54 results on '"Castrop, Hayo"'
Search Results
2. Renal medullary blood flow and essential hypertension.
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Castrop, Hayo
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ESSENTIAL hypertension , *BLOOD flow , *BLOOD pressure , *VASCULAR resistance , *DISEASE risk factors , *BLOOD volume - Abstract
The article offers information on the renal medullary blood flow as a potential determinant of essential hypertension. It discusses the Hypertension as one of the major risk factors for cardiovascular disease and one cause of mortality. It mentions the limited therapeutic options for treatment of essential hypertension and interventions aim at lowering blood pressure rather than addressing the cause of hypertension.
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- 2019
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3. Deep insights: intravital imaging with two-photon microscopy.
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Schießl, Ina and Castrop, Hayo
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MULTIPHOTON excitation microscopy , *SIGNAL processing , *WILD animal collecting , *IMAGE analysis , *COMPUTER software - Abstract
Intravital multiphoton microscopy is widely used to assess the structure and function of organs in live animals. Although different tissues vary in their accessibility for intravital multiphoton imaging, considerable progress has been made in the imaging quality of all tissues due to substantial technical improvements in the relevant imaging components, such as optics, excitation laser, detectors, and signal analysis software. In this review, we provide an overview of the technical background of intravital multiphoton microscopy. Then, we note a few seminal findings that were made through the use of multiphoton microscopy. Finally, we address the technical limitations of the method and provide an outlook for how these limitations may be overcome through future technical developments. [ABSTRACT FROM AUTHOR]
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- 2016
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4. A role for AT1 receptor-associated proteins in blood pressure regulation.
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Castrop, Hayo
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BIOMOLECULES , *REGULATION of blood pressure , *ANGIOTENSIN converting enzyme , *HOMEOSTASIS , *HUMAN anatomy - Abstract
The renin angiotensin-system is one of the most important humoral regulators of blood pressure. The recently discovered angiotensin receptor-associated proteins serve as local modulators of the renin angiotensin-system. These proteins interact with the AT1 receptor in a tissue-specific manner and regulate the sensitivity of the target cell for angiotensin II. The predominant effect of the AT1 receptor-associated proteins on angiotensin II-induced signaling is the modulation of the surface expression of the AT1 receptor. This review provides an overview of our current knowledge with respect to the relevance of AT1 receptor-associated proteins for blood pressure regulation. Two aspects of blood pressure regulation will be discussed in detail: angiotensin II-dependent volume homoeostasis and vascular resistance. [ABSTRACT FROM AUTHOR]
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- 2015
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5. Physiology and pathophysiology of the renal Na-K-2Cl cotransporter (NKCC2).
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Castrop, Hayo and Schießl, Ina Maria
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CARRIER proteins , *PROTEIN binding , *PARASITE antigens , *EPITHELIAL cells , *PATHOLOGICAL physiology , *PHYSIOLOGICAL research , *CELL physiology - Abstract
The Na-K-2Cl cotransporter (NKCC2; BSC1) is located in the apical membrane of the epithelial cells of the thick ascending limb of the loop of Henle (TAL). NKCC2 facilitates ∼20-25% of the reuptake of the total filtered NaCl load. NKCC2 is therefore one of the transport proteins with the highest overall reabsorptive capacity in the kidney. Consequently, even subtle changes in NKCC2 transport activity considerably alter the renal reabsorptive capacity for NaCl and eventually lead to perturbations of the salt and water homoeostasis. In addition to facilitating the bulk reabsorption of NaCl in the TAL, NKCC2 transport activity in the macula densa cells of the TAL constitutes the initial step of the tubular-vascular communication within the juxtaglomerular apparatus (JGA); this communications allows the TAL to modulate the preglomerular resistance of the afferent arteriole and the renin secretion from the granular cells of the JGA. This review provides an overview of our current knowledge with respect to the general functions of NKCC2, the modulation of its transport activity by different regulatory mechanisms, and new developments in the pathophysiology of NKCC2-dependent renal NaCl transport. [ABSTRACT FROM AUTHOR]
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- 2014
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6. Effects of NKCC2 isoform regulation on NaCl transport in thick ascending limb and macula densa: a modeling study.
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Edwards, Aurélie, Castrop, Hayo, Laghmani, Kamel, Vallon, Volker, and Layton, Anita T.
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SODIUM-potassium-chloride cotransporters , *KIDNEY physiology , *REGULATION of blood pressure , *RNA splicing , *HOMEOSTASIS , *MATHEMATICAL models - Abstract
This study aims to understand the extent to which modulation of the Na+-K+-2Cl- cotransporter NKCC2 differential splicing affects NaCl delivery to the macula densa. NaCl absorption by the thick ascending limb and macula densa cells is mediated by apical NKCC2. A recent study has indicated that differential splicing of NKCC2 is modulated by dietary salt (Schiel IM, Rosenauer A, Kattler V, Minuth WW, Oppermann M, Castrop H. Am J Physiol Renal Physiol 305: F1139-F1148, 2013). Given the markedly different ion affinities of its splice variants, modulation of NKCC2 differential splicing is believed to impact NaCl reabsorption. To assess the validity of that hypothesis, we have developed a mathematical model of macula densa cell transport and incorporated that cell model into a previously applied model of the thick ascending limb (Weinstein AM, Krahn TA. Am J Physiol Renal Physiol 298: F525-F542, 2010). The macula densa model predicts a 27.4- and 13.1-mV depolarization of the basolateral membrane [as a surrogate for activation of tubuloglomerular feedback (TGF)] when luminal NaCl concentration is increased from 25 to 145 mM or luminal K concentration is increased from 1.5 to 3.5 mM, respectively, consistent with experimental measurements. Simulations indicate that with luminal solute concentrations consistent with in vivo conditions near the macula densa, NKCC2 operates near its equilibrium state. Results also suggest that modulation of NKCC2 differential splicing by low salt, which induces a shift from NKCC2-A to NKCC2-B primarily in the cortical thick ascending limb and macula densa cells, significantly enhances salt reabsorption in the thick limb and reduces Na+ and Cl- delivery to the macula densa by 3.7 and 12.5%, respectively. Simulation results also predict that the NKCC2 isoform shift hyperpolarizes the macula densa basolateral cell membrane, which, taken in isolation, may inhibit the release of the TGF signal. However, excessive early distal salt delivery and renal salt loss during a low-salt diet may be prevented by an asymmetric TGF response, which may be more sensitive to flow increases. [ABSTRACT FROM AUTHOR]
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- 2014
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7. Angiotensin II AT2 receptor activation attenuates AT1 receptor-induced increases in the glomerular filtration of albumin: a multiphoton microscopy study.
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Maria Schießl, Ina and Castrop, Hayo
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ANGIOTENSIN II , *ANGIOTENSIN receptors , *TRANCE protein , *GLOMERULAR filtration rate , *ALBUMINS , *MULTIPHOTON excitation microscopy , *LABORATORY rats - Abstract
Schießl IM, Castrop H. Angiotensin II AT2 receptor activation attenuates AT1 receptor-induced increases in the glomerular filtration of albumin: a multiphoton microscopy study. Am J Physiol Renal Physiol 305: F1189-F1200, 2013. First published August 14, 2013; doi:10.1152/ajprenal.00377.2013.--In this study, we assessed the acute effects of angiotensin II on the albumin glomerular sieving coefficient (GSC) using intravital microscopy. The experiments were performed on Munich Wistar Froemter (MWF) rats. Alexa-Fluor-594 albumin was injected intravenously, and the fluorescence intensity in the glomerular capillaries and Bowman's space was determined to calculate the albumin GSC. The GSC was measured before and during the constant infusion of angiotensin II (10 ng·min-1·kg-1 body wt). Baseline mean arterial pressure (MAP) was 99 ± 5 mmHg and stabilized at 137 ± 5 mmHg during angiotensin II infusion. The baseline GSC averaged 0.00044 ± 4.8 * 10-5 and increased by 286 ± 44% after angiotensin II infusion (P < 0.0001). The proximal tubular Alexa-Fluor-594 albumin uptake was enhanced during angiotensin II infusion (518% of the baseline value during angiotensin II vs. 218% in controls; P < 0.0001). No change in GSC was observed when the AT1 antagonist losartan was injected before the start of angiotensin II infusion. The AT2 antagonist PD123319 increased the baseline GSC from 0.00052 ± 3.6 * 10-5 to 0.00074 ± 8.2 * 10-5 (P < 0.02) without altering the MAP. During angiotensin II infusion with losartan, PD123319 increased the albumin GSC from 0.00037 ± 5.8 * 10-5 to 0.00115 ± 0.00015 (P < 0.001). When the renal perfusion pressure was mechanically controlled, the GSC increased from 0.0007 ± 0.00019 to 0.0025 ± 0.00063 during angiotensin II infusion (P < 0.047), similar to what was observed when the renal perfusion pressure was allowed to increase. In summary, AT1 activation acutely increases the albumin GSC. This effect appears to be largely independent of changes in the renal perfusion pressure. The AT2 receptor partially attenuates the proteinuric effects of the AT1 receptor. [ABSTRACT FROM AUTHOR]
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- 2013
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8. Angiotensin receptor-associated proteins: local modulators of the renin-angiotensin system.
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Castrop, Hayo
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KIDNEY secretions , *ANGIOTENSIN receptors , *IMMUNOMODULATORS , *RENIN-angiotensin system , *PROTEIN receptors , *GENE expression - Abstract
The activity of the renin-angiotensin system crucially depends on the rate of renal renin secretion. Changes in renin secretion result in fluctuations of angiotensin II concentrations in the circulation and subsequently in the activation of angiotensin receptors in all accessible target organs. Consequently, various mechanisms have evolved to regulate the local sensitivity to angiotensin II. In this review, an overview of angiotensin II receptor-associated proteins is addressed. These proteins regulate the local sensitivity of receptor-expressing cells by modulating the receptor surface expression and the receptor sensitivity. A hypothesis will be discussed that integrates the existence of various angiotensin receptor-associated proteins into an overall functional model. [ABSTRACT FROM AUTHOR]
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- 2013
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9. Physiology of Kidney Renin.
