) interactions in different tissues. Here we investigated the physiologic relevance of ALCAM-Gal-8 association and glycosylation-dependent mechanisms governing these interactions. We found that silencing of ALCAM in MDA-MB-231 triple negative breast cancer cells decreases cell adhesion and migration onto Gal-8-coated surfaces in a glycan-dependent fashion. Remarkably, either Gal-8 or ALCAM silencing also disrupted cell-cell adhesion, and led to reduced tumor growth in a murine model of triple negative breast cancer. Moreover, structural characterization of endogenous ALCAM N-glycosylation showed abundant permissive structures for Gal-8 binding. Importantly, we also found that cell sialylation controls Gal-8-mediated cell adhesion. Altogether, these findings demonstrate a central role of either ALCAM or Gal-8 (or both) in controlling triple negative breast cancer. Fil: Ferragut, Fatima Eneida del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Cagnoni, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Colombo, Lucas Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina Fil: Sánchez Terrero, Clara. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina Fil: Wolfenstein Todel, Carlota. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Troncoso, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Mariño, Karina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Elola, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina

Published
2019
Full Text
View/download PDF

41. The Neuropeptide Cortistatin Alleviates Neuropathic Pain in Experimental Models of Peripheral Nerve Injury

Author

Raquel Benitez, Pedro Hernández-Cortés, Mario Delgado, Marta Caro, Elena Gonzalez-Rey, CP Falo, Francisco O'Valle, Clara Sanchez-Gonzalez, Maria Morell, and Irene Forte-Lago

Subjects
Pharmaceutical Science, Pharmacology, Article, neuroinflammation, 03 medical and health sciences, 0302 clinical medicine, Pharmacy and materia medica, medicine, peripheral nerve injury, neuropeptide, neuroregeneration, 030304 developmental biology, neuropathic pain, 0303 health sciences, business.industry, Chronic pain, Nerve injury, medicine.disease, Cortistatin, RS1-441, Allodynia, Nociception, Peripheral nerve injury, Hyperalgesia, Neuropathic pain, neuroprotection, medicine.symptom, business, 030217 neurology & neurosurgery
Abstract

Neuropathic pain is one of the most severe forms of chronic pain caused by the direct injury of the somatosensory system. The current drugs for treating neuropathies have limited efficacies or show important side effects, and the development of analgesics with novel modes of action is critical. The identification of endogenous anti-nociceptive factors has emerged as an attractive strategy for designing new pharmacological approaches to treat neuropathic pain. Cortistatin is a neuropeptide with potent anti-inflammatory activity, recently identified as a natural analgesic peptide in several models of pain evoked by inflammatory conditions. Here, we investigated the potential analgesic effect of cortistatin in neuropathic pain using a variety of experimental models of peripheral nerve injury caused by chronic constriction or partial transection of the sciatic nerve or by diabetic neuropathy. We found that the peripheral and central injection of cortistatin ameliorated hyperalgesia and allodynia, two of the dominant clinical manifestations of chronic neuropathic pain. Cortistatin-induced analgesia was multitargeted, as it regulated the nerve damage-induced hypersensitization of primary nociceptors, inhibited neuroinflammatory responses, and enhanced the production of neurotrophic factors both at the peripheral and central levels. We also demonstrated the neuroregenerative/protective capacity of cortistatin in a model of severe peripheral nerve transection. Interestingly, the nociceptive system responded to nerve injury by secreting cortistatin, and a deficiency in cortistatin exacerbated the neuropathic pain responses and peripheral nerve dysfunction. Therefore, cortistatin-based therapies emerge as attractive alternatives for treating chronic neuropathic pain of different etiologies.

