47 results on '"Bednarczyk-Cwynar B"'
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2. Proapoptotic activity and ABCC1-related multidrug resistance reduction ability of semisynthetic oleanolic acid derivatives DIOXOL and HIMOXOL in human acute promyelocytic leukemia cells
- Author
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Paszel-Jaworska, A., primary, Rubiś, B., additional, Bednarczyk-Cwynar, B., additional, Zaprutko, L., additional, and Rybczyńska, M., additional
- Published
- 2015
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3. 3-Cyano-11-oxo-3,4-seco-12a-aza-C-homoolean-4(23)-en-28-oic acid methyl ester
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Froelich, A., primary, Bednarczyk-Cwynar, B., additional, and Gzella, A. K., additional
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- 2012
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4. 321 Oleanolic acid derivative methyl 3-hydroxyimino-11-oxoolean-12-en- 28-oate inhibits ABCC1/MRP1 protein function and reduces its level in acute promyelocytic leukemia cells
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Paszel, A., primary, Bednarczyk-Cwynar, B., additional, Zaprutko, L., additional, Hofmann, J., additional, and Rybczynska, M., additional
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- 2010
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5. 726 POSTER Antitumor activity and reversal of multidrug resistance by the newly synthesised oleanolic acid derivative – methyl-3, 11-dioxoolean-12-en-28-oate
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Paszel, A., Hofmann, J., Zaprutko, L., Bednarczyk-Cwynar, B., Bock, G., and Rybczynska, M.
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- 2007
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6. Hybrids of oleanolic acid with norbornene-2,3-dicarboximide-n-carboxylic acids as potential anticancer agents
- Author
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Bednarczyk-Cwynar, B., Ruszkowski, P., Atamanyuk, D., Roman Lesyk, and Zaprutko, L.
7. Exploring the Potential of Oleanolic Acid Dimers-Cytostatic and Antioxidant Activities, Molecular Docking, and ADMETox Profile.
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Günther A, Zalewski P, Sip S, and Bednarczyk-Cwynar B
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- Humans, Cell Line, Tumor, Dimerization, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Molecular Structure, Molecular Docking Simulation, Antioxidants chemistry, Antioxidants pharmacology, Oleanolic Acid chemistry, Oleanolic Acid pharmacology, Oleanolic Acid analogs & derivatives
- Abstract
The presented work aimed to explore the potential of oleanolic acid dimers (OADs): their cytostatic and antioxidant activities, molecular docking, pharmacokinetics, and ADMETox profile. The cytostatic properties of oleanolic acid ( 1 ) and its 14 synthesised dimers ( 2a - 2n ) were evaluated against 10 tumour types and expressed as IC
50 values. Molecular docking was performed with the CB-Dock2 server. Antioxidant properties were evaluated with the CUPRAC method. ADMETox properties were evaluated with the ADMETlab Manual (2.0) database. The results indicate that the obtained OADs can be effective cytostatic agents, for which the IC50 not exceeded 10.00 for many tested cancer cell lines. All OADs were much more active against all cell lines than the mother compound ( 1 ). All dimers can inhibit the interaction between the 1MP8 protein and cellular proteins with the best results for compounds 2f and 2g with unsaturated bonds within the linker. An additional advantage of the tested OADs was a high level of antioxidant activity, with Trolox equivalent for OADs 2c , 2d , 2g - 2j , 2l , and 2m of approximately 0.04 mg/mL, and beneficial pharmacokinetics and ADMETox properties. The differences in the DPPH and CUPRAC assay results obtained for OADs may indicate that these compounds may be effective antioxidants against different radicals.- Published
- 2024
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8. Oleanolic Acid Dimers with Potential Application in Medicine-Design, Synthesis, Physico-Chemical Characteristics, Cytotoxic and Antioxidant Activity.
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Günther A, Zalewski P, Sip S, Ruszkowski P, and Bednarczyk-Cwynar B
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- Humans, Cell Line, Tumor, Drug Design, Quantitative Structure-Activity Relationship, Dimerization, Cell Survival drug effects, Oleanolic Acid chemistry, Oleanolic Acid pharmacology, Oleanolic Acid analogs & derivatives, Antioxidants pharmacology, Antioxidants chemistry, Antioxidants chemical synthesis, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents chemical synthesis
- Abstract
The present work aimed to obtain a set of oleanolic acid derivatives with a high level of cytotoxic and antioxidant activities and a low level of toxicity by applying an economical method. Oleanolic acid was alkylated with α,ω-dihalogenoalkane/α,ω-dihalogenoalkene to obtain 14 derivatives of dimer structure. All of the newly obtained compounds were subjected to QSAR computational analysis to evaluate the probability of the occurrence of different types of pharmacological activities depending on the structure of the analysed compound. All dimers were tested for cytotoxicity activity and antioxidant potential. The cytotoxicity was tested on the SKBR-3, SKOV-3, PC-3, and U-87 cancer cell lines with the application of the MTT assay. The HDF cell line was applied to evaluate the tested compounds' Selectivity Index. The antioxidant test was performed with a DPPH assay. Almost all triterpene dimers showed a high level of cytotoxic activity towards selected cancer cell lines, with an IC
50 value below 10 µM. The synthesised derivatives of oleanolic acid exhibited varying degrees of antioxidant activity, surpassing that of the natural compound in several instances. Employing the DPPH assay, compounds 2a , 2b , and 2f emerged as promising candidates, demonstrating significantly higher Trolox equivalents and highlighting their potential for pharmaceutical and nutraceutical applications. Joining two oleanolic acid residues through their C-17 carboxyl group using α,ω-dihalogenoalkanes/α,ω-dihalogenoalkenes resulted in the synthesis of highly potent cytotoxic agents with favourable SIs and high levels of antioxidant activity.- Published
- 2024
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9. Acylation of Oleanolic Acid Oximes Effectively Improves Cytotoxic Activity in In Vitro Studies.
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Bednarczyk-Cwynar B and Ruszkowski P
- Abstract
(1) Background: The aim of the presented work was to obtain a set of oleanolic acid derivatives with a high level of anticancer activity and a low level of toxicity by applying an economic method. Three types of oleanolic acid derivatives were obtained: (i) derivatives of methyl oleanonate oxime, (ii) derivatives of methyl oleanonate oxime with an additional 11-oxo function, and (iii) derivatives of morpholide of oleanonic acid oxime. (2) Methods: The above oximes were acylated with aliphatic or aromatic carboxylic acid. The newly obtained compounds were subjected to ADMETox analysis and were also tested for cytotoxicity activity on the HeLa, KB, MCF-7, A-549, and HDF cell lines with the MTT assay. (3) Results: Among the tested acylated oximes of oleanolic acid, some derivatives, particularly those with two nitro groups attached to the aromatic ring, proved to be the most potent cytotoxic agents. These triterpene derivatives significantly inhibited the growth of the HeLa, KB, MCF-7, and A-549 cancer cell lines in micromolar concentrations. (4) Conclusions: The introduction of different moieties, particularly the 3,5-dinitro group, resulted in the synthesis of highly potent cytotoxic agents with favorable SI and ADMETox parameters.
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- 2024
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10. Unlocking the Potential: Novel NSAIDs Hybrids Unleash Chemopreventive Power toward Liver Cancer Cells through Nrf2, NF-κB, and MAPK Signaling Pathways.
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Narożna M, Krajka-Kuźniak V, Bednarczyk-Cwynar B, and Baer-Dubowska W
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- Humans, NF-kappa B metabolism, NF-E2-Related Factor 2 metabolism, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Signal Transduction, Cell Line, Carcinoma, Hepatocellular drug therapy, Liver Neoplasms drug therapy
- Abstract
HCC is a highly aggressive malignancy with limited treatment options. In this study, novel conjugates of non-steroidal anti-inflammatory drugs (NSAIDs)-Ibuprofen and Ketoprofen-with oleanolic acid oximes derivatives (OAO) were synthesized, and their activity as modulators of signaling pathways involved in HCC pathogenesis was evaluated in normal THLE-2 liver cells, and HCC-derived HepG2 cells. The results demonstrated that conjugation with OAO derivatives reduces the cytotoxicity of parent compounds in both cell lines. In THLE-2 cells, treatment with conjugates resulted in increased activation of the Nrf2-ARE pathway. An opposite effect was observed in HepG2 cells. In the later reduction of NF-κB, it was observed along with modulation of MAPK signaling pathways (AKT, ERK, p38, p70S6K, and JNK). Moreover, STAT3, STAT5, and CREB transcription factors on protein levels were significantly reduced as a result of treatment with IBU- and KET-OAO derivatives conjugates. The most active were conjugates with OAO-morpholide. Overall, the findings of this study demonstrate that IBU-OAO and KET-OAO derivative conjugates modulate the key signaling pathways involved in hepatic cancer development. Their effect on specific signaling pathways varied depending on the structure of the conjugate. Since the conjugation of IBU and KET with OAO derivatives reduced their cytotoxicity, the conjugates may be considered good candidates for the prevention of liver cancer.
