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International audience; Cryptosporidiosis is an infection caused by protozoan parasites belonging to the genus Cryptosporidium which is responsible for a potentially severe disease in new-born ruminants. This infection is highly prevalent in small ruminants throughout the world, especially in pre-weaned animals. The clinical expression is different between goat kids and lambs, the infection being generally more severe in the former. Molecular data demonstrate geographical variations in the species of Cryptosporidium infecting small ruminants. They also support the possibility of transmission of zoonotic species from these hosts to humans. Studies are still needed on molecular epidemiology, especially in goats, and on ways to control infection

International audience; This study was conducted to determine the prevalence and risk factors for Cryptosporidium infection in calf neonates on dairy farms in Normandy. Fecal samples were randomly collected between July 2010 and September 2011 from 968 calves (7-21 days old) on 97 farms. Up to 10 calves were selected and sampled per farm, and feces examined for oocysts by microscopy. C. parvum oocyst shedding was scored semi-quantitatively (0-5). A questionnaire about calf-level care and management was completed, and mortality rates were obtained from the French national registration database (BDNI). Bivariable and multivariable analyses of potential risk factors for C. parvum oocyst shedding were conducted using generalized estimating equation (GEE) models (family=Binomial).Overall, 402 out of 968 calves (41.5%) were positive for oocysts, and 25.1% of animals had a shedding score >2. Seven of the 97 farms (7%) were negative for oocysts in all fecal samples. At the time of collection, 375 calves (39%) had diarrhea, and its prevalence strongly correlated with the score for C. parvum oocyst shedding (p

Goats raised in pasture are inevitably infected by gastrointestinal nematodes, whatever the place and the climatic conditions. This parasitism results in production losses (growth or milk) and in some cases, in high mortality rates (with high parasite burden or in kids). For many years, these infections were controlled by conventional anthelmintics. Due to unsuitable usages (for example, high number of treatments or under-dosage), anthelmintic resistance has developed and is now very prevalent in goats as demonstrated by the numerous cases of simple or multiple anthelminthic resistance which have been reported throughout the world. Reports include resistance to the most recent anthelmintics, macrocyclic lactones and monepantel. Consequently, the way of managing gastrointestinal parasitism of goats has to move from anthelmintics alone to a more integrated management, including better use of anthelmintics, natural dewormers (nutraceuticals), enhancement of the immunity of the goats via alimentation or vaccination, selection of resistant goats or breeds and grazing management. The present chapter will give an overview of the situation regarding anthelmintic resistance in goats and integrated parasitism management.

International audience; Preliminary results obtained in calves, lambs and goat kids infected by Cryptosporidium sp. have indicated a partial prophylactic efficacy of halofuginone lactate when administered at 100μg/kg body weight (BW). In this study, the efficacy of halofuginone lactate was evaluated in goat neonates experimentally inoculated with Cryptosporidium parvum oocysts per oral route. The trial consisted in 2 replicated experiments carried out successively at 2 months of interval. Twenty-two 2- to 4-day-old kids were experimentally inoculated once, 2-3 days after the arrival in premises, with 10(6)C. parvum oocysts per oral route and were allocated into 2 groups. Animals of group 1 acted as untreated control whereas animals of group 2 received halofuginone lactate for 10 days from the infection day to day 9 post-infection (DPI) at a daily oral dose rate of 100μg/kg BW. Individual oocyst shedding was monitored by daily examination of faecal smears stained by carbol fuchsin and scored semi-quantitatively (0-5) until 19 DPI. Daily diarrhoea scores, weight gain and mortality were recorded. In the first experiment, oocyst excretion started 1 DPI in the control group, was highest on 4 DPI (mean score 3.6) and became undetectable from 16-19 DPI. In the treated group, oocyst shedding started 1 day later, showed lower scores compared to control on 4, 5, 6, 7 and 10 DPI and vanished from 16 to 19 DPI. No significant difference was seen for weight gains between groups. Five kids died in the control group compared to 1 kid in the treated group. In the second (replicated) experiment, oocyst excretion started 2 DPI in the control group, was highest on 4 DPI (mean score 4.5) and became undetectable 18 and 19 DPI. In the treated group, oocyst shedding started 2 days later, peaked on 13 DPI (mean score 2.3) and persisted until the end of the experiment. No significant difference was seen for weight gains between groups. Ten kids died in the control group compared to 3 kids in the treated group. The results demonstrated the efficacy of halofuginone lactate when given as a prophylactic treatment at 100μg/kg BW during 10 days in reducing oocyst shedding, diarrhoea and mortality in goat kid cryptosporidiosis.

