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Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The skin and fur condition is an important indicator judging the growth of animals. Healthy animals always exhibit characteristics of rosy skin and oily hair. The metabolic pathway involved in vitamins plays key roles in the physiological processes of growth, development and reproduction of animals. Particularly, vitamin nutrition directly affects the fur condition of animals. Vitamin A and its derivatives can regulate the exfoliation of dead cells in the stratum corneum and the proliferation of living cells in the epidermis, promoting skin cell renewal and increasing skin elasticity. Vitamin C can eliminate free radicals, reduce oxidative stress damage to the skin, and participate in collagen synthesis and repair by regulating the activity of skin proline and lysine hydroxylase. Vitamin D can regulate the differentiation and proliferation of the epidermis by maintaining normal skin metabolism and promoting hair growth, forming a stable barrier to prevent water loss and the invasion of microorganisms. Vitamin E can prevent lipid oxidation of skin cell membranes and maintain the integrity and stability of cell membranes. B vitamins mainly serve as coenzymes and have regulatory effects on skin elasticity, skin immunity, fur color, and smoothness. Although vitamins are relatively low in feed formulations, they play an important role in animal fur development and other physiological functions. This article reviews the effects and mechanisms of vitamins on animal fur, in order to provide a reference for regulation strategies of animal fur condition. [ABSTRACT FROM AUTHOR]

In order to further standardize the diagnosis and treatment of long-term systemic complications in kidney transplant recipients, Branch of Organ Transplantation of Chinese Medical Association initiated the formulation of “Clinical Diagnosis and Treatment Guidelines for Long-term Systemic Complications in Kidney Transplant Recipients in China”. Experts on organ transplantation were organized to summarize and integrate the latest progress in this field based on existing clinical guidelines, systematic evaluations, case studies, expert consensus. The guideline was formed after multiple rounds of discussion and reaching a consensus which included complications of hematological system, central nervous system, cardiovascular system, ocular, cutaneous and osteoporosis disorders. The full text focuses on 27 clinical problems and forms 40 recommendations, mainly involving the risk factors, classification, diagnosis, treatment and prevention of various complications. This guideline graded the quality of evidence and the strength of recommendation for each clinical issue using 2009 Oxford Centre for Evidence-Based Medicine (OCEBM) Grading and Strength of Recommendation criteria, so as to provide reference for the diagnosis and treatment of late complications, comprehensively improve the management capacity of clinicians to benefit kidney transplant recipients. [ABSTRACT FROM AUTHOR]

BACKGROUND: Minipigs are often used in research fields such as skin injury, vascular trauma and cosmetic medicine because they are highly similar to human beings in terms of skin tissue structure and cardiovascular system. Hydrogel as a wound repair drug possesses a variety of excellent physicochemical properties such as strong water retention and adhesion, which can provide isolation moisturization and drug release for wounds. OBJECTIVE: To summarize and conclude the progress of the application of trauma models for different experimental purposes of hydrogel therapy for minipigs, to reveal the development status of various types of minipig trauma models, to analyze the deficiencies of minipig trauma models at the present stage. METHODS: The relevant articles published in Web of Science database and CNKI database from the establishment of each database to 2023 were checked, using “piglet, miniature pig, minipig, miniature pig; gel, hydrogel; trauma, injury, wound, lesion, incision” as Chinese search terms and “Miniature Swine, Miniature pig, minipig; gel, hydrogel; injury, wound, lesion, incision” as English search terms. A total of 438 Chinese and English documents were retrieved, and 59 documents were included in the study through the inclusion and exclusion criteria. RESULTS AND CONCLUSION: (1) At present, the main models used clinically for trauma repair are large animal species (dogs and pigs), rabbits, and rodents (rats and mice). Because the skin structure of the minipig is more like that of humans, the minipig is the most ideal animal model for trauma repair. (2) In the invitro skin injury model, skin defect model is the basic wound model, which can be divided into full skin defect model and medium-thickness skin defect model according to the depth of the wound defect. Burn wound model and infected wound model are multidimensional models with hot metal scald and bacterial culture imposed on the basis of the skin defect model, which have the advantages of high safety coefficient and low operation difficulty. (3) In the in-vivo trauma repair model, mini-pigs are used as esophageal cricothyrotomy model which is more in line with the pathological state of clinical diseases. Mini-pigs are used in the gastric perforation and vascular hemostasis model, which can visually demonstrate the stronger organ adhesion, hemostatic properties and tissue regeneration-promoting effects of the hydrogel. (4) The specific parts of the pig also has the corresponding mode of use: pig ear is usually used to evaluate the hydrogel drug delayed-release effect. Porcine cellular proteins and pig skin collagen are mostly used to prepare composite hydrogels of tissue origin. [ABSTRACT FROM AUTHOR]

