1. Preparation and identification of an anti-idiotypic antibody antagonist (FG8) for EGFR that shows potential activity against liver cancer cells.
- Author
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Wang Y, He F, Zhang H, Cao Y, Zhang Y, Ling Y, and Rehati A
- Subjects
- Antibodies, Anti-Idiotypic drug effects, Antibodies, Anti-Idiotypic immunology, Antineoplastic Agents chemistry, Apoptosis drug effects, Binding, Competitive drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Enzyme-Linked Immunosorbent Assay, Epitopes drug effects, Epitopes genetics, ErbB Receptors antagonists & inhibitors, ErbB Receptors genetics, ErbB Receptors immunology, Humans, Liver Neoplasms genetics, Liver Neoplasms immunology, Liver Neoplasms pathology, Protein Binding genetics, Signal Transduction drug effects, Antibodies, Anti-Idiotypic biosynthesis, Antineoplastic Agents pharmacology, Liver Neoplasms drug therapy
- Abstract
Objective: Currently, there are two categories of epidermal growth factor receptor (EGFR) antagonists, small molecule antagonists and anti-EGFR antibodies. In the current study, we developed a new EGFR antagonist employing the anti-idiotypic antibodies strategy., Results: First, using EGF as an antigen, through a series of immunological protocols and hybridoma technology, we obtained an anti-idiotypic antibody against EGF receptor-binding epitopes. On this basis, we screened and characterized the anti-idiotype antibodies against EGFR through competitive ELISA, co-localization analysis, competitive receptor binding analysis, and immunofluorescence. Finally, an internal image anti-idiotype antibody called FG8 was successfully prepared. Experiment result shows that FG8 inhibits EGFR-mediated signaling pathways in vitro. Additionally, FG8 inhibits liver tumor cell proliferation as well as induces tumor cell apoptosis., Conclusions: The present study suggests that FG8 is a potential therapeutic agent for liver cancer. In addition, this study provides a novel method for the preparation of EGFR antagonists.
- Published
- 2021
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