Eighteen pre-weaned female calves from a single beef cattle herd in western France were sampled weekly from birth to 21/2 months of age in order to characterize Gyptosporidium oocyst output. 182 fecal samples were screened for the presence of oocysts after concentration using immunofluorescence analysis. DNA was extracted from positive samples and a PCR-RFLP protocol, with the restriction enzyme SspI and MboII, to amplify the partial SSU rRNA gene was performed. For the subtyping of Cryptosporidium parvum, a gp60 PCR was carried out. All animals excreted oocysts at at least one sampling date and 80% of the calves presented a mild diarrhea at at least one occasion, with no mortality. The prevalence of excretion reached 94% when calves were 17-23 days of age. The mean number of oocysts at the peak of excretion (10-16 days) was 5 x 10(5) oocysts per gram of feces. PCR-RFLP analysis was successful for 61 of 84 positive samples: 14 were identified as C. parvum, 15 as Cryptosporidium bovis, and 22 as Cryptosporidium ryanae. Ten mixed infections with all combinations of these species were also identified. Calves excreted the following Cryptosporidium species: C. parvum between 7 and 27 days of age, C bovis between 11 and 38 days and C ryanae from 19 to 72 days. The IIaA15G2R1 zoonotic subtype of C. parvum subtype was the only subtype identified. We observed the presence of different Cryptosporidium species depending on the age of the animals. This study showed that C parvum and C. bovis can infect beef calf neonates at similar levels of oocyst excretion with or without clinical signs and that C parvum isolates had zoonotic potential. (C) 2013 Elsevier B.V. All rights reserved.

A longitudinal study was undertaken to characterize the course of Cryptosporidium infection in a dairy goat farm located in western France. Two cohorts of twenty-five and fifteen animals, respectively, were sampled once a week from birth to weaning. Each individual fecal sample was screened using direct immunofluorescence (IFT) and if found positive, the Cryptosporidium species was identified using PCR analysis. Seventeen (68% [95% CI: 44–91]) animals were positive at least once during the first study and 14 (93% [95% CI: 80–100]) during the second, after IFT examination. In the first study, the age at first excretion was 17 days and the peak of excretion (mean arithmetic excretion: 22,700 oocysts per gram (opg) of feces) was recorded when kids were between 22 and 28 days old. For the second study, the age at first excretion was 10 days and the peak of excretion (mean arithmetic excretion: 3.4 × 10 6 opg) was recorded in animals aged between 10 and 14 days. Clinical signs were observed only in animals of the second cohort. DNA sequence analysis at the 18S ribosomal RNA locus was successful for 9 of the 27 IFT-positive samples in the first cohort and for 10 of the 34 positive isolates in the second cohort. All isolates were identified as Cryptosporidium xiaoi except one which was identified as Cryptosporidium parvum. Our results confirm that goat kids are hosts for C. parvum and C. xiaoi and that infection by C. xiaoi may be associated with mild clinical signs.

International audience; Resistance to ivermectin and moxidectin was explored by a faecal egg count reduction test in two sheep flocks with suspected anthelmintic resistance. The FECRT confirmed one suspicion, with a mean percentage of reduction in egg excretion within the treated groups of 0% for ivermectin (CI 95%: -228 to 58) and 13% for moxidectin (CI 95%: -152 to 70). This was further explored by a controlled efficacy test. An experimental infection of 18 naive lambs was set up using infective larvae isolated from this flock (5000 L3/lamb). Compared to the control group, abomasal worm burdens (Teladorsagia circumcincta) were reduced by 90% [CI 95%: 81.5-94.8] and 85% [CI 95%: 72.4-92.2] after ivermectin (p < 0.05) and moxidectin (p < 0.05) treatment respectively. Again, compared to the control group, there was a reduction for intestinal strongyles (Trichostrongylus colubriformis) of 100% and 99% [CI 95%: 97.5-99.7] for ivermectin and moxidectin respectively. No difference was found between the efficacy of moxidectin and ivermectin. Pharmacokinetic values indicated that the strongyles were submitted to anthelmintic concentrations usually lethal to them. This trial demonstrated the first multiple resistance of ovine strongyles in France.