The behaviors of biomaterials are influenced by a multitude of factors including temperature, mechanical load, and biochemical environments. The dynamic equilibrium of organism within a multiphysical field is crucial for its physiological functions. Therefore, a comprehensive investigation into the bio-thermo-mechano- electrophysiological behaviors is of great significance for the elucidation of pathologies and the development of effective diagnostic and therapeutic strategies. Such inquiries inherently require a multidisciplinary approach, necessitating the integration of diverse disciplinary knowledge and the pioneering of innovative cross-disciplinary research. The core idea is to discover key scientific questions, identify interdisciplinary disciplines, integrate disciplinary advantages, promote theoretical and technological innovations, and bring new breakthroughs in the biomedical field. With the discovery of DNA double helix structure in Cambridge University as the background, and the tissues of brain, skin, and teeth, etc. as examples, this paper introduces the evolution and intension of bio-thermo-mechano-electrophysiology, a new interdisciplinary subject [ABSTRACT FROM AUTHOR]

BACKGROUND: Currently, a variety of mesenchymal stem cells have been confirmed to have the effect of promoting wound repair, but there is still a lack of relevant research on whether placenta-derived mesenchymal stem cells can promote acute skin wound healing. OBJECTIVE: To investigate the effect of placenta-derived mesenchymal stem cell transplantation on the healing of acute skin wound in rats. METHODS: Twenty SD rats were divided into PBS group and stem cell group by the random number table method, with 10 rats in each group. All rats were selected to establish a full-thickness skin defect model. In the PBS group and stem cell group, PBS buffer and placenta-derived mesenchymal stem cells were immediately injected on the wound surface and wound margin immediately and on day 8 after modeling. The wound healing was observed immediately and on days 2, 4, 6, 8, 10, 12, and 14 after modeling. The skin tissue of the wound surface was taken on day 14 and treated with hematoxylin-eosin staining, Masson staining, immunohistochemical staining and immunofluorescence staining. RESULTS AND CONCLUSION: (1) The wound surface of the rats in each group decreased with the prolongation of treatment time. The wound healing rate and wound epithelization rate of the stem cell group at 14 days were higher than those of the PBS group (P < 0.01), and the wound contracture rate was lower than that of the PBS group (P < 0.01). (2) The results of hematoxylin-eosin staining showed that the skin wound healing of the stem cell group was better than that of the PBS group; the degree of wound epithelization was higher, and the morphology of collagen fibers was close to that of normal skin. (3) Masson staining results showed that compared with the PBS group, collagen fibers in the skin wound tissue of the stem cell group were significantly increased and thicker, and the content of collagen fibers in the new tissue was significantly higher than that of the PBS group (P < 0.01). (4) Immunohistochemical staining showed that the number of new capillaries in the stem cell group was higher than that in the PBS group (P < 0.01), while the expressions of tumor necrosis factor-α and interleukin-6 were lower than those in the PBS group (P < 0.01). (5) Immunofluorescence staining showed that the number of M2 macrophages in the new wounds of the stem cell group was higher than that of the PBS group (P < 0.01), while the number of M1 macrophages was less than that in the PBS group (P < 0.01). These findings indicate that placenta-derived mesenchymal stem cells can accelerate skin wound healing, promote wound epithelization, and reduce wound contracture, which may be related to the promotion of capillary angiogenesis, regulation of collagen fiber production, inhibition of inflammation, and regulation of macrophage polarization to M2 type. [ABSTRACT FROM AUTHOR]

The skin, being a highly intricate organ within the human body, has long posed a challenging and significant aspect in both clinical and research domains when constructing a bionic skin model. 3D bioprinting technology has effectively addressed this issue by facilitating the creation of skin constructs. This innovative technique allows for the rapid and dependable production of bionic skin substitutes, as it enables the customization of skin shapes and the precise distribution of cells and other materials to meet the specific demands of clinical and industrial applications. Furthermore, this technology exhibits exceptional performance, adaptability, superior resolution, reproducibility, and high throughput, rendering it suitable for diverse applications such as drug screening, cosmetic testing, and skin grafts. Consequently, it holds the potential to serve as a viable skin substitute for a larger population of individuals afflicted with burns or diabetes. The integration of 3D bioprinting technology with stem cells has provided researchers and medical professionals with increased opportunities to address skin diseases. Pluripotent stem cells are particularly promising due to their ethical acceptability and ability to be genetically customized to meet individual patient’s needs. This customization enables the development of cell carriers with predetermined structures, which can incorporate biomimetic hierarchies, including blood vessel networks, hair follicles, and sweat glands. These advancements aim to enhance tissue functionality and improve the aesthetic outcomes of implantation procedures. This article summarizes the current research progress in skin construction using 3D bioprinting technology. It specifically highlights the distinctive features of skin construction achieved through various 3D bioprinting techniques. Additionally, it analyzes the specific requirements for skin construction that includes appendages, aiming to offer valuable insights for the development of in vitro physiological or pathological models, regenerative medicine, and clinical research within this domain. [ABSTRACT FROM AUTHOR]

By collecting a large number of corpus, the author analyzes the meaning evolution of “皮(pi)”, “肤(fu)”and“皮肤(pifu)”. The results of synchronic deduction and diachronic verification show that“皮肤 (pifu)" does not evolve from a phrase to a word, but from the synonymous juxtaposition of the monosyllabic word “皮(pi)”and“肤(fu)” in ancient Chinese. Promoted by frequent usage, prosodic constraints, syntactic posi- tion and the objective lexicalization motivation of synonymous categories,“皮肤(pifu)” has been lexicalized through subjective psychological cognitive mechanisms. [ABSTRACT FROM AUTHOR]