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Castrop, Hayo, Höcherl, Klaus, Kurtz, Armin, Schweda, Frank, Todorov, Vladimir, and Wagner, Charlotte
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RENIN-angiotensin system , *KIDNEY physiology , *CYTOGENETICS , *GENES , *HOMOLOGY (Biology) , *CYTOLOGY , *SEQUENTIAL analysis - Abstract
The article presents a study which focuses on the physiology of kidney renin and the transcriptional control of the renin gene. The study identifies the organization of the renin gene in various species including mice, sheep and humans and sequential analysis has been performed to examine the interspecies homology in the renin gene structure. The results indicate that the discovery of the gap functions relevance in JG cells has shown novel insights in the cell biology of renin-producing cells.
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- 2010
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10. Genetically modified mice—successes and failures of a widely used technology.
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Castrop, Hayo
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GENETIC engineering , *BIOTECHNOLOGY , *TRANSGENIC organisms , *LABORATORY mice , *TRANSGENES , *GENOMES - Abstract
Genetically modified mice, created by random integration of a transgene into the genome or by targeted mutation of a specific gene, have proven to be extremely powerful tools for studying gene function in vivo. In this article, we give (1) a short overview of the traditional methods in mouse transgenesis and (2) a discussion of the problems with these methods, (3) more recent methods that were developed to overcome these problems, and (4) an outlook on future directions in gene targeting. [ABSTRACT FROM AUTHOR]
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- 2010
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11. Allergen-induced airway hyperresponsiveness is absent in ecto-5′-nucleotidase (CD73)-deficient mice.
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Schreiber, Rainer, Castrop, Hayo, and Kunzelmann, Karl
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ALLERGENS , *LABORATORY mice , *RESPIRATORY organs , *ADENOSINES , *GERM cells - Abstract
Adenosine is formed from extracellular purines by ecto-5′-nucleotidase (CD73) and is an essential player in allergic airway inflammation. The contribution of adenosine and other purines to electrolyte transport and mucociliary clearance was studied in airways of allergen challenged mice. No signs for allergen-induced inflammation were found in CD73−/− mice, and adenosine monophosphate (AMP) was unable to elicit airway Cl− secretion in these animals. Tracheas of ovalbumin (OVA)-treated BALB/c and CD73+/+ mice were hyperresponsive towards methacholine when assessed by Penh and direct optical measurement of contraction. In addition Cl− secretion activated by ATP and ADP was enhanced. These changes were not observed in CD73−/− mice. Expression of CFTR or CLCA was unchanged upon OVA treatment of CD73 mice, suggesting enhanced Cl− secretion due to upregulated purinergic pathways. Mucociliary clearance was determined by measuring particle transport in excised mouse tracheas and was strongly enhanced in OVA-challenged CD73+/+ mice, but remained unchanged in CD73−/− mice. While mucociliary clearance is activated by allergen exposure independent of functional ecto-5′-nucleotidase, airway inflammation is largely dependent on CD73. Thus, ecto-5′-nucleotidase may provide a novel target for therapeutic intervention, probably by local application of ecto-5′-nucleotidase inhibitors through inhalation. [ABSTRACT FROM AUTHOR]
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- 2008
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12. Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and functional significance.
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Castrop, Hayo and Schnermann, Jurgen
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EXONS (Genetics) , *SPLIT genes , *RNA splicing , *CELLS , *RNA - Abstract
The renal Na-K-2Cl cotransporter (NKCC2, BSC1) is selectively expressed in the apical membrane of cells of the thick ascending limb of the loop of Henle (TAL) and macula densa. NKCC2-dependent salt transport constitutes the major apical entry pathway for transepithelial salt reabsorption in the TAL. Although NKCC2 is encoded by a single gene (Slc12a1), differential splicing of the NKCC2 pre-mRNA results in the formation of several alternate transcripts. Thus three full-length splice isoforms of NKCC2 differ in their variable exon 4, resulting in transcripts for NKCC2B, NKCC2A, and NKCC2F. In addition to full-length isoforms, variants with truncated COOH-terminal ends have been described. The various splice isoforms of NKCC2 differ in their localization along the TAL and in their transport characteristics. Data in the literature are reviewed to assess the principles of NKCC2 differential splicing, the localization of NKCC2 splice isoforms along the TAL in various species, and the functional characteristics of the splice isoforms. In addition, we discuss the functional significance of NKCC2 isoforms for TAL salt retrieval and for the specific salt sensor function of macula densa cells based on studies using isoform-specific NKCC2-knockout mice. We suggest that different NKCC2 splice variants cooperate in salt retrieval along the TAL and that the coexpression of two splice variants (NKCC2B and NKCC2A) in the macula densa cells facilitates efficient salt sensing over wide ranges of fluctuating salt concentrations. [ABSTRACT FROM AUTHOR]
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- 2008
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13. Skeletal abnormalities and extra-skeletal ossification in mice with restricted Gsα deletion caused by a renin promoter-Cre transgene.
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Castrop, Hayo, Oppermann, Mona, Mizel, Diane, Yuning Huang, Faulhaber-Walter, Robert, Weiss, Yvonne, Weinstein, Lee S., Min Chen, Germain, Stephane, Huiyan Lu, Ragland, Dan, Schimel, Daniel M., and Schnermann, Jurgen
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SKELETAL abnormalities , *GENOTYPE-environment interaction , *TRANSGENIC mice , *MEMBRANE proteins , *BONE growth - Abstract
We have recently generated a transgenic mouse line (termed hRen-Cre) that expresses Cre-recombinase under the control of a 12.2-kb fragment of the human renin promoter. In the present study, we have crossed hRen-Cre mice with a mouse strain in which exon 1 of the Gnas gene is flanked by loxP sites. Gnas encodes the α-subunit of the stimulatory G protein (Gsα). Our aim has been to generate a mouse model with locally restricted inactivation of Gsα to extend studies of the role of Gsα function in vivo. Mice with local Cre-mediated inactivation of Gsα (rCre-Gsα) are viable and fertile. Their most obvious phenotype consists of marked skeletal malformations of the forelimbs in which computer-tomography scans reveal shortened and fused extremity bones. Extraskeletal ossifications occur in the subcutis and in skeletal muscles associated with the affected long bones. Plasma calcium, phosphate and parathyroid hormone are normal. Skin histology has demonstrated diffuse mineralization and ossification associated with the basal cells of hair follicles. This phenotype in part resembles syndromes in humans associated with loss-of-function of Gsα, such as Albright hereditary osteodystrophy and progressive osseous heteroplasia. The renal phenotype of rCre-Gsα mice is inconspicuous. Plasma renin concentration, ambient urine osmolarity, and the glomerular filtration rate of rCre-Gsα mice do not differ from controls. The absence of measurable functional changes in the renin-angiotensin system indicates insufficient Cre expression in juxtaglomerular granular cells in this strain of mice. Nevertheless, the present report reaffirms the importance of Gsα signaling for bone development and the suppression of ectopic ossification. [ABSTRACT FROM AUTHOR]
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- 2007
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14. Contribution of the basolateral isoform of the Na-K-2Cl- cotransporter (NKCC1/BSC2) to renin secretion.
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Castrop, Hayo, Lorenz, John N., Hansen, Pernille B., Friis, Ulla, Mizel, Diane, Oppermann, Mona, Jensen, Boye L., Briggs, Josie, Skøtt, Ole, and Schnermann, Jurgen
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RENIN , *DIURETICS , *SECRETION , *BIOLOGICAL transport , *MESSENGER RNA , *ASPARTIC proteinases , *FUROSEMIDE , *JUXTAGLOMERULAR apparatus , *MICE - Abstract
Acute administration of loop diuretics like furosemide leads to a stimulation of renin secretion, an effect thought to result from inhibition of Na-K-2Cl cotransporter (NKCC2)-mediated salt transport at the luminal surface of the macula densa (MD). However, loop diuretics also inhibit NKCC1, the second isoform of the Na-K-2Cl cotransporter, with similar potency. In the present study, we examined the influence of furosemide on renin secretion in NKCC1-deficient mice to distinguish between effects of the loop diuretic involving NKCC2 and, by implication, the MD pathway, and effects that might occur via inhibition of NKCC1. Baseline plasma renin concentration (PRC) was 1,212 ± 211 in NKCC1+/+ (n = 13) and 3,851 ± 579 ng ANG I·ml-1·h-1 in NKCC1-/- mice (n = 14; P = 0.00024). Acute administration of furosemide (50 mg/kg i.p.) increased PRC significantly to 9,324 ± 1,018 ng ANG I·ml-1·h-1 in NKCC1+/+ (n = 13; P < 0.0001 compared with basal) and to 14,188 ± 2,274 ng ANG I·m-1·h-1 in NKCC1-/- mice [n = 14; P = 0.0002 compared with basal; P = 0.034 compared with wild-type (WT) plus furosemide]. Renin mRNA expression was about threefold higher in NKCC1-/- compared with WT mice. There was considerable recruitment of granular cells to upstream regions of afferent arterioles in NKCC1-/- mice. Patch-clamp studies in single juxtaglomerular granular (JG) cells from WT mice showed an -10% increase in membrane capacitance during incubation with furosemide (10-4 M), indicating a direct effect of the loop diuretic on renin secretion. No effect of furosemide on membrane capacitance was observed in JG cells from NKCC1-deficient mice. Furosemide (10 M-3) significantly stimulated renin release from primary cultures of JG cells from WT mice, whereas no response was observed in NKCC1-/- mice. Our data suggest that a functional NKCC1 suppresses basal renin release, at least in part, through a direct effect on JG cells. [ABSTRACT FROM AUTHOR]
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- 2005
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15. Impairment of tubuloglomerular feedback regulation of GFR in ecto-5 '-nucleotidase/ CD73--deficient mice.