Within Europe, the goat industry appears to have minor importance when compared to sheep or cattle productions, and the interest of research and studies in caprine pathology is limited. Here, this article is aimed at presenting the main research models in France, the current available data on the pathogenic mechanisms and the physiopathological processes and the related development of measures of management control using examples of pathologies caused by different agents (prions, viruses, bacteria, protozoan or helminthic parasites) that affect goats and their production. It is also aimed at underlining the interest of specific caprine studies not only as models of comparative pathology in small ruminants but also as tools that take into account the specific constraints of the dairy industry.; La chèvre, souvent désignée comme «la vache du pauvre», est aussi le parent pauvre des études en pathologie chez les petits ruminants. Les références caprines ne constituent que 25 à 30% des travaux cités pour les petits ruminants dans leur ensemble. De nombreux exemples soulignent pourtant les dangers potentiels de négliger les spécificités physiologiques, métaboliques ou comportementales des caprins dans la maîtrise des processus pathologiques. Ce constat justifie que les caprins fassent l’objet de recherches particulières dont les résultats en pathologie comparée conduisent à formuler des hypothèses de portée générale

International audience; Coccidiosis (Eimeriosis sensu stricto) of small ruminants is a protozoan infection caused by several species of the genus Eimeria which develop in the small and the large intestine, affect young animals in particular and are specific for each host. Eimeria ovinoidalis in sheep and Eimeria ninakohlyakimovae in goats are the most pathogenic species. Coccidiosis is of great economic importance because of the losses due to clinical disease (diarrhoea) but also because of subclinical infections (poor weight gain in particular). Oocyst excretion is at the maximum around the weaning period and shows a steady decline afterwards due to a strong immunity. Risk factors for high excretion include breeding intensification, high stocking rates in premises, poor hygiene and all causes of stress (physiological, nutritional, etc.). Reliable diagnosis include combined clinical, epidemiological, necropsic and coproscopical approaches. Control is mainly based on hygienic measures between lambing/kidding and weaning periods and on anticoccidial compounds use

Amongst the alternative strategies to the use of anthelmintics, the administration of Duddingtonia flagrans spores has already proved its efficacy in reducing the number of developing larvae of several nematode species in goat faeces. In this trial, the efficacy of this fungus against the larvae of the three major nematode species of goats was compared in various conditions of coproculture. Twelve strongyle free goats were experimentally infected with either Trichostrongylus colubriformis, Teladorsagia circumcincta or Haemonchus contortus larvae. Half of the animals received an oral dose of 5x10(5) Duddingtonia chlamydospores/kg BW daily for 27 days, whereas the remaining was kept as control goats. From the 7th day of administration onwards, individual coproscopical examinations as well as coprocultures, which were incubated 4, 7, 10 or 14 days at 21 or 28 degrees C, were performed. The reduction in developing larvae due to the activity of Duddingtonia ranged from 62.8 to 99.5% compared to control. The trapping efficacy depended on temperature (better activity of the fungus at 21 than at 28 degrees C) and on duration (larval reductions lower after 4 days than after 7, 10 and 14 days of coproculture). Teladorsagia larvae were the least trapped, and Haemonchus larvae were the most trapped.

