1. Characterization of a novel inulosucrase from Lactiplantibacillus plantarum.
- Author
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Sarang S, Ernst L, Wefers D, and Kulkarni R
- Subjects
- Hydrogen-Ion Concentration, Temperature, Enzyme Stability, Molecular Weight, Lactobacillaceae enzymology, Lactobacillaceae genetics, Lactobacillaceae metabolism, Lactobacillaceae chemistry, Hexosyltransferases genetics, Hexosyltransferases metabolism, Hexosyltransferases chemistry, Bacterial Proteins genetics, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Inulin chemistry, Inulin metabolism
- Abstract
Fructansucrases produce fructans by polymerizing the fructose moiety released from sucrose. Here, we describe the recombinant expression and characterization of a unique fructansucrase from Lactiplantibacillus plantarum DKL3 that showed low sequence similarity with previously characterized fructansucrases. The optimum pH and temperature of fructansucrase were found to be 4.0 and 35 °C, respectively. Enzyme activity increased in presence of Ca
2+ and distinctly in presence of Mn2+ . The enzyme was characterized as an inulosucrase (LpInu), based on the production of an inulin-type fructan as assessed byNMR spectroscopy and methylation analysis. In addition to β-2,1-linkages, the inulin contained a few β-2,1,6-linked branchpoints. High-performance size exclusion chromatography with refractive index detection (HPSEC-RI) revealed the production of inulin with a lower molecular weight compared to other characterized bacterial inulin. LpInu and its inulin product represent novel candidates to be explored for possible food and biomedical applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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