- Published
- 2023
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11. The Effect of Oleanolic Acid and Its Four New Semisynthetic Derivatives on Human MeWo and A375 Melanoma Cell Lines.
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Bednarczyk-Cwynar B, Leśków A, Szczuka I, Zaprutko L, and Diakowska D
- Abstract
This study aimed to synthesize four new semisynthetic derivatives of natural oleanolic acid (OA) and, based on an analysis of their cytotoxic and anti-proliferative effects against human MeWo and A375 melanoma cell lines, select those with anti-cancer potential. We also screened the treatment time with the concentration of all four derivatives. We synthesized oxime 2 and performed its acylation with carboxylic acids into new derivatives 3a, 3b, 3c and 3d according to the methods previously described. Colorimetric MTT and SRB assays were used to measure the anti-proliferative and cytotoxic activity of OA and its derivatives 3a , 3b , 3c and 3d against melanoma cells. Selected concentrations of OA, the derivatives, and different time periods of incubation were used in the study. The data were analyzed statistically. The present results revealed the possible anti-proliferative and cytotoxic potential of two selected OA derivatives 3a and 3b , on A375 and MeWo melanoma cells, especially at concentrations of 50 μM and 100 μM at 48 h of incubation ( p < 0.05). Further studies will be necessary to analyze the proapoptotic and anti-cancer activities of 3a and 3b against skin and other cancer cells. The bromoacetoxyimine derivative ( 3b ) of OA morpholide turned out to be the most effective against the tested cancer cells.
- Published
- 2023
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12. Polymorphism of Butyl Ester of Oleanolic Acid-The Dominance of Dispersive Interactions over Electrostatic.
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Langer D, Wicher B, Dutkiewicz Z, Bendzinska-Berus W, Bednarczyk-Cwynar B, and Tykarska E
- Subjects
- Esters chemistry, Static Electricity, Oleanolic Acid, Glycyrrhetinic Acid
- Abstract
Oleanolic (OA) and glycyrrhetinic acids (GE), as well as their derivatives, show a variety of pharmacological properties. Their crystal structures provide valuable information related to the assembly modes of these biologically active compounds. In the known-to-date crystals of OA esters, their 11-oxo derivatives, and GE ester crystals, triterpenes associate, forming different types of ribbons and layers whose construction is based mainly on van der Waals forces and weak C-H···O interactions. New crystal structures of 11-oxo OA methyl ester and the polymorph of OA butyl ester reveal an alternative aggregation mode. Supramolecular architectures consist of helical chains which are stabilized by hydrogen bonds of O-H···O type. It was found that two polymorphic forms of butyl OA ester (layered and helical) are related monotropically. In a structure of metastable form, O-H···O hydrogen bonds occur, while the thermodynamically preferred phase is governed mainly by van der Waals interactions. The intermolecular interaction energies calculated using CrystalExplorer, PIXEL, and Psi4 programs showed that even in motifs formed through O-H···O hydrogen bonds, the dispersive forces have a significant impact.
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- 2023
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13. Biological Activity of Oleanolic Acid Derivatives HIMOXOL and Br-HIMOLID in Breast Cancer Cells Is Mediated by ER and EGFR.
- Author
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Lisiak N, Dzikowska P, Wisniewska U, Kaczmarek M, Bednarczyk-Cwynar B, Zaprutko L, and Rubis B
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- Humans, Female, Receptors, Estrogen, ErbB Receptors metabolism, Apoptosis, Cell Proliferation, Cell Line, Tumor, Oleanolic Acid pharmacology, Breast Neoplasms metabolism
- Abstract
Breast cancer is one of the most frequently observed malignancies worldwide and represents a heterogeneous group of cancers. For this reason, it is crucial to properly diagnose every single case so a specific and efficient therapy can be adjusted. One of the most critical diagnostic parameters evaluated in cancer tissue is the status of the estrogen receptor (ER) and epidermal growth factor receptor (EGFR). Interestingly, the expression of the indicated receptors may be used in a personalized therapy approach. Importantly, the promising role of phytochemicals in the modulation of pathways controlled by ER and EGFR was also demonstrated in several types of cancer. One such biologically active compound is oleanolic acid, but due to poor water solubility and cell membrane permeability that limits its use, alternative derivative compounds were developed. These are HIMOXOL and Br-HIMOLID, which were demonstrated to be capable of inducing apoptosis and autophagy or diminishing the migratory and invasive potential of breast cancer cells in vitro. In our study, we revealed that proliferation, cell cycle, apoptosis, autophagy, and also the migratory potential of HIMOXOL and Br-HIMOLID in breast cancer cells are mediated by ER (MCF7) and EGFR (MDA-MB-231) receptors. These observations make the studied compounds interesting in the context of anticancer strategies.
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- 2023
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14. Highly Branched Betulin Based Polyanhydrides for Self-Assembled Micellar Nanoparticles Formulation.
- Author
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Niewolik D, Bednarczyk-Cwynar B, Ruszkowski P, Dzido G, and Jaszcz K
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- Drug Carriers chemistry, Micelles, Polyethylene Glycols chemistry, Triterpenes, Water, Cytostatic Agents, Nanoparticles chemistry, Polyanhydrides, Prodrugs
- Abstract
Polyanhydrides based on betulin are promising materials for use in controlled drug delivery systems. Due to the broad biological activity of betulin derivatives and lack of toxicity in vitro and in vivo, these polymers can be used both as polymeric prodrug and as carriers of other biologically active compounds. In this study, we develop a novel amphiphilic branched polyanhydrides synthesized by the two-step melt polycondensation of betulin disuccinate (DBB) and a tricarboxylic derivative of poly(ethylene glycol) (PEG_COOH). DBB and PEG_COOH were used as the hydrophobic and hydrophilic segments, respectively. The content of DBB in copolymers was from 10 to 95 wt%. Copolymers were assessed for their cytostatic activity against various cancer cell lines. Compared to linear DBB and PEG-based polyanhydrides, the branched polyanhydrides exhibited higher anticancer activity. The obtained polymers were able to self-assemble in water to form micelles with hydrodynamic diameters from 144.8 to 561.8 nm. and are stable over a concentration range from 12.5 µg/mL to 6.8 mg/mL. The formed micelles were found to be spherical in shape using a scanning electron microscope. It was found that the structure and composition of polyanhydrides affected the hydrodynamic diameter of the micelles. The branched betulin-based polyanhydrides have the potential to serve as biodegradable polymer prodrugs or carriers for other bioactive compounds., Competing Interests: The authors declare no conflict of interest.
- Published
- 2022
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15. Insights into isostructural and non-isostructural crystals of esters of oleanolic acid and its 11-oxo derivatives.
- Author
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Langer D, Wicher B, Bendzinska-Berus W, Bednarczyk-Cwynar B, and Tykarska E
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- Esters chemistry, Glycyrrhetinic Acid, Oleanolic Acid chemistry
- Abstract
Synthesis and structural characterization of new esters of oleanolic acid and its 11-oxo derivatives are reported. Compounds crystallize in four isostructural groups, each containing one to four structures. Single-crystal X-ray analysis revealed that molecules belonging to non-isostructural groups self-associate according to two schemes that describe also supramolecular architectures in crystals of glycyrrhetinic acid derivatives. Structural motifs arise as a result of van der Waals forces. Parameters introduced for the analysis of one- and two-dimensional assemblies allow the comparison of motifs in isostructural and non-isostructural crystals, including polymorphs, and a qualitative assessment of differences in molecular self-assembly. One-, two- or three-dimensional similarity has been confirmed by XPac calculations.
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- 2022
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16. Biodegradable and Bioactive Carriers Based on Poly(betulin disuccinate- co -sebacic Acid) for Rifampicin Delivery.
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Niewolik D, Bednarczyk-Cwynar B, Ruszkowski P, Kazek-Kęsik A, Dzido G, and Jaszcz K
- Abstract
This paper describes the preparation and characterization of polymer-drug systems based on polymeric microspheres obtained from poly(betulin disuccinate- co -sebacic acid). The active compound that was coupled to the betulin-based carriers was rifampicin (RIF), an ansamycin drug used in the treatment of tuberculosis. Poly(betulin disuccinate- co -sebacic acid) microspheres were prepared using a solvent evaporation technique from copolymers obtained by polycondensation of betulin disuccinate ( DBB ) and sebacic acid ( SEB ). The content of sebacic acid in the copolymers was 20, 40, 60 and 80 wt%, respectively. Small and large rifampicin-loaded microspheres were obtained for each of the copolymers. The initial amount of drug was 10, 30 or 50 wt%, based on the weight of the polymer. Particles obtained in this study were round in shape with diameter in the range of 2-21 μm and of orange to red colour originating from rifampicin. The RIF encapsulation efficacy varied from 7% to 33%. Drug loading varied from 2% to 13% and increased at a higher RIF ratio. The highest degree of drug loading was observed for large particles, in which the initial amount of drug (at the particle preparation stage) was 50 wt%. Microspheres prepared from betulin-based polyanhydrides may have significant applications in drug delivery systems. The concentration of loaded drug was enough to obtain bactericidal effects against reference S. Aureus ATCC 25923 bacteria.