Due to the high level of anthelmintic resistance in goat nematode populations in France, there is an obvious need to develop alternative strategies to control parasitic infections in grazing animals. The purpose of this work was first to assess the efficacy of the nematode trapping fungus Duddingtonia flagrans on the development of infective larvae of Trichostrongylus colubriformis in feces. Eighteen culled strongyle free dairy goats were experimentally infected with 7500 infective larvae of a T. colubriformis benzimidazole resistant strain. Half of the animals received an oral dose of 5×105 D. flagrans chlamydospores/kg body weight (BW) during 9 days whereas the remaining was kept as controls. From the 6th day of administration onwards, coproscopical examinations as well as standard coprocultures incubated 13 days at 26 °C were performed on all the animals. The reduction in developing T. colubriformis larvae due to activity by D. flagrans ranged from 86 to 96% compared to control. The second objective was to determine an eventual interaction in the feces between the growth and the trapping activity of D. flagrans and the presence of benzimidazole (BZD) compounds, some of which having a high antifungal activity. In a first experiment, the growth of D. flagrans spores was measured on Sabouraud medium with increasing concentrations of thiabendazole (TBZ, 1–200 μg/ml) or albendazole (ABZ, 1–10 μg/ml) added. In a second experiment, the activity of D. flagrans against BZD resistant T. colubriformis larvae was assessed in feces with TBZ solution added (final concentration of 100 μg TBZ/g of feces) compared to controls without TBZ or without D. flagrans spores. Finally, the larval development of BZD resistant T. colubriformis in feces was compared from goats fed or not fed D. flagrans chlamydospores (5×105/kg BW) and either treated or not with TBZ (50 mg/kg BW) or ABZ (5 mg/kg BW). The results clearly indicated a very high fungicidal activity of TBZ against D. flagrans on Sabouraud medium from 10 μg/ml whereas no antagonistic activity could be measured for ABZ because of solvent toxicity. When adding directly TBZ upon the feces or when collecting feces 24 h or 36 h after drenching the animals (TBZ or ABZ), the effect of these drugs on the activity of D. flagrans could not have been assessed because of the absence of larval development due to the BZD compounds. These results showed that the level of BZD compounds in feces immediately after drenching was probably so high that a nearly total inhibition of larval development occurred even when a resistant strain is concerned. Ninety-six hours after drenching, the full predatory activity of D. flagrans was displayed again, indicating that no long term inhibition due to BZD residues could be suspected.

The high prevalence of benzimidazole-resistant nematodes in French grazing dairy goat flocks led to a search for nematode-control schemes based on integrated approaches with non-chemical options, like vaccination, grazing management, or biological control using nematophagous fungi. The effect of the daily feeding of goats with spores of the nematophagous fungus Duddingtonia flagrans on third-stage larvae (L3) of Teladorsagia circumcincta was examined in faecal cultures. In addition, the effect of D. flagrans on the survival of first-stage larvae (L1) of Muellerius capillaris was tested. Twenty-two culled dairy goats previously raised in a zero-grazing system were twice infected at monthly intervals with 5,000 and then 7,500 T. circumcincta L3. Eight animals were infected with a benzimidazole-susceptible (BZs) strain while the remainder received a benzimidazole-resistant one (BZr). Six culled goats naturally infected with M. capillaris were purchased from private farms. All the goats were divided in two groups, one group receiving daily 5 x 10(5) chlamydospores of D. flagrans/kg body weight per goat for seven consecutive days in the food, the other group acting as control. For T. circumcincta-infected goats, individual egg counts and coprocultures (13 days, 25 degrees C) followed by L3 extraction with the Baermann method were performed. For M. capillaris-infected goats, extraction of L1 with the Baermann apparatus was individually performed on day 0 and after coprocultures on days 7, 10 and 14. Reductions in percentage development of T. circumcincta L3 in fungus groups compared with control groups ranged from 84% (BZs strain) to 90% (BZr strain). A decrease in M. capillaris L1 recovery was noted on days 7 and 10 (a reduction of 70% compared with day 0) and on day 14 (85%), but this pattern was similar in both groups, whether receiving the fungus or not. At the dosage of 5 x 10(5) spores/kg body weight, D. flagrans was highly effective in reducing the larval development of T. circumcincta in goats faeces. In contrast, the fungus did not reduce M. capillaris L1 survival in faeces in our conditions.