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Castrop, Hayo, Huang, Yuning, Hashimoto, Seiji, Mizel, Diane, Hansen, Pernille, Theilig, Franziska, Bachmann, Sebastian, Deng, Chuxia, Briggs, Josie, and Schnermann, Jurgen
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ADENOSINES , *IMMUNE response , *GLOMERULAR filtration rate , *ADENOSINE monophosphate , *KIDNEY tubules , *LABORATORY mice - Abstract
Adenosine coordinates organ metabolism and blood supply, and it modulates immune responses. In the kidney it mediates the vascular response elicited by changes in NaCl concentration in the macula densa region of the nephron, thereby serving as an important regulator of GFR. To determine whether adenosine formation depends on extracellular nucleotide hydrolysis, we studied NaCl-dependent GFR regulation (tubuloglomerular feedback) in mice with targeted deletion of ecto-5'-nucleotidase/CD73 (e-5'NT/CD73), the enzyme responsible for adenosine formation from AMP. e-5' NT/CD73-/- mice were viable and showed no gross anatomical abnormalities. Blood pressure, blood and urine chemistry, and renal blood flow were not different between e-5' NT/CD73-/- and e-5' NT/CD73-/- mice. e-5' NT/CD73-/- mice had a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. Furthermore, whereas tubuloglomerular feedback responses did not change significantly during prolonged loop of Henle perfusion in e-5' NT/CD73+/+ mice, a complete disappearance of the residual feedback response was noted in e-5' NT/CD73-/- mice over 10 minutes of perfusion. The contractile response of isolated afferent arterioles to adenosine was normal in e-5' NT/CD73-/- mice. We conclude that the generation of adenosine at the glomerular pole depends to a major extent on e-5' NT/CD73-mediated dephosphorylation of 5'-AMP, presumably generated from released ATP. [ABSTRACT FROM AUTHOR]
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- 2004
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16. Permissive role of nitric oxide in macula densa control of renin secretion.
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Castrop, Hayo, Schwenda, Frank, Mizel, Diane, Huang, Yuning, Briggs, Josie, Kurtz, Armin, and Schnermann, Jurgen
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NITRIC oxide , *RENIN-angiotensin system , *RENIN , *BUMETANIDE , *DIURETICS , *LABORATORY mice - Abstract
Experiments were performed in neuronal (nNOS)- and endothelial nitric oxide synthase (eNOS)-deficient mice to study the role of nitric oxide (NO) in macula densa control of renin secretion in vivo and in the isolated, perfused mouse kidney. Acute and chronic administration of loop diuretics was used as a method to stimulate macula densa-mediated renin secretion. Increases in plasma renin concentration (PRC) in response to a 3-day infusion of bumetanide (50 mg·kg-1·day-1) or an acute injection of furosemide (50 mg/kg ip) were not markedly altered in nNOS-/mice. Responses to furosemide were also maintained in eNOS-/mice, but the administration of Nω-nitro-L-arginine methyl ester (LNAME) markedly attenuated the PRC response to furosemide in these mice. In the isolated kidney preparation, bumetanide caused similar relative increases in renin secretion in kidneys of wild-type, nNOS-/-, and eNOS-/- mice. Bumetanide only marginally increased renin secretion in L-NAME-treated kidneys, but the bumetanide effect was normalized by S-nitroso-N-acetyl-penicillamine. Basal PRC was significantly reduced in male nNOS-/- mice compared with nNOS+/+ (189 ± 28 vs. 355 ± 57 ng ANG I ·ml-1·h-1; P = 0.017). There was no significant difference in PRC between eNOS+/+ and eNOS-/- mice. Basal renin secretion rates in perfused kidneys isolated from nNOS-/- or eNOS-/- mice were markedly reduced compared with wild-type controls. Our data suggest that NO generated by macula densa nNOS does not play a specific mediator role in macula densa-dependent renin secretion. However, NO independent of its exact source permits the macula densa pathway of renin secretion to function normally. [ABSTRACT FROM AUTHOR]
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- 2004
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17. General inhibition of renocortical cyclooxygenase-2 expression by the renin-angiotensin system.
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Castrop, Hayo, Klar, Jürgen, Wagner, Charlotte, Höcherl, Klaus, and Kurtz, Armin
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RENIN-angiotensin system , *CYCLOOXYGENASES , *KIDNEY cortex , *ANGIOTENSIN converting enzyme - Abstract
Because across-the-board data indicate that renin and cyclooxygenase-2 (COX-2) expression in the kidney cortex are regulated in parallel and because ANG II can inhibit COX-2 expression, the purpose of our study was to characterize a potential general inhibitory feedback of the renin-angiotensin system on renocortical COX-2 expression in vivo. Rats were fed a high-, normal-, or low-salt diet or were chronically infused with furosemide (60 mg·kg[sup -1]· day[sup -1]) or the left renal artery was clipped, and the animals were treated in addition to or without the angiotensin-convetting enzyme inhibitor ramipril (10 mg·kg[sup -1]·day[sup -1]). A high-salt diet reduced expression of COX-2, whereas a lowsalt diet, furosemide infusion, and renal artery stenosis stimulated COX-2 expression. Additional angiotensin-converting enzyme inhibition led to further increases in renocortical COX-2 expression by 62, 136, 300, 50, and 70% for a high-, normal-, and low-salt diet, furosemide infusion, and renal artery stenosis, respectively. Thus our data suggest a general inhibitory effect of the renin-angiotensin system on renocortical COX-2 expression. [ABSTRACT FROM AUTHOR]
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- 2003
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18. Nephron specific regulation of chloride channel CLC-K2 mRNA in the rat.
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Vitzthum, Helga, Castrop, Hayo, Meier-Meitinger, Martina, Riegger, Günter A.J, Kurtz, Armin, Krämer, Bernhard K, and Wolf, Konrad
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SALT , *KIDNEY physiology - Abstract
Nephron specific regulation of chloride channel CLC-K2 mRNA in the rat. Background: This study investigated the influence of salt intake on the nephron specific gene expression of the kidney chloride channel CLC-K2. To this end, male Sprague-Dawley rats were fed a low (0.02% wt/wt), normal (0.6% wt/wt), or high salt (8% wt/wt) diet for ten days, or they received the loop diuretic furosemide (12 mg/kg/day) for six days. Methods: Expression and regulation of messenger RNA for CLC-K2 was demonstrated by RNase protection assay and in situ hybridization in kidney cortex, outer medulla and inner medulla. Tubular localization and regulation were determined precisely by reverse transcription-polymerase chain reaction (RT-PCR) and real time PCR of microdissected nephron segments. Results: In situ hybridization analysis and RNase protection assay of the total kidney revealed a down-regulation of CLC-K2 mRNA in the high salt diet rats and an up-regulation of CLC-K2 mRNA in furosemide treated rats, which were restricted to the outer medulla. Microdissection of collagenase treated kidney revealed CLC-K2 mRNA expression in the outer medullary thick ascending limb (mTAL), cortical thick ascending limb (cTAL), distal convoluted tubule (DCT), connecting tubule and cortical collecting duct (CNT/CCD), and outer medullary collecting duct (OMCD), whereas no signals were detected in proximal convoluted and straight tubules (PCT and PST), descending thin limb from the outer medulla (dTL), descending and ascending thin limb from the inner medulla (TL), inner medullary collecting duct (IMCD) and glomeruli (glom). Using RT-PCR and real time PCR, the changing levels of CLC-K2 mRNA after furosemide treatment or high salt diet were restricted to the mTAL, whereas CLC-K2 mRNA levels in cTAL and OMCD were not changed in furosemide or high salt rats compared to time paired controls. Conclusions: Given that CLC-K2 expressed in the thick ascending limb of Henle's loop is responsible for ne... [ABSTRACT FROM AUTHOR]
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- 2002
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19. Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'".
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Castrop, Hayo
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ALBUMINS , *ANGIOTENSIN II , *MICROSCOPY - Abstract
A response from the author of the article "Angiotensin II AT2 receptor activation attenuates AT1 receptor-induced increases in the glomerular filtration of albumin: a multiphoton microscopy study" published in the previous issue of the journal is presented.
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- 2014
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20. Functional evidence confirmed by histological localization: overlapping expression of erythropoietin and HIF-2α in interstitial fibroblasts of the renal cortex.
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Castrop, Hayo and Kurtz, Armin
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ERYTHROPOIETIN , *ERYTHROPOIESIS , *HYPOXEMIA , *TRANSCRIPTION factors , *KIDNEYS - Abstract
Erythropoietin (EPO) is the central hormone in the regulation of erythropoiesis. Synthesis of EPO and its constitutive release into the circulation are controlled by the transcriptional activity of the EPO gene. A key regulator of EPO is the dimeric hypoxia-inducible transcription factor (HIF), which has three isoforms. Paliege and co-workers provide convincing morphological evidence that it is the HIF-2 isoform that essentially triggers EPO in the kidney, which is the primary source of EPO in the body. [ABSTRACT FROM AUTHOR]
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- 2010
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21. Blunted renal autoregulation during high salt intake: advantageous or deleterious?
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Castrop, Hayo
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HIGH-salt diet , *RENAL circulation , *GLOMERULAR filtration rate , *REACTIVE oxygen species , *PHYSIOLOGICAL effects of salt - Abstract
The article discusses mechanisms of renal autoregulation when the salt intake is high by citing the study "High salt diet blunts renal autoregulation by a reactive oxygen species-dependent mechanism" by R.C. Fellner and others, published in this journal. Topics include the effect of a chronic high-salt diet on renal autoregulatory capacity, the formation of reactive oxygen species, and the deleterious impact of reduced renal autoregulation during high salt intake on glomerular capillaries.
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- 2014
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22. Modulation of adenosine receptor expression in the proximal tubule: a novel adaptive mechanism to regulate renal salt and water metabolism.
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Castrop, Hayo
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ADENOSINES , *KIDNEYS , *RATS , *DIET , *METABOLISM - Abstract
The article comments on a study by Kulick and coworkers which provides new insights into the involvement of adenosine in long-term adaptation of the kidney to varying reabsorptive needs. The authors demonstrate that chronic exposure of rats to a salt-restricted diet leads to an increased expression of A1 adenosine receptor (A1-AR) in the proximal tubule, accompanied by an enhanced A1-AR-dependent reabsorptive activity.
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- 2008
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23. Angiotensin II enhances the endocytosis of proteins by podocytes in vivo ő a multiphoton microscopy study (1173.1).
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Schiessl, Ina Maria and Castrop, Hayo
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- 2014
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24. A special issue on the renin-angiotensin system.
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Castrop, Hayo, Kurtz, Armin, and Schweda, Frank
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RENIN-angiotensin system , *ENDOCRINE system , *REGULATION of blood pressure , *ANGIOTENSINOGEN - Published
- 2013
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25. Regulation of the Na+-K+-2Cl− cotransporter by c GMP/c GMP-dependent protein kinase I after furosemide administration.