There was a 61-year-old male patient with cutaneous metastatic lung adenocarcinoma inoculated along a thoracic drainage tube treated in Zhongshan Hospital, Fudan University. The duration of disease was over 2 weeks. The skin lesions were extensive and appeared as purplish red nodules ranging in size from mung bean to small walnut. The nodules were tough and solid, smooth in surface, with infiltration while without ulceration, pruritus or tenderness. The patient developed chest tightness and pain without obvious causes, dull pain in nature and less white sputum 6 weeks before the eruption, and then was diagnosed as malignant tumors of the left lung (poorly differentiated adenocarcinoma), cT1cN3M1c stage Ⅳ B (pleura, bone). Gene tests showed that epidermal growth factor receptor(EGFR)21 exon point mutation L858r，Ros1(-), anaplastic lymphoma kinase (ALK)(-), and physical status score (PS) of 0. The treatment was Dactinib 45mg once each day, supplemented the bone preservation therapy by ibandronic acid. As the patient's condition did not significantly improve and skin nodules increased, also the abdominal B-ultrasound examination showed that multiple metastases in liver, the treatment was changed to Ectinib hydrochloride tablets (Kemena) of 125 mg 3 times a day, combined with Crizotinib capsules (Secor) of 200 mg twice a day. Unfortunately, the patient has now been lost to follow-up. [ABSTRACT FROM AUTHOR]

Alopecia areata (AA) is a common autoimmune hair loss disease. Under the guidance of Traditional Chinese medicine (TCM) syndrome differentiation theory and combined with collateral disease theory, this article summarized the etiology, pathogenesis, clinical characteristics, treatment principles, treatment methods, and prescriptions of AA by stages. In this article, three new etiology and pathogenesis have been summarized as the pathogenic Qi invades the collaterals, the stagnation of Qi and blood in the collaterals, and the deficiency of the collaterals. The treatment principle of AA is mainly refined into three stages, which include clearing heat, removing dampness, and harmonizing the collaterals for the initial stage; invigorating Qi, cultivating blood, and dredging collaterals for the middle stage; supplementing the liver, nourishing the kidney and enrich the collaterals for the terminal stage. The main ways of treatment include oral and external application of Chinese medicine and TCM external therapy. (斑秃是一种常见自身免疫性脱发。在中医辨证理论指导下, 结合络病学理论, 本文探讨斑秃各期的病因病机、临床特点及治则治法方药等。斑秃的病机可总结为盛邪袭络、气血凝络、络脉亏虚, 治疗原则以初期清热除湿和络、中期益气养血通络、末期养肝益肾补络为主, 采用中药口服、外用及中医外治手法进行治疗。)

Copyright of Detergent & Cosmetics is the property of Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (2097-2806) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Mesenchymal stem cell transplantation has proven to be a promising new therapy in the treatment of chronic wounds. In terms of enhancing the efficacy, there are few reports on the different delivery routes of mesenchymal stem cells and the comparison of their advantages and disadvantages. Optimizing the delivery route is particularly important for improving the application effect of mesenchymal stem cells in wound repair. OBJECTIVE: To discuss the relevant mechanisms of mesenchymal stem cells in promoting wound repair, and summarize the application effects of different transplantation methods in chronic wound repair. METHODS: The first author retrieved the relevant documents included in CNKI Database, Wanfang Database and PubMed database. The Chinese search terms were “mesenchymal stem cells, mechanism, transplantation, pathway, chronic wound, vulnus, regeneration, tissue engineering, skin, ulcer”. The English search terms were “stem cell/stromal cells/progenitor cell/mesenchymal stem cells, mechanism/therapeutic application, transplantation route/deliver/engraftment/injection/infusion/drug administration routes, wound healing/chronic wound/regeneration/tissue engineering/tissue repair/skin ulce”. The retrieval time of the literature was limited to January 2000-July 2021. The literature was initially screened by reading the abstract of the article, and the literature that was not related to the subject of the article was excluded, and the number of articles finally included was 85 for review. RESULTS AND CONCLUSION: (1) The paracrine function is the main mechanism by which mesenchymal stem cells play the role of wound repair. Mesenchymal stem cells promote angiogenesis by secreting soluble factors and releasing extracellular vesicles, reduce local inflammatory response, regulate the remodeling of extracellular matrix, and rebuild the microenvironment of tissue regeneration. (2) In summary, biomaterial scaffolds are one of the most promising transplantation methods for mesenchymal stem cells. Compared with traditional systemic administration (arterial administration and intravenous administration) and traditional local administration (such as subcutaneous and intramuscular injection), excellent plasticity, bioactivity, biodegradation and biocompatibility are the four key characteristics of their advantages. It overcomes many problems of poor safety and low efficiency of traditional drug delivery routes, which improves the application effect of mesenchymal stem cells in chronic wounds, and its advantages need to be further developed. (3) The choice of transplantation pathway depends on its therapeutic purpose and mechanism to a certain extent. (4) As for the relationship between the action mechanism of mesenchymal stem cells and the route of administration, the homing of mesenchymal stem cells to the damaged tissue was taken as an example. When the transplantation mode of mesenchymal stem cells is local administration (such as intramuscular), in addition to the basic interstitial migration of mesenchymal stem cells in the damaged tissue, the homing and migration of mesenchymal stem cells are not necessary. If the transportation of mesenchymal stem cells is holistic (such as arterial pathway and venous pathway), the homing mechanism of mesenchymal stem cells is necessary to achieve its repair goal. Therefore, to improve the application quality, the route of administration and the mechanism of mesenchymal stem cells participating in wound repair should be considered comprehensively. [ABSTRACT FROM AUTHOR]