International audience; Cohorts of pre-weaned calves were studied for Cryptosporidium infection over three successive years (2010-2012) in one beef cattle herd in western France. Each year 25-34 calves were sampled weekly from 3 days to one month of age in order to characterize oocyst output, Cryptosporidium species and clinical features associated with infection. Faecal samples were screened for the presence of oocysts using immunofluorescence analysis. DNA was extracted from positive samples and a PCR SSU rRNA followed by RFLP or sequencing was performed. For the subtyping of C. parvum, a gp60 PCR was carried out. Regardless of the year, 92-100% of the animals excreted oocysts on at least one sampling date. Depending on the year of observation, the age of highest prevalence varied. In contrast, the peak of excretion was systematically observed almost at the same age (2nd-3rd week of life) with excretion levels ranging from between 100 and 1.7×10(7)oocysts/g of faeces. Differences concerning clinical signs depending on the year of sampling were observed. Different species patterns were observed, with a predominance of C. bovis in the 1st year and a predominance of C. parvum in the last year. Moreover, two zoonotic subtypes of C. parvum, IIaA15G2R1 and IIaA18G2R1, were recorded in different years. This study shows that, in a given farm, the Cryptosporidium species and C. parvum subtypes identified as well as the prevalence of infection and level of excretion may vary greatly and show distinct patterns according to the year.

Cryptosporidium spp. is an important agent of neonatal diarrhoea in goat kids. Little is known about its molecular characterization in adult goats. A longitudinal study was set up to identify the species excreted by adult goats around parturition. Individual faecal samples were collected from 20 pregnant adult goats between 1 and 5 years old in one flock. Samplings began 3 weeks before the estimated kidding date and were done weekly until kidding and for 2 weeks after kidding. Cryptosporidium oocysts were concentrated from 15 g of faeces using a caesium chloride (CsCl) method. Oocyst output was determined using a direct immunofluorescent antibody test (IFAT). Genomic DNA was extracted from each CsCl-concentrated faecal sample positive by IFAT and submitted to a nested PCR-RFLP on the SSU rDNA gene followed by sequencing to identify the isolates at species level. According to their kidding date, goats were sampled between 4 and 8 times. Sixteen goats, out of the eighteen which kidded, were found positive at least at one sampling date. Infection was asymptomatic. Prevalence of excretion was maximal 14 days before kidding with half of the goats excreting oocysts at this date. Excretion was higher before kidding than after kidding. Unexpected levels of excretion were observed with individual oocyst excretion ranging from 6 to 2.5 × 10(5) oocysts per gram of faeces. All isolates were identified as Cryptosporidium ubiquitum.

Infection of grazing goats by Calicophoron daubneyi has already been described in France, in particular in the Saone-et-Loire area (eastern France). The aim of the present study was to describe more precisely the distribution of this parasite in this area. Forty-two goat farms using pasture were visited once at the end of 2006: 15 goats per farm were individually sampled for faeces and the farmer filled in a questionnaire on general characteristics of the farm, on the use of pasture and on the management of parasitism. C. daubneyi infection was equally present in the whole area. 58.1% of the farms presented at least one animal excreting C. daubneyi eggs. Intra-flock prevalence was on average 35% and varied from 7% to 93%. 72.5% of the positive goats excreted less than 100 eggs/g. 70% of the farmers treating their goats for trematode infections used oxyclozanide with two main doses, 15 and 22 mg/kg bodyweight (BW).

International audience; The objective of the present study was to evaluate the characteristics of two rapid tests, namely, a faecal smear staining method (Heine staining) and a commercially available immunochromatographic (IC) assay, against a direct immunofluorescent antibody test (IFAT) for the diagnosis of Cryptosporidium infections in 917 faecal samples from calves aged 1,000,000opg. Additional scoring from 1 to 3 according to the thickness of the sample line for the IC test correlated with increasing levels of Cryptosporidium opg measured by IFAT in the range of 10,000 to >1,000,000opg. In conclusion, both Heine staining and the IC test can be reliably used through a simple semi-quantitative scale for grossly quantifying oocyst output in calf faecal samples.