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Limmer, Franziska, Schinner, Elisabeth, Castrop, Hayo, Vitzthum, Helga, Hofmann, Franz, and Schlossmann, Jens
- Subjects
- *
CGMP-dependent protein kinase , *FUROSEMIDE , *PHOSPHOTRANSFERASES , *LOOP diuretics , *CHEMICAL reactions - Abstract
Sodium chloride reabsorption in the thick ascending limb of the loop of Henle is mediated by the Na+-K+-2Cl− cotransporter ( NKCC2). The loop diuretic furosemide is a potent inhibitor of NKCC2. However, less is known about the mechanism regulating the electrolyte transporter. Considering the well-established effects of nitric oxide on NKCC2 activity, c GMP is likely involved in this regulation. c GMP-dependent protein kinase I (c GKI; PKGI) is a c GMP target protein that phosphorylates different substrates after activation through c GMP. We investigated the potential correlation between the c GMP/c GKI pathway and NKCC2 regulation. We treated wild-type (wt) and c GKIα-rescue mice with furosemide. c GKIα-rescue mice expressed c GKIα only under the control of the smooth muscle-specific transgelin ( SM22) promoter in a c GKI deficient background. Furosemide treatment increased the urine excretion of sodium and chloride in c GKIα-rescue mice compared to that in wt mice. We analyzed the phosphorylation of NKCC2 by western blotting and immunostaining using the phosphospecific antibody R5. The administration of furosemide significantly increased the phosphorylated NKCC2 signal in wt but not in c GKIα-rescue mice. NKCC2 activation led to its phosphorylation and membrane translocation. To examine whether c GKI was involved in this process, we analyzed vasodilator-stimulated phosphoprotein, which is phosphorylated by c GKI. Furosemide injection resulted in increased vasodilator-stimulated phosphoprotein phosphorylation in wt mice. We hypothesize that furosemide administration activated c GKI, leading to NKCC2 phosphorylation and membrane translocation. This c GKI-mediated pathway could be a mechanism to compensate for the inhibitory effect of furosemide on NKCC2. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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26. Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.
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Mederle, Katharina, Meurer, Manuel, Castrop, Hayo, and Höcherl, Klaus
- Subjects
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CYCLOOXYGENASES , *THROMBOXANES , *GLOMERULAR filtration rate , *ENDOTOXEMIA , *LABORATORY mice , *DIAGNOSIS - Abstract
Thromboxane (Tx) A2 has been suggested to be involved in the development of sepsis-induced acute kidney injury (AKI). Therefore, we investigated the impact of cyclooxygenase (COX)-1 and COX-2 activity on lipopolysaccharide (LPS)-induced renal TxA2 formation, and on endotoxemia- induced AKI in mice. Injection of LPS (3 mg/kg ip) decreased glomerular filtration rate (GFR) and the amount of thrombocytes to ~50% of basal values after 4 h. Plasma and renocortical tissue levels of TxB2 were increased ~10- and 1.7-fold in response to LPS, respectively. The COX-1 inhibitor SC-560 attenuated the LPS-induced fall in GFR and in platelet count to ~75% of basal levels. Furthermore, SC-560 abolished the increase in plasma and renocortical tissue levels of TxB2 in response to LPS. The COX-2 inhibitor SC-236 further enhanced the LPS-induced decrease in GFR to 40% of basal values. SC-236 did not alter thrombocyte levels nor the LPS-induced increase in plasma and renocortical tissue levels of TxB2. Pretreatment with clopidogrel inhibited the LPS-induced drop in thrombocyte count, but did not attenuate the LPS-induced decrease in GFR and the increase in plasma TxB2 levels. This study demonstrates that endotoxemia-induced TxA2 formation depends on the activity of COX-1. Our study further indicates that the COX-1 inhibitor SC-560 has a protective effect on the decrease in renal function in response to endotoxin. Therefore, our data support a role for TxA2 in the development of AKI in response to LPS. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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27. Superficial Nephrons in BALB/c and C57BL/6 Mice Facilitate In VivoMultiphoton Microscopy of the Kidney.
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Schießl, Ina Maria, Bardehle, Sophia, and Castrop, Hayo
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MICROSCOPY , *ORGANS (Anatomy) , *LASERS , *KIDNEY glomerulus , *KIDNEY cortex , *KIDNEYS - Abstract
Multiphoton microscopy (MPM) offers a unique approach for addressing both the function and structure of an organ in near-real time in the live animal. The method however is limited by the tissue-specific penetration depth of the excitation laser. In the kidney, structures in the range of 100 µ from the surface are accessible for MPM. This limitation of MPM aggravates the investigation of the function of structures located deeper in the renal cortex, like the glomerulus and the juxtaglomerular apparatus. In view of the relevance of gene-targeted mice for investigating the function of these structures, we aimed to identify a mouse strain with a high percentage of superficially located glomeruli. The mean distance of the 30 most superficial glomeruli from the kidney surface was determined in 10 coµonly used mouse strains. The mean depth of glomeruli was 118.4±e.4, 123.0±2.7, 133.7±3.0, 132.3±2.6, 141.0±4.0, 145.3±4.3, 148.9±4.2, 151.6±2.7, 167.7±3.9, and 207.8±3.2 µ in kidney sections from 4-week-old C3H/HeN, BALB/cAnN, SJL/J, C57BL/6N, DBA/2N, CD1 (CRI), 129S2/SvPas, CB6F1, FVB/N and NMRI (Han) mice, respectively (n = 5 animals from each strain). The mean distance from the kidney surface of the most superficial glomeruli was significantly lower in the strains C3H/HeN Crl, BALB/cAnN, DBA/2NCrl, and C57BL/6N when compared to a peer group consisting of all the other strains (p,.0001). In 10-week-old mice, the most superficial glomeruli were located deeper in the cortex when compared to 4-week-old animals, with BALB/cAnN and C57BL/6N being the strains with the highest percentage of superficial glomeruli (25% percentile 116.7 and 121.9 µ, respectively). In suµary, due to significantly more superficial glomeruli compared to other coµonly used strains, BALB/cAnN and C57BL/ 6N mice appear to be particularly suitable for the investigation of glomerular function using MPM. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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28. Intrarenal Renin Angiotensin System Revisited.
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Pohl, Marcus, Kaminski, Henriette, Castrop, Hayo, Bader, Michael, Himmerkus, Nina, Bleich, Markus, Bachmann, Sebastian, and Theilig, Franziska
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BIOSYNTHESIS , *BIOCHEMICAL engineering , *ABSORPTION (Physiology) , *CELL culture , *CELL lines - Abstract
The existence of a local renin angiotensin system (RAS) of the kidney has been established. Angiotensinogen (AGT), reni angiotensin-converting enzyme (ACE), angiotensin recep- tors, and high concentrations of luminal angiotensin II have been found in the proximal tubule. Although functional data have documented the relevance of a local RAS, the dualism between biosynthesis and endocytotic uptake of its compo- nents and their cellular processing has been incompletely un- derstood. To resolve this, we have selectively analyzed their distribution, endocytosis, transcytosis, and biosynthesis in the proximal tubule. The presence of immunoreactive AGT, restricted to the early proximal tubule, was due to its retrieval from the ultrafiltrate and storage in endosomal and lysosomal compartments. Cellular uptake was demonstrated by autora- diography of radiolabeled AGT and depended on intact endocytosis. AGT was identified as a ligand of the multiple ligand- binding repeats of megalin. AGT biosynthesis was restricted to the proximal straight tubule, revealing substantial AGT mRNA expression. Transgenic AGT overexpression under the control of an endogenous promoter was also restricted to the late proximal tubule. Proximal handling of renin largely followed the patterns of AGT, whereas its local biosynthesis was not significant. Transcytotic transport of AGT in a proximal cell line revealed a 5% recovery rate after 1 h. ACE was expressed along late proximal brush-border membrane, whereas ACE2 was present along the entire segment. Surface expression of ACE and ACE2 differed as a function of endocytosis. Our data on the localization and cellular processing of RAS components provide new aspects of the functional concept of a "self-con- tained" renal RAS. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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29. Vasodilatation of afferent arterioles and paradoxical increase of renal vascular resistance by furosemide in mice.
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Oppermann, Mona, Hansen, Pernille B., Castrop, Hayo, and Schnermann, Jurgen
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FUROSEMIDE , *ANGIOTENSINS , *DIURETICS , *HEMODYNAMICS , *BLOOD circulation , *AMINO acids , *ARGININE , *INDOMETHACIN - Abstract
Loop diuretics like furosemide have been shown to cause renal vasodilatation in dogs and humans, an effect thought to result from both a direct vascular dilator effect and from inhibition of tubuloglomerular feedback. In isolated perfused afferent arterioles preconstricted with angiotensin II or NG-nitro-L-arginine methyl ester, furosemide caused a dose-dependent increase of vascular diameter, but it was without effect in vessels from NKCCl-/- mice suggesting that inhibition of NKCCl mediates dilatation in afferent arterioles. In the intact kidney, however, furosemide (2 mg/kg iv) caused a 50.5 ± 3% reduction of total renal blood flow (RBF) and a 27% reduction of superficial blood flow (SBF) accompanied by a marked and immediate increase of tubular pressure and volume. At 10 mg/kg, furosemide reduced RBF by 60.4 ± 2%. Similarly, NKCCl-/- mice responded to furosemide with a 45.4% decrease of RBF and a 29% decrease of SBF. Decreases in RBF and SBF and increases of tubular pressure by furosemide were ameliorated by renal decapsulation. In addition, pretreatment with candesartan (2 mg/kg) or indomethacin (5 mg/kg) attenuated the reduction of RBF and peak urine flows caused by furosemide. Our data indicate that furosemide, despite its direct vasodilator potential in isolated afferent arterioles, causes a marked increase in flow resistance of the vascular bed of the intact mouse kidney. We suggest that generation of angiotensin II and/or a vasoconstrictor prostaglandin combined with compression of peritubular capillaries by the expanding tubular compartment are responsible for the reduction of RBF in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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30. Effect of carbonic anhydrase inhibition on GFR and renal hemodynamics in adenosine-1 receptor-deficient mice.