- Published
- 2022
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17. Multivariate assessment of anticancer oleanane triterpenoids lipophilicity.
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Pastewska M, Bednarczyk-Cwynar B, Kovačević S, Buławska N, Ulenberg S, Georgiev P, Kapica H, Kawczak P, Bączek T, Sawicki W, and Ciura K
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- Chromatography, High Pressure Liquid, Chromatography, Reverse-Phase, Oleanolic Acid analogs & derivatives, Triterpenes
- Abstract
Naturally occurring molecules are excellent sources of lead compounds. A series of oleanolic acid (OA) derivatives previously synthesized in our laboratory, which show promising antitumor activity, have been analyzed in terms of lipophilicity evaluation applying chromatographic and computational approaches. Retention data obtained on three reversed-phase liquid chromatography stationary phases (RP-HPLC) and immobilized artificial membrane chromatography (IAM-HPLC) were compared with computational methods using chemometric tools such as cluster analysis, principal component analysis and sum of ranking differences. To investigate the molecular mechanism of retention quantitive structure retention relationship analysis was performed, based on the genetic algorithm coupled with multiple linear regression (GA-MLR). The obtained results suggested that the ionization potential of studied molecules significantly affects their retention in classical RP-HPLC. In IAM-HPLC additionally, polarizability-related descriptors also play an essential role in that process. The lipophilicity indices comparison shows significant differences between the computational lipophilicity and chromatographically determined ones., Competing Interests: Declaration of Competing Interest None., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2021
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18. Oleanolic Acid's Semisynthetic Derivatives HIMOXOL and Br-HIMOLID Show Proautophagic Potential and Inhibit Migration of HER2-Positive Breast Cancer Cells In Vitro.
- Author
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Lisiak NM, Lewicka I, Kaczmarek M, Kujawski J, Bednarczyk-Cwynar B, Zaprutko L, and Rubis B
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- Apoptosis, Breast Neoplasms genetics, Breast Neoplasms metabolism, Cell Cycle, Cell Movement, Cell Proliferation, Female, Humans, In Vitro Techniques, Oleanolic Acid pharmacology, Tumor Cells, Cultured, Autophagy, Breast Neoplasms pathology, Oleanolic Acid analogs & derivatives, Oleanolic Acid chemistry, Receptor, ErbB-2 metabolism
- Abstract
Approximately 20-30% of the diagnosed breast cancers overexpress the human epidermal growth factor receptor 2 (HER2). This type of cancer is associated with a more aggressive phenotype; thus, there is a need for the discovery of new compounds that would improve the survival in HER2-positive breast cancer patients. It seems that one of the most promising therapeutic cancer strategies could be based on the biological activity of pentacyclic triterpenes' derivatives and the best-known representative of this group, oleanolic acid (OA). The biological activity of oleanolic acid and its two semisynthetic derivatives, methyl 3-hydroxyimino-11-oxoolean-12-en-28-oate (HIMOXOL) and 12α-bromo-3-hydroxyimonoolean-28→13-olide (Br-HIMOLID), was assessed in SK-BR-3 breast cancer cells (HER2-positive). Viability tests, cell cycle assessment, evaluation of apoptosis, autophagy, and adhesion/migration processes were performed using MTT, clonogenic, cytofluorometry, Western blot, and qPCR. Both derivatives revealed higher cytotoxicity in studied breast cancer cells than the maternal compound, OA. They also decreased cell viability, induced autophagy, and (when applied in sub-cytotoxic concentrations) decreased the migration of SK-BR-3 cells.This study is the first to report the cytostatic, proautophagic (mTOR/LC3/SQSTM/BECN1 pathway), and anti-migratory (integrin β1/FAK/paxillin pathway) activities of HIMOXOL and Br-HIMOLID in HER2-positive breast cancer cells.
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- 2021
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19. Conjugation of Diclofenac with Novel Oleanolic Acid Derivatives Modulate Nrf2 and NF-κB Activity in Hepatic Cancer Cells and Normal Hepatocytes Leading to Enhancement of Its Therapeutic and Chemopreventive Potential.
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Narożna M, Krajka-Kuźniak V, Bednarczyk-Cwynar B, Kucińska M, Kleszcz R, Kujawski J, Piotrowska-Kempisty H, Plewiński A, Murias M, and Baer-Dubowska W
- Abstract
Combining NSAIDs with conventional therapeutics was recently explored as a new strategy in cancer therapy. Our earlier studies showed that novel oleanolic acid oximes (OAO) conjugated with aspirin or indomethacin may enhance their anti-cancer potential through modulation of the Nrf2 and NF-κB signaling pathways. This study focused on the synthesis and biological evaluation of four diclofenac (DCL)-OAO derivative conjugates in the context of these pathways' modification and hepatic cells survival. Treatment with the conjugates 4d , 3-diclofenacoxyiminoolean-12-en-28-oic acid morpholide, and 4c , 3-diclofenacoxyiminoolean-12-en-28-oic acid benzyl ester significantly reduced cell viability in comparison to the DCL alone. In THLE-2, immortalized normal hepatocytes treated with these conjugates resulted in the activation of Nrf2 and increased expression in SOD-1 and NQO1, while the opposite effect was observed in the HepG2 hepatoma cells. In both cell lines, reduced activation of the NF-κB and COX-2 expression was observed. In HepG2 cells, conjugates increased ROS production resulting from a reduced antioxidant defense, induced apoptosis, and inhibited cell proliferation. In addition, the OAO morpholide derivative and its DCL hybrid reduced the tumor volume in mice bearing xenografts. In conclusion, our study demonstrated that conjugating diclofenac with the OAO morpholide and a benzyl ester might enhance its anti-cancer activity in HCC.
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- 2021
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20. Bioactive Betulin and PEG Based Polyanhydrides for Use in Drug Delivery Systems.
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Niewolik D, Bednarczyk-Cwynar B, Ruszkowski P, Sosnowski TR, and Jaszcz K
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- A549 Cells, Aerosols, Cell Line, Tumor, Drug Carriers chemistry, HeLa Cells, Hep G2 Cells, Humans, Hydrogen-Ion Concentration, Hydrolysis, MCF-7 Cells, Magnetic Resonance Spectroscopy, Microspheres, Molecular Weight, Particle Size, Polymers chemistry, Porosity, Solvents, Drug Delivery Systems, Phytochemicals chemistry, Polyanhydrides chemistry, Polyethylene Glycols chemistry, Triterpenes chemistry
- Abstract
In the course of this study, a series of novel, biodegradable polyanhydrides based on betulin disuccinate and dicarboxylic derivatives of poly(ethylene glycol) were prepared by two-step polycondensation. These copolymers can be used as carriers in drug delivery systems, in the form of microspheres. Betulin and its derivatives exhibit a broad spectrum of biological activity, including cytotoxic activity, which makes them promising substances for use as therapeutic agents. Microspheres that were prepared from betulin based polyanhydrides show promising properties for use in application in drug delivery systems, including inhalation systems. The obtained copolymers release the active substance-betulin disuccinate-as a result of hydrolysis under physiological conditions. The use of a poly(ethylene glycol) derivative as a co-monomer increases the solubility and bioavailability of the obtained compounds. Microspheres with diameters in the range of 0.5-25 µm were prepared by emulsion solvent evaporation method and their physicochemical and aerodynamic properties were analyzed. The morphological characteristics of the microspheres depended on the presence of poly(ethylene glycol) (PEG) segment within the structure of polyanhydrides. The porosity of the particles depended on the amount and molecular weight of the PEG used and also on the speed of homogenization. The most porous particles were obtained from polyanhydrides containing 20% wt. of PEG 600 by using a homogenization speed of 18,000 rpm.
- Published
- 2021
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21. The Effect of Novel Oleanolic Acid Oximes Conjugated with Indomethacin on the Nrf2-ARE And NF-κB Signaling Pathways in Normal Hepatocytes and Human Hepatocellular Cancer Cells.
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Narożna M, Krajka-Kuźniak V, Bednarczyk-Cwynar B, Kleszcz R, Kujawski J, and Baer-Dubowska W
- Abstract
Nrf2 and NF-κB play a key role in inflammation-driven cancers. Conjugation of anti-inflammatory drugs with oleanolic acid oxime (OAO) may enhance their therapeutic potential as a result of downregulation of these pathways. Novel OAO derivatives conjugated with indomethacin (IND) were synthesized, and their effect on the activation and expression of Nrf2 and NF-κB in HepG2 hepatoma cells and THLE-2 immortalized normal hepatocytes was evaluated in relation to cell cycle arrest and apoptosis. Treatment with OAO-IND conjugates reduced the activation of Nrf2 and NF-κB and the expression of their active forms in HepG2 cells, while in normal hepatocytes, the activation of Nrf2 was increased and NF-κB diminished. Compounds 3d , 3-indomethacinoxyiminoolean-12-en-28-oic acid morpholide, and 3c , 3-indomethacinoxyiminoolean-12-en-28-oic acid benzyl ester, were the most efficient. In THLE-2 cells, as opposed to HepG2 cells, the expressions of SOD-1 and NQO1 were significantly enhanced after treatment with these compounds. The COX-2 expression was diminished in both cell lines. OAO-IND derivatives affected the cell cycle arrest at G2/M, leading to increased apoptosis and increased number of resting HepG2 cells. Therefore, the conjugation of IND with OAO derivatives may preserve cancer cells against chemoresistance through the inhibition of the Nrf2-ARE pathway and NF-κB and, at the same time, exert a chemopreventive effect in normal hepatocytes.