Published
2021

42. Increasing detections of the margay: occupancy, density, and activity patterns in Madre de Dios, Peru

Author

Samantha Zwicker, Clara Sánchez-Latorre, and Clemens Lukasser

Subjects
behavioral ecology, home ranges, Leopardus wiedii, occupancy modeling, semiarboreal camera, spatial capture-recapture, Evolution, QH359-425, Ecology, QH540-549.5
Abstract

This study provides novel insights into the ecology of the margay (Leopardus wiedii), focusing on its occupancy, density, and activity patterns in the Madre de Dios region, Peru, by utilizing both arboreal and terrestrial camera traps. Conducted across 10 km2, the research achieved 47 detections, utilizing semi-arboreal, lower canopy cameras for the first time to capture margay activities. Occupancy models revealed a mean occupancy probability of 53.82% and a detection probability of 6.57%. Among the environmental covariates, diameter at breast height (DBH) was identified as a significant predictor, negatively impacting occupancy, suggesting margays favor areas with smaller tree diameters. Contrary to expectations, the Normalized Difference Vegetation Index (NDVI) did not significantly influence occupancy. Tree density exhibited a positive, though non-significant, association with margay presence. Spatial capture-recapture (SECR) models estimated a margay density of 71.46 individuals per 100 km², with significant sex-based differences in spatial behavior. Males demonstrated larger home ranges (approximately 13.50 km²) compared to females (approximately 3.79 km²). Activity pattern analyses indicated primary nocturnal behavior with peaks at midnight to 3 am, 5 am, and 6 pm. Temporal overlap analysis revealed a low overlap coefficient with jaguarundis (Dhat1 = 0.21) and a higher overlap with ocelots (Dhat4 = 0.79), reflecting intricate interspecies dynamics. Our findings highlight important ecological aspects of margay behavior, including habitat preferences, nocturnal activity patterns, and interspecies interactions, which were effectively captured through the combined use of terrestrial and arboreal camera traps. The study emphasizes the importance of habitat preservation and the development of conservation strategies tailored to the ecological needs of margays, potentially influencing global practices for the management and protection of lesser-studied semi-arboreal wild cats.

Published
2024
Full Text
View/download PDF

43. Sustained antiviral response against in vitro HIV-1 infection in peripheral blood mononuclear cells from people with chronic myeloid leukemia treated with ponatinib

Author

Mario Manzanares, Fernando Ramos-Martín, Sara Rodríguez-Mora, Guiomar Casado-Fernández, Clara Sánchez-Menéndez, Alicia Simón-Rueda, Elena Mateos, Miguel Cervero, Adam M. Spivak, Vicente Planelles, Montserrat Torres, Valentín García-Gutiérrez, and Mayte Coiras

Subjects
HIV-1, ponatinib, cytotoxic immunity, antiviral response, chronic myeloid leukemia, Therapeutics. Pharmacology, RM1-950
Abstract

HIV-1 infection cannot be cured due to long-lived viral reservoirs formed by latently infected CD4+ T cells. “Shock and Kill” strategy has been considered to eliminate the viral reservoir and achieve a functional cure but the stimulation of cytotoxic immunity is necessary. Ponatinib is a tyrosine kinase inhibitor (TKI) clinically used against chronic myeloid leukemia (CML) that has demonstrated to be effective against HIV-1 infection in vitro. Several TKIs may induce a potent cytotoxic response against cancer cells that makes possible to discontinue treatment in people with CML who present long-term deep molecular response. In this longitudinal study, we analyzed the capacity of ponatinib to induce an antiviral response against HIV-1 infection in peripheral blood mononuclear cells (PBMCs) obtained from people with CML previously treated with imatinib for a median of 10 years who changed to ponatinib for 12 months to boost the anticancer response before discontinuing any TKI as part of the clinical trial NCT04043676. Participants were followed-up for an additional 12 months in the absence of treatment. PBMCs were obtained at different time points and then infected in vitro with HIV-1. The rate of infection was determined by quantifying the intracellular levels of p24-gag in CD4+ T cells. The levels of p24-gag+ CD4+ T−cells were lower when these cells were obtained during and after treatment with ponatinib in comparison with those obtained during treatment with imatinib. Cytotoxicity of PBMCs against HIV-infected target cells was significantly higher during treatment with ponatinib than during treatment with imatinib, and it was maintained at least 12 months after discontinuation. There was a significant negative correlation between the lower levels of p24-gag+ CD4+ T−cells and the higher cytotoxicity induced by PBMCs when cells were obtained during and after treatment with ponatinib. This cytotoxic immunity was mostly based on higher levels of Natural Killer and Tγδ cells seemingly boosted by ponatinib. In conclusion, transient treatment with immunomodulators like ponatinib along with ART could be explored to boost the antiviral activity of cytotoxic cells and contribute to the elimination of HIV-1 reservoir.