- Author
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Hashimoto, Seiji, Huang, Yuning G., Castrop, Hayo, Hansen, Pernille B., Mizel, Diane, Briggs, Josie, and Schnermann, Jurgen
- Subjects
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GLOMERULAR filtration rate , *KIDNEY function tests , *KIDNEY glomerulus , *RENAL tubular transport , *ANGIOTENSINS , *HEMODYNAMICS , *RENIN - Abstract
The reduction of glomerular filtration rate (GFR) caused by inhibitors of carbonic anhydrase (CA) is thought to be initiated by activation of the tubuloglomerular feedback (TGF) mechanism. We determined the effect of the CA inhibitor benzolamide (Bz) on renal hemodynamics in adenosine-1 receptor (A1AR) knockout mice that have been shown previously to lack a TGF response. InA1AR+/+ mice, Bz (150 µg plus 2 µg/min) reduced RBF by 19.8% (from 829±42 to 666±44 µl/min;n=7), and GFR by 19.8% (from 396±43 to 324±46 µl/min;n=9,P=0.001). InA1AR-/- mice, RBF fell by 15.9 % (from 809±24 to 680±40 µl/min;n=7), and GFR by 21.1% (from 358±27 to 287±32 µl/min;n=10,P=0.0003; NS compared withA1AR+/+). The absence of TGF responses both before and during Bz infusion inA1AR-/- mice was confirmed by micropuncture. Following angiotensin II-receptor blockade with candesartan, Bz did not alter RBF (1.4±0.2 vs. 1.4±0.15 ml/min inA1AR+/+, and 1.4±0.22 vs. 1.39±0.2 ml/min inA1AR-/-;n=5/genotype) while GFR changed by -8.9 % inA1AR+/+ mice (n=7), and by -1% inA1AR-/- mice (n=9; NS compared withA1AR+/+). Bz caused a significant rise of plasma renin concentration in bothA1AR+/+ andA1AR-/- mice. Our data show that the absence of a functional TGF mechanism does not prevent the reduction in GFR or RBF caused by CA inhibition. Acute angiotensin II receptor blockade, on the other hand, diminishes the effect of CA inhibition on GFR and RBF. The causes for the GFR reduction appear to be complex and include an effect of the renin-angiotensin system. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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31. Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice.
- Author
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Wagner, Charlotte, Vitzthum, Helga, Castrop, Hayo, Schumacher, Karl, Bucher, Michael, Albertin, Sybille, Coffman, Thomas M., Arendshorst, William J., and Kurtz, Armin
- Subjects
- *
RENIN , *ASPARTIC proteinases , *JUXTAGLOMERULAR apparatus , *KIDNEY cortex , *KIDNEYS , *MICE - Abstract
Based on the controversy about the relevance of cyclooxygenase-2 (Cox-2)-derived prostanoids from the macula densa for the control of the renin system, this study aimed to determine the interrelation between Cox-2 and renin expression in the mouse kidney. In control mice renin mRNA was readily detectable whilst renocortical Cox-2 mRNA abundance was at the detection limit of the RNase protection assay and no specific signals for Cox-2 were obtained by in situ hybridization or Western blot analysis. Experimental maneuvers such as low-salt diet, treatment with loop diuretics or angiotensin I converting enzyme inhibitors clearly increased renin mRNA abundance up to sevenfold, but under none of these conditions renocortical Cox-2 mRNA levels were significantly changed. Moreover, the strong stimulation of renin expression by angiotensin I-converting enzyme inhibition was not changed by the cyclooxygenase inhibitor ibuprofen, which in turn clearly lowered tissue prostanoid content. Our data suggest a marked divergence of renin and Cox-2 expression in the kidney cortex of C57Bl/6 mice with no clear evidence for a role of Cox-2-derived prostanoids from the macula densa in the regulation of renin expression. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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32. Mesangial cells regulate the single nephron GFR and preserve the integrity of the glomerular filtration barrier: An intravital multiphoton microscopy study.
- Author
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Ziegler, Vera, Fremter, Katharina, Helmchen, Julia, Witzgall, Ralph, and Castrop, Hayo
- Subjects
- *
KIDNEY tubules , *ANGIOTENSIN II , *BASAL lamina , *GLOMERULAR filtration rate , *LABORATORY rats - Abstract
Aim: The intraglomerular mesangial cells are located between the glomerular capillaries. Here we hypothesized that mesangial cells regulate the single nephron glomerular filtration rate (snGFR) and that mesangial cells support the integrity of the glomerular filtration barrier. Methods: We assessed the function of mesangial cells in vivo by multiphoton microscopy. Mesangial cells were depleted in Munich Wistar Froemter rats using the Thy1.1 antibody model. Results: The Thy1.1 antibody caused the cell‐specific loss of 82 ± 3% of mesangial cells. After mesangial cell depletion, the baseline snGFR was reduced to 12.0 ± 1.2 vs 32.4 ± 3.2 nL/min in controls. In control rats, the snGFR decreased after angiotensin II infusion by 61 ± 3% (P =.004), whereas it remained unchanged in Thy1.1‐treated rats. The changes in the snGFR after angiotensin II infusion in control rats were accompanied by the marked rotation of the capillary loops within Bowman's space. This phenomenon was absent in anti‐Thy1.1‐treated rats. The glomerular sieving coefficient (GSCA) for albumin, used as a measure of the integrity of the glomerular filtration barrier, was low in control rats (0.00061 ± 0.00004) and increased after angiotensin II infusion (0.00121 ± 0.00015). In Thy1.1‐treated rats, the GSC was elevated (0.0032 ± 0.00059) and did not change in response to angiotensin II. Electron microscopy revealed the increased thickness of the glomerular basement membrane after mesangial cell depletion. Conclusion: Our data suggest that mesangial cells actively contribute to the regulation of the snGFR. Furthermore, mesangial cells are crucially involved in maintaining the integrity of the glomerular filtration barrier, in part by modulating the thickness of the glomerular basement membrane. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
33. Angiotensin-Receptor-Associated Protein Modulates Ca2+ Signals in Photoreceptor and Mossy Fiber cells.
- Author
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Barro-Soria, Rene, Caicedo, Alejandro, Jägle, Herbert, Merkel, Laura, Zhao, Na, Knop, Gabriel, Gierke, Kaspar, Dannullis, Andrea, Castrop, Hayo, Brandstätter, Johann Helmut, Kirchhoff, Frank, Feigenspan, Andreas, and Strauß, Olaf
- Subjects
- *
ANGIOTENSINS , *PHOTORECEPTORS , *NEUROTRANSMITTERS , *CENTRAL nervous system , *CEREBELLUM - Abstract
Fast, precise and sustained neurotransmission requires graded Ca2+ signals at the presynaptic terminal. Neurotransmitter release depends on a complex interplay of Ca2+ fluxes and Ca2+ buffering in the presynaptic terminal that is not fully understood. Here, we show that the angiotensin-receptor-associated protein (ATRAP) localizes to synaptic terminals throughout the central nervous system. In the retinal photoreceptor synapse and the cerebellar mossy fiber-granule cell synapse, we find that ATRAP is involved in the generation of depolarization-evoked synaptic Ca2+ transients. Compared to wild type, Ca2+ imaging in acutely isolated preparations of the retina and the cerebellum from ATRAP knockout mice reveals a significant reduction of the sarcoendoplasmic reticulum (SR) Ca2+-ATPase (SERCA) activity. Thus, in addition to its conventional role in angiotensin signaling, ATRAP also modulates presynaptic Ca2+ signaling within the central nervous system. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
34. Impaired glucose tolerance, glucagon, and insulin responses in mice lacking the loop diuretic-sensitive Nkcc2a transporter.
- Author
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Kelly, Lisa, Almutairi, Mohammed M., Kursan, Shams, Pacheco, Romario, Dias-Junior, Eduardo, Castrop, Hayo, and Fulvio, Mauricio Di
- Abstract
The Na K2Cl cotransporter-2 (Nkcc2, Slc12a1) is abundantly expressed in the kidney and its inhibition with the loop-diuretics bumetanide and furosemide has been linked to transient or permanent hyperglycemia in mice and humans. Notably, Slc12a1 is expressed at low levels in hypothalamic neurons and in insulin-secreting β-cells of the endocrine pancreas. The present study was designed to determine if global elimination of one of the Slc12a1 products, i.e., Nkcc2 variant a (Nkcc2a), the main splice version of Nkcc2 found in insulin-secreting β-cells, has an impact on the insulin and glucagon secretory responses and fuel homeostasis in vivo. We have used dynamic tests of glucose homeostasis in wild-type mice and mice lacking both alleles of Nkcc2a (Nkcc2aKO) and assessed their islet secretory responses in vitro. Under basal conditions, Nkcc2aKO mice have impaired glucose homeostasis characterized by increased blood glucose, intolerance to the sugar, delayed/blunted in vivo insulin and glucagon responses to glucose, and increased glycemic responses to the gluconeogenic substrate alanine. Further, we provide evidence of conserved quantitative secretory responses of Nkcc2aKO islets within a context of increased islet size related to hyperplastic/hypertrophic glucagon- and insulin-positive cells (α-cells and β-cells, respectively), normal total islet Cl- content, and reduced β-cell expression of the Cl- extruder Kcc2. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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35. A highly efficient strategy to determine genotypes of genetically-engineered mice using genomic DNA purified from hair roots.
- Author
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Otaño-Rivera, Víctor, Boakye, Amma, Grobe, Nadja, Almutairi, Mohammed M., Kursan, Shams, Mattis, Lesan K., Castrop, Hayo, Gurley, Susan B., Elased, Khalid M., Boivin, Gregory P., and Di Fulvio, Mauricio
- Subjects
- *
MICE breeding , *GENOTYPE-environment interaction , *NUCLEOTIDE sequence , *GENETIC markers , *POLYMERASE chain reaction - Abstract
Genotyping of genetically-engineered mice is necessary for the effective design of breeding strategies and identification of mutant mice. This process relies on the identification of DNA markers introduced into genomic sequences of mice, a task usually performed using the polymerase chain reaction (PCR). Clearly, the limiting step in genotyping is isolating pure genomic DNA. Isolation of mouse DNA for genotyping typically involves painful procedures such as tail snip, digit removal, or ear punch. Although the harvesting of hair has previously been proposed as a source of genomic DNA, there has been a perceived complication and reluctance to use this non-painful technique because of low DNA yields and fear of contamination. In this study we developed a simple, economic, and efficient strategy using Chelex® resins to purify genomic DNA from hair roots of mice which are suitable for genotyping. Upon comparison with standard DNA purification methods using a commercially available kit, we demonstrate that Chelex® efficiently and consistently purifies high-quality DNA from hair roots, minimizing pain, shortening time and reducing costs associated with the determination of accurate genotypes. Therefore, the use of hair roots combined with Chelex® is a reliable and more humane alternative for DNA genotyping. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
36. Profound hypothermia after adenosine kinase inhibition in A1AR-deficient mice suggests a receptor-independent effect of intracellular adenosine.