Peripheral neuropathy is a common complication during treatment of multiple myeloma, which affecting the prognosis and patients’ quality of life. Effective prevention, treatment and nursing interventions have been proved to reduce the incidence of multiple myeloma complicated by peripheral neuropathy. This paper reviewed the studies on nursing of multiple myeloma complicated by peripheral neuropathy in perspectives of risk factors, prevention, treatment and nursing interventions, in order to provide reference for clinical nursing practice. (多发性骨髓瘤周围神经病变(MMPN)是多发性骨髓瘤(MM)治疗过程中常见的并发症之一, 严重影响患者的生活质量及预后, 有效的预防措施、治疗方法和护理干预均能有效降低MMPN的发生率。本研究从MMPN的危险因素、预防、治疗及护理干预措施进行综述, 旨在为MMPN患者的护理提供参考依据和实践指导。)

Hematopoietic stem cell transplantation (HSCT) is an important method for the treatment of malignant and non-malignant refractory hematologic diseases. Graft-versus-host disease (GVHD) is one of the main complications, which seriously affects the prognosis of patients. By reviewing of related studies, this paper summarized pathogenesis, clinical classification, prevention and treatment and nursing measures of acute skin graft-versus-host disease(aGVHD) following allogeneic HSCT, and put forward relevant management advice, in order to provide reference for clinical nurses in the early period of prevention, early detection and timely intervention on aGVHD. (造血干细胞移植是目前治疗恶性及非恶性难治性血液病的重要方法, 移植物抗宿主病(GVHD)是其主要并发症之一, 严重影响患者预后。本研究通过查阅国内外相关文献, 综述异基因造血干细胞移植皮肤急性移植物抗宿主病(aGVHD)的发病机制、临床分级、预防、治疗和护理措施, 并提出相关管理建议, 以期协助临床医护人员针对皮肤aGVHD的前期预防、早期发现、及时干预提供参考。)

Copyright of Journal of Acupuncture & Tuina Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的：分析针对性护理在面部重度痤疮患者中的应用效果。方法：选取2022年7月-2023年7月本院78例 面部重度痤疮患者开展研究，用随机数字表法平均分为对照组39例，行常规护理，观察组39例，行针对性护理，比较 两组护理效果。结果：观察组的治疗依从性、EF、CF、RF、SF、PF和SSRS评分均明显高于对照组，皮损消失时间、 水肿发生率、SAS、SDS、SPB评分和皮损积分均明显低于对照组（P < 0.05）。结论：给予面部重度痤疮患者针对性 护理能缩短痤疮消失时间，提升生活质量和依从性，具有推广价值。 [ABSTRACT FROM AUTHOR]

Copyright of Detergent & Cosmetics is the property of Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Basal cell carcinoma (BCC) is the most common malignant tumor in dermatology with incidence rising rapidly. Expert consensus on diagnosis and treatment of cutaneous basal cell carcinoma (2021) was published in September 2021 by Skin Tumor Research Center, Chinese Society of Dermatology and Subcommittee on Skin Tumor, China Dermatologist Association. This consensus comprehensively describes the epidemiology, pathogenesis, clinical manifestations, auxiliary examination, pathology, pretreatment assessment, treatment, prognosis, and follow-up education. It offers an important guideline for promoting the standardized diagnosis and treatment of skin BCC in China. In this work, multidisciplinary experts interpreted the main contents of the consensus, including clinicopathological findings, pretreatment assessment, and treatment advance. [ABSTRACT FROM AUTHOR]

This paper introduced the origin of wax therapy technology and its clinical application in cervical spondylosis, osteoarthritis, gynecological diseases, skin diseases, nervous system and digestive system diseases. The clinical application status of wax therapy is summarized as follows, in order to provide more ideas and basis for clinical research. (本文总结了蜡疗技术的起源以及蜡疗技术在颈椎病、骨关节炎、妇科病、皮肤类疾病、神经系统、消化系统疾病中的临床应用, 以期为临床进一步完善蜡疗操作规范提供更多的思路与依据。)