Cryptosporidium is a protozoan parasite responsible for gastroenteritis, especially in immunocompromised patients. Laboratory diagnosis of cryptosporidiosis relies on microscopy, antigen detection, and nucleic acid detection and analysis. Among the numerous molecular targets available, the 18S rRNA gene displays the best sensitivity and sequence variations between species and can be used for molecular typing assays. This paper presents a new real-time PCR assay for the detection and quantification of all Cryptosporidium species associated with the identification of Cryptosporidium hominis and Cryptosporidium parvum . The sensitivity and specificity of this new PCR assay were assessed on a multicentric basis, using well-characterized Cryptosporidium -positive and -negative human stool samples, and the efficiencies of nine extraction methods were comparatively assessed using Cryptosporidium -seeded stool samples and phosphate-buffered saline samples. A comparison of extraction yields showed that the most efficient extraction method was the Boom technique in association with mechanical grinding, and column extraction showed higher binding capacity than extraction methods based on magnetic silica. Our PCR assay was able to quantify at least 300 oocysts per gram of stool. Satisfactory reproducibility between laboratories was observed. The two main species causing human disease, Cryptosporidium hominis and Cryptosporidium parvum , were identified using a duplex real-time PCR assay with specific TaqMan minor-groove-binding ligand (MGB) probes for the same amplicon. To conclude, this one-step quantitative PCR is well suited to the routine diagnosis of cryptosporidiosis since practical conditions, including DNA extraction, quantification using well-defined standards, and identification of the two main species infecting humans, have been positively assessed.

International audience; Ninety-two Cryptosporidium sp.-positive fecal samples of dairy diarrheic or non-diarrheic calves from 30 cattle herds in Normandy (France) were selected. Here, the aim was to investigate the species of Cryptosporidium excreted as well as the subtypes of Cryptosporidium parvum found in 7-17-day-old dairy calves. Excretion levels were comprised between 2 × 10(4) and 4 × 10(7) oocysts per gram of feces. Here, a nested 18S SSU rRNA PCR associated with sequencing was performed for identification of Cryptosporidium species and revealed the presence of C. parvum in most cases (80/82), except for two animals which were infected with Cryptosporidium bovis. Then, C. parvum samples were submitted to gp60 PCR. For 39 samples from 24 different herds, a multilocus analysis based on four mini-microsatellites loci (MM19, MM5, MSF, and MS9-Mallon) were conducted. These results were combined with sequence analysis of the gp60 to obtain multilocus types (MLTs). Here, C. parvum gp60 genotyping identified three subtypes in the IIa zoonotic allele family: IIaA15G2R1 (88%), IIaA16G3R1 (10%), and IIaA19G2R1 (2%), and we identified 12 MLTs. The MS9-Mallon locus was reported as the most polymorphic (five alleles). The most common MLT was MLT 1 with 15 samples in 10 farms: (MS9-M: 298, MSF: 165, MM5: 264, MM19: 462, and gp60 subtype: IIaA15G2R1). When comparing diarrheic and non-diarrheic fecal samples, no difference was seen for distribution of Cryptosporidium species, C. parvum gp60 subtypes, and MLTs. Here, in a range of oocyst excretion of 10(4)-10(7) opg, both in diarrheic and non-diarrheic calves, infection was mainly due to C. parvum and to the zoonotic subtype: IIaA15G2R1.

International audience; Studies on excretion and molecular characterization of Cryptosporidium have been mostly conducted in dairy calves, both diarrhoeic and non-diarrhoeic. Little is known about Cryptosporidium in beef calves, especially in non-diarrhoeic ones. This study was conducted in a herd of Parthenais beef cattle (France) with no history of clinical cryptosporidiosis. Twenty-five calves were sampled once a week from birth to one month of age (age range: 5-34 days). At each sampling date, presence of clinical signs of cryptosporidiosis (diarrhoea) was recorded. Oocyst excretion was assessed using the Heine staining method and a direct immunofluorescence method (Merifluor(®) C/G) which allowed quantification (oocysts per gram of faeces, opg). All samples were subjected to a two-step nested PCR protocol to amplify the 18S rRNA gene and amplification products were sequenced. None of the calves presented diarrhoea. Twenty-three of them excreted oocysts at least one sampling date. Prevalence of excretion was maximal when calves were 27-34 days old, with a percentage of excretion of 85% in this age category [95% CI: 70; 100]. Mean excretion was maximal when calves were 20 to 26 days old, with a mean excretion of 7.6×10(5) opg (range: 0-8×10(6) opg). 32 isolates were successfully identified: 27 as Cryptosporidium bovis, 4 as Cryptosporidium ryanae and 1 as Cryptosporidium parvum. C. bovis was isolated from samples of calves between 11 and 33 days old. C. ryanae was isolated from samples of calves between 17 and 34 days old. C. parvum was isolated from one calf aged 13 days. This survey demonstrated the high infection rate of non-diarrhoeic beef calves by Cryptosporidium species other than C. parvum.