- Author
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Eisner, Christoph, Kim, SooMi, Grill, Alexandra, Qin, Yan, Hoerl, Marion, Briggs, Josephine, Castrop, Hayo, Thiel, Manfred, and Schnermann, Jurgen
- Subjects
- *
HYPOTHERMIA , *ADENOSINE kinase , *NUCLEOSIDES , *PHOSPHORYLATION , *LABORATORY mice - Abstract
Administration of the nucleoside adenosine has been shown to induce hypothermia in a number of species, an effect mediated predominantly by the adenosine 1 receptor (A1AR) subtype. The present experiments were performed to explore the possibility that the rise of intracellular adenosine levels expected to accompany adenosine administration may contribute to the hypothermic effect of adenosine independent of A1AR activation. Since phosphorylation of adenosine by adenosine kinase (ADK) is causal in the maintenance of low intracellular adenosine, we have examined the effect of ADK inhibition on core body temperature (CBT). Our data show that inhibition of ADK by A-134974 causes a long-lasting deep hypothermia in wild-type mice. Since there was an about 4-fold increase of adenosine plasma levels, experiments were repeated in A1AR−/− mice. ADK inhibition caused deep hypothermia despite the absence of A1AR, although the effect was significantly reduced compared to WT. Furthermore, the dose-dependent hypothermia caused by adenosine administration in WT mice was found to be reduced, but not abolished in A1AR−/− mice. To assess the possible role of A2AR and A3AR activation in our experimental setting, we compared the effects of the agonists CPA (A1AR), CGS21680 (A2AR), and IB-MECA (A3AR) on CBT. Hypothermia induced by CPA was much greater than that caused by CGS21680 or IB-MECA indicating that A1AR activation is the major receptor-dependent pathway for adenosine-induced hypothermia under our experimental conditions. Induction of deep hypothermia by inhibition of ADK, maintenance of this effect in A1AR−/− mice, and maintenance of adenosine-induced hypothermia in A1AR-deficient mice suggest that a receptor-independent action of adenosine requiring intact function of adenosine kinase contributes importantly to the hypothermia induced by adenosine. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
37. The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+ -ATPase SERCA2a.
- Author
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Mederle, Katharina, Gess, Bernhard, Pluteanu, Florentina, Plackic, Jelena, Tiefenbach, Klaus-Jürgen, Grill, Alexandra, Kockskämper, Jens, and Castrop, Hayo
- Subjects
- *
ANGIOTENSIN receptors , *ADENOSINE triphosphatase , *IMMUNOPRECIPITATION , *SURFACE plasmon resonance , *SPECTROMETRY , *VESICLES (Cytology) - Abstract
Aims: The angiotensin II type 1 receptor-associated protein (Atrap) is highly expressed in the heart, but its function in the heart is unknown. We hypothesized that cardiac Atrap may interact with proteins other than the A T I receptor. Methods and results: To identify potential novel interacting partners of Atrap, pull-down assays were performed. Sequencing by MALDI-MS and results of the isolated complexes showed that Atrap interacts with the cardiac Ca2+-ATPase SERCA2a. The interaction between Atrap and SERCA2a was confirmed by co-immunoprecipitation and by surface plasmon resonance (SPR) spectroscopy. Atrap enhanced the SERCA-dependent Ca2+ uptake in isolated SR membrane vesicles. Furthermore, sarcomere shortenings and [Ca2+]i transients (CaTs) were determined in ventricular myocytes isolated from Atrap--/-- and wild-type (WT) mice. The amplitudes of CaTs and sarcomere shortenings were similar in Atrap--/-- and WT myocytes. However, the CaT decay and sarcomere re-lengthening were prolonged in Atrap--/-- myocytes. To further evaluate the functional relevance of the Atrap-SERCA2a interaction in vivo, left-ventricular function was assessed in WT and Atrap--/-- mice. The heart rates (564 ± 10 b.p.m. vs. 560 ± 11 b.p.m.; P = 0.80) and ejection fractions (71.3 ± 1.3 vs. 72 ± 1.8%; P = 0.79) were similar in WT and Atrap--/-- mice, respectively (n = 15 for each genotype). However, the maximum filling rate (dV/dtmax) was markedly decreased in Atrap--/-- (725 ± 48 μL/s) compared with WT mice (1065 ± 122 μL/s; P = 0.01; n = 15). Conclusion: We identified Atrap as a novel regulatory protein of the cardiac Ca2+-ATPase SERCA2a. We suggest that Atrap enhances the activity of SERCA2a and, consequently, facilitates ventricular relaxation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
38. Atrap interacts with the cardiac Ca2+‐ATPase SERCA2A and facilitates ventricular relaxation (1150.7).
- Author
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Mederle, Katharina, Gess, Bernhard, and Castrop, Hayo
- Published
- 2014
- Full Text
- View/download PDF
39. Dietary salt intake modulates differential splicing of the Na-K-2Cl cotransporter NKCC2.
- Author
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Maria Schieβl, Ina, Rosenauer, Agnes, Kattler, Veronika, Minuth, Will W., Oppermann, Mona, and Castrop, Hayo
- Subjects
- *
SALT content of food , *SODIUM-potassium-chloride cotransporters , *EXTREMITIES (Anatomy) , *GENETIC engineering , *LOOP of Henle , *LABORATORY mice - Abstract
Schieβl IM, Rosenauer A, Kattler V, Minuth WW, Oppermann M, Castrop H. Dietary salt intake modulates differential splicing of the Na-K-2Cl cotransporter NKCC2. Am J Physiol Renal Physiol 305: F1139-F1148, 2013. First published August 14, 2013; doi:10.1152/ajprenal.00259.2013.--Both sodium reabsorption in the thick ascending limb of the loop of Henle (TAL) and macula densa salt sensing crucially depend on the function of the Na/K/2Cl cotransporter NKCC2. The NKCC2 gene gives rise to at least three different full-length NKCC2 isoforms derived from differential splicing. In the present study, we addressed the influence of dietary salt intake on the differential splicing of NKCC2. Mice were subjected to diets with low-salt, standard salt, and high-salt content for 7 days, and NKCC2 isoform mRNA abundance was determined. With decreasing salt intake, we found a reduced abundance of the low-affinity isoform NKCC2A and an increase in the high-affinity isoform NKCC2B in the renal cortex and the outer stripe of the outer medulla. This shift from NKCC2A to NKCC2B during a low-salt diet could be mimicked by furosemide in vivo and in cultured kidney slices. Furthermore, the changes in NKCC2 isoform abundance during a salt-restricted diet were partly mediated by the actions of angiotensin II on AT1 receptors, as determined using chronic angiotensin II infusion. In contrast to changes in oral salt intake, water restriction (48 h) and water loading (8% sucrose solution) increased and suppressed the expression of all NKCC2 isoforms, without changing the distribution pattern of the single isoforms. In summary, the differential splicing of NKCC2 pre-mRNA is modulated by dietary salt intake, which may be mediated by changes in intracellular ion composition. Differential splicing of NKCC2 appears to contribute to the adaptive capacity of the kidney to cope with changes in reabsorptive needs. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
40. Loss of WNK3 is compensated for by the WNK1/SPAK axis in the kidney of the mouse.
- Author
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Mederle, Katharina, Mutig, Kerim, Paliege, Alexander, Carota, Isabel, Bachmann, Sebastian, Castrop, Hayo, and Oppermann, Mona
- Abstract
WNK3 kinase is expressed throughout the nephron and acts as a positive regulator of NKCC2 and NCC in vitro. Here we addressed the in vivo relevance of WNK3 using WNK3-deficient mice. WNK3-/- mice were viable and showed no gross abnormalities. The net tubular function was similar in wild-type (WT) and WNK3-/- mice as assessed by determination of 24-h urine output (1.63 ± .06 in WT and 1.55 ± .1 ml in WNK3-/-, n=16; P=0.42) and ambient urine osmolarity (1,804 ± 62 in WT vs. 1,819 ± 61 mosmol/kg in WNK3-/-, n=40; P=0.86). Water restriction (48 h) increased urine osmolarity similarly in both genotypes to 3,440 ± 220 and 3,200 ± 180 mosmol/kg in WT and WNK3-/- mice, respectively (n=11; P=0.41). The glomerular filtration rate (343 ± 22 vs. 315 ± 13 ml/min), renal blood flow (1.35 ± 0.1 vs. 1.42 ± 0.04 ml), and plasma renin concentration (94 ± 18 vs. 80 ± 13 ng ANG I·ml(-1)·h(-1)) were similar between WT and WNK3-/- mice (n=13; P=0.54). WNK1 was markedly upregulated in WNK3-deficient mice, whereas the expression of WNK4 was similar in both genotypes. When the mice were fed a salt-restricted diet [0.02% NaCl (wt/wt)] the levels of pSPAK/OSR1, pNKCC2, and pNCC were enhanced in both genotypes compared with the baseline conditions, with the levels in WNK3-/- exceeding those in WT mice. The upregulation of pSPAK/OSR1, pNKCC2, and pNCC in WNK3-/- mice relative to the levels in WT mice when fed a low-salt diet was paralleled by an increased diuresis in response to hydrochlorothiazide. In summary, the overall relevance of WNK3 for the renal reabsorption of NaCl appears to be limited and can be largely compensated for by the activation of WNK3-independent pathways. Consequently, our data suggest that WNK3 may serve as a member of a kinase network that facilitates the fine-tuning of renal transepithelial NaCl transport. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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41. Direct assessment of tubuloglomerular feedback responsiveness in connexin 40-deficient mice.