Published
2024
Full Text
View/download PDF

44. Advances in the development of high efficiency III-V multijunction solar cells on Ge|Si virtual substrates

Author

Pere Roca i Cabarrocas, Victor Orejuela, Manuel Hinojosa, Monalisa Ghosh, Iván García, Clara Sanchez, Shabnam Dadgostar, and Ignacio Rey-Stolle

Subjects
Silicon, Materials science, business.industry, Germanium, Triple junction, Germanio, chemistry.chemical_element, Multijunction photovoltaic cell, Silicio, Células fotovoltaicas multiunión, chemistry, Optoelectronics, Multijunction solar cells, business, Absorption (electromagnetic radiation), Porosity, Layer (electronics), Deposition (law)
Abstract

Producción Científica, Virtual Ge substrates fabricated by direct deposition of Ge on Si have become a pathway with high potential to attain high-efficiency III-V multijunction solar cells on Si. We study the development of III-V triple junction solar cells using two types of Ge|Si virtual substrates. The first uses a thick (2-5 μm) Ge layer grown by CVD, which acts as the bottom Ge subcell. The second, grown by low-temperature RT-PECVD, has a thickness of a few tens of nanometres, with the Si substrate acting as Si bottom cell. We discuss the challenges related to each design (formation of cracks, parasitic absorption in the Ge layer, dislocations, ...), present the theoretical design and show the experimental results obtained. Finally, an advanced approach using embedded porous Si layers as buffer layers for crack mitigation is also presented., Ministerio de Ciencia, Innovación y Universidades (project RTI2018-094291-B-I00), Comunidad de Madrid (project S2018/EMT-4308), Ministerio de Ciencia e Innovación (grant PRE2019-088437), Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (project VA283P18)

Published
2021
Full Text
View/download PDF

45. The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development

Author

Mathie Tenenbaum, Amar Abderrahmani, Amélie Bonnefond, Stéphane Dalle, Julien Bourry, Valery Gmyr, Philippe Froguel, Valérie Pawlowski, Nicole Beeler, Valérie Plaisance, Raphael Boutry, Hélène Ezanno, Clara Sanchez-Parra, Cécile Jacovetti, Romano Regazzi, Gianni Pasquetti, François Pattou, Syu-Ichi Hirai, Julie Kerr-Conte, Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (EGENODIA (GI3M)), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université de Lausanne = University of Lausanne (UNIL), Recherche translationnelle sur le diabète - U 1190 (RTD), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Wakayama University, Imperial College London, This work was supported by grants from 'Société Francophone du Diabète (SFD)', 'European Genomic Institute for Diabetes' (E.G.I.D., ANR-10-LABEX-46) and European Commission, European Research Council (GEPIDIAB 294785 to P.F.), and by a grant from the Swiss National Science Foundation (310030-169480 to RR). Human islets were provided by the European Consortium for Islet Transplantation, funded by the Juvenile Diabetes Research Foundation International., ANR-10-LABX-0046,EGID,EGID Diabetes Pole(2010), European Project: 294785,EC:FP7:ERC,ERC-2011-ADG_20110310,GEPIDIAB(2012), Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (GI3M), Université de Lausanne (UNIL), and Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)

