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轻型卒中的定义及诊断标准尚不统一，其是否都需要溶栓尚存在争议。轻型卒中在我国卒中患者中的比例有逐年增加的趋势，虽然有基于氯吡格雷用于急性非致残性脑血管病事件高危人群（clopidogrel in high-risk patients with acute non-disabling cerebrovascular events，CHANCE）系列研究抗血小板治疗的循证医学证据，但仍有30%的患者预后不良，由此带来的疾病负担仍然很重。当前指南对于发病4.5 h内的轻型致残性卒中推荐静脉溶栓治疗，而对于轻型非致残性卒中意见不一。近年的研究显示，对于轻型卒中合并大血管闭塞患者，早期包括静脉溶栓在内的积极再灌注治疗可能会给患者带来功能获益。本文对轻型卒中溶栓相关研究进行综述，期待未来有更多的设计严谨的大型试验来评估溶栓治疗在轻型卒中患者中的有效性和安全性。 Abstract: The definition and diagnostic criteria of minor stroke are not unified until now, and it is still controversial whether all patients with acute minor stroke need intravenous thrombolysis. The proportion of minor stroke patients in China has an increasing trend year by year. Although the CHANCE (clopidogrel in high-risk patients with acute non-disabling cerebrovascular events) trial and other studies have showed that antiplatelet therapy is effective for minor stroke, there are still approximately 30% of patients with poor functional outcomes and the burden of disease is very heavy. The current guidelines recommended intravenous alteplase administration within 4.5 hours for patients with disabling minor stroke but not for nondisabling minor stroke. Recent studies showed that reperfusion therapy including intravenous thrombolysis might be beneficial for patients with minor stroke and large vessel occlusion. This article reviewed the advances in thrombolytic therapy for minor stroke, to provide reference for clinical treatment and relevant research in minor stroke.

目的 对比远端桡动脉入路（distal transradial artery access，dTRA）与常规桡动脉入路（transradial approach，TRA）行DSA的可行性和安全性，优化神经介入治疗的穿刺入路策略。 方法 前瞻性连续纳入2022年7月—2023年1月北京市大兴区人民医院神经内科拟行DSA的患者，按计算机产生随机数字的方法，将患者分为dTRA组和TRA组。将按照预定入路成功完成穿刺的患者纳入分析，收集两组患者的人口学信息、既往病史等基线资料，比较两组患者穿刺血管内径、穿刺时间、穿刺次数等手术过程指标，以及手臂疼痛、麻木、肿胀、桡动脉闭塞、动静脉瘘等术后并发症情况。利用多因素logistic回归模型分析影响dTRA首次穿刺成功率的影响因素。 结果 本研究共纳入320例拟行DSA的患者，其中306例患者纳入分析，dTRA组和TRA组各153例患者。两组患者的基线资料差异无统计学意义。dTRA组的穿刺血管内径较TRA组更小[（1.97±0.42）mm vs.（2.23±0.44）mm，P＜0.001]。两组患者首次穿刺成功率差异无统计学意义，但与TRA组相比，dTRA组的穿刺时间更长（P=0.008）、穿刺次数更多（P=0.022）、总手术时间更长（P=0.003）、射线剂量更大（P=0.027）。与TRA组相比，dTRA组术后手臂疼痛评分更低（P＜0.001），麻木、肿胀发生率更低（P＜0.001），桡动脉闭塞发生率更低（P=0.002）；TRA组患者术后均未发生动静脉瘘，dTRA组有2例患者发生动静脉瘘，差异无统计学意义。多因素logistic回归模型分析显示，远端桡动脉内径（OR 3.860，95%CI 1.364～10.924，P=0.011）及心房颤动病史（OR 0.121，95%CI 0.018～0.831，P=0.032）是影响dTRA首次穿刺成功率的危险因素。 结论 与TRA相比，dTRA可降低患者桡动脉闭塞、麻木、肿胀以及疼痛等并发症的发生率，提高患者舒适度。 Abstract: Objective To compare the feasibility and safety of DSA between distal transradial artery access (dTRA) and transradial approach (TRA), and to optimize the puncture approach strategy for neurointerventional therapy. Methods Patients who planned to undergo DSA in the Department of Neurology, Daxing District People’s Hospital from July 2022 to January 2023 were continuously included. According to the method of generating random numbers by computer, the patients were randomly divided into the dTRA group and the TRA group. Patients who completed puncture according to the predetermined approach were included in the analysis. Demographic information, past medical history, and other baseline data of the two groups were collected. The internal diameter of puncture vessels, puncture time, puncture times, and other surgical process indexes, as well as postoperative complications such as arm pain, numbness, swelling, radial artery occlusion, and arteriovenous fistula, were compared between the two groups. A multivariate logistic regression model was used to analyze the influencing factors on the success rate of dTRA first puncture. Results In this study, 320 patients who intend to undergo DSA were included, of which 306 patients were included in the data analysis, with 153 patients in the dTRA group and 153 patients in the TRA group. There was no statistical difference in baseline data between the two groups. The internal diameter of puncture vessels in the dTRA group was smaller than that in the TRA group [(1.97±0.42) mm vs. (2.23±0.44) mm, P