The environmental impact of Duddingtonia flagrans, a potential biological control agent for nematode parasites, was tested in a 2-year-plot study using goat faeces. The trial assessed the impact of fungal presence on the disintegration of faeces and on non-target, free-living soil nematode populations. Three groups of goats experimentally infected by Trichostrongylus colubriformis received three different doses of D. flagrans chlamydospores (0 chlamydospores/kg body weight (BW), 0.5 × 106 chlamydospores/kg BW or 5 × 106 chlamydospores/kg BW). One hundred grams of faeces containing T. colubriformis eggs and D. flagrans chlamydospores at three different concentrations were deposited on pasture plots on four different occasions: May 2003, September 2003, June 2004 and September 2004. Faeces were weighed 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 weeks after deposit and immediately afterwards replaced to their initial positions. In addition, soil samples were taken just below faecal deposits to evaluate the impact of fungal presence on non-target free-living nematodes. Results showed that there was no treatment effect on the pellet degradation rate. Analysis of soil nematode fauna failed to demonstrate any effect of the dose rate of 0.5 × 106 chlamydospores/kg BW, while a reduction of the number of free-living nematodes was seen for the maximal chlamydospore concentration at autumn sets.

Many compounds have been screened for their potential anti-cryptosporidial activity in ruminants but none of them has been totally efficient in controlling the disease. A product containing activated charcoal and wood vinegar liquid demonstrated a good efficacy in controlling clinical signs and oocyst excretion in calves experimentally infected. This product (Obionekk®, Obione, Charentay, France) was given to goat kids in field conditions. The product was administered in a preventive way at the dose rate of 3.75 g/day/kid of Obionekk® from the age of 3 days to the age of 17 days in suspension in milk replacer twice or three times a day. A significant reduction of oocyst excretion was observed when the product was given 3 times a day when compared to control group. The clinical signs (diarrhoea) were significantly reduced whatever the rhythm of distribution when compared to control group. These results demonstrated the efficacy of Obionekk® in preventing cryptosporidiosis in goat kids in field conditions.

Anthelmintic resistance is very prevalent in ruminant strongyle populations, especially in goats. Several occurrences of multiple anthelminthic resistances have been reported in goat flocks throughout the world, including resistance to the most recent macrocyclic lactones. A faecal egg count reduction test was conducted to detect resistance to ivermectin in French goat flocks. Thirty goats per flock were randomly selected in 22 flocks and allocated into two groups of 15 animals: an untreated control group and an ivermectin-treated group (0.3 mg/kg BW per os). Individual faecal egg counts and pooled larval cultures were performed 16-17 days after anthelmintic treatment for control and treated groups. FECR’s were calculated for treated group vs. control one and when

Many compounds have been screened for their potential anti-cryptosporidial activity in ruminants but none of them has been totally efficient in controlling the disease. Macrolide antibiotics have demonstrated some activity against Cryptosporidium spp. in humans. Tilmicosin is a macrolide antibiotic, available in France in an oral form (Pulmotil® AC, Lilly France). The preventive efficacy of tilmicosin was evaluated in a goat farm experiencing severe clinical cryptosporidiosis in kids. Twenty-two kids were separated from their dams just after birth and placed in a separated pen. They were divided into 3 groups: an untreated group (10 kids), group 1 (6 kids) receiving tilmicosin at 25 mg/kg BW/day and group 2 (6 kids) receiving tilmicosin at 50 mg/kg BW/day. Tilmicosin was individually given by oral route from day 2 of age for 10 days. Three times a week, individual faecal samples were performed to assess the oocyst output. Clinical data, i.e. diarrhea and mortality, were recorded. In control kids, the highest prevalence and intensity of excretion were observed between day 6 and day 16 of age and mortality reached 90%. Excretion dynamic and clinical consequences were similar in both treated kid groups. Finally, tilmicosin did not demonstrate any activity against severe kid cryptosporidiosis in field conditions