Subjects
MAPK/ERK pathway, endocrine system, Biology, Mapk, Beta-cell mass, Transcriptome, Rats, Sprague-Dawley, 03 medical and health sciences, Cellular and Molecular Neuroscience, Mice, [SPI]Engineering Sciences [physics], Mitogen-Activated Protein Kinase 10, Pregnancy, Insulin-Secreting Cells, Animals, Cell Proliferation/drug effects, Cyclin D1/genetics, Cyclin D1/metabolism, Cyclin D2/genetics, Cyclin D2/metabolism, Female, Glucose/pharmacology, Humans, Insulin/metabolism, Insulin-Secreting Cells/cytology, Insulin-Secreting Cells/metabolism, MAP Kinase Kinase Kinases/antagonists & inhibitors, MAP Kinase Kinase Kinases/genetics, MAP Kinase Kinase Kinases/metabolism, Mice, Inbred C57BL, Mitogen-Activated Protein Kinase 10/antagonists & inhibitors, Mitogen-Activated Protein Kinase 10/genetics, Mitogen-Activated Protein Kinase 10/metabolism, Obesity/metabolism, Obesity/pathology, Pancreas/growth & development, Pancreas/metabolism, RNA Interference, RNA, Small Interfering/metabolism, Rats, Signal Transduction/drug effects, Obesity, Postnatal development, Gene silencing, Cyclin D2, Insulin, Cyclin D1, RNA, Small Interfering, Molecular Biology, Pancreas, Cyclin, Cell Proliferation, Pharmacology, Serine/threonine-specific protein kinase, 0303 health sciences, geography, geography.geographical_feature_category, Kinase, 030302 biochemistry & molecular biology, Cell Biology, Islet, MAP Kinase Kinase Kinases, Cell biology, Glucose, Molecular Medicine, Signal transduction, Signal Transduction
Abstract

International audience; Unveiling the key pathways underlying postnatal beta-cell proliferation can be instrumental to decipher the mechanisms of beta-cell mass plasticity to increased physiological demand of insulin during weight gain and pregnancy. Using transcriptome and global Serine Threonine Kinase activity (STK) analyses of islets from newborn (10 days old) and adult rats, we found that highly proliferative neonatal rat islet cells display a substantially elevated activity of the mitogen activated protein 3 kinase 12, also called dual leucine zipper-bearing kinase (Dlk). As a key upstream component of the c-Jun amino terminal kinase (Jnk) pathway, Dlk overexpression was associated with increased Jnk3 activity and was mainly localized in the beta-cell cytoplasm. We provide the evidence that Dlk associates with and activates Jnk3, and that this cascade stimu- lates the expression of Ccnd1 and Ccnd2, two essential cyclins controlling postnatal beta-cell replication. Silencing of Dlk or of Jnk3 in neonatal islet cells dramatically hampered primary beta-cell replication and the expression of the two cyclins. Moreover, the expression of Dlk, Jnk3, Ccnd1 and Ccnd2 was induced in high replicative islet beta cells from ob/ob mice during weight gain, and from pregnant female rats. In human islets from non-diabetic obese individuals, DLK expression was also cytoplasmic and the rise of the mRNA level was associated with an increase of JNK3, CCND1 and CCND2 mRNA levels, when compared to islets from lean and obese patients with diabetes. In conclusion, we fjnd that activation of Jnk3 signalling by Dlk could be a key mechanism for adapting islet beta-cell mass during postnatal development and weight gain.