- Author
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Oppermann, Mona, Carota, Isabel, Schiessl, Ina, Eisner, Christoph, Castrop, Hayo, and Schnermann, Jurgen
- Abstract
Participation of connexin 40 (Cx40) in the regulation of renin secretion and in the tubuloglomerular feedback (TGF) component of renal autoregulation suggests that gap junctional coupling through Cx40 contributes to the function of the juxtaglomerular apparatus. In the present experiments, we determined the effect of targeted Cx40 deletion in C57BL/6 and FVB mice on TGF responsiveness. In C57BL/6 mice, stop-flow pressure (PSF) fell from 40.3 ± 2 to 34.5 ± 2 mmHg in wild-type (WT) and from 31 ± 1.06 to 26.6 ± 0.98 mmHg in Cx40-/- mice. PSF changes of 5.85 ± 0.67 mmHg in WT and of 4.3 ± 0.55 mmHg in Cx40-/- mice were not significantly different (P = 0.08). In FVB mice, PSF fell from 37.4 ± 1.5 to 31.6 ± 1.5 mmHg in WT and from 28.1 ± 1.6 to 25.4 ± 1.7 mmHg in Cx40-/-, with mean TGF responses being significantly greater in WT than Cx40-/- (5.5 ± 0.55 vs. 2.7 ± 0.84 mmHg; P = 0.002). In both genetic backgrounds, PSF values were significantly lower in Cx40 than WT mice at all flow rates. Arterial blood pressure in the animals prepared for micropuncture was not different between WT and Cx40-/- mice. We conclude that the TGF response magnitude in superficial cortical nephrons is reduced by 30-50% in mice without Cx40, but that with the exception of a small number of nephrons, residual TGF activity is maintained. Thus gap junctional coupling appears to modulate TGF, perhaps by determining the kinetics of signal transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
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42. Angiotensin-2-Mediated Ca2+ Signaling in the Retinal Pigment Epithelium: Role of Angiotensin-Receptor-Associated-Protein and TRPV2 Channel.
- Author
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Barro-Soria, Rene, Stindl, Julia, Müller, Claudia, Foeckler, Renate, Todorov, Vladimir, Castrop, Hayo, and Strauß, Olaf
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HIV infections , *SEX workers , *PUBLIC health research , *CONDOM use , *HEALTH Belief Model - Abstract
Angiotensin II (AngII) receptor (ATR) is involved in pathologic local events such as neovascularisation and inflammation including in the brain and retina. The retinal pigment epithelium (RPE) expresses ATR in its AT1R form, angiotensin-receptor-associated protein (Atrap), and transient-receptor-potential channel-V2 (TRPV2). AT1R and Atrap co-localize to the basolateral membrane of the RPE, as shown by immunostaining. Stimulation of porcine RPE (pRPE) cells by AngII results in biphasic increases in intracellular free Ca2+ inhibited by losartan. Xestospongin C (xest C) and U-73122, blockers of IP3R and PLC respectively, reduced AngII-evoked Ca2+ response. RPE cells from Atrap-/- mice showed smaller AngII-evoked Ca2+ peak (by 22%) and loss of sustained Ca2+ elevation compared to wild-type. The TRPV channel activator cannabidiol (CBD) at 15 µM stimulates intracellular Ca2+-rise suggesting that porcine RPE cells express TRPV2 channels. Further evidence supporting the functional expression of TRPV2 channels comes from experiments in which 100 µM SKF96365 (a TRPV channel inhibitor) reduced the cannabidiol-induced Ca2+ -rise. Application of SKF96365 or reduction of TRPV2 expression by siRNA reduced the sustained phase of AngII-mediated Ca2+ transients by 53%. Thus systemic AngII, an effector of the local renin-angiotensin system stimulates biphasic Ca2+ transients in the RPE by releasing Ca2+ from cytosolic IP3-dependent stores and activating ATR/Atrap and TRPV2 channels to generate a sustained Ca2+ elevation. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
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43. Angiotensin AT1 receptor-associated protein Arap1 in the kidney vasculature is suppressed by angiotensin II.
- Author
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Doblinger, Elisabeth, Höcherl, Klaus, Mederle, Katharina, Kattler, Veronika, Walter, Steen, Hansen, Pernille B., Jensen, Boye, and Castrop, Hayo
- Abstract
Arap1 is a protein that interacts with angiotensin II type 1 (AT1) receptors and facilitates increased AT1 receptor surface expression in vitro. In the present study, we assessed the tissue localization and regulation of Arap1 in vivo. Arap1 was found in various mouse organs, with the highest expression in the heart, kidney, aorta, and adrenal gland. Renal Arap1 protein was restricted to the vasculature and to glomerular mesangial cells and was absent from tubular epithelia. A similar localization was found in human kidneys. To test the hypothesis that angiotensin II may control renal Arap1 expression, mice were subjected to various conditions to alter the activity of the renin-angiotensin system. A high-salt diet (4% NaCl, 7 days) upregulated Arap1 expression in mice by 47% compared with controls (0.6% NaCl, P = 0.03). Renal artery stenosis (7 days) or water restriction (48 h) suppressed Arap1 levels compared with controls (-64 and -62% in the clipped and contralateral kidney, respectively; and -50% after water restriction, P < 0.01). Angiotensin II infusion (2 μgkg-1min-1, 7 days) reduced Arap1 mRNA levels compared with vehicle by 29% (P < 0.01), whereas AT1 antagonism (losartan, 30 mgkg-1day-1, 7 days) enhanced Arap1 mRNA expression by 52% (P < 0.01); changes in mRNA were paralleled by Arap1 protein abundance. Experiments with hydralazine and epithelial nitric oxide synthase-/- mice further suggested that Arap1 expression changed in parallel with angiotensin II, rather than with blood pressure per se. Similar to in vivo, Arap1 mRNA and protein were suppressed by angiotensin II in a time- and dose-dependent manner in cultured mesangial cells. In summary, Arap1 is highly expressed in the renal vasculature, and its expression is suppressed by angiotensin II. Thus Arap1 may serve as a local modulator of vascular AT1 receptor function in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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44. Glomerular hypertension and hyperfiltration in young fvb.ROP Os/+ mice.
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Oppermann, Mona, Faulhaber-Walter, Robert, Castrop, Hayo, Mizel, Diane, Heilig, Kathleen O., Heilig, Charles W., and Schnermann, Jurgen
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RENAL hypertension , *KIDNEY glomerulus diseases , *GLOMERULAR filtration rate , *KIDNEY tubules , *KIDNEY diseases , *LABORATORY mice - Abstract
ROP Os+/- mice, generated by a radiation-induced 10 Mb DNA inversion of chromosome 8 and resulting in an oligosyndactyly (Os) allele, have a 50% reduction in nephron number and develop glomerulosclerosis without altered survival. In contrast, Os+/- mice on an fvb background (fvb.ROP Os/+) die around 3 months of age of rapidly progressing renal failure. Here we assessed renal function in 4-5 wk old Os/+ and wild type (WT) mice. Kidneys of Os/+ were smaller than WT (135 ± 8 mg vs. 295 ± 20 mg; p<.001) whereas body weight did not differ. GFR determined by FITC-inulin clearance in conscious mice was reduced in Os/+ vs. WT (80 ± 10 vs. 258 ± 21 µl/min, p<.001). Glomerular counts per kidney averaged 1461 ± 163 in Os/+ and 10021 ± 480 in WT (p<.001). Calculated SNGFR was 26 ± 4.6 in Os/+ and 12.6 ± 1.4 nl/min in WT (p=.006), and this correlated with increased stop flow (49.8 ± 1.7 vs. 39.6 ± 1.5 mm Hg; p<0.001) and free flow pressures (17.2 ± 1.1 vs.14.5 ± 0.7 mm Hg, p<0.05). Proximal and distal tubular flow rates were elevated in Os/+ mice while distal Cl concentration was normal. BUN was elevated in Os/+ mice compared to WT (93 ± 8.8 vs. 29 ± 3.5 mg/dl; p<0.01). Ambient urinary osmolarity averaged 748 ± 29 mOsm in Os/+ and 1179 ± 118 mOsm in WT (p=.015). Plasma renin was reduced in Os/+ vs. WT (318 ± 59 vs. 807 ± 86 ng Ang I/ml/hr; p=.001) as was renin mRNA (21 ± 3% of WT normalized by 18s RNA; p<.001). In summary, Os/+ mice have an 85% reduction of nephron number, and this is associated with glomerular hypertension, hyperfiltration and hypertrophy of existing nephrons, likely causes of the progressive deterioration of renal function. [ABSTRACT FROM AUTHOR]
- Published
- 2007
45. cAMP target sequences enhCRE and CNRE sense low-salt intake to increase human renin gene expression in vivo.
- Author
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Desch, Michael, Harlander, Sabine, Neubauer, Björn, Gerl, Melanie, Germain, Stephane, Castrop, Hayo, and Todorov, Vladimir
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ADENOSINE monophosphate , *GENE expression , *RENIN , *TRANSGENIC mice , *GENETIC mutation , *ADRENERGIC receptors - Abstract
This study aimed to assess the role of cAMP target sequences enhancer cAMP response element (enhCRE) and cAMP and overlapping negative response element (CNRE) in the control of human renin gene (REN) in vivo. enhCRE and CNRE were silenced by mutations in a 12.2-kb human renin promoter fused to LacZ reporter gene. This construct was used to generate transgenic mice (RENMut-LacZ). The expression of the transgene was correctly targeted to the juxtaglomerular portions of renal afferent arterioles which express endogenous mouse renin. Therefore, enhCRE and CNRE do not seem to be relevant for the control of the cell-specific expression of the human renin gene. The β-adrenoreceptor agonist isoproterenol (10 mg/kg/day, for 2 days) stimulated the endogenous renin, but not the LacZ mRNA expression. Treatment of RENMut-LacZ mice with the angiotensin converting enzyme inhibitor (enalapril 10 mg/kg/day, for 7 days) or their crossing to angiotensin receptor type 1a knockout mice led to increased renin and LacZ mRNA levels. Renin expression was upregulated by low-salt diet (0.03% NaCl, for 10 days) and downregulated by high-salt diet (4% NaCl, for 10 days). In contrast, low-salt diet did not influence, while high-salt diet inhibited the expression of LacZ. In summary, enhCRE and CNRE appear to be necessary for the transactivation of the human renin gene through β-adrenoreceptors and by low-salt diet. Our data also suggest that different intracellular mechanisms mediate the effect of low- and high-salt intake on renin expression in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
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46. Enhanced tubuloglomerular feedback in mice with vascular overexpression of A1 adenosine receptors.