ANTHELMINTIC resistance in nematodes of small ruminants has been reported on all continents ([Kaplan 2004][1]). In France, several cases of benzimidazole resistance have been reported in goats ([Chartier and Pors 1994][2], [Cabaret and others 1995][3], [Chartier and others 1998][4], 2001).

Paramphistome infections are very common in ruminants and may induce clinical signs, but little is known about effective treatments. In this study, the efficacy of oxyclozanide against Calicophoron daubneyi was studied in goats and its activity tested against immature stages (10 days post-infection) at a dose of 22.5mg/kg bodyweight (BW) and against adult stages using two doses (15 and 22.5mg/kg BW). There was a reduction (82%) in the number of immature worms (compared to controls) but the result was not statistically significant. When tested against adult stages, however, oxyclozanide reduced the worm burdens by 95.6% and 95.9% at doses of 15 and 22.5mg/kg BW, respectively, with no significant difference between the two doses. The experiment demonstrated that oxyclozanide is highly effective in reducing the number of adult paramphistomes in goats.

A field trial, conducted over two consecutive years, was aimed at assessing the efficacy of the administration of spores of the nematophagous fungus Duddingtonia flagrans to young goats for the control of nematode parasite infections on a French commercial dairy goat flock. For both years, the first-year grazing kids were divided into two similarly managed groups (fungus and control groups): in 2003 a daily dose rate of 5 x 10(5) spores/kg body weight was given to the fungus-group animals, while in 2004 a daily dose rate of 10(6) spores/kg body weight was used; the other half of the kids, acting as control, did not receive the spores. Parameters measured every 3 weeks included nematode egg excretion, larval development in faecal cultures and pasture larval counts. Additionally, at the beginning, the middle and the end of each grazing season, the goats were weighed and blood samples for pepsinogen determination were collected. In 2003, similar results were recorded for all the measured parameters in the control and fungus groups. In contrast, in 2004, the kids receiving the spores showed lower faecal egg counts and pepsinogen levels at the end of the season and higher growth rate compared to kids of the control group.

The ability of the nematophagous fungus Duddingtonia flagrans to reduce the number of infective nematode larvae in coproculture was investigated in goats using different doses of chlamydospores (0, 1.25 × 10 5 , 2.5 × 10 5 , 5 × 10 5 chlamydospores/kg BW/day) given by oral administration or by voluntary consumption in feed during natural or experimental infections with nematodes. The kinetics of excretion of D. flagrans chlamydospores in the faeces was also determined using a dose of 5 × 10 5 chlamydospores/kg BW/day for five days. For all the trials, the faecal nematode egg outputs were determined by a modified McMaster method and standard coprocultures were set up (14 days, 25 °C) to determine the number of larvae emerging from culture in fungus treated and control faeces. When chlamydospores were orally administered, the number of larvae were reduced by 50 to 97% when compared to control cultures. No difference in the level of larval emergence from the culture was seen for experimental or natural infections at the different chlamydospore dose rates. In contrast, when chlamydospores were distributed in the feed, a dose- dependent relationship was observed 10 days after the start of administration, the larval development being 2.0%, 14.0% and 86.9% for 5 × 10 5 , 2.5 × 10 5 and 0 spores/kg BW/day, respectively. In addition, the kinetic study showed that the larval emergence from coproculture in the fungus group was statistically lower than in the control group from the second day of administration of the chlamydospores and remained lower until the second day after the last administration (p < 0.05). The results indicate that, for goats in farm conditions, a minimum daily dose of 5 × 10 5 chlamydospores/kg BW must be used to ensure a high treatment efficacy and that daily administration is preferable for maintenance of efficacy over time. goat / biological control / Duddingtonia flagrans / nematode parasite / dose trials