Published
2021
Full Text
View/download PDF

46. Prevention of severe COVID-19 in the elderly by early high-titer plasma

Author

Rocio Rodriguez, Mariano Girasolli, Viviana Dominguez, Valeria Fernandez Vina, Jorge Lantos, Florencia Nowogrodzki, Julian DeLuca, Sofia Lujan Laudanno, Clarisa Taffarel, Florencia Izetta, Maria del Carmen Nigro, Nicolás Kreplak, Fernando Alvez, Laura Oyarvide, Ivonne Ritou, Emmanuel Ezequiel Valls, Dario Raul Alvarez, Patricia Lesch, Indira Pichetto Olanda, Florencia De la Fuente, Camila Witteveen, Sebastian Esperante, Juan Rusconi, Sofia Sol Aranda, Juan Canela, Ricardo Valentini, Mauricio T. Caballero, Clara Sanchez Yanotti, Paula Fernandez Estrella, Gisela Caruso, Patricia Rearte Carvalho, Ramiro Manuel Larrea, Candela Etchegaray, Romina Libster, Cecilia Pampuro, Fernando P. Polack, Jose L Scapellato, Micaela Piani, Julieta Alfonso, Daniela Carolina Galnarez, Maria Eugenia Macaneo, Federico Dimase, Agustina Venditti, Juan Molinos, Cristina Soler Riera, Jimena Ochoa, Luz Gibbons, Eduardo Perez, Rocio Zarlenga, Alejandra Hintze, Guido Ochoa, Damian Alvarez Paggi, Silvina Kuperman, Virginia Braem, Mariano Aizpurua, Alfonso Raggio, Miguel Santiago Gonzalez, Maria Teresa Paniguetti, Maria Fernanda Caracciolo, Gabriela Lescano, Juan Mauricio Vargas, Silvina Coviello, Fernando Althabe, Sofia Jarez Baglivo, Juan Sebastian Riera, Anibal Rondan, Mario Rovere, Gaston Pellegrino, Nicolas Itcovici, Yanina Miragaya, Alejandra Neira, Carla Marchionatti, Silvana Marquez, Maria Emilia Gutierrez Meyer, Gonzalo Pérez Marc, Marcela Balduzzi, Lia Pocket, Mabel Berrueta, Romina Zadoff, Noelia Iraizos, Susana Villaroel, Eduardo Bergel, Rocio Lopez Castelo, Maria Veronica Paz, Maria Dolores Silveyra, Maximiliano de Zan, Agostina Pagella, Lucila DiNunzio, Jimena Franco, Romina Militerno, Natalia Garcia Escude, Gabriela Leberzstein, Diego Wappner, Pablo Cruz, Romina Romero, Jorge Geffner, Susana Pastor Arguello, Leonel Langellotti, Andrea V. Gamarnik, Alejandra Debonis, Ignacio Esteban, Gaston Ofman, Federico Cesar Etchenique, Cristian Wood, Ivonne Name, Natali Estrada, Sebastian Perez Marc, Enio Garcia, Sofia Rapelius, Alvaro Ciganda, Alejandra Bianchi, and Adrian Ferretti

Subjects
medicine.medical_specialty, Randomization, Respiratory rate, business.industry, Respiratory disease, Placebo, medicine.disease, Titer, Internal medicine, Number needed to treat, Clinical endpoint, Medicine, business, Adverse effect
Abstract

BackgroundTherapies to interrupt progression of early COVID-19 remain elusive. Among them, convalescent plasma in hospitalized patients was unsuccessful, perhaps because antibody should be administered earlier. We advanced plasma infusions to the first 72 hours of symptoms to arrest COVID-19 progression.MethodsA randomized, double-blind, placebo-controlled trial of convalescent plasma with high IgG titers against SARS-CoV2 in elderly subjects within 72 hours of mild COVID-19 symptoms. The primary endpoint was severe respiratory disease defined as a respiratory rate ≥30 and/or an O2satResults160 patients underwent randomization. In the intention-to-treat analysis (ITT), 13/80(16.2%) patients receiving plasma vs. 25/80(31.2%) receiving placebo experienced severe respiratory disease [RR(95%CI)= 0.52(0.29,0.94); p=0.026)] with an RRR=48%.A modified ITT analysis, excluding six subjects who experienced the primary endpoint before infusion, showed a larger effect size [RR(95%CI) = 0.40(0.20, 0.81), p=0.007]. High- and low-titer donor analyses, based on a median IgG titer=1:3,200, evidenced a dose-dependent response with an RRR=73.3% for recipients of high-titer plasma (p=0.016) and a number needed to treat (NNT)=4.4. All secondary endpoints exhibited trends towards protection. No solicited adverse events were observed.ConclusionsEarly administration of high-titer convalescent plasma against SARS-CoV2 to mildly ill infected seniors reduced COVID-19 progression. This safe, inexpensive, outpatient intervention facilitates access to treatment from industrialized to LMIC, can decompress demands on hospitals, and may contribute to save lives.Funded by The Bill & Melinda Gates Foundation and The Fundación INFANT Pandemic Fund. Registered in the Dirección de Sangre y Medicina Transfusional del Ministerio de Salud (PAEPCC19), Plataforma PRIISA (1421), and clinicaltrials.gov (NCT04479163).All authors have completed the ICMJE uniform disclosure form atwww.icmje.org/coi_disclosure.pdfand declare: no support from any organization for the submitted work; RL, GPM, DW and FPP are investigators in a phase 3 SARS CoV2 trial from Pfizer; no other relationships or activities that could appear to have influenced the submitted work.