- Published
- 2020
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22. Activation of the Nrf2 response by oleanolic acid oxime morpholide (3-hydroxyiminoolean-12-en-28-oic acid morpholide) is associated with its ability to induce apoptosis and inhibit proliferation in HepG2 hepatoma cells.
- Author
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Narożna M, Krajka-Kuźniak V, Kleszcz R, Bednarczyk-Cwynar B, Szaefer H, and Baer-Dubowska W
- Subjects
- Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular metabolism, Carcinoma, Hepatocellular pathology, Extracellular Signal-Regulated MAP Kinases metabolism, G2 Phase Cell Cycle Checkpoints drug effects, Gene Expression Regulation, Neoplastic, Hep G2 Cells, Humans, Kelch-Like ECH-Associated Protein 1 genetics, Kelch-Like ECH-Associated Protein 1 metabolism, Liver Neoplasms genetics, Liver Neoplasms metabolism, Liver Neoplasms pathology, Phosphorylation, Reactive Oxygen Species metabolism, Signal Transduction, Antineoplastic Agents pharmacology, Apoptosis drug effects, Carcinoma, Hepatocellular drug therapy, Cell Proliferation drug effects, Liver Neoplasms drug therapy, NF-E2-Related Factor 2 metabolism
- Abstract
Our previous study demonstrated that new oleanolic acid oxime (OAO) derivatives and their conjugates with aspirin (ASP) inhibit NF-κB activation. Evidence exists that the downregulation of NF-κB negatively interferes with the Nrf2 signaling pathway. This study aimed to evaluate the effect of these compounds on Nrf2 activation and its cellular consequences in human hepatoma HepG2 cells and immortalized normal hepatocytes THLE-2. The results showed the enhanced activation and expression of Nrf2 as a result of treatment with OAO derivatives themselves and to less extent by their ASP conjugates, mainly in HepG2 cells. The association between cytotoxicity evaluated in our previous study and Nrf2 activation was observed. In this regard, compounds (18) with morpholide substituent at the C-17 position of OAO molecule and (12) with methyl ester substituent at the same position of OAO molecule to the most extent activated Nrf2 and subsequently cell cycle arrest at G2/M, leading to increased apoptosis and the number of resting HepG2 cells. The derivative of OAO (18) substituted with ASP (19) also affected Nrf2 activation and expression, but this effect was less pronounced in comparison with non-conjugated OAO. However, conjugation enhanced Nrf2 activation in normal THLE-2 cells. These results confirmed our earlier suggestion that OAO derivatives conjugated with ASP have the potential for application in the liver cancer chemoprevention. OAO themselves, particularly OAO substituted with morpholide, may be considered therapeutic agents, which may support conventional treatment strategy. Further studies are required to confirm this suggestion., (Copyright © 2020. Published by Elsevier B.V.)
- Published
- 2020
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23. Oleanolic acid oxime derivatives and their conjugates with aspirin modulate the NF-κB-mediated transcription in HepG2 hepatoma cells.
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Krajka-Kuźniak V, Bednarczyk-Cwynar B, Paluszczak J, Szaefer H, Narożna M, Zaprutko L, and Baer-Dubowska W
- Subjects
- Hep G2 Cells, Humans, Oleanolic Acid chemistry, Signal Transduction drug effects, Aspirin chemistry, NF-kappa B metabolism, Oleanolic Acid pharmacology, Oximes chemistry, Transcription, Genetic drug effects
- Abstract
The aim of this study was to evaluate the effect of new oleanolic acid oxime (OAO) derivatives and their conjugates with aspirin (ASP) on the expression and activation of NF-κB in human hepatoma HepG2 cells. OAO derivatives showed a stronger cytotoxic effect against HepG2 cells compared with their conjugates with aspirin. Moreover, conjugation of OAO with ASP led to enhanced downregulation of NF-κB expression and activation. Among the hybrids with ASP, compounds: 19, 3-(2-acetoxy)benzoyloxyiminoolean-12-en-28-oic acid morpholide and 13, 3-(2-acetoxy)benzoyloxyiminoolean-12-en-28-oic acid methyl ester, differing, respectively, in morpholide and methyl ester groups at the C-17 position of oleanolic acid (OA) molecule were the most efficient. COX-2 transcript and protein levels were also diminished after treatment with these compounds. The results of this study indicate that the new derivatives of OAO and particularly their conjugates with ASP, downregulate the expression of COX-2 in HepG2 cells by modulating the NF-κB signaling pathway and suggest their potential application in the prevention of liver inflammation and cancer., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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24. Morpholide derivative of the novel oleanolic oxime and succinic acid conjugate diminish the expression and activity of NF-κB and STATs in human hepatocellular carcinoma cells.
- Author
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Krajka-Kuźniak V, Bednarczyk-Cwynar B, Narożna M, Szaefer H, and Baer-Dubowska W
- Subjects
- Carcinoma, Hepatocellular, Cell Survival drug effects, Cyclooxygenase 2 genetics, Cyclooxygenase 2 metabolism, Hep G2 Cells, Humans, Liver Neoplasms, NF-kappa B genetics, Oximes chemistry, Proto-Oncogene Proteins c-myc genetics, Proto-Oncogene Proteins c-myc metabolism, STAT Transcription Factors genetics, Transcription, Genetic drug effects, bcl-2-Associated X Protein genetics, bcl-2-Associated X Protein metabolism, bcl-X Protein genetics, bcl-X Protein metabolism, NF-kappa B metabolism, Oleanolic Acid analogs & derivatives, Oximes pharmacology, STAT Transcription Factors metabolism, Succinic Acid chemistry
- Abstract
Naturally occurring oleanolic acid (OA) possesses a hepatoprotective activity and ability to inhibit proliferation of human hepatocellular carcinoma cells. Both properties might be related to its anti-inflammatory activity. Its low bioavailability justifies the search for more hydrophilic OA derivatives. The aim of this study was the design and synthesis of four novel OA oxime derivatives conjugated with succinic acid at the C-3 position of oleanane skeleton structure and evaluation of their effect on NF-κB and STATs expression and activation in HepG2 cells. The expression of NF-κB and cyclooxygenase-2 (COX-2), STAT5A/B and STAT3 with its target genes: BAX, BCL-XL and MYC was evaluated after 24 h treatment with tested compounds. The comparison of the levels of cytosolic and nuclear NF-κB subunits p50, p65 and STATs proteins was used as the measure of their activation. The results pointed out the 3-succinyloxyiminoolean-12-en-28-oic acid morpholide (SMAM) as the most potent modulator of NF-κB and STAT3. SMAM significantly reduced the expression and activation of NF-κB as well as its nuclear protein level of p65 subunit. This compound also reduced the expression and activation of STAT3 and STAT5A/B. Combined effect of SMAM on these transcription factors resulted in reduced expression of COX-2, MYC and anti-apoptotic BCL-XL genes. Simultaneously, the increased expression of pro-apoptotic BAX gene was observed. In the cells treated with 3-succinyloxyiminoolean-12-en-28-oic acid (SMAA) the increased expression of BAX was also found. The effects of 3-succinyloxyiminoolean-12-en-28-oic acid benzyl ester (SMAEB) and 3-succinyloxyiminoolean-12-en-28-oic acid methyl ester (SMAEM) were moderate and ambiguous in relation to the tested factors. Moreover, the coordinated action of SMAM on NF-κB and STAT3 confirms their close association in HepG2 cells. We conclude that SMAM efficiently downregulates the key elements of signaling pathways involved in inflammatory driven HCC. Thus, may be considered as a potential chemopreventive or therapeutic agent in this type of cancer., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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25. Novel polymeric derivatives of betulin with anticancer activity.
- Author
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Niewolik D, Krukiewicz K, Bednarczyk-Cwynar B, Ruszkowski P, and Jaszcz K
- Abstract
In order to provide novel polymeric biomaterials for chemotherapeutic purposes, in this paper we described the synthesis and the characterization of the physicochemical properties of a betulin-based polyanhydride exhibiting anti-cancer effects. The polyanhydride was obtained by a melt polycondensation of a disuccinate betulin (3,28-di- O -succinyl betulin), and was thoroughly characterized through
1 H NMR and13 C NMR spectroscopies, correlation spectroscopy, heteronuclear single quantum correlation, size exclusion chromatography, differential scanning calorimetry and FT-IR spectroscopy. It was confirmed, that the obtained polyanhydride undergoes hydrolytic degradation, releasing disuccinate betulin as a degradation product. Polyanhydride of a disuccinate betulin was tested for cytostatic activity against a wide range of cancer cell lines (HeLa, MCF-7, A-549, U-87MG, KB and HepG2), proving its efficiency in inhibiting the growth of selected cancer cells. To realize the concept of an easily administrated drug release system, polyanhydride was fabricated in a form of micro- (1-30 μm) and nanospheres (∼400 nm) by using an emulsion solvent evaporation method. The micro- and nanospheres were characterized by SEM., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)- Published
- 2019
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26. Enhancing anticancer activity through the combination of bioreducing agents and triterpenes.