As the first line of defense to protect the human body, skin is susceptible to varying degrees of injury from external forces, heat, disease, and chemical corrosion. Wound dressings constructed based on tissue engineering principles can effectively improve clinical tissue repair, accelerate the wound repair process, relieve patient pain, and reduce scar formation. Silk fibroin, a natural polymer with the advantages of abundant sources, excellent mechanical properties, degradability, and good biocompatibility, is especially favored by researchers in the field of wound repair. In recent years, based on the extensive research on the application of silk fibroin in skin wound repair, the biological properties of silk fibroin for wound repair have been gradually elucidated and become an important reference to guide the design and construction of wound repair materials. Through the design of structure and properties, drug and biological factor loading, and stem cell compounding of silk fibroin dressings, the functions of silk fibroin dressings to prevent wound infection, promote wound healing, and reduce scar regeneration have been realized. Wound healing is a continuous and slow process that is divided into four main stages: hemostasis, inflammation, proliferation and remodeling. Silk fibroin is involved in almost every step of wound healing. Silk fibroin can achieve hemostasis by dissolution or adhesion to the wound and cause a mild inflammatory response during the initial phase of implantation, which facilitates the destruction of pathogens present at the site of injury and accelerates wound repair. Silk fibroin can also promote the expression of fibroblast fibronectin and vascular endothelial growth factor, activate NF-κB signaling pathway, promote vascular neovascularization, develop granulation tissue, and epithelization. With the further development and research on silk fibroin, the functional properties of silk fibroin materials related to wound repair have been gradually elucidated, which has become an important clue for the design and construction of silk fibroin biomaterials and accelerated the application of silk fibroin in wound repair. Silk fibroin has good plasticity and can be processed into films, porous sponges, hydrogels and nanofibers to better meet the needs of various wound repairs. In recent years, silk fibroin dressings have been successfully developed and approved for clinical wound repair in China. In order to accelerate wound healing and enhance the effect of wound repair, important progress has been made in the research of designing and optimizing the structural properties of silk protein materials, or combining them with active factors for wound repair. The lack of bioactivity of pure silk fibroin limits its application in biomedical fields, and better repair results can be achieved through designing and constructing specific structure. For example, the anisotropic structures of scaffolds can improve cell migration efficiency, promote inward tissue growth, epidermal renewal, and faster wound closure. Compounding with natural or synthetic polymers can give silk fibroin materials more potential. Keratin, polyvinyl alcohol, sodium alginate, aloe vera gel, collagen, and hyaluronic acid would all promote wound healing when compounded with silk. Wound healing is a complex and dynamic process involving the participation and regulation of multiple growth factors. The silk fibroin material loaded with growth factors can endow it with more biological activity like promoting cell proliferation and migration, and protein and fiber deposition in the extracellular matrix, thus achieving a better wound repair effect. In addition, silk fibroin is also an excellent drug carrier, and can maintain drug activity and achieve precise drug delivery. The better wound healing can be achieved by loading drugs. For instance, silk fibroin hydrogels loaded with deferoxamine (DFO) can promote cell migration and tissue inward growth, and reduce the cytotoxicity of drugs to accelerate the process of wound healing. To promote better wound healing, silk protein is also usually combined with stem cells to regulate the immune response and cellular microenvironment, so as to promote the expression of wound healing-related genes, and accelerate wound healing and regeneration. With the advancement of science and technology, the development of silk fibroin dressings has become more diversified. The emergence of 3D bioprinting technology, food-breeding method and microneedle dressing technology is bound to bring new insights to the development of new dressings and provide new ideas for the development of functional and active silk protein dressings. With the continuous progress of science and technology, people have deepened their research on the biological properties of silk fibroin, which can promote cell adhesion, re-epithelialization and hemostasis, reduce the immune response, accelerate the process of wound repair, and play an important role in promoting wound healing. As a biomaterial, silk fibroin has been widely researched and applied. Further functionalization of silk fibroin materials through the structural design and performance optimization of silk fibroin itself and the combined use of other bioactive molecules such as growth factors, drugs and stem cells will enhance the efficacy of silk protein dressings and open up more possibilities for their application in skin wound repair, accelerating the research process of silk protein biomaterials in clinical application. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Dalian Ocean University is the property of Journal of Dalian Ocean University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The rotten-skin disease was a common disease in the artificial breeding process of Quasipaa spinosa, which was mainly manifested as the frog epidermal ulceration caused by bacterial infection. The disease was highly infectious and had a high mortality rate, which seriously affected the economic benefits of the breeding of Quasipaa spinosa. Therefore, it was of great significance to analyze the bacterial diversity in the ulcerated skin for the diagnosis and prevention of rotten-skin disease in Quasipaa spinosa. In this study, the skin samples from both healthy and ulcerated parts of Quasipaa spinosa affected with the rotten-skin disease were collected, and the bacterial 16sRNA fragment high-throughput sequencing and bioinformatics analysis were used to explore the bacteria structural characteristics in the ulcerated skin of Quasipaa spinosa affected with rotten-skin disease. The results showed that the abundance and diversity of bacteria in the ulcerated skin were significantly lower than those in the healthy skin. At the phylum taxonomic level, the dominant phylum in the ulcerated skin was Proteobacteria, while the dominant phyla in the healthy skin were Cyanobacteria Chloroplast, Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. At the genus taxonomic level, the dominant genus in the ulcerated skin was Acinetobacterwhile the dominant genera in the healthy skin were Streptophyta and Acinetobacter, Although Acinetobacter was the common genus to both healthy and ulcerated skin, the content of Acinetobacter in the ulcerated skin was significantly higher than that in the healthy skin. The results of this study analyzed the possible pathogenic bacteria in the skin of Quasipaa spinosa affected with the rotten-skin disease, which had important guiding significance for the prevention and treatment of diseases in the breeding process of Quasipaa spinosa. [ABSTRACT FROM AUTHOR]