- Author
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Oppermann, Mona, Yan Qin, En Yin Lai, Eisner, Christoph, Lingli Li, Yuning Huang, Mizel, Diane, Fryc, Justyna, Wilcox, Christopher S., Briggs, Josephine, Schnermann, Jurgen, and Castrop, Hayo
- Subjects
- *
ADENOSINES , *TRANSGENIC mice , *SMOOTH muscle , *ACTIN , *INTRONS , *GENE expression , *GLOMERULAR filtration rate - Abstract
Adenosine 1 receptors (A1AR) in the kidney are expressed in the vasculature and the tubular system. Pharmacological inhibition or global genetic deletion of Al AR causes marked reductions or abolishment of tubuloglomerular feedback (TGF) responses. To assess the function of vascular A1AR in TGF, we generated transgenic mouse lines in which A1AR expression in smooth muscle was augmented by placing A1AR under the control of a 5.38-kb fragment of the rat smooth muscle α-actin promoter and first intron (12). Two founder lines with highest expression in the kidney [353 ± 42 and 575 ± 43% compared with the wild type (WT)] were used in the experiments. Enhanced expression of A1AR at the expected site in these lines was confirmed by augmented constrictor responses of isolated afferent arterioles to administration of the A1AR agonist N6-cyclohexyladenosine. Maximum TGF responses (0-30 nI/min flow step) were increased from 8.4 ± 0.9 mmHg in WT (n = 21) to 14.2 ± 0.7 mmHg in A1AR-transgene (tg) 4 (n = 22; P < 0.0001), and to 12.6 ± 1.2 mmHg in A1AR-tg7 (n = 12; P < 0.02). Stepwise changes in perfusion flow caused greater numerical TGF responses in A1AR-tg than WT in all flow ranges with differences reaching levels of significance in the intermediate flow ranges of 7.5-10 and 10-15 nl/min. Proximal-distal single-nephron glomerular filtration rate (SNGFR) differences (free-flow micropuncture) were also increased in A1AR-tg, averaging 6.25 ± 1.5 nl/min compared with 2.6 ± 0.51 nI/min in WT (P = 0.034). Basal plasma renin concentrations as well as the suppression of renin secretion after volume expansion were similar in A1AR-tg and WT mice, suggesting lack of transgene expression in juxtaglomerular cells. These data indicate that A1AR expression in vascular smooth muscle cells is a critical component for TGF signaling and that changes in renal vascular Al AR expression may determine the magnitude of TGF responses. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
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47. Development of vascular renin expression in the kidney critically depends on the cyclic AMP pathway.
- Author
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Neubauer, Björn, Machura, Katharina, Min Chen, Weinstein, Lee S., Oppermann, Mona, Sequeira-Lopez, Maria Luisa, Gomez, R. Ariel, Schnermann, Jürgen, Castrop, Hayo, Kurtz, Armin, and Wagner, Charlotte
- Subjects
- *
VASCULAR resistance , *ASPARTIC proteinases , *KIDNEY diseases , *ADENYLATE cyclase , *BLOOD vessels - Abstract
During metanephric kidney development, renin expression in the renal vasculature begins in larger vessels, shifting to smaller vessels and finally remaining restricted to the terminal portions of afferent arterioles at the entrance into the glomerular capillary network. The mechanisms determining the successive expression of renin along the vasculariaxis of the kidney are not well understood. Since the cAMP signaling cascade plays a central role in the regulation of both renin secretion and synthesis in the adult kidney, it seemed feasible that this pathway might also be critical for renin expression during kidney development. In the present study we determined the spatiotemporal development of renin expression and the development of the preglomerular arterial tree in mouse kidneys with renin cell-specific deletion of Gsα, a core element for receptor activation adenylyl cyclases. We found that in the absence of the Gsα protein, renin expression was largely absent in the kidneys at any developmental stage, accompanied by alterations in the development of the preglomerular arterial tree. These data indicate that the maintenance of renin expression following a specific spatiotemporal pattern along the preglomerular vasculature critically depends on the availability of Gsα. We infer from our data that the cAMP signaling pathway is not only critical for the regulation of renin synthesis land secretion in the mature kidney but that it also is critical for establishing the juxtaglomerular expression site of renin during development. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
48. Mechanisms of tubular volume retention in immune-mediated glomerulonephritis.
- Author
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Gadau, Juliane, Peters, Harm, Kastner, Christian, Kühn, Hartmut, Nieminen-Kelhä, Melina, Khadzhynov, Dmytro, Krämer, Stephanie, Castrop, Hayo, Bachmann, Sebastian, and Theilig, Franziska
- Subjects
- *
GLOMERULONEPHRITIS , *HEMATURIA , *EDEMA , *GLOMERULAR filtration rate , *KIDNEY glomerulus , *CELL membranes - Abstract
Glomerulonephritis is characterized by hematuria, proteinuria, hypertension, and edema, but the mechanisms contributing to volume disorders are controversial. Here we used the rat anti-Thy1 model of mesangioproliferative glomerulonephritis to test the hypothesis that disturbed salt and water homeostasis is based on tubular epithelial changes that cause salt retention. In this model there was an early onset of pronounced proteinuria and lipiduria associated with reduced fractional sodium excretion and a lowering of the renin–angiotensin–aldosterone system. The glomerular filtration rate and creatinine clearance were decreased on day 6. There was a reduced abundance of the major salt and water transport proteins on the proximal tubular brush border membrane and which paralleled cellular protein overload, enhanced membrane cholesterol uptake and cytoskeletal changes. Alterations in thick ascending limb were moderate. Changes in the collecting ducts were characterized by an enhanced abundance and increased subunit cleavage of the epithelial sodium channel, both events consistent with increased sodium reabsorption. We suggest that irrespective of the proximal tubular changes, altered collecting duct sodium reabsorption may be crucial for volume retention in acute glomerulonephritis. We suggest that enhanced proteolytic cleavage of ion transporter subunits might be a novel mechanism of channel activation in glomerular diseases. Whether these proteases are filtered or locally secreted awaits determination.Kidney International (2009) 75, 699–710; doi:10.1038/ki.2008.649; published online 4 February 2009 [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
49. Altered leptin secretion in hyperinsulinemic mice under hypoxic conditions
- Author
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Östreicher, Iris, Meißner, Udo, Plank, Christian, Allabauer, Ida, Castrop, Hayo, Rascher, Wolfgang, and Dötsch, Jörg
- Subjects
- *
LEPTIN , *HYPOXEMIA , *INSULIN , *ADIPOSE tissues , *MESSENGER RNA , *GENE expression , *TRANSCRIPTION factors , *LABORATORY mice - Abstract
Abstract: Objective: Hypoxia and insulin are known key players in the activation leptin transcription and translation in vivo and in vitro. These insulin- and hypoxia-dependent effects are leptin transcription are mediated via independent elements on the leptin-promotor, even more coincubation of the two stimuli in vitro results in a supraadditive effect on leptin transcription. The aim of this study was to examine whether hyperinsulinemia is able to interfere with the hypoxia-driven expression of leptin in adipose and extra-adipose tissue in vivo. Research methods and procedures: We used the KK/HlJ mouse strain as a model for hyperinsulinemia and C57BL/6J mice as control. These two groups were exposed to hypoxia for 12 h. Serum levels of insulin and leptin were analyzed by ELISA, mRNA expression of leptin was measured via real-time PCR. Results: In the hyperinsulinemic KK/HlJ mice, hypoxia was not able to further increase the amount of leptin in serum. Instead, a significant decrease of insulin levels was detected, while serum leptin and insulin levels increased in C57BL/6J. Analysis of leptin mRNA expression in subcutaneous fat, mesenteric fat and kidney revealed that hypoxia induces leptin transcription in kidneys of C57/BL6 but not in hyperinsulinemic animals. In contrast, leptin expression in adipose tissue was not increased during hypoxia. Discussion: We conclude that leptin regulation during hypoxia in vivo depends at least in part on the modulating role of insulin. The hypoxia driven induction of insulin expression in C57/BL6 animals may be responsible for the stimulation of leptin transcription. In contrast, already hyperinsulinemic animals showed no induction — neither of insulin nor leptin after short-term hypoxia. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
50. Magnetic resonance imaging of urea transporter knockout mice shows renal pelvic abnormalities.
- Author
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Jacob, Vinitha A., Harbaugh, Calista M., Dietz, John R., Fenton, Robert A., Kim, Soo M., Castrop, Hayo, Schnermann, Jurgen, Knepper, Mark A., Chung-Lin Chou, and Anderson, Stasia A.
- Subjects
- *
MICE , *ANIMAL models in research , *MAGNETIC resonance imaging , *BLOOD pressure measurement , *HYDRONEPHROSIS , *MEDICAL research - Abstract
Many transgenic and knockout mice with increased urine flow have structural abnormalities of the renal pelvis and inner medulla. Here, we used high resolution contrast enhanced T1-weighted magnetic resonance imaging of mice whose urea transporters UT-A1 and UT-A3 were deleted (UT-A1/3−/− mice) as a model for the in vivo study of such abnormalities. Three distinct variations in the appearance of the renal pelvis were found. These included normal kidneys with no accumulation of contrast agent in the renal pelvis; infrequent frank right-sided unilateral hydronephrosis with marked atrophy of the renal medulla; and a renal pelvic reflux pattern characterized by the presence of contrast agent in the renal pelvis surrounding the renal inner medulla but no substantial atrophy of the medulla. This last pattern was found in most of the advanced age UT-A1/3−/− mice and in aquaporin-1 knockout mice. The UT-A1/3−/− mice also had increased mean arterial blood pressures. Feeding the mice a low protein diet did not prevent development of their renal pelvic abnormalities. Our studies show that real time imaging of renal pelvic structure in genetically manipulated mice provides a tool for the non-destructive, temporal evaluation of kidney structure.Kidney International (2008) 74, 1202–1208; doi:10.1038/ki.2008.392; published online 13 August 2008 [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
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