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Bednarczyk-Cwynar B, Ruszkowski P, Jarosz T, and Krukiewicz K
- Subjects
- Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Cell Line, Tumor, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Humans, Molecular Conformation, Oleanolic Acid chemistry, Structure-Activity Relationship, Triterpenes chemistry, Antineoplastic Agents pharmacology, Oleanolic Acid pharmacology, Triterpenes pharmacology
- Abstract
Aim: Triterpenes are natural compounds, whose wide biological activity predestines them for application as promising new chemotherapeutics. In this paper, we report the results of our investigations into the substitution of oleanolic acid with aromatic and nitroaromatic moieties acting as bioreducing agents., Results: The process of reduction of nitro groups was investigated through cyclic voltammetry, UV-Vis and electron paramagnetic resonance spectroelectrochemistry. The cytotoxic activity against selected cancer cell lines was determined, showing a significant increase in cytotoxicity when the triterpene is equipped with a nitroaromatic moiety., Conclusion: We believe this approach to the functionalization is promising in terms of enhancing anticancer activity. We also indicate electrochemical techniques as advantageous preclinical screening methods for the identification of cytotoxic agents.
- Published
- 2018
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27. Synthesis and antiproliferative properties of new hydrophilic esters of triterpenic acids.
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Eignerova B, Tichy M, Krasulova J, Kvasnica M, Rarova L, Christova R, Urban M, Bednarczyk-Cwynar B, Hajduch M, and Sarek J
- Subjects
- Cell Line, Tumor, Drug Screening Assays, Antitumor, Esters chemistry, Humans, Spectrum Analysis methods, Triterpenes chemistry, Cell Proliferation drug effects, Triterpenes chemical synthesis, Triterpenes pharmacology
- Abstract
To improve the properties of cytotoxic triterpenoid acids 1-5, a large set of hydrophilic esters was synthesized. We choose betulinic acid (1), dihydrobetulinic acid (2), 21-oxoacid 3 along with highly active des-E lupane acids 4 and 5 as a model set of compounds for esterification of which the properties needed to be improved. As ester moieties were used - methoxyethanol and 2-(2-methoxyethoxy)ethanol and glycolic unit (type a-d), pyrrolidinoethanol, piperidinoethanol and morpholinoethanol (type f-h), and monosaccharide groups (type i-l). As a result, 56 triterpenic esters (49 new compounds) were obtained and their cytotoxicity on four cancer cell lines and normal human fibroblasts was tested. All new compounds were fully soluble at all tested concentrations, which used to be a problem of the parent compounds 1 and 2. 16 compounds had IC
50 < 10 μM on at least one cancer cell line, 12 compounds had cytotoxicity of <10 μM against at least three of four tested cancer cell lines. The highest activity was found for compound 3c (1.8 μM on MCF7, 2.8 μM on HeLa, and 1.6 μM on G-361 cells) which also had no toxicity on non-cancerous BJ fibroblasts at the highest tested concentration (50 μM). High selective cytotoxicity was also found in compounds 1k, 2k, 3c, and 3i that are ideal candidates for drug development. Therefore, more studies to identify the mechanism of action were performed in case of 1k, 3c, and 3g such as effects on cell cycle and apoptosis. It was found that compounds 3c and 3g can induce apoptosis via caspase-3 activation and modulation of protein Bcl-2 in G-361 cells. In conclusion, compounds 1k, 3c, and 3g show high and selective cytotoxicity, therefore they are significantly better candidates for anti-cancer drug development than the parent acids 1-5., (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)- Published
- 2017
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28. HYBRIDS OF OLEANOLIC ACID WITH NORBORNENE-2,3-DICARBOXIMIDE-N- CARBOXYLIC ACIDS AS POTENTIAL ANTICANCER AGENTS.
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Bednarczyk-Cwynar B, Ruszkowskp P, Atamanyuk D, Lesyk R, and Zaprutko L
- Subjects
- Cell Survival drug effects, Dose-Response Relationship, Drug, Drug Discovery methods, HeLa Cells, Hep G2 Cells, Humans, Inhibitory Concentration 50, Molecular Structure, Oleanolic Acid analogs & derivatives, Structure-Activity Relationship, Technology, Pharmaceutical methods, Antineoplastic Agents chemical synthesis, Antineoplastic Agents pharmacology, Carboxylic Acids chemical synthesis, Carboxylic Acids pharmacology, Norbornanes chemical synthesis, Norbornanes pharmacology, Oleanolic Acid chemical synthesis, Oleanolic Acid pharmacology
- Abstract
The synthesis and cytotoxic activity of new oleanolic acid derivatives (8a-c and 9a-c) are presented. The obtained compounds are hybrids of oleanolic acid oximes and carboxylic acids containing short alkyl chains linked with nitrogen atom of norbomene-2,3-dicarboximide moieties via the nitrogen atom. The structures of the obtained new compounds (8a-c and 9a-c) were confinmed by spectral data. The derivatives 8a-c and 9a-c were subjected to the MTT assay in order to evaluate their cytotoxic activity towards HeLa, KB, MCF-7, HepG2 and HDF cell lines in comparison to mother compound (oleanolic acid, 1). Among the tested oximes acylated with carboxylic acids containing norbomene-imide moieties, the derivative 8b, with a propionoxyimino linker, exhibited the most advantageous level of cytotoxicity, with IC50 values from 2.75 pM (for MCF-7 cells) to 4.36 pM (for HDF cells).
- Published
- 2017
29. Semisynthetic oleanane triterpenoids inhibit migration and invasion of human breast cancer cells through downregulated expression of the ITGB1/PTK2/PXN pathway.
- Author
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Lisiak N, Paszel-Jaworska A, Totoń E, Rubiś B, Pakuła M, Bednarczyk-Cwynar B, Zaprutko L, and Rybczyńska M
- Subjects
- Cell Line, Tumor drug effects, Cell Movement drug effects, Down-Regulation, Focal Adhesion Kinase 1 genetics, Humans, Integrin beta1 genetics, Neoplasm Invasiveness, Oleanolic Acid pharmacology, Paxillin genetics, Paxillin metabolism, Signal Transduction, Antineoplastic Agents pharmacology, Breast Neoplasms drug therapy, Focal Adhesion Kinase 1 metabolism, Integrin beta1 metabolism, Oleanolic Acid analogs & derivatives
- Abstract
This paper reports a study on the role of two synthetic derivatives of oleanolic acid (OA), HIMOXOL and Br-HIMOLID, in the regulation of cell migration and invasion and the underlying molecular mechanisms of breast cancer cells. The effect of the compounds on four breast cancer cell lines (MCF7, MDA-MB-231, MDA-MB-468, and T-47D) and also on noncancerous breast cells, MCF-12A, was reported. The compounds had no effect on the migration of MCF-12A cells. However, both the derivatives revealed a higher cytotoxicity than the maternal compound OA, and in sub-cytotoxic concentrations, they decreased the migration of MCF7, MDA-MB-231, and MDA-MB-468 breast cancer cells and also the invasion of MCF7 and MDA-MB-231 cells; although, the derivatives had no effect on the migration and invasion of T-47D cells. Both the derivatives of OA inhibited the cell migratory and invasive abilities of breast cancer cells by downregulating the expressions of ITGB1, PTK2, and PXN genes and by decreasing the phosphorylation status and the level of its respective proteins (integrin β1, FAK, and paxillin, respectively). This study is the first to report the antimigratory and anti-invasive activities of HIMOXOL and Br-HIMOLID in breast cancer cells., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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30. Betulin-loaded PEDOT films for regional chemotherapy.
- Author
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Krukiewicz K, Cichy M, Ruszkowski P, Turczyn R, Jarosz T, Zak JK, Lapkowski M, and Bednarczyk-Cwynar B
- Subjects
- Cell Death drug effects, Electrochemistry, Humans, KB Cells, MCF-7 Cells, Polymerization, Spectrometry, X-Ray Emission, Spectrophotometry, Infrared, Antineoplastic Agents pharmacology, Bridged Bicyclo Compounds, Heterocyclic chemistry, Polymers chemistry, Triterpenes pharmacology
- Abstract
Chemotherapy is one of the most commonly used cancer treatments. Even so, it has significant adverse effects on healthy tissues. These effects can be avoided through the use of regional chemotherapy, an approach based on delivering the anti-cancer agents locally, to the site of cancer tissue accumulation. Among the different classes of biomaterials that are used as drug carriers, conducting polymers allow reversible, electrostatic immobilization and controlled release of a variety of compounds. In this work, we describe a method for producing surfaces possessing anti-cancer activity, which are a potential tool for regional chemotherapy. Our method consists of covering the surface with a conducting polymer matrix, followed by loading that matrix with cytotoxic compounds. We have chosen betulin as the model compound for this study, as it is commonly available triterpene that exhibits cytotoxicity against a variety of tumor cell lines. The presence of betulin in the polymer matrix is confirmed by SEM, EDS and IR spectroscopy. The release of betulin is carried out using two protocols, i.e. passive mode (open circuit conditions) or active (application of constant potential) mode. The biological activity of betulin that was released from the matrix is confirmed by its toxic effect against KB and MCF-7 cancer cell lines (IC
50 values of 13.34±0.88μg/mL and 12.57±1.81μg/mL for KB and MCF-7, respectively). The described method of surface modification is shown to be an effective mean of producing surfaces that possess anti-cancer activity, serving as advantageous materials for regional chemotherapy applications., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2017
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31. Advances in Chemistry and Pharmacology of Triterpenoid Synthetic Dimers.
