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Immobilization of antibodies on alginate-chitosan beads.
- Source :
-
International journal of pharmaceutics [Int J Pharm] 2000 Sep 25; Vol. 206 (1-2), pp. 23-34. - Publication Year :
- 2000
-
Abstract
- An anti-hapten IgG was covalently immobilized on glutaraldehyde-activated alginate-chitosan gel beads. The antibody immobilization efficiency was influenced by glutaraldehyde-bead reaction time, IgG concentration and pH. In addition, immobilization conditions such as glutaraldehyde and antibody concentrations influenced antibody hapten binding affinity. The immobilized IgG on the beads was stable and no reduction in the percent binding to hapten was noticed following 25 days of storage. It was concluded that antibodies could be successfully immobilized on alginate-chitosan gel beads. Such a system can be applied for the development of immunoaffinity purification and immunoassays.
- Subjects :
- Animals
Antibodies, Anti-Idiotypic drug effects
Binding Sites, Antibody drug effects
Cells, Immobilized metabolism
Chitin metabolism
Chitosan
Fixatives pharmacology
Gels
Glutaral pharmacology
Hydrogen-Ion Concentration
Immunoglobulin G drug effects
Rabbits
Alginates metabolism
Antibodies, Anti-Idiotypic metabolism
Biocompatible Materials metabolism
Chitin analogs & derivatives
Immunoglobulin G metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0378-5173
- Volume :
- 206
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- International journal of pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 11058807
- Full Text :
- https://doi.org/10.1016/s0378-5173(00)00470-1