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Olive pollen allergy: searching for immunodominant T-cell epitopes on the Ole e 1 molecule.
- Source :
-
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 1998 Apr; Vol. 28 (4), pp. 413-22. - Publication Year :
- 1998
-
Abstract
- Background: The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) has been described and the IgE antibody response to this major allergen was associated with DR7/DQ2 antigens. With this previous data we try to define the T-cell epitopes implicated in Ole e 1 reactivity.<br />Objectives: To study the recognition of T cells (derived from allergic and non-allergic Ole e 1 patients) to Ole e 1 synthetic peptides in order to define immunodominant T-cell epitopes.<br />Methods: We have compared the proliferative response of the peripheral blood mononuclear cells from Ole e 1 sensitized patients vs. non-sensitized controls, induced by 14 Ole e 1 synthetic peptides. Thirty subjects were classified in two groups: group 1 (non-responders against Ole e 1, n=16) and group 2 (Ole e 1 responders, n=14), according to their clinical parameters and the presence or not in their sera of the significant Ole e 1 IgE antibody levels.<br />Results: Our results shown that it is possible to find T cells reactive to Ole e 1 peptides in patients with and without significant levels of Ole e 1 IgE antibodies. However, the percentage of response was higher in patients with IgE antibodies 71.4% vs 25%), and the recognition profile was different: the control group showed a broad reactivity pattern, in contrast, the response by the 'Ole e 1 responders' group was mainly directed against three peptides of the carboxi-terminal region, peptides 10 (91-102), 12 (109-120) and 13 (119-130), with a response frequency of 35.7, 28.5 and 28.5%, respectively. By direct and inhibition test no antibody response was found against the synthetic peptides.<br />Conclusions: Our data suggest that the regions between 91 and 102 and 109-130 aminoacids on the Ole e 1 molecule are immunodominant T-cell epitopes. These epitopes are not recognized by IgE antibodies.
- Subjects :
- Adolescent
Adult
Amino Acid Sequence
Antibodies, Anti-Idiotypic blood
Antibodies, Anti-Idiotypic drug effects
Antibody Formation drug effects
Antibody Formation immunology
Antigens, Plant
Cell Division drug effects
Cell Division immunology
Epitopes chemistry
Epitopes immunology
Epitopes pharmacology
Female
HLA-D Antigens blood
Humans
Hypersensitivity blood
Immunoglobulin E blood
Immunoglobulin E drug effects
Immunoglobulin G blood
Immunoglobulin G drug effects
Leukocytes, Mononuclear cytology
Leukocytes, Mononuclear drug effects
Leukocytes, Mononuclear immunology
Male
Middle Aged
Molecular Sequence Data
Oligopeptides immunology
Oligopeptides pharmacology
Plant Proteins chemistry
Plant Proteins immunology
Plant Proteins pharmacology
Pollen adverse effects
Pollen chemistry
T-Lymphocytes chemistry
T-Lymphocytes immunology
Trees chemistry
Allergens
Hypersensitivity immunology
Pollen immunology
Trees immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0954-7894
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 9641567
- Full Text :
- https://doi.org/10.1046/j.1365-2222.1998.00190.x