- Author
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Bednarczyk-Cwynar B and Günther A
- Subjects
- Animals, Anti-Inflammatory Agents chemical synthesis, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Antiviral Agents chemical synthesis, Antiviral Agents chemistry, Antiviral Agents pharmacology, Cyclization, Diabetes Mellitus drug therapy, Humans, Hypoglycemic Agents chemical synthesis, Hypoglycemic Agents chemistry, Hypoglycemic Agents pharmacology, Neoplasms drug therapy, Triterpenes chemical synthesis, Virus Diseases drug therapy, Viruses drug effects, Chemistry Techniques, Synthetic methods, Dimerization, Triterpenes chemistry, Triterpenes pharmacology
- Abstract
This review focuses on advances in chemistry and pharmacology of synthetic triterpenoid dimers, obtained from natural compounds. Synthetic triterpenoid dimers are divided into specific subgroups based on the structure of main triterpenoid monomeric skeleton. Synthetic triterpenoid derivatives of dimeric structure can be obtained through the covalent linkage of the C-3 hydroxyl or another group, via the C-2 atom or the C-17 carboxyl group (mainly anhydrides, amides or esters). Some triterpenes can undergo chemical transformations leading to the formation of cyclic dimers or other types of dimers. Most of the obtained triterpenoid dimers have been subjected to pharmacological tests evaluating their biological activity, mainly antiviral (HIV-1 RT, HCVpp, VSVpp, HIV-RT-C8166-CCR5), cytotoxic (against e.g. 388, MCF-7, SF-268, NCIH460, KM20L2, DU-145, Hep-G2, A549, BGC-823, PC-3), anti-inflammatory (iNOS, RAW 264.7) and antidiabetic (RMGPa inhibition). The authors also reported the ability of some of the obtained cyclic triterpenoid dimers to recognize anions and to form self-assembled structures., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Published
- 2017
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32. Cytotoxic Activity of Some Lupeol Derivatives.
- Author
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Bednarczyk-Cwynar B, Wiecaszek T, and Ruszkowski P
- Subjects
- A549 Cells, HeLa Cells, Humans, KB Cells, MCF-7 Cells, Molecular Structure, Antineoplastic Agents pharmacology, Pentacyclic Triterpenes pharmacology, Triterpenes pharmacology
- Abstract
The synthesis of 3-0-acyl derivatives of lupeol is presented, as well as lupenone and the oxime of lupenone. The obtained derivatives were subjected to the MTT test in order to evaluate their cytotoxic activity toward HeLa, KB, MCF-7 and A-549 cell lines in comparison with lupeol (1). The IC₅₀ values for lupeol (1) and all of the tested derivatives (2-6) were from about 7-10 μM for lupenone (5) to about 81 μM for o-phthaloyl-lupeol (4). Acylation of the C-3 hydroxy function of compound 1 decreased the cytotoxic activity of compounds 2-4. The most active anticancer agent was lupenone (5), with IC₅₀ values 7.1-9.1 μM in comparison with 37.7-51.9 μM for lupeol (1).
- Published
- 2016
33. Strong and Long-Lasting Antinociceptive and Anti-inflammatory Conjugate of Naturally Occurring Oleanolic Acid and Aspirin.
- Author
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Bednarczyk-Cwynar B, Wachowiak N, Szulc M, Kamińska E, Bogacz A, Bartkowiak-Wieczorek J, Zaprutko L, and Mikolajczak PL
- Abstract
The conjugate 8 was obtained as a result of condensation of 3-hydroxyiminooleanolic acid morfolide (7) and aspirin in dioxane. Analgesic effect of OAO-ASA (8) for the range of doses 0.3-300.0 mg/kg (p.o.) was performed in mice using a hot-plate test. Anti-inflammatory activity was assessed on carrageenan-induced paw edema in rats for the same range of doses. The conjugate OAO-ASA (8) did not significantly change locomotor activity of mice, therefore sedative properties of the compound should be excluded. The compound 8 proved a simple, proportional, dose-dependent analgesic action and expressed strong anti-inflammatory activity showing a reversed U-shaped, dose-dependent relation with its maximum at 30.0 mg/kg. After its combined administration with morphine (MF, 5.0 mg/kg, s.c.) the lowering of antinociceptive activity was found; however, the interaction with naloxone (NL, 3.0 mg/kg, s.c.) did not affect the antinociceptive effect of OAO-ASA (8), therefore its opioid mechanism of action should be rather excluded. After combined administration with acetylsalicylic acid (ASA, 300.0 mg/kg, p.o.) in hot-plate test, the examined compound 8 enhanced the antinociceptive activity in significant way. It also shows that rather the whole molecule is responsible for the antinociceptive and anti-inflammatory effect of the tested compound 8, however, it cannot be excluded that the summarizing effect is produced by ASA released from the compound 8 and the rest of triterpene derivative. The occurrence of tolerance for triterpenic derivative 8 was not observed, since the analgesic and anti-inflammatory effects after chronic administration of the conjugate OAO-ASA (8) was on the same level as after its single treatment. It seemed that the anti-inflammatory mechanism of action of OAO-ASA (8) is not simple, even its chronic administration lowered both blood concentration of IL-6 and mRNA IL-6 expression. However, the effects of the conjugate OAO-ASA (8) on TNF-α level and mRNA expression were opposite. Moreover, compound 8 did not change unequivocally mRNA TLR1, and TLR3 expression. Concluding, the obtained results regarding the antinociceptive and anti-inflammatory activity of new conjugate of oleanolic acid oxime and acetylsalicylic acid (OAO-ASA 8) are very interesting, but for explanation of its mechanism of action, more detailed studies are necessary.
- Published
- 2016
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34. Oleanolic Acid A-lactams Inhibit the Growth of HeLa, KB, MCF-7 and Hep-G2 Cancer Cell Lines at Micromolar Concentrations.
- Author
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Bednarczyk-Cwynar B, Ruszkowski P, Bobkiewicz-Kozlowska T, and Zaprutko L
- Subjects
- Antineoplastic Agents chemistry, Cell Line, Tumor, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Humans, Molecular Conformation, Oleanolic Acid chemistry, Structure-Activity Relationship, Antineoplastic Agents pharmacology, Lactams pharmacology, Oleanolic Acid pharmacology
- Abstract
Oleanolic acid ketones, oximes, lactams and nitriles were obtained. Complete spectral characterizations (IR, (1)H NMR, (13)C NMR, DEPT and MS) of the synthesized compounds are presented. The derivatives had oxo, hydroxyimino, lactam or nitrile functions at the C-3 position, an esterified or unmodified carboxyl group at the C- 17 location and, in some cases, an additional oxo function at the C-11 position. The new compounds were tested for cytotoxic activity on the HeLa, KB, MCF-7 and Hep-G2 cancer cell lines with the application of MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test. Among the tested compounds, some oximes and all lactams proved to be the most active cytotoxic agents. These triterpenes significantly inhibited the growth of the HeLa, KB, MCF-7 and Hep-G2 cancer cell lines at micromolar concentrations.
- Published
- 2016
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35. Advancing the delivery of anticancer drugs: Conjugated polymer/triterpenoid composite.
- Author
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Krukiewicz K, Jarosz T, Zak JK, Lapkowski M, Ruszkowski P, Bobkiewicz-Kozlowska T, and Bednarczyk-Cwynar B
- Subjects
- Antineoplastic Agents administration & dosage, Antineoplastic Agents chemistry, Delayed-Action Preparations administration & dosage, Diffusion, Materials Testing, Nanocapsules administration & dosage, Nanocapsules ultrastructure, Nanocomposites administration & dosage, Nanocomposites chemistry, Nanocomposites ultrastructure, Nanoconjugates administration & dosage, Nanoconjugates ultrastructure, Oleanolic Acid chemistry, Particle Size, Bridged Bicyclo Compounds, Heterocyclic chemistry, Delayed-Action Preparations chemistry, Nanocapsules chemistry, Nanoconjugates chemistry, Oleanolic Acid administration & dosage, Polymers chemistry, Triterpenes chemistry
- Abstract
Exemplifying the synergy of anticancer properties of triterpenoids and ion retention qualities of conjugated polymers, we propose a conducting matrix to be a reservoir of anticancer compounds. In this study, poly(3,4-ethylenedioxythiophene), PEDOT, based matrix for electrically triggered and local delivery of the ionic form of anticancer drug, oleanolic acid (HOL), has been investigated. An initial, one-step fabrication procedure has been proposed, providing layers exhibiting good drug release properties and biological activity. Investigation of obtained systems and implementation of modifications revealed another route of fabrication. This procedure was found to yield layers possessing a significantly greater storage capacity of OL(-), as evidenced by the 52% increase in the drug concentrations attainable through electro-assisted release. Examination of the biological activity of immobilised and released OL(-) molecules proved that electrochemical treatment has negligible impact on the anticancer properties of OL(-), particularly when employing the three-step procedure, in which the range of applied potentials is limited. PEDOT/OL(-) composite has been demonstrated to be a robust and cost-effective material for controlled drug delivery., (Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2015
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36. Simple amides of oleanolic acid as effective penetration enhancers.