Published
2020
Full Text
View/download PDF

48. Stability and peri-implant bone level of post-extractive implants in elderly patients

Author

Orlando Guerra Cobian, Clara Sanchez Silot, and Jorge Luis García Romero

Subjects
lcsh:R5-920, lcsh:Public aspects of medicine, implantes postextractivos, estabilidad, pérdida ósea periimplantaria, adulto mayor, lcsh:RA1-1270, lcsh:Medicine (General)
Abstract

Introduction: Post-extractive implants shorten the time in achieving the rehabilitation of the patient, being this condition an essential factor to restore the quality of life to elderly patients at short term.Objective: To determine the stability values and peri-implant bone loss in post-extractive implants in elderly patients.Material and Method: A cohort study was carried out in 99 elderly patients at Raúl González Sánchez Dental School of Havana from 2017 to 2019. Under the consent of the patients, 173 post-extractive implants were placed. Bone support type, and primary and secondary stability were determined on the basis of a resonance frequency analysis with Ostell Mentor®. The peri-implant bone level and peri-implant bone loss were measured until 12 months after rehabilitation.Results: Implants were mainly positioned in the maxillary incisive site in 43,3 % of the cases. The average values of primary and secondary stability were 48 ISQ and 68 ISQ, respectively. The average bone loss after 12 months of rehabilitation was 1,04 ± 0,22 mm.Conclusions: Post-extractive dental implants were inserted preferably in the maxillary incisive site and in D2 bone type, registering moderated average values of primary stability and substantial average values of secondary stability. The peri-implant vertical bone loss exhibited implants with similar values than those in the healed areas and within the standardized value for peri-implant bone loss within the first year after implant placement.

Published
2020

50. Precursors for Atmospheric Plasma-Enhanced Sintering: Low-Temperature Inkjet Printing of Conductive Copper

Author

Shreya Mrig, Caroline E. Knapp, Samuel P. Douglas, Patrick Choquet, Clara Sanchez-Perez, Elizabeth A. Metcalf, and Nicolas D. Boscher

Subjects
Thermogravimetric analysis, Denticity, Materials science, plasma-assisted inkjet printing, chemistry.chemical_element, Sintering, Infrared spectroscopy, 02 engineering and technology, 01 natural sciences, chemistry.chemical_compound, Diamine, 0103 physical sciences, 010302 applied physics, inkjet printing, Full Paper, Thermal decomposition, General Chemistry, Full Papers, 021001 nanoscience & nanotechnology, Copper, chemistry, conductive copper, Elemental analysis, Physical chemistry, atmospheric plasma, 0210 nano-technology, copper precursors
Abstract

Bidentate diamine and amino‐alcohol ligands have been used to form solid, water‐soluble, and air‐stable monomeric copper complexes of the type [Cu(NH2CH2CH(R)Y)2(NO3)2] (1, R=H, Y=NH2; 2, R=H, Y=OH; 3, R=Me, Y=OH). The complexes were characterized by elemental analysis, mass spectrometry, infrared spectroscopy, thermal gravimetric analysis, and single‐crystal X‐ray diffraction. Irrespective of their decomposition temperature, precursors 1–3 yield highly conductive copper features [1.5×10−6 Ω m (±5×10−7 Ω m)] upon atmospheric‐pressure plasma‐enhanced sintering.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results