Copyright of Detergent & Cosmetics is the property of Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

结外 NK/T 细胞淋巴瘤(鼻型)是一种侵袭性结外非霍奇金淋巴瘤, 在亚洲, 拉丁美洲和南美的 发病率高于西方国家, 其中我国占12.0%~17.1%。结外 NK/T 细胞淋巴瘤(鼻型)恶性程度高, 侵袭性强, 进 展快, 病死率高, 患者预后较差。该院收治结外 NK/T 细胞淋巴瘤(鼻型)复发, 累及皮肤合并继发性嗜血细胞 综合征患者1例, 分析诊治过程以探究该病的临床及病理等表现, 旨在减少误, 漏诊, 让患者及时得到救治, 提 高患者的生存率和生活质量。 [ABSTRACT FROM AUTHOR]

细胞外囊泡(extracellular vesicles, EV)作为再生医学和药物递送的亚细胞疗法,近年来广受关注,而血小板作为治 疗性 EV的一个来源,具有独特的优势。血小板来源的细胞外囊泡(platelet extracellular vesicles, P-EV)具有抗凝、抗炎、促进细胞 再生和免疫调节等作用,并具有药物递送载体的潜能,其应用的主要领域包括促进伤口愈合,血管生成,促进神经、肌肉、骨骼的 生成。现对目前P-EV在皮肤再生领域的研究进展作一综述。 [ABSTRACT FROM AUTHOR]

BACKGROUND: It is believed that leptin receptor (LepR) marks the adult bone marrow mesenchymal stem cells in mice and LepR+ cells contribute to the regeneration of fractured bone. However, the residence of LepR+ stromal cells in skin and the function of the population in skin development and repair are poorly understood. OBJECTIVE: To investigate the expression of LepR in mouse skin and the role of LepR+ cells in development and tissue repair. METHODS: The study was approved by the Experimental Animal Ethics and Use Committee of Shanghai Jiao Tong University with the approval No. 1504002. The LepR-Cre; tdTomato mice were generated by using the classic Cre-loxP system for tracing the expression of LepR in mouse skin. Flow cytometry was used to analyze surface markers on Tomato+ cells. A full-thickness skin excision wound model was constructed on the back of LepR-Cre; tdTomato mice to trace the distribution of LepR+ cells in skin wound healing. The LepR-Cre; Tsc1fl/fl mice for specific deletion of Tsc1 in LepR+ cells were constructed to identify the role of Tsc1 in mouse skin development. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

糖尿病足为糖尿病患者严重并发症之一,病情进展后期足部逐渐出现溃疡,由于糖尿病患者本 身血供不良及周围神经病变,伤口愈合非常困难。近年来,有研究将细胞因子用于糖尿病足的治疗中起到显著 疗效,且同时发现联合多种细胞因子可提高疗效。该文就糖尿病足创面皮肤基质相关细胞因子进行了综述,以 推动细胞因子在糖尿病足溃疡治疗方面的应用。 [ABSTRACT FROM AUTHOR]

间充质干细胞是一类来源于人体多种组织的干细胞, 其具有多向分化及分泌相关促生 长因子的潜能。外泌体是间充质干细胞旁分泌的重要活性成分之一。糖尿病患者伤口因局部微环境 改变导致其愈合欠佳。间充质干细胞及外泌体可通过多种途径促进糖尿病伤口愈合。该文就间充质 干细胞及外泌体在促进糖尿病伤口愈合方面的研究进展、间充质干细胞及相关外泌体的应用途径及 局限性进行综述。. [ABSTRACT FROM AUTHOR]

BACKGROUND: Dermal extracellular matrix (d-ECM) has been widely used in clinical wound repair, but whether dermal extracellular matrix hydrogel (d-ECMH) can improve acute skin wound healing is still a lack of relevant research. OBJECTIVE: To explore the healing effect and mechanism of d-ECMH on acute skin wounds in rats. METHODS: Twenty-four SD rats were divided into model group, d-ECM group and d-ECMH group by random number table method, with 8 rats in each group. Firstly, all rats established a full-thickness skin defect model, and then the model group and d-ECMH group were immediately injected with equal volumes of PBS buffer and d-ECMH on the wound respectively after the operation, and the wound surface of the d-ECM group was covered with d-ECM to observe and evaluate wound healing. All rats were sacrificed on day 14, and the skin tissues of the wounds were subjected to hematoxylin-eosin staining, Masson staining, histoimmunochemistry and fluorescence staining. The experiment was approved by Animal Ethics Committee of General Hospital of Western Theater Command. RESULTS AND CONCLUSION: (1) The wound of rats in each group shrank with the prolongation of treatment time. Compared with the model group and the d-ECM group, the d-ECMH group had higher wound healing rate and wound surface skinization rate (P < 0.05), and reduced wound contracture rate (P < 0.05). (2) Hematoxylin-eosin staining showed that compared with the model group and the d-ECM group, the d-ECMH group had better skin wound repair and a higher degree of re-epithelialization of the wound, forming new skin appendages (such as hair follicles), and the matrix of dermis was more uniform. (3) Masson staining results showed that compared with the model and the d-ECM groups, the collagen fibers in the skin wound tissue were significantly increased and thicker in the d-ECMH group. (4) Immunohistochemical staining showed that the number of new capillaries in the skin wound tissue of the d-ECMH group was significantly more than that of the model group and d-ECM group (P < 0.05). The contents of tumor necrosis factor-α and interleukin-6 in wound tissues of the d-ECMH group were significantly lower than those in the model group and d-ECM group (P < 0.05). (5) Immunofluorescence staining showed that the number of M2 macrophages in the new wounds of the d-ECMH group was significantly higher than that of the model group and d-ECM group (P < 0.05), while the number of M1 macrophages was significantly lower than that of the model group and d-ECM group (P < 0.05). (6) The results show that d-ECMH can accelerate skin wound healing, promote wound re-epithelialization, and reduce wound contracture, which may be related to promote capillary angiogenesis and collagen fiber production, reduce inflammation and regulate the polarization of macrophages to M2 in the wound. [ABSTRACT FROM AUTHOR]