- Author
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Bednarczyk-Cwynar B, Partyka D, and Zaprutko L
- Subjects
- Amides chemical synthesis, Amides chemistry, Membranes, Artificial, Ointments pharmacology, Oleanolic Acid chemistry, Permeability drug effects, Progesterone pharmacology, Amides pharmacology, Oleanolic Acid pharmacology, Skin Absorption drug effects
- Abstract
Transdermal transport is now becoming one of the most convenient and safe pathways for drug delivery. In some cases it is necessary to use skin penetration enhancers in order to allow for the transdermal transport of drugs that are otherwise insufficiently skin-permeable. A series of oleanolic acid amides as potential transdermal penetration enhancers was formed by multistep synthesis and the synthesis of all newly prepared compounds is presented. The synthetized amides of oleanolic acid were tested for their in vitro penetration promoter activity. The above activity was evaluated by means of using the Fürst method. The relationships between the chemical structure of the studied compounds and penetration activity are presented.
- Published
- 2015
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37. Recent advances in synthesis and biological activity of triterpenic acylated oximes.
- Author
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Bednarczyk-Cwynar B and Zaprutko L
- Abstract
During the last few decades more and more attention has been paid to triterpenes-a group of compounds with five- or four-ring skeleton and carboxyl, hydroxyl or oxo groups. Triterpenes with unsubstituted C-3 hydroxyl group can be easily transformed into appropriate ketones and then into oximes. The carbonyl group can be created not only from the hydroxyl group at C-3 position, but also at C-2, C-12 or C-28 positions. Several methods of creation of two = NOH groups within one molecule of triterpene are known. There are also known triterpenes with two carbonyl groups, e.g. at C-3 and C-11 positions, which differ in reactivity: among them only C-3 group can be transformed into oxime. A reactive hydroxyimine group can undergo the action of acylating agents, such as carboxylic acids or their derivatives, also the ones with significant pharmacological activity. Acyl derivatives of triterpenic oximes exhibit important pharmacological activity. The biological tests performed with the use of cell cultures inoculated with viruses showed inhibitory activity of some triterpenic acyloximes against type 1 HSV (H7N1), ECHO-6 and HIV-1 viruses. Another acylated oximes derived from triterpenes shown cytotoxic or antiproliferative activity against many lines of cancer cells. In many cases the pharmacological effects of the tested acyloxyiminotriterpenes were comparable to those of appropriate standard drugs. One of the newest application of acyl derivatives of triterpenic oximes is their ability to form organogels.
- Published
- 2015
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38. C-lactam derivatives of oleanolic acid. hydrolysis and further acylation of methyl acetyloleanolate C-lactam and C-thiolactam.
- Author
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Bednarczyk-Cwynar B and Zaprutko L
- Subjects
- Acylation, Hydrolysis, Lactams chemistry, Oleanolic Acid chemistry
- Abstract
Acetyl methyl oleanolate was transformed into a seven-membered C-lactam derivative (2) using Beckmann rearrangement of the corresponding C-oxime during the last step of the synthesis. The C=O group of the lactam system was transformed into a C=S group by Lavesson's reagent. The resulting acetylthiolactam 3 and initial acetyllactam 2 were subjected to alkaline hydrolysis to obtain lactam 4 and thiolactam 5 with an unsubstituted C-3 hydroxyl group. Subsequently, compounds 4 and 5 were acylated with either succinic or acetic anhydride in pyridine. Various acylating conditions were tested for hydroxythiolactam 5. The structures of the newly obtained compounds were supported by spectral and mass spectrometric data.
- Published
- 2014
39. Methyl 3-hydroxyimino-11-oxoolean-12-en-28-oate (HIMOXOL), a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MDA-MB-231 breast cancer cells.
- Author
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Lisiak N, Paszel-Jaworska A, Bednarczyk-Cwynar B, Zaprutko L, Kaczmarek M, and Rybczyńska M
- Subjects
- Apoptosis genetics, Apoptosis Regulatory Proteins genetics, Apoptosis Regulatory Proteins metabolism, Autophagy genetics, Beclin-1, Breast Neoplasms drug therapy, Breast Neoplasms genetics, Breast Neoplasms metabolism, Caspase 3 genetics, Caspase 3 metabolism, Caspase 8 genetics, Caspase 8 metabolism, Cell Cycle drug effects, Cell Cycle genetics, Cell Death drug effects, Cell Death genetics, Cell Line, Tumor, Cell Survival drug effects, Cell Survival genetics, Female, Humans, Membrane Proteins genetics, Membrane Proteins metabolism, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Mitogen-Activated Protein Kinases genetics, Mitogen-Activated Protein Kinases metabolism, NF-kappa B genetics, NF-kappa B metabolism, Oleanolic Acid pharmacology, Poly (ADP-Ribose) Polymerase-1, Poly(ADP-ribose) Polymerases genetics, Poly(ADP-ribose) Polymerases metabolism, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, Signal Transduction drug effects, Signal Transduction genetics, Up-Regulation drug effects, Up-Regulation genetics, bcl-2-Associated X Protein genetics, bcl-2-Associated X Protein metabolism, Antineoplastic Agents pharmacology, Apoptosis drug effects, Autophagy drug effects, Oleanolic Acid analogs & derivatives
- Abstract
HIMOXOL (methyl 3-hydroxyimino-11-oxoolean-12-en-28-oate) is a synthetic derivative of oleanolic acid (OA). HIMOXOL revealed the highest cytotoxic effect among tested synthetic OA analogs. In this study we focused on elucidating the cytotoxic mechanism of HIMOXOL in MDA-MB-231 breast cancer cells. HIMOXOL reduced MDA-MB-231 cell viability with an IC50 value of 21.08±0.24μM. In contrast to OA, the tested compound induced cell death by activating apoptosis and the autophagy pathways. More specifically, we found that HIMOXOL was able to activate the extrinsic apoptotic pathway, which was proven by observation of caspase-8, caspase-3 and PARP-1 protein activation in Western blot analysis. An increase in the ratio of Bax/Bcl-2 protein levels was also detected. Moreover, HIMOXOL triggered microtubule-associated protein LC3-II expression and upregulated beclin 1. This observed compound activity was modulated by mitogen-activated protein kinases and NFκB/p53 signaling pathways. Together, these data suggest that HIMOXOL, a synthetic oleanolic acid derivative which activates dual cell death machineries, could be a potential and novel chemotherapeutic agent., (Copyright © 2013. Published by Elsevier Ireland Ltd.)
- Published
- 2014
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40. Targeting nrf2-mediated gene transcription by triterpenoids and their derivatives.
- Author
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Loboda A, Rojczyk-Golebiewska E, Bednarczyk-Cwynar B, Lucjusz Z, Jozkowicz A, and Dulak J
- Abstract
Chemoprevention represents a strategy designed to protect cells or tissues against various carcinogens and carcinogenic metabolites derived from exogenous or endogenous sources. Recent studies indicate that plant-derived triterpenoids, like oleanolic acid, may exert cytoprotective functions via regulation of the activity of different transcription factors. The chemopreventive effects may be mediated through induction of the nuclear factor erythroid 2-related factor 2 (Nrf2) transcription factor. Activation of Nrf2 by triterpenoids induces the expression of phase 2 detoxifying and antioxidant enzymes such as NAD(P)H quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1) - proteins which can protect cells or tissues against various toxic metabolites. On the other hand, inhibition of other transcription factors, like NF-κB leads to the decrease in the pro-inflammatory gene expression. Moreover, the modulation of microRNAs activity may constitute a new mechanism responsible for valuable effects of triterpenoids. Recently, based on the structure of naturally occurring triterpenoids and with involvement of bioinformatics and computational chemistry, many synthetic analogs with improved biological properties have been obtained. Data from in vitro and in vivo experiments strongly suggest synthetic derivatives as promising candidates in the chemopreventive and chemotherapeutic strategies.
- Published
- 2012
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41. The analgesic and anti-inflammatory effect of new oleanolic acid acyloxyimino derivative.