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Copyright of Journal of Petrochemical Universities / Shiyou Huagong Gaodeng Xuexiao Xuebao is the property of Journal Editorial Department Of Liaoning Shihua University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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The objective of this research is to explore the effects of the G0S2 gene on the cell proliferation of ovarian granulosa cells, the secretion of estrogen（E2）and progesterone（P4）, as well as the expressions of steroid secretion-related genes and apoptosis genes. Fresh ovaries of small-tailed sheep were collected,and the follicular fluid gathered from small and medium-sized follicles by the suction method was centrifuged to separate the granulosa cells into experimental and control groups. The G0S2 gene in the experimental group was knocked out by CRISPR-Cas9 gene editing technology and the obtained recombinant expression vector PX458-sgRNA-G0S2 was transfected to the granulosa cells. The PX458 plasmid in the control group was transfected to the granulosa cells. The viability and apoptosis of the granulosa cells were detected, as well as the E2 and P4 concentrations and associated gene expression. The results showed that the relative abundance and the protein level of G0S2 mRNA in the experimental group were lower（P<0.01）than those in the control group, reduced by 66% and 70% respectively, proving that the G0S2 was successfully knocked out. The proliferation activity of the granulosa cells in the experimental group was higher（P<0.05） than the control group, and the apoptosis rate reduced（P<0.01）by 56%. Compared with the control group, the E2 concentration was higher （P<0.05）and the P4 concentration was lower（P<0.05）in the experimental group. The expressions of genes StAR, CYP11 and 3β-HSD in the experimental group was lower（P<0.01）than the control group, and the expressions of CYP19 was significantly higher（P<0.01）than the control group. Compared with the control group, the expression of Caspase3 and Bax in the experimental group of granulosa cells was significantly downregulated（P<0.01）, and the expressions of Bcl-2 was significantly upregulated（P<0.01）. Our results demonstrate that knockout of the G0S2 gene can promote the proliferation of ovarian granulosa cells, inhibit their apoptosis, and affect the secretion of E2 and P4 by downregulating the expression of genes StAR, CYP11, 3β-HSD and upregulating CYP19 in sheep. [ABSTRACT FROM AUTHOR]

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To investigate the effects of different biochar applications on soil enzyme activities in Eucalyptus plantations in North Guangxi, and provide theoretical references for the rational application of biochar to Eucalyptus plantations. Based on field experiments, biochar was prepared anaerobically at 500 ℃ from branches of Eucalyptus plantation harvesting residues, and with six treatment groups, such as 0 (CK), 0.5% (T1), 1% (T2), 2% (T3), 4% (T4), and 6% (T5), to analyze the changes in soil enzyme activities under different application rates after one year importation. The results were as follows: (1) The soil enzyme content decreased gradually as the soil layer deepened. (2) In the same soil layer, the contents of urease, catalase, β-glucosidase and dehydrogenase increased with the increase of biochar application, and the highest contents were found at 6% of biochar application. (3) With the increase of biochar application, the contents of acid phosphatase, sucrase, leucine aminopeptidase and cellobioglucosidase showed a trend of first increasing and then decreasing. Acid phosphatase and leucine aminopeptidase had the highest contents at 2% of biochar application. Sucrase and cellobioglucosidase had the highest contents at 4% of biochar application. In general, the biochar application increased the soil enzyme activities of Eucalyptus plantations. The results of this study can provide a scientific basis for the application of forestry waste biochar in Eucalyptus plantations. [ABSTRACT FROM AUTHOR]