BACKGROUND: Tissue-engineered skin has a good application prospect in promoting skin tissue wound repair and scarless healing. OBJECTIVE: To review the current research progress, application and value of tissue-engineered skin in vivo and in vitro. METHODS: A computer was used to search the relevant articles in the CNKI database and PubMed database. The Chinese and English search terms were “mesenchymal stem cells; growth factors; skin; wounds; skin repair; wound healing; regenerative medicine; tissue engineering; scaffolds”. The domestic and foreign related application articles on tissue-engineered skin construction in the treatment of skin tissue injury in recent years were checked, and the research progress of mesenchymal stem cells, growth factors, scaffold materials and dressings for new treatment methods for tissue repair was also summarized. The articles irrelevant to the subject of this article were excluded, and 78 articles were finally included for the result analysis. RESULTS AND CONCLUSION: The construction of tissue-engineered skin involves the selection of seed cells, the application of growth factors, and the combination of multiple elements of scaffold materials. Mesenchymal stem cells are one of the important sources of tissue-engineered skin seed cells. Growth factors optimize the tissue culture environment, and scaffold materials provide appropriate mechanical support. Therefore, how to select appropriate seed cells, growth factors and scaffold materials to construct tissue-engineered skin to treat wounds and speed up repair is the focus and research direction that should be explored in the future. In addition, the development of wound materials is also a new field. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Clinical Healthcare is the property of Chinese Journal of Clinical Healthcare and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: In the skin and hair pigmentation mechanism, diacylglycerol kinase ζ (DGKζ) has not been reported to participate in pigmentation. OBJECTIVE: To investigate the expression and localization of DGKζ and protein kinase C βII (PKCβII) in the mouse back skin with different coat colors and their correlation with the formation of coat color. METHODS: The back skin of 2-month-old mice with different coat colors (white, gray, and black, 6 mice for each color) was taken to detect DGKζ and PKCβII mRNAs by real-time PCR, DGKζ, PKCβII and p-PKCβII proteins by western blot, and DGKζ and p-PKCβII expression by immunohistochemistry. The study protocol was approved by the Animal Ethics Committee of the Animal Experimental Center of Shanxi Medical University. RESULTS AND CONCLUSION: DGKζ and PKCβII mRNAs were both expressed in the back skin of mice with three kinds of coat colors, with its relative expression amount being: gray group > white group; black group > white group (P < 0.05). Both DGKζ and PKCβII proteins were expressed in the back skin of mice with different hair coat colors. The ratio of p-PKCβII to PKCβII showed that the change of PKCβII activity had the same tendency with DGKζ expression: gray group > white group; black group > white group (P < 0.05). Immunohistochemistry staining showed that DGKζ and p-PKCβII were positively expressed in the outer root sheath of hair follicle, hair matrix cells and melanocytes in the hair matrix in the back skin of gray and black mice, while were negative in the hair follicles of white mice. According to the expression and localization of DGKζ, PKCβII in white, gray and black mouse back skin, we can speculate that DGKζ and PKCβII may participate in the formation of the coat color. [ABSTRACT FROM AUTHOR]

The present paper aimed to study the structure and function of ASIP gene and its coding protein in Youzhoudark goat ( YZD goat) skin tissues. [ Method] YZD goat ASIP gene was cloned by 5' and 3 'RACE from skin tissues and analyzed by the bioinformatics. [ Result] A 787 bp sequence ( Pl) was identified as YZD goat ASIP gene including 209 bp 5' UTR, 402 bp CDS, and 209 bp 3' UTR. It encoded 133 amino acids and was predicted to be an unstable hydrophilic protein, and a signal peptide of about 22 amino acids. In addition, a missense substitution ( c. 676A > G) has been found in YZD goat ASIP gene, leading to the convert from the alanine to valine ( p. Ala96V al), located in the Agouti super family conserved domain. Meanwhile, the mis sense substitution of amino acids also caused the difference of spatial conformation of YZD goat ASIP. [ Conclusion ] The sequence characteristics and protein structure of YZD goat ASIP gene were analyzed and it could be concluded that the missense substitution of ASIP gene coding region in YZD that goat may have an effect on skin pigmentation. [ABSTRACT FROM AUTHOR]