- Author
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Bednarczyk-Cwynar B, Zaprutko L, Marciniak J, Lewandowski G, Szulc M, Kaminska E, Wachowiak N, and Mikolajczak PL
- Subjects
- Analgesics chemical synthesis, Analgesics pharmacology, Animals, Anti-Inflammatory Agents chemical synthesis, Anti-Inflammatory Agents pharmacology, Carrageenan, Drug Synergism, Edema chemically induced, Edema drug therapy, Male, Mice, Morphine pharmacology, Motor Activity drug effects, Oleanolic Acid chemical synthesis, Oleanolic Acid pharmacology, Oleanolic Acid therapeutic use, Rats, Rats, Wistar, Toxicity Tests, Acute, Analgesics therapeutic use, Anti-Inflammatory Agents therapeutic use, Drug Eruptions drug therapy, Oleanolic Acid analogs & derivatives, Pain drug therapy
- Abstract
The new derivative of well-known triterpene, oleanolic acid: methyl 3-octanoyloxyiminoolean-12-en-28-oate 5, was synthesized by the action of caprylic acid on methyl oleanolate 3-oxime in the presence of dicyclohexylcarbodiimide in dioxane. The molecular structure of the obtained product 5 was confirmed by spectral methods. The acute toxicity, locomotor activity, and the dose-dependent analgesic activity were studied. In addition, the effect of compound 5 on morphine-induced analgesic activity, the dose-dependent anti-inflammatory activity and the effect of the compound on diclofenac anti-inflammatory activity study were performed. The results proved a low toxicity (LD₅₀ > 2 g/kg) of the tested product 5, which affected neither vertical nor horizontal locomotor activity in the given range of doses. The triterpene 5 also produced centrally mediated (morphine-like) analgesic action; however, only in the highest dose. The synergistic analgesic activity of 5 and morphine in the doses of 30.0 and 300.0mg/kg was found. Compound 5 expressed the anti-inflammatory action which did not affect the anti-inflammatory activity of diclofenac after their combined administration., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
42. Anilides and toluidides of 3beta-acetyloleanolic acid.
- Author
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Bednarczyk-Cwynar B
- Subjects
- Molecular Structure, Anilides chemical synthesis, Oleanolic Acid chemistry, Triterpenes chemistry
- Abstract
Amide formation is one of the reactions that can be undertaken within the carboxyl group of oleanolic acid. A simple method for oleanolic acid anilide and toluidides synthesis is presented. The influence of the location of the methyl substituent on the reactivity of the amine group was tested and the "ortho effect" of the methyl substituent within the molecule of o-toluidine on the time of reaction was observed. The structures of the newly obtained compounds were determined by spectroscopic methods.
- Published
- 2012
43. Anticancer effect of A-ring or/and C-ring modified oleanolic acid derivatives on KB, MCF-7 and HeLa cell lines.
- Author
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Bednarczyk-Cwynar B, Zaprutko L, Ruszkowski P, and Hładoń B
- Subjects
- Antineoplastic Agents pharmacology, Cell Line, Tumor, Cell Survival drug effects, HeLa Cells, Humans, Molecular Structure, Oleanolic Acid pharmacology, Structure-Activity Relationship, Antineoplastic Agents chemistry, Oleanolic Acid analogs & derivatives
- Abstract
New A-ring or/and C-ring modified methyl oleanolate derivatives were prepared. New simple method of synthesis of 3,12-diketone (3) from methyl oleanonate (2) was worked out. The obtained new compounds were tested for cytotoxic activity on KB, MCF-7 and HeLa cell lines. The derivatives had acetoxy, oxo or hydroxyimino function at the C-3 position and in some cases oxo, hydroxyimino or acyloxyimino group at the C-12 position. Almost all of the compounds showed strong cytotoxic activity, higher than unchanged oleanolic acid. The most active substances turned out to be the derivatives with acyloxyimino function, especially 4 and 8d.
- Published
- 2012
- Full Text
- View/download PDF
44. C-lactam derivatives of oleanolic acid. The synthesis of C-lactam by Beckmann rearrangement of C-oxime.
- Author
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Bednarczyk-Cwynar B
- Subjects
- Lactams chemical synthesis, Oleanolic Acid analogs & derivatives, Oximes chemistry
- Abstract
Oleanolic acid, one of the most known triterpenes, was subjected to different chemical transformations within C-3 beta-hydroxyl group, a double bond between C-12 and C-13, and a carboxyl function at C-17 in order to obtain new derivatives. The key compound consists of four six-membered rings (A, B, D, E) and one enlarged ring (C ring) containing a nitrogen atom and a carbonyl function - lactam. This type of derivative can be obtained by Beckmann rearrangement of the appropriate oxime. The lactam can be transformed into thiolactam with the use of Lavesson's reagent. The method is also presented for new derivatives synthesis, as well as their structure elucidation by spectroscopic means.
- Published
- 2011
45. 3β-Acet-oxy-12α-chloro-d-friedooleanan-28,14β-olide.
- Author
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Froelich A, Kowiel M, Bednarczyk-Cwynar B, Zaprutko L, and Gzella AK
- Abstract
The title compound, C(32)H(49)ClO(4), was obtained along with nitrile and lactam products in the POCl(3)-catalysed Beckmann rearrangement from 3β-acet-oxy-12-hydroxyiminoolean-28-olic acid methyl ester. The mechanism of the transformation leading to the title compound remains unclear and requires further investigation. Rings A, B and E are in chair conformations, ring C has a twisted-boat conformation, ring D a conformation halfway between boat and twisted-boat and rings D and E are cis-fused. In the crystal, mol-ecules are connected by weak inter-molecular C-H⋯O hydrogen bonds into layers extending parallel to the bc plane.
- Published
- 2011
- Full Text
- View/download PDF
46. Oleanolic acid derivative methyl 3,11-dioxoolean-12-en-28-olate targets multidrug resistance related to ABCB1.
- Author
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Paszel A, Rubiś B, Bednarczyk-Cwynar B, Zaprutko L, Kaczmarek M, Hofmann J, and Rybczyńska M
- Subjects
- ATP Binding Cassette Transporter, Subfamily B, ATP Binding Cassette Transporter, Subfamily B, Member 1 physiology, Cell Line, Tumor, Cell Survival drug effects, Cell Survival physiology, Dose-Response Relationship, Drug, Drug Resistance, Multiple physiology, Drug Resistance, Neoplasm physiology, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1 antagonists & inhibitors, Dioxolanes administration & dosage, Drug Delivery Systems methods, Drug Resistance, Multiple drug effects, Drug Resistance, Neoplasm drug effects, Oleanolic Acid administration & dosage, Oleanolic Acid analogs & derivatives
- Abstract
Multidrug resistance (MDR) in leukemia patients is a great incentive to the development of new drugs. In a search for potential multidrug resistance modulators we tested a group of oleanolic acid (OA) analogues modified at C-3, C-11, C-12 and C-28 using an experimental model consisting of three human acute lymphoblastic leukemia cell lines (CCRF-CEM and the multidrug resistant sublines CCRF-VCR1000 and CCRF-ADR5000). The most effective compound, methyl 3,11-dioxoolean-12-en-28-olate (DIOXOL) was more potent in cell viability inhibition than its precursor - OA, and showed similar or even higher activity in the drug resistant than in the wild-type cells. Resistance factor (RF) values obtained for CCRF-VCR1000 and CCRF-ADR-5000 cells using MTT assay were 0.7 and 0.8 (24 h of treatment) and after 72 h of treatment 0.9 and 1.1, respectively. Moreover, 5 μM DIOXOL significantly reduced the expression of the ABCB1 gene in MDR cells by around 30%, and also decreased the level of P-gp protein. Compared to untreated control cells, DIOXOL treatment resulted in a significant P-gp decrease (30% in CCRF-ADR5000 and 50% in CCRF-VCR1000), that was detected by western blot and confirmed by flow cytometry analysis. Moreover, DIOXOL (at 10 μM) significantly inhibited P-gp transport function by more than twofold comparing to control, untreated cells that was demonstrated using rhodamine 123-based functional test. The compound exhibited synergistic activity with ABCB1 substrate - adriamycin in CCRF-VCR1000 cells, indicating partial but significant MDR reversing ability.
- Published
- 2011
- Full Text
- View/download PDF
47. Triterpenoids. Part 21: Oleanolic acid azaderivatives as percutaneous transport promoters.
- Author
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Zaprutko L, Partyka D, and Bednarczyk-Cwynar B
- Subjects
- Adjuvants, Pharmaceutic chemical synthesis, Administration, Cutaneous, Aza Compounds chemical synthesis, Biological Transport, Active, Humans, In Vitro Techniques, Lactams chemical synthesis, Permeability, Progesterone chemistry, Structure-Activity Relationship, Triterpenes chemical synthesis, Adjuvants, Pharmaceutic chemistry, Aza Compounds chemistry, Lactams chemistry, Membranes, Artificial, Skin Absorption, Triterpenes chemistry
- Abstract
Some new oleanolic acid derivatives with lactame and thiolactame structures in the A- or C-ring were prepared and tested as percutaneous transport promoters in vitro. Their activity was comparable with activity of N-dodecylcaprolactame (Azone). A-Thiolactame derivative of methyl oleanolate (13) was the most effective compound.
- Published
- 2004
- Full Text
- View/download PDF
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