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Copyright of Feed Research is the property of Editorial Board of Feed Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Reference crop evapotranspiration (ET0) is one of the most important hydroclimatic variables for the hydrologic and crop models, as well as the actual evapotranspiration in the irrigation schedule. The ET0 prediction a few months in advance can be beneficial to the decision-making on the long-term planning in the water management and irrigation communities. In this research, the monthly predictions of ET0 were realized over the Huaihe River basin using the model data of Beijing Climate Center second-Generation Climate Prediction System (BCC_CPSv2), and the surface meteorological observations at 172 stations during 1991 to 2020. The bilinear interpolation and quantile mapping were also selected to downscale and then correct the mean air temperature, net radiation, relative humidity, and wind speed of BCC_CPSv2. The obtained four variables were used to calculate the ET0 predictions using the Penman-Monteith equation. The correlation coefficient (r), Mean Bias Error (MBE), Root Mean Square Error (RMSE), and Mean Absolute Percentage Error (MAPE) were employed to evaluate the ET0 prediction performances of the model, and the four climatic variables before and after correction. Results showed that the mean air temperature, net radiation, and relative humidity from the model were significantly smaller than those from the observations before correction in each month with the RMSE of 1.84℃, 1.70 MJ/(m2 ·d), and 15.79%, respectively. The wind speed was also smaller during March to June, but larger in other months, with the RMSE of 1.39 m/s. Errors in the climatic variables led to the lower ET0 during February to June, and the larger ET0 in January, as well as in July to December, compared with the calculations. The RMSE of ET0 before correction was ranged from 0.11 to 1.70 mm/d for each month, among which was the highest in summer. The larger RMSE exceeding 0.50 mm/d occurred in eastern coastal areas in January and October, while the southwestern mountainous areas presented the higher RMSE exceeding 0.70 mm/d in April and July. The model skills on predicting climatic variables and ET0 were improved effectively by the quantile mapping. The RMSE of air temperature, net radiation, relative humidity, and wind speed decreased to 1.32℃, 0.74 MJ/(m2 ·d), 7.38%, and 0.53 m/s, respectively. Especially, the MAPE of wind speed and net radiation was decreased by more than 19%. The RMSE of ET0 after correction was ranged between 0.05 and 1.22 mm/d for each month, indicating the decrease in 80% of the months. The performances of the model were improved significantly for the eastern coastal areas in January, and the southwest mountainous areas in April, July and October, where the RMSE decreased by more than 0.30 mm/d. Before and after the correction, the net radiation and relative humidity were the primary factors for the ET0 prediction errors in summer half year and winter half year, respectively, due to the larger errors of the model and the higher sensitivity of ET0 to them. A better performance was achieved in the monthly ET0 prediction after the model correction with the error about 10.7%. The finding can provide a strong reference for the water resources management, irrigation schedule planning, and agricultural water demand decision-making. [ABSTRACT FROM AUTHOR]

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In order to analyze the change regulation of regional drought and flood, based on the standard precipitation evapotranspiration index (SPEI) that can effectively evaluate the drought and flood degree quantitatively, the frequency of drought and flood was studied by using in-formation diffusion technology which was good at small sample data analysis, and the chaotic time series prediction model based on information diffusion adding-weight one-rank local-region method was proposed to analyze the trend of drought and flood time series. Taking Xi' an as a typical region and September as the research period, the method suggested in this paper was used for analysis, according to the temperature and precipitation data from 1951 to 2015. The results show that a) the probability of moderate drought or above is about 19.5%, while the probability of moderate wet or above is about 17.5%. b) During 1951-1962, the trend is drought. During 1963-1986, there is a trend of wet. From 1987 to 2002, there is a trend of drought. After 2003, the trend of wet appears again. c) The maximum Lyapunov exponent of drought and flood series is 0.149 2, which shows that the time series of drought and wet have chaotic characteristics. The new chaotic prediction model is used to predict the trend of drought and flood from 1985 to 2002, which is in good agreement with the actual situation and verifies the effectiveness of the method. [ABSTRACT FROM AUTHOR]

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Objective: To explore the method, experience and effect of psychotherapy for patient with synthet ic cannabis addiction. Methods: Based on early rehabilitation skills training, relapse prevention and family therapy in Matrix outpatient intensive intervention, 16 sessions were conducted in 8 weeks to the adolescent patient The urine drug test result, self-reporting and family observation was used to evaluate abstinence duration before and after treat ment. And Cogstate test battery was used to evaluate the change of patient's cognitive function before and after treatment. Results: The patient maintained in abstinence for 8 months after treatment. The accuracy of Cogstate tasks, including verbal learning and memory, spatial problem-solving and error monitoring, working memory and spatial working memory improved, and the number of error decreased after treatment. Conclusion: The Matrix out patient intensive model may contribute to the recovery of patient with synthetic cannabis addiction, which could pro mote cognitive function recovery and maintain abstinence. [ABSTRACT FROM AUTHOR]

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To study the characteristics of soil microbial activity of different forest age of Eucalyptus, using the space-lime intergenerational method, we studied the variations characteristics of soil microbes and enzyme activities from four soil depths (0- 10, 10-20, 20-30 and 30-40 cm) of different forest ages of Eucalyptus plantations (1, 2, 3, 4, 5 a) at the hilly areas in North Cuangxi. The results were as follows: (1) In the soil layers, soil microbial and enzyme activities had obvious vertical distribution characteristics. Both microbial contents and enzyme activities decreased with The deepening of the soil layer, with significant differences among the soil layers. (2) The order of bacteria and actinomycetes contents were autumn > summer > spring > winter, the variation tendency of fungi was spring > summer > autumn > winter, and the enzyme activity changed with season differently, which was higher in summer and autumn, and lower in spring and winter. (3) The soil bacteria, fungi, urease and catalase in the Eucalyptus plantations showed a trend of decreasing first and then increasing with the increase of forest ages, and actinomycetes decreased, increased and then decreased. The activities of invertase and acid phosphatase all increased with the increase of forest ages. (4) There was the significant correlation between the soil microbes and the soil enzymes, indicating that the interaction between soil microbes and soil enzymes had a dose relationship, both of which affected the quality of the soil. The seasonal changes of soil microbes and enzyme activities were different in soil layers, which was the lowest in the winter and was mainly related to temperature, water condition and nutrient return of litter and so on. The interactions between different seasons, soil layers and forest ages had significant effects on soil microbes and enzyme activities . [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)