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Interferon is a kind of cellular regulatory substance similar to polypeptide hormone, which has a wide range of biological activities, such as antivirus, anti-proliferation, antitumor and immunomodulatory functions. OBJECTIVE: To review the effect of interferon α-2b to inhibit scar formation in multiple organs, so as to provide a theoretical basis for clinical treatment of scar and fibrosis diseases. METHODS: A computer-based online search of WanFang, VIP, CNKI, PubMed, Web of Science and Elsevier Science Direct databases was performed to retrieve papers published during 1950-2020 with the search terms of “IFNα-2b, interferon α-2b, fibroblasts, scar, hypertrophic scar, keloid” in Chinese and English, respectively. A total of 42 papers were included for the final analysis. RESULTS AND CONCLUSION: Interferon α-2b can inhibit the occurrence and development of scar, reduce the production of extracellular matrix collagen, inhibit the proliferation of fibroblasts, and act on cytokines and gene targets related to fibrosis. Findings from this study provide a theoretical basis for the clinical application of interferon α-2b in the treatment of scar formation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Vitrification-based cryopreservation is a promising cryopreservation method, which can change the state of the biological materials by using high concentration vitrification reagent to realize active preservation. OBJECTIVE: To review the biological principle of vitrification-based cryopreservation, the classification of cryopreservation reagents, as well as the cryopreservation of ovary skin, cornea and other medical tissue specimens. METHODS: PubMed and WanFang databases were searched for relevant articles published from January 1994 through October 2019. Search terms were “tissue; vitrification; cryopreservation” in English and Chinese, respectively. According to the inclusion and exclusion criteria, 45 articles were finally included in result analysis. RESULTS AND CONCLUSION: The process of vitrification-based cryopreservation can avoid ice crystallization by which the cells are damaged, and effectively preserve the cell’s biological activity and basic functions. Vitrified cryopreservation reagents can be divided into permeable and non-permeable reagents. Their operation is simple and efficient. The only disadvantage is that the high concentration of cryopreservation reagents can cause some toxic injuries to the cells. To reduce the risk of overall tissue damage, a variety of low-toxic cryopreservation reagents can be mixed and used. At present, vitrification-based cryopreservation technology has been successfully applied in a variety of cells. However, the technical problems in the cryopreservation of tissues have not been solved completely. [ABSTRACT FROM AUTHOR]

Vulvar Paget disease is a rare malignant tumor of the skin that usually occurs in postmenopausal women. It causes symptoms such as itching, local pain or burning sensation of the vulva. It usually presents as erythema or eczema-like changes of the skin of the vulva. The diagnosis is confirmed by pathological biopsy. Vulvar Paget disease can be classified into primary and secondary vulvar Paget disease. Immunohistochemical stains can be used to make a relatively clear differential diagnosis of vulvar Paget disease. Due to its non-specific clinical manifestations and atypical pathological features, the rate of misdiagnosis and delayed diagnosis is high. Surgical treatment is the first choice for the treatment of vulva Paget disease. However, the formulation of surgical scope is a difficulty of current treatment. If the surgical scope is too large, it may cause serious tissue damage and dysfunction. Topical imiquimod cream and photodynamic therapy have provided new ideas for the conservative treatment in recent years. Regardless of surgical treatment or conservative treatment, the recurrence rate of vulvar Paget disease is high. Its prognosis is closely related to pathological type, lymph node metastasis and infiltration depth. It is necessary to conduct long-term follow up for patients with the disease. This article will review the progress in diagnosis and treatment of vulva Paget disease. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To study the effects of different treatment methods of platelet-rich plasma (PRP) on skin wound healing in mice. Methods A total of 27 C57BL/6J male mice were used in this study, 12 of them were used to prepare PRP, and the other 15 mice were randomly divided into control group, PRP injection group and PRP gel group. There were 5 mice in each group. Models of skin injury were established in all three groups of mice. For the PRP injection group, the skin wound was equally divided into 6 points and subcutaneously injected PRP with 0.1 mL at each point. The control group was injected the same amount of saline with the same method. For the PRP gel group, PRP was activated to prepare gel to cover the skin wounds of mice. All three groups were given medicine only once on the day after surgery. The wound areas were photoed and calculated on the 0, 3, 7, 11, and 14 day respectively. On the 14th day, mice of the three groups were sacrificed and the skin tissues around the wound were collected. The collagen expression was evaluated by immunofluorescence staining. The mRNA expressions of collagen type Ⅰ alpha 1 chain (COL1A1), collagen type Ⅲ alpha 1 chain (COL3A1), transforming growth factor-beta 1 (TGF-β1), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) in the wound site were detected by qRT-PCR. Results Compared with the control group and the PRP injection group, the skin wounds healed significantly and the wound area decreased in the PRP gel group at different time points after surgery (P＜0.05). At 11 and 14 days after surgery, the wound area decreased in the PRP injection group compared with that of the control group (P＜0.05). At 14 days after operation, collagen expressions were significantly increased in the PRP gel group compared with those of control group and the PRP injection group, and mRNA levels of VEGF, COL1A1, COL3A1, TGF-β1 and PDGF increased (P＜0.05). Conclusion The preparation of gel from the PRP activation can effectively repair the skin defects of mice. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)