Back to Search
Start Over
Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice.
- Source :
-
Pflügers Archiv: European Journal of Physiology . Nov2003, Vol. 447 Issue 2, p214-222. 9p. - Publication Year :
- 2003
-
Abstract
- Based on the controversy about the relevance of cyclooxygenase-2 (Cox-2)-derived prostanoids from the macula densa for the control of the renin system, this study aimed to determine the interrelation between Cox-2 and renin expression in the mouse kidney. In control mice renin mRNA was readily detectable whilst renocortical Cox-2 mRNA abundance was at the detection limit of the RNase protection assay and no specific signals for Cox-2 were obtained by in situ hybridization or Western blot analysis. Experimental maneuvers such as low-salt diet, treatment with loop diuretics or angiotensin I converting enzyme inhibitors clearly increased renin mRNA abundance up to sevenfold, but under none of these conditions renocortical Cox-2 mRNA levels were significantly changed. Moreover, the strong stimulation of renin expression by angiotensin I-converting enzyme inhibition was not changed by the cyclooxygenase inhibitor ibuprofen, which in turn clearly lowered tissue prostanoid content. Our data suggest a marked divergence of renin and Cox-2 expression in the kidney cortex of C57Bl/6 mice with no clear evidence for a role of Cox-2-derived prostanoids from the macula densa in the regulation of renin expression. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RENIN
*ASPARTIC proteinases
*JUXTAGLOMERULAR apparatus
*KIDNEY cortex
*KIDNEYS
*MICE
Subjects
Details
- Language :
- English
- ISSN :
- 00316768
- Volume :
- 447
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Pflügers Archiv: European Journal of Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 16860618
- Full Text :
- https://doi.org/10.1007/s00424-003-1157-1